Publications

comparative analysis of the proteins with tandem repeats from 8 microsporidia and characterization of a novel endospore wall protein colocalizing with polar tube from nosema bombycis.	as a common feature of eukaryotic proteins, tandem amino acid repeat has been studied extensively in both animal and plant proteins. here, a comparative analysis focusing on the proteins having tandem repeats was conducted in 8 microsporidia, including four mammal-infecting microsporidia (encephalitozoon cuniculi, encephalitozoon intestinalis, encephalitozoon hellem and encephalitozoon bieneusi) and four insect-infecting microsporidia (nosema apis, nosema ceranae, vavraia culicis and nosema bomb ...	2017	28321967
molecular characterization and expression analysis of turtle protein in silkworm that is associated with nosema bombycis infection.	in this report, we describe the cloning and characterization of a member of the immunoglobulin superfamily (igsf); i.e., turtle. the cdna of turtle was cloned from the silkworm bombyx mori using the rapid amplification of cdna ends (race) technique. three isoforms of bombyx turtle were obtained, including bmtutl-464, bmtutl-519, and bmtutl-810. the three isoforms had identical 27-amino acid signal peptides and four extracellular immunoglobulin (ig) domains (igi-igiv). sequence similarity and phy ...	2017	28465230
identification of a new subtilisin-like protease nbslp2 interacting with cytoskeletal protein septin in microsporidia nosema bombycis.	nosema bombycis is the pathogen of pébrine which brings heavy losses to sericulture every year. as a member of serine proteases, subtilisin-like protease (slp) is related to the pathogenicity in fungi. in this study, we characterized a novel 63.8kda subtilisin-like protease nbslp2 with a predicted transmembrane domain from microsporidia, n. bombycis. rt-pcr showed that the transcript of nbslp2 was detected from third day post infection. immunofluorescence assay (ifa) indicated that nbslp2 mainly ...	2017	28625841
easy labeling of proliferative phase and sporogonic phase of microsporidia nosema bombycis in host cells.	microsporidia are eukaryotic, unicellular parasites that have been studied for more than 150 years. these organisms are extraordinary in their ability to invade a wide range of hosts including vertebrates and invertebrates, such as human and commercially important animals. a lack of appropriate labeling methods has limited the research of the cell cycle and protein locations in intracellular stages. in this report, an easy fluorescent labeling method has been developed to mark the proliferative ...	2017	28640848
comparative proteomic analysis of differentially expressed proteins in the bombyx mori fat body during the microsporidia nosema bombycis infection.	nosema bombycis is an obligate intracellular parasite, which can cause pébrine disease. to investigate the effects of n. bombycis infection, 5th-instar silkworms were challenged with n. bombycis isolate cq1, and two-dimensional gel electrophoresis analysis was performed to analyze the differentially expressed proteins in infected and uninfected silkworm fat bodies 1, 2, 4, 6 and 8days post-infection (dpi). 46 differentially expressed proteins were identified at the 5 time points using maldi-tof/ ...	2017	28668257
divergence of a tandem duplication of manganese superoxide dismutase in nosema bombycis.	manganese superoxide dismutase (mnsod) is a key enzyme in the protection of cells from oxidative stress. a tandem duplication of the mnsod gene (nbmnsod1 and nbmnsod2) in the genome of nosema bombycis, a parasite of the silkworm bombyx mori, was previously identified. here, we compare the protein structures of nbmnsod1 and nbmnsod2 and characterize these two proteins in terms of cellular localization, timing of transcription, protein structure, and enzyme activity. despite a similarity in the pr ...	2017	28691191
functional characterization of an aquaporin from a microsporidium, nosema bombycis.	microsporidia are a diverse group of eukaryotic organisms, capable of causing parasitic infections in both vertebrates and invertebrates. during the germination process, there is an increase in the osmotic pressure of microsporidian spores. as part of this study, we cloned a homologous aquaporin gene in nosema bombycis, and named it nosema bombycis aquaporin (nbaqp). sequence analysis revealed that the nbaqp contains an open reading frame with a length of 750 bp and encodes a polypeptide of 249 ...	2017	28749993