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[laboratory study on measuring the insecticidal activity, on triatoma infestans, of lindane stored for 20 years]. 20132435299
critical electrolyte concentration of the heterochromatin and euchromatin of triatoma infestans.the binding of toluidine blue molecules under mg2+ competitive staining conditions was investigated in chromocentres and the euchromatin of single- and multi-chromocentred nuclei of triatoma infestans malpighian tubule cells. it was demonstrated that the chromocentre of single-chromocentred nuclei exhibited the largest critical electrolyte concentration (cec) value (0.4 m), followed by the chromocentres of multi-chromocentred nuclei (0.3 m) and the euchromatin (0.2 m). the differences in cec val ...19892480868
myocardial involvement in cavia porcellus naturally infected with trypanosoma cruzi.this paper describes the myocardial involvement analyzed by histopathological examination in rural cavia porcellus during natural t. cruzi infection. four cavia porcellus of both sexes were bred in a house free of triatoma infestans. in contrast, four animals were born and lived in a rural yard colonized by t. infestans. autopsies were performed at 6-9 months of age, in animals weighing 550 to 750 grams. the naturally infected cavia porcellus presented moderate and severe lymphocyte and plasmocy ...19892487739
darwin's illness.darwin's chronic ill health began after 1838. by his own account he had been exposed to triatoma infestans, a vector of chagas' disease, and his symptoms were compatible with chagas' disease. the case for rejecting a diagnosis of chagas' disease is reviewed; however, the weight of evidence favors this diagnosis, as originally suggested by saul adler.19892496051
cannibalism and coprophagy are modes of transmission of blastocrithidia triatomae (trypanosomatidae) between triatomines.transovarial transmission was not detectable among blastocrithidia triatomae-infected triatoma infestans. rather, b. triatomae was transmitted directly between triatomines by cannibalism and coprophagy. cannibalism conditions that excluded coprophagy always resulted in an infection of dipetalogaster maxima. the efficiency of transmission was not influenced by the blood source--mice or chickens--fed to the infected donor bugs although chicken blood lyses the epimastigotes of the stomach populatio ...19892498511
cultivation of blastocrithidia triatomae (trypanosomatidae) on a cell line of its host triatoma infestans (reduviidae).blastocrithidia triatomae parasitizes vectors of chagas' disease and is very difficult to cultivate in conventional media. however, co-cultivation with a cell line of its host triatoma infestans (ti-32; in schneider's drosophila medium supplemented with 20% foetal calf serum and 10% tryptose phosphate broth) led to vigorous growth at 24 or 28 degrees c without an adaptation phase. more than 60 primary cultures were initiated successfully without any failures. subcultures could be started immedia ...19892505214
parasite/host-interrelationships of the trypanosomatids trypanosoma cruzi and blastocrithidia triatomae and the reduviid bug triatoma infestans: influence of starvation of the bug.we have investigated the effects of trypanosoma cruzi and blastocrithidia triatomae on the life span of starved second/third (l2/3), third/fourth (l3/4), and fourth/fifth instars (l4/5) of triatoma infestans, which had been infected in the first instar and fed last in the second, third or fourth instar respectively. the intestines and sometimes also the flagellate populations of dead bugs were examined. in uninfected l2/3 a higher relative humidity prolonged the life span. with a maximal surviva ...19892513786
[isolation of trypanosoma cruzi samples by xenodiagnosis and hemoculture from patients with chronic chagas' disease].fifty nine chronic chagasic patients were simultaneously submitted to xenodiagnosis and hemoculture for trypanosoma cruzi samples isolations. the xenodiagnosis was done with 40 panstrongylus megistus, triatoma infestans and dipetalogaster maximus nymphs, performing 120 triatomines. groups of 10 insects per specie were dissecated and the intestinal content pooled and examined, after previous trituration and homogenization. the microscopically negative material was seed into lit medium and examine ...19892517566
[parasitemia in chronic chagasic patients evaluated using the index of infected triatoma in xenodiagnosis].as part of a pre-treatment study of chronic chagas infections, the parasitemia of 206 patients (85 men and 121 women, aged 7 to 80 y) from virgem da lapa, minas gerais state brazil, was evaluated by three xenodiagnoses per patient during a one year period. each time, 40 3rd or 4th instar nymphs of triatoma infestans were applied. the parasitemia was arbitrarily classified as: not detected (when all nymphs were negative), low (when the number of infected nymphs was less than 2%), medium (when it ...20062517802
population dynamics of triatoma infestans under natural climatic conditions in the argentine chaco.using experimental chicken houses at a site in central argentina where the bug triatoma infestans (klug) is endemic, nine populations of this vector of chagas disease were monitored during a 34-month period. bug populations with four chickens as hosts were consistently larger than those with two chickens as hosts. age structure of the bug population followed a similar pattern irrespective of the initial age structure. egg to adult mortality was consistently around 98.5% and there was no consiste ...19892519662
[seroprevalence of antibodies against trypanosoma cruzi in 13 departments of uruguay].in 1985 a study was undertaken of the prevalence of trypanosoma cruzi antibodies in 13 departments of uruguay where transmission of the parasite by the vector triatoma infestans persists. a total of 5,924 serum samples were selected using a probabilistic method--3,840 from individuals over the age of 12 (sample i) and 2,084 from subjects who were 12 years old (sample ii). the population was classified according to place of residence (capital city, non-capital city, suburban area, and rural area) ...19892529870
binding of lectin-gold conjugates by two trypanosoma cruzi strains in ampullae and rectum of triatoma infestans.the ampullae and recta of triatoma infestans infected with trypanosoma cruzi were incubated with lectin-gold conjugates of wheat germ agglutinin (wga) and soy bean agglutinin (sba) and investigated by electron microscopy. t. cruzi colonized the whole lumen of the ampullae. the rectal pads with their intensive intracellular membrane system and thin cuticle were often covered by a compact layer of flagellates, whereas the remaining rectal sac- recognizable by a thick layered cuticle - was colonize ...19892575865
trypanosoma cruzi: quantitative studies of development of two strains in small intestine and rectum of the vector triatoma infestans.this paper describes the development stages and numbers of flagellates of two strains of trypanosoma cruzi living in the small intestine and rectum of the insect, triatoma infestans, during the first 12 weeks postinfection (pi). mainly epimastigotes and occasionally amastigotes and final trypomastigotes developed in the small intestine but after starvation periods of 3 or 4 weeks higher percentages of spheromastigotes including their transitional forms to/from epimastigotes were found. in the re ...19892649388
purification and properties of the very high density lipoprotein from the hemolymph of adult triatoma infestans.the very high density lipoprotein (vhdl) of triatoma infestans hemolymph from adult males has been isolated and purified by two-step density gradient ultracentrifugation. it appears to be homogeneous as judged by native polyacrylamide gel electrophoresis. the content of vhdl in hemolymph was estimated to be 8 mg protein/ml. the purified protein has a molecular weight (mr) of 450,000, is composed of six subunits of mr approximately equal to 77,000, and possesses a high content of aromatic amino a ...19892677201
fatty acid biosynthesis in the integument tissue of triatoma infestans.1. the biosynthesis of lipids and their distribution in several tissues were investigated by injection of 1-14c acetate in females and 5th instar nymphs of the hematophagous hemiptera t. infestans. 2. biosynthesis of palmitic, palmitoleic, stearic, oleic and very long chain fatty acids up to 26 carbons and hydrocarbons, was shown. they were found in haemolymph, fat body, integument, epicuticle and oocytes with special distribution. 3. epicuticular hydrocarbon labelling was shown to precede that ...19892680250
critical electrolyte concentration of heterochromatin and euchromatin in cells of starved animals.the critical electrolyte concentration (cec) of chromatin, considered to be the salt concentration at which the phenomenon of metachromasy is completely abolished, when using toluidine blue as the cationic dye and mg2+ ions as inorganic cations, was determined for the euchromatin and chromocentral heterochromatin of cells from starved animals. the objective was to determine variations in cec induced by the starvation stress, which could be related to structural alterations and/or to the composit ...19892699727
trypanosoma cruzi isolates from argentina and chile grouped with the aid of dna probes.fifty-two isolates and several clones from trypanosoma cruzi, the agent of chagas' disease, were analyzed using cloned minicircles or total kinetoplast dna as probes. isolates were obtained from triatomines, guinea pigs and infected humans in the central and northern regions of argentina and the north of chile. 35% of all the randomly selected isolates could be identified with one cloned minicircle probe. this widely distributed t. cruzi group was detected on both sides of the andes mountain ran ...19872823134
effect of vector on infectivity of trypanosoma cruzi.a comparative study was carried out on the interaction between triatoma infestans and bloodstream forms (bsf) of trypanosoma cruzi isolates ra and up, both lethal for mouse, and ca-i nonlethal for this host. parasite duplication was readily detected in triatomes fed with ca-i, metacyclic (mtc) differentiation reaching a maximum at an optimum ingestion level of 250 bsf/insect. progress and differentiation proved much lower for ra, but reached intermediate values for the up isolate. assays for inf ...19852862777
colonization of the rectum of triatoma infestans by trypanosoma cruzi: influence of starvation studied by scanning electron microscopy.the colonization of the different regions of the rectum of triatoma infestans by a trypanosoma cruzi strain (zymodeme i) originating from the same locality as the bugs was studied by scanning electron microscopy after different periods of starvation of the bugs. throughout the first 16 weeks no changes in colonization pattern could be observed. parasite density was always minimal at the midgut/rectal junction and highest on the rectal pads; it was at a similar level in the other three regions of ...19862882662
activity of the insect growth regulator fenoxycarb (ro-13-5223) on triatoma infestans (hemiptera).1. fenoxycarb (ro-13-5223) exhibits a strong juvenile hormone type of activity against triatoma infestans. 2. topical treatment of last-stadium nymph with the juvenoid caused the formation of superlarvae, adult-larvae intermediates and adultoids. the ed50 was 0.02 microgram/insect. 3. taking into account abnormalities in oceli, head, thorax, legs, wings, abdomen, conexivo and external genitalia, a scoring system to evaluate the retention of juvenile characters after moulting was developed. 4. in ...19872888581
direct transmission of trypanosoma cruzi between vectors of chagas' disease.trypanosoma cruzi was transmitted directly between triatomines by cannibalism or coprophagy. different conditions involving cannibalism that excluded coprophagy were studied in dipetalogaster maximus. infections occurred if an uninfected donor bug sucked infectious blood and if this blood was taken up from the stomach by a cannibalistic bug. if the donor was infected and sucked uninfected blood afterwards, the source of the uninfected blood determined the transmission rate: if the uninfected blo ...19882896441
[biochemistry of the developmental cycle of triatoma infestans. vii. lipid composition of the cuticle surface extracted with hexane].the lipid composition of triatoma infestans (vinchuca) epicuticle was studied. the insect lipids were extracted with hexane and fractionated by chromatography. long chain fatty alcohols, hydrocarbons, waxes, triacylglycerols, diacylglycerols, free fatty acids, cholesterol, cholesterol esters and monoacylglycerols were detected. the composition of fatty acids, hydrocarbons and alcohols was studied by gas-liquid chromatography and mass spectrography. linear and branched hydrocarbons were separated ...19852938407
[biochemistry of the growth cycle of triatoma infestans (vinchuca). viii. preliminary study of hemo-lymphatic apolipoproteins in the adult male].the three lipoproteins of high (hdl) and very high density (vhdl-i and vhdl-ii) were separated from the hemolymph of adult males of t. infestans. they were delipidated and the corresponding apolipoproteins examined by sodium dodecyl sulphate (sds) gel electrophoresis in polyacrylamide in the presence of 2-mercaptoethanol. they were also iso-electro focused in a ph gradient of 5 to 8. all three groups of apolipoproteins were separated in several polypeptide chains, but all of them had in common a ...19852938415
[biochemistry of the evolutive cycle of triatoma infestans. ix. composition of cuticular hydrocarbons compared to other triatominae].the hydrocarbon composition of epicuticular lipids of different species of triatomini were determined and compared by gas-liquid chromatography. t. infestans, t. delpontei, t. rubrovaria, t. sordida, t. guasayana and t. vitticeps from infestans subgroup and t. pallidipennis from rubrofasciata, were studied. all these species had in common the same kind of hydrocarbons. the major components were n c29; n c31; 12+14+15+18 methyl c38; 15, 19+14, 18 dimethyl c39; 12+14+17+19 methyl c36 and 4 methyl ...19862945401
trypanosoma cruzi: differentiation to metacyclic trypomastigotes in the presence of adp-ribosyltransferase inhibitors.the participation of adp-ribosyltransferase in trypanosoma cruzi differentiation to the metacyclic stage was evaluated by analyzing morphogenesis blockage by specific enzyme inhibitors: benzamide, 3-aminobenzamide, theophylline, and nicotinamide. in vitro assays showed a statistically significant reduction in the number of metacyclic forms only when any one of the four inhibitors was added during the period of interaction between epimastigote and triatoma infestans intestinal homogenate or when ...19872960557
dispersal flight by triatoma infestans in an arid area of argentina. 19882980200
trypanosoma cruzi: differentiation after interaction of epimastigotes and triatoma infestans intestinal homogenate.incubation of trypanosoma cruzi epimastigotes with triatoma infestans intestinal homogenate leads to differentiation to the metacyclic trypomastigote. features of this interaction are presented. the morphogenetic mechanism was triggered almost at once; for the minimum interaction period assayed (15 min), the degree of differentiation achieved in grace medium by day 6 was 70.0 +/- 9.0%. longer interaction periods failed to improve differentiation. the morphogenesis became irreversible at 4 hr aft ...19863023131
developmental time and mortality of larvae of triatoma infestans infected with trypanosoma cruzi.triatoma infestans were infected with trypanosoma cruzi (zymodeme 1) at the first feed after eclosion from the egg; interstadial development times and mortality rates were then recorded until the imaginal moult and compared with those of uninfected controls. no retardation of development occurred in infected bugs and their mean total mortality rate (9%) was only slightly higher than that (6%) of uninfected controls (due solely to 4 additional deaths). this is the first demonstration, under optim ...19883051552
inhibition of xanthine dehydrogenase from triatoma infestans by some purine analogues.xanthine dehydrogenase (ec 1.2.1.37) activity was determined in the fat body of the chagas' disease vector triatoma infestans using a system in which phenazine methosulphate was associated with p-iodonitrotetrazolium violet as electron acceptors for the oxidation of the substrate xanthine. under the standard conditions used, xanthine was the substrate of choice. the apparent michaelis-menten constant (km) for xanthine was found to be 0.217 +/- 0.020 mm (mean +/- standard deviation of four indepe ...19883052441
characterization of triatoma virus, a picorna-like virus isolated from the triatomine bug triatoma infestans.some properties of triatoma virus (trv), a picorna-like virus recently isolated from triatoma infestans, have been studied. electron microscopic observations of purified viral preparations showed the presence of non-enveloped viral particles 30 nm in diameter. the sedimentation coefficient of virus particles was about 165s and the buoyant density in cscl was 1.39 g/ml. the viral genome was composed of one single-stranded rna molecule with an mr of 3 x 10(6). three major polypeptides with mr valu ...19883053988
development of isolated and group-reared first instars of triatoma infestans infected with trypanosoma cruzi. 19883057492
transport and utilization of free fatty acids in triatoma infestans.triatoma infestans hemolymph has 0.31 mg/ml of free fatty acids and 2.8 mg/ml of diacylglycerols. almost all the diacylglycerols are transported by lipophorin whereas free fatty acids are carried by lipophorin and a very high density lipoprotein. the binding of cis-parinaric acid to lipophorin was employed to specify the free fatty acid binding properties of lipophorin. lipophorin has 10 binding sites of high affinity (3 x 10(7)) and approximately 45 binding sites of low affinity (1 x 10(6)). th ...20113060117
cardiac morbidity due to chagas' disease in a rural community in bolivia.the prevalence of cardiac morbidity due to chagas' disease was assessed in a rural community in central bolivia. sixty-nine of 104 persons (66%) were seropositive to trypanosoma cruzi by two serological methods. two of 35 (6%) seronegative individuals presented with modest ecg alterations (left anterior hemiblock), but 21 of 69 (30%) seropositives showed modest and severe abnormalities (6 complete right bundle branch block, 2 polyfocal or frequent extrasystoles, 9 ischaemic st alterations). a hi ...19883063552
junctional specializations in the seminiferous epithelium of an insect (triatoma infestans): a freeze fracture and lanthanum tracer study.intercellular junctions in the seminiferous epithelium of the testis of triatoma infestans were examined by conventional electron microscopy, tannic acid fixation, electron-opaque tracers, and freeze-fracture techniques. distinctive aspects of the intercellular junctions are described in different regions of the testis follicles. in the basal region, close to the basal lamina, smooth septate junctions intermingled with gap junctions were observed between sustentacular cells. in the parabasal reg ...19883070831
studies on the trehalase from the hemolymph of triatoma infestans. 19883071834
metacyclogenesis of trypanosoma cruzi in the vector triatoma infestans. 19883075693
trypanosoma cruzi and trypanosoma rangeli in saimiri sciureus from bolivia and saguinus mistax from brazil.examination of blood films for trypanosomes in primates housed at the tulane university delta regional primate research center showed that 47% (32/68) of the bolivian saimiri sciureus and 51% (68/135) of brazilian saguinus mistax harbored one or more types: trypanosoma (schizotrypanum) cruzi in 6% to 7% and trypanosoma (herpetosoma) spp. or trypanosoma (megatrypanum) spp. in 39% and 45%, respectively. trypanosomes were isolated from a sample of the infected monkeys and morphobiological studies w ...19863082228
lability to acid hydrolysis in some different dna-protein complexes of spermatozoa.the feulgen hydrolysis kinetics was investigated in spermatozoa with different composition in dna-protein complexes. the species used were: bos taurus (arginine rich nuclear protein also containing cystine residues), pichroplus bergi, triatoma infestans (arginine-rich nuclear protein), lytechinus variegatus and apis mellifera (lysine-rich nuclear protein). the spermatozoa were subjected to feulgen's reaction, after varying the fixative type and the hydrolysis times. feulgen-dna values were obtai ...19863088898
investigation of hycanthone binding to dna in chromatin with different supra-organization, composition, and function.hycanthone is a drug used for the clinical treatment of schistosomiasis, although inducing chromosomes breaks and mutagenesis when it enters the dna double helix. based on visual observation of characteristics of basophilia and anisotropy after toluidine blue staining, a preferential binding of hycanthone to heterochromatin could be demonstrated for the nuclei of the malpighian tubules of triatoma infestans. in other cellular systems like cattle kidney cells in culture, plant cells, and mouse ly ...19863090842
an outbreak of chagas' disease in southwestern bahia, brazil.an outbreak of 20 cases of acute chagas' disease followed the movement of triatoma infestans into the county of riacho de santana, bahia, brazil. the outbreak was unusual in that the majority of cases occurred in adults. vector control measures were implemented. three years after the outbreak, a rural community was examined to determine the extent of human infection and disease due to trypanosoma cruzi. ninety of 440 residents (20.5%) had serologic evidence of infection, but rates of electrocard ...19863094393
vitellin and vitellogenin characterization of triatoma infestans and related species.the vitellogenin-vitellin (vg-vn) of triatoma infestans is a glycolipoprotein with a mol. wt. of 220,000; is a high density lipoprotein (1.18-1.21 g/ml) with low electrophoretic mobility at ph 8.2. there are many common proteins present in females, males, nymphs and eggs, but none is a glycolipoprotein. the major band of protein revealed a mol. wt. of 43,000, it is an anodic protein which appears in all the fractions of saline gradient. males and females have a glycolipoprotein with electrophore ...19863111174
epidemiology of chagas' disease in northern chile: isozyme profiles of trypanosoma cruzi from domestic and sylvatic transmission cycles and their association with cardiopathy.trypanosoma cruzi was isolated from 98 patients, 59 triatoma infestans, 51 triatoma spinolai, and 1 octodon degus from northern chile. with few exceptions, stocks originating from domestic hosts were classified, based on their isozyme profile, as principal zymodeme (z)2, while sylvatic stocks from t. spinolai and the rodent o. degus showed z1 profiles. these results indicate the existence of separate domestic and sylvatic transmission cycles. clinical data and t. cruzi isozyme profiles from 107 ...19873116867
the occurrence of arachidonic acid in triatoma infestans.1. gas-liquid chromatography and mass spectrometry were used to verify the presence of arachidonic acid (20:4 omega 6) in adult male and female organs of the blood-suckling bug triatoma infestans. fat body, gonad and head lipids were analyzed. 2. male gonads contained the highest percentage of phospholipids and the highest percentage of arachidonic acid. 3. phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and sphingomyelin were isolated from gonads and head ...19873117486
differences in binding of lectin-gold conjugates by trypanosoma cruzi and blastocrithidia triatomae (trypanosomatidae) in the intestine of triatoma infestans (reduviidae).two species of trypanosomatid flagellates, trypanosoma cruzi and blastocrithidia triatomae which develop in the intestine of triatoma infestans, were incubated with lectin-gold conjugates to demonstrate sugar components of the cell surface with the electron microscope. t. cruzi, strain "chile 5" (zymodeme 1), bound sba-gold, revealing n-acetyl-galactosamine and/or galactose residues. wga-bsa-gold, specific for n-acetylglucosamine, did not bind to the surface of this flagellate. on the other hand ...19873125542
dynamics of transmission of trypanosoma cruzi in a rural area of argentina: ii. household infection patterns among children and dogs relative to the density of infected triatoma infestans. 19873126846
developmental time and mortality in larvae of the reduviid bugs triatoma infestans and rhodnius prolixus after coprophagic infection with blastocrithidia triatomae (trypanosomatidae). 19883127477
the influence of blastocrithidia triatomae (trypanosomatidae) on the reduviid bug triatoma infestans: in vivo and in vitro diuresis and production of diuretic hormone.the homoxenous flagellate blastocrithidia triatomae disturbs excretion in triatoma infestans. during the first 4 and 24 h after feeding, infected 5th instars excreted approximately a 2.5-fold smaller volume of urine. consequently, about double the normal volume of haemolymph could be obtained from these infected bugs 24 h after feeding. surprisingly, the in vitro secretion rates of malpighian tubules from uninfected and infected bugs were nearly identical. in addition, the storage and release of ...19883129692
suitability of in-vitro xenodiagnosis: development of trypanosoma cruzi in triatoma infestans depending on larval stage of bugs and number of trypomastigotes taken during in-vitro blood meal.a study on artificial xenodiagnosis was carried out for the identification of t. cruzi, the causative organism of the american trypanosomiasis (chagas disease) using the natural vector t. infestans. it was investigated whether one single t. cruzi (strain b) is sufficient for successful infection of t. infestans. since the infection took place by artificial feeding, the average amount of blood taken by larval stages i-v in one meal was determined. on the basis of these results the feeding solutio ...19883134769
influence of blastocrithidia triatomae (trypanosomatidae) on the reduviid bug triatoma infestans: alterations in the malpighian tubules.to investigate the cellular basis of our recent observation that the trypanosome blastocrithidia triatomae disturbs excretion in triatoma infestans, we compared the morphology and ultrastructure of malpighian tubules in infected and noninfected bugs. tubules of bugs infected 3-4 months before dissection did not show any morphological alterations in either the upper or lower regions. in fifth instars with a long lasting infection (9-10 months) and a correspondingly retarded larval development, th ...19883148931
trypanosoma cruzi: origin of metacyclic trypomastigotes in the urine of the vector triatoma infestans.population density and percentage of the different stages of an established infection of trypanosoma cruzi were determined for two parts of the excretory system and for the rectum of fifth instars of triatoma infestans unfed and 4 hr after feeding. these data were also evaluated for feces and urine of the fed bugs. in the first unfed group only small populations of the flagellate occurred in the malpighian tubules and ampullae and not in all bugs. the three rectal populations (rectal lumen and a ...19883280333
lipid thermotropic transitions in triatoma infestans lipophorin.the structure and lipid thermotropic transitions of highly purified lipophorin of triatoma infestans were examined by several techniques: steady-state fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (dph), cis-parinaric acid (cis-pna) and trans-parinaric acid (trans-pna), light scattering fluorescence energy transfer between the lipophorin tryptophan residues and the bound chromophores, dph, trans-parinaric acid cis-parinaric acid, gel electrophoresis, and gel filtration. fluorescence ...19883284956
[biomorphogenetic changes caused by the application of precocene ii in nymphs of triatoma infestans (klug, 1834) (hemiptera, reduviidae, triatominae)].the morphogenetic effect of precocene ii on 4th instar nymphs of triatoma infestans was examined. topical treatment with precocene ii (200, 300 and 400 micrograms/nymph) induced the formation of adultoids with the following characteristics: rudimentary wings, three segmented tarsi, ocelli, deformed genitalia and mouth-parts and testes with intermediary development. precocene ii also increases the duration of the molting cycle. the survival of the adultoid insects was related to the inverse of th ...19863295468
[experimental infection of triatoma infestans (hemiptera: reduviidae) with leptospira icterohaemorrhagiae and its transmission to laboratory mammals]. 19863296076
the surface extracellular coat of the midgut in triatoma infestans. i. mechanism of development.the mechanism of formation of the apical surface, extracellular coat, and microvilli of the midgut of adult triatoma infestans (hematophagous insect) has been studied with the electron microscope after a recent meal and after 10 days postfeeding. in the first case the extracellular coat becomes disorganized and mixed with the intestinal content. at the same time, small basal cells show the appearance of intracytoplasmic lipid droplets surrounded by a well-developed rough endoplasmic reticulum; t ...20063302058
a comparative study of trypanosoma cruzi enzyme polymorphism in south america.1. the electrophoretic patterns of 13 loci were used to compare the three main t. cruzi zymodemes (z) circulating in north/northeastern brazil (br 1 through 3) with the four principal zymodemes present in southeastern (bra through d) brazil. 2. nei's standard genetic distances (d) obtained by paired comparison of the brazilian zymodemes indicate the presence of four divergent groups, separated by d = 1.18. zymodemes brb and brd presented heterozygous patterns for six and three loci, respectively ...19863307956
colonization of the rectum of triatoma infestans by trypanosoma cruzi studied by scanning electron microscopy: influence of blood uptake by the bug.the colonization of the different regions of the rectum of triatoma infestans by trypanosoma cruzi was studied in unfed larvae, during and after feeding of the bug by scanning electron microscopy. the rectal pads always possessed the highest population densities, but in some bugs the main rectal sac and the region around the anus were also covered by a "carpet" of flagellates. such high densities were never observed at the midgut/rectal junction. a slight decrease in this region might be caused ...19873309941
recombination nodules, synaptonemal complexes and heterochromatin in the hemipteran triatoma infestans. 19873332298
polyploidization of the btc-32 cell line from triatoma infestans (hemiptera, reduviidae).the cytophotometric evaluation of the feulgen-dna content of the btc-32 cells at passage 160 after 6 days of growth demonstrated that polyploidy is relatively frequent in this cell line. 4c values were assumed to pertain to diploid nuclei at the s or g2 phases but also to polyploid nuclei at the g1 phase. polyploidy in 4c nuclei is assumed to be attained by endomitosis. however, there are morphological indications that polyploidization in cells with an 8c-128c feulgen-dna content could result fr ...19873333490
[home ecology of trypanosomiasis americana: feeding profile of triatoma infestans in a rural area of the santiago del estero province, argentina]. 19873333976
an improved procedure for the purification of trypanosoma cruzi (chagas, 1909) metacyclics from the insect vector.we have developed an improved procedure for isolating and purifying the metacyclic trypomastigote form of trypanosoma cruzi from infected triatoma infestans. the procedure was simple, did not require time-consuming removal of the insect gut, and gave a good recovery of metacyclics. purification involved centrifugal flotation of the parasites in percoll followed by diethylaminoethyl cellulose column chromatography. the resulting purified metacyclics exhibited no loss of infectivity when assayed i ...19863514902
trypanosoma cruzi morphogenesis: preliminary purification of an active fraction from hemolymph and intestinal homogenate of triatoma infestans. 19863528446
isozyme evidence of lack of speciation between wild and domestic triatoma infestans (heteroptera: reduviidae) in bolivia. 19873546693
small nonoccluded viruses from triatomine bug triatoma infestans (hemiptera: reduviidae). 19873549911
[biochemistry of the development cycle of triatoma infestans (vinchuca). x. hemolymph lipoproteins of females].a lipoprotein of high density (hdl) and three of very high density (vhdl-i, vhdl-f and vhdl-ii) were separated by ultracentrifugation from t. infestans female hemolymph. lipids were mainly transported by hdl, whereas vhdl-ii presented the highest protein content. in all the lipoproteins, 1,2 and 1,3-diacylglycerols, triacylglycerols, free fatty acids, cholesterol, cholesterol esters and hydrocarbons, were present. phosphatidylcholine and phosphatidylethanolamine were also identified. the main li ...20123554896
theoretical approach to active dispersal and colonization of houses by triatoma infestans.dispersive flight by triatoma infestans was modelled by assuming either unidirectional flight in a straight line, or that flight followed a pattern of 'random walk' analogous to two-dimensional brownian motion. parameter estimates for the models were derived from previous fieldwork in argentina and brazil. calculated probabilities of houses becoming infested over a 1 year period were compared with actual changes of house infestation in a small farming community in central brazil. it was found th ...19853906147
[study of 11 enzymes and formal genetic findings for 19 enzymatic loci in triatoma infestans (hemiptera: reduviidae)]. 19853907524
population dynamics and control of triatoma infestans. 19853931580
[isoenzymatic studies of the principal vector of chagas disease: triatoma infestans (hemiptera: reduviidae)]. 19853931581
[fenitrothion (oms-43) in the control of triatoma infestans, the vector of chagas disease in argentina]. 19853931583
chagas' disease in pre-columbian south america.the quest for the origin and dispersion of chagas' disease, the second most important vector-borne disease in latin america, has epidemiological, immunological, and genetical implications. conjectures based on accounts of chroniclers, reviews of the archaeological literature and the present distribution of triatomine bugs, the vectors of the disease, held that the origin of the adaptation of triatoma infestans (a species of the subfamily triatominae) to human dwellings occurred in prehistoric ti ...19853936363
[preliminary field tests to measure the residual action of hexachlorocyclohexane on triatoma infestans nymphs]. 19724122725
[spraying suspension in the control of triatoma infestans in areas of the state of são paulo, brazil]. 19694190315
the induction of nad kinase by ddt in triatoma infestans. 19674291968
some properties of the microsomal system metabolizing ddt in triatoma infestans. 19694389231
vital statistics of triatominae (hemiptera: reduviidae) under laboratory conditions. i. triatoma infestans klug. 19724559948
antigenic components in triatoma infestans haemolymph. 20154561728
[ultrastructure of the egg of triatoma infestans klug (heteroptera, reduviidae), morphogenesis of embryonic cuticles and the significance of the envelopes in the transit of water]. 19734572462
[anomalous forms of triatoma infestans]. 19724574397
female specific proteins in triatoma infestans haemolymph. 19734590007
[opening mechanism of the mouth of the hematophagous hemipteran, triatoma infestans]. 19744590239
[comparative yield of two methods of rearing triatoma infestans (author's transl)]. 20004596481
notes on colonization of triatoma infestans (hemiptera, reduviidae). 19724599489
[sensitivity of the bug triatoma infestans klug. to dieldrin in relation to its stage of development and physiological condition]. 20134615261
cytogenetics of triatominae: ii-spermatogenesis in hybrid between triatoma infestans and triatoma pseudomaculata (hemiptera, reduviidae) 19744618618
[action of 7 insecticide preparations on laboratory-raised triatoma infestans]. 19724628950
laboratory studies of trypanosoma cruzi infections in: rhodnius prolixus--larvae and adults in: triatoma infestans, t. protracta and t. maculata--adults. 19674861087
glutathione turnover in triatoma infestans treated with 2,2-bis-(p-chlorophenyl)-1,1,1-trichloroethane. 19674864257
effect of ddt on incorporation of c14-labeled glucose into protein and soluble intermediates of nymphal triatoma infestans. 20164866860
[observations on the dieldrin sensitivity of larvae of triatoma infestans under various nutritional conditions]. 19664866994
[method for growing triatoma infestans (klug, 1834) (hemiptera, reduviidae) in the laboratory]. 19674877507
response of single olfactory cell of triatoma infestans to human breath. 19684881008
[on the discovery of triatoma infestans (klug) in pernambuco]. 20084881101
[structure of the female reproductive system of triatomid bugs, rhodnius prolixus and triatoma infestans, and determination of their physiological age]. 20164885880
[gonotrophic relations in triatomid bugs rhodnius prolixus and triatoma infestans]. 20174886727
[follicular degeneration in triatomid bugs, rhodnius prolixus and triatoma infestans]. 20174886734
[the effect of blood meal size taken in the nymphal stage on moulting into imago in the triatomid bugs. rhodnius prolixus and triatoma infestans]. 20174894411
[problems due to reinfection by triatoma infestans in disinfected dwellings and the concept of reinfection]. 19694904048
[variations of hemolymph of triatoma infestans. report]. 19694906465
[monthly variations of the incidence of the evolution forms of triatoma infestans and panstrongylus megistus in the community of bambui, state of minas gerais (ii: 1951-1964)]. 19684908873
blastocrithidia triatomae n. sp. found in triatoma infestans from argentina. 19714943630
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