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studies of the subgingival microflora in patients with acquired immunodeficiency syndrome.two unique forms of periodontal disease, hiv-gingivitis and hiv-periodontitis, have been described in patients with acquired immunodeficiency syndrome (aids). in order to determine the bacterial species associated with periodontitis in aids patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 aids patients with periodontitis. the presence of putative periodontal pathogens including actinobacillus actinomycetemcomitans, bacteroides intermedius, por ...19902123926
monoclonal antibody-coated latex agglutination assay for identification of actinobacillus actinomycetemcomitans.three monoclonal antibodies (mabs) to lipopolysaccharide of actinobacillus actinomycetemcomitans strain y4 (serotype b) and eight mabs to a serotype b-specific polysaccharide antigen of strain y4 were obtained. latex particles sensitized with an mab to the y4 lipopolysaccharide produced a positive agglutination with whole cells of all three serotypes of a. actinomycetemcomitans, but not with haemophilus aphrophilus, haemophilus paraphrophilus, haemophilus influenzae, porphyromonas (bacteroides) ...19902124492
in vitro survival of bacteroides intermedius in five transport media, alone or in the presence of streptococcus sanguis.the survival rate of bacteroides intermedius was first tested in monoculture, and streptococcus sanguis was then added in 5 different transport media; 2 nonnutritious media, the viability-preserving medium of the university of göteborg no. iv (vmg iv), reduced transport fluid (rtf), and 3 nutritious media, thioglycolate medium (tg), peptone yeast extract medium (py) and py medium with 1% glucose (pyg). all manipulations were carried out in an anaerobic chamber. after a given transport time (6 or ...19902129136
demonstration of bacteroides intermedius in periapical tissue using indirect immunofluorescence microscopy.the presence of bacteria in periapical lesions of teeth with necrotic pulp has been demonstrated by microbiological sampling of periapical lesions during endodontic surgery. the purpose of this study was to confirm the presence of bacteroides intermedius in a periapical granuloma using an indirect immunofluorescence technique. histologic sections of a periapical granuloma were incubated with a rabbit antiserum to b. intermedius. after incubation with secondary antibody (fluorescein-conjugated go ...19902133307
[mitogenic activity of lipopolysaccharide from bacteroides intermedius against c3h/hej mice spleen cells].bacteroides intermedius is one of the periodontal disease generative bacteria. lipopolysaccharide preparation obtained from b. intermedius atcc 25611 and atcc 33563 strains was investigated as to limulus activity observed with pyrodic (endotoxin specific) reagent, and mitogenic activity in both normal and tolerant mice induced by escherichia coli. limulus activities of all lps's were nearly similar to that of e. coli. lps's preparation from b. intermedius were found to be strongly mitogenic for ...19902134944
[changes of factors on disease activity in advancing periodontitis].two test teeth, anteriors with greater than or equal to 6 mm deep periodontal pockets from each of 10 patients with advancing periodontitis were included in this study. the clinical signs of advancing periodontitis, generalized moderate to deep pockets and to severe loss of alveolar bone, were observed in young adult. there have been several reports on factors, which reflect the conversion clinically from infection by highly pathogenic plaque bacteria to a from of periodontitis displaying relati ...19902135609
prepubertal periodontitis: a review of diagnostic criteria, pathogenesis, and differential diagnosis.this paper reviews the literature on prepubertal periodontitis (pp) according to the definition and the classification of pp by page. the generalized form of pp (g-pp) defined by page was identified as the oral manifestation of a systemic disease called leukocyte adhesion deficiency (lad) and thus the pathogenesis is known at the molecular level. on the other hand, the localized form of pp (l-pp) is a disease defined by clinical criteria and its pathogenesis is not known at the molecular level. ...19902137170
selective modulation of bacterial attachment to oral epithelial cells by enzyme activities associated with poor oral hygiene.the present investigation explored the hypothesis that elevated levels of certain enzymes in the gingival crevicular environment of individuals with poor oral hygiene and/or gingival inflammation may modify the surfaces of epithelial cells and thereby modulate the types of bacteria which attach and colonize. buccal epithelial cells treated with neuraminidase and certain proteases were used as a model for study. bacteria studied included streptococcus sanguis and streptococcus mitis which have be ...19902141877
inhibition of protease activities of periodontopathic bacteria by extracts of plants used in kenya as chewing sticks (mswaki).extracts from five plants used as chewing sticks, and tannic acid, gallic acid and methyl ester of gallic acid, were tested for their ability to inhibit proteolytic activities of three strains of bacteroides gingivalis, three strains of bacteroides intermedius and two strains of treponema denticola. aqueous extract from the plants rhus natalensis and euclea divinorum were the most inhibitory of those tested, inhibiting by 50% the proteolytic activity of the test organisms, at concentrations of u ...19902142592
alterations in cell morphology and cytoskeletal proteins in gingival fibroblasts exposed to a bacteroides gingivalis extract.a soluble sonic extract (sse) from bacteroides gingivalis caused a dose-dependent inhibition of gingival fibroblast growth, reduced cell attachment and altered cell morphology. most of its cytotoxic activity was destroyed by heating, indicating that the factor(s) was a protein rather than endotoxin. cells, grown in the presence of, or on, root surfaces pretreated with 100-200 micrograms sse/ml, partially retracted from the substratum and exhibited extensive surface blebbing and finger-like protr ...19902148946
suppression of bactericidal activity of human polymorphonuclear leukocytes by bacteroides gingivalis.the direct effects of the culture supernatant of oral microorganisms on the bactericidal activity of human polymorphonuclear leukocytes (pmns) were investigated. the bactericidal activity of pmns, which were preincubated with the supernatant of bacteroides gingivalis, bacteroides intermedius, bacteroides melaninogenicus or phosphate-buffered saline, was examined by counting the surviving bacteria. b. gingivalis-treated pmns were found to have a diminished ability for killing bacteria in the pres ...19902153632
effect of ph on the growth and proteolytic activity of porphyromonas gingivalis and bacteroides intermedius.the effect of ph on the growth and proteolytic activity of the type strain and fresh isolates of porphyromonas gingivalis and bacteroides intermedius was investigated. b. intermedius strains grew with and without glucose at a ph as low as 5.0. these bacteria grew almost as well as streptococcus mutans at ph 5.0 and better than actinomyces viscosus at ph 5.5 and 5.0. some b. intermedius strains raised the culture ph when grown at a low ph without glucose. in contrast, p. gingivalis strains grew o ...19902191980
bacteroides lipopolysaccharides (lps) induce anaphylactoid and lethal reactions in lps-responsive and -nonresponsive mice primed with muramyl dipeptide.lipopolysaccharides (lps) (100 micrograms/mouse) from bacteroides gingivalis elicited anaphylactoid reactions in n-acetylmuramyl-l-alanyl-d-isoglutamine (mdp) (100 micrograms)-primed c3h/hen mice 0-48 h after mdp injection. the reaction resulted in death within 1 h when the lps was injected 2-10 h after the mdp injection. lps prepared from other bacteroides and salmonella species but not from others, including escherichia coli, could induce the reaction. lps-nonresponsive c3h/hej and c57bl/6 and ...19902197336
application of monoclonal antibodies to the detection of black-pigmented bacteroides spp. in subgingival plaques by immunoslot blot assay.the aim of the present study was to assess the application of monoclonal antibodies to the detection of black-pigmented bacteroides spp. in subgingival plaques by immunoslot blot assay. subgingival plaque samples from adult periodontal patients were examined by immunoslot blot assay with monoclonal antibodies that specifically recognize bacteroides gingivalis, bacteroides intermedius serogroups i and ii, and bacteroides melaninogenicus. the assay can detect specifically these bacteroides spp. in ...19902229348
biochemical and serological characterization of bacteroides intermedius strains isolated from the deep periodontal pocket.fifty-one fluorescence-positive black-pigmented bacteroides strains obtained from 51 patients with deep periodontal pockets (greater than 6 mm) were identified and characterized. fifty of these strains were presumptively identified as bacteroides intermedius according to the indole reaction. this was confirmed by further biochemical characterization. the 50 strains from diseased sites were then compared with 16 b. intermedius strains isolated from periodontally healthy individuals with no signs ...19902229351
study of the antigenic relationships between strains of bacteroides, intermedius, b. melaninogenicus, b. corporis, and b. denticola revealed by immunoblotting with rabbit antisera.antigen profiles of saccharolytic oral black-pigmented bacteroides have been developed by western blotting. visual comparisons indicated extensive cross-reactions between b. intermedius, b. melaninogenicus, b. denticola, and b. corporis. porphyromonas gingivalis, p. asaccharolyticus, and b. buccae showed less cross-reaction. quantitation of antigenic similarity was made from densitometric scans. calculation of the jackard coefficient gave results of 33-72% similarity among the saccharolytic pigm ...19902249172
actinobacillus actinomycetemcomitans and bacteroides intermedius in human periodontitis: age relationship and mutual association.this study examined age relationships and mutual interrelationships between cultivable actinobacillus actinomycetemcomitans and bacteroides intermedius in 1624 periodontitis patients, 15 to 89 years of age. each subject contributed a pooled subgingival sample, obtained from 3 deep periodontal pockets with paper points. a. actinomycetemcomitans occurred with higher prevalence (74%) and mean recovery (7% in culture-positive patients) in patients less than 25 years old than in adult and geriatric p ...19902250080
cytotoxic and immunostimulatory effects of bacteroides cell products.the etiologic role of bacteroides in both periodontal and periapical infections has been well documented, with current interest focusing on the specific pathogenic mechanisms involved. the effects of cell fractions derived from bacteroides gingivalis (bg), bacteroides intermedius (bi), and bacteroides asaccharolyticus (ba) have been studied in vitro through: an assessment of the direct cytotoxic effects on human gingival fibroblasts using a tetrazolium dye reduction assay, an evaluation of murin ...19902250226
the effect of clindamycin on the microbiota associated with refractory periodontitis.the purpose of this investigation was to determine the effect of clindamycin hydrochloride, as an adjunct to scaling, on the microbiota associated with refractory periodontitis and to elucidate the probable causative bacteria associated with the disease. microbial samples were collected from a subset of 9 patients with severe adult periodontitis who had not responded to conventional treatment modalities including the use of tetracycline and other antibiotics. microbial samples were collected fro ...19902254836
[a comparative evaluation of oral and parenteral cephalosporins in inhibiting the growth of bacteria pathogenic to the periodontium].a study was carried out in vitro to assess the inhibitory activity on the growth of bacteroides gingivalis and bacteroides intermedius using two oral cephalosporins (cephalexin and cephradine) in comparison to two parenteral cephalosporins (cefotaxime and ceftriaxon) and the classic tetracycline hcl. the results of the study show that the inhibitory activity on the growth of the two periodontopathogenic bacteria produced by the oral cephalosporins is comparable to that of other antibiotics studi ...19902287333
detection of conjugal transfer systems in oral, black-pigmented bacteroides spp.oral, black-pigmented bacteroides spp. are important pathogens in oral anaerobic infections and dental disease. we detected conjugation systems in isolates of bacteroides denticola and bacteroides intermedius that transferred tetracycline resistance (tetr) and penicillin resistance to bacteroides buccae and to bacteroides fragilis, an intestinal bacteroides species. a cloned tetr gene from b. fragilis hybridized to the transferable tetr locus in the oral strains, indicating that genetic exchange ...19902294095
suspected periodontopathogens in erupting third molar sites of periodontally healthy individuals.29 periodontally healthy subjects (11 female and 18 male) with a mean age of 24 years (range 19 to 38 years) and with partially erupted lower third molars participated in this study. 18 subjects demonstrated no signs or symptoms of acute inflammation and were without pain (group a). 5 subjects showed redness of the pericoronal tissues and experienced pain upon palpation (group b). 6 subjects suffered from acute pain and exhibited formation of pus (group c). microbiological samples were taken fro ...19902295708
growth stimulation of treponema denticola by periodontal microorganisms.previous experiments have indicated that enrichment of subgingival plaque in human serum can lead to the accumulation of treponema denticola. t. denticola depends on bacterial interactions for its growth in serum. aim of the present study was to identify specific microorganisms involved in the growth stimulation of t. denticola. to this end, strains isolated from previous plaque enrichment cultures were tested for growth stimulation in co-cultures with t. denticola. in addition, growth of t. den ...19902321929
assessing periodontal pathogens in children with varying levels of oral hygiene.this study investigated the presence of organisms actinobacillus actinomycetecomitans (a.a.), bacteroides intermedius (b.i.), and bacteroides gingivalis (b.g.), believed to be associated with periodontal disease etiology in children. the study included fifty children, aged six to fourteen years, selected from patients seeking routine dental care at children's hospital national medical center. a modified plaque index (mpi) denoting the clinical presence and severity of the periodontal disease was ...19902345212
[the in vitro sensitivity of bacteroides gingivalis and bacteroides intermedius to different classes of antibiotic chemical molecules].a study was carried out to assess the in vitro sensitivity of bacteroides gingivalis and bacteroides intermedius to various classes of chemio-antibiotic molecules. the activities of two cephalosporins (cefuroxime and cefotaxime), which have never previously been used for periodontal therapy, were also evaluated. results showed that the cephalosporins possessed an equal or greater inhibitory activity against the bacteria studied than traditional tetracyclines, and a greater inhibitory activity th ...19902381396
antimicrobial effect of chlorhexidine in a controlled release delivery system.chlorhexidine is widely used as a mouth rinse in the prevention and treatment of periodontal diseases and dental caries. the purpose of the present study was to evaluate the in vitro antimicrobial effect of chlorhexidine in a controlled release delivery system. the controlled release dispenser comprised a polymeric inner core matrix containing the medicament with an outer vinyl membrane controlling the drug release. the effect on the following bacteria was studied: actinobacillus actinomycetemco ...19902390965
clinical and microbiologic findings that may contribute to dental implant failure.clinical and dna probe analysis were used to evaluate 36 failing implant sites in 13 patients. failing implants showed evidence of increased mobility and a high incidence of peri-implant radiolucencies in radiographs. the probing depth was greater than 6 mm in 58% of the sites measured. moderate levels of actinobacillus actinomycetemcomitans, bacteroides intermedius, and bacteroides gingivalis were detected with dna probe analysis.19902391137
occurrence of bacteroides gingivalis, bacteroides intermedius and actinobacillus actinomycetemcomitans in severe periodontitis in relation to age and treatment history.a total of 242 subjects including 138 untreated severe periodontitis patients and 104 patients with refractory periodontal disease, previously treated for severe periodontitis, were examined for the occurrence of actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. pooled subgingival samples of representative periodontal lesions were used for anaerobic cultivation on blood agar and for the enumeration of a. actinomycetemcomitans on selective tsbv medium. 97% ...19902398137
bacteroides gingivalis and bacteroides intermedius recognize different sites on human fibrinogen.bacteroides (porphyromonas) gingivalis and bacteroides (porphyromonas) intermedius have been implicated in the etiology of human periodontal diseases. these organisms are able to bind and degrade human fibrinogen, and these interactions may play a role in the pathogenesis of periodontal disease. in attempts to map the bacterial binding sites along the fibrinogen molecule, we have found that strains of b. gingivalis and b. intermedius, respectively, recognize spatially distant and distinct sites ...19902404954
reproducibility of microbiological samples from periodontal pockets.duplicate microbiological samples, were taken 1 week apart using the paper point technique from a total of 112 untreated periodontal pockets greater than 6 mm deep in 16 adult periodontal patients. duplicate samples were also obtained from these sites 6 months following a therapy of oral hygiene instruction and supra- and subgingival debridement. the reproducibility of the total viable counts and the reproducibility of the proportions of various groups or species of microorganisms were studied f ...19902406295
production of broad-spectrum bacteriocin-like activity by group a streptococci of particular m-types.application of a bacteriocin production (p)-typing scheme to group a streptococci has shown that approximately 10% of the tested strains inhibit the growth of all 9 indicator bacteria, an activity referred to as p-type 777. production of such activity was found to be restricted to 14 m-serotypes and within these m-types the incidence of p-type 777 activity was very high. there was no evidence of any correlation with the t-antigenic composition of the bacteria. investigations of the conditions fo ...19852409712
human serum antibodies against bacteroides intermedius. antigenic heterogeneity impairs the interpretation of the host response. 19852416900
fluorescent antibody and flagella stains for rapid detection of bacteria at periodontally healthy and diseased sites.two rapid methods of identifying bacteria associated with periodontal disease were investigated to determine their diagnostic usefulness in longitudinal or epidemiologic studies. three nonmotile organisms were identified by fluorescent antibody stains (fa) while percentages of motile bacteria were assessed by counting all spirochetes, flagellated and nonflagellated organisms stained with a simplified silver-plating stain for flagella. relationships between disease activity and these bacteria fro ...19862428966
keratinolytic activity of cutaneous and oral bacteria.a test was developed to measure the keratinolytic activity of cutaneous and oral bacteria. keratin, labeled with fluorescein isothiocyanate, was used in a phosphate buffer (ph 7.2) with 1 mm dithiothreitol. the degradation of keratin was estimated by measuring the fluorescence of the degradation products in the supernatant of the reaction mixtures in a luminescence spectrometer. several oral and cutaneous bacteria were investigated: bacteroides gingivalis, bacteroides intermedius, treponema dent ...19872434427
synthetic oligodeoxynucleotide probes for the rapid detection of bacteria associated with human periodontitis.analysis of the subgingival microflora has recently implicated actinobacillus (haemophilus) actinomycetemcomitans and several black bacteroides species in the aetiology of juvenile, adult and rapidly progressing periodontitis. rapid bacteriological diagnosis has been hampered by the slow growth and fastidious nature of these bacteria. to construct diagnostic probes, dideoxy sequencing of the 16s rrna molecules from a. (h.) actinomycetemcomitans, haemophilus aphrophilus, bacteroides gingivalis, b ...19882469776
ultrastructure, serogrouping and localization of surface antigens of bacteroides intermedius.the surface ultrastructure of 21 strains of bacteroides intermedius was investigated by electron microscopy. rat monoclonal antibodies (mabs) were used to define serogroups and to detect the location of surface antigens. all 21 isolates had capsules as demonstrated by the use of wet and dry indian ink stains. negative staining of whole cells with 1% (w/v) methylamine tungstate showed that all 21 isolates carried clumped peritrichous fibrils with strain dependent morphology, density and length (l ...19892480991
[purification and some properties of neuraminidase from the culture medium of bacteroides loescheii atcc 15930].the purpose of this study is to investigate the mechanism on enhanced hemagglutination caused by neuraminidase from bacteroides in an aspect of zeta-potential levels on erythrocytes and inhibitors on hemagglutination activity. the neuraminidase from the culture medium of bacteroides loescheii atcc 15930 was purified by ultrafiltration followed by deae-sephacel anion exchange chromatography, fast protein, polypeptide, polynucleotide, liquid chromatography using mono p column and finally high perf ...19892488923
[osseointegrated implants in clinical dentistry. follow up maintenance phase].the aim of this study is to determine the method of maintenance care for support the functional condition during long period. the present paper reports on the result of osseointegrated titanium fixture in four complete denture patients. the marginal soft tissue reactions were investigated at the 1st, 3rd, 6th, 12th and 18th month after prothetic restrations by clinical examination and microbiological observations. all abutments were surrounded by clinically in healthy gingiva, however most indiv ...19892489293
[bacteriological study of gingivitis in pubertal children. 2. subgingival microflora cultivable on the nonselective blood agar medium].the present study involved cultural and microscopic characterization of the subgingival microorganisms of pubertal children with gingivitis. samples were obtained from eight children 12-14 years of age. gram-positive organisms made up 56% of the isolates and included mainly actinomyces israelii, actinomyces naeslundii. gram-negative anaerobic rods constituted 25% of the isolates and bacteroides intermedius (9.7%) was most predominant species. the selective media permitted significantly lower rec ...19892489550
[biological characterization of bacteroides intermedius specific monoclonal antibody].one monoclonal antibody (bif6) directed against bacteroides intermedius group i was developed by hybridoma technology. in the present study, we used culture medium from bif6 antibody-producing cells to characterize the antibody. its reactivity against b. intermedius (group i, ii), b. gingivalis, b. corporis, b. melaninogenicus, b. loescheii, and b. asaccharolyticus was detected by the enzyme-linked immunosorbent assay. bif6 monoclonal antibody specifically reacted with b. intermedius group i, bu ...19892489667
[igg, iga and igm serum antibodies against lipopolysaccharide from bacteroides intermedius in periodontal health and disease].serum antibody titers to lipopolysaccharide from bacteroides intermedius (b. intermedius) and bacteroides gingivalis (b. gingivalis) were measured by enzyme-linked immunosorbent assay in serum samples from subjects with healthy gingiva and patients with periodontitis. the igg antibody titers to lps from b. gingivalis were significantly higher in the patients group, compared with healthy controls. no such difference was found between igg activity to lps from b. intermedius in the healthy and the ...19892489807
bactericidal concentrations of chlorhexidine-digluconate, amine fluoride gel and stannous fluoride gel for subgingival bacteria tested in serum at short contact times.in vitro inhibitory and bactericidal concentrations in serum of chlorhexidine-digluconate, amine fluoride gel, stannous fluoride gel, stannous fluoride, metronidazole and amoxicillin were determined against bacteroides gingivalis, bacteroides intermedius, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and capnocytophaga sputigena. the minimal inhibitory concentration was assessed by the agar dilution technique. the killing curves and minimal bactericidal concentration of the antim ...19892524581
immunoadjuvant effects of periodontitis-associated bacteria.sonic extract (se) from periodontopathic bacteria were studied for their effects on the immune response and adjuvanticity. the preparations of se from a. actinomycetemcomitans, b. intermedius and b. gingivalis were found to have strong immunomodulating activity against the antigen itself and also sheep erythrocytes (srbc) in in vivo experiments using c3h/hen mice. all the se enhanced the immune response to antigen and srbc at low concentration. a strong immuno suppressive effect, however, was fo ...19892533257
[gene cloning of an actinobacillus actinomycetemcomitans y4 antigen that reacts to peripheral blood sera in advanced destructive periodontitis patients].actinobacillus actinomycetemcomitans (a.a.) has been implicated in the etiology of juvenile periodontitis and also of advanced destructive periodontitis (adp). it has been reported that the levels of the igg antibody against the a.a. in the peripheral blood sera of the adp patients were often high as well as those against the bacteroides gingivalis. to clone the genes of antigen reactive to the sera of the adp patients, we constructed a phage library of the a.a. strain y4 dna in lambda l47, whic ...19892533606
[effects of endotoxins from periodontopathic bacteria on the collagen metabolism of cultured normal human gingival fibroblasts].the present study was performed to investigate the effects of endotoxins from periodontopathic bacteria on collagen metabolism. endotoxins were extracted from bacteroides gingivalis 381 and bacteroides intermedius atcc 25611 using the hot-phenol method. a commercially available endotoxin from escherichia coli 0111: b4 was also used as a control. human gingival fibroblasts (gin-1, atcc crl 1292) were maintained with dmem containing 10% fbs. when the fibroblasts became confluent they were exposed ...19892562264
proteolytic activity in black-pigmented bacteroides species.black-pigmented bacteroides species are frequently found in dentoalveolar abscesses. one general mechanism of bacterial virulence is the production of extracellular enzymes which degrade connective tissue or molecules associated with host defense. in this study the proteolytic activity of 18 bacterial strains from 9 black-pigmented bacteroides species was examined. bacteroides gingivalis degraded the greatest number of substrates studied and produced the highest levels of enzymatic activity. b. ...19892576979
dna probe detection of key periodontal pathogens in juveniles.recognition of juvenile forms of periodontitis have been shown to be directly linked with specific gram-negative rods, primarily actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. however, clinical application of these laboratory findings have generally been restricted to the research environment. the purpose of this study was to explore the relationship of these three pathogenic species in children using the reliable and accurate new technology of dna prob ...19892593067
effect of ultrasonic instrumentation on bacteroides intermedius.two physical mechanisms of ultrasound, cavitation and acoustic streaming, were investigated in an ultrasonic endodontic unit. in addition, the potential of ultrasonic instrumentation for disruption of bacteroides intermedius was examined at various time intervals. the ultrasonic file could not generate cavitation within the recommended power settings indicated for endodontic purposes. however, there was evidence of acoustic streaming. the latter phenomenon resulted in destruction of 21.6%, 30.4% ...19892598890
clinical and microbiological characteristics of treated periodontitis patients on maintenance care.a population of 98 adults previously treated for moderate to advanced adult periodontitis and currently maintained in a recall program for at least one year were recruited for this study. the ultimate purpose of the study is to determine whether the presence of actinobacillus actinomycetemcomitans, bacteroides gingivalis, and bacteroides intermedius in selected periodontal sites can predict the future clinical course of these patients, particularly with respect to disease recurrence. this report ...19892600753
prevalence of black-pigmented bacteroides species in root canal infections.the prevalence of black-pigmented bacteroides species in the root canals of 72 teeth with apical periodontitis was evaluated. twenty-two of the canals contained one or more species of black-pigmented bacteroides. bacteroides intermedius (14 strains) and bacteroides endodontalis (5 strains) were most common. of the species bacteroides gingivalis, bacteroides loeschei, and bacteroides denticola, 2, 3, and 1 strains, respectively, were isolated. the median number of bacterial cells recovered from t ...19892607261
antimicrobial susceptibilities of eubacterium, peptostreptococcus, and bacteroides isolated from root canals of teeth with periapical pathosis.eubacterium, peptostreptococcus, and bacteroides were isolated in high frequency from root canals with acute periapical inflammation. the antimicrobial susceptibilities of these strains were studied by determining minimum inhibitory concentrations of different agents. although all three kinds of isolates were susceptible to penicillins, the isolates other than black-pigmented bacteroides were less susceptible to cephems, tetracyclines, and macrorides with several resistant strains. all strains w ...19892607278
[toxicity of bacteroides intermedius surface extracts against mouse splenic lymphocyte].the purpose of the present study is to clarify in vitro toxicity of surface sonic extracts from bacteroides intermedius (b. intermedius) atcc25611, b. intermedius atcc 33563 and bacteroides gingivalis (b. gingivalis) 381 against mouse splenic lymphocyte. especially the surface extracts from b. intermedius atcc 25611 were fractionated by high performance liquid chromatography (hplc) on a column of deae. the following results were obtained: 1) the surface extracts from b. gingivalis 381 and b. int ...19892626166
the predominant cultivable microbiota of crevicular epithelial cells.the purpose of the present investigation was to compare the composition of the predominant cultivable microbiota associated with gingival crevicular epithelial cells with that of the unattached microbiota recovered from the same site. samples were taken from 2 diseased sites from 8 periodontal patients, by scraping the epithelial lining of the pocket with a curette. the epithelial cells were separated from the unattached subgingival bacteria by centrifugation in a reduced 50% percoll density gra ...19892628861
further studies on the degradation of immunoglobulins by black-pigmented bacteroides.the ability of several species of black-pigmented bacteroides to degrade immunoglobulins a and g was confirmed in this study. the cleavage products from igg strongly stimulated the growth of bacteria degrading igg. growth of bacteroides gingivalis on limiting media supplemented with igg paralleled growth on complete medium. the degradation of igg and iga by black-pigmented bacteroides appeared to occur in 2 stages. the molecules were broken into large fragments which were subsequently degraded i ...19892628862
immunomodulatory effects of bacteroides products on in vitro human lymphocyte functions.bacteroides spp. have been implicated in the pathogenesis of several diseases, including periodontal diseases. in this study sonic extracts of 6 bacteroides spp. were examined for their abilities to alter human lymphocyte function. we found that soluble extracts from bacteroides intermedius, bacteroides endodontalis, bacteroides asaccharolyticus, bacteroides melaninogenicus, and to a lesser degree bacteroides loescheii, caused dose-dependent inhibition of human lymphocyte responsiveness to both ...19892628864
[microbiological changes in subgingival flora after treatment with amoxicillin-clavulanic acid].the association amoxicillin-clavulanic acid can be employed as an alternative to the usual antibiotic therapy of periodontitis. the purpose of this study was to determine subgingival microbial changes in 33 patients with periodontitis after using amoxicillin (500 mgrs.-t.i.d.) and clavulanic acid (125 mgrs.-t.i.d.) for 5 days. it resulted clinically in a decrease of both gingival index and plaque index (not significant) and microbiologically in absence of the main bacterial pathogens found pretr ...19892638817
surface appendages, hemagglutination, and adherence to human epithelial cells of bacteroides intermedius.four types of morphologically distinct surface appendages were found on oral strains of bacteroides intermedius. these appendages, designated as a, b, c, and d, were different in size, with diameters of 1-2 nm, 12 nm, 8 nm, and 5 nm, respectively. twenty different strains were examined by electron microscopy and 5 strains, 5, 17, 27, 113, and 25611, were selected to be representative of the different appendages encountered. type a appendages were thin filaments peritrichously arranged on the cel ...19892640314
the occurrence of actinobacillus actinomycetemcomitans, bacteroides gingivalis, bacteroides intermedius and spirochaetes in the subgingival microflora in relation to the early onset of periodontitis in a group of adolescents.subgingival samples were obtained from mesial and distal surfaces of first molars in 100 subjects aged 14 years at baseline. sites were monitored at 6-monthly intervals for clinical loss of attachment. six subjects showed greater than 1 mm loss of attachment at one site 18-24 months later. further samples were obtained from these sites together with a comparison sample from the contralateral site and equivalent sites in a matched control subject. actinobacillus actinomycetemcomitans was more fre ...19892640319
clinical, microbiological and immunological studies of post-juvenile periodontitis.the present study includes the clinical, microbiological and immunological examinations of 2 patients with post-juvenile periodontitis. bacteroides intermedius was the predominant isolate from periodontal pockets with post-localized juvenile periodontitis. bacteroides gingivalis, bacteroides forsythus and actinobacillus actinomycetemcomitans were detected in samples from periodontal pockets with post-generalized juvenile periodontitis. igg antibody levels to b. gingivalis were significantly high ...19892640920
hormonal influences on gingival tissue: relationship to periodontal disease.it is the purpose of this review to survey the influence of corticosteroids, androgens, oestrogens and progesterone on gingival tissues and to show the relationship of such influences to periodontal disease. the clinical changes seen in plaque-induced gingivitis are accentuated by circulating levels of the above hormones via mechanisms such as partial immune suppression, increased fluid exudation, stimulation of bone resorption and stimulation of fibroblast synthetic activity. high counts of bac ...19892654195
a comparison of fibronectinolytic activities from several oral bacteria.fibronectinolytic activity from two gram-positive microorganisms (streptococcus mutans and bacterionema matruchotii), and from three gram-negative oral bacteria (bacteroides intermedius, bacteroides gingivalis and haemophilus actinomycetemcomitans) were compared. 125i-labelled human plasma fibronectin (fn) was incubated either either with bacterial extracts or with concentrated culture medium samples and the patterns of fn-degradation products were determined by sds-page. results to date have sh ...19892693452
[microbiological and serological investigation of periodontal disease activity].if disease activity in periodontal disease can be determined, the therapeutic measures of periodontitis may become diverse and different from patient to patient. the purpose of the present study was to evaluate the usefulness of microbiological and serological examination of periodontopathic bacteria in detecting destructive periodontal disease activity. one hundred and forty-eight sites in 52 subjects with moderate to severe periodontitis were studied clinically. clinical parameters included pl ...19892700196
[microbiological evaluation of initial preparation for adult periodontitis patients].the microbial flora from 46 adult periodontitis lesions of 23 patients and 18 sites in 9 healthy persons were examined and levels of serum igg antibody to gram negative periodontal disease-associated bacteria were measured by enzyme-linked immunosorbent assay. plaque samples and serum samples were taken 40-50 days after initial preparation consisting of scaling and root planing. to evaluate the effects of the therapy on 10 patients with adult periodontitis, changes in clinical parameters were co ...19892700354
[microbial flora of periodontitis and monitoring of the effect of initial preparation by immunofluorescence microscopy].the effect of initial preparation in adult periodontitis was evaluated by changes in clinical parameters and immunofluorescence microscopic counts of periodontal disease associated bacteria. subgingival plaque samples were taken with sterilized paper points from 10 sites of 5 periodontally healthy persons and 44 sites of 23 adult periodontitis patients. twenty-one diseased sites were periodically examined after plaque control and scaling, root planing. the direct immunofluorescence technique was ...19892700361
neuraminidase-enhanced attachment of bacteroides intermedius to human erythrocytes and buccal epithelial cells.bacteroides intermedius strains strongly agglutinated only neuraminidase-treated erythrocytes. the neuraminidase-dependent hemagglutinating activity of b. intermedius was abolished by heating or treating with protease. the adherence of these microorganisms to human buccal epithelial cells was enhanced by neuraminidase pretreatment of the cells (p less than 0.01).19892707865
periodontal condition and microbiology of healthy and diseased periodontal pockets in type 1 diabetes mellitus patients.on the basis of glycosylated hemoglobin (hba1c) values, 22 type 1 (insulin-dependent) diabetic adults were grouped into patients with near normal (hba1c less than or equal to 7.7%) and poor (hba1c greater than or equal to 9.9%) metabolic control. a total of 44 subgingival sites were examined for actinobacillus actinomycetemcomitans, black-pigmented bacteroides species and capnocytophaga species. no significant difference could be demonstrated between patients in the 2 test groups with regard to ...19892723104
taxonomy, virulence and epidemiology of black-pigmented bacteroides species in relation to oral infections.black-pigmented bacteroides species are recognized as suspected pathogens of oral infections. developments in the taxonomy of this group include description of a new asaccharolytic species, bacteroides salivosus, and proposal for the reclassification of the asaccharolytic species into a separate genus, porphyromonas. studies on the pathogenicity and virulence of black-pigmented bacteroides species have identified bacteroides gingivalis as the most virulent species. b. gingivalis and bacteroides ...19892737764
activity of amine-stannous fluoride combination and chlorhexidine against some aerobic and anaerobic oral bacteria.the in vitro susceptibility of 128 bacterial strains was tested to amine fluoride-stannous fluoride (amf + snf) and chlorhexidine (chx) solutions. actinobacillus actinomycetemcomitans, streptococcus mutans, and bacteroides intermedius were among the species investigated. the 50% and 90% minimal inhibitory concentrations (mics) were assessed by an agar dilution method. the mic ranges for a. actinomycetemcomitans were 2-32 micrograms/ml for chx and 0.25-64 micrograms/ml for amf + snf. the respecti ...19892762015
growth and metabolic properties of bacteroides intermedius in anaerobic continuous culture.two strains of bacteroides intermedius bh20/30 and bh18/23, have been grown in anaerobic continuous culture under various conditions for periods up to 54 days. strain bh20/30 grew over a relatively wide ph range from 5-8 with a maximum at ph 7.0 at a dilution rate (d) of 0.1 h-1 with a glucose limitation, while strain bh18/23 had an optimum between 5.8 and 7.3 and would not grow above and below this range. the maximum growth rate (mu max) for the latter strain was shown to be 0.23 h-1, or a doub ...19892762020
experimentally induced subcutaneous infection by black pigmented bacteroides species in rats.in a rat model of anaerobic subcutaneous abscess developed to study the pathogenicity of pure cultures of black pigmented bacteroides sp. (bacteroides melaninogenicus and bacteroides intermedius) maximum lesions were seen between 7 and 15 days after inoculation. abscesses were produced by one type strain each of b. melaninogenicus and b. intermedius and by 20 of 25 clinical isolates of black pigmented bacteroides species.19852865315
use of the rapid-ana system to screen for enzyme activities that differ among species of bile-inhibited bacteroides.the rapid-ana system (innovative diagnostics systems, inc., atlanta, ga.) was used to test 102 strains of 14 species of phenotypically similar bile-inhibited bacteroides from humans. bacteroides oris, bacteroides veroralis, bacteroides buccalis, bacteroides melaninogenicus, bacteroides loescheii, and bacteroides denticola had very similar enzyme activity profiles. clear differentiation of these six species by the rapid-ana system was not possible, but tests for arginine aminopeptidase and beta-g ...19862871042
an electron microscope survey of the surface structures and hydrophobicity of oral and non-oral species of the bacterial genus bacteroides.seventeen strains of bacteroides representing 10 species were examined by negative staining; the majority were from the mouth but a few non-oral strains were included. seven species had peritrichously-arranged, non-flagellar appendages which could be divided by morphology and ultrastructure into two subgroups, fibrils and fimbriae. bacteroides asaccharolyticus strains b536 and b537 and bacteroides gingivalis strains w50, w83, wph15 and wph35 had fimbriae with mean width of 4.4 nm and 0.5 to 6.0 ...19862875705
the use of whole-cell dna probes for the identification of bacteroides intermedius isolates in a dot blot assay.bacteroides intermedius includes two distinct groups of organisms that are phenotypically indistinguishable by conventional methods. these two groups are represented by the type strain of the species atcc 25611t (b. intermedius type i) and by atcc 33563 (b. intermedius type ii). members of each group can be distinguished from each other by analysis of the cellular protein composition by sds-polyacrylamide gel electrophoresis (sds-page) and by dna-dna homology studies, because they share less tha ...19882902111
effects of endotoxins from bacteroides intermedius and escherichia coli on human monocytes in vitro.endotoxins (lipopolysaccharides, lpss) from the strictly anaerobic bacterium bacteroides intermedius and from escherichia coli were compared with respect to effects on human monocytes. exposure of monocyte monolayer cultures to the structurally different endotoxins caused morphological changes, demonstrated by large rounded cells containing cytoplasmic granules with the b. intermedius lps, and smaller cells with several cytoplasmic protrusions with the e. coli lps after 10 days of culture. the b ...19872964769
bacteroides intermedius binds fibrinogen.the binding of bacteroides intermedius vpi 8944 to human fibrinogen has been characterized. the binding is time dependent, at least partially reversible, saturable, and specific. on an average, a maximum of 3,500 fibrinogen molecules bind per bacterial cell, with a dissociation constant of 1.7 x 10(-11) m. these bacteria also exhibit a fibrinogenolytic activity which can be partially inhibited by protease inhibitors. bacteria release fibrinogenolytic activity into the surrounding medium without ...19852991200
correlations between gingival crevicular fluid enzymes and the subgingival microflora.bacteroides gingivalis is a gram-negative micro-organism implicated in the pathogenesis of adult periodontitis and producing relatively large amounts of specific enzymes. in the present study, subgingival samples taken from adults with moderate periodontitis were examined for the presence and relative amounts of enzymatic activity toward certain substrates. enzyme levels were then correlated with clinical periodontal indices and microbiological analysis of subgingival plaque, including darkfield ...19883042823
antimicrobial agents in the treatment of periodontal diseases: special aspects on tetracycline and doxycycline.increasing evidence for the involvement of specific microorganisms in the etiology of human periodontal diseases has appeared during the last few years. the microorganisms most likely to cause periodontal disease are actinobacillus actinomycetemcomitans, bacteroides gingivalis, bacteroides intermedius and capnocytophaga species. many of the strains are resistant to tetracycline and doxycycline. recently penicillin-resistant b. gingivalis and b. intermedius have also been described. a. actinomyce ...19883047856
[prevalence and identification of serogroups of bacteroides intermedius isolated from human gingival crevice]. 19883075720
the effect of lowering the ph on the composition and metabolism of a community of nine oral bacteria grown in a chemostat.nine oral bacteria, associated with both healthy and diseased sites in the mouth, were grown at d = 0.05 h-1 (mean generation time 13.9 h) in a glucose-limited chemostat. after an initial period of steady-state growth at ph 7.0, ph control was discontinued. the ph then decreased until it stabilized at ph 4.1 after 9 d (16 generations), while the eh rose from -165 mv to +160 mv. the lowering in ph resulted in the composition and metabolism of the flora being altered and in increased bacterial agg ...19863095488
interactions of bacteroides gingivalis with fibrinogen.results of previous studies from our laboratory have shown that a strain of bacteroides intermedius isolated originally from a patient with acute necrotizing ulcerative gingivitis binds and degrades human fibrinogen (m.s. lantz, l.m. switalski, k.s. kornman, and m. hook, j. bacteriol. 163:623-628, 1985). we report that strains of bacteroides gingivalis, an organism implicated in the etiology of several forms of periodontitis, also bind and degrade fibrinogen. the binding is rapid, reversible, sa ...19863096886
serological studies of oral bacteroides intermedius.bacteroides intermedius is a gram negative, anaerobic microorganism associated with certain forms of human periodontal disease, including adult periodontitis and acute necrotizing ulcerative gingivitis. previous studies have indicated the presence of two dna homology groups which could be distinguished by analysis of protein patterns on polyacrylamide gel electrophoresis, as well as at least two serogroups within b. intermedius. the present study examined the serology of b. intermedius and deter ...19883131249
gamma interferon modulation of prostaglandin e2 release from monocytes stimulated with lipopolysaccharides from bacteroides intermedius, bacteroides gingivalis, and salmonella typhimurium. 19883152029
comparison of cultural methods and dna probe analyses for the detection of actinobacillus actinomycetemcomitans, bacteroides gingivalis, and bacteroides intermedius in subgingival plaque samples.the purpose of this study was to compare dna probe analyses to cultural methods for detecting three periodontal pathogens, actinobacillus actinomycetemcomitans, bacteroides gingivalis, and bacteroides intermedius, in human subgingival plaque. subgingival sites from patients diagnosed as either healthy or showing evidence of gingivitis or juvenile or adult periodontitis were sampled using two paper points. the number of these pathogens from one paper point was determined using microbiologic media ...19883166055
the comparative cytotoxicity of periodontal bacteria.the direct cytotoxicity of sonic extracts (se) from nine periodontal bacteria for human gingival fibroblasts (hgf) was compared. equivalent dosages (in terms of protein concentration) of se were used to challenge hgf cultures. the cytotoxic potential of each se was assessed by its ability to (1) inhibit hgf proliferation, as measured by direct cell counts; (2) inhibit 3h-thymidine incorporation in hgf cultures; or (3) cause morphological alterations of the cells in challenged cultures. the highe ...19883204481
relationships between periodontal health, salivary steroids, and bacteroides intermedius in males, pregnant and non-pregnant women.relationships between four steroids, determined by radio-immunoassay of whole saliva, and clinical and bacteriological parameters were studied in 90 subjects: males, menstruating females, and pregnant females. pocket depths and both plaque and gingival bleeding scores were recorded. total counts and percentages of gram-negative organisms bacteroides and b. intermedius were determined from anaerobic cultures of subgingival plaque from 9-14 subjects in each group. none of the clinical parameters f ...19883261302
fatty acids and sugars in lipoplysaccharides from bacteroides intermedius. bacteroides gingivalis and bacteroides loescheii. 19883268745
the occurrence of actinobacillus actinomycetemcomitans, bacteroides gingivalis, bacteroides intermedius and spirochaetes in the subgingival microflora of adolescents and their relationship with the amount of supragingival plaque and gingivitis. 19883268755
[colonization mechanism of bacteroides intermedius]. 19883269631
microbiological and immunological studies of adult periodontitis in patients with noninsulin-dependent diabetes mellitus.the subgingival microflora and serum antibody response was examined in periodontitis patients with noninsulin-dependent diabetes mellitus (niddm), impaired glucose tolerance (igt), and normal glucose tolerance (ngt). the predominant cultivable microflora was determined for subgingival plaque sampled from two deep periodontal pockets in each of eight adult periodontitis patients with niddm. indirect immunofluorescence for bacteroides intermedius, bacteroides gingivalis, and haemophilus actinomyce ...19883276868
bacteroides gingivalis, bacteroides intermedius and actinobacillus actinomycetemcomitans in human periodontal diseases.bacteroides gingivalis, bacteroides intermedius and actinobacillus actinomycetemcomitans seem to be major pathogens in advancing periodontitis in man. first, these organisms are recovered in higher prevalence and proportions from progressive periodontitis lesions than from quiescent periodontal sites. second, antibody levels against b. gingivalis and a. actinomycetemcomitans are markedly elevated in serum and gingival crevice fluid of periodontitis patients compared to normal controls. third, b. ...19883279073
the role of black-pigmented bacteroides in human oral infections.today, 10 black-pigmented bacteroides (bpb) species are recognized. the majority of these species can be isolated from the oral cavity. bpb species are involved in anaerobic infections of oral and non-oral sites. in the oral cavity, bpb species are associated with gingivitis, periodontitis, endodontal infections and odontogenic abscesses. cultural studies suggest a specific role of the various bpb species in the different types of infection. bacteroides gingivalis is closely correlated with dest ...19883280611
toxic effects of lipopolysaccharide from bacteroides intermedius and escherichia coli assessed in the pre-implantation mouse embryo culture system.a comparative study on the toxic effects of endotoxin (lipopolysaccharide, lps) from the strictly anaerobic bacteroides intermedius bm1 and from escherichia coli 055 was performed. pre-implantation mouse embryos at the 2-cell stage from lps responder (c57bl/6j) and low responder (c3h/hej) mouse strains were exposed to the endotoxins, and development was observed during 72 h in vitro. toxic effects of both endotoxins on embryos from both mouse strains were demonstrated. b. intermedius lps was les ...19873296652
effects of endotoxins from bacteroides intermedius and escherichia coli on cytotoxic and lysosomal activity in peritoneal macrophages from endotoxin responder and low responder mouse strains.peritoneal macrophages from normal mice strains (c3h/tif and c57bl/6j) and from the endotoxin (lipopolysaccharide, lps) low responder strain (c3h/hej were exposed to two structurally different endotoxins from bacteroides intermedius and escherichia coli in vitro. intracellular activity of a lysosomal enzyme (acid phosphatase) and macrophage mediated cytotoxic activity against a tumor cell line (l929) were tested. both endotoxins caused increased levels of acid phosphatase activity in normal mice ...19873307300
natural occurrence of black-pigmented bacteroides species in the gingival crevice of the squirrel monkey.the objective of this study was to determine whether the squirrel monkey (saimiri scuireus) is indigenously colonized with black-pigmented bacteroides (bpb) resembling human bacteroides gingivalis and bacteroides intermedius (suspected periodontal pathogens) and to determine the usefulness of the squirrel monkey as an in vivo model for studying colonization by putative pathogens. we assayed the subgingival plaques of 138 monkeys of various ages and in four different colonies for the presence of ...19883410543
isolation of intact high-molecular-weight dna by using guanidine isothiocyanate.a method for obtaining high-molecular-weight chromosomal dna from bacteroides intermedius and bacteroides gingivalis is described. this technique is a modification of the guanidine isothiocyanate isolation procedure for rna and should be useful for isolating intact dna from organisms with high endogenous nuclease activity.19873426222
subinhibitory concentrations of tetracycline alter fibrinogen binding by bacteroides intermedius.previous studies from our laboratory have shown that a strain of bacteroides intermedius, vpi 8944, an organism isolated originally from a patient with acute necrotizing ulcerative gingivitis, binds human fibrinogen rapidly, reversibly, specifically, saturably, and with high affinity (m.s. lantz, l.m. switalski, k.s. kornman, and m. höök, j. bacteriol. 163:623-628, 1985). we examined the effect of growth in subinhibitory levels (sub-mics) of tetracycline on fibrinogen binding by these bacteria a ...19873439801
studies on bacterial synergism in mice infected with bacteroides intermedius and fusobacterium necrophorum.this study was undertaken to characterize the kinetics of possible bacterial synergy using a mouse model of mixed intraabdominal infection with bacteroides intermedius and fusobacterium necrophorum. female cd-1 mice were injected intraperitoneally with b. intermedius, f. necrophorum or mixtures of both organisms. generalized septic peritonitis developed within 24 hr, with abscess formation occurring after one to two wk in survivors with the mixed infection. involvement of the reticuloendothelial ...19873449614
bacterial adherence to gingival epithelial cells of rats with naturally occurring gingivitis.bacterial adherence to gingival cells was compared in rats with spontaneous gingivitis (sus rat) and in control rats (res rat). in the sus rat, the number of adherent bacteria in gingival cells changed according to the progression of gingival inflammation. there was a correlation between the change of the number of adherent bacteria, progress of inflammation and ph in the pocket. in the res rat, the number of adherent bacteria was constant and very small. in the adherence assay, adherence of bac ...19863457144
the occurrence of actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius in destructive periodontal disease in adults.a total of 235 subgingival sites, including 104 progressive deep lesions from 61 untreated patients, 26 progressive deep lesions from 10 treated patients, 33 nonprogressive deep sites from 20 untreated patients, and 72 nonprogressive sites from 55 treated patients were examined for actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. the periodontal disease progression was mainly determined on the basis of radiographic changes in the crestal alveolar bone lev ...19863462204
bacterial specificity in adult periodontitis. a summary of recent work.3 studies investigated the importance of actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius in progressive adult periodontitis. a total of 196 adults with advanced periodontitis and 475 periodontal sites were examined. the periodontal disease activity was assessed by changes in probing periodontal attachment level and crestal alveolar bone level. the composition of the subgingival microflora was determined by nonselective and selective culturing and indirect ...19863466908
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