Publications

faecal flora of south indian infants and young children in health and with acute gastroenteritis.	the faecal flora of 29 healthy infants and young children was compared with that of 49 children of similar age and socio-ecomonic status with acute gastroenteritis. in the healthy children the most common organisms in the faeces were bifidobacteria, veillonellae, enterobacteria and enterodocci with anaerobes outnumbering aerobes. most members of the noraml faecal flora were present in the diarrhoeal stools, but anaerobes were signigicantly reduced in number and enterobacteria were significantly ...	1978	660639
chemotypes of veillonella lipopolysaccharides.	lipopolysaccharides (lps) were isolated by phenol-water extraction from 34 strains of veillonella, and examined by paper chromatography and colorimetric methods for the presence of neutral sugars, amino sugars and 2-keto-3-deoxy-octonate (kdo). several preparations were also examined for neutral sugars by gas liquid chromatography. the lps had in common glucosamine, galactosamine, l-glycero-d-manno-heptose glucose and kdo. most lps contained galactose, and a few rhamnose. d-glycero-d-manno-hepto ...	1978	665243
effects of prostaglandin on the antigen- and mitogen-driven responses of peripheral blood lymphocytes from patients with adult and juvenile periodontitis.		1978	699390
bacteriology of human experimental gingivitis: effect of plaque age.	twenty-five subjects with previously excellent hygiene and healthy gingiva developed heavy plaque accumulations and bleeding or nonbleeding gingivitis about certain papilla after 21 days of no oral hygiene. gingival marginal plaque about a single papilla was collected at 0, 1, 2, and 3 weeks of no oral hygiene in each subject. the plaque was dispersed, serially diluted, and plated on mm10 sucrose agar in an oxygen-free atmosphere. from 50 to 100 colonies from a single high-dilution plate were ch ...	1978	711336
ticarcillin disodium in anaerobic infections.	twenty-five patients were treated with ticarcillin disodium, 18 of whom had anaerobic infections that included pleuropulmonary infections (seven), mandibular osteomyelitis (four), perirectal abscess (two), sepsis, primary site unknown (one), liver abscess (one), pelvic abscess (one), decubitus ulcer (one), and synergistic gangrene (one). seven had no anaerobic infections. three had anaerobic septicemia. culture results included anaerobes: peptococci (ten), peptostreptococci (ten), bacteroides fr ...	1978	718311
the microbial ecology of the upper small bowel.		1976	775978
microbial transformation of 2,4,6-trinitrotoluene and other nitroaromatic compounds.	a variety of nitroaromatic compounds, including 2,4,6-trinitrotoluene (tnt), were reduced by hydrogen in the presence of enzyme preparations from veillonella alkalescens. consistent with the proposed reduction pathway, r-no2 h2 leads to r-no h2 leads to r-nhoh h2 leads to r-nh2, 3 mol of h2 was utilized per mol of nitro group. the rates of reduction of 40 mono-, di-, and trinitroaromatic compounds by v. alkalescens extract were determined. the reactivity of the nitro groups depended on other sub ...	1976	779650
effect of n-methyl-n'-nitro-n-nitrosoguanidine on the fecal flora of rats.		1976	787606
comparison of the pyrogenicity, limulus activity mitogenicity and complement reactivity of several bacterial endotoxins and related compounds.	the correlation of mitogenicity, complement activation, pyrogenicity and limulus amebocyte lysate (lal) gelation induced by 17 bacterial endotoxins (lps), modified endotoxins, related compounds, and other b cell mitogens was examined. with the use of these compounds, a significant correlation was found between pyrogenicity and mitogenicity (r = 0.85, p is less than 0.0005), pyrogenicity and lal reactivity (r = 0.84, p is less than 0.0005), and mitogenicity and lal reactivity (r = 0.72, p is less ...	1976	789771
the effect of dietary fiber supplementation in man. ii. alteration in fecal physiology and bacterial flora.	fecal weight and water content and fecal quantitative and qualitative bacterial studies were compared on six volunteer subjects during regular diet periods and when on 5.4 g of diet fiber supplementation. there was a statistical mean stool weight increase for the group from 103 +/- 40 g to 226 +/- 90 g. the water content of the stool remained constant under the diet conditions of this study. the total anaerobic counts increased significantly while the subjects were on the high fiber diet. trend ...	1976	793373
susceptibility of anaerobic bacteria to carbenicillin, cefoxitin, and related drugs.	the agar dilution technique was used for determination of the bacteriostatic activity of carbenicillin, penicillin g, cefazolin, cephaloridine, cefoxitin, and cephalothin agaomst a variety of anaerobic bacteria. carbenicillin showed a high level of activity at a concentration of smaller than or equal to 100 mug/ml; only five of 123 strains of bacteroides fragilis, one strain of bifidobacterium eriksonii, and one strain of clostridium bifermentans were resistant to a concentration of larger than ...	1975	804023
effect of sonic treatment on pure cultures and aggregates of bacteria.	pure cultures of a variety of bacteria were treated with ultrasonic energy using a sonic probe. fractions of organisms killed at different sonic energies were calculated, and streptococcus mutans was 600 times more resistant than fusobacterium nucleatum, the most sensitive organism tested. the effects of sonic treatment on aggregates of bacteria were examined, and the results were interpreted as a model of the events that probably occur during the sonic dispersion of dental plaque.	1976	819454
veillonella parvula septicemia and osteomyelitis.		1977	835930
a semiselective medium for the isolation of veillonella species from the mouth.		1977	845267
distribution of xanthine oxidase and xanthine dehydrogenase specificity types among bacteria.	a diverse collection of xanthine-metabolizing bacteria was examined for xanthine-, 1-methylxanthine-, and 3-methylxanthine-oxidizing activity. both particulate and soluble fractions of extracts from aerobically grown gram-negative bacteria exhibited oxidation of all three substrates; however, when facultative gram-negative bacteria were grown anaerobically, low particulate and 3-methylxanthine activities were detected. gram-positive and obligately anaerobic bacteria showed no particulate activit ...	1977	863854
affinity chromatography of antiserum to a gram negative organism.	an immunoadsorbent method is described for the purification of antibody directed against gram negative bacteria. two anion exchange materials were compared for their ability to immobilize whole cells of veillonella alcalescens, a gram negative oral bacterium. purified antibody preparations were applied to the columns and subsequently eluted with various combinations of desorbing buffers. the quantity of recovered antibody was measured and its activity assayed, using a microagglutination techniqu ...	1977	864265
anaerobic ocular infections.		1977	878140
pelvic abscess associated with repeated recovery of veillonella.		1977	900203
nitrate-reductase electron-transport cofactors in veillonella alcalescens.	studies on the effects of inhibitors of the nitrate-reducing activity of veillonella alcalescens extracts suggest the participation of a naphthoquinone, a b-type cytochrome, and non-heme iron in electron transport to nitrate. a nitrate-reductase-deficient mutant displayed a longer doubling time and a decreased molar growth yield on nitrate media. this mutant was phenotypically restored by the addition of molybdate to the growth medium, giving evidence for the functioning of molybdenum in the nit ...	1977	922606
use of preformed cavities in rabbits for the quantitation of leukocyte chemotaxis caused by bacterial lipopolysaccharides.	wound chambers implanted subcutaneously in rabbits proved suitable for measurements of leukocyte chemotaxis. injection of bacterial lipopolysaccharide (lps) in the chambers the sixth day after implantation was followed by a marked increase of polymorphonuclear leukocytes in the wound chamber fluid, the number of which was dependent on the time after application of lps. up to a certain amount of lps, the concentration of leukocytes in the chamber fluid was dose-dependent. the histopathological ap ...	1976	970134
blastogenic response of human lymphocytes to oral bacterial antigens: characterization of bacterial sonicates.	soluble sonicate supernatant preparations were made from actinomyces viscosus (atcc 19246), a. naeslundii (atcc 12104), two strains of veillonella alcalescens (strain hv-1 and a human oral isolate), streptococcus sanguis (atcc 10556), s. mutans (strain 6715-t2), bacteroides melaninogenicus (strain k110), and leptotrichia buccalis (isolated from human dental plaque). these supernatants were characterized with reference to their chemical and antigenic components and their biological activity deter ...	1976	977126
use of hydroxyapatite-coasted glass beads for preclinical testing of potential antiplaque agents.	methods for rapid preclinical testing of antiplaque agents in vitro using hydroxyapatite (ht)-coated glass beads are described. the assays developed could reliably detect (i) prevention of growth in the culture fluid or on the ht surfaces, (ii) the effect of transient exposure of a bactericidal agent on the viability of cells in a preformed bacterial mat, (iii) reversible adsorption of a bactericidal agent on an ht surface, and (iv) the ability of an agent to inhibit adsorption of streptococcus ...	1976	984818
deconjugation of bile acids by human intestinal bacteria.	the purpose of this report is to present the deconjugation of bile acids by numbers of strains of bacteria in the small intestine and feces. the small intestinal juice was aseptically aspirated by a double lumen tube with a rubber cover on the tip devised by us ("fukushima type 1"). bile acids were analyzed with thin layer chromatography. the results: 1) among aerobic bacteria, species of which all of the strains split conjugated bile acids was enterococcus, and most of the strains split were st ...	1976	992250
bacteriology of the lower respiratory tract as determined by fiber-optic bronchoscopy and transtracheal aspiration.	for assessment of the validity of cultures of tracheobronchial secretions and exudates (tbse) obtained by fiber-optic bronchoscopy, the aerobic and anaerobic flora of expectorated saliva and tbse obtained by fiber-optic bronchoscopy from nine healthy volunteers and eight patients were compared with those obtained by fiber-optic bronchoscopy as well). normal volunteers yielded both aerobic and anaerobic bacteria in amounts usually less than 104.5 colony-forming units (cfu)/ml in tbse obtained by ...	1976	993617
[empyema caused by anaerobic flora: clinical and microbiological study of 4 cases].		1976	996980
[changes of antimicrobial susceptibility of anaerobic bacteria from clinical specimens (author's transl)].	the susceptibility to several commonly used antimicrobial agents was examined on approximately 900 isolates of various anaerobes from clinical specimens between october 1965 to may 1975, and the changes of susceptibility were investigated. antimicrobial agents tested were penicillin g (pc-g), ampicillin (ab-pc), cephaloridine (cer), erythromycin (em), josamycin (jm), lincomycin (lcm), clindamycin (cldm), chloramphenicol (cp) and tetracycline (tc). and thiamphenicol (tp) and doxycycline (dotc) we ...	1975	1042183
antibiotic treatment of oral anaerobic infections.		1975	1056466
induction of cellular hypersensitivity to oral microorganisms in guinea-pigs.		1975	1056763
the faecal flora of children in the united kingdom.	the faecal flora of 55 children (aged 8 days to 8 years) and 16 adults was determined. all the children were artificially fed from birth. the faecal flora of the youngest age group was generally less complex and less predictable than that of adults. some bacterial groups commonly found in adult stools, for example bacilli, lactobacilli and yeasts, were rarely found in the youngest infants. most of the changes towards the adult pattern took place between 4 and 12 months. the faecal flora of child ...	1975	1056968
variations in microbial and biochemical components of four-day plaque during a four-week controlled diet period.	variation in microbial and biochemical components of human four-day plaque was studied in seven subjects who were maintained on a high-sucrose diet during a four-week period. significant changes in populations of lactobacilli dextranase-producing organisms, streptococcus mutans, and s sanguis occurred during this period.	1975	1057555
establishment of defined microbial ecosystems in germ-free rats. ii. di-association of osborne-mendel rats with veillonella alcalescens and several oral microorganisms.		1976	1058741
the predominant cultivable organisms in juvenile periodontitis.	quantitative and qualitative examinations of the predominant cultivable microflora of the advanced tissue destruction (pocket) associated with juvenile periodontitis and of control pockets without loss of attachment were carried out by using anaerobic tube culture technique. the microflora from the control sites consisted primarily of streptococci, gram-negative rods, and gram-positive rods. in contrast, the test pockets had a relatively stable and characteristic microflora, dominated by various ...	1976	1061986
isolation of bacteriophages specific for the genus veillonella.		1976	1065266
effects of sodium trimetaphosphate supplementation of a high sucrose diet on the microbial and biochemical composition of four-day plaque and on urine calcium and phosphorus levels.	daily supplementation of a high sucrose diet with 5 gm tmp resulted in significant alterations in certain human plaque components. veillonella and neisseria population (as percent total viable microorganisms), s sanguis (as percent streptococci), and levan hydrolase activity per milligram of plaque increased, whereas total viable microorganisms per milligram of plaque, streptococci per milligram of plaque, and specific fructosyltransferase activity decreased during the tmp-containing diet regime ...	1976	1067293
cigarette smoking and the microbial flora of the mouth.	a range of selective media was used to culture the microbial flora of the dental plaque, tongue and palate, the subjects were five young men who smoked more than twenty cigarettes a day and four who did not smoke. neisseriae were less numerous on the mucosal surfaces of the smokers.	1976	1068665
studies on veillonellophages isolated from washings of human oral cavity.		1976	1072190
chemically defined media for growing anaerobic bacteria of the genus veillonella.	a chemically defined medium for veillonella parvula and v. alcalescens is described. some nutritional aspects of the two strains used were examined: the optimum concentration of reducing agents, the requirements for amino acids, diamines, vitamins and other growth factors, and the conditions needed for well balanced nutrition. no specific requirements for single amino acids were observed. a combination of l-cysteine, dl-aspartic acid, l-glutamic acid, l-serine and l-tyrosine, promoted growth. in ...	1976	1087858
transient bacteremia associated with barium enema.	a group of 175 patients had barium enema. pour-plate blood cultures were obtained immediately before and after the procedure and 5, 10, 15, and 30 minutes later. bacteremia was demonstrable in 20 (11.4%) patients. in some, blood cultures were positive for as long as 15 minutes after barium enema; all were negative at 30 minutes. among the bacteria associated with the 20 episodes of bacteremia were escherichia coli, klebsiella, enterococci, proteus morganii, bacteroides, and veillonella. the inci ...	1975	1093504
symbiosis of streptococcus mutans and veillonella alcalescens in mixed continuous cultures.		1975	1096856
bacterial studies of clindamycin-associated colitis. a preliminary report.	the cause of pseudomembraneous colitis is not known but has been attributed to an alteration in the microbial flora of the colon. to test this hypothesis, a blind, prospective study was undertaken in which fecal samples were cultured quantitatively for aerobic and anaerobic organisms. the patients from which these samples were taken were all receiving clindamycin and had diarrhea secondary to the use of the drug. we were able to show definite differences in the colonic microflora which correlate ...	1975	1097297
disk susceptibility testing of slow-growing anaerobic bacteria.	the susceptibility of 55 strains of slow-growing anaerobes to eight clinically useful or potentially useful antibiotics was determined by agar dilution and disk diffusion tests. strains of the genera peptococcus, peptostreptococcus, megasphaera, veillonella, eubacterium, bifidobacterium, clostridium, and fusobacterium were included. all strains were susceptible to chloramphenicol, but varied in their susceptibility to penicillin, lincomycin, clindamycin, tetracyclines, and vancomycin. correlatio ...	1975	1137353
accumulation of phosphatidylserine in strictly anaerobic lactate fermenting bacteria.		1975	1140395
evaluation of human oral organisms and pathogenic streptococcus for production of iga protease.	iga protease is a proteolytic enzyme found in whole human saliva and in dental plaque that cleaves both secretory and myeloma iga of human origin to yield intact fabalpha and fcalpha fragments. to determine which bacteria are capable of producing this enzyme, we have examined a variety of strains normally found in the human oral cavity and a number of streptococci of known lancefield group serotype. streptococci of groups a, b, c, d, f, g, h, m, and n, streptococcus mutans, streptococcus sanguis ...	1975	1168676
characterization of a naturally occurring diamine auxotroph of veillonella alcalescens.	veillonella alcalescens strain atcc 17745 was shown to require putrescine or cadaverine for growth. none of the other compounds tried, including magnesium and spermidine, were able to substitute for the diamines. studies with labeled diamines showed that spermidine was made from putrescine in this organism. a polyamine analogous to spermidine, but made from cadaverine, was not found. a combination of growth experiments and chemical assays suggested that protein synthesis was limited in diamine-s ...	1975	1194234
lipid-phase transitions of the strictly anaerobic bacteria veillonella parvula and anaerovibrio lipolytica.	as a basis for physicochemical studies on the membranes of the strictly anaerobic bacteria veillonella parvula, anaerovibrio lipolytica, and megasphaera elsdenii, the fatty acyl and alk-1-enyl moieties on the phosphoglycerides of these organism were characterized. uncommon is the high proportion of a heptadecenoic acyl and alk-1-enyl moiety in these three lactate-fermenting bacteria. in contrast to v. parvula and a. lipolytica, m. elsdenii contains high amounts of branched-chain acyl and alk-1-e ...	1975	1194242
iron-sulphur systems in some isolated multi-component oxidative enzymes.		1975	1237425
cell wall composition and incorporation of radio-labelled compounds by veillonella alcalescens.	the cell wall of veillonella alcalescens was shown to have a typically gram-negative appearance and composition. the wall contains 24% lipid, 0.8% phosphorus, and 6.8% hexosamine. it is estimated to contain about 5% murein, unlike the 24% reported by other for veillonella parvula. the amounts of 19 amino acids, including diaminopimelic acid, were determined. though veillonella sp. cannot metabolize sugars for energy, v. alcalescens incorporates ribose and fructose by separate, specific mechanism ...	1975	1240789
[antibacterial and bactericidal activity of tinidazole against anaerobic bacteria comparing with metronidazole (author's transl)].	antibacterial activity of tinidazole (1-2-(ethylsulfonyl)-2-methyl-5-nitroimidazole) against anaerobic bacteria including peptococcus, peptostreptococcus, eubacterium, propionibacterium, bacteroides and fusobacterium was studied by agar dilution method comparing with metronidazole. in addition to this work, bactericidal effect of tinidazole and metronidazole against p. prevotii, b. fragilis ss. fragilis and f. varium was examined by quantitative culture method after incubation in gam broth conta ...	1976	1271586
resistance of anaerobic bacteria to antimicrobial agents in spain.	as a consequence of antibiotic consumption, the entire microbial ecosystem attached to man is evolving towards resistance. in spain, penicillin resistance (mic > 0.5 mg/l) is found in about 10% of peptostreptococcus, clostridium perfringens and eubacterium, and in 50% of veillonella. cefoxitin resistance ( > 2 mg/l) is present in 10 to 20% of peptostreptococcus and clostridium, and in 50% of eubacterium; 21% of bacteroides (fragilis group) strains are resistant to 16 mg/l. a very low rate of imi ...	1992	1295754
[roxithromycin in dental infections].	roxithromicin is a semisynthetic macrolide antibiotic, with similar antibacterial activity to erythromycin. it is characterized by an excellent pharmacokinetic profile and a good tissue penetration. particularly at the dose of 300 mg per day the dental tissue diffusion is extremely good, and this is important to oppose the oral cavity infections. oral cavity infections can be either odontogenic or non odontogenic. odontogenic infections are typically primary and are caused by commensal bacteria ...	1992	1301492
the use of dna probes to examine the distribution of subgingival species in subjects with different levels of periodontal destruction.	the present investigation examined the distribution of 14 subgingival species at a total of 2299 sites in 90 subjects with different levels of periodontal destruction. subgingival plaque samples taken from the mesial aspect of each tooth were anaerobically dispersed, diluted and plated on non selective media. after anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxygenin-labeled whole chromosomal dna probes. the mean total viable count for all s ...	1992	1318331
subgingival temperature (iii). relation to microbial counts.	the present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. at each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. after anaerobic incuba ...	1992	1321846
effect of piperacillin/tazobactam therapy on intestinal microflora.	the effect of piperacillin/tazobactam treatment upon the intestinal microflora was studied in 20 patients with intraabdominal infections. the patients received piperacillin 4 g combined with tazobactam 500 mg q 8 h by intravenous injection for 4-8 days. stool specimens were collected before, during and after therapy for cultivation of aerobic and anaerobic microorganisms. six patients had measurable concentrations of piperacillin (1.2-276 mg/kg/faeces) and 4 patients tazobactam concentrations (0 ...	1992	1322559
microbiologic and pathologic aspects of pulpal and periapical disease.	the greatest cause of endodontic and periapical pathosis is microbial infection of the pulp. most odontogenic infections are of a polymicrobial nature. with advances in anaerobic isolation and culturing techniques, much has been learned about the presence of pathogenic organisms such as porphyromonas and prevotella species (formerly classified as black-pigmented bacteroides species) in infected root canals. this review provides a summary of recent developments in endodontic microbiology, virulen ...	1992	1381632
bacterial invasion of non-exposed dental pulp.	anaerobic procedures were adopted to demonstrate the early bacterial invasion of non-exposed dental pulps, and to isolate and identify the bacteria. of 19 freshly extracted teeth which originally exhibited deep dentinal lesions, clinical examination and electric pulp testing showed that nine of them had no pulpal exposure. thus the pulps of these teeth were covered by clinically sound dentine beneath the carious lesion. bacteria were found to have invaded the pulps of six of these nine teeth. th ...	1992	1399049
experimentally induced infection by oral anaerobic microorganisms in a mouse model.	a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ...	1992	1408362
anaerobic microflora associated with the pars oesophagea of the pig.	in healthy pigs aged one to 35 days significant populations of microorganisms (5.5 to 6.9 log10 viable count cm-2 of the tissue) were found, by strict anaerobic techniques, to adhere to the pars oesophagea. the genera of anaerobes which were isolated included actinomyces, bifidobacterium, clostridium, lactobacillus, peptostreptococcus, streptococcus and veillonella and facultative anaerobes included escherichia, klebsiella, staphylococcus, streptococcus and yeasts. the microbial population adher ...	1992	1410807
sealing of occlusal hidden caries lesions: an alternative for curative treatment?	the bacterial composition of the dentine of thirty molars with sealed occlusal surfaces (delton tinted) was examined. undiagnosed hidden caries was present at the time of treatment. although clinically assessed as good (age 3.4 years, s.d. 2.1), 47 percent of the sealants had a marginal defect, when assessed with sem. the dentine of the treated teeth was of a soft consistency. the median of the number of microorganisms was 400 (range 0-3.10(5)) cfu per sample. mutans streptococci and lactobacill ...	1992	1430495
complement activation by lipopolysaccharides purified from gram-negative bacteria isolated from infected root canals.	the purpose of the present study was to investigate the complement activation by lipopolysaccharides purified from porphyromonas (bacteroides) endodontalis, veillonella parvula, and fusobacterium nucleatum isolated from infected root canals. the rate of consumption of the c3t component of the complement increased remarkably when lipopolysaccharides of more than 10 micrograms were added.	1992	1437068
associations between microbial species in dental root canal infections.	the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ...	1992	1494447
bacterial growth inhibition and short-term clinical efficacy of a vegetable oil-based mouthrinse: preliminary study.	bacterial growth of a vegetable oil-based product was determined in vitro. growth of streptococcus mutans and veillonella alcalescens was strongly inhibited for dilutions up to 50x. on the basis of these results, it was decided to evaluate the short-term, clinical efficacy of a 1:10 dilution of the concentrated product in water. fifteen human volunteers employed this rinse twice-a-day during a six-day period. planimetric plaque scores, as well as the plaque and gingival indexes according to löe, ...	1992	1499249
growth yield increase and atp formation linked to succinate decarboxylation in veillonella parvula.	veillonella parvula strain 259 (= dsm 2007) was able to grow on a mineral salts medium supplemented with (per litre) 1 g yeast extract, 1 g tween-80, and 3 mg putrescine.2 hcl, with 6 mm thioglycolate as reductant and lactate as growth substrate. succinate did not serve as a growth substrate, but when added in conjunction with lactate, it was decarboxylated to propionate and resulted in a measurable increase in growth yield, corresponding to the formation of 2.4 g cell dry mass per mol succinate ...	1992	1510569
enumeration of subgingival species on primary isolation plates using colony lifts.	this study evaluated the feasibility of using a colony lift method and dna probes to enumerate bacterial species cultured on primary isolation plates. fourteen digoxigenin-labeled whole chromosomal dna probes representing 12 subgingival species were validated by hybridization with colony lifts prepared from 249 reference strains of 51 species grown on trypticase soy agar plates supplemented with 5% sheep blood. colonies of reference strains were lifted onto nytran filters from plates and treated ...	1992	1528619
the early colonization of gram-negative anaerobic bacteria in edentulous infants.	the occurrence of oral gram-negative anaerobes was examined in 30 edentulous infants (mean age 3 months, range 1-7 months). one pooled swab sample from mucosal surfaces (cheeks, palate, tongue) and one saliva sample was taken from each infant. the samples were cultured aerobically and anaerobically using non-selective and selective media. prevotella (bacteroides) melaninogenica was the most frequently isolated anaerobe, found in 70% of the infants. the other common anaerobes were fusobacterium n ...	1992	1528621
comparative mitogenicity and polyclonal b cell activation capacity of eight oral or nonoral bacterial lipopolysaccharides in cultures of spleen cells from athymic (nu/nu-balb/c) and thymic (balb/c) mice.	optimal stimulatory doses of purified phenol-water extracted lipopolysaccharides (lps) from 5 selected strains of 3 putative periodontopathogens (fusobacterium nucleatum, prevotella intermedia, and veillonella), 3 strains of 2 nonoral bacterial species (bacteroides fragilis and salmonella enteritidis), and pokeweed mitogen (pwm) induced significantly higher maximum mitogenic responses and polyclonal ig production in cultures of unfractionated spleen cells from nu/nu-balb/c (nude) than from balb/ ...	1992	1528635
in vitro hydrogen production by enteric bacteria cultured from children with small bowel bacterial overgrowth.	lactulose breath hydrogen test and enterotest string test were carried out simultaneously on 19 children 3-5 years old. bacteria isolated from the jejunal fluid in upper small intestines of these children were incubated with lactulose at neutral ph. anaerobes were present in all but one child, and in 15 children they were present in numbers greater than 5 log10 organisms per ml. most of these bacteria did not produce hydrogen in vitro. hydrogen production (greater than 100 ppm) was observed with ...	1992	1534365
characterization of veillonella atypica pk1910 adhesin-mediated coaggregation with oral streptococcus spp.	the gram-negative human oral bacterium veillonella atypica pk1910 exhibits both lactose-inhibitable and lactose-noninhibitable coaggregations with certain human oral streptococci. a mild sonication procedure was used to obtain a veillonella surface protein preparation against which antisera were prepared. to characterize the lactose-inhibitable coaggregation, coaggregation-defective (cog-) mutants unable to exhibit this kind of coaggregation (class 1 mutants) were used to absorb the antisera. on ...	1992	1541534
osteomyelitis due to veillonella parvula: case report and review.	anaerobic gram-negative cocci of the genus veillonella are generally normal flora of the oropharynx and upper respiratory tract. when isolated in clinical specimens, these bacteria are usually regarded as commensal organisms rather than as pathogens. we report a case of spinal osteomyelitis in which veillonella parvula was isolated in pure culture from bone as well as blood. osteomyelitis of the spine due to anaerobic bacteria may occur more frequently than has been recognized, and anaerobic cul ...	1992	1571454
modulation of human neutrophil adherence by oral bacteria.	polymorphonuclear neutrophils (pmns) comprise over 90 per cent of leukocytes in the oral cavity. although these phagocytic cells have primary defence roles in the gingiva, their stimulation by micro-organisms may also cause substantial tissue damage due to the release of lysosomal enzymes and oxygen radicals. adherence of pmns to the endothelium and their subsequent diapedesis and egress to areas of infection are considered early vital events in the inflammatory process. in this study, oral bact ...	1992	1605750
pyogenic sacroiliitis due to veillonella parvula.		1992	1617061
modelling of mixed chemostat cultures of an aerobic bacterium, comamonas testosteroni, and an anaerobic bacterium, veillonella alcalescens: comparison with experimental data.	a mathematical model of mixed chemostat cultures of the obligately aerobic bacterium comamonas testosteroni and the anaerobic bacterium veillonella alcalescens grown under dual limitation of l-lactate and oxygen was constructed. the model was based on michaelis-menten-type kinetics for the consumption of substrates, with noncompetitive inhibition of v. alcalescens by o2. the growth characteristics of the aerobic and anaerobic organisms were determined experimentally with pure cultures of the ind ...	1992	1622213
energy conservation by succinate decarboxylation in veillonella parvula.	veillonella parvula cannot grow with succinate as sole energy source. however, succinate decarboxylation simultaneous with malate or lactate fermentation increased growth yields by 2.4-3.5 g (mol succinate)-1. malate was fermented stoichiometrically to acetate and propionate whereas lactate fermentation produced more acetate and considerable amounts of h2. aspartate was utilized only in the presence of succinate as co-substrate. methylmalonyl-coa decarboxylase and atp-dependent pyruvate carboxyl ...	1992	1645132
an examination of strains of the bacterium streptococcus vestibularis for relative cariogenicity in gnotobiotic rats and adhesion in vitro.	the cariogenicity and adhesion of six strains of streptococcus vestibularis were compared with those of strains of strep. salivarius. all strains of strep. vestibularis produced low levels of caries, confined to the fissures, whereas the two strains of strep. salivarius produced high levels of caries, with one strain producing approximal as well as fissure caries. the values for adhesion to saliva-coated hydroxyapatite of strep. vestibularis in the absence (median 4.74%) and the presence (median ...	1991	1651693
relation of baseline microbial parameters to future periodontal attachment loss.	the relationship between the level of subgingival species at baseline and subsequent attachment loss in a subject was examined. 38 subjects (14-71 years) with prior evidence of periodontal destruction were monitored 2x for pocket depth and attachment level at 6 sites per tooth at baseline and 2 months. a subject was considered to exhibit new attachment loss if 1 or more sites increased 3 mm or more in attachment level in 2 months. subgingival plaque samples were taken at the baseline visit from ...	1991	1661304
relation of counts of microbial species to clinical status at the sampled site.	the purpose of the present investigation was to relate clinical characteristics at a site to the frequency of detection, absolute counts and proportions of 14 subgingival species. subgingival plaque samples were removed by curette from the mesial surface of 2299 teeth in 3 healthy and 87 subjects with periodontal attachment loss. samples were dispersed, diluted and plated on trypticase soy agar supplemented with 5% sheep blood. after 7 days of anaerobic incubation, colonies were lifted onto nylo ...	1991	1661305
a comparison of human and animal mouth flora.	human bite injuries are often believed to be more serious than animal bite injuries. one reason for this distinction might be a species difference in the microbial mouth flora. in order to compare this possibility, we cultured the mouths of humans, dogs, and cats. human oral flora contained the smallest number of bacteria followed by dog and cat oral flora, respectively. veillonella parvula was cultured from all humans and was also found in dog and cat mouths, but less frequently. v parvula is n ...	1991	1744782
biological effects of veillonella parvula and bacteroides intermedius lipopolysaccharides.	a comparative study on the endotoxic effects of lipopolysaccharide (lps) from veillonella parvula atcc 10790 and from bacteroides intermedius bmh was performed using an in vivo approach in the c57bl/6 mouse. phenol-water extracted lps of such anaerobes was purified by ultracentrifugation and dnase/rnase digestion, and characterized by a metachromatic assay for endotoxins and by electrophoresis on sds-polyacrylamide gel and silver staining. mouse ld50 for v. parvula lps was 1.479 mg and for b. in ...	1991	1775087
sugar, fluoride, ph and microbial homeostasis in dental plaque.	factors that may contribute to the maintenance or breakdown of the oral microflora have been studied in the laboratory using a mixed culture chemostat system. carbohydrate type had relatively little influence of the proportions of individual species at neutral ph. in contrast, when the ph was allowed to fall following carbohydrate metabolism, the stability of the microflora was markedly perturbed. the proportions of streptococcus mutans, lactobacillus casei and veillonella dispar increased and t ...	1991	1775479
[infective endocarditis and pulmonary abscess in an intravenous drug addict caused by veillonella parvula].		1991	1784772
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis.	to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...	1991	1800380
[effects of antibiotics and anti-bacterial components of preparations for local treatment of suppurative wounds on pathogens of odonto- genic infections].	the results of identification and sensitivity assay of 156 strains of pathogens causing odontogenic infections are presented. in the sensitivity assay antibacterial drugs were used. 42.3 percent of the strains were obligate anaerobes belonging to bacteroides, fusobacterium, peptococcus, peptostreptococcus, veillonella and actinomyces. significant differences in the microbial sensitivity to the drugs used for general and local therapy were detected. there was observed high sensitivity of the obli ...	1991	1805697
bacteria in the apical 5 mm of infected root canals.	ten freshly extracted teeth which had carious pulpal exposures and periapical lesions contiguous with the root apex were placed inside an anaerobic chamber and the apical 5 mm of the root canals cultured. in addition to anaerobic incubation, duplicate cultures were incubated aerobically. fifty strains of bacteria from the 10 root canals were isolated and identified. the most prominent bacteria cultured from the 10 root canals were actinomyces, lactobacillus, black-pigmented bacteroides, peptostr ...	1991	1809801
predominant cultivable flora in pericoronitis.	the predominant cultivable flora in pericoronitis was investigated by culturing pus from affected sites in 20 patients. twenty colonies were picked at random from non-selective plates and identified using conventional biochemical and physiological tests, analysis of metabolic end-products by gas chromatography and protein profile analysis. the most frequently isolated organisms were prevotella (bacteroides) intermedia, peptostreptococcus micros, veillonella species, fusobacterium nucleatum and s ...	1991	1820571
microbial risk indicators for periodontal attachment loss.		1991	1831856
[oral bacterial flora and its pathogenic potential].	the oral flora is a complex body formed by at least a hundred bacterial species which can be more or less regular residents in the oral cavity. thanks to a developing taxonomy related to progress made in bacterial identification, these oral species can be separated into five groups based gram staining and morphology: the gram positive cocci group (streptococcus, staphylococcus and related geni), the gram negative cocci group (neisseria, veillonella and related geni), the gram positive bacilli gr ...	1991	1864855
swedish study group. a randomized multicenter trial to compare the influence of cefaclor and amoxycillin on the colonization resistance of the digestive tract in patients with lower respiratory tract infection.	eighty-four patients with lower respiratory tract infections participated in a randomised double-blind parallel multicenter trial in order to compare the efficacy of cefaclor and amoxycillin as treatment for lower respiratory tract infections and their ability to influence colonization resistance. cefaclor was given to 40 patients and amoxycillin to 44 patients perorally in doses of 250 mg t.i.d. for seven days in a double-blind fashion. sputum, oropharyngeal and intestinal specimens were taken ...	1991	1917031
[pleural empyema and ruptured subdiaphragmatic abscess].	the authors report a case of a ruptured right subphrenic abscess causing a diffuse peritonitis complicating a chronic empyema. the authors recommend active treatment of purulent pleurisies, particularly when they are associated with a bronchopleural fistula.	1991	1924980
specificity of antibodies present in human periapical lesions.	various classes of immunoglobulins have been found in human periapical lesions. the specificity of secreted antibodies against antigens egressing from the root canal system has yet to be thoroughly investigated. the purpose of this study was to test the specificity of antibodies present in human periapical lesions. human periapical biopsies were removed and cultured as organ culture explants. antibodies present in the lesions were extracted in the cell culture fluids. a modified enzyme-linked im ...	1991	1940740
a multi-station dental plaque microcosm (artificial mouth) for the study of plaque growth, metabolism, ph, and mineralization.	a plaque growth chamber was developed for long-term growth of five separate plaques from the same plaque or saliva sample under identical conditions of temperature and gas phase. reagent addition and growth conditions for each plaque could be independently controlled, and each was accessible for sequential sampling and electrode insertion. plaques were cultured for over six weeks on pellicle-coated lux (tm) 25-mm diameter cover-slips at 35 degrees c under 5% co2 in n2, and supplied with a medium ...	1991	1960250
[anaerobic bacteria and their role in recurrent tonsillitis].	the presence of anaerobic bacteria in 100 palatal tonsils obtained during the tonsillectomy, was evaluated in quality and in quantity. 17 species of anaerobic bacteria were found. the most frequent were: bacteroides melaninogenicus, peptostreptococcus anaerobius, peptostreptococcus intermedius, propionibacterium, veillonella parvula and peptococcus asaccharolyticus. in most evaluations their density was 10(5)-10(6) in gram of tonsil tissue, sometimes 10(7). its role in recurrent tonsils infectio ...	1990	1981606
t cell proliferative responses to molecular fractions of periodontopathic bacteria.	soluble antigenic preparations of veillonella parvula and bacteroides gingivalis were separated by sds-page and used after electroblotting and solubilization for in vitro lymphocyte stimulation in 13 patients with severe periodontitis and 12 controls. the cellular responses of controls and patients to v. parvula antigens were represented by four main proliferation-inducing fractions with 74-66, 52-46, 22-19 and 12 kd mol. wt. these fractions induced slightly enhanced dna synthesis in lymphocytes ...	1991	1988218
on the mechanism of sodium ion translocation by methylmalonyl-coa decarboxylase from veillonella alcalescens.	veillonella alcalescens during lactate degradation developed an na+ concentration gradient with 7-8 times higher external than internal na+ concentrations in the logarithmic growth phase. the gradient declined to a factor of 1.9 in the late stationary phase. methylmalonyl-coa decarboxylase reconstituted into proteoliposomes performed an active electrogenic na+ transport, creating delta psi of 60 mv, delta pna+ of 50 mv, and delta mu na+ of 110 mv. in the initial phase of the transport, the decar ...	1991	1991479
oral associated bacterial infection in horses: studies on the normal anaerobic flora from the pharyngeal tonsillar surface and its association with lower respiratory tract and paraoral infections.	two hundred and seventy bacterial isolates were obtained from the pharyngeal tonsillar surface of 12 normal horses and 98 obligatory anaerobic bacteria were characterised. of these, 57 isolates belonging to 7 genera (peptostreptococcus (1); eubacterium (9); clostridium (6); veillonella (6); megasphera (1); bacteroides (28); fusobacterium (6)) were identified, and 16 of these were identified to species level (p. anaerobius (1); e. fossor (9); c. villosum (1); b. fragilis (1); b. tectum (2); b. he ...	1991	2031304
prediction of caries increment in scottish adolescents.	salivary, clinical, and microbiological factors were compared with subsequent 2-yr caries increment in a group of 372 scottish adolescents. the caries increment was significantly correlated with previous caries experience, salivary buffering capacity and counts of lactobacilli, mutans streptococci and candida. veillonella levels and salivary flow rate were not correlated with caries increment. significant improvements in the predictions were obtained when the results of more than one test were i ...	1991	2049927
effect of nutrients on defined bacterial plaques and streptococcus mutans c67-1 implantation in a model mouth.	actinomyces viscosus wvu 627, streptococcus oralis lpa-1 and veillonella dispar omz 193 were cocultured on teeth in a model mouth for 66 h. synthetic saliva containing bovine salivary glycoprotein supported bacterial growth, although the delivery of an intermittent nutrient supplement, containing 1% (w/v) glucose or sucrose, gave greater bacterial cell and viable counts. when streptococcus mutans c67-1 was super-inoculated onto 24-hour mixed plaques, it became established under all regimens, but ...	1991	2059972
comparison of the plaque microflora from natural and appliance-borne enamel surfaces.	human enamel sections and slabs, mounted on a mandibular removable appliance, were worn by 5 adult subjects for a 1-week period. plaque was allowed to accumulate on the in situ test sites and on the adjacent natural dentition. at the end of the experimental period, the plaque microflora associated with (1) the enamel sections, (2) the enamel slabs, and (3) the acrylic base of the appliance test site was compared with that obtained from lingual and interproximal areas of the lower molar teeth. in ...	1991	2070382
sensitivity of oral bacteria to imipenem and other antibiotics.	specimens from a variety of oral and maxillofacial infections were cultured and 88 bacterial isolates belonging to the genera bacteroides, actinomyces, staphylococcus, veillonella, eubacterium, streptococcus and aerococcus were obtained. these were tested for their sensitivity to imipenem, penicillin, cefuroxime, vancomycin, erythromycin and fusidic acid. imipenem proved to be the most effective of the antibiotics tested, inhibiting all 88 isolates. penicillin was the least effective in that it ...	1990	2084496
an in vitro method to study the adherence of bacteria to saliva-treated tooth enamel sections.	an in vitro bacterial adherence assay which employed human tooth enamel sections precoated with saliva and an epifluorescent staining technique with acridine orange was developed. the assay was used to study the adherence properties of fresh and type strains of the following oral bacterial species: bacteroides gingivalis, bacteroides intermedius, capnocytophaga species, haemophilus aphrophilus, actinobacillus actinomycetemcomitans, peptostreptococcus species, veillonella species, actinomyces isr ...	1990	2087341
isolation and characterization of coaggregation-defective mutants of veillonella atypica.	spontaneously occurring coaggregation-defective mutants of veillonella atypica pk1910 were isolated and used to study the interactions of veillonella with streptococcus sanguis, strep. gordonii and strep. sm. three classes of mutants were isolated. the first class failed to participate in lactose-inhibitable coaggregations with streptococcal cells. the second class of mutants failed to coaggregate by the lactose-non-inhibitable interactions. the third group, isolated from the first group, failed ...	1990	2088215
effect of granulocytopenia on oral microbial relationships in patients with acute leukemia.	risk for acute infection increases as granulocyte levels decrease secondary to myelosuppressive chemotherapy in patients with acute nonlymphocytic leukemia (anll). acute exacerbations of concomitant inflammatory periodontal diseases can result in systemic infections in these patients. however, host-oral bacterial relationships in the periodontium in patients with anll are not well understood. twenty-one adult patients with anll with periodontal disease ranging from gingivitis to severe periodont ...	1990	2124667