Publications

molecular determination and genotyping of cryptosporidium spp. in fecal and respiratory samples of industrial poultry in iran.	to identify the genotypes of prevalent cryptosporidia in broiler chickens in lorestan province, iran.	2014	25063279
cryptosporidiosis in broiler chickens in zhejiang province, china: molecular characterization of oocysts detected in fecal samples.	cryptosporidium is one of the most important parasites in poultry, and this pathogen can infect more than 30 avian species. the present study investigated the infection rate of cryptosporidium among broiler chicken flocks. a total of 385 fecal samples from broiler chickens in 7 regions of zhejiang province collected from november 2010 to january 2012 were examined by microscopy. thirty-eight (10%) samples were positive for cryptosporidium infection, and 3 genotypes (cryptosporidium baileyi, cryp ...	2014	25075975
effects of different inoculation routes on the parasitic sites of cryptosporidium baileyi infection in chickens.	cryptosporidiosis is prevalent in domesticated, caged, and wild birds. cryptosporidium baileyi, an ascendant species of avian cryptosporidium, is an important pathogen. it causes respiratory disease in chickens, especially chickens younger than 50 days. in this study, sem, histological, semi-quantitative pcr, and nested pcr techniques were used to explore the impact of different inoculation routes on sites of c. baileyi infection in chickens. results showed that inoculation with sporozoites or o ...	2014	25148714
diagnosis of gastric cryptosporidiosis in birds using a duplex real-time pcr assay.	three species and several genotypes of cryptosporidium can infect the epithelial surface of the bursa of fabricius, the respiratory tract, the proventriculus, the intestine, and the urinary tract in birds. there is reason to believe that gastric cryptosporidiosis in birds is caused by cryptosporidium galli and cryptosporidium avian genotype iii, resulting in a chronic illness of the proventriculus that can lead to a debilitating and fatal clinical condition in birds of the orders passeriformes a ...	2014	25155280
natural infection of cryptosporidium muris in ostriches (struthio camelus).	a total of 303 fecal samples were collected from ostriches (struthio camelus) and 31 samples (10.2%) were cryptosporidium-positive upon microscopic analysis. the infection rate was 27.6% in ostriches aged 16-60 days, 1.2% in those aged 61-180 days, and 20.4% in those aged >10 years. the cryptosporidium-positive isolates were genotyped with a restriction fragment length polymorphism analysis and dna sequence analysis of the small subunit (ssu) rrna gene. the 22 isolates from ostriches aged >10 ye ...	2014	25178556
an in vitro model of infection of chicken embryos by cryptosporidium baileyi.	cryptosporidiosis is one of the most prevalent parasitic infections in domesticated, caged and wild birds. cryptosporidium baileyi is the most common species reported in a wide range of avian hosts. although this parasite is well investigated, there is no adequate in vitro model for its endogenous development, and therefore, knowledge of each life cycle phase is scarce. in the present study, an in vitro model for c. baileyi in chicken embryos was developed and the complete life cycle investigate ...	2014	25448358
molecular characterization of cryptosporidium spp. in domestic pigeons (columba livia domestica) in guangdong province, southern china.	to investigate the prevalence and assess the zoonotic transmission burden of cryptosporidium species in domestic pigeons in guangdong province, southern china, 244 fecal samples were collected from four pigeon breeding farms between june 2012 and march 2013. cryptosporidium oocysts were purified by sheather's sugar flotation technique and characterized by dna sequencing of small subunit ribosomal rna (ssu rrna) gene. cryptosporidium species were determined by comparison of sequences with corresp ...	2015	25773186
first finding of spontaneous infections with cryptosporidium baileyi and c. meleagridis in the red-legged partridge alectoris rufa from an aviary in the czech republic.	this paper represents the first report of spontaneous infection with cryptosporidium baileyi and cryptosporidium meleagridis in the red-legged partridge (alectoris rufa), as well as the percentage of positive samples and age-associated dynamics of cryptosporidial infections in an aviary in the czech republic. the entire infection process was monitored over two semesters (july-december 2012 and 2013) until release of birds for hunting purposes. coprological examination of 663 pooled fecal samples ...	2015	25814162
ascaridia nymphii n. sp. (nematoda: ascaridida) from the alimentary tract of a severely emaciated dead cockatiel nymphicus hollandicus.	this report describes ascaridia nymphii n. sp., a new species isolated from the alimentary tract of cockatiel nymphicus hollandicus in japan. more than 63 nematodes were found in the formalin-fixed small intestine, ventriculus, proventriculus and crop of a 48-day-old young cockatiel that died after exhibiting severe emaciation. no nematode eggs were observed in the faecal examination performed while the cockatiel was alive, but cryptosporidium oocysts were found. the intestinal mucosa was damage ...	2015	26276643
dynamics of th17 associating cytokines in cryptosporidium parvum-infected mice.	cryptosporidium parvum commonly inhabits the intestinal tract of animals and humans and can cause acute watery diarrhea and weight loss. however, host immune responses to cryptosporidium infections are not fully understood. il-17 (also called il-17a) is a pro-inflammatory cytokine of th17 cells that plays a role in the host response to cryptosporidium baileyi infection. the present study examined levels of il-17-specific messenger rna (mrna) and th17 associating cytokines in c. parvum-infected i ...	2016	26593737
cryptosporidium infections of ring-necked pheasants (phasianus colchicus) from an intensive artificial breeding programme in the czech republic.	from july to november 2012, preliminary coprological examinations were carried out on 85 pooled faecal samples of different aged ring-necked pheasants (phasianus colchicus) (hatches from may until july) from an intensive artificial breeding programme in the czech republic. cryptosporidium oocysts were detected in 12 samples (14.1 %) of ages >12 weeks (august-september). these results were supported by findings of cryptosporidium baileyi and cryptosporidium meleagridis oocysts in intestinal, or c ...	2016	26815038
multiyear survey of coccidia, cryptosporidia, microsporidia, histomona, and hematozoa in wild quail in the rolling plains ecoregion of texas and oklahoma, usa.	we developed nested pcr protocols and performed a multiyear survey on the prevalence of several protozoan parasites in wild northern bobwhite (colinus virginianus) and scaled quail (callipepla squamata) in the rolling plains ecoregion of texas and oklahoma (i.e. fecal pellets, bird intestines and blood smears collected between 2010 and 2013). coccidia, cryptosporidia, and microsporidia were detected in 46.2%, 11.7%, and 44.0% of the samples (n = 687), whereas histomona and hematozoa were undetec ...	2017	27222431
efficacy of a potential dna vaccine encoding cryptosporidium baileyi rhomboid protein against homologous challenge in chickens.	the parasite cryptosporidium baileyi can infect the larynx, trachea, bursa and cloaca of poultry, causing high mortality during severe infection and leading to substantial economic losses of the poultry industry. the rhomboid protein is very important in cryptosporidium infection. in this study, a nucleic acid based vaccine candidate pegfp-cbrom was constructed. after orally challenging with c. baileyi oocysts, the corresponding immune responses induced were analyzed and the immunoprotective eff ...	2016	27369569
annotated draft genome sequences of three species of cryptosporidium: cryptosporidium meleagridis isolate ukmel1, c. baileyi isolate tamu-09q1 and c. hominis isolates tu502_2012 and ukh1.	human cryptosporidiosis is caused primarily by cryptosporidium hominis, c. parvum and c. meleagridis. to accelerate research on parasites in the genus cryptosporidium, we generated annotated, draft genome sequences of human c. hominis isolates tu502_2012 and ukh1, c. meleagridis ukmel1, also isolated from a human patient, and the avian parasite c. baileyi tamu-09q1. the annotation of the genome sequences relied in part on rnaseq data generated from the oocyst stage of both c. hominis and c. bail ...	2016	27519257
molecular identification of enterocytozoon bieneusi, cryptosporidium, and giardia in brazilian captive birds.	a total of 85 fecal samples from captive birds collected from october 2013 to september 2014 in uberlândia and belo horizonte in the state of minas gerais (brazil) were evaluated for the presence of enterocytozoon bieneusi, cryptosporidium, and giardia by pcr. of these, three birds were found positive for e. bieneusi (3.5%), two for cryptosporidium (2.3%), and one for giardia (1.2%). two genotypes of e. bieneusi were detected by nucleotide sequence analysis of the its region, genotypes d and per ...	2017	27815734
proventriculitis associated with cryptosporidium baileyi in a snowy owl (bubo scandiacus) and its epidemiological investigation.	we describe an unusual case of proventriculitis associated with cryptosporidium baileyi in a 7-wk-old snowy owl (bubo scandiacus) chick kept at a zoo. necropsy of this animal revealed diffuse mucosal thickening of the proventriculus. subsequent histopathological examinations of the proventriculus showed marked ductal epithelial hyperplasia with intestinal metaplasia and severe inflammatory cell infiltration in the lamina propria and submucosa. these lesions were associated with numerous periodic ...	2017	28528556
molecular diagnosis and characterization of cryptosporidium spp. in turkeys and chickens in germany reveals evidence for previously undetected parasite species.	a total of 256 fecal specimens were randomly collected from farmed poultry in germany and screened for the presence of cryptosporidium spp. by pcr and further characterized by direct automated dna sequencing. using a nested pcr amplifying approximately 830 bp 18s rdna fragment, 7.03% (n = 18) of the samples were cryptosporidium-positive. in detail, cryptosporidium was detected in 9.3% (8/86) of turkeys, 5.7% (9/158) of broilers and 8.3% (1/12) of layers. after dna sequencing, cryptosporidium par ...	2017	28575116