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mucosal and salivary microbiota associated with recurrent aphthous stomatitis.recurrent aphthous stomatitis (ras) is a common oral mucosal disorder of unclear etiopathogenesis. although recent studies of the oral microbiota by high-throughput sequencing of 16s rrna genes have suggested that imbalances in the oral microbiota may contribute to the etiopathogenesis of ras, no specific bacterial species associated with ras have been identified. the present study aimed to characterize the microbiota in the oral mucosa and saliva of ras patients in comparison with control subje ...201627036492
antimicrobial resistance and resistance genes in aerobic bacteria isolated from pork at slaughter.the aim of this study was to investigate the phenotypic and genotypic antimicrobial resistance, integrons, and transferability of resistance markers in 243 aerobic bacteria recovered from pork at slaughter in the people's republic of china. the organisms belonged to 22 genera of gram-negative bacteria (92.2%) and gram-positive bacteria (7.8%). high levels of resistance were detected to tetracycline, trimethoprim-sulfamethoxazole, and ampicillin (36.2 to 54.3%), and lower levels were detected to ...201627052863
genome-sequence analysis of acinetobacter johnsonii mb44 reveals potential nematode-virulent factors.acinetobacter johnsonii is generally recognized as a nonpathogenic bacterium although it is often found in hospital environments. however, a newly identified isolate of this species from a frost-plant-tissue sample, namely, a. johnsonii mb44, showed significant nematicidal activity against the model organism caenorhabditis elegans. to expand our understanding of this bacterial species, we generated a draft genome sequence of mb44 and analyzed its genomic features related to nematicidal attribute ...201627429894
characterisation of class 3 integrons with oxacillinase gene cassettes in hospital sewage and sludge samples from france and luxembourg.in this study, antibiotic resistance class 3 integrons in gram-negative bacteria isolated from hospital sewage and sludge and their genetic contents were characterised. two samples of hospital effluent from france and luxembourg and one sample of sludge from a wastewater treatment plant in france were collected in 2010 and 2011. bacteria were cultured on selective agar plates and integrons were detected in colonies by quantitative pcr. integron gene cassette arrays and their genetic environments ...201627499434
the genetic analysis of an acinetobacter johnsonii clinical strain evidenced the presence of horizontal genetic transfer.acinetobacter johnsonii rarely causes human infections. while most a. johnsonii isolates are susceptible to virtually all antibiotics, strains harboring a variety of β-lactamases have recently been described. an a. johnsonii aj2199 clinical strain recovered from a hospital in buenos aires produces per-2 and oxa-58. we decided to delve into its genome by obtaining the whole genome sequence of the aj2199 strain. genome comparison studies on aj2199 revealed 240 unique genes and a close relation to ...201627548264
consumption of a high-fat diet alters the seminal fluid and gut microbiomes in male mice.our prior work showed that a novel microbiome resides in the seminal vesicles of wild-type and oestrogen receptor α (esr1) knock-out mice and is impacted by the presence of functional esr1 genes. the seminal fluid microbiome (sfm) may influence the health and reproductive status of the male, along with that of his partner and offspring. a high-fat diet (hfd) alters metabolites and other factors within seminal fluid and might affect the sfm. adult (~15 weeks old) male mice were placed for 4 weeks ...201627569192
the influence of the autochthonous wastewater microbiota and gene host on the fate of invasive antibiotic resistance genes.the aim of this study was to assess the fate of invasive antibiotic resistance genes (arg) discharged in wastewater. with this objective, antibiotic resistant bacteria (arb) known to harbor specific arg were inoculated in wastewater (hospital effluent, or municipal raw and treated wastewater) and in ultra-pure sterile water microcosms. two sets of wastewater arb isolates were used - set 1, enterococcus faecalis, acinetobacter johnsonii, klebsiella pneumoniae and set 2, enterococcus faecium, acin ...201727697350
induction of diverse bioactive secondary metabolites from the mangrove endophytic fungus trichoderma sp. (strain 307) by co-cultivation with acinetobacter johnsonii (strain b2).two new sesquiterpenes, microsphaeropsisin b (1) and c (2), and two new de-o-methyllasiodiplodins, (3r, 7r)-7-hydroxy-de-o-methyllasiodiplodin (4) and (3r)-5-oxo-de-o-methyllasiodiplodin (5), together with one new natural product (6) and twelve known compounds (3, 7-17), were isolated from the co-cultivation of mangrove endophytic fungus trichoderma sp. 307 and aquatic pathogenic bacterium acinetobacter johnsonii b2. their structures, including absolute configurations, were elucidated by extensi ...201728208607
lettuce and fruits as a source of multidrug resistant acinetobacter spp.the role of ready-to-eat products as a reservoir of pathogenic species of acinetobacter remains unclear. the objective of the present study was to evaluate the presence of acinetobacter species in lettuces and fruits marketed in portugal, and their susceptibility to antimicrobials. acinetobacter spp. were isolated from 77.9% of the samples and these microorganisms were also found as endophytes (i.e. present within the plant tissue) in 12 of 20 samples of lettuces analysed. among 253 isolates tha ...201728213015
isolation and characterization of a virulent bacteriophage infecting acinetobacter johnsonii from activated sludge.a double-stranded dna phage named ajo1, infecting acinetobacter johnsonii, which plays an important role in wastewater treatment, was isolated from activated sludge in a full-scale municipal wastewater treatment plant. based on morphological taxonomy, ajo1, with an icosahedral head 55 ± 2 nm in diameter and a non-contractile tail 8 ± 2 nm in length, was classified as a member of the podoviridae family. bacterial infection characteristics were as follows: no polyvalent infectivity, optimal multip ...201728213104
a topical hydrogel with deferiprone and gallium-protoporphyrin targets bacterial iron metabolism and has antibiofilm activity.many infectious diseases are associated with multidrug-resistant (mdr) bacteria residing in biofilms that require high antibiotic concentrations. while oral drug delivery is frequently ineffective, topical treatments have the potential to deliver higher drug concentrations to the infection site while reducing systemic side effects. this study determined the antibiofilm activity of a surgical wound gel loaded with the iron chelator deferiprone (def) and the heme analogue gallium-protoporphyrin (g ...201728396543
determination of spoilage microbiota of pacific white shrimp during ambient and cold storage using next-generation sequencing and culture-dependent method.this study was conducted to determine the initial and spoilage microbiota of pacific white shrimp during ambient and cold storage using next-generation sequencing (ngs) and a culture-dependent method. the quality changes were also evaluated by sensory analysis and total volatile basic nitrogen (tvb-n) values. after 1 d of storage, the psychrotrophic bacteria were only 5.97 log cfu/g, accounting for 1.1% of the mesophilic bacteria counts (7.94 log cfu/g). the psychrotrophic bacteria counts exceed ...201728399332
mosquito microbiome dynamics, a background for prevalence and seasonality of west nile virus.symbiotic microbial communities augment host phenotype, including defense against pathogen carriage and infection. we sampled the microbial communities in 11 adult mosquito host species from six regions in southern ontario, canada over 3 years. of the factors examined, we found that mosquito species was the largest driver of the microbiota, with remarkable phylosymbiosis between host and microbiota. seasonal shifts of the microbiome were consistently repeated over the 3-year period, while region ...201728421042
draft genome sequence of acinetobacter johnsonii c6, an environmental isolate engaging in interspecific metabolic interactions.acinetobacter johnsonii c6 originates from creosote-polluted groundwater and performs ecological and evolutionary interactions with pseudomonas putida in biofilms. the draft genome of a. johnsonii c6 is 3.7 mbp and was shaped by mobile genetic elements. it reveals genes facilitating the biodegradation of aromatic hydrocarbons and resistance to antimicrobials and metals.201728428290
using phenotype microarrays in the assessment of the antibiotic susceptibility profile of bacteria isolated from wastewater in on-site treatment facilities.the scope of the study was to apply phenotype biolog microarray (pm) technology to test the antibiotic sensitivity of the bacterial strains isolated from on-site wastewater treatment facilities. in the first step of the study, the percentage values of resistant bacteria from total heterotrophic bacteria growing on solid media supplemented with various antibiotics were determined. in the untreated wastewater, the average shares of kanamycin-, streptomycin-, and tetracycline-resistant bacteria wer ...201728451946
rna-based amplicon sequencing reveals microbiota development during ripening of artisanal versus industrial lard d'arnad.valle d'aosta lard d'arnad is a protected designation of origin (pdo) product produced from fat of the shoulder and back of heavy pigs. its manufacturing process can be very diverse, especially regarding the maturation temperature and the nacl concentration used for the brine; thereby, the main goal of this study was to investigate the impact of those parameters on the microbiota developed during curing and ripening. three farms producing lard d'arnad were selected. two plants, reflecting the in ...201728600315
evaluation of bacterial pathogen diversity, abundance and health risks in urban recreational water by amplicon next-generation sequencing and quantitative pcr.the microbial quality of urban recreational water is of great concern to public health. the monitoring of indicator organisms and several pathogens alone is not sufficient to accurately and comprehensively identify microbial risks. to assess the levels of bacterial pathogens and health risks in urban recreational water, we analyzed pathogen diversity and quantified four pathogens in 46 water samples collected from waterbodies in beijing olympic forest park in one year. the pathogen diversity rev ...201728647233
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