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increase in swimming endurance capacity of mice by capsaicin-induced adrenal catecholamine secretion.increase in endurance swimming capacity caused by capsaicin (cap), a pungent component of red pepper, -induced increase of fat metabolism in mice was investigated using an adjustable-current water pool. the mice administered cap via a stomach tube, showed longer swimming time until exhaustion than the control group of mice, in a dose-dependent manner. the maximal effect was observed at a dose of 10 mg/kg while more than 15 mg/kg had no effect. the increase of endurance was observed only when cap ...19979362118
isolation and characterization of cytosolic copper/zinc superoxide dismutase from capsicum annuum l.a cdna clone for a cytosolic cu/zn superoxide dismutase (cu/znsod) was isolated and characterized from red pepper (capsicum annuum l.). the clone consisted of 735 bp containing one open reading frame (orf) of 459 bp, 46 bp of 5'- and 230 bp of 3'-untranslated region. the nucleotide sequence of the orf showed 93% homology with that of nicotiana plumbaginifolia and tomato. it encodes a polypeptide of 154 amino acids with a molecular weight of 15,300. genomic southern hybridization suggested that o ...19979387156
effect of curcumin and capsaicin on arachidonic acid metabolism and lysosomal enzyme secretion by rat peritoneal macrophages.the inflammatory mediators secreted by macrophages play an important role in autoimmune diseases. spice components, such as curcumin from turmeric and capsaicin from red pepper, are shown to exhibit antiinflammatory properties. the influence of these spice components on arachidonic acid metabolism and secretion of lysosomal enzymes by macrophages was investigated. rat peritoneal macrophages preincubated with 10 microm curcumin or capsaicin for 1 h inhibited the incorporation of arachidonic acid ...19979397403
food intake, energy balance and body weight control.obesity is a multifactorial and complex affectation that is characterized by a long-term excess energy intake (ei) above energy expenditure (ee). since fat oxidation seems to be dependent on sns activation and also seems to remain acutely unaffected by fat intake, this macronutrient is certainly partly responsible for this situation. in addition, high-fat intake does not induce as potent satiety signals or a compensation effect on subsequent ei as do diets rich in carbohydrates or proteins. more ...19979426360
recent advances in understanding of vanilloid receptors: a therapeutic target for treatment of pain and inflammation in skin.c-fiber sensory afferent neurons, which contain neuropeptides such as calcitonin-gene related peptide and substance p, mediate a wide variety of physiologic responses, including chemogenic pain, thermoregulation, and neurogenic inflammation. capsaicin, the pungent constituent in red pepper, functions to activate and then, at higher doses and longer times, desensitize this class of neurons. this latter response provides the basis for the therapeutic application of capsaicin. a major advance in th ...19979487017
effect of capsaicin-containing red pepper sauce suspension on upper gastrointestinal motility in healthy volunteers.afferent nerves play a major role in the regulation of gastrointestinal motility. the questions remains if specific food ingredients can selectively activate such fibers. the aim of the study was to investigate the effect of intraesophageal application of a capsaicin-containing red pepper sauce (tabasco) suspension on upper gastrointestinal motility in a controlled trial. after a baseline recording [esophageal motility, balloon distension, electrogastrogram (egg)], red pepper or saline solution ...19989635602
in vitro antimutagenic and in vivo anticlastogenic effects of carotenoids and solvent extracts from fruits and vegetables rich in carotenoids.the water insoluble residues of some carotenoid-rich fruits and vegetables, such as apricots, oranges, brussels sprouts, carrots, yellow-red peppers, and tomatoes, were sequentially extracted with n-hexane, dichloromethane, acetone, and 2-propanol, and solvent extracted materials were tested for inhibition of mutagenicities induced by aflatoxin b1 (afb1), benzo[a]pyrene (bap), 2-amino-3-methylimidazo[4,5-f]quinoline (iq), and cyclophosphamide (cp) in histidine-deficient strains of salmonella typ ...19989639691
effect of pepper and bismuth subsalicylate on gastric pain and surface hydrophobicity in the rat.the mechanism by which dietary pepper causes dyspepsia and epigastric pain is poorly understood, as is the ability of bismuth subsalicylate (bss) to relieve these symptoms.19989663730
chemoprotective properties of some pungent ingredients present in red pepper and ginger.there has been a substantial body of data, supporting that dietary factors have a profound impact on prevention as well as etiology of human cancer. capsaicin has been tested by many investigators for its effects on experimental carcinogenesis and mutagenesis. data in the literature indicate that capsaicin has dual effects on carcinogenic and mutagenic processes. at present, there is no solid evidence that hot red and chili peppers or their principal pungent ingredient capsaicin are carcinogenic ...19989675305
inhibition of listeria monocytogenes and aeromonas hydrophila by plant extracts in refrigerated cooked beef.refrigerated ready-to-eat foods are becoming increasingly popular but are often vulnerable to contamination and subsequent growth by psychrotrophic foodborne pathogens. consequently, there is a need for additional methods to assure the safety of these foods. beef slices prepared from roasted whole sirloin tips were used in the study. nine plant extracts were evaluated for ability to inhibit the growth of two psychrotrophic pathogens (aeromonas hydrophila and listeria monocytogenes) in refrigerat ...19989708301
vegetable and fruit consumption and prostate cancer risk: a cohort study in the netherlands.the association between 21 vegetables and eight fruits and prostate cancer risk was assessed in the netherlands cohort study among 58,279 men of ages 55-69 years at baseline in 1986. after 6.3 years of follow-up, 610 cases with complete vegetable data and 642 cases with complete fruit data were available for analysis. in multivariate case-cohort analyses, the following rate ratios (rrs) and 95% confidence intervals (cis) for vegetable consumption were found (comparing highest versus lowest quint ...19989718219
protective effects of fruits and vegetables against in vivo clastogenicity of cyclosphosphamide or benzo[a]pyrene in mice.seven fruits and 10 vegetables commonly consumed in germany were investigated for their anticlastogenic potencies against cyclophosphamide (cp) and benzo[a]pyrene (bap) in the in vivo mouse bone marrow micronucleus assay. we detected protective effects in 76.5% and 70.6% of the samples, respectively, and more or less distinct quantitative differences between the various plant materials and the two clastogens investigated. with respect to cp, moderate activities were exerted by sweet cherries, st ...19989734714
[is there a correlation between dietary habits and hemorrhoidal disease?].it is empirically accepted that certain foods play a role in the pathogenesis of hemorrhoids or their acute exacerbation. the aim of this work was to determine whether there is a relationship between hemorrhoids and certain food-related or common toxin-related factors.19989767961
cross-reactivity between latex and sweet pepper due to prohevein. 19989821486
lack of tumor promoting activity of capsaicin, a principal pungent ingredient of red pepper, in mouse skin carcinogenesis.capsaicin (trans-8-methyl-n-vanillyl-6-nonenamide) is the major pungent principle of hot peppers of the genus capsicum. there have been numerous investigations to evaluate the effects of capsaicin on experimental carcinogenesis and mutagenesis, but the results are discordant. in the present study, we have assessed the tumor promoting potential of capsaicin using a two stage mouse skin carcinogenesis model. repeated applications of capsaicin (10 mumol) onto the shaven backs of female icr mice fol ...19989891468
effects of red pepper added to high-fat and high-carbohydrate meals on energy metabolism and substrate utilization in japanese women.the effects of dietary red pepper added to high-fat (hf) and high-carbohydrate (hc) meals on energy metabolism were examined in thirteen japanese female subjects. after ingesting a standardized dinner on the previous evening, the subjects took an experimental breakfast (1883 kj) under the following four conditions: hf meal, hf and red-pepper (10 g) meal, hc meal, or hc and red-pepper meal. palatability of the experimental meals was measured immediately after the meals. expired air was collected ...199810211048
determinants of salt use in cooked meals in the netherlands: attitudes and practices of food preparers.to assess current habits of using salt and other seasonings in food preparation, and to investigate the psychosocial determinants of salt use. in addition we examined differences in salt consumption and psychosocial determinants with respect to stages of change and socio-economic classes.199910369495
[effect of green vegetable on digestive tract absorption of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in rats].the effect green vegetable on fecal excretion of polychlorinated dibenzo-p-dioxin (pcdd) congeners and polychlorinated dibenzofuran (pcdf) congeners was examined in male rats. the rats were administered 10% vegetable diets or a basal diet containing 0.2 ml of the causal rice-bran oil of yusho that had occurred in the southwest part of japan in 1968 and kept on the same diet for five days. the fecal excretion of 2,3,7,8-t4cdd and 2,3,4,7,8-p5cdf in the group fed with komatsuna, mitsuba, spinach a ...199910396873
modulation of intestinal functions by food substances.the small intestinal epithelium plays a crucial role in the digestion/modification of food components, absorption of nutrients and recognition of food-derived signals. it also acts as a barrier against unfavourable materials in food. these intestinal functions may be influenced by food substances. in this paper, the effects of various food substances on the intestinal functions, particularly the absorption functions, are discussed. a new assay method, using a monolayer culture system of human in ...199910399347
capsaicin induces cystatin s-like substances in submandibular saliva of the rat.irritating dietary substances such as tannin and papain have been reported to alter the morphology of salivary glands and their secretions. such alterations can be one line of protection from toxic or irritating substances in food. we investigated the effects of dietary capsaicin (a pungent ingredient of hot red pepper) on the rat submandibular gland and its secretions. several groups of animals were offered either control diets or diets containing capsaicin (from 0.0001 to 0.1%) for seven days. ...199910520965
study of lability and kinetics of the main carotenoid pigments of red pepper in the de-esterification reaction.different concentrations of potassium hydroxide in methanol were used to control the time required for complete de-esterification of the carotenoids present in paprika oleoresin, and the effectiveness of the overall process was determined. with concentrated solutions of potash, 20% and 30% (w/v), the reaction was completed in a shorter time but the overall process was less effective. solutions of low concentration, 5% and 10% (w/v), enabled the kinetic study to be carried out and the rates of de ...199810554278
change in the carotenoid and antioxidant content of spice red pepper (paprika) as a function of ripening and some technological factors.a study was conducted to investigate the change in quality attributes of red pepper (paprika) (capsicum annuum l. var. km-622) as a function of ripening and some technological factors. of quality attributes, carotenoids and bioantioxidants (ascorbic acid and tocopherols) have been studied. it was found that the dynamics of fruit ripening with regard to carotenoids and bioantioxidants was influenced to a considerable extent by weather conditions of the production season. a rainy and cool season y ...199910563856
2,4-nonadienal and benzaldehyde bioantimutagens in fushimi sweet pepper (fushimi-togarashi).fushimi sweet pepper, "fushimi-togarashi", is one of the "kyo-yasai", traditional vegetables, in kyoto, japan. the chloroform fraction of fushimi sweet pepper showed bioantimutagenicity on uv induced mutation in escherichia coli b/r wp2. the bioantimutagen was purified with silica gel chromatography and identified as 2, 4-nonadienal (id(50) = 20 microg/plate) on the basis of gc retention time and ei-ms spectrum of authentic 2,4-nonadienal. the sweet pepper also contained a known bioantimutagen, ...199910563930
effects of roasting on pyrazine contents and oxidative stability of red pepper seed oil prior to its extraction.red pepper seeds were roasted with constant stirring for 6, 9, 10, and 12 min at 210 degrees c, and oils were extracted from the roasted red pepper seeds using an expeller. the iodine values and fatty acid compositions of red pepper seed oils did not change with roasting time. the fatty acid composition of the oil obtained from the red pepper seeds roasted for 6 min was 0.24% myristic acid, 13. 42% palmitic acid, 0.33% palmitoleic acid, 2.07% stearic acid, 10. 18% oleic acid, 73.89% linoleic aci ...199910564041
latex type i sensitization and allergy in children with atopic dermatitis. evaluation of cross-reactivity to some foods.recent studies have demonstrated that allergy to natural rubber latex (nrl) is associated with cross-reactivity to certain foods. the aim of this study was to investigate the prevalence of nrl sensitization and allergy in children with atopic dermatitis (n=74). we also examined cross-reactions between latex and foods, and compared the frequency of suspected latex cross-reacting fruits in children with and without nrl-specific immunoglobulin e (ige). twelve of the 74 atopic children studied (16.2 ...199910565556
lipid peroxidation and the oxidative burst associated with infection of capsicum annuum by botrytis cinereaa combination of electron paramagnetic resonance (epr) spectroscopy and analytical chemistry has been used to study the changes in free radical content, transition metal ion status and lipid peroxidation following inoculation of fruits of sweet pepper (capsicum annuum) with botrytis cinerea. epr detected a high concentration of an unidentified free radical associated with the spreading lesion that extends into the surrounding, healthy tissues. in addition, the epr-detectable iron(iii) was highes ...199910607300
evaluation of the efficacy of the crude extracts of capsicum frutescens, citrus limon and opuntia vulgaris against newcastle disease in domestic fowl in tanzania.prophylactic and therapeutic efficacy of a combination of capsicum frutescens (red pepper), citrus limon (lemon) and opuntia vulgaris (prickly pear) against newcastle disease (nd) in domestic fowl were evaluated. eighty-eight broiler chickens were divided into five groups. birds from three groups were inoculated with velogenic nd virus strain, whereas birds from two groups were left as controls. two groups received a mixture of the plant extract three days prior to inoculation and birds from one ...199910624862
isolation and characterization of mitochondrial manganese superoxide dismutase (mnsod) from capsicum annuum l.a cdna clone for a mitochondrial manganese superoxide dismutase (mnsod) was isolated and characterized from red pepper (capsicum annuum l.). the clone consisted of 941 bp containing one open reading frame (orf) of 687 bp, 34 bp/220 bp of 5'/3'-untranslated region. amino acid sequence of the orf showed the highest homology (86%) with that of nicotiana plumbaginifolia. it encodes for a polypeptide of 228 amino acids with a molecular mass of 25.5 kda and a pi value of 8.39. genomic southern hybridi ...199910672929
nutritional quality and the presence of anti-nutritional factors in leaf protein concentrates (lpc).leaf protein concentrates were prepared from 25 different plant species. the concentrates contained acceptable levels of all essential amino acids; except methionine which was limiting with chemical scores ranging between 13.5-96%. lpcs from sesbania, nebergrass, lufa and sweet pepper were lethal to the rats. per ranged between 0.28 for safflower lpc up to 1.95 for rape. positive nitrogen balance was obtained with diets based on lpcs with mean values ranging between 6.4 and 22.42 mg n per day. t ...199910719564
effects of red pepper on appetite and energy intake.two studies were conducted to investigate the effects of red pepper (capsaicin) on feeding behaviour and energy intake. in the first study, the effects of dietary red pepper added to high-fat (hf) and high-carbohydrate (hc) meals on subsequent energy and macronutrient intakes were examined in thirteen japanese female subjects. after the ingestion of a standardized dinner on the previous evening, the subjects ate an experimental breakfast (1883 kj) of one of the following four types: (1) hf; (2) ...199910743483
[topical administration of capsaicin in dermatology for treatment of itching and pain].treatment of pruritus as observed in many dermatological and internal diseases may be very often disappointing. there are many reports describing the topical use of capsaicin, the pungent agent in red pepper. long term administration of capsaicin depletes neuropeptides in unmyelinated, polymodal c-type and small myelinated a delta-type cutaneous nerves that conduct pruritus and pain.200010789077
influence of intragastric perfusion of aqueous spice extracts on acid secretion in anesthetized albino rats.the effect of spices on gastric acid secretion is variable. their mechanism of action is also not well established.200010812814
formation of volatile compounds during heating of spice paprika (capsicum annuum) powder.spice paprika (red pepper; capsicum annuum) is the most cultivated spice worldwide and is used mainly for its color and pungency. however, current research is also focusing on the flavor as an important parameter. this paper deals with the kinetics of the formation of those volatiles that indicate a decrease in spice paprika quality due to maillard reaction, hydrolytic reactions, and oxidative degradation reactions of lipids such as fatty acids and carotenoids. spice paprika volatiles were quant ...200010888567
carotenoid biosynthesis changes in five red pepper (capsicum annuum l.) cultivars during ripening. cultivar selection for breeding.changes in the biosynthesis of individual carotenoid pigments have been investigated during fruit ripening of five cultivars of red pepper (capsicum annuum l.): mana, numex, belrubi, delfin, and negral (a chlorophyll-retaining mutant when ripe). the study was carried out throughout the ripening process, and with special emphasis on the ripe stage, to discover possible differences between cultivars and to characterize these by their carotenoid pattern and content for selecting the best varieties ...200010995282
estimation of precision and efficiency mass transfer steps for the determination of pesticides in vegetables aiming at the expression of results with reliable uncertainty.a 'bottom-up' approach for the expression of results obtained from analytical methods that include analytical steps with recovery inherently different from 100% [mass transfer steps (mts): extraction, evaporation, clean-up procedures, digestion, etc.] is presented. the estimation of the combination of all mts uncertainty involves the comparison of the experimental dispersion of replicated analyses of spiked samples with the estimation of the uncertainty obtained for the combination of all uncert ...200011002928
cdna cloning and characterization of a plant protein that may be associated with the harpinpss-mediated hypersensitive response.hypersensitive response-assisting protein (hrap) is a novel plant protein that can intensify the harpinpss-mediated hypersensitive response (hr) in harpinpss-insensitive plants, such as the vegetative stage of sweet pepper. in this report, we identified a hrap cdna clone from sweet pepper (capsicum annuum cv. ecw). the sequence of this cdna clone showed no appreciable similarity to any other known sequences. however, it contained three positively charged regions, a typical signal peptide and a c ...200011052195
effect of red peppers (capsicum frutescens) intake during gestation on thermonociceptive response of rat offspring.oral administration of aqueous red pepper (capsicum frutescens, cf) solution and low capsaicin (8-methyl-n-vanillyl-6-nonenamide) doses during gestation produces an increase in the latency of the thermonociceptive escape response of rat offspring. the present work shows that different amounts of cf (10%, 25% and 50%) incorporated to normal food of gestating rats modify in a dose-dependent manner the flexion reflex latency (r), as well as the latency of appearance of antialgesic behaviours expres ...200111165333
enzymatic hydrolysis of carotenoid fatty acid esters of red pepper (capsicum annuum l.) by a lipase from candida rugosa.analyses of red pepper extracts which had been pretreated with lipase type vii (ec 3.1.1.3.) from candida rugosa showed for the first time pepper carotenoid esters to be substrates of this enzyme. however, the extent of enzymatic hydrolysis depends on the respective carotenoid and was not quantitative compared to chemical saponification. after enzymatic cleavage, 67-89% of total capsanthin, 61-65% of total zeaxanthin, 70-81% of total beta-cryptoxanthin and 70-86% of total violaxanthin were detec ...200011204204
treatment of prurigo nodularis with topical capsaicin.prurigo nodularis is an eruption of lichenified or excoriated nodules caused by intractable pruritus that is difficult to treat. therefore the antipruritic efficacy of capsaicin seemed to be of particular interest because this alkaloid, extractable from red pepper, interferes with the perception of pruritus and pain by depletion of neuropeptides in small sensory cutaneous nerves.200111209117
dna repair effect of traditional sweet pepper fushimi-togarashi: seen in suppression of uv-induced cyclobutane pyrimidine dimer in human fibroblast.the aqueous fraction of fushimi sweet pepper increased the repair effect of the solvent control against uv-induced cyclobutane pyrimidine dimers in human fibroblast to 150%, but ordinary sweet pepper did not have a statistically significant effect. when fushimi sweet pepper was boiled, the activity of the aqueous fraction was elevated to 209% of the control (p<0.05), while that of the grilled state was decreased to 125% of the control. the repair activity of a dialyzate (mw<12,000) of the aqueou ...200011210119
inhibitory effects of curcumin and capsaicin on phorbol ester-induced activation of eukaryotic transcription factors, nf-kappab and ap-1.recently, considerable attention has been focused on identifying dietary and medicinal phytochemicals that can inhibit, retard or reverse the multi-stage carcinogenesis. spices and herbs contain phenolic substances with potent antioxidative and chemopreventive properties. curcumin, a yellow colouring agent from turmeric and capsaicin, a pungent principle of red pepper exhibit profound anticarcinogenic and antimutagenic activities. two well-defined eukaryotic transcription factors, nuclear factor ...200011216470
effects of capsaicin-containing yellow curry sauce on sympathetic nervous system activity and diet-induced thermogenesis in lean and obese young women.the present study was designed to investigate the effect of capsaicin, a pungent component of red pepper, on the sympathetic nervous system (sns) activity and energy metabolism in 16 age- and height-matched lean and obese young women. the sympatho-vagal activities were assessed by means of spectral analysis of heart rate variability (hrv) during the resting condition and after the meal (2,016 kj) with capsaicin (3 mg). energy expenditure was also measured under the two conditions. there was no s ...200011227803
combined effects of red pepper and caffeine consumption on 24 h energy balance in subjects given free access to foods.the effects of red pepper and caffeine ingestion on energy and macronutrient balances were examined in eight caucasian male subjects. all subjects participated in two randomly assigned conditions: control and experimental (red pepper and caffeine). after ingesting a standardized breakfast, subjects ate three meals ad libitum (lunch, dinner and breakfast) and snacks which were served approximately 2 h after the lunch and dinner over a 24 h period. two appetizers with or without 3 g red pepper) we ...200111242488
[forensic medical aspects of injuries inflicted with self-defense capsaicin aerosols].toxicological and forensic medical characteristics of capsaicinoids are presented. capsaicinoids represent a mixture of three basic compounds with similar structure: capsaicine, dihydrocapsaicine, and nordihydrocapsaicine, all of which are the primary components of red pepper extract (oleoresin capsicum). the advantages of this group of irritants in comparison with synthetic irritants are discussed. probable routes of administration of the extract with regard to its hazard for the organism are c ...201311255965
capsaicin-induced apoptosis in sk-hep-1 hepatocarcinoma cells involves bcl-2 downregulation and caspase-3 activation.hepatocellular carcinoma is one of the most lethal malignancies and there is no effective preventive measure in this highly malignant disease to date. in the present study, we investigated the chemopreventive potential of capsaicin (8-methyl-n- vanillyl-6-nonenamide), the principal pungent ingredient found in hot red pepper, in sk-hep-1 hepatocellular carcinoma cells. treatment of capsaicin inhibited growth of sk-hep-1 cells in a concentration-dependent manner while 4-methoxy capsaicin (met-caps ...200111275362
transgenic rice plants expressing the ferredoxin-like protein (ap1) from sweet pepper show enhanced resistance to xanthomonas oryzae pv. oryzae.we used particle bombardment to cotransform mature seed-derived rice callus (oryza sativa l., ssp. japonica, cv. eyi 105) with plasmids containing the linked marker genes gusa and hpt, and the ap1 gene encoding an amphipathic protein previously shown to delay the hypersensitive response induced in non-host plants by the pathogen pseudomonas syringae pv. syringae (pss). thirty-two independent lines of transgenic rice plants were regenerated, and 27 of these lines carried all three transgenes as s ...200111297801
a comparison of sole carbon source utilization patterns and phospholipid fatty acid profiles to detect changes in the root microflora of hydroponically grown crops.sole carbon source utilization (scsu) patterns and phospholipid fatty acid (plfa) profiles were compared with respect to their potential to characterize root-inhabiting microbial communities of hydroponically grown crops. sweet pepper (capsicum annum cv. evident), lettuce (lactuca sativa cv. grand rapids), and four different cultivars of tomato (lycopersicon esculentum cvs. gitana, armada, aromata, and elin) were grown in 1-l black plastic beakers placed in a cultivation chamber with artificial ...200111358169
food intake of patients with atopic dermatitis.there is only restricted information about the nutritional behavior of adult patients with atopic dermatitis (ad). our purpose was to evaluate the food intake in a series of patients with ad with particular consideration of self-reported food intolerance. particular attention was paid to the risks of nutrient deficiencies. we examined the intake of 28 food items in 116 ad patients with a food-frequency questionnaire (ffq). for each food item the cohort was divided in two groups according to whet ...201511358724
botrydial is produced in plant tissues infected by botrytis cinerea.the fungal metabolite botrydial was detected for the first time in ripe fruits of sweet pepper (capsicum annuum) wound-inoculated with conidial suspensions of botrytis cinerea and also in leaves of phaseolus vulgaris and arabidopsis thaliana inoculated without wounding. this phytotoxin was produced in soft rot regions of the infection. in c. annuum, the most aggressive isolate produced the highest botrydial concentrations in planta. the levels of botrydial produced by this isolate did not correl ...200111397435
capsaicin potentiates 1,25-dihydoxyvitamin d3- and all-trans retinoic acid-induced differentiation of human promyelocytic leukemia hl-60 cells.human promyelocytic leukemia hl-60 cells are differentiated into monocytic or granulocytic lineage when treated with 1,25-dihydroxyvitamin d3 [1,25-(oh)2d3] or all-trans retinoic acid, respectively. in this study, the effect of capsaicin, an active component of the red pepper of the genus capsocum, on cell differentiation was investigated in a hl-60 cell culture system. treatment of hl-60 cells with 5-30 microg/ml capsaicin for 72 h inhibited cell proliferation and induced a small increase in ce ...200111408028
enzymatic synthesis of vanillin.due to increasing interest in natural vanillin, two enzymatic routes for the synthesis of vanillin were developed. the flavoprotein vanillyl alcohol oxidase (vao) acts on a wide range of phenolic compounds and converts both creosol and vanillylamine to vanillin with high yield. the vao-mediated conversion of creosol proceeds via a two-step process in which the initially formed vanillyl alcohol is further oxidized to vanillin. catalysis is limited by the formation of an abortive complex between e ...200111409992
alpha-tocopherol content in 62 edible tropical plants.vitamin e was determined by the high-performance liquid chromatography (hplc) method. all the plants tested showed differences in their alpha-tocopherol content and the differences were significant (p < 0.05). the highest alpha-tocopherol content was in sauropus androgynus leaves (426.8 mg/kg edible portion), followed by citrus hystrix leaves (398.3 mg/kg), calamus scipronum (193.8 mg/kg), starfruit leaves averrhoa belimbi (168.3 mg/kg), red pepper capsicum annum (155.4 mg/kg), local celery apiu ...200111410015
[mycotoxins in spices].since antiquity, spices have been used for flavoring foods and beverages and for medication. they play an important role in the national economy of several of the producing, exporting and importing countries. tropical climatic conditions under which spices are grown offer a favorable environment for the fungal and mycotoxin contamination. this review summarized information from 44 reports on the occurrence of mycotoxins (aflatoxins, ochratoxins zearalenone, fumonisins, trichothecenes) and their ...200011452374
rapid spectrophotometric determination of red and yellow isochromic carotenoid fractions in paprika and red pepper oleoresins.a rapid method has been developed for the determination of the red (r) and yellow (y) isochromic carotenoid pigments fractions in paprika and oleoresins, based on uv-visible spectrophotometric measurement at two characteristic wavelengths and application of the lambert-beer law for multicomponent mixtures. the wavelengths 472 and 508 nm were selected as the most appropriate for simultaneous quantification of these fractions in the acetone extract of pigments. experimental determination of the sp ...200111513632
failure of capsaicin-containing red pepper sauce suspension to induce esophageal motility response in patients with barrett's esophagus.the physiologic importance of afferent sensory pathways in the esophageal motor functions has been recently recognised. capsaicin-sensitive sensory afferents were shown to play a role in the maintenance of mucosal integrity of the gi tract, and regulation of human esophageal motility. the aim of this study was to investigate the effect of topical application of capsaicin-containing red pepper sauce (tabasco, 25%v/v, ph:7.0) suspension on the phasic activity of the human esophagus of healthy volu ...200111595437
ch-19 sweet, a non-pungent cultivar of red pepper, increased body temperature and oxygen consumption in humans.we investigated the effect of ch-19 sweet, a non-pungent cultivar of red pepper, on body temperature and oxygen consumption in humans. ch-19 sweet was given to 11 healthy volunteers, and core body temperature, body surface temperature and oxygen consumption were measured. the control group ingested california-wandar, which contained neither capsaicin nor capsiate. the core body temperature in the ch-19 sweet group was significantly higher than that in the control group (p<0.01). the forehead tem ...200111676017
biological activity of a fruit vegetable, "anastasia green", a species of sweet pepper.russian green sweet pepper (anastasia green) was successively extracted with hexane, acetone, methanol and 70% methanol and the extracts were further separated into a total of twenty fractions by silica gel or ods column chromatographies. the biological activities of these extracts and fractions were compared. the extracts and fractions showed higher cytotoxic activity against two human oral tumor cell lines than against normal human gingival fibroblasts, suggesting their tumor-specific action. ...200111695243
determination of aflatoxins b1, b2, g1 and g2 in spices using a multifunctional column clean-up.a rapid and simple method using a multifunctional column, which contains lipophilic and charged active sites, was developed to analyse aflatoxins b1, b2, g1 and g2 in various spices, such as red pepper and nutmeg. after extraction by acetonitrile:water (9:1) and clean-up using multisep #228 column, the aflatoxins and aflatoxin-tfa derivatives are determined using lc with fluorescence detection. recoveries of each aflatoxin b1, b2, g1 and g2 spiked to red pepper, white pepper, black pepper, nutme ...200111695861
ch-19 sweet, nonpungent cultivar of red pepper, increased body temperature in mice with vanilloid receptors stimulation by capsiate.we investigated the effect of ch-19 sweet, a nonpungent cultivar of red pepper, and capsiate, a nonpungent capsaicin analog found in ch-19 sweet on body temperature in mice. the body temperature was recorded from conscious and unrestrained mice by use of a telemetry system. the body temperature in the mice administered ch-19 sweet was higher than in the mice administered california-wandar, which contains no capsiate or capsaicin. the body temperature in the mice administered capsiate was higher ...200111767210
dietary factors and gastric cancer in korea: a case-control study.to assess gastric cancer (gc) risk in relation to dietary intake in korea, a case-control study was performed. trained dietitians interviewed 136 patients diagnosed with gc, and the same number of controls were selected by matching sex, age and hospital. a significant decrease in gc risk was observed with increased intake of baiechu kimchi (prepared with salted chinese cabbage and red pepper, etc.), baiechu kimchi-stew, garlic, mushroom and soybean milk. on the contrary, a significant increase i ...200211802218
occurrence of zearalenone in korean barley and corn foods.the presence of zearalenone (zen) in korean foods such as barley, barley-based foods, corn, corn-based foods, fruits and vegetables was investigated by direct competitive enzyme-linked immunosorbent assay (dcelisa) and the liquid chromatography (lc) method. a total of 164 samples was collected from seoul, korea, in 1998 and 1999 including 30 barley, 32 barley-based foods, 18 corn, 47 corn-based foods, 19 dried red pepper, nine dried jujube, and nine dried persimmon. average recoveries of zen fro ...200211820497
genetic diversity underlying capsaicin intake in the mishima battery of mouse strains.capsaicin is the active substance responsible for the pungent sensation produced by red pepper. in order to approach the underlying genetic mechanism for preference of red pepper, we conducted a 12-h, 1-bottle intake test of capsaicin solution using both male and female animals from the mishima battery of mouse strains: 10 wild-derived inbred strains (pgn2, bfm/2, hmi, cast/ei, njl, blg2, chd, swn, kjr, msm), 1 strain derived from the so-called fancy mouse (jf1), and 3 widely used laboratory str ...200211827737
traditional production, consumption and storage of kunu--a non alcoholic cereal beverage.a survey of the production, consumption and storage of kunu was carried out. some of the information included consumption rate, processing techniques and equipment, producer's status and grains used. about 73% consume kunu daily, 26% occasionally; 1% know it is produced but may or may not be consuming it. millet (pennisetum typhoideum), sorghum (sorghum vulgare), maize (zea mays), rice (oryza sativa) and acha (digitalis exilis) were used in its production in decreasing order of preference. the g ...200211855622
red pepper and functional dyspepsia. 200211907302
[effect of fertilization on the qualities of sweet pepper in greenhouse culture].in order to study the effect of fertilization on the qualities of sweet pepper(capsicum annul l) cultured in greenhouse, the experiment was carried out by drop irrigation. the results showed that the most sensitive organ of sweet pepper for nitrate was lateral branch, in which the nitrate content was highest. the nitrate content in leaf was lower than that in lateral branch, and in fruit, the nitrate content was the lowest. high fertilization of n, p and k had no negative influence on content of ...200211962323
studies on the antimicrobial mechanisms of capsaicin using yeast dna microarray.capsaicin is a pungent element in a variety of red peppers that are widely used as food additives and considered to be an antimicrobial factor. for our tests, we used yeast dna micro-array methods to understand the mechanisms of inhibitory effects of capsaicin. the capsaicin treatment significantly induced 39 genes from approximately 6,000 genes. these induced genes were classified as multi-drug resistance transporter genes, membrane biosynthesis genes, genes encoding stress proteins, and unchar ...200212005045
analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (orac) and ferric reducing antioxidant power (frap) assays: a comparative study.a total of 927 freeze-dried vegetable samples, including 111 white cabbages, 59 carrots, 51 snap beans, 57 cauliflower, 33 white onions, 48 purple onions, 130 broccoli, 169 tomatoes, 25 beets, 88 peas, 88 spinach, 18 red peppers, and 50 green peppers, were analyzed using the oxygen radical absorption capacity (orac) and ferric reducing antioxidant capacity (frap) methods. the data show that the orac and frap values of vegetable are not only dependent on species, but also highly dependent on geog ...200212009973
ground red peppers: capsaicinoids content, scoville scores, and discrimination by an electronic nose.high-pressure liquid chromatography (hplc) was used to determine the capsaicin, dihydrocapsaicin, and total capsaicinoids levels of different ground red pepper samples obtained from local retail markets in izmir, turkey. scoville scores were determined using sensory tests. an electronic nose (en) was used to discriminate ground red pepper samples by headspace volatiles. en data were analyzed using discriminant function analysis (dfa). an overall correct classification rate of pepper varieties by ...200212009995
sensitivity to methacholine and capsaicin in patients with unclear respiratory symptoms.capsaicin, the pungent ingredient in red pepper, is known to stimulate coughing via the sensory nervous system. earlier studies showed that patients with airway symptoms induced by chemicals and strong scents cough more after inhalation of capsaicin than healthy control subjects and this has been interpreted as a hyperreactivity of airway sensory nerves. our aim was to study airway sensitivity to inhaled capsaicin and the occurrence of airway symptoms induced by strong scents in patients who und ...200212028115
the treatment of functional dyspepsia with red pepper.: to decrease the intensity of dyspeptic symptoms by impairing the visceral nociceptive c-type fibres with capsaicin, contained in red pepper powder.200212030948
on-line hplc detection of tocopherols and other antioxidants through the formation of a phosphomolybdenum complex.an on-line method to detect and quantify antioxidant species in complex extracts has been developed as a combination of conventional hplc separation and a postcolumn reaction with phosphomolybdenum reagent at acidic ph. sample analytes were chromatographed by hplc, and the postcolumn formation of a phosphate/mo(v) complex was detected at 598 nm with an on-line absorbance detector. an optimized instrumental system was set up using pure alpha-tocopherol, and it was successfully tested with complex ...200212033800
quantitation of vitamin c content in herbal juice using direct titration.vitamin c content in fresh and freeze-dried herbal juice, such as guava (psidium guajava linn.) emblic myrobolan (phyllanthus embica linn.), lemon (citrus aurantifollia swing), sweet pepper (capsicum annuum linn.) garcinia schomburgkiana pierre and passion fruit (passiflora laurifoia linn.) was determined by direct titration with iodine. the method showed excellent linearity (r2>0.99) over the concentration ranges tested (100-500% of the amount found in the juice samples), good precision (r.s.d. ...200212039626
nitrate pollution in groundwater and strategies to reduce pollution.the input-intensive rainfed tropical ecosystem, where wet season (ws) rice (oriza sativa l.)-dry season (ds) diversified high-value upland crops like vegetables predominate, has resulted in a problem of a large leakage of n into the environment, thereby polluting the water. excessive use of n fertilizer in high-value crops grown in ds is economically motivated. out of twenty water sources evaluated in a watershed with a total area of 265 ha located in magnuang, ilocos norte, philippines, twelve ...200212079116
immunosuppressive activity of capsaicinoids: capsiate derived from sweet peppers inhibits nf-kappab activation and is a potent antiinflammatory compound in vivo.capsiate and its dihydroderivatives are the major capsaicinoids of sweet pepper. these new capsaicinoids do not activate the vanilloid receptor type 1 (vr1) but they share with capsaicin (cps)some biological activities mediated in a vr1-independent fashion. in this study we show that cps and nordihydrocapsiate (cpt) inhibit early and late events in t cell activation, including cd69, cd25 and icam-1 cell surface expression, progression to the s phase of the cell cycle and proliferation in respons ...200212115659
street foods in accra, ghana: how safe are they?to investigate the microbial quality of foods sold on streets of accra and factors predisposing to their contamination.200212163918
constitutive expression of hrap gene in transgenic tobacco plant enhances resistance against virulent bacterial pathogens by induction of a hypersensitive response.hypersensitive response-assisting protein (hrap) has been previously reported as an amphipathic plant protein isolated from sweet pepper that intensifies the harpin(pss)-mediated hypersensitive response (hr). the hrap gene has no appreciable similarity to any other known sequences, and its activity can be rapidly induced by incompatible pathogen infection. to assess the function of the hrap gene in plant disease resistance, the camv 35s promoter was used to express sweet pepper hrap in transgeni ...200212182333
arsenic, sb and bi contamination of soils, plants, waters and sediments in the vicinity of the dalsung cu-w mine in korea.the objective of this study is to investigate environmental contamination derived from metalliferous mining activities. in the study area, the dalsung cu-w mine, soils, various crop plants, stream waters, sediments and particulates were sampled in and around the mine and analyzed for as, sb and bi by icp-aes with a hydride generator. in addition, soil ph, cation exchange capacity, loss-on-ignition and soil texture were also measured. concentrations of as, sb and bi in surface soils sampled in th ...200212186294
biosynthesis of terpenes: studies on 1-hydroxy-2-methyl-2-(e)-butenyl 4-diphosphate reductase.earlier in vivo studies showed the involvement of isph protein in the conversion of 1-hydroxy-2-methyl-2-(e)-butenyl 4-diphosphate into isopentenyl diphosphate (ipp) and dimethylallyl diphosphate (dmapp). we have demonstrated now that cell extract of an escherichia coli strain engineered for hyperexpression of the isph (lytb) gene catalyzes the in vitro conversion of 1-hydroxy-2-methyl-2-(e)-butenyl 4-diphosphate into ipp and dmapp. the reaction requires nadh, fad, divalent cations (preferably c ...200212198182
suppression of phorbol ester-induced nf-kappab activation by capsaicin in cultured human promyelocytic leukemia cells.capsaicin, a major pungent constituent of red pepper (capsicum annuum l.) possesses a vast variety of pharmacologic and physiologic activities. despite its irritant properties, the compound exerts anti-inflammatory and anti-nociceptive effects. previous studies from this laboratory revealed that capsaicin, when topically applied onto dorsal skin of female icr mice, strongly attenuated activation of nf-kappab and ap-1 induced by the typical tumor promoter, 12-o-tetradecanoylphorbol-13-acetate (tp ...200212214859
chlorophyll breakdown in senescent chloroplasts (cleavage of pheophorbide a in two enzymic steps).the cleavage of pheophorbide (pheide) a into primary fluoescent chlorophyll (chl) catabolites (pfccs) in senescent chloroplasts was investigated. chloroplast preparations isolated from senescent canola (brassica napus) cotyledons exhibited light-dependent production of pfcc when assay mixtures were supplemented with ferredoxin (fd). pfcc production in detergent-solubilized membranes was dependent on the presence of an fd-reducing system. pheide a cleavage required the action of two proteins, phe ...199712223835
partial purification and characterization of red chlorophyll catabolite reductase, a stroma protein involved in chlorophyll breakdown.red chlorophyll (chl) catabolite (rcc) reductase, which catalyzes the reaction of an intermediary chl catabolite (rcc) in the two-step cleavage reaction of pheophorbide (pheide) a into primary fluorescent catabolites (pfccs) during chl breakdown, was characterized and partially purified. rcc reductase activity was present at all stages of barley leaf development and even in roots. the highest specific activity was found in senescent leaves, which were used to purify rcc reductase 1000-fold. amon ...199712223836
field and laboratory selection of frankliniella occidentalis (pergande) for resistance to insecticides.response of western flower thrips, frankliniella occidentalis (pergande), to selection for resistance to insecticides commonly used to control this pest in murcia (south-east spain) was studied under field and laboratory conditions. in the field, plots within sweet pepper crops in commercial and experimental greenhouses were treated under different selection strategies: insecticide rotation versus formetanate reiteration, formetanate reiteration versus acrinathrin reiteration, and formetanate re ...200212233182
insecticide resistance in field populations of frankliniella occidentalis (pergande) in murcia (south-east spain).thirty-nine field populations of frankliniella occidentalis (pergande) were collected from different crops (sweet pepper, tomato, lettuce, artichoke, melon, cucumber, carnation, broad bean, peach and plum) in murcia (south-east spain). all populations were reared separately in the laboratory to obtain enough individuals for bioassays. female thrips were bioassayed, using a standard topical application method, against methiocarb, methamidophos, acrinathrin, endosulfan, deltamethrin and formetanat ...200212233190
expression of a patatin-like protein in the anthers of potato and sweet pepper flowers.patatin, the major glycoprotein in potato tubers, is encoded by a multigene family. rna and protein analyses reveal that a homologous mrna and an immunologically cross-reacting protein can be found in potato flowers, which is similar to patatin in that it displays a lipid acyl hydrolase activity. the patatin-like protein found in flowers has a higher molecular weight than the authentic tuber patatin. deglycosylation experiments show that this is not due to differences in the glycosylation patter ...198912359901
a modified hplc method for the determination of ochratoxin a by fluorescence detection.a high-performance liquid chromatographic method (hplc) with fluorescent detector is described for the determination of ochratoxin a (ota). a mobile phase consisting of acetonitrile:water:acetic acid (99:99:2, v/v/v) was used for the resolution of the compound on a c(18) hypersil column. the retention time for ota and diflunisal which was used as an internal standard (is) were 11.7 and 12.8 min, respectively. the method is selective, reliable, reproducable with relative standard deviation (rsd) ...200212378560
mechanism of potent antiperoxidative effect of capsaicin.the effect of a pungent ingredient of red pepper, capsaicin, on lipid peroxidation of rat liver mitochondria (rlm) induced by adp/fe(2+) was studied. capsaicin inhibited the lipid peroxidation significantly, being more effective than the well-known antioxidant alpha-tocopherol. capsaicin was also found to scavenge 1,1'-diphenyl-2-picrylhydrazyl (dpph) radicals both in solution and in membranes, especially the latter. capsaicin was found to scavenge radicals both at/near the membrane surface and ...200212383946
antioxidant and antiproliferative activities of common vegetables.epidemiological studies have shown that consumption of fruits and vegetables is associated with reduced risk of chronic diseases. increased consumption of fruits and vegetables containing high levels of phytochemicals has been recommended to prevent chronic diseases related to oxidative stress in the human body. in this study, 10 common vegetables were selected on the basis of consumption per capita data in the united states. a more complete profile of phenolic distributions, including both free ...200212405796
effect of ascorbic acid on the stability of beta-carotene and capsanthin in paprika (capsicum annuum) powder.the effect of ascorbic acid, light, and storage on the stability of the pigments beta-carotene and capsanthin in red pepper (capsicum annuum) powder has been elucidated by determining the amount of pigment in samples treated by various concentrations of ascorbic acid. determination of pigment concentration has been performed after different storage times using high-performance liquid chromatography. the dependence of the concentration of pigments on the concentration of ascorbic acid, presence o ...200212428443
[rapid analysis of aflatoxins in raw peanuts, corn, buckwheat and red pepper by a new mini-column cleanup and hplc using post-column photochemical derivatization system].a rapid and clean method for the analysis of aflatoxins (afs) was developed by using a new column and post-column photochemical derivatization hplc with fluorescence detection. the new cleanup column consisted of magnesia and basic alumina poured on the top of a commercial multi-functional mini-column. it was extremely effective for the cleanup of afs from raw peanut, corn, buckwheat and red pepper. fluorescent substances, which interfered with the analysis of afs from corn, were completely abso ...200212436711
roles of jnk-1 and p38 in selective induction of apoptosis by capsaicin in ras-transformed human breast epithelial cells.efforts have been made to develop a chemoprevention strategy that selectively triggers apoptosis in malignant cancer cells. previous studies showed that capsaicin, the major pungent ingredient of red pepper, had differential effect between normal and transformed cells. as an approach to unveil the molecular mechanism by which capsaicin selectively induces apoptosis in transformed cells, we investigated the effect of capsaicin in nontransformed and ras-transformed cells of a common origin: parent ...200312478662
capsaicin inhibits growth of adult t-cell leukemia cells.human t-cell leukemia virus type 1 (htlv-1)-associated adult t-cell leukemia (atl) is resistant to conventional chemotherapy. we examined the in vitro effects of capsaicin, the principal ingredients of red pepper, on three atl cell lines. capsaicin treatment inhibited the growth of atl cells both in dose- and time-dependent manner. the inhibitory effect was mainly due to the induction of cell cycle arrest and apoptosis. capsaicin treatment also induced the degradation of tax and up-regulation of ...200312537981
modifying children's food preferences: the effects of exposure and reward on acceptance of an unfamiliar vegetable.the aim of this study was to evaluate two interventions (one reward-based and one exposure-based) for increasing children's acceptance of an unfamiliar vegetable compared with a no-treatment control. it was predicted that the exposure condition would increase liking for, and consumption of, the vegetable relative to either the reward or control group.200312571670
nondestructive ftir monitoring of leaf senescence and elicitin-induced changes in plant leaves.the applicability of the ftir attenuated total reflectance technique for in situ monitoring of plant physiological processes such as leaf senescence and aging has been examined. difference spectra obtained by subtracting the spectrum of the young plant leaf from that of the older one revealed positive bands at 1650-1500 cm(-1), indicating a higher relative concentration of phenolics in the older leaves of both black cherry and sweet pepper bush leaves. prolonged physiological stress of tobacco l ...200312583010
factors influencing texture retention of salt-free, acidified, red bell peppers during storage.red bell peppers were stored in a salt-free, acidified cover solution with sulfite as a microbial preservative. the texture retention of stored peppers was evaluated as a function of ph, acid, calcium, blanch treatment, and growing conditions of the peppers. field-grown peppers softened at a faster rate than greenhouse-grown peppers and exhibited more variability in their ability to maintain texture during storage. improved firmness retention of red peppers stored at 30 degrees c was observed wh ...200312590498
molecular characterization of three 3-hydroxy-3-methylglutaryl-coa reductase genes including pathogen-induced hmg2 from pepper (capsicum annuum).sesquiterpene phytoalexins, a class of plant defense metabolites, are synthesized from the cytosolic acetate/mevalonate pathway in isoprenoids biosynthetic system of plants. the 3-hydroxy-3-methylglutaryl-coa reductase (hmgr) catalyzes the synthesis of mevalonate, which is the specific precursor of this pathway, as a multi gene family. three kinds of cdna clones encoding hmgr were isolated from korean red pepper (capsicum annuum l. cv. nockwang) and the hmgr2 gene (hmg2) was especially obtained ...200312591612
non-pungent capsaicinoids from sweet pepper synthesis and evaluation of the chemopreventive and anticancer potential.capsiate, the non-pungent ester isoster of capsaicin, and its dihydroderivative are the major capsaicinoids of sweet peppers. the remarkable difference between the sensory properties of capsaicin vs capsiate is solely due to the way the vanillyl and the acyl moieties of this basic structural motif are linked, via an amide bond in capsaicin-type compounds and via an ester bond in capsiate-type compounds.200312594536
evaluating companion planting and non-host masking odors for protecting roses from the japanese beetle (coleoptera: scarabaeidae).effectiveness of companion planting, and use of nonhost masking odors were evaluated under field conditions for protecting roses against the japanese beetle, popillia japonica newman. three reputedly effective companion species, rue (ruta graveolens l.), zonal geranium (pelargonium x hortorum bailey), and garlic chives (allium scheonparum l.) were interplanted with roses in replicated garden plots. numbers of beetles on these roses were compared with rose-only control plots on 6 d during beetle ...200312650348
[a food poisoning outbreak caused by purple washington clam contaminated with norovirus (norwalk-like virus) and hepatitis a virus].a party of 57 people dined together in a restaurant in hamamatsu city on december 11, 2001. the next day, 22 of them developed symptoms of acute gastroenteritis, such as diarrhea, vomiting, and fever. examination of 4 fecal specimens from these patients by elisa for norovirus (norwalk-like virus, nv) detected both genogroup i (gi) and genogroup ii (gii) nv in all the 4 specimens. in addition, rt-pcr and real-time pcr methods for nv detected the nv gene. approximately one month after the outbreak ...200312661084
quantification of carotenoids in chicken plasma after feeding free or esterified lutein and capsanthin using high-performance liquid chromatography and liquid chromatography-mass spectrometry analysis.the present study reports about the plasma carotenoid levels of chickens achieved after feeding diets containing free and esterified lutein (from marigold) and capsanthin (from red pepper) by applying hplc analyses using a rp-c30 column. forty chickens were divided at random into four groups and were fed 2 wk with different diets after receiving a basal diet with a low carotenoid content for carotenoid depletion (3 wk). one group was fed a diet containing free lutein (g1), another group received ...200312705399
sweet pepper ferredoxin-like protein ( pflp) gene as a novel selection marker for orchid transformation.a novel method for selection of transgenic plants utilizing the sweet pepper ( capsicum annuum l.) ferredoxin-like protein ( pflp) gene as selection marker and erwinia carotovora as the selection agent has been developed. an expression vector containing a pflp cdna driven by a cauliflower mosaic virus 35s promoter was successfully transformed into protocorm-like bodies of oncidium orchid by agrobacterium tumefaciens and particle bombardment, respectively. erwinia carotovora was used as a selecti ...200312721849
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