Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [enteral yersiniosis--a serious disease? current knowledge of clinical aspects, diagnosis and therapy]. | enteral yersiniosis is caused either by y. enterocolitica 0-group i (syn. serotype 0:3) and 0-group v (syn. serotype 0:9) or y. pseudotuberculosis type i-vi. the clinical symptoms are mostly like enteritis, enterocolitis, acute abdomen, mesenteric lymphadenitis, or ileitis terminalis. post-infection reactions are possible like septicemia, arthritis and erythema nodosum. only cultural and serological examinations confirm the diagnosis of enteral yersiniosis. in the judgement of serological result ... | 1982 | 7095694 |
| [attempts of ultrastructural and biochemical characterisation of cell wall deficient "brucella" (l forms) (author's transl)]. | in previous studies the in vivo conversion of brucella suis to l-form state was put in evidence. the l forms isolated from mouse spleen had original structural aspects in common: the absence of the cell wall layer and the extracellular multilayer "membranous" structures. the biological characterization of these l forms and the preliminary identification of specific chemical markers of the bacterial envelope is reported in the present study, performed with the stable l forms well-growing in the l ... | 1982 | 7103308 |
| brucella suis: an unusual cause of suppurative lymphadenitis in an outpatient. | a routine aerobic culture of purulent material from a draining sinus tract of a patient with chronic lymphadenitis yielded growth of a fastidious gram-negative coccobacillus later identified as brucella suis biotype 1. the patient responded to administration of antibacterial drugs and surgical drainage. | 1982 | 7130371 |
| [development of brucella abortus, brucella melitensis and brucella and brucella suis infection infection in mice to a chronic stage and cure]. | 1982 | 7168538 | |
| brucellosis in wild swine: a serologic and bacteriologic survey in the southeastern united states and hawaii. | sera from wild swine from 20 populations in 11 southeastern states and hawaii were tested for brucellosis by the card, tube, plate, buffered plate antigen, complement-fixation, and rivanol tests. twenty-one of 352 (6.0%) swine were considered seropositive, and these animals originated from 7 populations in 5 states: arkansas, florida, georgia, louisiana, and south carolina. cultural examinations for brucella suis also were made from subsamples of the swine tested serologically. brucella suis bio ... | 1982 | 7174444 |
| experimental brucella suis type 4 infections in domestic and wild alaskan carnivores. | beagle dogs were readily infected by 1.3 x 10(8) colony forming units (cfu) of brucella suis type 4 administered either on canned dog food, or intraperitoneally. such infections were afebrile and otherwise asymptomatic and without any obvious gross lesions. inoculation of 10(8) cfu b. suis type 4 intraperitoneally into two gravid wolves (canis lupus) resulted in infections in both animals. about 24 days later they gave birth, apparently at full-term, to two (both alive) and six (two alive and fo ... | 1981 | 7241703 |
| cellular fatty acids of brucella canis and brucella suis. | the cellular fatty acid composition of brucella canis and brucella suis was determined by gas-liquid chromatography. the presence of relatively large amounts of a 19-carbon cyclopropane fatty acid in b. suis was a major distinguishing feature between these organisms. the gas-liquid chromatography test for cellular fatty acids provides an additional criterion for the distinction of antigenically rough strains of b. suis which cannot be differentiated from b. canis by conventional procedures. | 1981 | 7263850 |
| isolation of l-forms from the spleens of brucella suis-infected, penicillin-treated mice. | previous attempts to obtain in vitro wall-deficient stable l-forms of various strains of brucella have failed because the obtained spheroplasts revert quickly to bacterial form. here, we report the isolation of l-forms from mice infected with a b. suis strain type 1 and treated with penicillin. in defined experimental conditions, l-type microcolonies associated with tissue debris were observed in primary spleen cultures, even on antibiotic free media. after several transfers on penicillin-contai ... | 1981 | 7294609 |
| [hepatic calcifications in isolated necrotizing, granulomatous hepatitis due to brucella. a case report and review of the literature (author's transl)]. | a case of isolated necrotizing, granulomatous hepatitis of brucella origin is discussed, together with 6 similar cases reported in the published literature. the clinical picture is dominated by the history of the patient (occupation, geographical location), fever, right hypochondrial pain, and altered general condition. radiological examination shows characteristic calcifications. diagnosis is confirmed by serology, brucella suis usually being involved. pathological appearances are typical : lar ... | 1981 | 7320978 |
| experimental infections by brucella suis type 4 in alaskan rodents. | the susceptibility of nine species of rodents and one species of lagomorph to brucella suis type 4 was studied experimentally. the rodent species included: guinea pig (cavia porcellus), scandinavian lemming (lemmus lemmus), brown lemming (l. sibiricus), northern red-backed vole (clethrionomys rutilis), varying lemmings (dicrostonyx stevensoni and d. rubricatus), yellow-cheeked vole (microtus xanthognathus), flying squirrel (glaucomys sabrinus) and ground squirrel (citellus parryii). the lagomorp ... | 1980 | 7463596 |
| effect of rifampicin and tetracycline alone and in combination against brucella suis. | in vivo and in vitro efficacy of rifampicin and tetracycline hydrochloride, used alone and in combination, was tested against one strain of brucella suis. the minimal inhibitory concentrations of rifampicin and tetracycline hydrochloride against this strain were 2 and 0.2 microgram/ml, respectively. in vitro efficacy was evaluated by inhibition of bacterial growth in liquid medium. rifampicin killed 99.9% of the brucella after 18 h of incubation of concentrations of 2 and 4 microgram/ml. tetracy ... | 1980 | 7469256 |
| dissociation of delayed-type hypersensitivity from acquired murine resistance to brucella suis. | mouse pretreatment with cyclophosphamide or acetoacetylation of the vaccinating fraction "pi" lead to a dissociation between enhanced anti-pi dth responses and normal or depressed acquired resistance to an infective challenge with brucella suis. | 1980 | 7469385 |
| restriction site polymorphism of the genes encoding the major 25 kda and 36 kda outer-membrane proteins of brucella. | seventy-seven brucella reference and field strains from different geographic origins and hosts representing the six recognized species and their different biovars were analysed for diversity of their genes encoding the major 25 and 36 kda outer-membrane proteins (omps) by pcr-rflp. the 25 kda omp is encoded by a single gene (omp25) whereas two closely related genes (omp2a and omp2b) encode and potentially express the 36 kda omp. analysis of pcr products of the omp25 gene digested with nine restr ... | 1995 | 7496522 |
| comparison of serologic tests and bacteriologic culture for detection of brucellosis in swine from naturally infected herds. | to compare results of 6 serologic tests with results of bacteriologic culture for brucella suis in swine. | 1995 | 7591930 |
| comparison of the efficacy of brucella suis strain 2 and brucella melitensis rev. 1 live vaccines against a brucella melitensis experimental infection in pregnant ewes. | the comparative efficacy of brucella suis strain 2 (s2) and brucella melitensis strain rev. 1 (rev. 1) live vaccines in protecting sheep against b. melitensis infection was evaluated by clinical and bacteriological examination of ewes vaccinated conjunctivally with a dose of 1 x 10(9) c.f.u. when 4 months old and then challenged with 5 x 10(7) c.f.u. of the b. melitensis virulent strain 53h38 (h38) at the middle of the first or second pregnancy following vaccination. animals were considered to b ... | 1995 | 7625115 |
| [spondylodiscitis cause by brucella suis]. | in a 72-year-old dutchman brucellae suis spondylodiscitis txii-li was diagnosed, complicated by a paraspinal abscess, for which surgical drainage was carried out. the patient failed to recover after the combined antibiotic regimen of doxycycline, rifampicin and trimethoprim-sulfamethoxazole. there was good clinical improvement after a course of doxycycline and streptomycin, however. after a symptom-free period of 9 weeks a relapse occurred. the patient refused treatment and died 6 weeks later at ... | 1994 | 7808527 |
| differentiation of brucella abortus bv. 1, 2, and 4, brucella melitensis, brucella ovis, and brucella suis bv. 1 by pcr. | several pcr assays which identify the genus brucella but do not discriminate among species have been reported. we describe a pcr assay that comprises five oligonucleotide primers which can identify selected biovars of four species of brucella. individual biovars within a species are not differentiated. the assay can identify three biovars (1, 2, and 4) of b. abortus, all three biovars of b. melitensis, biovar 1 of b. suis, and all b. ovis biovars. these biovars include all of the brucella specie ... | 1994 | 7852552 |
| differentiated u937 cells exhibit increased bactericidal activity upon lps activation and discriminate between virulent and avirulent listeria and brucella species. | in the study of interactions between facultative intracellular pathogens and macrophages, monocytic cell lines have the advantages of showing defined states of activation and lacking genetic variation among donors, thus yielding reproducible results. nonpathogenic escherichia coli k12 were killed at similar rates in the u937 cell line differentiated into macrophage-like cells by phorbol myristate acetate (pma) or by the combination of retinoic acid (ra) and vitamin d3 (vd). complete elimination ... | 1994 | 8071593 |
| protein profiles of brucella suis and brucella abortus in isoelectric focusing and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. | the protein profiles of five field isolates of brucella suis and four field isolates of b. abortus were examined. isoelectric focusing of soluble proteins from cell sonicates produced 28 bands common to both species, with a further 10 bands being unique to a species. no intraspecies variation was detected. sodium dodecyl sulfate extracted proteins were resolved by polyacrylamide gel electrophoresis. the proteins were transferred to nitrocellulose and then stained. these techniques allowed the de ... | 1994 | 8203125 |
| immunoblot studies in the differential diagnosis of porcine brucellosis: an immunodominant 62 kda protein is related to the mycobacterial 65 kda heat shock protein (hsp-65). | smooth brucella spp. share certain lipopolysaccharide antigens with other bacteria, resulting in serological cross-reactions which can prevent the definitive diagnosis of brucellosis. to identify other antigens with serodiagnostic potential, immunoblot studies following sodium dodecyl sulphate-polyacrylamide gel electrophoresis were carried out. sera from pigs experimentally infected with brucella suis and naturally infected feral pigs, sera from pigs from a farm with a known history of yersinia ... | 1994 | 8203127 |
| naturally occurring brucella suis biovar 4 infection in a moose (alces alces). | a debilitated adult female moose (alces alces) shot east of the mackenzie river, northwest territories, canada, had large fluctuant masses over both carpi. only the forelimbs were available for examination. carpal pathology included bilateral bursitis and osteomyelitis of subjacent bone. in addition severe osteomyelitis with fractures was observed in the left lateral and right medial digits. brucella suis biovar 4 was isolated from the right medial first phalanx. this is believed to be the first ... | 1993 | 8258861 |
| field-oriented trial of the chinese brucella suis strain 2 vaccine on sheep and goats in libya. | the chinese brucella suis s2 vaccine was studied in a flock of sheep and goats in field conditions. the locally-produced vaccine was orally administered in the form of a drench to 446 ewes, 50 lambs and 20 adult goats. after vaccination, abortion and excretion of the vaccine strain in vaginal discharges or in milk did not occur. serological tests became positive in 92% of animals at 4 wk post-vaccination and declined to near nil after 1 yr. the protection conferred by the vaccine against a doubl ... | 1993 | 8260964 |
| efficacy of brucella suis strain 2 vaccine against brucella ovis in rams. | the protective efficacy against brucella ovis of live vaccine brucella suis strain 2 (s2) and brucella melitensis strain rev 1 has been evaluated in rams. fourteen 4-month-old brucella-free aragonesa rams were vaccinated conjunctivally with 2 x 10(9) c.f.u. s2. sixteen rams of the same breed, condition and age were conjunctivally vaccinated the same day with 1.6 x 10(9) rev 1. thirteen rams were unvaccinated controls. eight months after vaccination all rams were challenged with 6 x 10(9) c.f.u. ... | 1993 | 8296481 |
| brucellosis: re-emergence and changing epidemiology in queensland. | to define the epidemiological and clinical characteristics of human brucellosis, which is being detected in increasing numbers of patients in queensland. | 1993 | 8336614 |
| transmission of brucellosis from reindeer to cattle. | sixteen reindeer (rangifer tarandus tarandus) naturally infected with brucella suis biovar 4 were penned with 6 male and 2 female cattle for 30 days, then removed and euthanatized. during this period, 5 reindeer had fawns, and 2 reindeer aborted. brucella suis biovar 4 was recovered from all adult reindeer at necropsy. nine reindeer had b suis biovar 4 in uterus, udder, and/or milk. the cattle were euthanatized 2 months after the reindeer were removed. clinical or pathologic signs of disease wer ... | 1993 | 8407493 |
| isolation of brucella suis from the semen of a ram. | 1993 | 8430486 | |
| serological reactions to brucella species in british pigs. | until and including 1987 diagnostically significant serological titres to swine brucellosis had occurred in the serum agglutination test (sat) or the complement fixation test (cft), ie, > or = 100 iu or > or = 20 icftu, respectively, almost every year since reliable records began, but usually only about 0.05 per cent in the sat and 0.005 per cent in the cft. brucella suis was never isolated by cultural examination. in 1988 the level of cft reactions > or = 20 icftu rose to 0.42 per cent (1.04 pe ... | 1993 | 8517004 |
| distribution of annexin i during non-pathogen or pathogen phagocytosis by confocal imaging and immunogold electron microscopy. | annexin i is an abundant protein in u937 cells differentiated towards a macrophagic phenotype. these cells become able to kill escherichia coli, however, the intracellular pathogen brucella suis, known to interfere with phagosome maturation, multiply in these differentiated cells. we have analysed by confocal and electron microscopy the cellular localization of annexin i during phagocytosis of yeast, non-pathogenic e. coli and the intracellular pathogen b. suis. using immunocytochemical detectio ... | 1996 | 8673068 |
| nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of brucella ovis: evidence for antigenic shift, compared with other brucella species, due to a deletion in the gene. | the nucleotide sequences encoding the major 25-kda outer membrane protein (omp) (omp25 genes) of brucella ovis 63/290, brucella melitensis 16m, brucella suis 1330, brucella canis rm6/66, and brucella neotomae 5k33 (all reference strains) were determined and compared with that of brucella abortus 544 (p. de wergifosse, p. lintermans, j. n. limet, and a. cloeckaert, j. bacteriol. 177:1911-1914, 1995). the major difference found was between the omp25 gene of b. ovis and those of the other brucella ... | 1996 | 8675306 |
| cloning of a brucella melitensis group 3 antigen gene encoding omp28, a protein recognized by the humoral immune response during human brucellosis. | brucella group 3 antigens (ags) are outer membrane proteins (omps) with a molecular mass ranging from 25 to 30 kda. the omps are of interest partially because of their potential use as vaccine and diagnostic reagents. we used human convalescent antibody (ab) to clone a gene that encoded a 28-kda protein from a lambdagt11 library of brucella melitensis 16m genomic dna. dna sequence analysis revealed a single open reading frame that would encode a protein of 26,552 da. the 28-kda protein had a pri ... | 1996 | 8698471 |
| protection of balb/c mice against homologous and heterologous species of brucella by rough strain vaccines derived from brucella melitensis and brucella suis biovar 4. | to evaluate stable rough mutants derived from brucella melitensis 16m and b suis 2579 (biovar 4) as vaccines against homologous and heterologous brucella spp in the balb/c mouse model. design, animals, and procedure: rough mutants vtrm1 and vtrs1 were obtained from b melitensis 16m and b suis 2579, respectively, by allelic exchange of rfbu gene encoding mannosyltransferase with a tn5-disrupted rfbu gene. mice were vaccinated with vtrm1 or vtrs1 and challenge exposed 8 weeks later. | 1996 | 8723881 |
| participation of the molecular chaperone dnak in intracellular growth of brucella suis within u937-derived phagocytes. | in the intracellular bacterium brucella suis, the molecular chaperone dnak was induced under heat-shock conditions and at low ph. insertional inactivation of dnak and dnaj within the dnak/j locus led to the conclusion that dnak, but not dnaj, was required for growth at 37 degrees c in vitro. viability of the dnak null mutant was also greatly affected at low ph. under conditions allowing intracellular multiplication, the infection of u937-derived phagocytes resulted in long-lasting dnak induction ... | 1996 | 8793869 |
| serological and bacteriological study of swine brucellosis. | a serological and bacteriological study was performed with sera taken from 2,228 swine from six states in venezuela. none of the animals were vaccinated against brucellosis, and the prevalence of the disease varied from 5 to 89% on farms located in these states. our studies indicated that the animals could be categorized into four groups depending on the degree of reactivity in serological tests. brucella suis biovar 1 was isolated from the lymph nodes, spleens, and semen samples of seropositive ... | 1997 | 8968931 |
| brucellosis: an overview. | brucellosis remains a major zoonosis worldwide. although many countries have eradicated brucella abortus from cattle, in some areas brucella melitensis has emerged as a cause of infection in this species as well as in sheep and goats. despite vaccination campaigns with the rev 1 strain, b. melitensis remains the principal cause of human brucellosis. brucella suis is also emerging as an agent of infection in cattle, thus extending its opportunities to infect humans. the recent isolation of distin ... | 1997 | 9204307 |
| rangiferine brucellosis on baffin island. | the standard tube agglutination test (stat) and the complement fixation test (cft) were used to assess the seroprevalence of antibodies to brucella spp. in caribou (rangifer tarandus) from three populations on baffin island, canada. during late winter from 1983 to 1986, sera from 17 of 40 north baffin (43%), 11 of 33 northeast baffin (33%) and 12 of 82 south baffin (15%) adult caribou had antibodies in the stat at 1:50 or the cft at 1:5. seroprevalence increased as caribou matured with one (4%) ... | 1997 | 9249700 |
| dna polymorphism at the omp-31 locus of brucella spp.: evidence for a large deletion in brucella abortus, and other species-specific markers. | the omp-31 gene, encoding a major outer-membrane protein in brucella melitensis, was pcr-amplified from brucella strains representing all species and known biovars by using primers selected according to the b. melitensis 16m omp-31 published sequence. amplification of omp-31 was achieved from dna of all brucella species with the exception of brucella abortus, the only brucella species where expression of omp-31 was not detected by reactivity with an mab specific for an epitope located in omp-31. ... | 1997 | 9308175 |
| the pathogenicity of brucella suis biovar 4 for bison. | the pathogenicity of brucella suis biovar 4 for bison (bison bison) was evaluated by inoculation of 2.1 x 10(7) colony forming units (cfu) in 0.1 ml saline into the conjunctival sac of six pregnant cows. six pregnant bison were inoculated with 1.27 x 10(7) cfu of brucella abortus strain 2308 as a positive control. bison were inoculated on 23 january 1992, and observed until calving or abortion after which they were euthanized, and necropsied. bacteriological and histological examinations were co ... | 1996 | 9359055 |
| brucella suis biovar 1 in naturally infected cattle: a bacteriological, serological, and histological study. | 1997 | 9376434 | |
| differences in chromosome number and genome rearrangements in the genus brucella. | we have studied the genomic structure and constructed the spei, paci and i-ceui restriction maps of the four biovars of the pathogenic bacterium brucella suis. b. suis biovar 1 has two chromosomes of 2.1 mb and 1.15 mb, similar to those of the other brucella species: b. melitensis, b. abortus, b. ovis and b. neotomae. two chromosomes were also observed in the genome of b. suis biovars 2 and 4, but with sizes of 1.85 mb and 1.35 mb, whereas only one chromosome with a size of 3.1 mb was found in b ... | 1998 | 9466259 |
| the public health risks associated with wild and feral swine. | wild swine populations (sus scrofa) are present in many regions of the world. large feral populations in north america and australia are principally derived from introduced domestic pigs. in europe, most wild boar are found in germany and poland. while wild swine are certainly a significant reservoir of infection for domestic swine diseases (for example, african swine fever virus in wild boar in sardinia), these swine generally do not constitute a major public health risk. brucella suis infectio ... | 1997 | 9501373 |
| expression and bactericidal activity of nitric oxide synthase in brucella suis-infected murine macrophages. | we examined the expression and activity of inducible nitric oxide synthase (inos) in both gamma interferon (ifn-gamma)-treated and untreated murine macrophages infected with the gram-negative bacterium brucella suis. the bacteria were opsonized with a mouse serum containing specific antibrucella antibodies (ops-brucella) or with a control nonimmune serum (c-brucella). the involvement of the produced no in the killing of intracellular b. suis was evaluated. b. suis survived and replicated within ... | 1998 | 9529047 |
| identification, cloning and initial characterisation of feupq in brucella suis: a new sub-family of two-component regulatory systems. | to cause disease, brucella species have to adapt to a range of different environments. environmental sensing and adaptive responses in bacteria often involve the concerted action of a two-component regulatory system, consisting of sensor and response regulator components. amplification and sequence analysis of response regulators from brucella species identified a response regulator sequence with 96% similarity to rhizobium leguminosarum feup. in r. leguminosarum, the feupq two-component system ... | 1998 | 9595675 |
| response of brucella suis 1330 and b. canis rm6/66 to growth at acid ph and induction of an adaptive acid tolerance response. | acid ph is an environmental stress often encountered by brucella during both the "environmental" and the "pathogenic" stages of its life. we have investigated the behaviour of b. suis biovar 1 and b. canis in acid conditions. growth at suboptimal ph was characterized by a dramatic reduction in growth yield due to an early onset of stationary phase. b. suis was more resistant to low ph than b. canis, which lysed at ph 4.6. viable counts measured after a 4-h acid shock at ph 3.2 showed that the re ... | 1997 | 9765795 |
| identification of brucella by ribosomal-spacer-region pcr and differentiation of brucella canis from other brucella spp. pathogenic for humans by carbohydrate profiles. | molecular and chemical characteristics often provide complementary information in the differentiation of closely related organisms. the genus brucella consists of a highly conserved group of organisms. identification of the four species pathogenic in humans (brucella melitensis, brucella abortus, brucella suis, and brucella canis) is problematic for many clinical laboratories that depend primarily on serology and phenotypic characteristics to differentiate species. pcr amplification of the 16s-2 ... | 1998 | 9774568 |
| differentiation of microorganisms based on pyrolysis-ion trap mass spectrometry using chemical ionization. | the ability to differentiate microorganisms using pyrolysision trap mass spectrometry was demonstrated for five gram-negative disease-causing organisms: brucella melitensis, brucella suis, vibrio cholera, yersinia pestis, and francisella tularensis. bacterial profiles were generated for gamma-irradiated bacterial samples using pyrolytic methylation and compared for electron ionization and chemical ionization using several liquid reagents with increasing proton affinities. electron ionization com ... | 1999 | 9989380 |
| [brucellosis and aujeszky's disease in a wild boar enclose. case report]. | in wild boars kept in a paddock of approximately 1.7 square miles brucella suis biotype 2 was isolated and aujeszky's disease was diagnosed by serological tests. to clear the paddock, the wild boars were lured by offered food and caught using specially built smaller pens. they were then transported to a location far off their original surroundings and euthanized. of a total of 297 wild boars kept in the paddock, 71% were caught and 28% shot. three wild boars (= 1%) were found trapped. the descri ... | 1999 | 10077814 |
| the elisa for the examination of hare sera for anti-brucella antibodies. | hare brucellosis is caused primarily by brucella suis biovar 2. hares along with wild boars are the natural reservoir of this microorganism. in view of restriction of applicability of traditional serological methods the work aimed to develop the elisa to examine hare sera for the presence of anti-brucella antibodies. lipopolysaccharide (lps) antigen obtained from the strain s19 of brucella abortus and the conjugate of antibodies against rabbit immunoglobulin with horseradish peroxidase were used ... | 1999 | 10099027 |
| experimental infection of white-tailed deer with rangiferine brucellosis. | experimental infections of white-tailed deer (odocoileus virginianus) with brucella suis biovar 4 were evaluated over a period of 6 wk. five adult male hand-raised white-tailed deer were inoculated with 1 x 10(7) colony forming units of b. suis biovar 4 in the conjunctiva and serologically evaluated over 6 wk by the card test (card), rivanol test (riv), serum agglutination test (sat), complement fixation test (cft), particle concentration fluorescence immunoassay (pcfia), and competitive enzyme ... | 1999 | 10231769 |
| investigation into the role of the response regulator ntrc in the metabolism and virulence of brucella suis. | during infection, brucella species have to adapt to a range of different environments. environmental sensing in bacteria often involves the concerted action of two-component regulatory systems consisting of sensor and response regulator components. in this study, we identified, cloned and sequenced four independent response regulator gene fragments from brucella melitensis. one amplified gene fragment showed nearly 90% identity to the response regulator subfamily of ntrc transcriptional activato ... | 1999 | 10373105 |
| early acidification of phagosomes containing brucella suis is essential for intracellular survival in murine macrophages. | brucella suis is a facultative intracellular pathogen of mammals, residing in macrophage vacuoles. in this work, we studied the phagosomal environment of these bacteria in order to better understand the mechanisms allowing survival and multiplication of b. suis. intraphagosomal ph in murine j774 cells was determined by measuring the fluorescence intensity of opsonized, carboxyfluorescein-rhodamine- and oregon green 488-rhodamine-labeled bacteria. compartments containing live b. suis acidified to ... | 1999 | 10417172 |
| use of western immunoblot analysis for testing moose serum for brucella suis biovar 4 specific antibodies. | to determine if 12 moose (alces alces) from northern alaska with agglutinating antibodies specific for brucella spp. had been exposed to either b. suis biovar 4 or b. abortus biovar 1, western immnnoblot serologic analysis was performed. differential serologic responses to strain specific a and m antigenic variances of the lipopolysaccharide o-polysaccharide sugar allowed strain identification. prior to examination, test sera were absorbed with killed whole cells from either b. abortus biovar 1, ... | 1999 | 10479098 |
| validation of the fluorescence polarization assay as a serological test for the presumptive diagnosis of porcine brucellosis. | sera from canadian pigs (brucellosis free, n = 14037) and sera from pigs infected with brucella suis (n = 401) were tested by the buffered antigen plate agglutination test, the complement fixation test, an indirect and a competitive enzyme immunoassay and a fluorescence polarization assay. the results were analysed and assay sensitivity and specificity estimates were calculated. the sensitivity and specificity of the tests were as follows: the buffered antigen plate agglutination test, 77.1 and ... | 1999 | 10510043 |
| a homologue of the agrobacterium tumefaciens virb and bordetella pertussis ptl type iv secretion systems is essential for intracellular survival of brucella suis. | analysis of a tnblam mutant of brucella suis 1330, identified as being unable to multiply in hela cells, allowed us to identify a 11 860 bp region of the b. suis genome encoding a type iv secretion system, homologous to the virb system of agrobacterium tumefaciens and the ptl system of bordetella pertussis. dna sequence revealed 12 open reading frames (orfs) encoding homologues of the 11 virb proteins present in the pti plasmid of agrobacterium with a similar genetic organization, and a twelfth ... | 1999 | 10510235 |
| constitutive and inducible expression of green fluorescent protein in brucella suis. | a gene fusion system based on plasmid pbbr1mcs and the expression of green fluorescent protein was developed for brucella suis, allowing isolation of constitutive and inducible genes. bacteria containing promoter fusions of chromosomal dna to gfp were visualized by fluorescence microscopy and examined by flow cytometry. twelve clones containing gene fragments induced inside j774 murine macrophages were isolated and further characterized. | 1999 | 10569794 |
| in vitro brucella suis infection prevents the programmed cell death of human monocytic cells. | during the complex interaction between an infectious agent and a host organism, the pathogen can interfere with the host cell's programmed death to its own benefit. induction or prevention of host cell apoptosis appears to be a critical step for determining the infection outcome. members of the gram-negative bacterial genus brucella are intracellular pathogens which preferentially invade monocytic cells and develop within these cells. we investigated the effect of brucella suis infection on apop ... | 2000 | 10603407 |
| a screening elisa for brucellosis in reindeer. | an enzyme-linked immunosorbent assay (elisa) for the screening of brucellosis in reindeer was developed. the assay, which utilizes s-lps from brucella abortus as antigen and biotin-labelled rabbit antibody to reindeer immunoglobulin as detecting antibody, has a high specificity and sensitivity, as indicated in a validation with sera from reindeer cultured positive for brucella suis biovar 4 and sera from reindeer free of brucellosis. | 1999 | 10605375 |
| identification of brucella suis genes affecting intracellular survival in an in vitro human macrophage infection model by signature-tagged transposon mutagenesis. | bacteria of the genus brucella are facultative intracellular pathogens which have developed the capacity to survive and multiply in professional and nonprofessional phagocytes. the genetic basis of this aspect of brucella virulence is still poorly understood. to identify new virulence factors, we have adapted signature-tagged transposon mutagenesis, which has been used essentially in animal models, to an in vitro human macrophage infection model. a library of 1,152 brucella suis 1330 tagged mini ... | 2000 | 10678941 |
| a beneficial aspect of a cb1 cannabinoid receptor antagonist: sr141716a is a potent inhibitor of macrophage infection by the intracellular pathogen brucella suis. | the psychoactive component of marijuana, delta9-tetrahydrocannabinol (thc) suppresses different functions of immunocytes, including the antimicrobicidal activity of macrophages. the triggering of cannabinoid receptors of cb1 and cb2 subtypes present on leukocytes may account for these effects. we investigated the influence of specific cb1 or cb2 receptor antagonists (sr141716a and sr144528, respectively) and nonselective cb1/cb2 cannabinoid receptor agonists (cp55,940 or win 55212-2) on macropha ... | 2000 | 10733093 |
| early events and implication of f-actin and annexin i associated structures in the phagocytic uptake of brucella suis by the j-774a.1 murine cell line and human monocytes. | brucella spp. are facultative, intracellular pathogenic bacteria that cause brucellosis, a zoonosis affecting mammalian species. brucella entry into myelomonocytic cell lines is highly enhanced by opsonization. few studies have been undertaken to unravel the first interactions between these bacteria and their host cells. this paper deals with early events following contact of brucella suis with the j-774a.1 phagocytic cell line and differentiated monocytes. phagocytic uptake of bacteria was docu ... | 2000 | 10839971 |
| complementation of brucella abortus rb51 with a functional wboa gene results in o-antigen synthesis and enhanced vaccine efficacy but no change in rough phenotype and attenuation. | brucella abortus rb51 is a stable rough, attenuated mutant vaccine strain derived from the virulent strain 2308. recently, we demonstrated that the wboa gene in rb51 is disrupted by an is711 element (r. vemulapalli, j. r. mcquiston, g. g. schurig, n. srirauganathan, s. m. halling, and s. m. boyle, clin. diagn. lab. immunol. 6:760-764, 1999). disruption of the wboa gene in smooth, virulent b. abortus, brucella melitensis, and brucella suis results in rough, attenuated mutants which fail to produc ... | 2000 | 10858205 |
| fluorescent whole-cell hybridization with 16s rrna-targeted oligonucleotide probes to identify brucella spp. by flow cytometry. | a whole-cell hybridization assay with fluorescent oligonucleotide probes derived from the 16s rrna sequence of brucella abortus in combination with flow cytometry has been developed. with the three fluorescent probes selected, a positive signal was observed with all the representative strains of the species and biovars of brucella and with a total of nine different brucella clinical isolates. using the b9 probe in the hybridization assay, it was possible to discriminate between brucella suis bio ... | 2000 | 10878084 |
| functional analysis of the clpatpase clpa of brucella suis, and persistence of a knockout mutant in balb/c mice. | the protein clpa belongs to a diverse group of polypeptides named clpatpases, which are highly conserved, and which include several molecular chaperones. in this study the gene encoding the 91 kda protein b-clpa of the facultative intracellular pathogen brucella suis, which showed 70% identity to clpa of rhodobacter blasticus, was identified and sequenced. following heterologous expression in escherichia coli strains sg1126 (deltaclpa) and sg1127 (deltalon deltaclpa), b-clpa replaced the functio ... | 2000 | 10878125 |
| activation of human vgamma9vdelta2 t cells by a brucella suis non-peptidic fraction impairs bacterial intracellular multiplication in monocytic infected cells. | human gamma delta t cells are considered to play an important role in the early response to infection with intracellular pathogens. evidence has been presented that the percentage of gamma delta t cells with vgamma9vdelta2 phenotype is dramatically increased in the peripheral blood of patients with acute brucellosis. this specific gd t-cell subpopulation is known to be activated by small non-peptidic molecules that can either be produced by the pathogen itself or released from damaged cells afte ... | 2000 | 10886403 |
| evaluation of primary binding assays for presumptive serodiagnosis of swine brucellosis in argentina. | an indirect enzyme-linked immunosorbent assay (ielisa), a competitive elisa (celisa), and a fluorescence polarization assay (fpa) for the presumptive serological diagnosis of swine brucellosis were evaluated using two populations of swine sera: sera from brucellosis-free canadian herds and sera from argentina selected based on positive reactions in the buffered antigen plate agglutination test (bpat) and the 2-mercaptoethanol (2-me) test. in addition, sera from adult swine from which brucella su ... | 2000 | 10973463 |
| brucella suis-impaired specific recognition of phagosomes by lysosomes due to phagosomal membrane modifications. | brucella species are gram-negative, facultatively intracellular bacteria that infect humans and animals. these organisms can survive and replicate within a membrane-bound compartment in phagocytic and nonprofessional phagocytic cells. inhibition of phagosome-lysosome fusion has been proposed as a mechanism for intracellular survival in both types of cells. however, the biochemical mechanisms and microbial factors implicated in brucella maturation are still completely unknown. we developed two di ... | 2001 | 11119541 |
| aromatic compound-dependent brucella suis is attenuated in both cultured cells and mouse models. | the aroc gene of the facultative intracellular pathogen brucella suis was cloned and sequenced. the cloned aroc gene complements escherichia coli and salmonella enterica serovar typhimurium aroc mutants. a b. suis aroc mutant was found to be unable to grow in a defined medium without aromatic compounds. the mutant was highly attenuated in tissue culture (thp1 macrophages and hela cells) and murine virulence models. | 2001 | 11119550 |
| v gamma 9v delta 2 t cells impair intracellular multiplication of brucella suis in autologous monocytes through soluble factor release and contact-dependent cytotoxic effect. | human vgamma9vdelta2 t cells are considered to play an important role in brucellosis, as this population is dramatically increased in peripheral blood of patients during the acute phase of the infection. this t lymphocyte population has been largely demonstrated to be activated by small m.w. nonpeptidic molecules from natural or synthetic origin. we recently identified a nonpeptidic fraction of brucella suis that specifically activates human vgamma9vdelta2 t cells. using a two-separate-chambers ... | 2000 | 11120844 |
| identification of the nik gene cluster of brucella suis: regulation and contribution to urease activity. | analysis of a brucella suis 1330 gene fused to a gfp reporter, and identified as being induced in j774 murine macrophage-like cells, allowed the isolation of a gene homologous to nika, the first gene of the escherichia coli operon encoding the specific transport system for nickel. dna sequence analysis of the corresponding b. suis nik locus showed that it was highly similar to that of e. coli except for localization of the nikr regulatory gene, which lies upstream from the structural nikabcde ge ... | 2001 | 11133934 |
| limited genetic diversity of brucella spp. | multilocus enzyme electrophoresis (mlee) of 99 brucella isolates, including the type strains from all recognized species, revealed a very limited genetic diversity and supports the proposal of a monospecific genus. in mlee-derived dendrograms, brucella abortus and a marine brucella sp. grouped into a single electrophoretic type related to brucella neotomae and brucella ovis. brucella suis and brucella canis formed another cluster linked to brucella melitensis and related to rhizobium tropici. th ... | 2001 | 11136777 |
| real-time detection of brucella abortus, brucella melitensis and brucella suis. | real-time pcr-based assays specific for brucella abortus, brucella melitensis and brucella suis were developed. the assays utilize an upstream primer that is derived from 3' end of the genetic element is 711, whereas the downstream primers and probes are designed from signature sequences specific to a species or a biovar. the pcr reactions were monitored for fluorescence resonance energy transfer by including two adjacent labeled probes that hybridize to the amplicons as they are formed. the ups ... | 2001 | 11162079 |
| secretion of listeriolysin by brucella suis inhibits its intramacrophagic replication. | the introduction into brucella suis 1330 of a plasmid allowing the heterologous expression of a hybrid cytolysin containing listeriolysin from listeria monocytogenes, and its export via the escherichia coli hemolysin secretion pathway, resulted in secretion of active listeriolysin monitored by erythrocyte lysis. in contrast to observations with the nonhemolytic control strain, the phagosomes of infected human monocytes containing the hemolytic b. suis were partially disrupted, and this strain fa ... | 2001 | 11254648 |
| evaluation of the fluorescence polarization assay and comparison to other serological assays for detection of brucellosis in cervids. | the complement fixation test (cft), competitive enzyme immunoassay (celisa), indirect enzyme immunoassay (ielisa) and fluorescence polarization assay (fpa) were evaluated for the detection of antibodies to brucella abortus and brucella suis biotype 4 in caribou (rangifer tarandus caribou), elk (cervus elapus), red deer (cervus elapus), and reindeer (rangifer tarandus tarandus). when combining the data the fpa and the celisa were determined to be the most suitable tests for serodiagnosis of cervi ... | 2001 | 11272484 |
| characterization of brucella suis clpb and clpab mutants and participation of the genes in stress responses. | pathogens often encounter stressful conditions inside their hosts. in the attempt to characterize the stress response in brucella suis, a gene highly homologous to escherichia coli clpb was isolated from brucella suis, and the deduced amino acid sequence showed features typical of the clpb atpase family of stress response proteins. under high-temperature stress conditions, clpb of b. suis was induced, and an isogenic b. suis clpb mutant showed increased sensitivity to high temperature, but also ... | 2001 | 11274130 |
| visceral abscesses due to brucella suis infection in a retired pig farmer. | a 78-year-old retired pig farmer developed brucellosis and died of liver failure >20 years after her last exposure to infected livestock. this is an exceptionally long incubation period for this infection, which usually presents within weeks of exposure. | 2001 | 11283821 |
| major outer membrane protein omp25 of brucella suis is involved in inhibition of tumor necrosis factor alpha production during infection of human macrophages. | brucella spp. can establish themselves and cause disease in humans and animals. the mechanisms by which brucella spp. evade the antibacterial defenses of their host, however, remain largely unknown. we have previously reported that live brucellae failed to induce tumor necrosis factor alpha (tnf-alpha) production upon human macrophage infection. this inhibition is associated with a nonidentified protein that is released into culture medium. outer membrane proteins (omps) of gram-negative bacteri ... | 2001 | 11447156 |
| molecular, antigenic, and functional analyses of omp2b porin size variants of brucella spp. | omp2a and omp2b are highly homologous porins present in the outer membrane of the bacteria from the genus brucella, a facultative intracellular pathogen. the genes coding for these proteins are closely linked in the brucella genome and oriented in opposite directions. in this work, we present the cloning, purification, and characterization of four omp2b size variants found in various brucella species, and we compare their antigenic and functional properties to the omp2a and omp2b porins of bruce ... | 2001 | 11466287 |
| the brucella suis homologue of the agrobacterium tumefaciens chromosomal virulence operon chve is essential for sugar utilization but not for survival in macrophages. | brucella strains possess an operon encoding type iv secretion machinery very similar to that coded by the agrobacterium tumefaciens virb operon. here we describe cloning of the brucella suis homologue of the chve-ggua-ggub operon of a. tumefaciens and characterize the sugar binding protein chve (78% identity), which in a. tumefaciens is involved in virulence gene expression. b. suis chve is upstream of the putative sugar transporter-encoding genes ggua and ggub, also present in a. tumefaciens, b ... | 2001 | 11514518 |
| a putative type iii secretion gene cluster is widely distributed in the burkholderia cepacia complex but absent from genomovar i. | using probes constructed from ralstonia solanacearum and burkholderia pseudomallei, putative type iii secretion (tts) genes were identified in burkholderia cepacia j2315 (genomovar iii). a cosmid clone containing dna with homology to five tts genes was sub-cloned and regions were sequenced in order to design oligonucleotides for polymerase chain reaction assays. these indicated that two putative tts genes (bcscq and bcscv) were present in all members of the b. cepacia complex with the exception ... | 2001 | 11557147 |
| actinobacillus actinomycetemcomitans harbours type iv secretion system genes on a plasmid and in the chromosome. | nine contiguous genes encoding a potential type iv secretion system have been identified in the chromosome of actinobacillus actinomycetemcomitans strain vt747 and on a plasmid (pvt745) in strain vt745. seven of these genes encode predicted proteins that share significant homology with type iv secretion proteins in bordetella pertussis (ptl operon), brucella melitensis biovar suis and agrobacterium tumefaciens (virb operons), where they are involved in protein secretion, pathogen intracellular s ... | 2001 | 11700353 |
| development of a brucella suis specific hybridisation probe and pcr which distinguishes b. suis from brucella abortus. | a genomic library was prepared from brucella suis dna (mboi digested) and cloned into the bamhi site of puc18. colony hybridisation using a probe prepared from purified b. suis dna labelled with alpha 32p was carried out to identify colonies of interest. about 20 colonies, which gave an intense signal upon hybridisation with whole b. suis genomic dna as a probe, were selected. because of the high degree of dna homology between b. suis and brucella abortus, a short probe was chosen as it would mo ... | 2002 | 11731177 |
| osteoarticular brucellosis with long latent period. | we describe the case of a young male who manifested brucella suis osteomyelitis after a 5-year period of relapsing monoarthritis. we discuss some of the difficulties regarding the diagnosis of osteoarticular brucellosis and review presentation patterns of the disease. | 2001 | 11771533 |
| the brucella suis virb operon is induced intracellularly in macrophages. | a type iv secretion system similar to the virb system of the phytopathogen agrobacterium tumefaciens is essential for the intracellular survival and multiplication of the mammalian pathogen brucella. reverse transcriptase-pcr showed that the 12 genes encoding the brucella suis virb system form an operon. semiquantitative measurements of virb mrna levels by slot blotting showed that transcription of the virb operon, but not the flanking genes, is regulated by environmental factors in vitro. flow ... | 2002 | 11830669 |
| induction of dnak through its native heat shock promoter is necessary for intramacrophagic replication of brucella suis. | the heat shock protein dnak is essential for intramacrophagic replication of brucella suis. the replacement of the stress-inducible, native dnak promoter of b. suis by the promoter of the constitutively expressed bla gene resulted in temperature-independent synthesis of dnak. in contrast to a dnak null mutant, this strain grew at 37 degrees c, with a thermal cutoff at 39 degrees c. however, the constitutive dnak mutant, which showed high sensitivity to h(2)o(2)-mediated stress, failed to multipl ... | 2002 | 11854256 |
| role of cholesterol and the ganglioside gm(1) in entry and short-term survival of brucella suis in murine macrophages. | brucella species are gram-negative, facultative intracellular bacteria that infect humans and animals. these organisms can survive and replicate within a membrane-bound compartment inside professional and nonprofessional phagocytic cells. inhibition of phagosome-lysosome fusion has been proposed as a mechanism for intracellular survival in both types of cells. we have previously shown that the maturation inhibition of the brucella-containing phagosome appears to be restricted at the phagosomal m ... | 2002 | 11854258 |
| bacterial secrets of secretion: euroconference on the biology of type iv secretion processes. | type iv secretion systems (tfss) mediate secretion or direct cell-to-cell transfer of virulence factors (proteins or protein-dna complexes) from many gram-negative animal, human and plant pathogens, such as agrobacterium tumefaciens, bartonella tribocorum, bordetella pertussis, brucella suis, helicobacter pylori, legionella pneumophila and rickettsia prowazekii, into eukaryotic cells. bacterial conjugation is also classified as a tfss-like process mediating the spread of broad-host-range plasmid ... | 2002 | 11918819 |
| identification of a quorum-sensing signal molecule in the facultative intracellular pathogen brucella melitensis. | brucella melitensis is a gram-negative alpha2-proteobacterium responsible for abortion in goats and for malta fever in humans. this facultative intracellular pathogen invades and survives within both professional and nonprofessional phagocytes. a dichloromethane extract of spent culture supernatant from b. melitensis induces bioluminescence in an escherichia coli acyl-homoserine lactone (acyl-hsl) biosensor strain based upon the activity of the lasr protein of pseudomonas aeruginosa. hplc fracti ... | 2002 | 12010991 |
| lipopolysaccharide heterogeneity in brucella strains isolated from marine mammals. | smooth lipopolysaccharides (s-lpss) from brucella strains isolated from seals, dolphins, porpoises, an otter and a minke whale were characterized by elisa using monoclonal antibodies (mabs) directed against seven previously defined o-polysaccharide (o-ps) epitopes and by western blot after sds-page. all strains studied were a-dominant as shown by specific polyclonal sera and this was also confirmed by the mabs. however, binding patterns in elisa of mabs to the specific common (c) epitopes were r ... | 2002 | 12160318 |
| increased prevalence of brucella suis and pseudorabies virus antibodies in adults of an isolated feral swine population in coastal south carolina. | two hundred twenty seven adult (> 8 mo) feral swine (sus scrofa) trapped from april through july 1999 at three locations on a coastal south carolina (usa) peninsula with restricted ingress and egress were tested for brucella suis and pseudorabies virus (prv) antibodies. approximately 44% of the animals tested positive for b. suis antibodies and 61% tested positive for antibodies to prv. previous surveys (1976 and 1992) of feral swine at the same location with similar methods indicated lower sero ... | 2002 | 12238392 |
| the brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts. | the 3.31-mb genome sequence of the intracellular pathogen and potential bioterrorism agent, brucella suis, was determined. comparison of b. suis with brucella melitensis has defined a finite set of differences that could be responsible for the differences in virulence and host preference between these organisms, and indicates that phage have played a significant role in their divergence. analysis of the b. suis genome reveals transport and metabolic capabilities akin to soil/plant-associated bac ... | 2002 | 12271122 |
| brucellosis in central america. | brucellosis is a disease of domestic animals and humans in central america (ca). bovine and swine brucellosis caused by brucella abortus and brucella suis, respectively, have been identified in all ca countries, while ovine and caprine brucellosis caused by brucella melitensis has been detected in guatemala. the prevalence of bovine brucellosis is estimated between 4 and 8%, with higher prevalence in dairy herds, with losses calculated at 25 million us dollars per year. national control programs ... | 2002 | 12414131 |
| brucellosis in the european union and norway at the turn of the twenty-first century. | control and eradication programs of brucellosis in cattle, sheep, goats and pigs have been more or less successfully implemented within the member states (ms) of the european union (eu) and norway after word war ii. as a result, the epidemiological situation of animal brucellosis is extremely diverse among different ms or regions within a ms and among the different animal species. some ms, mainly north european countries, and norway are declared "officially bovine brucellosis free" and/or "offic ... | 2002 | 12414139 |
| the intramacrophagic environment of brucella suis and bacterial response. | phagocytes have developed various antimicrobial defense mechanisms to eliminate pathogens. they comprise the oxidative burst, acidification of phagosomes, or fusion of phagosomes with lysosomes. facultative intracellular bacteria, in return, have developed strategies counteracting the host cell defense, resulting in intramacrophagic survival. until lately, only very little was known about the phagosomal compartment containing brucella spp., the environmental conditions the bacteria encounter, an ... | 2002 | 12414150 |
| the innate immune response against brucella in humans. | pathogens have developed different strategies to survive and multiply within their host. among them is the ability to control phagocyte apoptosis while another is to affect the expression of cytokines which is necessary for a normal protective function of the immune response. to establish themselves and cause chronic disease in humans and animals, brucella spp. invade and proliferate within monocytic phagocytes. we have established that in humans, brucella suis impairs the apoptosis of monocytes ... | 2002 | 12414158 |
| how to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. | collaborative work was financed by the eu to develop and assess new diagnostic tools that can differentiate between bovine brucellosis and bovine infections due to yersinia enterocolitica o:9 either in conjunction with, or as an alternative to, the classical serological, bacteriological or allergic skin tests. sixteen heifers were experimentally infected with brucella abortus biovar 1 (five heifers), brucella suis biovar 2 (two heifers), y. enterocolitica o:9 (six heifers) and y. enterocolitica ... | 2002 | 12414165 |
| brucella vaccines in wildlife. | brucellosis has been known to exist in populations of wildlife since the early part of the 20th century. at the beginning of this century in the us, brucella abortus is a problem in elk and bison in the greater yellowstone area, b. suis is prevalent in millions of feral swine in most of the southern states, and caribou/reindeer in alaska are infected with b. suis biovar 4. brucellosis has been virtually eliminated in domestic livestock in the us after decades of expensive governmental disease pr ... | 2002 | 12414169 |
| clinical and diagnostic aspects of relapsing meningoencephalitis due to brucella suis. | 2002 | 12415479 | |
| the analysis of the intramacrophagic virulome of brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. | the pathogen brucella suis resides and multiplies within a phagocytic vacuole of its host cell, the macrophage. the resulting complex relationship has been investigated by the analysis of the set of genes required for virulence, which we call intramacrophagic virulome. ten thousand two hundred and seventy-two minitn5 mutants of b. suis constitutively expressing gfp were screened by fluorescence microscopy for lack of intracellular multiplication in human macrophages. one hundred thirty-one such ... | 2002 | 12438693 |
| production of the type iv secretion system differs among brucella species as revealed with virb5- and virb8-specific antisera. | expression of the virb operon, encoding the type iv secretion system required for brucella suis virulence, occurred in the acidic phagocytic vacuoles of macrophages and could be induced in minimal medium at acidic ph values. to analyze the production of virb proteins, polyclonal antisera against b. suis virb5 and virb8 were generated. western blot analysis revealed that virb5 and virb8 were detected after 3 h in acidic minimal medium and that the amounts increased after prolonged incubation. unl ... | 2003 | 12595417 |
| role of the brucella suis lipopolysaccharide o antigen in phagosomal genesis and in inhibition of phagosome-lysosome fusion in murine macrophages. | brucella species are gram-negative, facultative intracellular bacteria that infect humans and animals. these organisms can survive and replicate within a membrane-bound compartment inside professional and nonprofessional phagocytic cells. inhibition of phagosome-lysosome fusion has been proposed as a mechanism for intracellular survival in both cell types. however, the molecular mechanisms and the microbial factors involved are poorly understood. smooth lipopolysaccharide (lps) of brucella has b ... | 2003 | 12595466 |
| [anti-lysozyme activity of bacteria of the genus brucella]. | the antilysozyme activity of 122 brucella strains has been studied. the antilysozyme sign has been found to be widely spread among different brucella species. the antilysozyme sign has proved to be most pronounced in b. melitensis. | 2000 | 12712527 |