Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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effects of lactulose supplementation on the growth of bifidobacteria and biotransformation of isoflavone glycosides to isoflavone aglycones in soymilk. | bifidobacterium animalis subsp. lactis bb12 and b. longum 20099 were used to hydrolyze isoflavone glycosides (ig) to biologically active forms, which are isoflavone aglycones (ia), in soymilk (sm) prepared from soy protein isolate (spi) and sm supplemented with 0.5% (w/v) of lactulose (sml). supplementation of lactulose significantly ( p < 0.05) enhanced the viable counts of b. animalis subsp. lactis bb12 and b. longum 20099 up to 2.34 and 2.15 log cfu/ml, respectively. bifidobacterium animalis ... | 2008 | 18500812 |
effect of the ingestion of a symbiotic yogurt on the bowel habits of women with functional constipation. | functional constipation is a prevalent problem within the western population. there is evidence supporting the fact that the inclusion of pre and probiotics in the diet can favorably modify the intestinal function. the present study evaluates the effect of the consumption of activia, a yogurt containing 10(8) ufc/g of bifidobacterium animalis (dn-173 010) and fructoligosaccharide, in women between the ages of 18 and 55 with and without functional constipation (rome ii criteria). | 2008 | 18533353 |
effect of fructo-oligosaccharides and different doses of bifidobacterium animalis in a weaning diet on bacterial translocation and toll-like receptor gene expression in pigs. | our aim was to study the possible synergic action of one prebiotic with increasing dietary doses of a probiotic strain of bifidobacterium animalis on the translocation of bifidobacteria and on toll-like receptor (tlr) gene expression in different organs of weaned piglets. | 2008 | 18562167 |
effect of inulin on the human gut microbiota: stimulation of bifidobacterium adolescentis and faecalibacterium prausnitzii. | prebiotics are food ingredients that improve health by modulating the colonic microbiota. the bifidogenic effect of the prebiotic inulin is well established; however, it remains unclear which species of bifidobacterium are stimulated in vivo and whether bacterial groups other than lactic acid bacteria are affected by inulin consumption. changes in the faecal microbiota composition were examined by real-time pcr in twelve human volunteers after ingestion of inulin (10 g/d) for a 16-d period in co ... | 2009 | 18590586 |
analysis of tetracycline resistance tet(w) genes and their flanking sequences in intestinal bifidobacterium species. | the tet(w) gene provides tetracycline resistance to a wide range of anaerobic intestinal and ruminal bacteria, but little is known about the molecular organization of the tet(w) gene. the aim of this study was to gain new insights into the molecular organization of the tet(w) gene in bifidobacteria strains from humans. | 2008 | 18614524 |
early administration of probiotics alters bacterial colonization and limits diet-induced gut dysfunction and severity of necrotizing enterocolitis in preterm pigs. | following preterm birth, bacterial colonization and enteral formula feeding predispose neonates to gut dysfunction and necrotizing enterocolitis (nec), a serious gastrointestinal inflammatory disease. we hypothesized that administration of probiotics would beneficially influence early bacterial colonization, thereby reducing the susceptibility to formula-induced gut atrophy, dysfunction, and nec. caesarean-delivered preterm pigs were provided total parenteral nutrition (1.5 d) followed by entera ... | 2008 | 18641188 |
detection of bifidobacterium animalis subsp. lactis (bb12) in the intestine after feeding of sows and their piglets. | a real-time pcr method has been developed to distinguish bifidobacterium animalis subspecies in the gastrointestinal tracts of pigs. identification of a highly conserved single-copy tuf gene encoding the elongation factor tu involved in bacterial protein biosynthesis was used as a marker to differentiate homologous bifidobacterium animalis subsp. lactis (strain bb12) from bifidobacterium animalis subsp. animalis, as well as bifidobacterium suis, bifidobacterium breve, bifidobacterium longum, sev ... | 2008 | 18689506 |
a differential effect of 2 probiotics in the prevention of eczema and atopy: a double-blind, randomized, placebo-controlled trial. | the role of probiotics in prevention of allergic disease is still not clearly established, although early reports suggested lactobacillus gg halved the risk of eczema at 2 years. | 2008 | 18762327 |
transport of glucose by bifidobacterium animalis subsp. lactis occurs via facilitated diffusion. | two strains of bifidobacterium animalis subsp. lactis were indistinguishable by several nucleic acid-based techniques; however, the type strain dsmz 10140 was glucose utilization positive, while rb 4825, an industrially employed strain, was unable to grow rapidly on glucose as the principal carbon source. this difference was attributed to the presence of a low-affinity facilitated-diffusion glucose transporter identified in dsmz 10140 but lacking in rb 4825. uptake of d-[u-(14)c]glucose in dsmz ... | 2008 | 18791026 |
genome sequence of the probiotic bacterium bifidobacterium animalis subsp. lactis ad011. | bifidobacterium animalis subsp. lactis is a probiotic bacterium that naturally inhabits the guts of most mammals, including humans. here we report the complete genome sequence of b. animalis subsp. lactis ad011 that was isolated from an infant fecal sample. biological functions encoded in a single circular chromosome of 1,933,695 bp, smallest among the completely sequenced bifidobacterial genomes, are suggestive of their probiotic functions, such as utilization of bifidogenic factors and a varie ... | 2009 | 19011029 |
in vitro kinetic analysis of fermentation of prebiotic inulin-type fructans by bifidobacterium species reveals four different phenotypes. | kinetic analyses of bacterial growth, carbohydrate consumption, and metabolite production of 18 bifidobacterium strains grown on fructose, oligofructose, or inulin were performed. a principal component analysis of the data sets, expanded with the results of a genetic screen concerning the presence of a beta-fructofuranosidase gene previously encountered in bifidobacterium animalis subsp. lactis dsm 10140(t), revealed the existence of four clusters among the bifidobacteria tested. strains belongi ... | 2009 | 19011052 |
effects of bifidobacterium animalis administered during lactation on allergic and autoimmune responses in rodents. | probiotics are promoted as being beneficial to health and positive effects on the immune system have been reported. beneficial immune effects have been attributed to several mechanisms, including stimulating t helper 1 (th1) immunity. to explore the effects of the probiotic bifidobacterium animalis on th1- and th2-mediated immune responses, two different animal models representing either th1- or th2-mediated immune responses were used: a rat model for experimental autoimmune encephalomyelitis (e ... | 2008 | 19037925 |
bile affects the synthesis of exopolysaccharides by bifidobacterium animalis. | by using cryo-scanning electron microscopy and quantification with lectin-conjugated probes, we have detected the production of exopolysaccharides (eps) in bifidobacterium animalis subsp. lactis in the presence of bile. in addition, the expression of gtf01207, which codifies a putative priming glycosyltransferase involved in eps synthesis, was induced by bile. | 2009 | 19088310 |
comparative effects of a high-amylose starch and a fructooligosaccharide on fecal bifidobacteria numbers and short-chain fatty acids in pigs fed bifidobacterium animalis. | pigs were fed a freeze-dried probiotic (bifidobacterium animalis cscc 1941) plus a high-amylose maize starch (hams) and a fructooligosaccharide (fos) separately or together. fecal output and total and individual major short-chain fatty acid (scfa) concentrations and excretion were higher and ph was lower with hams than with fos relative to when they were fed a low-amylose maize starch (lams; control). fecal bifidobacteria numbers and total excretion were equally higher during feeding of fos or h ... | 2009 | 19089616 |
exploring the diversity of the bifidobacterial population in the human intestinal tract. | although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. we performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rrna gene sequences (16s rrna gene and internally transcribed spacer [its] 16s-23s spacer sequences) of isolated colonies. a total of 900 isolate ... | 2009 | 19168652 |
reduction of salivary mutans streptococci in orthodontic patients during daily consumption of yoghurt containing probiotic bacteria. | previous studies have suggested that probiotic supplements in dairy products may affect the oral microbial ecology, but the effect in orthodontic patients has not previously been reported. the aim of the present study was to examine whether short-term consumption of fruit yogurt containing probiotic bifidobacteria would affect the levels of salivary mutans streptococci and lactobacilli in patients with fixed orthodontic appliances. a double-blind, randomized crossover study was performed and 24 ... | 2009 | 19193706 |
bifidobacterium animalis causes extensive duodenitis and mild colonic inflammation in monoassociated interleukin-10-deficient mice. | we recently showed that bifidobacterium animalis is more prevalent within the colons of interleukin (il)-10-deficient (-/-) mice than in wildtype (wt) animals colonized with the same specific pathogen-free (spf) fecal contents. here we tested the ability of this organism to cause t-cell-mediated intestinal inflammation by introducing it into germ-free (gf) il-10-/- mice. | 2009 | 19235917 |
probiotics and functional gastrointestinal disorders in children. | chronic constipation is one of the most frequent complaints in childhood. although there is evidence that gastrointestinal flora is important in gut motility, there is little evidence that gut flora is abnormal in constipation. lactobacilli and bifidobacteria increase stool frequency and decrease consistency in normal individuals. but, according to several reviews, the evidence of probiotics for efficacy in constipation is limited. fiber supplements, lactose-free diets, and lactobacillus supplem ... | 2009 | 19300120 |
bile-inducible efflux transporter from bifidobacterium longum ncc2705, conferring bile resistance. | bifidobacteria are normal inhabitants of the human gut. some strains of this genus are considered health promoting or probiotic, being included in numerous food products. in order to exert their health benefits, these bacteria must overcome biological barriers, including bile salts, to colonize and survive in specific parts of the intestinal tract. the role of multidrug resistance (mdr) transporters in bile resistance of probiotic bacteria and the effect of bile on probiotic gene expression are ... | 2009 | 19304838 |
tolerance and safety of lactobacillus paracasei ssp. paracasei in combination with bifidobacterium animalis ssp. lactis in a prebiotic-containing infant formula: a randomised controlled trial. | the addition of probiotics to infant formula has been shown to be an efficient way to increase the number of beneficial bacteria in the intestine in order to promote a gut flora resembling that of breast-fed infants. the objective of the present study was to evaluate the safety and tolerance of a combination of two probiotic strains in early infancy. a group of 126 newborns were randomised to receive a prebiotic-containing starter formula supplemented with lactobacillus paracasei ssp. paracasei ... | 2009 | 19331702 |
native wheat, potato and pea starches and their physically modified preparations tested in vitro as the substrates for selected bifidobacterium strains. | the wheat, potato and pea starches subjected to physical modification were studied in vitro as a substrate for selected bifidobacterium strains (bifidobacterium breve kn14, bifidobacterium animalis ks20a1). the effects of these substrates on bifidobacterial growth, acidifying activity, metabolic end-products and changes in starch microstructure were assayed after 24-h fermentation. physically modified starch preparations were better utilized substrates for growth of bifidobacterium monocultures, ... | 2009 | 19353367 |
comparison of the complete genome sequences of bifidobacterium animalis subsp. lactis dsm 10140 and bl-04. | bifidobacteria are important members of the human gut flora, especially in infants. comparative genomic analysis of two bifidobacterium animalis subsp. lactis strains revealed evolution by internal deletion of consecutive spacer-repeat units within a novel clustered regularly interspaced short palindromic repeat locus, which represented the largest differential content between the two genomes. additionally, 47 single nucleotide polymorphisms were identified, consisting primarily of nonsynonymous ... | 2009 | 19376856 |
enumeration of the contaminating bacterial microbiota in unfermented pasteurized milks enriched with probiotic bacteria. | pasteurized and unfermented milks supplemented with probiotic bacteria are appearing on the market. it then becomes a challenge to ascertain the undesirable contamination microbiota in the presence of a largely superior population of probiotic bacteria. a method to enumerate the contaminating microbial microbiota in such probiotic-enriched milks was developed. the probiotic cultures, lactobacillus rhamnosus lb-immuni-t and bifidobacterium animalis subsp. lactis bb-12(r), were added to a pasteuri ... | 2009 | 19396241 |
[screening and identification of probiotic bifidobacterium from mongolian children]. | bifidobacterium has important physiological activity in gastrointestinal tract of human and animals, and the tolerability of acid, intestinal juice and oxgall bile salt are the key factors that influence the function of living bacteria preparation. in this study, feces of 12 healthy mongolian children were selected to isolate bifidobacterium with probiotic properties. | 2009 | 19445177 |
prevention of tnbs-induced colitis by different lactobacillus and bifidobacterium strains is associated with an expansion of gammadeltat and regulatory t cells of intestinal intraepithelial lymphocytes. | probiotics may protect against inflammatory bowel disease through regulation of lamina propria lymphocytes (lpls) function. data are lacking on possible involvement of intraepithelial lymphocytes (iels). the aim of this study was to investigate whether different probiotic mixtures prevented gut inflammatory disease and the role of both iels and lpls. | 2009 | 19504616 |
portrait of a canine probiotic bifidobacterium--from gut to gut. | the gastrointestinal environment is a complex interactive system involving the host, ingested dietary components, and numerous microbial species. we hypothesized that isolation and screening of lactobacilli and bifidobacteria adherent to healthy canine gastrointestinal tissue would yield strains with commensal activity in canines. the aims of this study were (1) to isolate a bank of commensal organisms from the canine gastrointestinal tract; (2) to screen these novel microbial isolates for poten ... | 2009 | 19523775 |
comparative study of bifidobacterium animalis, escherichia coli, lactobacillus casei and saccharomyces boulardii probiotic properties. | the present work investigates some probiotic properties of four different microorganisms (bifidobacterium animalis var. lactis bb-12, escherichia coli emo, lactobacillus casei and saccharomyces boulardii). in vitro and in vivo tests were carried out to compare cell wall hydrophobicity, production of antagonistic substances, survival capacity in the gastrointestinal tract of germ-free mice without pathological consequence, and immune modulation by stimulation of küpffer cells, intestinal siga and ... | 2009 | 19526225 |
quantification of live and dead probiotic bacteria in lyophilised product by real-time pcr and by flow cytometry. | the basic requirement for probiotic bacteria to be able to exert expected positive effects is to be alive; therefore, appropriate quantification methods are crucial. due to disadvantages of conventional microbiological methods, the bacterial quantification based on the nucleic acid detection is increasingly used. the objective of this study was to evaluate the possibility to use propidium monoazide (pma) in combination with real-time polymerase chain reaction (pcr) method or live/dead baclight v ... | 2009 | 19529931 |
probiotic effects on cold and influenza-like symptom incidence and duration in children. | probiotic consumption effects on cold and influenza-like symptom incidence and duration were evaluated in healthy children during the winter season. | 2009 | 19651563 |
dynamics of fecal microbiota in hospitalized elderly fed probiotic lkm512 yogurt. | the comprehensive dynamics of intestinal microbiota including uncultured bacteria in response to probiotic consumption have not been well studied. the aims of this study were twofold: firstly to analyze the impact on intestinal microbiota of yogurt fermented by bifidobacterium animalis subsp. lactis lkm512, lactobacillus delbrueckii subsp. bulgaricus lkm1759, and streptococcus thermophilus lkm1742 (lkm512 yogurt) and placebo fermented by these lactic acid bacterial strains without lkm512; and se ... | 2009 | 19659926 |
production of exopolysaccharides by lactobacillus and bifidobacterium strains of human origin, and metabolic activity of the producing bacteria in milk. | this work reports on the physicochemical characterization of 21 exopolysaccharides (eps) produced by lactobacillus and bifidobacterium strains isolated from human intestinal microbiota, as well as the growth and metabolic activity of the eps-producing strains in milk. the strains belong to the species lactobacillus casei, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus vaginalis, bifidobacterium animalis, bifidobacterium longum, and bifidobacterium pseudocatenulatum. the molar ma ... | 2009 | 19700676 |
the effect of pomegranate (punica granatum l.) byproducts and ellagitannins on the growth of human gut bacteria. | the consumption of pomegranate products leads to a significant accumulation of ellagitannins in the large intestines, where they interact with complex gut microflora. this study investigated the effect of pomegranate tannin constituents on the growth of various species of human gut bacteria. our results showed that pomegranate byproducts and punicalagins inhibited the growth of pathogenic clostridia and staphyloccocus aureus. probiotic lactobacilli and bifidobacteria were generally not affected ... | 2009 | 19705832 |
exopolysaccharides produced by bifidobacterium longum ipla e44 and bifidobacterium animalis subsp. lactis ipla r1 modify the composition and metabolic activity of human faecal microbiota in ph-controlled batch cultures. | exopolysaccharides (eps) isolated from two bifidobacterium strains, one of human intestinal origin (bifidobacterium longum subsp. longum ipla e44) and the other from dairy origin (bifidobacterium animalis subsp. lactis ipla r1), were subjected to in vitro chemically simulated gastrointestinal digestion, which showed the absence of degradation of both polymers in these conditions. polymers were then used as carbon sources in ph-controlled faecal batch cultures and compared with the non-prebiotic ... | 2009 | 19735956 |
strain-specific genotyping of bifidobacterium animalis subsp. lactis by using single-nucleotide polymorphisms, insertions, and deletions. | several probiotic strains of bifidobacterium animalis subsp. lactis are widely supplemented into food products and dietary supplements due to their documented health benefits and ability to survive within the mammalian gastrointestinal tract and acidified dairy products. the strain specificity of these characteristics demands techniques with high discriminatory power to differentiate among strains. however, to date, molecular approaches, such as pulsed-field gel electrophoresis and randomly ampl ... | 2009 | 19801460 |
comparison of in vitro models to study bacterial adhesion to the intestinal epithelium. | to evaluate the adhesion ability of intestinal bacteria to different in vitro models of intestinal epithelia, and to estimate the suitability of these models and the type of interactions involved. | 2009 | 19843211 |
clinical benefits of probiotic canine-derived bifidobacterium animalis strain ahc7 in dogs with acute idiopathic diarrhea. | this study evaluated the effect of supplementation with canine-derived probiotic bifidobacterium animalis strain ahc7 (lams prostora, procter & gamble pet care) on the resolution rate of acute idiopathic diarrhea in dogs randomly assigned to receive a placebo (n=18) or the probiotic (n=13). nutritional management with the probiotic fed at 2 x 10(10) cfu/day significantly reduced the time to resolution (3.9 +/- 2.3 versus 6.6 +/- 2.7 days; p < .01) and reduced the percentage of dogs that were adm ... | 2009 | 20037966 |
species delineation and clonal diversity in four bifidobacterium species as revealed by multilocus sequencing. | the genus bifidobacterium comprises several species that are important contributors to the gut microbiome, with some strains having beneficial health effects. understanding the evolutionary emergence of advantageous biological properties requires knowledge of the genetic diversity and clonal structure of species. we sequenced seven housekeeping genes in 119 bifidobacterium strains of bifidobacterium animalis, bifidobacterium bifidum, bifidobacterium breve and bifidobacterium longum. phylogenetic ... | 2010 | 20060895 |
distinct bifidobacterium strains drive different immune responses in vitro. | in this work we evaluated the specific immune activation properties of different bifidobacterium strains, some of the most relevant intestinal microorganisms. to this end, we examined the in vitro effect of 12 bifidobacterium strains belonging to 4 different species, bifidobacterium longum, bifidobacterium breve, bifidobacterium bifidum and bifidobacterium animalis subsp. lactis, on the maturation pattern of human monocyte-derived dendritic cells (dcs), as well as in their ability to induce cyto ... | 2010 | 20071048 |
dietary supplementation with lactobacilli and bifidobacteria is well tolerated and not associated with adverse events during late pregnancy and early infancy. | lactic acid bacteria and bifidobacteria are increasingly being administered to pregnant women and infants with the intention of improving health. although these organisms have a long record of safe use, it is important to identify any adverse effects in potentially vulnerable populations. in a randomized, double-blinded, placebo-controlled trial, we evaluated the safety of a bacterial dietary supplement for the prevention of atopy in infants. two strains of lactobacilli (lactobacillus salivarius ... | 2010 | 20089774 |
antibiotic susceptibility of bifidobacterial strains distributed in the japanese market. | the aim of the present study was to analyze the antibiotic susceptibility of bifidobacterial strains distributed in the japanese market. a total of 23 strains, including probiotic isolates from foods, supplements, pharmaceuticals and reference strains of each species (or subspecies), were tested for susceptibility to 15 antibiotics by the broth microdilution method and examined for the presence of possible resistant determinants. the strains were susceptible overall to chloramphenicol, ampicilli ... | 2010 | 20139616 |
dnak from bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts. | bifidobacterium animalis subsp. lactis lives in the gastrointestinal tract of most mammals, including humans. recently, for the probiotic strain b. animalis subsp. lactis bi07, a dose-dependent plasminogen-binding activity was demonstrated and five putative plasminogen-binding proteins were identified. here we investigated the role of surface dnak as a b. animalis subsp. lactis bi07 plasminogen receptor. dnak was visualized on the bacterial cell surface by transmission electron microscopy. the h ... | 2010 | 20167618 |
complete genome sequence of bifidobacterium animalis subsp. lactis bb-12, a widely consumed probiotic strain. | bifidobacterium animalis subsp. lactis bb-12 is a commercially available probiotic strain used throughout the world in a variety of functional foods and dietary supplements. the benefits of bb-12 have been documented in a number of independent clinical trials. determination of the complete genome sequence reveals a single circular chromosome of 1,942,198 bp with 1,642 predicted protein-encoding genes, 4 rrna operons, and 52 trna genes. knowledge of this sequence will lead to insight into the spe ... | 2010 | 20190051 |
the study to investigate the potential benefits of probiotics in yogurt, a patient-oriented, double-blind, cluster-randomised, placebo-controlled, clinical trial. | probiotic functional foods are widely advertised to consumers primarily based on probiotic supplements. | 2010 | 20216564 |
genetic basis of tetracycline resistance in bifidobacterium animalis subsp. lactis. | all strains of bifidobacterium animalis subsp. lactis described to date show medium level resistance to tetracycline. screening of 26 strains from a variety of sources revealed the presence of tet(w) in all isolates. a transposase gene upstream of tet(w) was found in all strains, and both genes were cotranscribed in strain iplaic4. mutants with increased tetracycline resistance as well as tetracycline-sensitive mutants of iplaic4 were isolated and genetically characterized. the native tet(w) gen ... | 2010 | 20348299 |
[probiotic properties of industrial strains of lactobacilli and bifidobacteria]. | lactic acid bacteria and bifidobacteria were isolated from the samples of dry microbial substance "vivolac" and identified. the lactic acid bacteria were identified as lactobacillus acidophilus. l. delbrueckii subsp. bulgaricus, l. paracasei. bifidobacteria belonged to the species bifidobacterium animalis. manifestation of biological activity of all studied strains was investigated. it was shown, that adhesive activity of the studied strains depended on the genus of the strain: lactobacilli mani ... | 2010 | 20364710 |
effects of synbiotic-based bifidobacterium animalis in female rats experimentally infected with toxoplasma gondii. | the aim of this study was to assess the effects of a synbiotic composed of bifidobacterium animalis and fructooligosaccharides on female rats infected with toxoplasma gondii. female wistar rats, treated or not with dexamethasone, were daily supplemented with synbiotics for 21 days. after 15 days of supplementation, the rats were orally infected with 10(4)t. gondii bradyzoites. blood samples were collected to measure the levels of ifn-γ, il-10 and t. gondii antibodies. all synbiotic-supplemented ... | 2011 | 20409588 |
isolation and characterisation of an oxygen, acid and bile resistant bifidobacterium animalis subsp. lactis qq08. | currently, bifidobacteria are recognised as one of the most important bacteria used as probiotics, which promote human health. however, their commercial application has been limited by their anaerobic nature. the purpose of this study was to select an oxygen, acid and bile resistant strain of bifidobacterium for use as a new probiotic. | 2010 | 20474053 |
complete genome sequence of probiotic bifidobacterium animalis subsp. lactis strain v9. | bifidobacterium animalis subsp. lactis strain v9 is a chinese commercial bifidobacteria with several probiotic functions. it was isolated from a healthy mongolian child in china. we present here the complete genome sequence of v9 and compare it to 3 other published genome sequences of b. animalis subsp. lactis strains. the result indicates the lack of polymorphism among strains of this subspecies from different continents. | 2010 | 20511504 |
effect of bifidobacterium animalis subsp lactis supplementation in preterm infants: a systematic review of randomized controlled trials. | to systematically evaluate and update evidence on the efficacy and safety of bifidobacterium animalis subsp lactis cncm i-3446 supplementation in preterm infants. | 2010 | 20543719 |
probiotics in pregnant women to prevent allergic disease: a randomized, double-blind trial. | previous reports have suggested that certain probiotics given to mothers and children at risk of atopy halves the incidence of atopic dermatitis (ad) at 2 years of age. | 2010 | 20545688 |
impact of probiotic supplementation on mortality of induced 1,2-dimethylhydrazine carcinogenesis in a mouse model. | probiotic bacterial strains have been increasingly used in clinical practice as many health benefits result from their use. however, severe side effects such as bacteremia and fungemia have been reported in inmunocompromised patients and those with chronic disease. | 2010 | 20579593 |
antioxidant activity in vitro of the selenium-contained protein from the se-enriched bifidobacterium animalis 01. | several studies indicated that bifidobacteria possessed strong antioxidant activity. in present study, the antioxidant activities of bifidobacterium animalis 01 proteins were evaluated using six assays, namely, linoleic acid preoxidation assay, erythrocyte hemolysis assay, 1,1-diphenyl-2-picrylhydrazyl (dpph) assay, reducing power assay, hydroxyl (.oh) and superoxide radicals (.o(2)(-)) assays, in which the last two assays were measured by electron spin resonance (esr). there were two kinds of b ... | 2010 | 20601030 |
oxalate-degrading activity in bifidobacterium animalis subsp. lactis: impact of acidic conditions on the transcriptional levels of the oxalyl coenzyme a (coa) decarboxylase and formyl-coa transferase genes. | oxalic acid occurs extensively in nature and plays diverse roles, especially in pathological processes. due to its highly oxidizing effects, hyperabsorption or abnormal synthesis of oxalate can cause serious acute disorders in mammals and can be lethal in extreme cases. intestinal oxalate-degrading bacteria could therefore be pivotal in maintaining oxalate homeostasis and reducing the risk of kidney stone development. in this study, the oxalate-degrading activities of 14 bifidobacterial strains ... | 2010 | 20601517 |
survival of bifidobacteria administered to calves. | twenty-five bifidobacteria were isolated from feces of calves. isolates were identified, and their functional properties and antimicrobial activity were determined. from 10 strains with suitable properties rifampicin-resistant mutants (rrbs) were prepared and mixture of rrbs was administered to 2-d-old calves. these strains were identified by sequencing as bifidobacterium animalis ssp. animalis (6 strains), b. thermophilum (2 strains), b. choerinum (1 strain) and b. longum ssp. suis (1 strain). ... | 2010 | 20680579 |
the use of probiotics in healthy volunteers with evacuation disorders and hard stools: a double-blind, randomized, placebo-controlled study. | evacuation disorders and hard stools are common in industrialized countries, affecting on average 12% to 17% of the adult healthy population at any age. dietary supplementation with probiotic microorganisms may be useful in reducing the disorder. | 2010 | 20697291 |
exopolysaccharides produced by lactobacillus and bifidobacterium strains abrogate in vitro the cytotoxic effect of bacterial toxins on eukaryotic cells. | to evaluate the capability of the exopolysaccharides (eps) produced by lactobacilli and bifidobacteria from human and dairy origin to antagonize the cytotoxic effect of bacterial toxins. | 2010 | 20846331 |
evaluation of in vitro antagonism and of in vivo immune modulation and protection against pathogenic experimental challenge of two probiotic strains of bifidobacterium animalis var. lactis. | the aim of the present study was to compare the effect of intragastric administration with two strains of bifidobacterium animalis subsp. lactis (bifido a and bifido b), in gnotobiotic and conventional mice, challenged with salmonella typhimurium. in vitro antagonism test showed that the two strains were able to produce antagonistic substances against various pathogenic microorganisms. in an ex vivo antagonism test the production of antagonistic substances was observed only against three out ten ... | 2010 | 20848082 |
combined transcriptome and proteome analysis of bifidobacterium animalis subsp. lactis bb-12 grown on xylo-oligosaccharides and a model of their utilization. | recent studies have demonstrated that xylo-oligosaccharides (xos), which are classified as emerging prebiotics, selectively enhance the growth of bifidobacteria in general and of bifidobacterium animalis subsp. lactis strains in particular. to elucidate the metabolism of xos in the well-documented and widely used probiotic strain b. animalis subsp. lactis bb-12, a combined proteomic and transcriptomic approach was applied, involving dna microarrays, real-time quantitative pcr (qpcr), and two-dim ... | 2010 | 20851982 |
bifidobacterium animalis subsp. lactis bb-12 in reducing the risk of infections in infancy. | the impact of controlled administration of bifidobacterium animalis subsp. lactis bb-12 (bb-12) on the risk of acute infectious diseases was studied in healthy newborn infants. in this double-blind, placebo-controlled study, 109 newborn 1-month-old infants were assigned randomly to a probiotic group receiving a bb-12-containing tablet (n 55) or to a control group receiving a control tablet (n 54). test tablets were administered to the infants twice a day (daily dose of bb-12 10 billion colony-fo ... | 2010 | 20863419 |
bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes. | intestinal health requires the coexistence of eukaryotic self with the gut microbiota and dysregulated host-microbial interactions can result in intestinal inflammation. here, we show that colitis improved in t-bet(-/-)rag2(-/-) mice that consumed a fermented milk product containing bifidobacterium animalis subsp. lactis dn-173 010 strain. a decrease in cecal ph and alterations in short chain fatty acid profiles occurred with consumption, and there were concomitant increases in the abundance of ... | 2010 | 20921388 |
safety and tolerance of dietary supplementation with a canine-derived probiotic (bifidobacterium animalis strain ahc7) fed to growing dogs. | although probiotics are generally considered to be safe, their increasingly widespread use warrants better understanding of their risks in companion animals. this study evaluated the safety and tolerance of dietary supplementation with a canine-derived probiotic, bifidobacterium animalis strain ahc7 (prostora, procter & gamble pet care), fed to growing beagles beginning at approximately 6 months of age (11 males; 9 females). probiotic b. animalis ahc7 administered orally once per day at a dose o ... | 2010 | 20960415 |
intestinal bacteroides species associated with coeliac disease. | to characterise the predominant species of bacterial populations associated with duodenal biopsies of paediatric patients with active and treated coeliac disease. | 2010 | 20972239 |
persistence of probiotic strains in the gastrointestinal tract when administered as capsules, yoghurt, or cheese. | most clinical studies of probiotics use freeze-dried, powdered bacteria or bacteria packed in capsules. however, probiotics are commercially available in various food matrices, which may affect their persistence in the gastrointestinal tract. the objective of the study was to compare oral and faecal recovery during and after administration of a combination of lactobacillus rhamnosus gg and lc705, propionibacterium freudenreichii subsp. shermanii js, and bifidobacterium animalis subsp. lactis bb1 ... | 2010 | 21074284 |
safety and intestinal microbiota modulation by the exopolysaccharide-producing strains bifidobacterium animalis ipla r1 and bifidobacterium longum ipla e44 orally administered to wistar rats. | bifidobacterium animalis subsp. lactis ipla r1 and bifidobacterium longum ipla e44 strains were tested for their safety and ability to modulate the intestinal microbiota in vivo. chemically simulated gastrointestinal digestion showed considerably lower survival of e44 than r1 strain, the first microorganism also being more sensitive to refrigerated storage in 10% skimmed milk at 4°c. harmful glycosidic activities were absent, or at low levels, in the strains r1 and e44. both strains were sensiti ... | 2011 | 21078530 |
in vitro and in vivo antioxidant activity of bifidobacterium animalis 01 isolated from centenarians. | several studies reported the antioxidant activity of bifidobacteria using assays in vitro. in present study, the in vitro and in vivo antioxidant activity of bifidobacterium animalis 01 was investigated. culture supernatant, intact cells, and intracellular cell-free extracts of b. animalis 01 were involved in this study. the antioxidant assays in vitro included lipid peroxidation assay, 1,1-diphenyl-2-picrylhydrazyl (dpph) assay, hydroxyl radical ((•)oh) assay and superoxide anion (o₂⁻) assay. t ... | 2010 | 21132298 |
bifidobacterium animalis ahc7 protects against pathogen-induced nf-κb activation in vivo. | bifidobacteria and lactobacilli are among the early and important colonizers of the gastrointestinal tract and are generally considered to be part of a normal, healthy microbiota. it is believed that specific strains within the microbiota can influence host immune-reactivity and may play a role in protection from infection and aberrant inflammatory activity. one such strain, bifidobacterium animalis ahc7, has been previously shown to protect against salmonella typhimurium infection in mice and h ... | 2010 | 21176205 |
predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria lactobacillus acidophilus ncfm and bifidobacterium animalis subsp. lactis bi-07. | the effect of probiotic bacteria lactobacillus acidophilus ncfm and bifidobacterium lactis bi-07 on the composition of the lactobacillus group, bifidobacterium and the total bacterial population in feces from young children with atopic dermatitis was investigated. the study included 50 children randomized to intake of one of the probiotic strain or placebo. microbial composition was characterized by denaturing gradient gel electrophoresis, quantitative pcr and, in a subset of subjects, by pyrose ... | 2011 | 21204871 |
beneficial effects of a synbiotic supplement on self-perceived gastrointestinal well-being and immunoinflammatory status of healthy adults. | the use of synbiotics as health promoters is still poorly defined, and human intervention studies are scarce. this study was designed to evaluate the effects of a commercialized synbiotic product containing lactobacillus acidophilus la5, bifidobacterium animalis ssp. lactis bb-12, lactobacillus delbrueckii ssp. bulgaricus, lactobacillus paracasei ssp. paracasei, streptococcus thermophilus, and fructooligosaccharides on the self-reported gastrointestinal well-being and the immunoinflammatory stat ... | 2011 | 21244240 |
impact of bifidobacterium animalis subsp. lactis bb-12 and, lactobacillus acidophilus la-5-containing yoghurt, on fecal bacterial counts of healthy adults. | this randomized, placebo-controlled, double blind, parallel dose-response study investigated the impact of 4-week commercial yoghurt consumption supplemented with bifidobacterium animalis subsp. lactis (bb-12) and lactobacillus acidophilus (la-5) on fecal bacterial counts of healthy adults. fifty-eight volunteers were randomly assigned to three different groups: 1. placebo (no probiotic, no starter and no green tea extract); 2. yoptimal (10(9)cfu/100g of bb-12 and la-5 and 40mg of green tea extr ... | 2011 | 21296446 |
study to investigate the potential of probiotics in children attending school. | to determine if consumption of yogurt containing a high dose of probiotic (1×10(10) colony-forming unit per 100 ml), bifidobacterium animalis subsp. lactis (b. lactis), decreases absences in children 2-4 years attending daycare/school centers. | 2011 | 21326270 |
comparative analysis of an intestinal strain of bifidobacterium longum and a strain of bifidobacterium animalis subspecies lactis in cheddar cheese. | bifidobacteria cultures were incorporated into cheddar cheeses to conduct a comparative analysis between the commercially available strain bifidobacterium animalis ssp. lactis bb-12 and the wild-type intestinal isolate, bifidobacterium longum djo10a. they were incorporated as starter adjuncts in separate vats and as a mixed culture, and survival through manufacturing and cheese ripening was assessed. for cheese using only bb-12, the cells may have grown during cheese manufacture as 133% of the i ... | 2011 | 21338778 |
probiotic lactobacillus rhamnosus downregulates fcer1 and hrh4 expression in human mast cells. | to investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis. | 2011 | 21390145 |
adhesion of bile-adapted bifidobacterium strains to the ht29-mtx cell line is modified after sequential gastrointestinal challenge simulated in vitro using human gastric and duodenal juices. | according to the fao/who, in vitro criteria for selection of probiotics for food application consist of testing survival when confronted with gastrointestinal tract (git) challenge and the ability to colonize the colon. we used a model that simulated git transit using sequential immersion in gastric and duodenal juices of human origin to evaluate survival of bile-adapted bifidobacterium strains. bifidobacterium animalis tolerated gastric juice, whereas bifidobacterium longum showed poor survival ... | 2011 | 21419219 |
a randomised double-blind placebo-controlled trial with lactobacillus acidophilus la-5 and bifidobacterium animalis subsp. lactis bb-12 for maintenance of remission in ulcerative colitis. | to investigate the clinical effect of treatment with lactobacillus acidophilus la-5 and bifidobacterium animalis subsp. lactis bb-12 (probio-tec ab-25) to maintain remission in patients with ulcerative colitis. | 2011 | 21453880 |
establishment of an alternative phosphoketolase-dependent pathway for fructose catabolism in ralstonia eutropha h16. | the β-proteobacterium ralstonia eutropha h16 utilizes fructose and gluconate as carbon sources for heterotrophic growth exclusively via the entner-doudoroff pathway with its key enzyme 2-keto-3-deoxy-6-phosphogluconate (kdpg) aldolase. by deletion of the responsible gene eda, we constructed a kdpg aldolase-negative strain, which is disabled to supply pyruvate for energy metabolism from fructose or gluconate as sole carbon sources. to restore growth on fructose, an alternative pathway, similar to ... | 2011 | 21519932 |
the extracellular proteome of bifidobacterium animalis subsp. lactis bb-12 reveals proteins with putative roles in probiotic effects. | probiotics are live microorganisms that exert health-promoting effects on the human host, as demonstrated for numerous strains of the genus bifidobacterium. to unravel the proteins involved in the interactions between the host and the extensively used and well-studied probiotic strain bifidobacterium animalis subsp. lactis bb-12, proteins secreted by the bacterium, i.e. belonging to the extracellular proteome present in the culture medium, were identified by 2-de coupled with maldi-tof ms. among ... | 2011 | 21598393 |
effect of kynurenic acid on the viability of probiotics in vitro. | probiotics are bacteria that are commercially available as dietary supplements. one of the important properties of probiotics is their ability to survive in the intestine. recent evidence has identified kynurenic acid (kyna) as a bactericidal constituent of intestinal fluid. these data led us to study the influence of kyna on the viability of selected probiotics. we found that kyna supported the growth of bacteria in the probiotics acidolac (lactobacillus acidophilus, bifidobacterium) and lakcid ... | 2011 | 21602611 |
prebiotic effects of wheat arabinoxylan related to the increase in bifidobacteria, roseburia and bacteroides/prevotella in diet-induced obese mice. | alterations in the composition of gut microbiota--known as dysbiosis--has been proposed to contribute to the development of obesity, thereby supporting the potential interest of nutrients targeting the gut with beneficial effect for host adiposity. we test the ability of a specific concentrate of water-extractable high molecular weight arabinoxylans (ax) from wheat to modulate both the gut microbiota and lipid metabolism in high-fat (hf) diet-induced obese mice. | 2011 | 21695273 |
enzyme stability of microencapsulated bifidobacterium animalis ssp. lactis bb12 after freeze drying and during storage in low water activity at room temperature. | ôçé stability of enzymes such as +¦-galactosidase (+¦-gal), +¦-glucosidase (+¦-glu), lactate dehydrogenase (ldh), pyruvate kinase (pk), hexokinase (hk), and atpase of microencapsulated bifidobacterium animalis ssp. lactis bb12 after freeze-drying and after 10 wk of storage at low water activity (a(w) ) at room temperature was studied. bacteria were microencapsulated using alginate formulation with or without mannitol fortification (sodium alginate and mannitol [sam] and sodium alginate [sa], res ... | 2011 | 21696390 |
intestinal mucosal adherence and translocation of commensal bacteria at the early onset of type 2 diabetes: molecular mechanisms and probiotic treatment. | a fat-enriched diet modifies intestinal microbiota and initiates a low-grade inflammation, insulin resistance and type-2 diabetes. here, we demonstrate that before the onset of diabetes, after only one week of a high-fat diet (hfd), live commensal intestinal bacteria are present in large numbers in the adipose tissue and the blood where they can induce inflammation. this translocation is prevented in mice lacking the microbial pattern recognition receptors nod1 or cd14, but overtly increased in ... | 2011 | 21735552 |
incorporation of probiotic bacteria in whey cheese: decreasing the risk of microbial contamination. | for dairy products that are consumed fresh, contamination by spoilage microorganisms and pathogens from the environment is a major concern. contamination has been associated with a number of outbreaks of foodborne illnesses; however, consistent data pertaining to the microbial safety of whey cheeses specifically have not been reported. hence, the goals of this research effort were (i) to manufacture a probiotic whey cheese with bifidobacterium animalis and lactobacillus casei and (ii) to assess ... | 2011 | 21740724 |
in vitro evaluation of gastrointestinal survival of lactobacillus amylovorus dsm 16698 alone and combined with galactooligosaccharides, milk and/or bifidobacterium animalis subsp. lactis bb-12. | probiotic properties of lactobacillus amylovorus dsm 16698 were previously demonstrated in piglets. here, its potential as a human probiotic was studied in vitro, using the tim-1 system, which is fully validated to simulate the human upper gastrointestinal tract. to evaluate the effect of the food matrix composition on the survival of l. amylovorus dsm 16698 in tim-1, the microorganism was inoculated alone or with prebiotic galactooligosaccharides (gos), partially skimmed milk (psm) and/or comme ... | 2011 | 21741105 |
relevance of the bifidobacterium animalis subsp. lactis plasminogen binding activity in the human gastrointestinal microenvironment. | human plasmin(ogen) is regarded as a component of the molecular cross-talk between the probiotic species bifidobacterium animalis subsp. lactis and the human host. however, up to now, only in vitro studies have been reported. here we demonstrate that the probiotic strain b. animalis subsp. lactis bi07 is capable to recruit plasmin(ogen) present at physiological concentrations in crude extracts from human faeces. our results provide evidences that support the significance of the b. lactis-plasmin ... | 2011 | 21821753 |
obesity-associated gut microbiota is enriched in lactobacillus reuteri and depleted in bifidobacterium animalis and methanobrevibacter smithii. | background:obesity is associated with increased health risk and has been associated with alterations in bacterial gut microbiota, with mainly a reduction in bacteroidetes, but few data exist at the genus and species level. it has been reported that the lactobacillus and bifidobacterium genus representatives may have a critical role in weight regulation as an anti-obesity effect in experimental models and humans, or as a growth-promoter effect in agriculture depending on the strains.objectives an ... | 2011 | 21829158 |
analysis of the human intestinal epithelial cell transcriptional response to lactobacillus acidophilus, lactobacillus salivarius, bifidobacterium lactis and escherichia coli. | the complex microbial population residing in the human gastrointestinal tract consists of commensal, potential pathogenic and beneficial species, which are probably perceived differently by the host and consequently could be expected to trigger specific transcriptional responses. here, we provide a comparative analysis of the global in vitro transcriptional response of human intestinal epithelial cells to lactobacillus acidophilus ncfmôäó, lactobacillus salivarius ls-33, bifidobacterium animalis ... | 2010 | 21831765 |
effects of synbiotic fermentation products on primary chemoprevention in human colon cells. | the consumption of synbiotics, a mixture of probiotics and indigestible food constituents such as dietary fiber, has been reported to reduce colon cancer risk. we investigated the effects of fermented wheat aleurone enriched with the probiotics lactobacillus rhamnosus gg/bifidobacterium animalis supsp. lactis on the gene expression and functional end points related to cellular defence in ht29 and primary human colon cells. aleurone was digested and fermented in vitro with/without probiotics. the ... | 2011 | 21840698 |
late rather than early responses of human dendritic cells highlight selective induction of cytokines, chemokines and growth factors by probiotic bacteria. | the probiotic properties of commensal bacteria including lactobacilli and bifidobacteria are likely to be determined at least in part by their effects on dendritic cells. like traditional immune stimulants such as lipopolysaccharides (lps), probiotic bacteria promote maturation of cultured human dendritic cells (dc) by inducing elevated expression of mhc-ii and co-stimulatory molecules. different effects have been reported on cytokine induction, especially of major regulatory cytokines such as t ... | 2010 | 21840799 |
Longevity in mice is promoted by probiotic-induced suppression of colonic senescence dependent on upregulation of gut bacterial polyamine production. | Chronic low-grade inflammation is recognized as an important factor contributing to senescence and age-related diseases. In mammals, levels of polyamines (PAs) decrease during the ageing process; PAs are known to decrease systemic inflammation by inhibiting inflammatory cytokine synthesis in macrophages. Reductions in intestinal luminal PAs levels have been associated with intestinal barrier dysfunction. The probiotic strain Bifidobacterium animalis subsp. lactis LKM512 is known to increase inte ... | 2011 | 21858192 |
in vitro and in vivo antioxidant activity of exopolysaccharide fractions from bifidobacterium animalis rh. | the aim of the study was to purify the exopolysaccharides (eps) produced from bifidobacterium animalis rh, which was isolated from the feces of bama centenarians in guangxi of china, and evaluate their antioxidant activities in vitro and in vivo. 2 fractions, a neutral eps fraction (epsa) and an acidic eps fraction (epsb), were obtained and compared for antioxidative activity. in vitro, they both showed remarkable inhibition effect on lipid peroxidation and strong dpph radical scavenging activit ... | 2011 | 21875680 |
molecular characterization of bile salt hydrolase from bifidobacterium animalis subsp. lactis bi30. | the present work describes the identification, purification, and characterization of bile salt hydrolase (bsh) from bifidobacterium animalis subsp. lactis. the enzyme was purified to electrophoretic homogeneity by hydrophobic chromatography, ion-exchange chromatography and ultrafiltration. sds-page analysis of putative bsh and gel filtration revealed that the analyzed protein is presumably a tetramer composed of four monomers each of about 35 kda. the purified enzyme was analyzed by liquid chrom ... | 2011 | 21876374 |
Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12® and Lactobacillus paracasei ssp. paracasei, L. casei 431® in an influenza vaccination model: a randomised, double-blind, placebo-controlled study. | The present study investigated the ability of Bifidobacterium animalis ssp. lactis (BB-12®) and Lactobacillus paracasei ssp. paracasei (L. casei 431®) to modulate the immune system using a vaccination model in healthy subjects. A randomised, double-blind, placebo-controlled, parallel-group study was conducted in 211 subjects (56 % females, mean age 33·2 (sd 13·1) years). Subjects consumed a minimum of 109 colony-forming units of BB-12® (capsule) or L. casei 431® (dairy drink) or a matching place ... | 2011 | 21899798 |
genome sequence of the probiotic strain bifidobacterium animalis subsp. lactis cncm i-2494. | bifidobacterium animalis subsp. lactis cncm i-2494 is part of a commercialized fermented dairy product with documented health benefits revealed by multiple randomized placebo-controlled clinical trials. here we report the complete genome sequence of this strain, which has a circular genome of 1,943,113 bp with 1,660 open reading frames and 4 ribosomal operons. | 2011 | 21914878 |
secreted factors from bifidobacterium animalis subsp. lactis inhibit nf-κb-mediated interleukin-8 gene expression in caco-2 cells. | the objective of the present study was to evaluate the anti-inflammatory effects of bifidobacterium animalis subsp. lactis strain bb12 in stimulated caco-2 cells and to characterize the factors responsible for these anti-inflammatory effects. characterization and purification studies indicate that bb12's anti-inflammatory factors might include a 50-kda proteinaceous compound that is stable under a variety of heat and ph conditions. | 2011 | 21926200 |
Effects of daily consumption of probiotic yoghurt on inflammatory factors in pregnant women: a randomized controlled trial. | Previous studies have shown that inflammatory factors increases in pregnancy and is associated with several complications of pregnancy. The aim of this study was to assess effects of daily consumption of probiotic yoghurt on inflammatory factors in pregnant women. In a randomized clinical trial, seventy primigravid (the first pregnancy) and singleton pregnant women aged 18-30 years were assigned to two groups. Subjects consumed daily 200 g probiotic yoghurt containing Lactobacillus acidophilus L ... | 2011 | 21936251 |
the effect of probiotics on faecal microbiota and genotoxic activity of faecal water in patients with atopic dermatitis: a randomized, placebo-controlled study. | background: colonic microbiota is involved in the etiology of colon cancer according to several reports. studies also indicate that the microbiota differs between atopic patients and healthy subjects. objective: to evaluate whether a probiotic mix containing lactobacillus paracasei lpc-37, lactobacillus acidophilus 74-2, and bifidobacterium animalis subsp. lactis dgcc 420 can affect the microbiota and its genotoxic activity in healthy subjects and patients with atopic dermatitis (ad). methods: a ... | 2011 | 21963389 |
characterization of bifidobacteria suitable for probiotic use in calves. | in our previous experiment, the ten calves originated bifidobacterial strains were administered to calves and re-isolated. fingerprinting techniques used in this study enabled us to distinguish the surviving and non-surviving strains. only the species bifidobacterium animalis ssp. animalis and bifidobacterium longum ssp. suis were found to survive in the intestine. | 2011 | 21986032 |
genetic analysis and morphological identification of pilus-like structures in members of the genus bifidobacterium. | background: cell surface pili in gram positive bacteria have been reported to orchestrate the colonization of host tissues, evasion of immunity and the development of biofilms. so far, little if any information is available on the presence of pilus-like structures in human gut commensals like bifidobacteria. results and discussion: in this report, atomic force microscopy (afm) of various bifidobacterial strains belonging to bifidobacterium bifidum, bifidobacterium longum subsp. longum, bifidobac ... | 2011 | 21995649 |
structure of the high molecular weight exopolysaccharide produced by bifidobacterium animalis subsp. lactis ipla-r1 and sequence analysis of its putative eps cluster. | the bile adapted strain bifidobacterium animalis subsp. lactis ipla-r1 secretes a high molecular weight exopolysaccharide (hmw-eps) when grown on the surface of agar-mrsc. this eps is composed of l-rhamnopyranosyl, d-glucopyranosyl, d-galactopyranosyl and d-galactofuranosyl residues in the ratio of 3:1:1:1. linkage analysis and 1d and 2d nmr spectroscopy were used to show that the eps has a hexasaccharide repeating unit with the following structure: [see formula in text]. treatment of the eps wi ... | 2011 | 22000767 |
evaluation of chitoligosaccharides effect upon probiotic bacteria. | the main objective of the present study was to evaluate the antibacterial effect - through the determination of minimum inhibitory (and lethal) concentrations, as well as the possible prebiotic potential of chitooligosaccharides (cos) - through the determination of growth curves, on bifidobacterium animalis bb12, bifidobacterium animalis bo and lactobacillus acidophilus ki. atomic force microscopy was further used to obtain high resolution images of cos effects upon the cell morphology. our resu ... | 2012 | 22024313 |
Unraveling how probiotic yogurt works. | No matter what the advertisements are, or are not, allowed to say, it would be good to know if probiotic yogurt, in addition to its nutritional value, has a beneficial effect on the gut. In this issue, McNulty, Gordon and their colleagues describe a parallel series of human and animal studies designed to uncover the effects of probiotic yogurt on the gut microbiota. The intake of yogurt supplemented with five bacterial species, including the probiotic Bifidobacterium animalis, did not appreciabl ... | 2011 | 22030747 |