Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
bacterial flora of hirudo medicinalis and their antibiotic sensitivities in the middle black sea region, turkey.the rate of infectious complications of leech therapy is almost 20% because hirudo medicinalis has endosymbiotic bacteria. the aim of this study was to determine the bacterial flora of h. medicinalis and their antibiotic sensitivities in a region in turkey. sixteen adult leeches were collected in middle black sea region, turkey. they were rubbed onto blood agar plates directly under ether anesthesia to obtain surface cultures. they were then killed to obtain mouth and gut cultures. culture swabs ...200111756807
particulate malate oxidation in strictly aerobic bacteria: the respiratory chain of moraxella lwoffi. 197211946616
gastritis and hypergastrinemia due to acinetobacter lwoffii in mice.in mouse models and humans, helicobacter pylori is associated with an increase in serum gastrin and gastrin-expressing (g) cells with a concomitant decrease in somatostatin-expressing d cells. inflammation of the gastric mucosa can progress to metaplastic changes in the stomach and to decreased colonization by h. pylori and increased colonization by non-h. pylori organisms. in addition, about 20% of individuals with chronic gastritis are h. pylori negative, suggesting that other organisms may in ...200211953405
[post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria].as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, monobactams, and carbapenems. changes in czop susceptibility for the bacteria were also evaluated with the bacterial resistance ratio calculated with the breakpoint mic. twenty-five species (3,362 strains) of gram-negative bacteria were isolated from the clinica ...200211977920
purification and characterization of an esterase from acinetobacter lwoffii i6c-1.esta was purified from the supernatant by a. lwoffii 16c-1. its molecular mass was determined to be 45 kda, and the optimal activity occurred when the ph level was 8.0 at a temperature of 37 degrees c. the activation energies for the hydrolysis of p-nitrophenyl butyrate was determined to be 11.25 kcal/mol in the temperature range of 10-37 degrees c. the enzyme was unstable at temperatures higher than 50 degrees c. the michaelis constant ( k(m)) and v(max) for p-nitrophenyl butyrate were 11 mocro ...200212000989
antimicrobial multiresistance in bacteria isolated from freshwater chilean salmon farms.the intensive use of antimicrobial agents, mainly oxytetracycline, to prevent and control bacterial pathologies in chilean salmon culture is a frequent practice. a total of 103 gram-negative oxytetracycline-resistant bacteria recovered from various sources of 4 chilean freshwater salmon farms were identified and investigated for their susceptibility patterns to various antibacterial agents, by using an agar disk diffusion method. antibacterial resistance patterns of isolates were not correlated ...200212109474
enhanced detection and characterization of protocatechuate 3,4-dioxygenase in acinetobacter lwoffii k24 by proteomics using a column separation.acinetobacter lwoffii k24 known as an aniline degrading bacterium has also been found to utilize p-hydroxybenzoate as a sole carbon source. in this study, 2-de using q-sepharose column separation was attempted for fast screening of protocatechuate 3,4-dioxygenase for catabolism of p-hydroxybenzoate in a. lwoffii k24. two protocatechuate 3,4-dioxygenase subunits, pcag and pcah were detected and identified with n-terminal and internal sequencing, suggesting proteomics using a column separation may ...200212127980
comparative in vitro activity of faropenem and 11 other antimicrobial agents against 405 aerobic and anaerobic pathogens isolated from skin and soft tissue infections from animal and human bites.faropenem, a new oral beta-lactam agent with a penem structure, was very active against 405 aerobic and anaerobic bite isolates. it inhibited 232 of 236 (98%) aerobic isolates, including all pasteurella spp. and eikenella corrodens at < or = 0.25 mg/l, and 164/169 (97%) anaerobic isolates, at < or = 1 mg/l. the 10 isolates that required > or = 2 mg/l for inhibition were one strain each of acinetobacter lwoffi, corynebacterium minutissimum, bacteroides ovatus, lactobacillus delbrueckii and peptos ...200212205068
compositions and the in vitro antimicrobial activities of the essential oils of achillea setacea and achillea teretifolia (compositae).gc-ms analysis of the isolated essential oils from air-dried aerial parts of achillea setacea and achillea teretifolia, an endemic taxon, resulted in the identification of 51 constituents (79.8% of the total oil) and 42 constituents (87.1% of the total oil), respectively. eucalyptol (1,8-cineole) was the major constituent of both oils studied (18.5 and 19.9%, respectively). the antimicrobial activities of the essential oils were individually evaluated against 14 microorganisms. both oils exhibit ...200212413716
involvement of a protein tyrosine kinase in production of the polymeric bioemulsifier emulsan from the oil-degrading strain acinetobacter lwoffii rag-1.the genes associated with the biosynthesis of the polymeric bioemulsifier emulsan, produced by the oil-degrading acinetobacter lwoffii rag-1 are clustered within a 27-kbp region termed the wee cluster. this report demonstrates the involvement of two genes of the wee cluster of rag-1, wzb and wzc, in emulsan biosynthesis. the two gene products, wzc and wzb were overexpressed and purified. wzc exhibited atp-dependent autophosphorylating protein tyrosine kinase activity. wzb was found to be a prote ...200312533476
microbial contamination of non-disposable instruments in otolaryngology out-patients.nosocomial infections are an important cause of morbidity and contaminated equipment may contribute to this. there has been little work concerning contamination of non-disposable equipment in otolaryngology. this study investigates the efficacy of the cleaning regimen for non-disposable instruments in an otolaryngology out-patients clinic. instruments were swabbed before each clinic, when they had been autoclaved, then after use on patients, when they had been washed with detergent and chlorhexi ...200312625885
gene cloning, sequencing, and expression of an esterase from acinetobacter lwoffii i6c-1.the esterase-encoding gene, esta, was cloned from acinetobacter lwoffii i6c-1 genomic dna into escherichia coli bl21(de3) with plasmid vector pet-22b (pem1). pem1 has a 4.4-kb ecori insert that contained the complete esta gene. a 2.4-kb avai- sphi dna fragment was subcloned (pem3) and sequenced. esta gene encodes a protein of 366 amino acids (40,687 da) with a pi of 9.17. the esta signal peptide was 31 amino acids long, and the mature esterase sequence is 335 amino acids long (37.5 kda). the con ...200312732980
acinetobacter lwoffii infection and gastritis.about 25% of humans with chronic gastritis are negative for helicobacter pylori, suggesting that other bacteria are capable of causing inflammation. bacterial overgrowth may occur in the stomach under conditions of reduced acid secretion. in this review, we will explore what is generally known about non-h. pylori organisms and their ability to induce gastritis, with particular focus on acinetobacter lwoffi.200312787741
[characteristics of strains of acinetobacter spp. after culture in various media].growth, surface properties, production of lipase, histamine and some signal molecules (n-acyl-homoserine lactones, ahls) in four acinetobacter baumannii strains, in one acinetobacter lwoffii and in one acinetobacter haemolyticus strain cultivated in three complex media were studied. more intensive growth of bacterial suspensions was observed in mueller-hinton broth and in brain heart infusion in comparison with growth in proteose peptone. greater growth of bacterial strains (with the exception o ...200312822537
antioxidant and antimicrobial activity of the essential oil and methanol extracts of achillea millefolium subsp. millefolium afan. (asteraceae).the in vitro antimicrobial and antioxidant activities of the essential oil and methanol extracts of achillea millefolium subsp. millefolium afan. (asteraceae) were investigated. gc-ms analysis of the essential oil resulted in the identification of 36 compounds constituting 90.8% of the total oil. eucalyptol, camphor, alpha-terpineol, beta-pinene, and borneol were the principal components comprising 60.7% of the oil. the oil strongly reduced the diphenylpicrylhydrazyl radical (ic(50)=1.56 micro g ...200312860311
a comparison of the bacteria found on the hands of 'homemakers' and neonatal intensive care unit nurses.this prevalence study was conducted to compare the counts, types and antimicrobial resistance profiles of bacterial flora on the hands of individuals in the community to that of nurses at a nearby university teaching hospital, with an intense hand hygiene regimen. hand cultures were obtained from 204 individuals during a home visit and 119 nurses in two neonatal intensive care units (nicus). the mean total log counts of organisms were 5.73 and 5.24 for the homemakers [defined as the person (usua ...200312919763
[biochemical identity between certain strains of b. anitratum and moraxella lwoffi]. 195212976774
[pathogenic or saprophytic bacteria transforming glucose to gluconic acid, bacterium anitratum, b5w, moraxella lwoffi var. glucidolytica, neisseria winogradskyi]. 195413171653
[case of cerebrospinal meningitis from moraxella lwoffi in viet nam]. 195413182498
[proteolytic types of moraxella lwoffi and moraxella glucidolytica (bacterium anitratum)]. 195613327511
[septicemic state due to moraxella lwoffi]. 195613335885
[purulent pleurisy from moraxella lwoffi; presence of the bacterium in the blood]. 195613380077
[meningitis caused by moraxella lwoffi]. 195713454228
[pathogenic properties of moraxella lwoffi]. 195713471085
[intestinal infections by alcaligenes faecalis & their analogy with intestinal syndromes due to moraxella lwoffi]. 195813534033
[two cases of meningitis caused by acinetobacter lwoffi (ex moraxella)]. 195913671347
[bacteriological characteristics of 214 strains of moraxella lwoffi and m. glucidolytica (acinetobacter)]. 196113695993
[septicemic state caused by acinetobacter lwoffi]. 196013767606
[on the possible development of so-called terminal endocarditis in bacterial endocarditis. apropos of a case of endocarditis with non-glucidolytic moraxella lwoffi]. 196113871599
[multiple myocardial abscess caused by acinetobacter lwoffi]. 196213933173
gliding motility in the organisms bacterium anitratum (b5w), moraxella lwoffi and alkaligenes haemolysans, as compared to moraxella nonliquefaciens. 196314083764
[mima polymorpha (moraxella lwoffi) as the etiological agent of urethritis]. 196414134299
serology of the mima-herellea group and the genus moraxella.mitchell, paul d. (west virginia university medical center, morgantown), and robert g. burrell. serology of the mima-herellea group and the genus moraxella. j. bacteriol. 87:900-909. 1964.-the identity of organisms which have been assigned to the mima-herellea group of the tribe mimeae and their taxonomic position are uncertain. in considering the possible relationship of the mima-herellea group to the genus moraxella, the approach used was the isolation and identification of representative anti ...196414137629
[the link of bacterium anitratum, moraxella lwoffi, bacillus mallei and haemophilus parapertussis to the acinetobacter brisou and pr'evot genus]. 196414141566
[acute purulent meningitis due to moraxella lwoffi]. 195514395157
[on the systematology of bacterium anitratum, diplococcus mucosus and moraxella lwoffi. (genus acinetobacter according to brisou and prevot)]. 195914409896
[incidence of diseases caused by moraxella lwoffi and moraxella glucidolytica. study of the sensitivity to antibiotics of 28 strains isolated in new caledonia]. 195914431511
[study of 5 strains of moraxella lwoffi (acinetobacter lwoffi) isolated from pathological material]. 196114448658
[potassium ion and growth of the bacteria moraxella lwoffi]. 195014771602
[acute purulent meningitis from moraxella lwoffi; behavior of this bacteria towards antibiotics]. 195014801706
non-fermenters in human infections.one thousand and one hundred thirty non-fermenting gram negative bacteria were isolated from various samples. of these, pseudomonas aeruginosa was the commonest isolate (72.83%) followed by acinetobacter anitratus (8.4%), alcaligenes faecalis (7.6%), acinetobacter lwoffi (4.4%), pseudomonas flourescens (2.4%), schwanella putrefaciens (1.6%), stenotrophomonas maltophilia (1.6%), pseudomonas putida (0.4%), bravundimonas vesicularis (0.4%) and flavobacterium meningosepticum (0.4%). antibiotic sensi ...200315022936
bacteremia in cirrhotic patients submitted to endoscopic band ligation of esophageal varices.endoscopic procedures can develop bacteremia. patients with chronic liver disease are more predisposed to undergo bacteremia and infections because they are immunocompromised.200415029392
proteomic analysis of acinetobacter lwoffii k24 by 2-d gel electrophoresis and electrospray ionization quadrupole-time of flight mass spectrometry.the ms/ms analysis by electrospray ionization quadrupole-time of flight mass spectrometry (esi-q-tof ms) was applied to identify proteins in proteome analysis of bacteria whose genomes are not known. the protein identification by esi-q-tof ms was performed sequentially by database search and then de novo sequencing using ms/ms spectra. soil bacteria having unanalyzed genome, acinetobacter lwoffii k24 is an aniline degrading bacterium. in this report, we present the results of a comparison betwee ...200415134882
in vitro antimicrobial activity of gatifloxacin compared with other quinolones against clinical isolates from cancer patients.owing to the predominance of gram-positive pathogens in neutropenic cancer patients, newer generation quinolones with an expanded gram-positive spectrum and enhanced potency, may have a role to play for prophylaxis and/or empiric therapy in such patients. the in vitro activity of gatifloxacin was compared with that of ciprofloxacin, levofloxacin and trovafloxacin against 848 recent clinical isolates from cancer patients. against gram-positive organisms, gatifloxacin was the most active agent tes ...200415523180
inactivation of gram-negative and gram-positive bacteria in red cell concentrates using inactine pen110 chemistry.the risk of transfusion-transmitted bacterial infections as a result of the presence of bacteria in blood is one of the major concerns in transfusion medicine. the purpose of this study was to investigate whether bacteria inoculated into red blood cell concentrates can be inactivated by the inactine pen110 pathogen-reduction process. four bacterial species were chosen for the study: anaerobic gram-positive clostridium perfringens and propionibacterium acnes, known to be transfusion-transmitted; ...200415569065
bacteriospermia in extended porcine semen.bacteriospermia is a frequent finding in freshly extended porcine semen and can result in detrimental effects on semen quality and longevity if left uncontrolled. the primary source of bacterial contamination is the boar. other sources that have been identified include environment, personnel, and the water used for extender preparation. a 1-year retrospective study was performed on submissions of extended porcine semen for routine quality control bacteriological screening at the university of pe ...200515626417
design and application of an oligonucleotide microarray for the investigation of compost microbial communities.a microarray consisting of oligonucleotide probes targeting variable regions of the 16s rrna gene was designed and tested for the investigation of microbial communities in compost. probes were designed for microorganisms that have been previously reported in the composting process and for plant, animal and human pathogens. the oligonucleotide probes were between 17 and 25 bp in length and included mostly species-specific sequences. validation of probe specificity and optimization of hybridizatio ...200515823393
primary cutaneous cryptococcosis and secondary antigenemia in a patient with long-term corticosteroid therapy.we report a case of a 71-year-old male who developed severe cellulitis of his right forearm and hand after he had an accidental injury from the sharp edge of a metal plate of a birdhouse. the patient suffers from chronic asthma and has been treated with systemic corticosteroids for years. culture of aspirates from two sites of the wound area revealed growth of cryptococcus neoformans in one and acinetobacter lwoffii in the other. after combined treatment including antibiotics, antifungal therapy ...200515827877
gram-negative bacilli associated with catheter-associated and non-catheter-associated bloodstream infections and hand carriage by healthcare workers in neonatal intensive care units.bloodstream infections caused by gram-negative bacilli are a substantial cause of morbidity and mortality in infants in neonatal intensive care units. this study describes the species of gram-negative bacilli causing bloodstream infections in two neonatal intensive care units, compares characteristics of catheter-related and non-catheter-related bloodstream infections, and compares species and antibiotic resistance patterns of these organisms with those isolated from the hands of nurses working ...200515982435
isolation of acinetobacter lwoffii from a lovebird (agapornis roseicollis) with severe respiratory symptoms.although acinetobacter lwoffii is generally considered an ubiquitous and opportunistic bacterium, this germ has been isolated from the pulmonary and abdominal air sac swabs obtained from a lovebird (agapornis roseicollis), which died of a severe respiratory disease. bacteriological tests (phenotypic and genotypic) led to the identification of a. lwoffii in pure culture. all the other parrots in the breeding centre were treated orally with oxytetracycline for 14 days and 3 months later no bird sh ...200515999637
preseptal cellulitis caused by acinetobacter lwoffi. 200516137978
differential organization and transcription of the cat2 gene cluster in aniline-assimilating acinetobacter lwoffii k24.catabc genes encode proteins that are responsible for the first three steps of one branch of the beta-ketoadipate pathway involved in the degradation of various aromatic compound by bacteria. aniline-assimilating acinetobacter lwoffii k24 is known to have the two-catabc gene clusters (cat1 and cat2) on the chromosome (kim et al., j. bacteriol., 179: 5226-5231, 1997). the order of the cat2 gene cluster is catb2a2c2, which has not been found in other bacteria. in this report, we analyzed the trans ...199916232607
prosthetic valve endocarditis caused by acinetobacter lwoffi: a case report and review.we are reporting a case of recurrent prosthetic-valve endocarditis (pve) caused by an unusual pathogen. the patient suffered 2 consecutive relapses of acinetobacter lwoffi bacteremia, although he had completed a full course of treatment with antibiotics to which the microorganism was susceptible. he was finally successfully operated with replacement of the infected aortic valve. acinetobacter spp are relatively low-grade but potentially virulent pathogens, and endocarditis caused by these specie ...200616371766
helicobacter pylori-secreted factors inhibit dendritic cell il-12 secretion: a mechanism of ineffective host defense.helicobacter pylori evades host immune defenses and causes chronic gastritis. immunity against intestinal pathogens is largely mediated by dendritic cells, yet the role of dendritic cells in acute h. pylori infection is largely unknown. we observed the recruitment of dendritic cells to the gastric mucosa of h. pylori-infected mice. bone marrow-derived dendritic cells from mice responded to live h. pylori by upregulating the expression of proinflammatory cytokine mrna (i.e., il-1alpha, il-1beta, ...200616469828
biodegradation of phthalate isomers by pseudomonas aeruginosa pp4, pseudomonas sp. ppd and acinetobacter lwoffii isp4.pseudomonas aeruginosa pp4, pseudomonas sp. ppd and acinetobacter lwoffii isp4 capable of utilizing phthalate isomers were isolated from the soil using enrichment culture technique. the strain isp4 metabolizes isophthalate, while ppd and pp4 utilizes all three phthalate isomers (ortho-, iso- and tere-) as the sole carbon source. isp4 utilizes isophthalate (0.1%) more rapidly (doubling time, 0.9 h) compared to ppd (4.64 h), pp4 (7.91 h) and other reported strains so far. the metabolic pathways in ...200616607524
characterization of acinetobacter from clinical isolates at gandhi memorial and associated hospitals, lucknow.the study was conducted in 4140 clinical samples sent to microbiology department from different department of g.m. and associated hospitals. the samples included 2270 urine, 960 pus, 300 blood, 210 sputum, 180 csf, 20 intercostal drainage tubes and 150 other swabs like vaginal and urethral, conjunctival smear 30, 10 ascitic fluids and 10 gastric aspirates. apart from this, 30 specimens were collected from hospitals environment, like linen and trolley. from clinical samples, 43 acinetobacter stra ...200516637397
characterization and changing minimum inhibitory concentration (mic) of acinetobacter species from a tertiary care setup.128 isolates of acinetobacter species from admitted and outdoor patients were subjected to biotyping and resistotyping. resistance phenotype analysis included nine antibiotics and two betalactam inhibitor combination drugs. in 100 strains of acinetobacter spp. ciprofloxacin, amikacin, cefotaxime and cefepime minimum inhibitory concentration (mic) was done by agar dilution using nccls 2002 criteria. in forty-nine isolates mic level was determined by e-strip also. extended spectrum beta lactamase ...200516749272
enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms.most biofilms in their natural environments are likely to consist of consortia of species that influence each other in synergistic and antagonistic manners. however, few reports specifically address interactions within multispecies biofilms. in this study, 17 epiphytic bacterial strains, isolated from the surface of the marine alga ulva australis, were screened for synergistic interactions within biofilms when present together in different combinations. four isolates, microbacterium phyllosphaer ...200616751497
evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods.to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ...200716822636
bla(oxa-51)-type beta-lactamase genes are ubiquitous and vary within a strain in acinetobacter baumannii.we investigated the spread of bla(oxa-51)-type beta-lactamase genes in 200 acinetobacter spp. clinical strains isolated in argentina from 1982 to 2005. bla(oxa-51)-type genes were present in all acinetobacter baumannii isolates tested (n=194), whereas they were not detected in two acinetobacter haemolyticus, two genomic species 10 or two acinetobacter lwoffii isolates. the bla(oxa-51)-type alleles varied within a strain and were found in six different a. baumannii pulsed-field gel electrophoresi ...200616844350
acinetobacter lwoffii septicemia associated with a peripheral intravascular catheter. 200616847374
[acinetobacter lwoffii and anitratus]. 200717369976
pseudo-outbreak of acinetobacter lwoffii infection in a tertiary care center in thailand.we describe a pseudo-outbreak of acinetobacter lwoffii infection that was recognized early. the pseudo-outbreak involved 16 patients and occurred 3.5 months after the gns-506 vitek automated system was introduced in the microbiology laboratory. prompt confirmation of incomplete use of the automated system's algorithm as the point source of the misidentified a. lwoffii clinical isolates averted a full outbreak investigation and excess use of infection control resources.200717464933
catheter-related bacteremia and multidrug-resistant acinetobacter lwoffii. 200717479919
acinetobacter lwoffii and lactococcus lactis strains isolated from farm cowsheds possess strong allergy-protective properties.children who grow up in a farming environment show lower levels of atopic sensitization, hay fever, and asthma than children of the same age not living in such an environment. a number of investigations provided good evidence that this is due to an early-life contact with cowsheds, farm animals, and/or consumption of products like raw milk. also, it had been indicated that microorganisms might have an important effect on the development of allergies, and thus the question arose of which farm mic ...200717481709
study of methanol-induced phenotypic changes in a novel strain of acinetobacter lwoffii.a gram-negative bacterial strain designated ls2 isolated from lahaul-spiti valley of north india was shown to produce pink pigment while utilizing methanol as sole source of carbon and energy. interestingly, pigment production was inducible in nature since the organism did not produce any pigment when grown on other carbon sources. based on phenotypic and phylogenetic characterization the non-pigmented methylotroph was identified as a novel strain of acinetobacter lwoffii mtcc 8288 (dq144736). b ...200717572881
characterization of a new catechol branch of the beta-ketoadipate pathway induced for benzoate degradation in acinetobacter lwoffii k24.acinetobacter lwoffii k24 is a known aniline-degrading bacterium. in previous studies, two catechol branches of the beta-ketoadipate pathway were reported to be induced for aniline degradation, and related enzymes (cata(1) and cata(2)) were identified from the aniline-induced proteome of a. lwoffii k24 [s.i. kim, s.h. leem, j.s. choi, y.h. chung, s. kim, y.m. park, y.k. park, y.n. lee, k.s. ha, cloning and characterization of two cata genes in acinetobacter lwoffii k24, j. bacteriol. 179 (1997) ...200717610839
effects of sevoflurane and/or nitrous oxide on bacterial growth in in vitro culture conditions.the aim of this study was to determine the role of sevoflurane and/or nitrous oxide on bacterial growth under conditions in vitro similar to those of clinical practice. we assessed these effects on pseudomonas aeruginosa, acinetobacter lwoffii, and staphylococcus aureus growth. bacterial inoculums were prepared from reference strains in nutritive broth. airtight chambers were filled with bacterial suspensions. each strain was studied with and without exposure to sevoflurane and/or nitrous oxide ...200717680203
chemical composition, antioxidant and antimicrobial properties of the essential oils of three salvia species from turkish flora.essential oils of three different salvia species [salvia aucheri var. aucheri (endemic), salvia aramiensis and salvia pilifera (endemic)] were screened for their possible antioxidant and antimicrobial properties as well as their chemical compositions. according to the gas chromatography (gc)/eims (gas chromatography/electron impact mass spectrum) analysis results; 41 (97.2%), 51 (98.5%) and 83 compounds (98.2%) were identified, respectively. while 1,8-cineole (30.5%), camphor (21.3%) and borneol ...200817936619
bacterial contamination of random-donor platelets in a university hospital in the midwestern region of brazil.bacterial blood contamination was probably the first recognized transfusion-transmitted disease. although the transfusion-associated bacterial sepsis has been known for a long time, it remains an important health problem. at present it is the most frequently reported cause of infectious transfusion-related fatalities. the aims of the study were to determine the prevalence of microbiologic contamination in random-donor platelets (rdps) and to identify the isolated microorganism obtained from a br ...200818005324
[electron microscopic analysis of biofilm on tracheal tubes removed from intubated neonates and the relationship between bilofilm and lower respiratory infection].mechanical ventilation support is a very important method for the salvage of serious patients. however, it can result in the formation of an adherent matrix of bacteria on the surfaces of implanted materials which is termed "biofilm". biofilm is dense bacterial communities attached to a solid surface and surrounded by an exopolysaccharide matrix. one of the most important features of bacterial biofilm is their resistance to antimicrobial agents and host immune system components. as a consequence ...200718021554
binding of nontarget microorganisms from food washes to anti-salmonella and anti-e. coli o157 immunomagnetic beads: most probable composition of background eubacteria.we present herein the composition of bacterial communities occurring in ground chicken and the changes which arise in these populations based upon nonselective partitioning by commercially-available dynal anti-salmonella and anti-e. coli o157 immunomagnetic beads (imb). our enumeration and colony selection protocol was based upon a 6 × 6 drop plate method (n = 18 for each 25-g sub-sampling) using a dilution which resulted in ca. 4-8 colonies per drop. an average of 82 ± 13 colonies were selected ...200818311564
application of pcr-sscp for molecular epidemiological studies on the exposure of farm children to bacteria in environmental dust.the environmental exposure of farm children to microorganisms in dust has become a focus of interest, since microbial exposure on farms has been related to a reduced prevalence of asthma and atopic diseases in children. previous studies almost exclusively focused on the determination of microbial counts using conventional culturing or the determination of microbial compounds i.e. endotoxins. in this study pcr-sscp (single-strand conformation polymorphism) was modified for characterising bacteria ...200818321601
[(3)h]adenine is a suitable radioligand for the labeling of g protein-coupled adenine receptors but shows high affinity to bacterial contaminations in buffer solutions.[(3)h]adenine has previously been used to label the newly discovered g protein-coupled murine adenine receptors. recent reports have questioned the suitability of [(3)h]adenine for adenine receptor binding studies because of curious results, e.g. high specific binding even in the absence of mammalian protein. in this study, we showed that specific [(3)h]adenine binding to various mammalian membrane preparations increased linearly with protein concentration. furthermore, we found that tris-buffer ...200718404448
the efficacy of the inorganic copper-based biocide cuwb50 is compromised by hard water.we sought to explain the unexpected failure of the inorganic copper-based biocide cuwb50 to effectively decontaminate microfibre cleaning cloths that became contaminated with acinetobacter lwoffii.200818422937
isolation and identification of a psychrotrophic acinetobacter sp. cr9 and characterization of its alkaline lipase.forty three psychrotrophic bacteria were isolated from soil samples collected from chandra river in sub-alpine region of western himalaya, india. among these, 11 isolates were found positive for lipase production at low temperature. of 11 isolates, cr9 produced largest zone of clearance on plate assay and was able to produce lipase under wide range of ph. the isolate cr9 was identified as acinetobacter sp. based on morphological and physiochemical characterization and 16s rrna gene sequencing an ...200818506906
[detection methods and changes in metallo-beta-lactamase-producing gram-negative rod isolates at nagasaki university hospital from 1991 to 2005].since plasmid-mediated metallo-beta-lactamase (mbl)-producing pseudimonas aeruginosa was reported in japan, mbl-producing gram-negative rods (gnrs) have emerged worldwide. we developed a way to detect mbl-producing gnrs in routine examination using broth microdilution with the mbl inhibitor sodium mercaptoacetate (sma) in frozen plates. between 1996 and 2005, we evaluated this and other methods, including broth microdilution with another mbl inhibitor dipicolinic acid (dpa) in dry plates, conven ...200818697478
bacterial flora of clinically normal conjunctivae in the domestic duckling.a total of 100 eyes from newly hatched and young domestic ducklings were swabbed and the bacterial flora determined by standard bacteriological culture methods. of the total number of bacteria isolated, 57% were gram negative suggesting that the majority were derived from the intestinal flora. only 2% of the eyes sampled showed no bacterial growth. the bacterial species most commonly cultured were escherichia coli, staphylococcus epidermidis, acinetobacter anitratus, klebsiella pneumoniae, non-h ...198518766899
effect of bacterial interference on biofilm development by legionella pneumophila.in the ecology of legionella pneumophila a crucial role may be played by its relationship with the natural flora; thus we investigated the interactions between legionella and other aquatic bacteria, particularly within biofilms. among 80 aquatic bacteria screened for the production of bacteriocin-like substances (blss), 66.2% of them were active against l. pneumophila. the possible effect of some of these aquatic bacteria on the development and stability of l. pneumophila biofilms was studied. p ...200818769851
structural and immunochemical analysis of the lipopolysaccharide from acinetobacter lwoffii f78 located outside chlamydiaceae with a chlamydia-specific lipopolysaccharide epitope.chemical analyses, nmr spectroscopy, and mass spectrometry were used to elucidate the structure of the rough lipopolysaccharide (lps) isolated from acinetobacter lwoffii f78. as a prominent feature, the core region of this lps contained the disaccharide alpha-kdo-(2-->8)-alpha-kdo (kdo=3-deoxy-d-d-manno-oct-2-ulopyranosonic acid), which so far has been identified only in chlamydial lps. in serological investigations, the anti-chlamydial lps monoclonal antibody s25-2, which is specific for the ep ...200818850609
carbon source-dependent modulation of nadp-glutamate dehydrogenases in isophthalate-degrading pseudomonas aeruginosa strain pp4, pseudomonas strain ppd and acinetobacter lwoffii strain isp4.acinetobacter lwoffii strain isp4 metabolizes isophthalate rapidly compared with pseudomonas aeruginosa strain pp4 and pseudomonas strain ppd. isophthalate has been reported to be a potent competitive inhibitor of glutamate dehydrogenase (gdh). exogenous supplementation of isophthalate with glutamate or alpha-ketoglutarate at 1 mm concentration caused strains pp4 and ppd to grow faster than in the presence of isophthalate alone; however, no such effect was observed in strain isp4. when grown on ...200818957586
in vitro activity of tigecycline as a therapeutic option against multidrug-resistant acinetobacter spp.the study was performed to detect the in vitro activity of tigecycline in multidrug-resistant acinetobacter isolates from patients in hacettepe university adult hospital, turkey. the microorganisms were isolated from clinical specimens of patients with respiratory and bloodstream infections. thirty (66.7%) of the 45 inpatients were in icus. in vitro activity of imipenem, meropenem, ceftazidime, ciprofloxacin and aztreonam in 124 acinetobacter species isolated was evaluated by microdilution test. ...200819123310
galleria mellonella as a model system to study acinetobacter baumannii pathogenesis and therapeutics.nonmammalian model systems of infection such as galleria mellonella (caterpillars of the greater wax moth) have significant logistical and ethical advantages over mammalian models. in this study, we utilize g. mellonella caterpillars to study host-pathogen interactions with the gram-negative organism acinetobacter baumannii and determine the utility of this infection model to study antibacterial efficacy. after infecting g. mellonella caterpillars with a reference a. baumannii strain, we observe ...200919332683
prevalence and antimicrobial susceptibility of gram-negative bacteria isolated from bovine mastitis between 2003 and 2008 in korea.the objective of this study was to assess trends in the prevalence and distribution of gram-negative bacteria isolated from bovine mastitis and their antimicrobial susceptibilities during a 6-yr period between 2003 and 2008 in korea. escherichia coli, pseudomonas fluorescens, klebsiella pneumoniae, enterobacter cloacae, acinetobacter lwoffi/junii, pseudomonas aeruginosa, and serratia marcescens were the most commonly observed pathogens during this period. generally, gram-negative bacteria showed ...200919389959
detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in hungary.the drinking water distribution system of a hospital was investigated using standard cultivation techniques, taxon-specific pcrs targeting pathogenic bacteria, denaturing gradient gel electrophoresis, cloning and sequencing. the results obtained verify the higher sensitivity of pcr compared to cultivation for detecting legionella and pseudomonas aeruginosa. moreover, several other opportunistic pathogenic bacteria, such as escherichia albertii, acinetobacter lwoffi and corynebacterium tuberculos ...201019519854
determination of in vitro antioxidative and antimicrobial properties and total phenolic contents of ziziphora clinopodioides, cyclotrichium niveum, and mentha longifolia ssp. typhoides var. typhoides.the aerial parts of ziziphora clinopodioides, cyclotrichium niveum, and mentha longifolia ssp. typhoides var. typhoides were screened for their possible antioxidant and antimicrobial activities in addition to their penolic contents. antioxidant activity was employed by two complementary test systems: 2,2'-diphenyl-1-picrylhydrazyl free radical scavenging and beta-carotene/linoleic acid. in the first case, z. clinopodioides was superior to the other species with a 50% inhibitory concentration val ...200919627221
acinetobacter lwoffii: bacteremia associated with acute gastroenteritis.acinetobacter lwoffii is a non-fermentative aerobic gram-negative bacillus that is seen as a normal flora of the oropharynx and skin in approximately 25% of the healthy individuals. due to its ubiquitous nature, it is a potential opportunistic pathogen in patients with impaired immune systems, and it has been identified as a cause of nosocomial infections like septicemia, pneumonia, meningitis, urinary tract infections, skin and wound infections. to our knowledge, this is the first case reported ...200919747669
endemic and epidemic acinetobacter species in a university hospital: an 8-year survey.the prevalence of the currently known acinetobacter species and related trends of antimicrobial resistance in a dutch university hospital were studied. between 1999 and 2006, acinetobacter isolates from clinical samples were collected prospectively. isolates were analyzed by amplified fragment length polymorphism fingerprinting. for species identification, a profile similarity cutoff level of 50% was used, and for strain identification, a cutoff level of 90% was used. susceptibility for antimicr ...200919794057
[antimicrobial resistance and existence of metallo-beta-lactamase in acinetobacter species isolated from adult patients].acinetobacter spp. are the frequent causes of nosocomial infections which are difficult to treat due to multidrug resistance. the aim of this study was to determine the antibiotic susceptibilities and the presence of metallo-beta-lactamases in acinetobacter spp. isolated from patients admitted to hacettepe university adult hospital. a total of 124 acinetobacter spp. isolates were included in the study. antibiotic susceptibilities against imipenem (imp), meropenem (mer), ceftazidime (caz), ciprof ...200919795613
the allergy-protective properties of acinetobacter lwoffii f78 are imparted by its lipopolysaccharide.an increasing number of epidemiological studies show that exposure to farming environment during early childhood strongly influences the development of allergic reactions later in life ('hygiene hypothesis'). also, it had been shown that certain bacteria from this environment may have allergy-protective properties. in the present study, we further characterized one of these bacteria, namely acinetobacter lwoffii f78, with regard to the bacteria-induced signaling and possible mechanisms of allerg ...201019909295
bypassing isophthalate inhibition by modulating glutamate dehydrogenase (gdh): purification and kinetic characterization of nadp-gdhs from isophthalate-degrading pseudomonas aeruginosa strain pp4 and acinetobacter lwoffii strain isp4.pseudomonas aeruginosa strain pp4 and acinetobacter lwoffii strain isp4 metabolize isophthalate as a sole source of carbon and energy. isophthalate is known to be a competitive inhibitor of glutamate dehydrogenase (gdh), which is involved in c and n metabolism. strain pp4 showed carbon source-dependent modulation of nadp-gdh; gdh(i) was produced when cells were grown on isophthalate, while gdh(ii) was produced when cells were grown on glucose. strain isp4 produced a single form of nadp-gdh, gdh( ...201019933355
maternal tlr signaling is required for prenatal asthma protection by the nonpathogenic microbe acinetobacter lwoffii f78.the pre- and postnatal environment may represent a window of opportunity for allergy and asthma prevention, and the hygiene hypothesis implies that microbial agents may play an important role in this regard. using the cowshed-derived bacterium acinetobacter lwoffii f78 together with a mouse model of experimental allergic airway inflammation, this study investigated the hygiene hypothesis, maternal (prenatal) microbial exposure, and the involvement of toll-like receptor (tlr) signaling in prenata ...200919995952
[not available]. 194720268392
structure of the o-antigen of acinetobacter lwoffii ek30a; identification of d-homoserine, a novel non-sugar component of bacterial polysaccharides.we established a peculiar structure of the o-specific polysaccharide (o-antigen) of a psychrotrophic strain of acinetobacter lwoffii, ek30a, isolated from a 1.6-1.8 million-year-old siberian permafrost subsoil sediment sample. the polysaccharide was released by mild acid degradation of the lipopolysaccharide and studied using chemical analyses, smith degradation, (1)h and (13)c nmr spectroscopy and mass spectrometry. it was found to contain d-homoserine, which is n-linked to 4-amino-4,6-dideoxy- ...201020539880
fulminant community-acquired pneumonia probably caused by acinetobacter lwoffii.we describe a case of fulminant community-acquired pneumonia probably caused by acinetobacter lwoffii. a 63-year-old woman was admitted with an acute respiratory illness and consolidation in the right upper lobe. she deteriorated rapidly despite antibiotic therapy, and required ventilatory support. the patient died of multiple organ failure and intractable shock, 7 days after admission to hospital; her disease was probably caused by a. lwoffii.201020546186
the effect of carbon source on microbial community structure and cr(vi) reduction rate.in the present work, the effect of the carbon source on microbial community structure in batch cultures derived from industrial sludge and hexavalent chromium reduction was studied. experiments in aerobic batch reactors were carried out by amending industrial sludge with two different carbon sources: sodium acetate and sucrose. in each of the experiments performed, four different initial cr(vi) concentrations of: 6, 13, 30 and 115 mg/l were tested. the change of carbon source in the batch reacto ...201020552669
bordetella holmesii bacteremia in asplenic children: report of four cases initially misidentified as acinetobacter lwoffii.bordetella holmesii is a fastidious gram-negative rod that was initially identified in 1995. it causes bacteremia, predominantly among patients with anatomical or functional asplenia. we report four cases of b. holmesii bacteremia in asplenic children occurring within the last 4 years. in all cases, b. holmesii was misidentified by an automated system as acinetobacter lwoffii.201020668129
structure of the o-polysaccharide of acinetobacter sp. vs-15 and acinetobacter lwoffii ek67.an o-polysaccharide was released by mild acid degradation of the lipopolysaccharide of acinetobacter sp. vs-15 and studied by chemical methods along with (1)h and (13)c nmr spectroscopy, including 2d (1)h,(1)h cosy, tocsy, roesy, (1)h,(13)c hsqc and hmbc experiments. the following structure of the pentasaccharide repeating unit of the polysaccharide was established: [formula: see text]. the o-polysaccharide of acinetobacter lwoffii ek67 was found to have the same structure.201020732679
oxa-134, a naturally occurring carbapenem-hydrolyzing class d beta-lactamase from acinetobacter lwoffii.acinetobacter lwoffii, a species whose natural habitat is the human skin, intrinsically possesses a chromosomal gene encoding a carbapenem-hydrolyzing class d β-lactamase, oxa-134. this species may therefore constitute a reservoir for carbapenemase genes that may spread among other acinetobacter species.201020837764
in vivo selection of a missense mutation in ader and conversion of the novel blaoxa-164 gene into blaoxa-58 in carbapenem-resistant acinetobacter baumannii isolates from a hospitalized patient.the mechanism of stepwise acquired multidrug resistance in acinetobacter baumannii isolates from a hospitalized patient was investigated. thirteen consecutive multidrug-resistant isolates were recovered from the same patient over a 2-month period. the vitek 2 system identified the isolates as meropenem-sensitive acinetobacter lwoffii; however, molecular identification showed that the isolates were a. baumannii. etest revealed that the isolates were meropenem resistant. the presence of oxacillina ...201020921306
Displaying items 101 - 200 of 282