Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| comparison of culture-confirmed erythema migrans caused by borrelia burgdorferi sensu stricto in new york state and by borrelia afzelii in slovenia. | the clinical manifestations of lyme borreliosis in north america and europe seem to differ, but a systematic comparison has never been done. | 1999 | 9890847 |
| [borrelia burgdorferi, taxonomy, pathogenicity and spread]. | borrelia burgdorferi sensu lato is a bacterial complex of at least 10 species, most of which are non pathogenic for humans. non pathogenic species seem to be more vector-specific, their distribution being restricted to that of this vector. in opposition, the three species pathogenic for humans, borrelia burgdorferi sensu stricto (b.b.s.s.), borrelia garinii (b.g.), and borrelia afzelii (b.a.), are transmitted by several species of ticks more diversified in their host choice. each of these three ... | 1998 | 9921400 |
| transmission of borrelia afzelii from apodemus mice and clethrionomys voles to ixodes ricinus ticks: differential transmission pattern and overwintering maintenance. | this study deals with the ecology of lyme borreliosis in europe. the relationships between borrelia burgdorferi sensu lato spirochetes, clethrionomys and apodemus rodent reservoirs and the ixodes ricinus tick vector were investigated during 16 consecutive months in an enzootic area in switzerland. cultivation of ear skin biopsies was used to isolate spirochetes from c. glareolus, a. sylvaticus, a. flavicollis and glis glis. borrelia infection was more frequently observed in clethrionomys than in ... | 1999 | 10070659 |
| diagnostic detection and direct genotyping of borrelia burgdor feri regular by polymerase chain reaction in cerebrospinal fluid in lyme neuroborreliosis. | background: a dna target imbalance in favor of the plasmid-borne outer surface protein a (ospa) versus chromosomal genes has been thought to explain the relatively high diagnostic sensitivity of an ospa-based polymerase chain reaction (pcr) on joint fluid from patients with lyme arthritis. the aim of this study was to evaluate the diagnostic sensitivity of the ospa-based pcr on cerebrospinal fluid (csf) samples from patients with lyme neuroborreliosis and to perform dna sequence analysis on the ... | 1998 | 10089270 |
| isolation of borrelia afzelii from circumscribed scleroderma. | a 45-year-old man presented with circumscribed scleroderma (cs) on the extremities. histology of lesional skin showed the typical manifestations of scleroderma including a perivascular and interstitial infiltrate of lymphocytes and plasma cells; in one of the biopsies spirochaetes could be detected. despite treatment with penicillin, progression of cs was observed and spirochaetes were isolated from skin cultures obtained from active scleroderma lesions. these spirochaetes were identified as bor ... | 1999 | 10354035 |
| sexual transmission of borrelia garinii by male ixodes persulcatus ticks (acari, ixodidae). | we investigated the transmission of borrelia garinii and borrelia afzelii between male and female ixodes persulcatus ticks. for this purpose the infection rate of partners from tick couples was determined by polymerase chain reaction and reverse line blot. in couples, where the male tick was infected with b. garinii, four out of nine female partners carried b. garinii. in eight couples, male ticks had a dual infection of b. afzelii and b. garinii and three female partners were infected by borrel ... | 1999 | 10356767 |
| density of deer in relation to the prevalence of borrelia burgdorferi s.l. in ixodes ricinus nymphs in rambouillet forest, france. | the rambouillet forest, a lyme disease-endemic area near paris, france, was surveyed from september 1994 to october 1995 to determine the risk periods and zones for humans. firstly, during the period of ixodes ricinus activity, abundance of nymphs is greater in spring than in autumn. secondly, we observed significant variation in nymphal abundance between zones according to the density of cervids. the polymerase chain reaction (pcr) was used to detect dna of borrelia burgdorferi sensu lato in 46 ... | 1999 | 10356769 |
| osp17, a novel immunodominant outer surface protein of borrelia afzelii: recombinant expression in escherichia coli and its use as a diagnostic antigen for serodiagnosis of lyme borreliosis. | western blot analyses of the human humoral response of patients with lyme borreliosis have shown that a 17-kda protein is an immunodominant protein in late disease. immune electron microscopy with a monoclonal antibody against this protein revealed that the 17-kda protein is abundantly expressed on the surface of borrelia afzelii strain pko. therefore, the protein has been renamed outer surface protein (osp) 17. recombinant osp 17 of strain pko was expressed in escherichia coli and purified by c ... | 1999 | 10363678 |
| validity of interpretation criteria for standardized western blots (immunoblots) for serodiagnosis of lyme borreliosis based on sera collected throughout europe. | western blotting (wb; immunoblotting) is a widely used tool for the serodiagnosis of lyme borreliosis (lb), but so far, no generally accepted criteria for performance and interpretation have been established in europe. the current study was preceeded by a detailed analysis of wb with whole-cell lysates of three species of borrelia burgdorferi sensu lato (u. hauser, g. lehnert, r. lobentanzer, and b. wilske, j. clin. microbiol. 35:1433-1444, 1997). in that study, interpretation criteria for a pos ... | 1999 | 10364592 |
| comparative analysis and immunological characterization of the borrelia bdr protein family. | multiple circular and linear plasmids of lyme disease and relapsing fever borrelia spirochetes carry genes for members of the bdr (borrelia direct repeat) protein family. to define their common and divergent attributes, we first comprehensively compared the known homologs. bdr proteins with predicted sizes ranging from 10.7 to 30. 6 kda formed five homology groups, based on variable numbers of short direct repeats in a central domain and diverse n- and c-terminal domains. in a further characteri ... | 1999 | 10377099 |
| salivary gland extract from ixodes ricinus ticks inhibits production of interferon-gamma by the upregulation of interleukin-10. | tick saliva has been shown to modulate host immunity by a so far unknown mechanism. we have demonstrated an inverted effect of salivary gland extract (sge), derived from partially fed ixodes ricinus females, on the production of two cytokines, interferon (ifn)-gamma and interleukin (il)-10, in vitro. while sge markedly suppressed the elaboration of ifn-gamma by mouse splenocytes stimulated with lipopolysaccharide (lps), the production of il-10 was increased in comparison with sge-untreated cultu ... | 1999 | 10417669 |
| phenotypic and genetic characterization of a novel borrelia burgdorferi sensu lato isolate from a patient with lyme borreliosis. | borrelia burgdorferi sensu lato a14s was cultured from a skin biopsy specimen of a patient with erythema migrans in the netherlands. this isolate had a unique dna fingerprint pattern compared to 135 other b. burgdorferi sensu lato isolates. in this study, the isolate a14s was further characterized by protein analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and reactivity with various monoclonal antibodies. in addition, the 16s rrna, ospa, and ospc genes, as well ... | 1999 | 10449497 |
| persistence of borrelia garinii and borrelia afzelii in patients with lyme arthritis. | we repeatedly detected dna of borrelia garinii or b. afzelii and borrelia-like structures in the blood, joint fluid or in the synovium of 10 patients with lyme arthritis by means of the polymerase chain reaction and immunoelectron microscopy at 2-4-month intervals in the course of two years. all samples were analyzed using primers which amplified the 16s rrna gene sequence of borrelia burgdorferi sensu lato and nucleotide sequences for the ospa gene. no cross hybridization occurred with dna from ... | 1999 | 10467661 |
| evidence for frequent ospc gene transfer between borrelia valaisiana sp. nov. and other lyme disease spirochetes. | molecular polymorphism of the ospc gene has been reported in borrelia burgdorferi sensu stricto, borrelia garinii and borrelia afzelii, the spirochetes causing human lyme borreliosis. to assess the genetic relationship between ospc genes from the recently described borrelia valaisiana sp. nov. and other b. burgdorferi sensu lato species, the ospc genes from eight b. valaisiana isolates were amplified by pcr, cloned and sequenced. the ospc genes of three b. valaisiana isolates were identical, but ... | 1999 | 10474195 |
| incidence of borrelia garinii and borrelia afzelii in ixodes ricinus ticks in an urban environment, prague, czech republic, between 1995 and 1998. | the presence of borrelia burgdorferi sensu lato and its individual genospecies was determined using a single-step polymerase chain reaction in a total of 779 ixodes ricinus ticks collected from an urban park in prague, czech republic. the incidence of borrelia burgdorferi sensu lato was 9.2% in 1995, 3.4% in 1996, 4.5% in 1997 and 2.8% in 1998. the borrelia garinii to borrelia afzelii ratio was 1.4:1 and it did not differ significantly throughout the study period. borrelia burgdorferi sensu stri ... | 1999 | 10482032 |
| molecular typing of borrelia burgdorferi sensu lato: taxonomic, epidemiological, and clinical implications. | borrelia burgdorferi sensu lato, the spirochete that causes human lyme borreliosis (lb), is a genetically and phenotypically divergent species. in the past several years, various molecular approaches have been developed and used to determine the phenotypic and genetic heterogeneity within the lb-related spirochetes and their potential association with distinct clinical syndromes. these methods include serotyping, multilocus enzyme electrophoresis, dna-dna reassociation analysis, rrna gene restri ... | 1999 | 10515907 |
| determination of members of a borrelia afzelii-related group isolated from ixodes nipponensis in korea as borrelia valaisiana. | the 16s rrna sequences of the korean borrelia strains 5mt and 9mt, isolated from ixodes nipponensis, showed identities of 99.0-99.1% to that of b. afzelii. the strains were tentatively classified as belonging to the b. afzelii-related group. in this study, korean isolates, including these strains, were characterized further and compared with recently described new species. these strains generated a rflp pattern that has not been found previously in rflp analysis of the 5s-23s rrna intergenic spa ... | 1999 | 10555320 |
| positive serology for lyme disease borrelias in primary cutaneous b-cell lymphoma: a study in 22 patients; is it a fortuitous finding? | the historical association of acrodermatitis chronica atrophicans (aca), now known to be a late manifestation of lyme disease caused by borrelia afzelii, with cutaneous lymphoma, and several small series of pcbcl with positive lyme disease borrelial serology initiated a study of this association. material and methods in the last 9 years, 30 patients with pcbcl have been observed and followed, 22 of them were tested for borrelial serology. the control group consisted of 85 patients with nhl (10 c ... | 1999 | 10641031 |
| transcriptional analysis of a superoxide dismutase gene of borrelia burgdorferi. | a single superoxide dismutase (sod) gene was identified in borrelia burgdorferi strains, borrelia afzelii ple and borrelia garinii pbi. recombinant enzymatic activity was detected only when sod expression was controlled by the lacz promoter in the cloning vector. northern blot analysis with sod- or seca-specific probes identified a common 3.7-kb transcript. reverse transcriptase-pcr analysis confirmed that seca and sod constitute a single transcriptional unit in b. burgdorferi. a transcriptional ... | 2000 | 10650199 |
| [single-step polymerase chain reaction in the detection of borrelia burgdorferi and the genome species in the ixodes ricinus tick]. | the objective of the work to introduce screening pcr into the diagnosis of borrelia burgdorferi sensu lato in the vector selection of the most suitable primer, derived from chromosomal dna and detection of different genome species. the sensitivity of primers, described in the literature (ld, 16s, wk, 5s-23s) was tested by different amounts of dna strains of borrelias. the most sensitive primer--ld was used for detection of borrelias in the vector. ticks were collected in municipal parks from 199 ... | 1999 | 10658345 |
| identification of borrelia burgdorferi sensu stricto, borrelia garinii and borrelia afzelii in ixodes ricinus ticks from southern bohemia using monoclonal antibodies. | the prevalence of borrelia burgdorferi sensu lato (s.l.) in 761 ixodes ricinus ticks collected in different localities of southern bohemia was investigated. the minimum infection rate of nymphs, males and females as determined by dark-field microscopy was 4.8% in 1997. the total b. burgdorferi prevalence determined in ticks in 1998 was 11.04%. a significant local variability of the prevalence of b. burgdorferi was not found. nine isolates were identified at a genospecies level by immunoblotting ... | 2000 | 10705611 |
| new colorimetric microdilution method for in vitro susceptibility testing of borrelia burgdorferi against antimicrobial substances. | a newly developed colorimetric microdilution method was used to analyze the activity of 12 antimicrobial agents against nine borrelia burgdorferi isolates, including all three genospecies pathogenic for humans. in addition, in vitro antimicrobial resistance patterns of borrelia valaisiana and borrelia bissettii tick isolates were investigated. the applied test system is based upon color changes that occur in the presence of phenol red and result from the accumulation of nonvolatile acid produced ... | 2000 | 10706176 |
| characterization of borrelia burgdorferi strains isolated from korea by 16s rdna sequence analysis and pcr-rflp analysis of rrf (5s)-rrl (23s) intergenic spacer amplicons. | haenam strains of borrelia burgdorferi, which had been isolated from ixodes granulatus and apodemus agrarius in haenam, korea, were characterized by pcr-rflp analysis of rrf (5s)-rrl (23s) intergenic spacer amplicons and by sequence analysis of the 16s rrna gene (rdna). the msel and dral restriction patterns of the 5s-23s intergenic spacer amplicons of haenam strains differed from those of other b. burgdorferi sensu lato strains. furthermore, in the phylogenetic tree based on the 16s rdna sequen ... | 2000 | 10758897 |
| distribution of twelve linear extrachromosomal dnas in natural isolates of lyme disease spirochetes. | we have analyzed a panel of independent north american isolates of the lyme disease agent spirochete, borrelia burgdorferi (sensu stricto), for the presence of linear plasmids with sequence similarities to the 12 linear plasmids present in the b. burgdorferi type strain, isolate b31. the frequency of similarities to probes from each of the 12 b31 plasmids varied from 13 to 100% in the strain panel examined, and these similarities usually reside on plasmids similar in size to the cognate b31 plas ... | 2000 | 10762248 |
| an improved recombinant igg immunoblot for serodiagnosis of lyme borreliosis. | we have previously described the use of the following recombinant antigens for serodiagnostic immunoblots: p83/100, p39, ospc and p41 (flagellin) internal fragment [wilske et al. (1993) med microbiol immunol 182:255-270; rossler et al. (1997) j clin microbiol 35:2752-2758]. in our currently used immunoblot p83/100 is derived from strain pko (borrelia afzelii), p39 (bmpa) and ospc from strains pka2 (b. hurgdorferi sensu stricto), pko and pbi (b. garinii), respectively; the p41 (flagellin) interna ... | 1999 | 10776844 |
| detection and genotyping of borrelia burgdorferi sensu lato by polymerase chain reaction. | to isolate and genotype borrelia burgdorferi genospecies in serum samples of croatian patients with erythema migrans. | 2000 | 10810167 |
| occurrence of borrelia burgdorferi sensu stricto, borrelia garinii and borrelia afzelii in the ixodes ricinus ticks from eastern slovakia. | a total of 2816 unfed adults nymphs of ixodes ricinus ticks were collected from vegetation in kosice (eastern slovakia) from 1994 to 1997. prevalence of borrelia burgdorferi s. l. in i. ricinus ticks, detected by dark field microscopy, varied and depended upon the year and the habitat of the collected ticks. the lowest prevalence was observed in 1994 (4.8%). during 1995 it increased to 17.2% and during the next two years decreased to 15.5% and 14.2%. the rate of infection varied from 2.1 to 23.3 ... | 2000 | 10845258 |
| colorimetric in vitro susceptibility testing of penicillins, cephalosporins, macrolides, streptogramins, tetracyclines, and aminoglycosides against borrelia burgdorferi isolates. | the spectrum of antibiotic susceptibility of borrelia burgdorferi has been only partially defined. in the present study the effectiveness of 12 antimicrobials, belonging to six different antibiotic classes have been tested against borrelia burgdorferi s.s. (n=3), borrelia garinii (n=3), borrelia afzelii (n=3), borrelia valaisiana (n=1), and borrelia bissettii (n=1) isolates. these isolates were analysed by a new standardised colorimetric minimal inhibitory concentration (mic) method based upon c ... | 2000 | 10856671 |
| the cell-mediated immune response to borrelia afzelii, garinii and burgdorferi in c57bl/6 mice is dependent on antigen specificity. | inbred c57bl/6 mice were challenged with borrelia afzelii, borrelia garinii and borrelia burgdorferi sensu stricto and tested for antigen-specific t-cell response in vitro. the sonicated preparations of in vitro grown spirochetes were capable of stimulating polyclonal proliferation and specific cell-mediated response, depending on duration of the cell culture. murine splenocytes previously sensitized to b. burgdorferi sensu lato (s.l. ), but not those from control mice, could be induced for anti ... | 2000 | 10865176 |
| antibodies against specific proteins of and immobilizing activity against three strains of borrelia burgdorferi sensu lato can be found in symptomatic but not in infected asymptomatic dogs. | in an area where lyme disease is endemic in the netherlands all dogs had positive titers by whole-cell enzyme-linked immunosorbent assay and appeared to be naturally infected by borrelia burgdorferi sensu lato. to compare the antibody responses of symptomatic dogs and asymptomatic controls, we performed western blots and in vitro immobilization assays to study antibody-dependent bactericidal activity. strains from three different genospecies were employed as the antigen source: b. burgdorferi st ... | 2000 | 10878052 |
| rapid differentiation of borrelia garinii from borrelia afzelii and borrelia burgdorferi sensu stricto by lightcycler fluorescence melting curve analysis of a pcr product of the reca gene. | to differentiate the borrelia burgdorferi sensu lato genospecies, lightcycler real-time pcr was used for the fluorescence (sybr green i) melting curve analysis of borrelial reca gene pcr products. the specific melting temperature analyzed is a function of the gc/at ratio, length, and nucleotide sequence of the amplified product. a total of 32 dna samples were tested. of them three were isolated from b. burgdorferi reference strains and 16 were isolated from b. burgdorferi strains cultured from i ... | 2000 | 10878080 |
| isolation of borrelia afzelii from overwintering culex pipiens biotype molestus mosquitoes. | during the years 1995-1996, a total of 1,743 overwintering culex pipiens biotype molestus female mosquitoes were tested for the presence of spirochetes in several localities in south moravia, czech republic.the spirochetes were observed in 5% of the mosquitoes investigated. one of the five isolated strains of spirochetes (br-84) was identified as borrelia afzelii. the potential role of mosquitoes in the ecology and epidemiology of lyme disease (ld) borreliae should be further investigated. | 1999 | 10885843 |
| diagnosis of lyme disease in two cows by the detection of borrelia burgdorferi dna. | two cows from different herds in a district of switzerland known to harbour ixodid ticks had erythematous lesions on the hairless skin of the udder, were in poor general condition with a poor appetite and decreased milk production, and had a stiff gait and swollen joints. borrelia burgdorferi sensu strictu dna was detected in samples of synovial fluid and milk from one of the cows and borrelia afzelii dna was detected in synovial fluid from the other by means of a real-time pcr. | 2000 | 10887997 |
| lyme disease. | lyme disease (ld) is the most common tick-borne disease in the us. the overall trend has been an average annual increase in cases since surveillance was initiated by the centers for disease control and prevention in 1982. to date, 10 different borrelia species have been described within the borrelia burgdorferi sensu lato complex, although only borrelia burgdorferi sensu strico, borrelia garinii, and borrelia afzelii have been associated with human disease. ixodes ticks often carry more than one ... | 2000 | 10910185 |
| borreliacidal activity of early lyme disease sera against complement-resistant borrelia afzelii fem1 wild-type and an ospc-lacking fem1 variant. | sera obtained from 14 lyme borreliosis patients at early stages (stages i and ii) of the disease were examined for their borreliacidal properties against borrelia afzelii isolate fem1 by use of a growth inhibition assay. five of 14 immune sera exhibited borreliacidal activity against isolate fem1. heat-inactivated immune sera failed to kill the spirochaetes. immunoblotting experiments with outer-membrane preparations showed that ospc and 11 additional proteins of 14.0, 16.0, 17.7, 19.3, 21.7, 27 ... | 2000 | 11023189 |
| relative incompetence of european rabbits for lyme disease spirochaetes. | to determine whether rabbits may serve as reservoir hosts for lyme disease spirochaetes in europe, we compared their competence as hosts for borrelia afzelii, one of the most prevalent european spirochaetal variants, with that of the mongolian jird. to infect rabbits or jirds, at least 3 nymphal or adult ixodes ricinus ticks infected with spirochaetes fed to repletion on each animal. whereas jirds readily acquired tick-borne lyme disease spirochaetes and subsequently infected vector ticks, rabbi ... | 2000 | 11085249 |
| the complement regulator factor h binds to the surface protein ospe of borrelia burgdorferi. | spirochete bacteria of the borrelia burgdorferi sensu lato complex cause lyme borreliosis. the three pathogenic subspecies borrelia garinii, borrelia afzelii, and borrelia burgdorferi sensu stricto differ in their disease profiles and susceptibility to complement lysis. we investigated whether complement resistance of borreliae could be due to acquisition of the main soluble inhibitors of the alternative complement pathway, factor h and the factor h-like protein 1. when exposed to nonimmune edta ... | 2001 | 11113124 |
| tick salivary gland extract inhibits killing of borrelia afzelii spirochetes by mouse macrophages. | salivary gland extract (sge) from ixodes ricinus ticks inhibited the killing of borrelia afzelii spirochetes by murine macrophages. sge also reduced the production of two major defense molecules of phagocytes, superoxide and nitric oxide. it is likely that the suppression of macrophage microbicidal mechanisms contributes to the inhibitory effect of tick saliva on the killing of b. afzelii spirochetes, thus facilitating the transmission of this important pathogen. | 2001 | 11119556 |
| treatment resistant lyme arthritis caused by borrelia garinii. | lyme arthritis is caused in europe by three main pathogenic species of borrelia burgdorferi sensu lato: borrelia burgdorferi sensu stricto, borrelia garinii, and borrelia afzelii. because few synovial samples have yet been analysed by species-specific dna amplification methods, further studies are needed to define the spectra of clinical manifestations associated with these different species. two cases of treatment resistant lyme arthritis are reported here, in which dna amplification of the fla ... | 2001 | 11171694 |
| rapid typing of borrelia burgdorferi sensu lato species in specimens from patients with different manifestations of lyme borreliosis. | to further investigate the pathogenic potential of different borrelia burgdorferi genospecies, specimens from 27 patients with different manifestations of lyme borreliosis were analyzed by pcr and reverse line blotting (rlb). in samples from lyme arthritis patients, b. burgdorferi sensu stricto was predominantly identified, while in patients with neuroborreliosis or acrodermatitis, borrelia garinii and borrelia afzelii, respectively, were exclusively detected. the results demonstrate that pcr-rl ... | 2001 | 11230440 |
| isolation and polymerase chain reaction typing of borrelia afzelii from a skin lesion in a seronegative patient with generalized ulcerating bullous lichen sclerosus et atrophicus. | a 64-year-old woman presented with bullous and ulcerating lichen sclerosus et atrophicus (lsa) on the neck, trunk, genital and perigenital area and the extremities. histology of lesional skin showed the typical manifestations of lsa; in one of the biopsies spirochaetes were detected by silver staining. despite treatment with four courses of ceftriaxone with or without methylprednisone for up to 20 days, progression of lsa was only stopped for a maximum of 1 year. spirochaetes were isolated from ... | 2001 | 11251580 |
| characterization of lyme borreliosis isolates from patients with erythema migrans and neuroborreliosis in southern sweden. | southern sweden is an area of lyme borreliosis (lb) endemicity, with an incidence of 69 cases per 100,000 inhabitants. the most frequent clinical manifestations are erythema migrans (77%) and neuroborreliosis (16%). there was no record of human borrelia strains being isolated from patients in this region before the prospective study reported here. borrelia spirochetes were isolated from skin and cerebrospinal fluid (csf) from lb patients living in the region. a total of 39 strains were character ... | 2001 | 11283044 |
| cell-mediated immune response to high-passage borrelia spirochetes in c57bl/6 mice is strictly dependent on antigen specificity. | inbred c57bl/6 mice were challenged with high-passage borrelia afzelii, borrelia garinii and borrelia burgdorferi sensu stricto and tested for antigen specific t-cell response in vitro. sonicated preparations of washed spirochetes were potent cell activators, capable of stimulating polyclonal proliferation after 72h of culture while increasing the incubation time up to 120h provoked specific cell-mediated response. isolated murine spleocytes previously sensitized to b. burgdorferi sensu lato but ... | 2001 | 11297365 |
| distinct levels of genetic diversity of borrelia burgdorferi are associated with different aspects of pathogenicity. | different species of pathogenic borrelia show different symptoms and tick vector specificity. even within regions where only one species is found, lyme disease progresses very differently from one patient to another. since borrelia shows very little recombination either within or between species, alleles of a gene can be used to mark clones. the ospc gene is highly variable within each species and can be used to define groups of related clones. it has been previously shown that only four out of ... | 2001 | 11316368 |
| [culture of borrelia burgdorferi sensu lato from patients in the czech republic]. | spirochete of borrelia burgdorferi sensu lato were isolated in a modified bsk medium from 26 patients of 275 investigated suffering from early and late borreliosis. the isolates were specified by phenotype and genotype analysis using monoclonal antibodies, by immunochemical methods and by including species specific 16s rrna, ospa and ospc primers and probes in a polymerase chain reaction with hybridization. borrelia afzelii was found in 9 patients, borrelia garinii in 16 and b. burgdorferi sensu ... | 2001 | 11329731 |
| isolation of borrelia burgdorferi sensu lato from blood of patients with erythema migrans. | we assessed the isolation rate of borrelia burgdorferi sensu lato from blood in european patients with typical erythema migrans and evaluated the course and outcome of their illness. | 2001 | 11339477 |
| [antibodies against borrelia afzelli in patients with an early stage of lyme disease]. | the aim of the study was evaluation of specific immune response against borrelia afzelii in patients with erythema migrans, as a sign of an early stage of lyme borreliosis. the study was performed in 42 patients, residents of podlasie province, who were initially evaluated by indirect immunofluorescence assay as a seropositive for borrelia burgdorferi sensu lato. antibodies against particular antigens of b. afzelii were demonstrated using a western-blot technique. evaluation of results showed th ... | 2001 | 11344697 |
| [first detection of ehrlichiosis detected serologically and with the polymerase chain reaction in patients with borreliosis in the czech republic]. | infectious complications caused by genus ehrlichia were confirmed when samples taken from patients with lyme borreliosis with non-characteristic development and those sent directly by physician who treated the patient were examined. laboratory diagnostics of ehrlichiosis was based on hematological examination of blood, on anamnestic and epidemiological data, on indirect immunofluorescence serology verified using western blot test with specific antigen and by determination of dna agents using pol ... | 2001 | 11347209 |
| complement evasion by borrelia burgdorferi: serum-resistant strains promote c3b inactivation. | the most characteristic features of the lyme disease pathogens, the borrelia burgdorferi sensu lato (s.l.) group, are their ability to invade tissues and to circumvent the immune defenses of the host for extended periods of time, despite elevated levels of borrelia-specific antibodies in serum and other body fluids. our aim in the present study was to determine whether b. burgdorferi is able to interfere with complement (c) at the level of c3 by accelerating c3b inactivation and thus to inhibit ... | 2001 | 11349031 |
| western blot analysis for diagnosis of lyme disease in acute facial palsy. | lyme borreliosis has been implicated in the pathogenesis of acute peripheral facial palsy (apfp). few studies, however, have used western blot analyses to confirm the serological diagnosis. | 2001 | 11359146 |
| identification of ehrlichia spp. and borrelia burgdorferi in ixodes ticks in the baltic regions of russia. | the presence and distribution of ehrlichia spp. and borrelia burgdorferi sensu lato was demonstrated among ixodid ticks collected in the baltic regions of russia, where lyme borreliosis is endemic. a total of 3,426 ixodes ricinus and 1,267 ixodes persulcatus specimens were collected, and dark-field microscopy showed that 265 (11.5%) i. ricinus and 333 (26.3%) i. persulcatus ticks were positive. from these samples, 472 dark-field-positive and 159 dark-field-negative ticks were subjected to pcr an ... | 2001 | 11376063 |
| isolation and characterization of borrelia burgdorferi sensu lato strains in an area of italy where lyme borreliosis is endemic. | between 1993 and 1998, we isolated borrelia burgdorferi sensu lato from 55 of the 119 patients with clinically diagnosed lyme borreliosis who were admitted to "san martino" hospital in belluno, veneto, an adriatic region in northeastern italy where lyme borreliosis is endemic. upon hospitalization, all patients presented erythema migrans. isolates were typed using ribosomal dna pcr-restriction fragment length polymorphism (rflp) analysis of the rrfa-rrlb intergenic spacer. of the 41 isolates typ ... | 2001 | 11376066 |
| inhibition of borrelia burgdorferi-tick interactions in vivo by outer surface protein a antibody. | borrelia burgdorferi outer surface protein (osp) a is preferentially expressed by spirochetes in the ixodes scapularis gut and facilitates pathogen-vector adherence in vitro. here we examined b. burgdorferi-tick interactions in vivo by using abs directed against ospa from each of the three major b. burgdorferi sensu lato genospecies: b. burgdorferi sensu stricto, borrelia afzelii, and borrelia garinii. abs directed against b. burgdorferi sensu stricto (isolate n40) destroy the spirochete and can ... | 2001 | 11390491 |
| visualization of borrelia burgdorferi sensu lato by fluorescence in situ hybridization (fish) on whole-body sections of ixodes ricinus ticks and gerbil skin biopsies. | the objective of this study was to visualize borreliae directly in whole-body sections of ixodes ricinus by fluorescence in situ hybridization (fish). borrelia afzelii mono-infected or borrelia burgdorferi sensu stricto (ss)/b. afzelii double-infected nymphs were fixed, embedded in cold polymerizing resin and sectioned. the same sample processing was applied to skin biopsies taken from a mongolian gerbil after an infectious tick-bite. fish was carried out using 16s-rrna-directed, fluorescence-la ... | 2001 | 11390674 |
| sensitive detection of borrelia burgdorferi sensu lato dna and differentiation of borrelia species by lightcycler pcr. | in order to differentiate species within the borrelia burgdorferi sensu lato complex, lightcyler pcr and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the reca gene, were performed. it was demonstrated that nested lightcycler pcr amplification of p66 is more sensitive in the detection of borrelia dna than amplification of the reca gene. b. burgdorferi sensu stricto could be differentiated from borrelia garinii and borrelia afzelii by melting ... | 2001 | 11427590 |
| evidence of ehrlichiosis agents found in ticks (acari: ixodidae) collected from migratory birds. | two ehrlichia pathogens were found in immature ixodes ricinus (l.) ticks collected from migratory passerine birds in the curonian spit area of the baltic region of russia (kaliningrad enclave). during the spring and fall of 2000, 1,606 passerine birds (eight species) were collected; 6.8% of them (110/1,606) were infested by ticks, and 51.8% (57/110) of tick clusters contained various human pathogenic microorganisms. human monocytic ehrlichiosis (hme) and human granulocytic ehrlichiosis (hge) age ... | 2001 | 11476325 |
| isolation of borrelia afzelii from a dog. | 2001 | 11486768 | |
| borrelia burgdorferi sensu lato and ehrlichia spp. in ixodes ticks from southern norway. | we report the results of a study of the prevalence of ehrlichia and borrelia species in 341 questing ixodes ricinus ticks from two locations in southern norway. the prevalences of borrelia burgdorferi sensu lato and ehrlichia spp. were, respectively, 16 and 11.5% at site 1 and 17 and 6% at site 2. prevalence and species composition of borrelia and ehrlichia varied with location and date of collection. the dominant borrelia species at both sites was borrelia afzelii, followed by borrelia burgdorf ... | 2001 | 11574588 |
| routine diagnosis of borrelia burgdorferi (sensu lato) infections using a real-time pcr assay. | to establish a one-tube fluorogenic real-time pcr assay for routine detection of borrelia burgdorferi (sensu lato) dna in various clinical specimens. | 2001 | 11678928 |
| lyme disease borrelia spp. in ticks and rodents from northwestern china. | in may 1999, field surveys of lyme disease spirochetes were conducted around the tianshan mountains in xinjiang uygur autonomous region in northwestern people's republic of china. ixodes persulcatus ticks were obtained in a tianchi lake valley with primary forest, while the tick fauna was poor in the semidesert or at higher altitudes in this region. species identities were confirmed by molecular analysis in which an internal transcribed spacer sequence was used. of 55 adult ticks, 22 (40%) were ... | 2001 | 11679340 |
| [use of indirect micromodification of immunoenzyme technique for serologic detection of ixodes tick-borne borreliosis (comparative analysis)]. | three hundred and seventy one sera sampled in different period since the onset of the disease from 214 patients with ixodes tick-borne borreliosdes (itbb) caused by borrelia afzelii and b. garinii in the perm region (russia) and 299 sera from 222 patients with other diseases were simultaneously tested for igm and igg antibodies by using immunofluorescence assay (ifa) with standard antigen from strain ip-21 b. afzelli by indirect micromodification of enzyme immunoassay (imelisa) on slides and rou ... | 2001 | 11702452 |
| further characterization of complement regulator-acquiring surface proteins of borrelia burgdorferi. | the three genospecies borrelia burgdorferi, borrelia garinii, and borrelia afzelii, all causative agents of lyme disease, differ in their susceptibilities to human complement-mediated lysis. we recently reported that serum resistance of borrelias correlates largely with their ability to bind the human complement regulators fhl-1/reconectin and factor h. to date, two complement regulator-acquiring-proteins (crasp-1 and crasp-2) have been identified in serum-resistant b. afzelii isolates (p. kraic ... | 2001 | 11705962 |
| [criteria for evaluation of immunoblots using borrelia afzelii, borrelia garinii and borrelia burgdorferi sensu stricto for diagnosis of lyme borreliosis]. | the immunoblot was prepared from genotypes borrelia afzelii (kc 90), borrelia garinii (m 192) and borrelia burgdorferi sensu stricto (b 31). sera of 63 patients with different forms of lyme borreliosis were examined and 40 healthy donors in the endemic area of the disease. in class igm in the group of patients significantly more frequently antibodies against ospc, p39, p41 b. afzelii, p39, p41, p66, p83 b. garinii and ospc1, ospa, b. burgdorferi sensu stricto were found. in class igg there were ... | 2001 | 11769176 |
| distribution of clinically relevant borrelia genospecies in ticks assessed by a novel, single-run, real-time pcr. | a lightcycler-based pcr protocol was developed which targets the ospa gene for the identification and quantification of the different borrelia burgdorferi sensu lato species in culture and in ticks, based on the use of a fluorescently labeled probe (hybprobe) and an internally labeled primer. the detection limit of the pcr was 1 to 10 spirochetes. a melting temperature determined from the melting curve of the amplified product immediately after thermal cycling allowed the differentiation of the ... | 2002 | 11773090 |
| experimental infection of laboratory mice and rabbits with several isolates of borrelia burgdorferi sensu lato; comparison of antigens from different genospecies in serological measurement of immune responses. | infectivity of and immune responses to 28 finnish borrelia burgdorferi sensu lato isolates was studied in 3-4-week-old outbred nmri and inbred balb/c/hy laboratory mice; rabbits were also inoculated. twenty-one isolates were found to detectably infect mice. a variation among isolates in degree of infectivity was observed. higher infection rate and higher average elisa readings were recorded for intradermal than intraperitoneal inoculations. the results suggest differences between borrelia genosp ... | 2002 | 11848128 |
| detection of borrelia afzelii, borrelia burgdorferi sensu stricto, borrelia garinii and group vs116 by pcr in skin biopsies of patients with erythema migrans and acrodermatitis chronica atrophicans. | objective: to evaluate the diagnostic performance of two polymerase chain reaction (pcr) procedures using skin biopsies of 20 erythema migrans (em) and 24 acrodermatitis chronica atrophicans (aca) patients. methods: one assay amplified a fragment of the outer surface protein (osp) a gene. the second method amplified the spacer region between the 5s and 23s rrna genes; hybridization of this fragment allowed identification of borrelia burgdorferi sensu lato species. results: among em patients, bot ... | 1997 | 11864084 |
| acrodermatitis chronica atrophicans and serologic confirmation of infection due to borrelia afzelii and/or borrelia garinii by immunoblot. | 1998 | 11864312 | |
| lyme disease and current aspects of immunization. | lyme disease is a tick-borne multisystem disease that affects primarily the skin, nervous system, heart and joints. at least three species of borrelia burgdorferi sensu lato, namely borrelia burgdorferi sensu stricto, borrelia garinii, and borrelia afzelii, can cause the disease. this review will focus mainly on the pathophysiology of lyme arthritis, the long-term outcome of lyme disease, and the recently licensed vaccine against lyme disease. | 2002 | 11879534 |
| dynamics of dissemination and outer surface protein expression of different european borrelia burgdorferi sensu lato strains in artificially infected ixodes ricinus nymphs. | unfed ixodes ricinus nymphs were infected with eight different strains and clones of borrelia afzelii and b. garinii by capillary feeding. except one b. afzelii clone, all expressed ospc in culture. tick midguts and salivary glands were investigated at different time intervals for the presence of borreliae and for ospa and ospc phenotypes by immunofluorescence with simultaneous staining of ospa and ospc with monoclonal antibodies. both species were transmittable to i. ricinus. all ospc-expressin ... | 2002 | 11923373 |
| [borrelia strains that cause erythema migrans in alsace, france]. | in europe, borrelia burgdorferi sensu stricto, borrelia afzelii and borrelia garinii are known as pathogens among the borrelia burgdorferi sensu lato group. since it is not yet known which borrelia are responsible for lyme borreliosis in france, the objective of this study was to identify the species of borrelia responsible for erythema migrans in the region of alsace, france. | 2002 | 11937923 |
| [a patient with neuroborreliosis presenting gadolinium-enhanced mri lesions in bilateral facial nerves]. | we report a 33-year-old man with bilateral facial paralysis due to neuroborreliosis. about three weeks after rhinorrhea and fever lasting four days, he noticed fatigue in the legs and paresthesia in all four extremities. another week later, he developed paresthesia in his tongue and bilateral facial muscle weakness, and was admitted to our hospital. on admission, neurological examination revealed moderate bilateral facial muscle weakness, mild paresthesia in the tongue and four extremities, and ... | 2001 | 11968752 |
| [lyme borreliosis--incidence of serum anti-myelin antibodies]. | the method of enzyme immunoassay (elisa) was used for detection of antibodies against the basic protein myelin (antimyelin antibodies) for a group of serum samples (n 36) with positive anti-borrelia immunoglobulins igg and igm (elisa-borrelia afzelii) and their immune complexes (elisa-peg). antimyelin antibodies (elisa-doxa kit-myelin basic protein antibodies) were assessed in 31% (n 11) of examined serum samples of patients with the working diagnosis of lyme borreliosis. statistical analysis (p ... | 2002 | 11987581 |
| dna microarray assessment of putative borrelia burgdorferi lipoprotein genes. | a dna microarray containing fragments of 137 borrelia burgdorferi b31 putative lipoprotein genes was used to examine lyme disease spirochetes. dna from b. burgdorferi sensu stricto b31, 297, and n40; borrelia garinii ip90; and borrelia afzelii p/gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. these data show that a dna microarray can globally examine the genes encodin ... | 2002 | 12011030 |
| retrobulbar neuritis associated with borrelia afzelii infection. | to report retrobulbar neuritis caused by borrelia afzelii culturally proved from cerebrospinal fluid (csf). | 2002 | 12061028 |
| three major lyme borrelia genospecies (borrelia burgdorferi sensu stricto, b. afzelii and b. garinii) identified by pcr in cerebrospinal fluid from patients with neuroborreliosis in sweden. | the lyme borrelia genospecies borrelia afzelii and b. garinii have previously been isolated using a culture method in swedish patients with lyme borreliosis (lb). there are reports suggesting that the genospecies distribution in human tissue specimens as determined by molecular methods is different from that obtained by culture. in the present study, we developed a nested pcr for detection of lyme borrelia-specific dna in cerebrospinal fluid from swedish patients with lb. the genospecies were su ... | 2002 | 12069016 |
| epitope mapping of the outer surface protein a (ospa) of the spirochete borrelia burgdorferi using a panel of monoclonal antibodies and lanthanide competition fluoroimmunoassay. | a panel of fourteen different monoclonal antibodies was used for detection and analysis of antigenic determinants located on the outer surface protein a (ospa) of the spirochete borrelia burgdorferi, which is a causative agent of tick-borne borreliosis (lyme disease). two main and several minor partially overlapping antigenic determinants have been found on the surface of the ospa protein of borrelia burgdorferi sensu stricto (strain 297) by lanthanide competition fluoroimmunoassay. one of the m ... | 2002 | 12126471 |
| horses and borrelia: immunoblot patterns with five borrelia burgdorferi sensu lato strains and sera from horses of various stud farms in austria and from the spanish riding school in vienna. | grazing animals are continuously exposed to tick bites. consequently, one may expect that horses will become infected with the various pathogens carried by ticks including borrelia burgdorferi sensu lato. whether horses may develop clinical disease due to this pathogen is controversially discussed. we were interested to learn about the infection of horses with borrelia burgdorferi sensu lato within one season by studying the dynamics of the humoral immune response in paired blood samples. the ma ... | 2002 | 12141766 |
| tick salivary gland extract-activated transmission of borrelia afzelii spirochaetes. | saliva-activated transmission of borrelia afzelii canica, nato, du merle, mazie, baranton et postic, 1993 was demonstrated using salivary gland extract (sge) from ixodes ricinus (l., 1758) ticks and c3h mice. injection of borrelia spirochaetes together with sge increased the level of bacteraemia and accelerated the appearance of bacteria in the urinary bladder, compared with the injection of spirochaetes alone. more i. ricinus nymphs became infected when feeding on mice inoculated with b. afzeli ... | 2002 | 12194488 |
| nodular fasciitis, erythema migrans, and oligoarthritis: manifestations of lyme borreliosis caused by borrelia afzelii. | we describe a 35-year old patient with nodular fasciitis, erythema migrans, and gonarthritis four months after a bite of a borrelia afzelii infected tick. the borrelia afzelii infection was identified by a polymerase chain reaction and direct sequencing of the amplification product. borrelia-specific dna was also detectable in nodular fasciitis tissue. we therefore conclude that borrelia afzelii can be a causative agent of nodular fasciitis and lyme arthritis in a highly endemic region of northe ... | 2002 | 12195636 |
| [use of western-immunoblotting to determine serum igg-antibodies to antigens of different genotypes of borrelia--borrelia burgdorferi sensu stricto, borrelia afzelii, and borrelia garinii]. | the reaction of the sera from 86 patients with lyme borreliosis was evaluated in the immunoblotting using three genotypes of pathogenic borrelia strains. the russian isolates of borrelia afzelii (strain ip3) and b. garinii 20047t (strain ip90) were compared with the usa typical strain b31--b. burgdorferi sensu stricto. the results were assessed by the criteria recommended for the usa and developed for b. burgdorferi sensu stricto. certain differences were shown in the reactions of serum igg with ... | 2002 | 12214528 |
| development and laboratory evaluation of a new recombinant elisa for the serodiagnosis of lyme disease. | the use of recombinant proteins for serologic testing represents a modern approach for the improved laboratory diagnosis of lyme disease (ld). the aim of the present study was to develop and evaluate a new recombinant elisa (re) for the detection of specific igg and igm antibodies against borrelia burgdorferi. | 2002 | 12422605 |
| [psoriasis as koebner phenomenon in erythma migrans]. | the koebner phenomenon, described by koebner in 1876, represents the appearance of isomorphic skin lesions in uninvolved skin in patients suffering from certain skin diseases. it can be induced by different mechanical, chemical, thermal and infectious stimuli. a 48 year-old woman developed an exacerbation of a latent psoriasis as a koebner phenomenon in a migrating erythema caused by borrelia afzelii infection. | 2002 | 12444521 |
| strain typing of borrelia burgdorferi, borrelia afzelii, and borrelia garinii by using multiple-locus variable-number tandem repeat analysis. | human lyme borreliosis (lb) is the most prevalent arthropod-borne infection in temperate climate zones around the world and is caused by borrelia spirochetes. we have identified 10 variable-number tandem repeat (vntr) loci present within the genome of borrelia burgdorferi and subsequently developed a multiple-locus vntr analysis (mlva) typing system for this disease agent. we report here the successful application of mlva for strain discrimination among a group of 41 globally diverse borrelia is ... | 2002 | 12454161 |
| apodemus species mice are reservoir hosts of borrelia garinii ospa serotype 4 in switzerland. | among borrelia burgdorferi sensu lato isolates, seven outer surface protein a (ospa) serotypes have been described: serotypes 1 and 2 correspond to b. burgdorferi sensu stricto and borrelia afzelii, respectively, and serotypes 3 to 7 correspond to borrelia garinii. in europe, serotype 4 has never been isolated from ixodes ricinus ticks until recently, although this serotype has been frequently isolated from cerebrospinal fluid from patients. in europe, b. afzelii and b. burgdorferi sensu stricto ... | 2002 | 12454181 |
| characterization of borrelia afzelii isolated from ixodes nipponensis and apodemus agrarius in chungju, korea, by pcr-rflp analyses of ospc gene and rrf (5s)-rrl (23s) intergenic spacer. | nine borrelia afzelii strains, which had been isolated from two vectors, ixodes nipponensis and apodemus agrarius in chungju, korea, were characterized by pcr-rflp analyses of ospc genes and rrf (5s)-rrl (23s) intergenic spacer. drai restriction patterns of chungju strains were identical to those of b. afzelii vs461. but msei restriction patterns of rrf (5s)-rrl (23s) intergenic spacer genes of kk2, km4, kk5 differed from those of previously reported b. burgdorferi sensu lato strains. nine chung ... | 2002 | 12477246 |
| co-feeding transmission and its contribution to the perpetuation of the lyme disease spirochete borrelia afzelii. | to determine whether direct passage of spirochetes between co-feeding vector ticks contributes to the likelihood that the lyme disease spirochete borrelia afzelii will perpetuate in nature, we compared the effects of time and space on transmission efficiency between simultaneously feeding ticks. the likelihood of co-feeding transmission increases with duration of attachment of the infecting tick. co-feeding transmission becomes less efficient as distance from the infecting tick increases. approx ... | 2002 | 12498658 |
| genetic variability within borrelia burgdorferi sensu lato genospecies established by pcr-single-strand conformation polymorphism analysis of the rrfa-rrlb intergenic spacer in ixodes ricinus ticks from the czech republic. | in europe the borrelia burgdorferi sensu lato complex is represented by five distinct genospecies: borrelia burgdorferi sensu stricto, borrelia afzelii, borrelia garinii, borrelia valaisiana, and borrelia lusitaniae. these taxonomic entities are known to differ in their specific associations with vertebrate hosts and to provoke distinct clinical manifestations in human patients. however, exceptions to these rules have often been observed, indicating that strains belonging to a single genospecies ... | 2003 | 12514035 |
| detection and molecular typing of borrelia burgdorferi sensu lato in ixodes ricinus ticks and in different patient samples from southwest germany. | the prevalence of different genospecies of borrelia burgdorferi sensu lato in infected ticks could be a determinant for the risk of acquiring lyme borreliosis (lb) and its clinical presentation. a total of 7373 ticks and 2761 samples from lb patients from the same area in southwest germany were analyzed by pcr to assess the frequency of the occurrence of lb-associated genospecies. fifteen percent of the tick samples and 19% of the human samples were found positive for the presence of b. burgdorf ... | 2001 | 12530764 |
| borrelia afzelii evidenced by polymerase chain reaction in a biopsy of nipple lymphocytoma. | 2003 | 12588408 | |
| characterization of the vls antigenic variation loci of the lyme disease spirochaetes borrelia garinii ip90 and borrelia afzelii acai. | the vls locus of borrelia burgdorferi b31 consists of 15 silent cassettes and one expression site (vlse), and the presence of the encoding plasmid lp28-1 correlates with high infectivity. recombination between the expression cassette and silent cassettes occurs in vivo, and this process may enable b. burgdorferi to evade the immune response. to determine the characteristics of the vls loci in other borrelia strains, we have cloned and characterized the vls silent cassette loci of borrelia garini ... | 2003 | 12603744 |
| association of borrelia afzelii with rodents in europe. | borrelia burgdorferi sensu lato (s.l.) is maintained in nature by complex zoonotic transmission cycles, involving a large variety of vertebrates as hosts and hard ticks of the genus ixodes as vectors. recent studies suggest that the genospecies of b. burgdorferi s.l. and sometimes their subtypes are propagated by different spectra of hosts, mainly birds and rodents. in order to test the concept of host-association, we analysed the relationships between borrelia genospecies, rodent hosts and i. r ... | 2003 | 12613759 |
| prevalence of borrelia burgdorferi species and identification of borrelia valaisiana in questing ixodes ricinus in the lyon region of france as determined by polymerase chain reaction-restriction fragment length polymorphism. | many cases of lyme borreliosis have been reported over the years in the region of lyon, france. the identification and prevalence of borrelia burgdorferi sensu lato in ixodes ricinus were investigated by polymerase chain reaction (pcr) of the flagellin gene and restriction fragment length polymorphism (rflp) analysis. questing ixodes ricinus larvae, nymphs and adults were collected by the flagging method from deciduous forests in four areas in the lyon region of france between october 1994 and s ... | 2003 | 12649714 |
| investigations on the mode and dynamics of transmission and infectivity of borrelia burgdorferi sensu stricto and borrelia afzelii in ixodes ricinus ticks. | borrelia burgdorferi sensu lato (sl), the agent of lyme disease, is transmitted to the host during the blood meal of ixodes ticks. in most unfed ticks, spirochetes are present in the midgut and migrate during blood feeding to the salivary glands, from which they are transmitted to the host via saliva. in the present study, the efficiency of ixodes ricinus ticks to transmit b. afzelii and b. burgdorferi sensu stricto (ss) and their infectivity for mice were examined in relation to the duration of ... | 2002 | 12656125 |
| prevalence of borreliae in ixodid ticks from a floodplain forest ecosystem. | host-seeking ixodid ticks were sampled in a floodplain forest ecosystem along the lower reaches of the thaya (dyje) river in south moravia (czech republic) and lower austria during the period 1989-2002. the ticks were examined by dark-field microscopy for borreliae (borrelia burgdorferi sensu lato, the agent of lyme borreliosis), and attempts were made to culture the spirochetes in bsk-h medium from preparations containing their high numbers. isolated borreliae were identified by pcr-rflp analys ... | 2003 | 12674689 |
| in vitro susceptibility testing of four antibiotics against borrelia burgdorferi: a comparison of results for the three genospecies borrelia afzelii, borrelia garinii, and borrelia burgdorferi sensu stricto. | mics and minimal bactericidal concentrations (mbcs) were evaluated for the four antibiotics azithromycin, amoxicillin, ceftriaxone, and doxycycline against the three main genospecies of borrelia burgdorferi sensu lato. in mbc testing, statistically significant differences between the genospecies could be found in 7 out of 12 comparative evaluations (p < 0.05). | 2003 | 12682190 |
| characteristics of erythema migrans in borrelia afzelii and borrelia garinii infections. | erythema migrans is the most common manifestation of stage i lyme borreliosis. this study investigated the relation between the subspecies of borrelia burgdorferi involved, the time of development and the appearance of the manifestation. the study comprised 33 cases of erythema migrans yielding b. burgdorferi sensu lato by culture of skin biopsy. the species was determined by polymerase chain reaction analysis of the cultivated spirochaetes. in 22 of the cases, b. afzelii was demonstrated, where ... | 2003 | 12685881 |
| prevalence of granulocytic ehrlichia and borrelia burgdorferi sensu lato in ixodes ricinus ticks collected from southwestern finland and from vormsi island in estonia. | altogether, 343 adult and 111 nymphal ixodes ricinus ticks collected from parks in turku and suburban and rural islands of the turku archipelago, finland, and 100 adult i. ricinus ticks collected from vormsi island, estonia, were included in this study. using the polymerase chain reaction the ticks were examined for 16s rdna of the ehrlichia phagocytophila genogroup and for borrelia burgdorferi sensu lato reca and flagellin genes. none of the finnish ticks was found to be infected with e. phagoc ... | 2003 | 12716393 |
| prevalence of four species of borrelia burgdorferi sensu lato and coinfection with anaplasma phagocytophila in ixodes ricinus ticks in central germany. | a total of 305 ixodes ricinus ticks collected from three areas of thuringia in central germany were investigated for infection with borrelia burgdorferi sensu lato species and anaplasma phagocytophila. overall, 11.1% were infected with borrelia burgdorferi and 2.3% with anaplasma phagocytophila. adult ticks showed a significantly higher rate of infection with both borreliae and anaplasma phagocytophila. borrelia garinii (55.9%) was detected most frequently, followed by borrelia burgdorferi sensu ... | 2003 | 12783276 |
| recombinant or peptide antigens in the serology of lyme arthritis in children. | the performance of elisas with the recombinant antigens decorin-binding protein a (dbpa), dbpb, and bbk32 (from borrelia afzelii, b. garinii, and b. burgdorferi sensu stricto) and vlse peptide antigen invariable region 6 (ir(6)) were evaluated in the serodiagnosis and follow-up of children with lyme arthritis (la). serum samples were obtained from 52 children with clinically typical and serologically confirmed la. in igg elisas, at diagnosis, 50 samples were positive for bbk32, 51 for dbpa, 40 f ... | 2003 | 12792865 |
| prevalence of borrelia burgdorferi sensu lato genomospecies and of the human granulocytic ehrlichiosis (hge) agent in ixodes ricinus ticks collected in the area of monti lepini, italy. | ticks are obligate hematophagous arthropods that are parasites in every class of vertebrates in most regions of the world. they are also considered to be important vectors for the transmission of human infectious diseases. in the present study we used polymer chain reaction (pcr) amplification analysis to determine the prevalence of borrelia burgdorferi and ehrlichia phagocytophila, the agents of, respectively, lyme borreliosis and human granulocytic ehrlichiosis, among ticks inhabiting the area ... | 2003 | 12797900 |