Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| epizootiology, diagnosis, and control of brucella canis. | 1970 | 4987213 | |
| human infection with brucella canis. | 1972 | 5062778 | |
| brucella canis infection in dogs. | 1969 | 5392173 | |
| comments on canine brucellosis due to brucella canis. | 1970 | 5463590 | |
| differentiation of brucella canis from other brucellae by gas chromatography. | gas chromatographic techniques allow for differentiation between a strain of brucella canis and strains of other brucellae. | 1970 | 5498619 |
| epidemic canine brucellosis due to a new species: brucella canis. | 1970 | 5535427 | |
| canine abortion caused by brucella canis. | 1968 | 5688953 | |
| taxonomic position in the genus brucella of the causative agent of canine abortion. | the gram-negative organism causing abortion in dogs was examined in parallel with cultures representative of the brucella species and with bordetella bronchiseptica. the organism fits into the genus brucella and most closely resembles b. suis on the basis of its growth characteristics. it is of rough colonial morphology and is agglutinated by antisera prepared against rough brucella. in mouse toxicity tests, no endotoxic activity could be demonstrated. in contrast to most brucella cultures, it d ... | 1968 | 5689122 |
| eradication of brucella canis infection from a dog colony. | 1968 | 5691834 | |
| properties of brucella canis surface antigens associated with colonial mucoidiness. | rough brucella strains b. abortus (45/20), b. ovis (1182), wild type b. canis (m+) and a less mucoid (m-) variant possessed cell wall antigens that were extracted by both sodium desoxycholate (sdc) and hot phosphate buffered saline (pbs). the acid precipitability of cell wall surface antigens extracted in sdc from all four strains suggests that these antigenic complexes may be responsible for the bacterial aggregation in broth media at acid ph and for the relatively high colonial viscosity on no ... | 1981 | 6172238 |
| effect of combined antibiotic therapy on fertility in brood bitches infected with brucella canis. | bitches with naturally occurring brucella canis infection were treated with combined antibiotic therapy consisting of tetracycline, dihydrostreptomycin, and trimethoprim-sulfadiazine. after treatment, all but 1 bitch became abacteremic, and serologic titers declined for a variable length of time (3 months to 1 years). abortion did not occur while these bitches were abacteremic. although sequential antibiotic therapy for 6 weeks did not eradicate brucella canis from affected bitches, it did not p ... | 1982 | 6178717 |
| brucella canis infection in a 17-month-old child successfully treated with moxalactam. | 1982 | 6215476 | |
| antisperm responses in male dogs with chronic brucella canis infections. | male beagles infected with brucella canis for greater than or equal to 3 months developed serum antibodies that agglutinated normal canine spermatozoa. titers were highest in dogs that had been infected for 4 to 6 months. lower spermagglutinin titers were detected in sera collected 10 months after inoculation. antibodies were also observed in seminal plasma of chronically infected dogs. seminal plasma from infected, but not from clinically normal dogs, caused head-to-head agglutination of normal ... | 1984 | 6231870 |
| multiplication of brucella canis in male reproductive organs and detection of autoantibody to spermatozoa in canine brucellosis. | orchitis, epididymitis and prostatitis have been reported in male dogs infected with brucella canis (b. canis), but the pathogenesis of infertility in male dogs has not been clarified yet. we examined localization of b. canis in the tissue of infected male reproductive organs and production of autoantibody to spermatozoa in male dogs by immunofluorescence and unlabeled antibody peroxidase-antiperoxidase (pap) methods and electron microscopy. b. canis were found in the cytoplasm of macrophages an ... | 1984 | 6386574 |
| immunochemical characterization of rough brucella lipopolysaccharides. | lipopolysaccharides (lps) were extracted from rough strains of brucella abortus and brucella melitensis and from strains of the naturally occurring rough species brucella ovis and brucella canis. brucella rough lipopolysaccharides (r-lps) were readily distinguished from brucella smooth lipopolysaccharides (s-lps) and enterobacterial r-lps, by their chemical, physical, and serological characteristics. b. ovis r-lps was differentiated from b. abortus, b. melitensis, and b. canis r-lps by its react ... | 1984 | 6421737 |
| brucella canis infection in dogs: treatment trials. | 1981 | 6459635 | |
| problems in the serodiagnosis of canine brucellosis: dog responses to cell wall and internal antigens of brucella canis. | three procedures are commonly used for the serodiagnosis of b. canis infection: 1) the rapid slide agglutination test (rsat), 2) the tube agglutination test (tat), and 3) the modified 2-mercaptoethanol tube agglutination test (2me-tat). hemocultures are always essential for diagnosis. the rsat was developed to provide a presumptive diagnosis rapidly. it has been found accurate in identifying non-infected dogs; however false positive reactions are common due to shared determinants between the sur ... | 1984 | 6489621 |
| biological properties and dog response to a variant (m-) strain of brucella canis. | wild type (virulent) b. canis organisms are always rough and growth becomes extremely mucoid (m+) after several days of incubation, especially on media ph 7.2. the mucoid growth of virulent (m+) b. canis and the marked tendency for m+ bacteria, and extracted cell wall antigens, to aggregate at acid ph (ph less than 6.5) are markedly diminished in a less mucoid (m-) variant. the m+ and m- brucella, and their extracted cell wall antigens, also differ in hydrophobic properties; the m+ organisms (an ... | 1984 | 6489639 |
| [a serologic study of human brucella canis infections in the bursa region]. | serum samples of 123 patients of various ages and both sexes from bursa area were tested by mercaptoethanol tube agglutination test on a random basis for br. canis antibodies. two patients, one male and one female, showed high titers (1:400) in mercaptoethanol tube agglutination test. a titer of 1:200 or higher was considered significant and indicative of active infection. br. canis infection in man in turkey is reported for the first time. | 1984 | 6513825 |
| [first isolation of brucella canis in china]. | 1984 | 6532528 | |
| [brucella canis, campylobacter jejuni and df-2 (disgonic fermenter type-2): technical diagnostic profile of emerging canine disease entities of a zoonotic nature]. | 1984 | 6546278 | |
| [brucella canis. serological and bacteriological survey in a feline population]. | 1984 | 6729385 | |
| head-to-head type auto-sperm agglutination with iga antibody to acrosome induced by brucella canis infection. | 1984 | 6737826 | |
| serological evidence for the presence of brucella canis infection in dogs in britain. | a total of 169 dog sera was tested for antibodies to brucella canis and b abortus using rose bengal stained antigens. no antibody to either organism was found in 118 samples. three samples from dogs known to have been in contact with the organism, contained antibody to b abortus. two, both from doberman pinscher bitches with american ancestry but born in britain, contained significant levels of antibody to b canis. one sample which had been taken following caesarian section for a dead pup had an ... | 1980 | 6767306 |
| serological and bacteriological detection of brucella canis infection of stray dogs in moreno, argentina. | a focus of b. canis infection was identified in moreno, argentina, among the stray dog population by serologic methods and confirmed in a second survey which included cultural isolations. a counterimmunoelectrophoresis technique using a specific rough brucella surface antigen was applied to the serodiagnosis of canine brucellosis. this method was found to be as effective as the mercaptoethanol tube agglutination test and the gel diffusion test in detecting b. canis antibodies in natural and expe ... | 1980 | 6775870 |
| binding of bacteria from the genus brucella to human b lymphocytes. | in previous studies, we have shown that various lymphocyte subpopulations bind different strains of bacteria of different genera and species. among these bacteria was a strain of brucella melitensis which bound to all human b lymphocytes. to determine whether the binding of b. melitensis to human b lymphocytes was strain, species, or genus characteristic, we tested the binding of b. melitensis, brucella abortus, brucella ovis, brucella suis, brucella canis and brucella neotomae to human normal a ... | 1981 | 6783546 |
| properties of cell wall antigens of virulent brucella canis and a less mucoid variant of reduced pathogenicity. | properties of cell wall antigenic extracts of 2 brucella canis strains (wild type, m+; and a less mucoid variant, m-) were compared. cell wall antigens of each strain were extracted by 2 different methods and compared with respect to their serologic, chromatographic, and surface properties. in addition, selected enzymes were used to compare antigen sensitivity to degradation. analysis of extracted antigens was done by gel immunodiffusion and immunoelectrophoresis. two cell wall antigens (2-r and ... | 1982 | 6807143 |
| serological responses of dogs to cell wall and internal antigens of brucella canis (b. canis). | 1982 | 6808759 | |
| peripheral lymphocyte function in dogs with brucella canis infection. | peripheral lymphocyte function in dogs with brucella canis infection was evaluated using in vitro lymphocyte stimulation with the mitogens pha, con a and pwm, and killed brucella canis organisms. bitches with naturally occurring brucella canis infection were compared to negative controls. there was no difference in the response to con a and pwm between these two groups. lymphocytes from infected dogs were less responsive (p less than .05) to pha than were lymphocytes from controls. there was a s ... | 1983 | 6868338 |
| brucella canis infection in a kennel. | 1980 | 6900450 | |
| improved rapid slide agglutination test for presumptive diagnosis of canine brucellosis. | a modified rapid slide agglutination test for the presumptive identification of brucella canis infection in dogs has been developed. the method required mixing 0.1 ml of canine serum with 0.1 ml of 0.2 m 2-mercaptoethanol solution. equal volumes (0.05 ml) of the treated serum and the b. canis plate antigen were mixed. agglutination results were read within 2 min. clinical studies showed 100% agreement between this method and the conventional 2-mercaptoethanol tube agglutination test. excellent c ... | 1982 | 7068825 |
| a microagglutination test for human brucella canis antibodies. | a microagglutination test for the detection of brucella canis antibodies in humans is described. the use of safranin-dyed b. canis organisms as antigen gave easily interpretable settling patterns and reproducible endpoints. the finding of 18 of 1,147 sera with titers greater than or equal to 1:160 resulted in a presumed false-positive rate of 1.6%. repeat testing of these positive sera in diluent to which 0.6 m nacl had been added showed a decrease in titer to less than or equal to 1:40 in eight ... | 1982 | 7091053 |
| effect of a two-stage antibiotic treatment regimen on dogs naturally infected with brucella canis. | 1982 | 7096187 | |
| human brucellosis caused by brucella canis: clinical features and immune response. | human infection by brucella canis is not often recognized due to a lack of serious consideration of the disease as a diagnostic possibility. another factor is the limited availability of specific serologic tests needed in the absence of cross-reactivity between antibodies to b. canis and other brucella pathogenic to humans. in addition, the organism may not be isolated from blood culture, particularly after antibiotic therapy. we report clinical and laboratory features of four patients with sero ... | 1982 | 7137736 |
| pyogranulomatous dermatitis associated with brucella canis infection in a dog. | 1982 | 7174496 | |
| [brucellosis in the cat. experimental infection with brucella canis]. | 1982 | 7187858 | |
| [serological determination of brucella canis infections using the slide agglutination test]. | 1980 | 7213330 | |
| [detection of brucella canis antibodies in serum samples of military service dogs]. | 1981 | 7213335 | |
| [research on anti-brucella canis agglutinins in human sera in the city of são paulo, brazil]. | 1980 | 7233061 | |
| brucella canis in a uterine stump abscess in a bitch. | 1981 | 7240018 | |
| isolation of brucella canis. | these strains of brucella canis were isolated from seropositive dogs: the strains es 11/78, from a kennel dog, and the strains 215/79 and pr/79, from stray dogs. the isolates were identified through the morphological, staining and biochemical characteristics (of the agent) as well as serologically, by the use of the isolated strains as antigen against the hyperimmune serum. | 1980 | 7251257 |
| long-term observation of canine brucellosis: excretion of brucella canis into urine of infected male dogs. | six male dogs orally inoculated with 3.2 x 10(8) brucella canis and 2 male dogs naturally infected with the organisms were examined weekly for the presence of the organisms in urine and blood as well as for agglutinin titers in serum. excretion of the organisms into urine started on 1 to 3 weeks after onset of bacteremia, i.e. 4 to 8 weeks after inoculation, and lasted for about 1 to 1.5 years. non-bacteremic intervals, intermittent or lasting for about 1 to 6 months, followed the initial period ... | 1981 | 7262203 |
| cellular fatty acids of brucella canis and brucella suis. | the cellular fatty acid composition of brucella canis and brucella suis was determined by gas-liquid chromatography. the presence of relatively large amounts of a 19-carbon cyclopropane fatty acid in b. suis was a major distinguishing feature between these organisms. the gas-liquid chromatography test for cellular fatty acids provides an additional criterion for the distinction of antigenically rough strains of b. suis which cannot be differentiated from b. canis by conventional procedures. | 1981 | 7263850 |
| spermagglutination and spermagglutinating activity of serum and tissue extracts from reproductive organs in male dogs experimentally infected with brucella canis. | 1981 | 7339137 | |
| brucella canis infection. | 1980 | 7361393 | |
| a serological survey of dogs for brucella canis in southwestern ontario. | sera from 2 000 dogs from southwestern ontario were tested for antibodies to brucella canis by a rapid slide agglutination test. the 100 positively reacting sera were tested by tube agglutination and immunoprecipitation (gel diffusion) tests. thirty-one of these sera gave suspicious titres and one a positive titre in the tube agglutination test. six of the rapid slide test positive sera gave positive reactions in immunoprecipitation tests. one dog was identified which was found to have a history ... | 1980 | 7427846 |
| [serodiagnosis of brucella canis infections by comparative slow agglutination at 37 degrees c and 50 degrees c]. | 1980 | 7445834 | |
| diskospondylitis in dogs. | during a 3-year period, diskospondylitis was diagnosed in 32 dogs. the condition affected primarily large dogs, with males outnumbering females by approximately 2:1. diagnosis was made principally by evaluation of radiographs, wherein various degrees of vertebral lysis, sclerosis, and spondylosis were seen. evaluation of titers for brucella canis and bacterial cultures of blood, urine, and bone were used to identify the causative organism(s). six of 24 dogs were seropositive for b canis, 21 of 2 ... | 1980 | 7451303 |
| in vitro efficacy of several antimicrobial combinations against brucella canis and brucella melitensis strains isolated from dogs. | in vitro efficacy of tetracyclines, aminoglycosides, fluoroquinolones, rifampin and macrolides and their combinations against four brucella canis and two b. melitensis strains isolated from dogs was evaluated by means of inhibition tests: minimal inhibitory concentration (mic) and fractional inhibitory concentrations (fic) and by bactericidal tests: minimal bactericidal concentrations (mbc) and kill-time experiments. in inhibition studies, tetracyclines showed the lowest mics of all compounds te ... | 1995 | 7653024 |
| differentiated u937 cells exhibit increased bactericidal activity upon lps activation and discriminate between virulent and avirulent listeria and brucella species. | in the study of interactions between facultative intracellular pathogens and macrophages, monocytic cell lines have the advantages of showing defined states of activation and lacking genetic variation among donors, thus yielding reproducible results. nonpathogenic escherichia coli k12 were killed at similar rates in the u937 cell line differentiated into macrophage-like cells by phorbol myristate acetate (pma) or by the combination of retinoic acid (ra) and vitamin d3 (vd). complete elimination ... | 1994 | 8071593 |
| identification of an immunoreactive brucella abortus htra stress response protein homolog. | an 11-kb fragment of brucella abortus genomic dna cloned into the bamhi site of puc9 expressed a 60-kda protein in escherichia coli dh5-alpha. antibodies reactive with this 60-kda protein were detected by western blot (immunoblot) analysis in sera from mice, cattle, and goats experimentally infected with b. abortus, in sera from mice experimentally infected with brucella melitensis, and in serum from a dog experimentally infected with brucella canis. similar results were seen with sera obtained ... | 1994 | 8112833 |
| use of indirect enzyme-linked immunosorbent assay with hot saline solution extracts of a variant (m-) strain of brucella canis for diagnosis of brucellosis in dogs. | an elisa, using hot saline solution extracts (hss) of a less-mucoid variant (m-) strain of brucella canis as antigen, was developed for detection of antibodies against b canis in dogs. the test was applied to 177 field serum samples previously tested by use of the 2-mercaptoethanol rapid slide agglutination test, 2-mercaptoethanol-tube agglutination test, and agar gel immunodiffusion containing hss and cytoplasmic antigens of b. canis. results indicated that this elisa seems to be highly specifi ... | 1993 | 8368596 |
| nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of brucella ovis: evidence for antigenic shift, compared with other brucella species, due to a deletion in the gene. | the nucleotide sequences encoding the major 25-kda outer membrane protein (omp) (omp25 genes) of brucella ovis 63/290, brucella melitensis 16m, brucella suis 1330, brucella canis rm6/66, and brucella neotomae 5k33 (all reference strains) were determined and compared with that of brucella abortus 544 (p. de wergifosse, p. lintermans, j. n. limet, and a. cloeckaert, j. bacteriol. 177:1911-1914, 1995). the major difference found was between the omp25 gene of b. ovis and those of the other brucella ... | 1996 | 8675306 |
| canine brucellosis: a diagnostician's dilemma. | salient features of brucellosis in dogs caused by brucella canis are briefly reviewed, with emphasis on problems and pitfalls in the serodiagnosis of the disease. procedures available to veterinarians for the diagnosis of canine brucellosis are discussed from the point of view of test sensitivity and specificity. the serodiagnosis of the disease poses a particular dilemma because several tests in current use by diagnostic laboratories have not been evaluated critically and may provide false-posi ... | 1996 | 8942212 |
| serological survey for diseases in free-ranging coyotes (canis latrans) in yellowstone national park, wyoming. | from october 1989 to june 1993, we captured and sampled 110 coyotes (canis latrans) for various diseases in yellowstone national park, wyoming (usa). prevalence of antibodies against canine parvovirus (cpv) was 100% for adults (> 24 months old), 100% for yearlings (12 to 24 months old), and 100% for old pups (4 to 12 months old); 0% of the young pups (< 3 months old) had antibodies against cpv. presence of antibodies against canine distemper virus (cdv) was associated with the age of the coyote, ... | 1997 | 9027690 |
| [comparative study of the antigenic composition of brucella canis strains by immunoblotting]. | brucella cultures isolated from sick dogs have not been properly studied in russia up to the present time. in 1994 a culture has been isolated from aborted fetus of a dog. investigations by the traditional methods referred it to brucella genus, species canis (strain k-01). reference strain b. canis rm6/66 and b. canis k-01 were identical by the profiles of protein antigens in immunoblotting with a set of antibrucellosis sera. b. canis, b. suis, b. abortus, and b. melitensis. however, immunoblott ... | 1997 | 9297104 |
| dna polymorphism at the omp-31 locus of brucella spp.: evidence for a large deletion in brucella abortus, and other species-specific markers. | the omp-31 gene, encoding a major outer-membrane protein in brucella melitensis, was pcr-amplified from brucella strains representing all species and known biovars by using primers selected according to the b. melitensis 16m omp-31 published sequence. amplification of omp-31 was achieved from dna of all brucella species with the exception of brucella abortus, the only brucella species where expression of omp-31 was not detected by reactivity with an mab specific for an epitope located in omp-31. ... | 1997 | 9308175 |
| transposon-derived brucella abortus rough mutants are attenuated and exhibit reduced intracellular survival. | the o antigen of brucella abortus has been described as a major virulence determinant based on the attenuated survival of fortuitously isolated rough variants. however, the lack of genetic definition of these mutants and the virulence of naturally occurring rough species, brucella ovis and brucella canis, has confused interpretation. to better characterize the role of o antigen in virulence and survival, transposon mutagenesis was used to generate b. abortus rough mutants defective in o-antigen ... | 1998 | 9488389 |
| identification of brucella by ribosomal-spacer-region pcr and differentiation of brucella canis from other brucella spp. pathogenic for humans by carbohydrate profiles. | molecular and chemical characteristics often provide complementary information in the differentiation of closely related organisms. the genus brucella consists of a highly conserved group of organisms. identification of the four species pathogenic in humans (brucella melitensis, brucella abortus, brucella suis, and brucella canis) is problematic for many clinical laboratories that depend primarily on serology and phenotypic characteristics to differentiate species. pcr amplification of the 16s-2 ... | 1998 | 9774568 |
| mycotic aneurysms of the tibioperoneal arteries. | this case report describes a pediatric patient with mycotic aneurysms of both posterior tibial arteries and the right peroneal artery associated with an episode of endocarditis. to our knowledge, this case represents the first reported occurrence of multiple mycotic aneurysms of the tibioperoneal arteries. it is also unique in that the pathogen was brucella canis instead of the usual gram-positive pathogens associated with intravenous drug injection. vascular reconstruction can be accomplished; ... | 1999 | 10072460 |
| brucella canis endocarditis: case report. | 1999 | 10585833 | |
| diskospondylitis and other vertebral infections. | this article discusses infections of the spine, including diskospondylitis, vertebral osteomyelitis, and vertebral physitis. spinal pain is the most common clinical sign. plain radiography is usually diagnostic; computed tomography and magnetic resonance imaging are useful when the diagnosis is unclear. serology for brucella canis and cultures of blood and urine are important in identifying the cause. appropriate antibiotic therapy is successful in the majority of cases of bacterial spinal infec ... | 2000 | 10680214 |
| brucellosis: unusual presentations in two adolescent boys. | two boys presented with variable signs and symptoms of infectious disease that challenged diagnosis. one of the two patients had aortic valve vegetations and lower extremity aneurysms, and the other had calvarial osteomyelitis, epidural abscess, pleural effusions, and pulmonary nodules. only after a battery of bacterial and fungal agglutination tests was the unsuspected diagnosis made in each of brucellosis from brucella canis. | 2000 | 10836605 |
| limited genetic diversity of brucella spp. | multilocus enzyme electrophoresis (mlee) of 99 brucella isolates, including the type strains from all recognized species, revealed a very limited genetic diversity and supports the proposal of a monospecific genus. in mlee-derived dendrograms, brucella abortus and a marine brucella sp. grouped into a single electrophoretic type related to brucella neotomae and brucella ovis. brucella suis and brucella canis formed another cluster linked to brucella melitensis and related to rhizobium tropici. th ... | 2001 | 11136777 |
| the brucella suis homologue of the agrobacterium tumefaciens chromosomal virulence operon chve is essential for sugar utilization but not for survival in macrophages. | brucella strains possess an operon encoding type iv secretion machinery very similar to that coded by the agrobacterium tumefaciens virb operon. here we describe cloning of the brucella suis homologue of the chve-ggua-ggub operon of a. tumefaciens and characterize the sugar binding protein chve (78% identity), which in a. tumefaciens is involved in virulence gene expression. b. suis chve is upstream of the putative sugar transporter-encoding genes ggua and ggub, also present in a. tumefaciens, b ... | 2001 | 11514518 |
| sensitivity and specificity of an indirect enzyme-linked immunoassay for the diagnosis of brucella canis infection in dogs. | the diagnosis of b. canis infection in dogs is based on bacteriological examination and serological methods including agglutination and gel diffusion tests. bacteriological studies are the only methods that have been considered specific but, as intermittent periods of abacteraemia may occur, a negative blood culture cannot be used as a criterion for excluding canine brucellosis. close contact between people and infected dogs increases the risk of transmission; however, its impact on public healt ... | 2002 | 12171296 |
| canine brucellosis in a dog imported into the uk. | 2002 | 12219905 | |
| comparative performance of tests using cytosolic or outer membrane antigens of brucella for the serodiagnosis of canine brucellosis. | although some elisa tests using cytoplasmic or outer membrane antigens of brucella have been developed to improve the diagnosis of canine brucellosis, the performance of these assays has not been compared. in the present study three elisa tests using lipopolysaccharide (lps)-free cytoplasmic proteins (cps) of brucella abortus, the lumazine synthase (ls) of brucella spp. or a hot-saline (hs) extract of brucella canis containing outer membrane antigens were used to test sera from dogs with suspect ... | 2002 | 12220811 |
| serological diagnosis of brucellosis caused by brucella canis. | blood serum samples from 2,328 dogs were tested to detect antibodies against brucella canis with the agar gel immunodiffusion (agid) and 2-mercaptoethanol slide agglutination test (me-sat) using brucella ovis as the antigen. all blood serum samples were also evaluated for antibodies against brucella abortus and brucella melitensis using the rose bengal test. twentyfive (1.07%) of the sera evaluated were considered positive with agid test. only 4 (16%) of these blood serum samples were positive w ... | 2003 | 12578313 |
| production of the type iv secretion system differs among brucella species as revealed with virb5- and virb8-specific antisera. | expression of the virb operon, encoding the type iv secretion system required for brucella suis virulence, occurred in the acidic phagocytic vacuoles of macrophages and could be induced in minimal medium at acidic ph values. to analyze the production of virb proteins, polyclonal antisera against b. suis virb5 and virb8 were generated. western blot analysis revealed that virb5 and virb8 were detected after 3 h in acidic minimal medium and that the amounts increased after prolonged incubation. unl ... | 2003 | 12595417 |
| the immunologic response of dogs to bartonella vinsonii subspecies berkhoffii antigens: as assessed by western immunoblot analysis. | bartonella vinsonii subspecies berkhoffii is a recently recognized zoonotic pathogen that causes endocarditis, granulomatous rhinitis, and granulomatous lymphadenitis in dogs. isolation of b. vinsonii (berkhoffii) from blood or tissue samples is frequently unsuccessful; therefore, diagnosis is primarily dependent on serologic or molecular testing modalities. because previous canine serologic studies have used an indirect immunofluorescence assay (ifa), without western immunoblot (wi) confirmatio ... | 2003 | 12918816 |
| identification of species of brucella using fourier transform infrared spectroscopy. | fourier transform infrared spectroscopy (ftir) is a technique that has been used over the years in chemical analysis for the identification of substances and is one that may be applied to the characterisation of microorganisms. the marked tendency of brucella towards variation in the smooth rough phase, together with the laboriousness and risk involved in the methods used in their identification, make their classification difficult. we studied the type strains of the different species and biovar ... | 2003 | 14500003 |
| [canine brucellosis--a case report]. | brucella (b.) canis was isolated from ejaculate of a 4-year old korthals-griffon male dog after occurrence of epididymitis and orchitis. despite several trials of therapy with different antibiotics relapes occurred, with b. canis being isolated from ejaculate, blood and urine samples, respectively. bacteriological examinations were added by serological testing over a period of about 1.5 years. during the study sat serum titre steadily dropped from 1:200 to 1:50. by cft, b. canis antibodies were ... | 2003 | 14526466 |
| human infection with m- strain of brucella canis. | the less mucoid strain of brucella canis or m- strain is used for the serologic diagnosis of canine brucellosis. while this strain is avirulent in dogs, we report the case of clinical brucellosis that developed in a laboratory worker a few days after handling live m- cells for antigen production. | 2004 | 15078613 |
| rough lipopolysaccharide of brucella abortus rb51 as a common antigen for serological detection of b. ovis, b. canis, and b. abortus rb51 exposure using indirect enzyme immunoassay and fluorescence polarization assay. | rough lipopolysaccharide (rlps) antigens were prepared from cultures of brucella abortus rb51, b. ovis, and b. canis. the preparations were standardized by weight and tested with sera from cattle immunized with b. abortus rb51, sheep infected with b. ovis, and dogs infected with b. canis. populations of unexposed animals of each species were also tested. the tests used were the indirect enzyme immunoassay (ielisa) using rlps and the fluorescence polarization assay (fpa) using rlps core fractions ... | 2004 | 15162920 |
| infection and blood transfusion: a guide to donor screening. | in recent years, blood-component therapy has become more accessible in veterinary practice. as with human medicine, care must be taken to minimize the risk of disease transmission from donor to recipient. determining the appropriate diseases to screen for is complicated by regional variations in disease incidence, the existence of chronic carrier states for some diseases, the difficulty in screening-test selection, and testing cost. the feline diseases considered include retroviral infections, f ... | 2004 | 15179926 |
| clinical resolution of brucella canis-induced ocular inflammation in a dog. | a 2-year-old spayed female mixed-breed dog was referred with an 8-month history of recurrent bilateral anterior uveitis and hyphema secondary to infection with brucella canis. treatment with doxycycline po and atropine and prednisone acetate topically prior to referral had failed to resolve the ocular inflammation. successful control of ocular inflammation was achieved after initiation of treatment with gentamicin sc and ciprofloxacin and doxycycline po. the finite indirect fluorescent antibody ... | 2004 | 15198266 |
| canine brucellosis. | this review discusses the prevalence, etiology, pathogenesis, clinical findings, diagnostic methods, therapy, management and public health considerations of brucella canis infection in dogs. canine brucellosis is a contagious infection produced by a gram-negative coccobacilus called brucella canis. the main sources of infection are vaginal fluids of infected females and urine in males. routes of entry are venereal, oronasal, conjunctivae mucosa and placenta. the most significant symptoms are lat ... | 2004 | 15271453 |
| occurrence and potential diagnostic applications of serological cross-reactivities between brucella and other alpha-proteobacteria. | agrobacterium, sinorhizobium, and ochrobactrum are genera closely related to brucella but, in contrast to the latter, are not pathogenic for humans and animals. we studied by an indirect enzyme-linked immunosorbent assay (elisa) the reactivities of brucellosis sera against cytosolic (cyt) and membrane (ma) antigens from these nonpathogenic bacteria, and we evaluated the potential usefulness of these cross-reactions for the diagnosis of brucellosis in humans, sheep, cows, and dogs. canine infecti ... | 2004 | 15358645 |
| [bacteremia by brucella canis. isolation with the bact-alert system]. | brucella canis and other species of the genus brucella can cause human disease. however, this species infrequently cause human disease, including in countries where dogs population is highly infected. a 15 years old male was admitted to the hospital with 15 days history of fever without visible focus. physical examination revealed pain at liver palpation and axillar, cervical and inguinal lymphoadenomegalies. abdominal ultrasonography showed spleenomegally, the chest rx and the trans thoracic ec ... | 2004 | 15470867 |
| use of brucella canis antigen for detection of ovine serum antibodies against brucella ovis. | brucella ovis causes a genital disease of sheep manifested by epididymitis in rams and placentitis in ewes producing reduced fertility in the flock. clinical diagnosis is not sensitive enough and bacteriological testing is not feasible for detection of the disease in large numbers of animals. indirect methods of serological testing are preferred for routine diagnosis, of which agar gel immunodiffusion (agid), complement fixation (cf) and elisa tests are recommended as the most efficient. since b ... | 2004 | 15708814 |
| diagnosis of human brucellosis caused by brucella canis. | the transmission of brucella canis to man commonly occurs through contact with infected dogs or their secretions, or through direct laboratory exposure. the disease is underdiagnosed due to a general lack of serological testing facilities and misconceptions concerning its prevalence. this report shows the potential use of an indirect elisa (ielisa) for the diagnosis of human brucellosis caused by b. canis in a population of patients negative by smooth-brucella antigen tests but positive by rapid ... | 2005 | 15824423 |
| unusual clinical presentation of brucellosis caused by brucella canis. | brucella canis is considered a rare cause of human brucellosis. the clinical importance of this infection may have been underestimated so far because of difficulties with presumptive diagnosis. the case described here presented symptoms compatible with brucellosis but the routine tests using brucella abortus antigen were negative. the infection would have remained undiagnosed if culture had not been positive. this case illustrates the potential for a favourable outcome in brucella canis diagnosi ... | 2005 | 15824432 |
| hypoluteoidism in a bitch. | hypoluteoidism is characterized by insufficient secretion of progesterone by the corpora lutea during pregnancy. the resulting failure to maintain progesterone concentration above a critical level presumably could lead to fetal resorbtion as well as frank abortion. this report concerns a 2.5-year-old bernese mountain dog with a history of two previous pregnancies ending in abortion around day 50 of pregnancy. the bitch was initially presented 2 days after mating. physical and gynecological exami ... | 2005 | 15935854 |
| brucella canis in south africa. | 2005 | 16299376 | |
| serologic survey of select infectious diseases in coyotes and raccoons in nebraska. | to obtain data about select zoonotic and other infectious diseases in free-ranging predators in five ecoregions in nebraska, sera were collected from 67 coyotes (canis latrans) and 63 raccoons (procyon lotor) from november 2002 through january 2003. for coyotes, antibodies were detected against canine distemper virus (cdv, 61%), francisella tularensis (32%), rickettsia rickettsi (13%), and flaviviruses (48%). none of the coyote sera had antibodies to borrelia burgdorferi, brucella canis, or six ... | 2005 | 16456169 |
| use of enrofloxacin in the treatment of canine brucellosis in a dog kennel (clinical trial). | to date, no totally effective antibiotic for the eradication of canine brucellosis has been found. the purpose of this study was to evaluate the efficacy of enrofloxacin in a kennel infected with brucella canis. twelve dogs, 2 males and 10 females (including 1 in estrus, 3 pregnant, and 6 in anestrus) infected with b. canis were given 5 mg/kg of enrofloxacin orally every 12 h for 30 days. females received additional courses of enrofloxacin during the estral and luteal phases of the subsequent cy ... | 2006 | 16476476 |
| detection of antibodies to brucella ovis in sheep milk using b. ovis and b. canis antigen. | the diagnostic techniques most widely used for detecting brucellosis caused by brucella ovis are serological tests such as complement fixation (cft), agar gel immunodiffusion (agid), and elisas. however, to our knowledge, milk tests, with the advantage that samples may be taken in a non invasive manner, have not been investigated as diagnostic tools. we studied 144 samples of milk and sera from lactating ewes, comparing bacteriological studies, serological and milk tests using brucella canis and ... | 2006 | 16678362 |
| canine brucellosis: outbreaks and compliance. | canine infertility has many causes that must be considered during evaluation of abnormal reproductive function. an important infectious agent is brucella canis. classically deemed a major reason of abortion, this organism also produces infertility in stud dogs and poses a potential health hazard to dogs and humans. the state of georgia has, out of necessity, instigated regulations to manage outbreaks and seek compliance by educating the pet owner population about this disease. a review of its et ... | 2006 | 16716382 |
| detection of brucella canis and leptospira interrogans in canine semen by multiplex nested pcr. | brucella canis and leptospira interrogans are pathogenic bacteria that cause brucellosis and leptospirosis in dogs around the world. both diseases can be diagnosed serologically, but the direct detection of these organisms in canine semen is needed when it is used for artificial reproduction. we have been attempting the artificial reproduction of guide dogs for greater breeding efficiency and for this purpose have developed a multiplex nested pcr technique for the detection of b. canis and l. in ... | 2006 | 16820720 |
| diagnosis of canine brucellosis: comparison between serological and microbiological tests and a pcr based on primers to 16s-23s rdna interspacer. | a pair of primers directed to 16s-23s rdna interspacer (its) was designed directed to brucella genetic sequences in order to develop a polymerase chain reaction (pcr) putatively capable of amplifying dna from any brucella species. nucleic acid extracts from whole-blood from naive dogs were spiked with decreasing amounts of brucella canis rm6/66 dna and the resulting solutions were tested by pcr. in addition, the ability of pcr to amplify brucella spp. genetic sequences from naturally infected do ... | 2007 | 17308888 |
| investigation of the spread of brucella canis via the u.s. interstate dog trade. | the aim of this study was to illustrate and help address a growing need for regulatory or molecular tools to track and control the spread of canine brucellosis. our study objectives were to first characterize brucella canis outbreaks in wisconsin kennels in the context of the dog trade in the usa, and then to identify a molecular technique that may be useful for strain differentiation of b. canis isolates. | 2007 | 17331783 |
| a polymerase chain reaction for the detection of brucella canis in semen of naturally infected dogs. | the objective was to evaluate a pcr assay for the detection of brucella canis in canine semen, comparing its performance with that of bacterial isolation, serological tests and pcr assay of blood. fifty-two male dogs were examined clinically to detect reproductive abnormalities and their serum was tested by the rapid slide agglutination test, with and without 2-mercaptoethanol (2me-rsat and rsat, respectively). in addition, microbiological culture and pcr assays were performed on blood and semen ... | 2007 | 17343907 |
| brucella canis isolates from canadian dogs. | eleven brucella canis isolates from canadian dogs were characterized by dye and antibiotic sensitivity, phage susceptibility, urease and h(2)s production, co(2) requirement, and reaction with monospecific a,m, and r anti-brucella antiserum. the isolates could be separated into two distinct groups. one group had a sensitivity pattern similar to that seen with the american type strain rm666, while the other group had a pattern identical to that of a mexican strain, mex 51. epidemiological studies ... | 1988 | 17422968 |
| diagnosis of canine brucellosis by elisa using an antigen obtained from wild brucella canis. | an indirect elisa test was developed for the diagnosis of brucella canis infection in dogs. a bacterial whole cell extract was used as a solid phase antigen, using b. canis isolated from an infected animal. sera from culture-positive and healthy negative animals were used as internal reference controls. the cut-off point was determined by a mathematical formula for a statistically valid value, which defined the upper prediction limit, based on the upper tail of the t-distribution of 21 negative ... | 2007 | 17442351 |
| serosurvey of infectious disease agents of carnivores in captive red pandas (ailurus fulgens) in china. | the future of the endangered red panda (ailurusfulgens) depends in part on the development of protective measures against infectious diseases. the present study is a first step toward improved understanding of infectious diseases in the species' home regions. serum samples obtained from 73 red pandas in 10 captive facilities in southwest, east, and northeast china from october to december 2004 were tested for antibodies against nine common infectious pathogens of carnivores. antibody titers agai ... | 2007 | 17469274 |
| a rapid agglutination assay for canine brucellosis using antigen coated beads. | brucella canis is the causative agent of canine brucellosis and facultative intracellular pathogen. the diagnosis of canine brucellosis is based on bacteriological examination and serological methods including agglutination and gel diffusion tests. in this study, crude antigens were extracted from b. canis using hot saline, coated on to latex beads and their usefulness in the serological diagnosis of canine brucellosis was examined. mixing the antigen coated latex beads with the sera of dogs inf ... | 2007 | 17551219 |
| theriogenology question of the month. the infected bitch should be euthanized. the other dogs in the kennel should be quarantined. | 2007 | 17867968 | |
| a polymerase chain reaction for detection of brucella canis in vaginal swabs of naturally infected bitches. | a pcr assay for the detection of brucella canis in canine vaginal swab samples was evaluated, comparing its performance with that of bacterial isolation, serological tests, and a blood pcr assay. one hundred and forty-four female dogs were clinically examined to detect reproductive problems and they were tested by the rapid slide agglutination test, with and without 2-mercaptoethanol (2me-rsat and rsat, respectively). in addition, microbiological culture and pcr were performed on blood and vagin ... | 2007 | 17920673 |
| evaluation of immunochromatographic assay for serodiagnosis of brucella canis. | canine brucellosis is a contagious disease with venereal and oral modes of transmission that produces late abortion in females, epididymides and prostates in males. diagnosis is difficult because of unstable serum antibody titers that vary from individual to individual as well as between different methods used for their detection. the objective of this work was to evaluate the clinical utility of the immunochromatographic assay (ica) for serodiagnosis of dogs suspected of having brucellosis, and ... | 2007 | 18057823 |