Publications

brenneria salicis, the bacterium causing watermark disease in willow, resides as an endophyte in wood.	brenneria salicis has been studied in willow wood only in relation to watermark disease. in this pathogenic condition, the bacterium occurs at high concentrations. pathogenicity of b. salicis is still uncontrollable and the disease unpredictable because the plant-bacteria interaction is not understood. thanks to molecular techniques b. salicis can be detected at low concentrations, which are found in most non-pathogenic interactions. brenneria salicis was identified and traced with a new specifi ...	2009	19220404
six cases of sepsis caused by pantoea agglomerans in a teaching hospital.	pantoea agglomerans is an environmental organism which may seldom cause opportunistic infections. here we report on a 6 case outbreak in a teaching hospital. within three months . agglomerans was isolated from blood cultures of 5 patients from oncology and 1 patient from icu departments. p. agglomerans was in pure culture in 5 cases, while in the last one rahnella aquatilis and candida famata were also isolated. therefore, p. agglomerans is able to produce nosocomial infections in patients with ...	2009	19382678
isolation and structure elucidation of two different polysaccharides from the lipopolysaccharide of rahnella aquatilis 33071t.	two different polysaccharides were obtained by mild acid degradation of the lipopolysaccharide of rahnella aquatilis 33071(t). these were studied by sugar and methylation analyses along with 1d and 2d (1)h and (13)c nmr spectroscopy. the following structures were established for the polysaccharides: -->4)-alpha-d-rhap-(1-->3)-alpha-d-rhap-(1-->3)beta-d-galp-(1-->3)-alpha-d-manp-(1-->2)-alpha-d-manp-(1--> beta-d-glcp-(1-->3)-alpha-d-galp-(1-->4)-alpha-d-glcpa-(1-->2). the former structure is new, ...	2009	19442965
enterobacteriaceae facilitate the anaerobic degradation of glucose by a forest soil.	anoxic micro zones that occur in soil aggregates of oxic soils may be temporarily extended after rainfall and thus facilitate the anaerobic degradation of organic compounds in soils. the microbial degradation of glucose by anoxic slurries of a forest soil yielded acetate, co2, h2, succinate, and ethanol, products indicative of mixed acid fermentation. prokaryotes involved in this process were identified by time-resolved 16s rrna gene-targeted stable isotope probing with [13c-u]-glucose. all labe ...	2009	19453494
biogeochemical changes induced in uranium mining waste pile samples by uranyl nitrate treatments under anaerobic conditions.	response of the subsurface soil bacterial community of a uranium mining waste pile to treatments with uranyl nitrate over different periods of time was studied under anaerobic conditions. the fate of the added u(vi) without supplementation with electron donors was investigated as well. by using 16s rrna gene retrieval, we demonstrated that incubation with uranyl nitrate for 4 weeks resulted in a strong reduction in and even disappearance of some of the most predominant bacterial groups of the or ...	2009	19476503
role of metal resistant plant growth promoting bacteria in ameliorating fly ash to the growth of brassica juncea.	in this study, we have shown that the plant growth promoting bacterial strain nbri k24 and strain nbri k3 from fly ash (fa) contaminated soil reduce the toxicity of ni and cr in brassica juncea (indian mustard) and promote plant growth under pot culture experiments. isolated strains nbri k24 and nbri k3 were characterized based on the 16s rdna sequencing and identified as enterobacter aerogenes and rahnella aquatilis respectively. both the strains were siderophore producing and found capable of ...	2009	19487076
bacterial community and nitrogen fixation in the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae).	the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae), colonizes all pines species within its native range throughout north and central america. recently, this species was accidentally introduced to china, where it has caused severe damage in pine forests. it belongs to a group of beetles that spend most of their lives between the tree bark and sapwood, where it feeds on phloem: a poor substrate with very low nutritional value of nitrogen and toxic proper ...	2009	19543937
mutations that disrupt either the pqq or the gdh gene of rahnella aquatilis abolish the production of an antibacterial substance and result in reduced biological control of grapevine crown gall.	rahnella aquatilis hx2, a biocontrol agent for grapevine crown gall caused by agrobacterium vitis, produces an antibacterial substance that inhibits the growth of a. vitis in vitro. in this study, we show that mh15 and mh16, two tn5-induced mutants of hx2, have lost their abilities to inhibit a. vitis and have reduced biocontrol activities; they grow in logarithmic phase at a rate similar to that of the wild type and have single tn5 insertions. they are also impaired in producing pyrroloquinolin ...	2009	19734331
microbial diversity changes in soybean sprouts treated with enterocin as-48.	seed sprouts may act as vehicles for foodborne pathogenic bacteria. in the present study, the effect of washing treatment with the enterococcal bacteriocin enterocin as-48 on the microbiota of two batches of soybean sprouts was studied by culture-dependent and independent methods throughout storage at 10 degrees c. viable cell counts of bacteriocin-treated samples revealed some modifications only for lactic acid bacteria and enterococci during storage. in the control samples from batch 1, the cu ...	2009	19835782
organic and conventional fruits and vegetables contain equivalent counts of gram-negative bacteria expressing resistance to antibacterial agents.	resistance to antibiotics is a major public health problem which might culminate in outbreaks caused by pathogenic bacteria untreatable by known antibiotics. most of the genes conferring resistance are acquired horizontally from already resistant commensal or environmental bacteria. food contamination by resistant bacteria might be a significant source of resistance genes for human bacteria but has never been precisely assessed, nor is it known whether organic products differ in this respect fro ...	2010	19919536
16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens.	a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ...	2009	19937215
frequency and diversity of small cryptic plasmids in the genus rahnella.	rahnella is a widely distributed genus belonging to the enterobacteriaceae and frequently present on vegetables. although rahnella has interesting agro-economical and industrial properties and several strains possess antibiotic resistances and toxin genes which might spread within microbial communities, little is known about plasmids of this genus. thus, we isolated a number of rahnella strains and investigated their complements of small plasmids.	2010	20170524
seasonal and regional diversity of maple sap microbiota revealed using community pcr fingerprinting and 16s rrna gene clone libraries.	an arbitrary primed community pcr fingerprinting technique based on capillary electrophoresis was developed to study maple sap microbial community characteristics among 19 production sites in québec over the tapping season. presumptive fragment identification was made with corresponding fingerprint profiles of bacterial isolate cultures. maple sap microbial communities were subsequently compared using a representative subset of 13 16s rrna gene clone libraries followed by gene sequence analysis. ...	2010	20202776
[chemical characterisation of structural components of rahnella aquatilis lipopolysaccharides].	the studies of lipopolysaccharides (lps) of eight rahnella aquatilis strains, isolated from different sources have shown that they contain both s- and r-types of molecules. this fact is evidenced by the presence of high-molecular fraction of o-specific polysaccharides (o-ps) and low-molecular fraction of core oligosaccharides. the predominant monosaccharides of core oligosaccharides were glucose, galactose. the presence of only one high-molecular fraction o-ps was a characteristic feature of all ...	2010	20364711
[comparative characterisation of lipopolysaccharides of rahnella aquatills].	lipopolysaccharides (lps) of eight strains of r. aquatilis isolated from different sources have been studied. the studies of neutral monosaccharide composition evidence that all of lps contain galactose (13.4-68.5%), glucose (5.7-29.8%) and heptose (2.6-8.3%) (depending on strains). some monosaccharides, such as ribose (95u007), rhamnose (95u011, 95u012, 96u036), fucose (95u003, 95u004, 95u007) and mannose (95u012, 96u035, 96u036, 96u037) were absent in lps. arabinose was present in two strains- ...	2009	20455427
rahn-2, a chromosomal extended-spectrum class a beta-lactamase from rahnella aquatilis.	rahnella aquatilis is an environmental enterobacterial species with a chromosomal bla(rahn-1) gene encoding extended-spectrum class a beta-lactamase rahn-1. we describe the diversity of bla(rahn) genes from two groups of strains, g1 and g2, isolated from raw fruits and vegetables, and the new class a beta-lactamase rahn-2.	2010	20511369
comparison of specificity and sensitivity of immunochemical and molecular techniques for determination of clavibacter michiganensis subsp. michiganensis.	detection of clavibacter michiganensis subsp. michiganensis (cmm), causing bacterial canker of tomato, was verified using pta-elisa and ifas with pabs of neogen europe ltd. (uk), and with published and also laboratory-generated pcr primers from the cmm tomatinase gene. the specificity of this technique was determined with 15 plant-pathogenic and 4 common, saprophytic bacteria. with ifas, crossreactions were found for pantoea dispersa, p. agglomerans and rahnella aquatilis, and with pta-elisa for ...	2010	20526836
infection caused by rahnella aquatilis.	the authors report a case of a 27-year-old african american woman with sickle cell disease who developed septic shock caused by a rahnella aquatilis infection associated with a peripherally inserted central venous catheter. the infection was treated successfully with ciprofloxacin. r aquatilis is a gram-negative rod, first isolated from freshwater in 1976, which has been linked to human disease in rare instances, most commonly in immunosuppressed individuals. in addition to this case report, the ...	2010	20545014
production of biogenic amines "in vitro" in relation to the growth phase by enterobacteriaceae species isolated from traditional sausages.	histidine, lysine, ornithine and tyrosine decarboxylase activities were tested in 79 strains of enterobacteriaceae (41 of hafnia alvei, 17 of serratia liquefaciens, 5 of enterobacter cloacae, 4 of citrobacter braakii, 2 of proteus vulgaris, 2 of proteus mirabilis, 2 of providencia stuartii, 2 of klebsiella terrigena, 1 of rahnella aquatilis, 1 of salmonella arizonae, 1 of citrobacter youngae and 1 of escherichia coli) isolated from botillo, a spanish traditional sausage. in general, the strains ...	2010	20673614
identification of cis- and trans-acting elements in phw126, a representative of a novel group of rolling circle plasmids.	phw126, pigrk, pigms31 and prao1 are the only known members of a novel and as yet uncharacterised family of rolling circle plasmids. phw126 contains only two open reading frames, of which one shows homology to pmv158-family mobilisation proteins. here we provide evidence that the second open reading frame encodes a replication protein (rep). mutation or deletion of this gene resulted in replication deficient constructs, but providing functional rep from a compatible vector rescued these construc ...	2011	20854841
occurrence of gram-negative bacteria in hens' eggs depending on their source and storage conditions.	the aim of this study was to analyse the qualitative composition of gram-negative microbes, mainly of the family enterobacteriaceae, including pathogenic bacteria such as salmonella, in the albumens and yolks and on the shells of hens' eggs, depending on their source and on the temperature and duration of their storage. a total of 375 table eggs were studied, from a large-scale poultry farm, a small-scale poultry farm and a supermarket. each group was divided into 5 subgroups according to the te ...	2010	21033566
spoilage-related microbiota associated with chilled beef stored in air or vacuum pack.	in order to study the spoilage-related microbiota of beef at species level, a combination of culture-independent and culture-dependent methods was used to analyse nine different beef samples stored at 4°c in air or in vacuum pack. plate counts on selective agars after 0, 7 and 20 days of storage showed that vacuum packaging reduced the viable counts of brochothrix thermosphacta, pseudomonas spp. and enterobacteriaceae, whereas the growth of lactic acid bacteria (lab) was unaffected. storage in v ...	2010	21056779
genetic and phenotypic diversity of plant growth promoting rhizobacteria isolated from sugarcane plants growing in pakistan.	bacteria were isolated from roots of sugarcane varieties grown in the fields of punjab. they were identified by using api20e/ne bacterial identification kits and from sequences of 16s rrna and amplicons of the cpn60 gene. the majority of bacteria were found to belong to the genera of enterobacter, pseudomonas, and klebsiella, but members of genera azospirillum, rhizobium, rahnella, delftia, caulobacter, pannonibacter, xanthomonas, and stenotrophomonas were also found. the community, however, was ...	2010	21193815
cold-adapted and rhizosphere-competent strain of rahnella sp. with broad-spectrum plant growth-promotion potential.	a phosphate-solubilizing bacterial strain isolated from hippophae rhamnoides rhizosphere was identified as rahnella sp. based on its phenotypic features and 16s rrna gene sequence. the bacterial strain showed the growth characteristics of a cold-adapted psychrotroph, with the multiple plant growth-promoting traits of inorganic and organic phosphate solubilization, 1-aminocyclopropane-1- carboxylate-deaminase activity, ammonia generation, and siderophore production. the strain also produced indol ...	2010	21193830
bacterial and fungal communities of wilted italian ryegrass silage inoculated with and without lactobacillus rhamnosus or lactobacillus buchneri.	to understand the effects of lactic acid bacteria (lab) inoculation on fermentation products, aerobic stability and microbial communities of silage.	2011	21204884
extended-spectrum beta-lactamase gene sequences in gram-negative saprophytes on retail organic and nonorganic spinach.	a substantial proportion of infections caused by drug-resistant gram-negative bacteria (gnb) in community and health care settings are recognized to be caused by evolutionarily related gnb strains. their global spread has been suggested to occur due to human activities, such as food trade and travel. these multidrug-resistant gnb pathogens often harbor mobile drug resistance genes that are highly conserved in their sequences. because they appear across different gnb species, these genes may have ...	2011	21216903
monitoring the bacterial community of maize silage stored in a bunker silo inoculated with enterococcus faecium, lactobacillus plantarum and lactobacillus buchneri.	to monitor variations in the bacterial community and fermentation products of maize silage within and between bunker silos.	2011	21447012
diversity and community structure of culturable arsenic-resistant bacteria across a soil arsenic gradient at an abandoned tungsten-tin mining area.	we studied the bacterial diversity at a single location (the terrubias mine; salamanca province, spain) with a gradient of soil as contamination to test if increasing levels of as would (1) change the preponderant groups of arsenic-resistant bacteria and (2) increase the tolerance thresholds to arsenite [as(iii)] and arsenate [as(v)] of such bacteria. we studied the genetic and taxonomic diversity of culturable arsenic-resistant bacteria by pcr fingerprinting techniques and 16s rrna gene sequenc ...	2011	21724233
freezing and freeze-drying of the bacterium rahnella aquatilis bnm 0523: study of protecting agents, rehydration media and freezing temperatures.	the aim of the present study was to evaluate and compare freezing and freeze-drying treatments for conserving rahnella aquatilis (bnm 0523) with the goal to achieve an adequate commercial formulation of this biocontrol agent.	2011	21913947
Identification of the Midgut Microbiota of An. stephensi and An. maculipennis for Their Application as a Paratransgenic Tool against Malaria.	The midgut microbiota associated with Anopheles stephensi and Anopheles maculipennis (Diptera: Culicidae) was investigated for development of a paratransgenesis-based approach to control malaria transmission in Eastern Mediterranean Region (EMR). Here, we present the results of a polymerase chain reaction (PCR) and biochemical-based approaches to identify the female adult and larvae mosquitoe microbiota of these two major malaria vectors, originated from South Eastern and North of Iran. Plating ...	2011	22163022
exploring new strains of dye-decolorizing bacteria.	this study unveiled a new strategy to explore new indigenous strains with excellent decolorization capabilities from freshwaters and seawaters. two new bacterial decolorizers dx2b and sh7b, which have the capability to decolorize textile dyes, were isolated from cross-strait taiwan and china. according to pcr-augmented 16s rrna gene analyses for strain identification, >99% of nucleotide sequences in isolated strains were identical to type strains rahnella aquatilis, acinetobacter guillouiae, mic ...	2011	22178338
gut-associated bacteria throughout the life cycle of the bark beetle dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) and their cellulolytic activities.	dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) is an endemic economically important insect of the sierra madre occidental in mexico. this bark beetle has an atypical behavior within the genus because just one beetle couple colonizes and kills seedlings and young trees of 11 pine species. in this work, the bacteria associated with the dendroctonus rhizophagus gut were analyzed by culture-dependent and culture-independent methods. analysis of 16s rrna sequences amplified di ...	2012	22234511
maple sap predominant microbial contaminants are correlated with the physicochemical and sensorial properties of maple syrup.	maple sap processing and microbial contamination are significant aspects that affect maple syrup quality. in this study, two sample sets from 2005 and 2008 were used to assess the maple syrup quality variation and its relationship to microbial populations, with respect to processing, production site and harvesting period. the abundance of maple sap predominant bacteria (pseudomonas fluorescens group and two subgroups, rahnella spp., janthinobacterium spp., leuconostoc mesenteroides) and yeast (m ...	2011	22236761
occurrence of non-lactic acid bacteria populations involved in protein hydrolysis of cold-stored high moisture mozzarella cheese.	the aim of this study was to analyse non-lactic acid bacteria populations (nlabps) and evaluate their role in proteolysis of cold-stored high moisture (hm) mozzarella cheese. nlabps reached values close to 8 log cfu ml(-1) after seven days of cold storage. sequencing of 16 rdna and rpob genes and molecular biotyping allowed to identify 66 bacterial strains belonging to 25 species from 15 genera, mainly represented by pseudomonas, acinetobacter, and rahnella. fifteen strains showed proteolytic ac ...	2012	22265281