Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the influence of oxygen on the reduction on nitrate by adapted cells of pseudomonas denitrificans. | 1957 | 13413731 | |
| the influence of oxygen availability on the degree of nitrate reduction by pseudomonas denitrificans. | 1957 | 13437212 | |
| production and utilization of nitrous oxide by pseudomonas denitrificans. | 1959 | 13620649 | |
| taxonomic relationships among the pseudomonads. | colwell, r. r. (university of washington, seattle), and j. liston. taxonomic relationships among the pseudomonads. j. bacteriol. 82:1-14. 1961.-an electronic computer technique, utilizing the adansonian principle that every feature should have equal weight, was applied in an effort to derive a taxonomy of the pseudomonas-achromobacter group of gram-negative, asporogenous, rodlike bacteria. the validity of the general method was tested by an analysis of 40 well defined strains, principally derive ... | 1961 | 13694873 |
| oxidative phosphorylation coupled with nitrate respiration with cell free extract of pseudomonas denitrificans. | 1963 | 13939676 | |
| effect of hemin and oxygen tension on growth and nitrate reduction by bacteria. | jacobs, n. j. (american meat institute foundation, chicago, ill.), r. e. heady, j. m. jacobs, k. chan, and r. h. deibel. effect of hemin and oxygen tension on growth and nitrate reduction by bacteria. j. bacteriol. 87:1406-1411. 1964.-the effect of hemin supplementation of growth media on the ability of several bacteria to reduce nitrate was studied. added hemin had no detectable effect on the ability of these organisms to reduce nitrate when grown in stationary cultures exposed to air. however, ... | 1964 | 14188721 |
| [studies on the carbon metabolism of pseudomonas fluorescens and pseudomonas denitrificans]. | 1962 | 14459123 | |
| structure/function studies on a s-adenosyl-l-methionine-dependent uroporphyrinogen iii c methyltransferase (sumt), a key regulatory enzyme of tetrapyrrole biosynthesis. | the crystallographic structure of the pseudomonas denitrificans s-adenosyl-l-methionine-dependent uroporphyrinogen iii methyltransferase (sumt), which is encoded by the coba gene, has been solved by molecular replacement to 2.7a resolution. sumt is a branchpoint enzyme that plays a key role in the biosynthesis of modified tetrapyrroles by controlling flux to compounds such as vitamin b(12) and sirohaem, and catalysing the transformation of uroporphyrinogen iii into precorrin-2. the overall topol ... | 2004 | 15522295 |
| structured model for denitrifier diauxic growth. | we present a model for diauxic growth of denitrifying bacteria in which nitrate reductase synthesis kinetics dominate the overall growth kinetics. the model is based on the assumption of the existence of a nitrate respiration operon, thereby linking the rate of nitrate uptake to the activity of nitrate reductase. we show that this approach can model diauxic growth of pseudomonas denitrificans by conducting experiments in which nitrate reductase activity was measured during both lag and ensuing e ... | 2005 | 15803468 |
| a simple model for diauxic growth of denitrifying bacteria. | a simple model has been formulated to simulate diauxic growth of denitrifying bacteria. it is capable of fitting the experimental results of batch growth experiments with pseudomonas denitrificans under various conditions. it successfully predicts the observed lags when a pure culture of this bacterium switches from oxygen to nitrate as terminal electron acceptor. the model includes the effect of carbon substrate limitation and length of aerobic phase and does not run into problems when switchin ... | 2005 | 15899290 |
| fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter. | spectrophotometric determination of the hydrolysis of fluorescein diacetate (fda) was shown to be a simple, sensitive, and rapid method for determining microbial activity in soil and litter. fda hydrolysis was studied in soil and straw incubated for up to 3 h. hydrolysis was found to increase linearly with soil addition. fda hydrolysis by pure cultures of fusarium culmorum increased linearly with mycelium addition both in shake cultures and after inoculation into sterile soil. fda hydrolysis by ... | 1982 | 16346026 |
| dependence of betaine stimulation of vitamin b(12) overproduction on protein synthesis. | the betaine-stimulated differential synthesis of vitamin b(12), i.e., the increase in b(12) per increase in dry cell weight, by pseudomonas denitrificans was inhibited by rifampin and chloramphenicol but not by benzylpenicillin and carbenicillin at concentrations of antibiotic that inhibit growth. the level of the first enzyme of corrin (and porphyrin) biosynthesis, delta-aminolevulinic acid synthetase, was decreased to a much greater degree by rifampin and chloramphenicol than by the penicillin ... | 1984 | 16346536 |
| effect of chloramphenicol on denitrification in flexibacter canadensis and "pseudomonas denitrificans". | it was recently reported that chloramphenicol inhibits existing denitrification enzyme activity in sediments and carbon-starved cultures of "pseudomonas denitrificans." therefore, we studied the effect of chloramphenicol on denitrification by flexibacter canadensis and "p. denitrificans." production of n(inf2)o from nitrate by f. canadensis cells decreased as the concentration of chloramphenicol was increased, and 10.0 mm chloramphenicol completely inhibited n(inf2)o production. "p. denitrifican ... | 1995 | 16534926 |
| real-time quantitative lamp (loop-mediated isothermal amplification of dna) as a simple method for monitoring ammonia-oxidizing bacteria. | loop-mediated isothermal amplification (lamp) of dna is a novel technique for the amplification of dna under isothermal conditions. for the first time, we applied this method to develop a simple and quantitative monitoring method for environmental microorganisms targeting amoa gene in ammonia-oxidizing bacteria. quantitative analysis was performed first by measuring fluorescence derived from an intercalation dye using a real-time thermal cycler, and then by measuring the turbidity of the reactio ... | 2006 | 16790287 |
| effect of carbon substrate on electron acceptor diauxic lag and anoxic maximum specific growth rate in species with and without periplasmic enzyme. | the effect of oxidation state of carbon substrate on the diauxic lag of facultative anaerobic denitrifying bacteria growing aerobically upon switching to anoxic growth was studied. also studied was the effect on the anoxic maximum specific growth rate. two pure bacteria cultures were used, paracoccus pantotrophus, denitrifying bacteria containing a periplasmic nitrate reductase (nap), and pseudomonas denitrificans, denitrifying bacteria lacking the periplasmic nitrate reductase. the anoxic maxim ... | 2007 | 17129955 |
| mechanism of the ring contraction process in vitamin b12 biosynthesis by the anaerobe propionibacterium shermanii under aerobic conditions. | the mechanism of the ring contraction process during vitamin b(12) biosynthesis by the anaerobe propionibacterium shermanii was investigated under both aerobic and anaerobic conditions by means of feeding experiments with delta-amino[1-(13)c]levulinic acid (a biosynthetic intermediate of tetrapyrrole) and delta-amino[1-(13)c,1,1,4-(18)o(3)]levulinic acid in combination with (13)c-nmr spectroscopy. we showed that the characteristic mechanism of the ring contraction process (the generation of prec ... | 2007 | 17561959 |
| azurin: x-ray data for crystals from pseudomonas denitrificans. | azurin, a blue-colored copper protein, from a soil bacteria, pseudomonas denitrificans, has been crystallized and its molecular weight of 16,000 was confirmed by means of x-ray difraction. | 1969 | 17840685 |
| an effective and simplified ph-stat control strategy for the industrial fermentation of vitamin b(12) by pseudomonas denitrificans. | in order to improve the productivity of vitamin b(12) by pseudomonas denitrificans carried out in a 120-m(3) fermenter, the effect of ph on vitamin b(12) biosynthesis was investigated. results obtained from shake flask experiments showed that the feeding of carbon source (beet molasses or glucose) and methyl-group donor (betaine or choline chloride) significantly influenced the ph and the biosynthesis of vitamin b(12). in contrast to beet molasses or choline chloride, using glucose as a feed med ... | 2008 | 18320234 |
| improved large-scale production of vitamin b12 by pseudomonas denitrificans with betaine feeding. | the strategy of betaine control for vitamin b12 large-scale fermentation by pseudomonas denitrificans was investigated in this paper. the results obtained in shake-flask experiments demonstrated that betaine could greatly stimulate vitamin b12 biosynthesis but had an inhibition to cell growth. based on the influence of betaine on the fermentation of p. denitrificans, betaine feeding was a beneficial strategy to solve the inconsistency between cell growth and vitamin b12 production. as a result, ... | 2008 | 18440227 |
| reduction of nitrate and nitrite in a cyclically operated continuous biological reactor. | biological reduction of nitrate and nitrite was studied with a continuously operated cyclic reactor. the medium was fed to the reactor during the first phase of the cycle, and the effluent was drawn from the reactor during the third phase of the cycle; reaction occurred throughout the cycle. the process was described mathematically based on kinetic expressions revealed in an independent study. the model equations were subjected to detailed analysis with numerical codes based on the bifurcation t ... | 1995 | 18623275 |
| fundamental denitrification kinetic studies with pseudomonas denitrificans. | fundamental kinetic studies on the reduction of nitrate, nitrite, and their mixtures were performed with a strain of pseudomonas denitrificans (atcc 13867). methanol served as the carbon source and was supplied in excess (2:1 mole ratio relative to nitrate and/or nitrite). nitrate and nitrite served as terminal electron acceptors as well as sources of nitrogen for biomass synthesis. the results were explained under the assumption that respiration is a growth-associated process. it was found that ... | 1995 | 18623364 |
| [biosynthesis, fermentation and application of vitamin b12--a review]. | vitamin b12 is an important nutrient widely used in feed, food and medicine field. china is the primary producing area and the vb12 production is 27 t in 2007, 77% of total production in the world. vb12 is the most complex small molecule difficult to chemosynthesize. it ismanufactured by bacteria and archaea via two alternative routes, aerobic or anaerobic pathway. the main strains used in industry fermentation are propionibacterium freudenrechii and pseudomonas denitrificans. the basic characte ... | 2008 | 18807971 |
| demonstration that cobg, the monooxygenase associated with the ring contraction process of the aerobic cobalamin (vitamin b12) biosynthetic pathway, contains an fe-s center and a mononuclear non-heme iron center. | the ring contraction process that occurs during cobalamin (vitamin b(12)) biosynthesis is mediated via the action of two enzymes, cobg and cobj. the first of these generates a tertiary alcohol at the c-20 position of precorrin-3a by functioning as a monooxygenase, a reaction that also forms a gamma lactone with the acetic acid side chain on ring a. the product, precorrin-3b, is then acted upon by cobj, which methylates at the c-17 position and promotes ring contraction of the macrocycle by catal ... | 2009 | 19068481 |
| biocontrol: bacillus penetrans and related parasites of nematodes. | bacillus penetrans mankau, 1975, previously described as duboscqia penetrans thorne 1940, is a candidate agent for biocontrol of nematodes. this review considers the life stages of this bacterium: vegetative growth phase, colony fragmentation, sporogenesis, soil phase, spore attachment, and penetration into larvae of root-knot nematodes. the morphology of the microthallus colonies and the unusual external features of the spore are discussed. taxonomic affinities with the actinomycetes, particula ... | 1980 | 19300701 |
| effect of periplasmic nitrate reductase on diauxic lag of paracoccus pantotrophus. | paracoccus pantotrophus expresses two nitrate reductases-membrane bound nitrate reductase (nar) and periplasmic nitrate reductase (nap). in growth experiments with two denitrifying species (paracoccus pantotrophus and alcaligenes eutrophus) that have both nap and nar and two species (pseudomonas denitrificans and pseudomonas fluorescens) with nar only, it was found that diauxic lag is shorter for bacteria that express nap. in p. pantotrophus, napedabc encodes the periplasmic nitrate reductase. t ... | 2009 | 19399903 |
| improved vitamin b(12) production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process. | effects of different oxygen transfer rates (otr) on the cell growth and vitamin b(12) biosynthesis of pseudomonas denitrificans were first investigated under dissolved oxygen limiting conditions. the results demonstrated that high otr accelerated cell growth and initial vitamin b(12) biosynthesis rate, while lower otr was critical for higher productivity in the late fermentation process. the oxygen uptake rates (our) corresponded well with otr. based on the metabolic intermediate analysis, a ste ... | 2010 | 20022743 |
| influence of alternating oxic/anoxic conditions on growth of denitrifying bacteria. | denitrifying bacteria that are switched from oxic to anoxic conditions can experience diauxic lag, which is the time required for re-synthesis of nitrate reductase and other denitrifying enzymes. pseudomonas denitrificans were exposed to alternating oxic/anoxic phases in a continuous flow reactor with either 4-h or 8-h anoxic phase lengths, in comparison to a measured diauxic lag of 9.5h following steady-state oxic conditions. the p. denitrificans were unable to sustain anoxic growth at either o ... | 2010 | 20045547 |
| development of amplified fragment length polymorphism-derived functional strain-specific markers to assess the persistence of 10 bacterial strains in soil microcosms. | to augment the information on commercial microbial products, we investigated the persistence patterns of high-priority bacterial strains from the canadian domestic substance list (dsl). specific dna markers for each of the 10 dsl bacterial strains were developed using the amplified fragment length polymorphism (aflp) technique, and the fates of dsl strains introduced in soil were assessed by real-time quantitative pcr (qpcr). the results indicated that all dna markers had high specificity at the ... | 2010 | 20817796 |
| identification and phylogenetic characterization of cobalamin biosynthetic genes of ensifer adhaerens. | ensifer adhaerens csba was screened as a cobalamin producer. the draft genome sequence revealed that the strain possesses 22 cobalamin biosynthetic genes (cob genes). the cob gene arrangement on the genome of e. adhaerens csba was similar to that of other ensifer species, and most similar to that of pseudomonas denitrificans sc510. the cobn sequence phylogeny was generally congruent with that of the 16s rrna gene, and it is suggeted that e. adhaerens csba might have inherited the cob genes from ... | 2012 | 23257908 |
| cloning, expression and characterization of 3-hydroxyisobutyrate dehydrogenase from pseudomonas denitrificans atcc 13867. | the gene encoding an nad(+)-dependent, 3-hydroxyisobutyrate dehydrogenase (3hibdh-iv) from pseudomonas denitrificans atcc 13867 was cloned and expressed in escherichia coli bl 21 (de3) and characterized to understand its physiological relevance in the degradation of 3-hydroxypropionic acid (3-hp). the deduced amino acid sequence showed high similarity to other 3-hydroxyisobutyrate dehydrogenase isozymes (3hibdhs) of p. denitrificans atcc 13867. a comparison of 3hibdh-iv with its relevant enzymes ... | 2013 | 23658760 |
| complete genome sequence of pseudomonas denitrificans atcc 13867. | pseudomonas denitrificans atcc 13867, a gram-negative facultative anaerobic bacterium, is known to produce vitamin b12 under aerobic conditions. this paper reports the annotated whole-genome sequence of the circular chromosome of this organism. | 2013 | 23723394 |
| production of 3-hydroxypropionic acid from glycerol by recombinant pseudomonas denitrificans. | 3-hydroxypropionic acid (3-hp) can be produced from glycerol through two sequential enzymatic reactions that are catalyzed by a coenzyme b12 -dependent glycerol dehydratase and an nad(p)(+) -dependent aldehyde dehydrogenase (aldh), respectively. pseudomonas denitrificans synthesizes coenzyme b12 under aerobic conditions, where nad(p)(+) is regenerated efficiently. hence, it is considered an ideal host for the production of 3-hp from glycerol under aerobic conditions. in this study, recombinant s ... | 2013 | 23775313 |
| structure of nitrogen-converting communities induced by hydraulic retention time and cod/n ratio in constantly aerated granular sludge reactors treating digester supernatant. | this study investigated how hydraulic retention time (hrt) and cod/n ratio affect nitrogen-converting consortia in constantly aerated granules treating high-ammonium digester supernatant. three hrts (10, 13, 19 h) were tested at cod/n ratios of 4.5 and 2.3. denaturing gradient gel electrophoresis and relative real-time pcr were used to characterize the microbial communities. when changes in hrt and cod/n increased nitrogen loading, the ratio of the relative abundance of aerobic to anaerobic ammo ... | 2014 | 24384323 |
| development of a deletion mutant of pseudomonas denitrificans that does not degrade 3-hydroxypropionic acid. | pseudomonas denitrificans is a gram-negative bacterium that can produce vitamin b12 under aerobic conditions. recently, recombinant strains of p. denitrificans overexpressing a vitamin b12-dependent glycerol dehydratase (dhab) were developed to produce 3-hydroxypropionic acid (3-hp) from glycerol. the recombinant p. denitrificans could produce 3-hp successfully under aerobic conditions without an exogenous supply of vitamin b12, but the 3-hp produced disappeared during extended cultivation due t ... | 2014 | 24519457 |
| improved vitamin b12 fermentation process by adding rotenone to regulate the metabolism of pseudomonas denitrificans. | our previous research had revealed that the dissolved oxygen limitation was more favorable for vitamin b12 fermentation, due to its inducement to the increased glycolytic flux in pseudomonas denitrificans. in this paper, a novel strategy was implemented to further investigate the metabolic characteristics of p. denitrificans under different oxygen supply levels, by exogenously adding rotenone (a respiratory chain inhibitor interfering with the oxygen consumption) to the fermentation broths. comp ... | 2014 | 24687557 |
| comparative genome analysis of pseudomonas knackmussii b13, the first bacterium known to degrade chloroaromatic compounds. | pseudomonas knackmussii b13 was the first strain to be isolated in 1974 that could degrade chlorinated aromatic hydrocarbons. this discovery was the prologue for subsequent characterization of numerous bacterial metabolic pathways, for genetic and biochemical studies, and which spurred ideas for pollutant bioremediation. in this study, we determined the complete genome sequence of b13 using next generation sequencing technologies and optical mapping. genome annotation indicated that b13 has a va ... | 2015 | 24803113 |
| coenzyme b12 can be produced by engineered escherichia coli under both anaerobic and aerobic conditions. | coenzyme b12 (vitamin b12 ) is one of the most complex biomolecules and an essential cofactor required for the catalytic activity of many enzymes. pseudomonas denitrificans synthesizes coenzyme b12 in an oxygen-dependent manner using a pathway encoded by more than 25 genes that are located in six different operons. escherichia coli, a robust and suitable host for metabolic engineering was used to produce coenzyme b12 . these genes were cloned into three compatible plasmids and expressed heterolo ... | 2014 | 25146562 |
| interactive performances of betaine on the metabolic processes of pseudomonas denitrificans. | the performances of betaine on the metabolic processes of vitamin b12-producing pseudomonas denitrificans were investigated in this paper. the results showed that betaine was an indispensable methyl-group donor for vitamin b12 biosynthesis, but large amounts of the extracellular glycine accompanied by betaine metabolism would impose a severe restriction on the cell growth of p. denitrificans. by further using a comparative metabolomics approach coupled with intracellular free amino acids analysi ... | 2015 | 25502424 |
| industrial vitamin b12 production by pseudomonas denitrificans using maltose syrup and corn steep liquor as the cost-effective fermentation substrates. | the aerobic pseudomonas denitrificans is widely used for industrial and commercial vitamin b12 fermentation, due to its higher productivity compared to the anaerobic vitamin b12-producing microorganisms. this paper aimed to develop a cost-effective fermentation medium for industrial vitamin b12 production by p. denitrificans in 120,000-l fermenter. it was found that maltose syrup (a low-cost syrup from corn starch by means of enzymatic or acid hydrolysis) and corn steep liquor (csl, a by-product ... | 2015 | 25561346 |
| inducible gene expression system by 3-hydroxypropionic acid. | 3-hydroxypropionic acid (3-hp) is an important platform chemical that boasts a variety of industrial applications. gene expression systems inducible by 3-hp, if available, are of great utility for optimization of the pathways of 3-hp production and excretion. | 2015 | 26500695 |
| the online morphology control and dynamic studies on improving vitamin b12 production by pseudomonas denitrificans with online capacitance and specific oxygen consumption rate. | the relationship between the morphological character of pseudomonas denitrificans and vitamin b12 synthesis based on real-time capacitance measurement and online specific oxygen consumption rate (q o2) control was established for enhancing vitamin b12 production. results demonstrated that the threshold q o2 value lower than 2.0 mmol/gdcw/l would greatly stimulate the state transfer from the cell number growth phase to the cell elongation phase and promote rapid vitamin b12 biosynthesis, while th ... | 2016 | 27022751 |
| characterisation of a 3-hydroxypropionic acid-inducible system from pseudomonas putida for orthogonal gene expression control in escherichia coli and cupriavidus necator. | 3-hydroxypropionic acid (3-hp) is an important platform chemical used as a precursor for production of added-value compounds such as acrylic acid. metabolically engineered yeast, escherichia coli, cyanobacteria and other microorganisms have been developed for the biosynthesis of 3-hp. attempts to overproduce this compound in recombinant pseudomonas denitrificans revealed that 3-hp is consumed by this microorganism using the catabolic enzymes encoded by genes hpdh, hbdh and mmsa. 3-hp-inducible s ... | 2017 | 28496205 |