Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| control of diatraea saccharalis by the endophytic pantoea agglomerans 33.1 expressing cry1ac7. | despite the fact that bacillus thuringiensis (bt) is found in more than 90 % of the products used against insects, it has some difficulty reaching the internal regions where the larvae feed. to solve this problem, many genetically modified microorganisms that colonize the same pests have been developed. thus, the endophytic bacterium pantoea agglomerans (33.1), which has been recently described as a promising sugarcane growth promoter, was genetically modified with the pjtt vector (which carries ... | 2014 | 24531524 |
| the sugarcane defense protein sugarwin2 causes cell death in colletotrichum falcatum but not in non-pathogenic fungi. | plants respond to pathogens and insect attacks by inducing and accumulating a large set of defense-related proteins. two homologues of a barley wound-inducible protein (barwin) have been characterized in sugarcane, sugarwin1 and sugarwin2 (sugarcane wound-inducible proteins). induction of sugarwins occurs in response to diatraea saccharalis damage but not to pathogen infection. in addition, the protein itself does not show any effect on insect development; instead, it has antimicrobial activitie ... | 2014 | 24608349 |
| managing the sugarcane borer, diatraea saccharalis, and corn earworm, helicoverpa zea, using bt corn and insecticide treatments. | the sugarcane borer, diatraea saccharalis (fabricius) (lepidoptera: crambidae) and the corn earworm, helicoverpa zea (boddie) (lepidoptera: noctuidae), are important pests of corn in brazil and have not been successfully managed, because of the difficulty of managing them with pesticides. the objective of this study was to evaluate the effect of bt corn mon810, transformed with a gene from bacillus thuringiensis berliner (bacillales: bacillaceae) insecticide seed treatment, and foliar insecticid ... | 2013 | 24735131 |
| baseline susceptibility and monitoring of brazilian populations of spodoptera frugiperda (lepidoptera: noctuidae) and diatraea saccharalis (lepidoptera: crambidae) to vip3aa20 insecticidal protein. | the genetically modified maize expressing vip3aa20 insecticidal protein from bacillus thuringiensis berliner is abiotechnological option for the control of spodoptera frugiperda (j.e. smith) and diatraea saccharalis (f.) in brazil. to support an insect resistance management program, we conducted studies of baseline susceptibility and monitoring of brazilian populations of s. frugiperda and d. saccharalis to the vip3aa20 insecticidal protein. neonates were exposed to vip3aa20 applied on artificia ... | 2014 | 24772561 |
| defense response in non-genomic model species: methyl jasmonate exposure reveals the passion fruit leaves' ability to assemble a cocktail of functionally diversified kunitz-type trypsin inhibitors and recruit two of them against papain. | multiplicity of protease inhibitors induced by predators may increase the understanding of a plant's intelligent behavior toward environmental challenges. information about defense mechanisms of non-genomic model plant passion fruit (passiflora edulis sims) in response to predator attack is still limited. here, via biochemical approaches, we showed its flexibility to build-up a broad repertoire of potent kunitz-type trypsin inhibitors (ktis) in response to methyl jasmonate. seven inhibitors (20- ... | 2014 | 24849173 |
| modeling evolution of resistance of sugarcane borer (lepidoptera: crambidae) to transgenic bt corn. | diatraea saccharalis (f.) (lepidoptera: crambidae) is a target pest of transgenic corn expressing bacillus thuringiensis (bt) protein, and the first evidence of resistance by d. saccharalis to cry1ab corn was detected in a field population in northeast louisiana in 2004. we used a model of population dynamics and genetics of d. saccharalis to 1) study the effect of interfield dispersal, the first date that larvae enter diapause for overwintering, toxin mortality, the proportion of non-bt corn in ... | 2014 | 24914780 |
| the parasitoid, cotesia flavipes (cameron) (hymenoptera: braconidae), influences food consumption and utilization by larval diatraea saccharalis (f.) (lepidoptera: crambidae). | parasitoids exploit host insects for food and other resources; they alter host development and physiology to optimize conditions to favor parasitoid development. parasitoids influence their hosts by injecting eggs, along with a variety of substances, including venoms, polydnaviruses, ovarian fluids, and other maternal factors, into hosts. these factors induce profound changes in hosts, such as behavior, metabolism, endocrine events, and immune defense. because endoparasitoids develop and consume ... | 2014 | 25042594 |
| synergism and antagonism between bacillus thuringiensis vip3a and cry1 proteins in heliothis virescens, diatraea saccharalis and spodoptera frugiperda. | second generation bt crops (insect resistant crops carrying bacillus thuringiensis genes) combine more than one gene that codes for insecticidal proteins in the same plant to provide better control of agricultural pests. some of the new combinations involve co-expression of cry and vip genes. because cry and vip proteins have different midgut targets and possibly different mechanisms of toxicity, it is important to evaluate possible synergistic or antagonistic interactions between these two clas ... | 2014 | 25275646 |
| critical electrolyte concentration of silk gland chromatin of the sugarcane borer diatraea saccharalis, induced using agrochemicals. | the sugarcane borer diatraea saccharalis is widely known as the main pest of sugarcane crop, causing increased damage to the entire fields. measures to control this pest involve the use of chemicals and biological control with cotesia flavipes wasps. in this study, we evaluated the insecticides fipronil (frontline; 0.0025%), malathion (malatol bio carb; 0.4%), cipermetrina (galgotrin; 10%), and neem oil (natuneem; 100%) and the herbicide nicosulfuron (sanson 40 sc; 100%) in the posterior region ... | 2014 | 25299111 |
| geographic population structure of the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), in the southern united states. | the sugarcane borer moth, diatraea saccharalis, is widespread throughout the western hemisphere, and is considered an introduced species in the southern united states. although this moth has a wide distribution and is a pest of many crop plants including sugarcane, corn, sorghum and rice, it is considered one species. the objective was to investigate whether more than one introduction of d. saccharalis had occurred in the southern united states and whether any cryptic species were present. we fi ... | 2014 | 25337705 |
| binding analysis of bacillus thuringiensis cry1 proteins in the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | sugarcane borer (diatraea saccharalis, f.) is an important corn pest in south america and united states. the aim of the present study was to analyze the susceptibility and binding interactions of three cry1a proteins and cry1fa in a brazilian d. saccharalis population. the results showed that cry1ab was the most active, followed by cry1ac, cry1fa and cry1aa. all cry1-biotinylated proteins tested bound specifically to the d. saccharalis brush border membrane vesicles (bbmv). heterologous competit ... | 2015 | 25736726 |
| isolation of cellulolytic bacteria from the intestine of diatraea saccharalis larvae and evaluation of their capacity to degrade sugarcane biomass. | as a strategy to find efficient lignocellulose degrading enzymes/microorganisms for sugarcane biomass pretreatment purposes, 118 culturable bacterial strains were isolated from intestines of sugarcane-fed larvae of the moth diatraea saccharalis. all strains were tested for cellulolytic activity using soluble carboxymethyl cellulose (cmc) degrading assays or by growing bacteria on sugarcane biomass as sole carbon sources. out of the 118 strains isolated thirty eight were found to possess cellulos ... | 2015 | 25852992 |
| impacts of nosema sp. (microsporidia: nosematidae) on the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | in brazil, the sugarcane borer, diatraea saccharalis (fabricius, 1794) (lepidoptera: crambidae), is controlled with massive releases of the hymenopteran parasitoid cotesia flavipes cam. (hymenoptera: braconidae); over 3 million hectares of sugarcane are treated annually with 18 billion parasitoids. in order to meet this demand, parasitoids are produced in d. saccharalis under laboratory conditions where a nosema sp. is reported to be an important problem in mass rearing of the host. the goals fo ... | 2015 | 25981134 |
| differential expression of the pr1a gene in metarhizium anisopliae and metarhizium acridum across different culture conditions and during pathogenesis. | the entomopathogenic fungi of the genus metarhizium have several subtilisin-like proteases that are involved in pathogenesis and these have been used to investigate genes that are differentially expressed in response to different growth conditions. the identification and characterization of these proteases can provide insight into how the fungus is capable of infecting a wide variety of insects and adapt to different substrates. in addition, the pr1a gene has been used for the genetic improvemen ... | 2015 | 25983629 |
| identification, inheritance, and fitness costs of cry2ab2 resistance in a field-derived population of sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae). | the sugarcane borer, diatraea saccharalis (f.), is one of the major target pests of transgenic maize, zea mays, expressing bacillus thuringiensis (bt) proteins in south america and mid-southern region of the u.s. the mon89034 maize expresses cry2ab2 and cry1a.105 bt proteins and it was developed to provide better control of key lepidopteran pests of maize including d. saccharalis. the objectives of this study were to select and characterize the resistance of d. saccharalis to cry2ab2 using a non ... | 2015 | 26205174 |
| testis morphology and dichotomic spermatogenesis in the sugarcane borer diatraea saccharalis (f.) (lepidoptera: crambidae). | in the present study, we describe the testis morphology and spermatogenesis of the sugarcane borer, one of the most destructive pests of sugarcane, using light, transmission, and scanning electron microscopies. diatraea saccharalis (f.) larvae have paired, kidney-shaped testes that are limited externally and divided into four follicles by tunica cells. the adults have two fused whitish and spherical testes that are divided into eight follicles and are composed of spermatogonia, spermatocyte, spe ... | 2015 | 26245268 |
| bowman-birk proteinase inhibitor from clitoria fairchildiana seeds: isolation, biochemical properties and insecticidal potential. | herein described is the biochemical characterisation, including in vitro and in vivo assays, for a proteinase inhibitor purified from clitoria fairchildiana seeds (cfpi). purification was performed by hydrophobic interaction and gel filtration chromatography. kinetic studies of the purified inhibitor showed a competitive-type inhibitory activity against bovine trypsin and chymotrypsin, with an inhibition stoichiometry of 1:1 for both enzymes. the inhibition constants against trypsin and chymotry ... | 2015 | 26330217 |
| lepidoptera (crambidae, noctuidae, and pyralidae) injury to corn containing single and pyramided bt traits, and blended or block refuge, in the southern united states. | fall armyworm, spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae); corn earworm, helicoverpa zea boddie (lepidoptera: noctuidae); southwestern corn borer, diatraea grandiosella dyar (lepidoptera: crambidae); sugarcane borer, diatraea saccharalis f. (lepidoptera: crambidae); and lesser cornstalk borer, elasmopalpus lignosellus zeller (lepidoptera: pyralidae), are lepidopteran pests of corn, zea mays l., in the southern united states. blended refuge for transgenic plants expressing the i ... | 2015 | 26470116 |
| genetic diversity and structure of brazilian populations of diatraea saccharalis (lepidoptera: crambidae): implications for pest management. | the sugarcane borer, diatraea saccharalis (f.), is the main pest of sugarcane in brazil. genetic variability and gene flow among 13 brazilian populations of the species were evaluated based on mitochondrial dna sequences to estimate the exchange of genetic information within and among populations. we found high genetic structure among sampled localities (φst=0.50923), and pairwise genetic distances were significantly correlated to geographic distances. demographic analysis and genealogical netwo ... | 2015 | 26470135 |
| development of a new container for storage and release of the parasitoid cotesia flavipes (hymenoptera: braconidae). | in brazil, the parasitoid cotesia flavipes (cameron) has been used as a biological control agent against diatraea saccharalis (f.) since 1976, because of its effectiveness in reducing populations of this pest. three million plastic cups are used annually to release c. flavipes, which remain in the environment. this study aimed to develop fully biodegradable packaging, so that releases occur without damaging the environment. the biological characteristics of c. flavipes were studied for five gene ... | 2015 | 26470218 |
| resistance risk assessment of spodoptera frugiperda (lepidoptera: noctuidae) and diatraea saccharalis (lepidoptera: crambidae) to vip3aa20 insecticidal protein expressed in corn. | transgenic agrisure viptera 3 corn that expresses cry1ab, vip3aa20, and epsps proteins and agrisure viptera expressing vip3aa20 are used for control of spodoptera frugiperda (j.e. smith) and diatraea saccharalis (f.) in brazil. to support a resistance management program, resistance risk assessment studies were conducted to characterize the dose expression of vip3aa20 protein and level of control against these species. the vip3aa20 expression in agrisure viptera 3 and agrisure viptera decreased f ... | 2015 | 26470366 |
| cytotoxic effects of neem oil in the midgut of the predator ceraeochrysa claveri. | studies of morphological and ultrastructural alterations in target organs have been useful for evaluating the sublethal effects of biopesticides regarded as safe for non-target organisms in ecotoxicological analyses. one of the most widely used biopesticides is neem oil, and its safety and compatibility with natural enemies have been further clarified through bioassays performed to analyze the effects of indirect exposure by the intake of poisoned prey. thus, this study examined the cellular res ... | 2016 | 26520254 |
| a betabaculovirus-encoded gp64 homolog codes for a functional envelope fusion protein. | the gp64 envelope fusion protein is a hallmark of group i alphabaculoviruses. however, the diatraea saccharalis granulovirus genome sequence revealed the first betabaculovirus species harboring a gp64 homolog (disa118). in this work, we have shown that this homolog encodes a functional envelope fusion protein and could enable the infection and fusogenic abilities of a gp64-null prototype baculovirus. therefore, gp64 may complement or may be in the process of replacing f protein activity in this ... | 2015 | 26537678 |
| a betabaculovirus encoding a gp64 homolog. | a betabaculovirus (disagv) was isolated from diatraea saccharalis (lepidoptera: crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in brazil. | 2016 | 26847652 |
| a two-agent model applied to the biological control of the sugarcane borer (diatraea saccharalis) by the egg parasitoid trichogramma galloi and the larvae parasitoid cotesia flavipes. | the paper is aimed at a methodological development in biological pest control. the considered one pest two-agent system is modelled as a verticum-type system. originally, linear verticum-type systems were introduced by one of the authors for modelling certain industrial systems. these systems are hierarchically composed of linear subsystems such that a part of the state variables of each subsystem affect the dynamics of the next subsystem. recently, verticum-type system models have been applied ... | 2016 | 26911807 |
| selection of prey to improve biological parameters of the predator podisus nigrispinus (dallas, 1851) (hemiptera: pentatomidae) in laboratory conditions. | mass production of predatory stinkbugs in the laboratory is prioritized to release them into the field as part of ipm programs. therefore, the aim of this study was to identify the best prey for rearing the predator podisus nigrispinus (dallas, 1851) (hemiptera: pentatomidae) among five different species of insect (three of lepidoptera, one of coleoptera, and one of diptera). second-instar p. nigrispinus nymphs were conditioned in transparent 1000-ml plastic pots, adults were placed in petri dis ... | 2016 | 26934159 |
| comparative analysis of expression profiling of the trypsin and chymotrypsin genes from lepidoptera species with different levels of sensitivity to soybean peptidase inhibitors. | peptidase inhibitors (pis) are essential proteins involved in plant resistance to herbivorous insects, yet many insect species are able to escape the negative effects of these molecules. we compared the effects of acute and chronic ingestion of soybean peptidase inhibitors (spis) on spodoptera frugiperda and diatraea saccharalis, two lepidoptera species with different sensitivities to spi ingestion. we analyzed the trypsin and chymotrypsin gene expression profiles in both species. acute exposure ... | 2017 | 26944308 |
| proteomic profile of hemolymph and detection of induced antimicrobial peptides in response to microbial challenge in diatraea saccharalis (lepidoptera: crambidae). | insects are organisms extremely well adapted to diverse habitats, primarily due to their innate immune system, which provides them with a range of cellular and humoral responses against microorganisms. lepidoptera hemolymph proteins involved in humoral responses are well known; however, there is a lack of knowledge about the sugarcane borer diatraea saccharalis. in this present work, the hemolymph proteins of this pest insect were studied by applying proteomic methodologies. two-dimensional elec ... | 2016 | 27012208 |
| transgenic sugarcane with a cry1ac gene exhibited better phenotypic traits and enhanced resistance against sugarcane borer. | we developed sugarcane plants with improved resistance to the sugarcane borer, diatraea saccharalis (f). an expression vector pgcry1ac0229, harboring the cry1ac gene and the selectable marker gene, bar, was constructed. this construct was introduced into the sugarcane cultivar fn15 by particle bombardment. transformed plantlets were identified after selection with phosphinothricin (ppt) and basta. plantlets were then screened by pcr based on the presence of cry1ac and 14 cry1ac positive plantlet ... | 2016 | 27093437 |
| reproductive isolation between two populations of diatraea saccharalis (f.) (lepidoptera: crambidae) from different host plant species and regions in argentina. | the sugarcane borer, diatraea saccharalis (f.), has a widespread distribution throughout the western hemisphere and is a pest of many crop plants including sugarcane, corn, sorghum and rice. the use of bacillus thuringiensis (bt) corn has been the primary tool for managing this species in corn fields. sugarcane borer control has been recently threatened by observations of susceptibility and/or resistance to certain varieties of bt corn and the protein used in many newer varieties. this has led t ... | 2016 | 27112423 |
| side effects of neem oil on the midgut endocrine cells of the green lacewing ceraeochrysa claveri (navás) (neuroptera: chrysopidae). | we described the ultrastructure of ceraeochrysa claveri (navás) midgut endocrine cells in larva, pupa, and adult, and evaluated the side effects of ingested neem oil, a botanical insecticide obtained from the seeds of the neem tree (azadirachta indica), on these cells. during the larval period, c. claveri were fed (ad libitum) diatraea saccharalis (f.) eggs treated with neem oil at concentrations of 0.5%, 1%, or 2%. transmission electron microscopy showed that two subtypes of endocrine cells, na ... | 2014 | 27193522 |
| quality of the exotic parasitoid cotesia flavipes (hymenoptera: braconidae) does not show deleterious effects after inbreeding for 10 generations. | although the parasitoid cotesia flavipes (cameron) has proven effective in controlling sugarcane borer diatraea saccharalis (fabricius) for many years, concern has arisen over the quality of individuals produced at large scales. the parasitoid has been reared in laboratories in brazil for more than 40 years, with no new introductions of new populations during that period. since the quality of the parasitoids was not verified at the time of the species' introduction in brazil, we do not know if t ... | 2016 | 27509087 |
| restriction site associated dna (rad) for de novo sequencing and marker discovery in sugarcane borer, diatraea saccharalis fab. (lepidoptera: crambidae). | we present the development of a genomic library using radseq (restriction site associated dna sequencing) protocol for marker discovery that can be applied on evolutionary studies of the sugarcane borer diatraea saccharalis, an important south american insect pest. a radtag protocol combined with illumina paired-end sequencing allowed de novo discovery of 12 811 snps and a high-quality assembly of 122.8m paired-end reads from six individuals, representing 40 gb of sequencing data. approximately ... | 2016 | 27571734 |
| sugarcane serine peptidase inhibitors, serine peptidases, and clp protease system subunits associated with sugarcane borer (diatraea saccharalis) herbivory and wounding. | sugarcane's (saccharum spp.) response to diatraea saccharalis (f.) (lepidoptera: (crambidae) herbivory was investigated using a macroarray spotted with 248 sugarcane expressed sequence tags (ests) encoding serine peptidase inhibitors, serine peptidases. and clp protease system subunits. our results showed that after nine hours of herbivory, 13 sugarcane genes were upregulated and nine were downregulated. among the upregulated genes, nine were similar to serine peptidase inhibitors and four were ... | 2016 | 27598134 |
| possibly similar genetic basis of resistance to bacillus thuringiensis cry1ab protein in 3 resistant colonies of the sugarcane borer collected from louisiana, usa. | the sugarcane borer, diatraea saccharalis (f.), is a major maize borer pest and a target of transgenic maize expressing bacillus thuringiensis (bt) proteins in south america and the mid-southern region of the united states. evolution of resistance in target pest populations is a great threat to the long-term efficacy of bt crops. in this study, we compared the genetic basis of resistance to cry1ab protein in 3 resistant colonies of sugarcane borer established from field populations in louisiana, ... | 2016 | 27860288 |
| microsatellite loci and genetic structure of artificial populations of cotesia flavipes (hymenoptera, braconidae). | cotesia flavipes (cameron) is a parasitoid wasp used in the biological control of the sugarcane borer (diatraea saccharalis) (fabr., 1794). studies on the genetic diversity of c. flavipes are hampered by the lack of highly polymorphic molecular markers. in this report, a set of 11 microsatellite loci were developed from an enriched library of c. flavipes. four microsatellite loci were polymorphic and were screened in 212 c. flavipes individuals (183 females and 29 males) that were randomly sampl ... | 2016 | 27886334 |
| neem oil (azadirachta indica a. juss) affects the ultrastructure of the midgut muscle of ceraeochrysa claveri (navás, 1911) (neuroptera: chrysopidae). | cytomorphological changes, by means of ultrastructural analyses, have been used to determine the effects of the biopesticide neem oil on the muscle fibers of the midgut of the predator ceraeochrysa claveri. insects, throughout the larval period, were fed eggs of diatraea saccharalis treated with neem oil at a concentration of 0.5%, 1% or 2%. in the adult stage, the midgut was collected from female insects at two stages of adulthood (newly emerged and at the start of oviposition) and processed fo ... | 2017 | 27939524 |
| host-plant associated genetic divergence of two diatraea spp. (lepidoptera: crambidae) stemborers on novel crop plants. | diatraea lineolata and diatraea saccharalis (lepidoptera: crambidae) are moths with stemboring larvae that feed and develop on economically important grasses. this study investigated whether these moths have diverged from a native host plant, corn, onto introduced crop plants including sorghum, sugarcane, and rice. diatraea larvae were collected from these four host plants throughout the year in el salvador and were reared on artificial diet until moths or parasitoids emerged. adult moths were s ... | 2016 | 28031813 |
| efficacy of insect growth regulators and diamide insecticides for control of stem borers (lepidoptera: crambidae) in sugarcane. | field experiments assessed control of the stem borers, diatraea saccharalis (f.) and eoreuma loftini (dyar), in sugarcane (saccharum spp.) with selected insect growth regulators and diamide insecticides. tebufenozide, novaluron, chlorantraniliprole, and flubendiamide were evaluated for d. saccharalis control in four field experiments in louisiana between 2009 and 2014. aerial application of the same insecticides was evaluated for control of e. loftini in commercial sugarcane fields in southern t ... | 2017 | 28334153 |