Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| identifying the determinants in the equatorial domain of buchnera groel implicated in binding potato leafroll virus. | luteoviruses avoid degradation in the hemolymph of their aphid vector by interacting with a groel homolog from the aphid's primary endosymbiotic bacterium (buchnera sp.). mutational analysis of groel from the primary endosymbiont of myzus persicae (mpb groel) revealed that the amino acids mediating binding of potato leafroll virus (plrv; luteoviridae) are located within residues 9 to 19 and 427 to 457 of the n-terminal and c-terminal regions, respectively, of the discontinuous equatorial domain. ... | 2000 | 10775590 |
| characterization of nicotinic acetylcholine receptors from the insects aphis craccivora, myzus persicae, and locusta migratoria by radioligand binding assays: relation to thiamethoxam action. | thiamethoxam, a new neonicotinoid insecticide acting at nicotinic acetylcholine receptors, was characterized in competition binding assays with [3-h]-imidacloprid, a specific nicotinic ligand, using membranes from the aphids aphis craccivora and myzus persicae, and from the locust locusta migratoria. in all insects, scatchard analysis suggested two binding sites for imidacloprid with kd values in the range of 1 nm and 10 nm, respectively. the hill values were significantly below 1 (range of 0.63 ... | 2000 | 10789501 |
| characterization and genomic analysis of tobacco vein clearing virus, a plant pararetrovirus that is transmitted vertically and related to sequences integrated in the host genome. | a previously undescribed caulimo-like virus was identified in the hybrid tobacco species nicotiana edwardsonii, and was named tobacco vein clearing virus (tvcv) after the symptoms associated with its occurrence in this plant. the virions of tvcv are 50 nm in diameter and are composed of a 45 kda capsid protein and a 7767 bp dsdna genome. each strand of the genome is interrupted by a site-specific discontinuity. in genome sequence and arrangement of orfs tvcv was most similar to cassava vein mosa ... | 2000 | 10811941 |
| salivary proteins of aphids, a pilot study on identification, separation and immunolocalisation. | salivary proteins (sps) of schizaphis graminum, acyrthosiphon pisum and myzus persicae were studied after probing and feeding on different artificial diets. salivary sheaths as well as apical lumps of saliva were found, presumably representing subsequently excreted saliva of different types. phenoloxidase, pectinase and peroxidase activities were detected by staining the enzyme-converted products, thus confirming these enzyme activities found earlier by others. proteinase and cellulase were not ... | 2000 | 10818245 |
| insecticidal and mutagenic evaluation of two annonaceous acetogenins. | annonaceous acetogenins represent a new class of bioactive compounds whose primary mode of action is the inhibition of nadh-ubiquinone oxidoreductase. given the potential pesticidal use of such a class of compounds, we have further evaluated the antifeedant and insecticidal effects of squamocin and annonacin, two annonaceous acetogenins, on spodoptera littoralis, leptinotarsa decemlineata, and myzus persicae. additionally, to partially assess their environmental risk, we have also tested their m ... | 2000 | 10869199 |
| methylation and expression of amplified esterase genes in the aphid myzus persicae (sulzer). | most populations of the aphid myzus persicae have amplified genes (up to 80 copies) encoding the insecticide-detoxifying esterase e4. this paper reports the analysis of methylation of the e4 gene and its flanking dna with the use of methylation-sensitive restriction enzymes, cpg profiling and bisulphite sequencing. in combination these show that e4 has 5-methylcytosine confined to cpg doublets, as previously shown for vertebrate genomes; this is the first such report for an insect gene. the meth ... | 2000 | 10903149 |
| insect nicotinic acetylcholine receptor: conserved neonicotinoid specificity of [(3)h]imidacloprid binding site. | the insect nicotinic acetylcholine receptor (nachr) is a major target for insecticide action. the rapidly expanding use of neonicotinoid insecticides of varied structures makes it increasingly important to define similarities and differences in their action, particularly for the first-generation chloropyridinyl compounds versus the second-generation chlorothiazolyl derivatives. we have shown with musca domestica that a convenient and relevant determination of the neonicotinoid insecticide target ... | 2000 | 10936213 |
| structure- and species-dependent insecticidal effects of neo-clerodane diterpenes. | several natural neo-clerodane diterpenoids isolated from linaria saxatilis and some semisynthetic derivatives were tested against several insect species with different feeding adaptations. the antifeedant tests showed that the oliphagous leptinotarsa decemlineata was the most sensitive insect, followed by the aphid myzus persicae. the polyphagous spodoptera littoralis was not deterred by these diterpenoids; however, following oral administration, some of these compounds did have postingestive an ... | 2000 | 10956169 |
| characterization of cucurbita maxima phloem serpin-1 (cmps-1). a developmentally regulated elastase inhibitor. | we report on the molecular, biochemical, and functional characterization of cucurbita maxima phloem serpin-1 (cmps-1), a novel 42-kda serine proteinase inhibitor that is developmentally regulated and has anti-elastase properties. cmps-1 was purified to near homogeneity from c. maxima (pumpkin) phloem exudate and, based on microsequence analysis, the cdna encoding cmps-1 was cloned. the association rate constant (k(a)) of phloem-purified and recombinant his(6)-tagged cmps-1 for elastase was 3.5 + ... | 2000 | 10960478 |
| host-correlated morphological variation of myzus persicae (hemiptera: aphididae) populations in greece. | morphological variation in nine characters of 157 clones of myzus persicae (sulzer) was examined by multivariate analysis. the clones were collected from peach, prunus persica, the primary host and the secondary hosts tobacco, nicotiana tabacum, cabbage, brassica oleracea, sugarbeet, beta vulgaris and pepper capsicum annuum. the 156 clones originated from various regions of greece, both in the north, where a large part of the population has an annual bisexual generation on peach, and in more sou ... | 2000 | 10996864 |
| cucumber mosaic virus mutants with altered physical properties and defective in aphid vector transmission. | two mutant strains of cucumber mosaic virus (cmv) were investigated with respect to virion stability and molecular determinants of aphid vector transmission. the mutant 2a1-mt-60x, derived from the mechanically passaged wild type 2a1-at, is poorly transmissible by the aphid aphis gossypii and not transmissible by the aphid myzus persicae, whereas the wild type virus is transmissible by both aphid species. the mutant phenotype was shown to be conferred by a single encoded amino acid change of ala ... | 2000 | 11040130 |
| lactones. 9. synthesis of terpenoid lactones-active insect antifeedants. | starting from (+)- and (-)-perillyl alcohols, via claisen rearrangement and iodolactonization, four enantiomeric pairs of gamma-lactones were obtained. the structures of compounds were established by both spectroscopic and crystallographic methods. the lactones were tested for antifeeding activity toward grain storage pests-the granary weevil beetle (sitophilus granarius l.), the khapra beetle (trogoderma granarium ev.), and the confused flour beetle (tribolium confusum duv.). the results of the ... | 2000 | 11052764 |
| the passage of potato leafroll virus through myzus persicae gut membrane regulates transmission efficiency. | potato leafroll virus (plrv) is transmitted by aphids in a persistent manner. although virus circulation within the aphid leading to transmission has been well characterized, the mechanisms involved in virus recognition at aphid membranes are still poorly understood. one isolate in our collection (plrv-14.2) has been shown to be non- or only poorly transmitted by some clones of aphids belonging to the myzus persicae complex. to determine where the transmission process was blocked within the aphi ... | 2001 | 11125153 |
| some epidemiological approaches to the control of aphid-borne virus diseases in seed potato crops in northern europe. | an account is given of progress during the last 30 years in knowledge of the epidemiology of diseases caused by aphid-borne viruses in seed potato production areas of northern europe. during this period, potato leafroll virus (plrv) and strains of potato virus y (pvy(o), pvy(n)) were consistently the most prevalent and harmful. the main factors influencing spread involve the amount of initial virus inoculum in seed crops, agricultural practices in relation to seed potato production, the status o ... | 2000 | 11137160 |
| extreme resistance to potato leafroll virus in potato cv. russet burbank mediated by the viral replicase gene. | high levels of field resistance to potato leafroll virus (plrv; genus: polerovirus; family: luteoviridae) were achieved by expression of the unmodified, full-length plrv replicase gene in potato plants cv. russet burbank. a high degree of resistance was also achieved, but less frequently, by expression of a truncated construct of the replicase gene. in limited testing, neither miss-frame nor antisense constructs of the replicase gene conferred resistance. the degree of resistance expressed among ... | 2000 | 11137161 |
| inheritance and meiotic behaviour of a de novo chromosome fusion in the aphid myzus persicae (sulzer). | a de novo tandem fusion between autosomes 2 and 3 (a2+3), arising in the course of laboratory crosses of sexual morphs of two clones of the aphid myzus persicae, was stable through more than 180 generations of parthenogenetic (clonal) reproduction. studies of its inheritance through the sexual phase, and segregation from an amplified esterase marker gene, showed that crossing over occurred during oogenesis, but not in spermatogenesis, confirming previous cytological observations. only a small nu ... | 2000 | 11151679 |
| differentiation of celosia mosaic virus and asparagus virus 1 based on biological properties. | an attempt was made to distinguish between celosia mosaic virus (cimv) and asparagus virus 1 (av-1) based on biological properties, which hitherto was obscured from serological data from previous work. the host range of av-1 was found to be a subset of that of cimv and av-1 was transmitted by the aphid myzus persicae which, on the other hand, did not transmit cimv. no evidence of cross-protection was obtained between these two viruses. | 2000 | 11155362 |
| molecular responses to aphid feeding in arabidopsis in relation to plant defense pathways. | little is known about molecular responses in plants to phloem feeding by insects. the induction of genes associated with wound and pathogen response pathways was investigated following green peach aphid (myzus persicae) feeding on arabidopsis. aphid feeding on rosette leaves induced transcription of two genes associated with salicylic acid (sa)-dependent responses to pathogens (pr-1 and bgl2) 10- and 23-fold, respectively. induction of pr-1 and bgl2 mrna was reduced in npr1 mutant plants, which ... | 2001 | 11161062 |
| direct and indirect sublethal effects of galanthus nivalis agglutinin (gna) on the development of a potato-aphid parasitoid, aphelinus abdominalis (hymenoptera: aphelinidae). | snowdrop lectin (galanthus nivalis agglutinin, gna), has been shown to confer partial resistance to two potato aphids myzus persicae and aulacorthum solani, when incorporated in artificial diet and/or expressed in transgenic potato. first-tier laboratory-scale experiments were conducted to assess the potential effect of gna on the aphid parasitoid aphelinus abdominalis. gna (0.1% w/v) was successfully delivered to macrosiphum euphorbiae via artificial diet and induced a reduced growth rate and i ... | 2001 | 11249943 |
| [3h]-methyllycaconitine: a high affinity radioligand that labels invertebrate nicotinic acetylcholine receptors. | nicotinic acetylcholine receptors (nachr) of insect and other invertebrates are heterogeneous and new tools are needed to dissect their multiplicity. [(3)h]-methyllycaconitine ([(3)h]-mla) is a novel radioligand which is a potent antagonist at vertebrate alpha7-type nachr. putative invertebrate nachr of the aphid myzus persicae, the moths heliothis virescens and manduca sexta, the fly lucilia sericata, and the squid loligo vulgaris were investigated in radioligand binding studies with [(3)h]-mla ... | 2001 | 11267892 |
| modified atmosphere treatments as a potential disinfestation technique for arthropod pests in greenhouses. | incidental transport of arthropods on plant material can be a significant mode of pest entry into greenhouses. we evaluated the use of controlled atmosphere treatments as a potential way to eliminate arthropod pests on plant propagules (i.e., cuttings or small rooted plants). lethal exposures to co2 or n2 were determined for common greenhouse pests including fungus gnat larvae, bradysia sp.; green peach aphid, myzus persicae (sulzer); sweetpotato whitefly, bemisia sp.; twospotted spider mite, te ... | 2001 | 11332835 |
| studies on the role of the minor capsid protein in transport of beet western yellows virus through myzus persicae. | beet western yellows virus (bwyv), family luteoviridae, is an icosahedral plant virus which is strictly transmitted by aphids in a persistent and circulative manner. virions cross two cellular barriers in the aphid by receptor-based mechanisms involving endocytosis and exocytosis. particles are first transported across intestinal cells into the haemolymph and then across accessory salivary gland cells for delivery to the plant via saliva. we identified the midgut part of the digestive tract as t ... | 2001 | 11458007 |
| population-scale laboratory studies of the effect of transgenic plants on nontarget insects. | studies of the effects of insect-resistant transgenic plants on beneficial insects have, to date, concentrated mainly on either small-scale "worst case scenario" laboratory experiments or on field trials. we present a laboratory method using large population cages that represent an intermediate experimental scale, allowing the study of ecological and behavioural interactions between transgenic plants, pests and their natural enemies under more controlled conditions than is possible in the field. ... | 2001 | 11472551 |
| microsatellite isolation, linkage group identification and determination of recombination frequency in the peach-potato aphid, myzus persicae (sulzer) (hemiptera: aphididae). | fifteen polymorphic microsatellite markers were used to establish linkage groups and relative rates of recombination in male and female myzus persicae (sulzer) (hemiptera: aphididae) (peach-potato aphid). we cloned nine markers from m. persicae and for these we report primer sequences and levels of allelic diversity and heterozygosity in four australian m. persicae populations. of the remaining six loci, four loci, also cloned from m. persicae, were obtained from g. malarky (natural history muse ... | 2001 | 11486508 |
| [studies on transgenic tobacco plants expressing two kinds of insect resistant genes]. | a synthetic bt cry1ac gene fussed with a secretary signal coding sequences at 5' end and a modified gna gene were used to construct a plant expression vector pbsgs1m+ and this vector was transferred into tobacco (nicotiana tabacum l.) by agrobacterium-mediated transformation method. results of pcr, southern blot and slot blot analysis indicated that both the chimeric bt cry1ac and gna genes were integrated into the genomes of transformed plants. western blot analysis indicated that at least the ... | 2001 | 11517599 |
| how species-specific is the phoretic relationship between the broad mite, polyphagotarsonemus latus (acari: tarsonemidae), and its insect hosts? | broad mite, polyphagotarsonemus latus (acari: tarsonemidae), is a serious plant pest in tropical and subtropical regions. phoretic associations between broad mite and two genera of whiteflies (insecta: homoptera: aleyrodidae). namely bemisia and trialeurodes, have been reported from different parts of the world. our purpose was to determine the specificity of the association between the mite and its phoretic hosts. two host plants, potatoes and cucumbers, were used to study these relationships i ... | 2001 | 11523917 |
| interactions of aphid herbivory and nitrogen availability on the total foliar glycoalkaloid content of potato plants. | in plant growth room (pgr) and open-air pot (oap) experiments, potato cvs king edward and maris piper were grown under two nitrogen levels or two different nitrogen release patterns. plants were subjected to infestation by peach potato aphids myzus persicae (homoptera: aphididae). total glycoalkaloid (ga) levels were measured in the foliage of both infested and non-infested plants, before, during and after aphid infestation. in the pgr experiment, aphid infestation reduced the amounts of total g ... | 2001 | 11545368 |
| [influence of temperature and relative humidity on epizootiological features of zoophthora anhuiensis in myzus persicae colonies]. | the colony of green peach aphid, myzus persicae(sulzer), which was initiated with 3 pathogen-free apterae and another 3 ones inoculated with the conidia of the entomophthoraceous fungus, zoophthora anhuiensis(li) humber, was maintained on detached cabbage leaves at the regimes of 10-25 degrees c and 90-100% of relative humidity(rh), and allowed for free reproduction and fungal infection. based on 26 day observations, it was showed that the development of z. anhuiensis in the colony was rapid at ... | 2001 | 11758389 |
| [predation on myzus persicae by propylaea japonica adults with different extents of starvation]. | the study showed that the functional response of predation on m. persicae by the female and male adults of p. japonica with different extents of starvation belonged to the type of holling ii. female adults had a larger attacking rate than male adults, but the predacious amount of m. persicae by female and male adult predators of different extent of starvation within 24 hrs had no significant difference. the predacious amount by female adult was larger than that of male. the significant differenc ... | 2000 | 11767536 |
| green peach aphid (homoptera: aphididae) action thresholds for controlling the spread of potato leafroll virus in idaho. | arbitrary green peach aphid, myzus persicae (sulzer), action thresholds (0, 5, 10, 20, and 40 aphids per 100 leaves) were tested in 3 yr of field experimentation to determine if they could be maintained and if they would significantly impact aphid densities and limit the incidence of potato leafroll virus (plrv). in 1997 and 1998, significant linear relationships between thresholds and final percentage of plrv (expressed as the percentage of tubers infected with plrv) were observed: there was a ... | 2001 | 11777033 |
| translocation and translaminar bioavailability of two neonicotinoid insecticides after foliar application to cabbage and cotton. | a laboratory study was undertaken to investigate the leaf systemic properties and the translaminar aphicidal activity of two commercialised neonicotinoid (chloronicotinyl) insecticides. for that purpose [14c]imidacloprid was subjected to uptake and translocation studies in cabbage and cotton after foliar application. foliar penetration and short-term translocation patterns of imidacloprid were similar in both plant species. nevertheless imidacloprid penetrated twice as much into cabbage leaves a ... | 2002 | 11838278 |
| polymerase chain reaction-restriction fragment length polymorphism of ribosomal internal transcribed spacer region analysis on polyacrylamide gel electrophoresis reveals two haplotypes coexisting in myzus persicae. | aphids are geographically and economically the most important group of phytophagous insects. they are responsible for various damage on cultivated plants. in our laboratory, we are interested in their ability to transmit phytoviruses such as rhabdovirus, cucumovirus, potyvirus, and caulimavirus which cause many crop diseases. the present work deals with the study of vectors as analyzed by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) of internal transcribed spacer ... | 2002 | 11840522 |
| volatiles from potato plants infected with potato leafroll virus attract and arrest the virus vector, myzus persicae (homoptera: aphididae). | the influence of viral disease symptoms on the behaviour of virus vectors has implications for disease epidemiology. here we show that previously reported preferential colonization of potatoes infected by potato leafroll virus (genus polerovirus) (luteovirus) (plrv) by alatae of myzus persicae, the principal aphid vector of plrv, is influenced by volatile emissions from plrv-infected plants. first, in our bioassays both differential immigration and emigration were involved in preferential coloni ... | 2002 | 11886636 |
| microsatellite variation in cyclically parthenogenetic populations of myzus persicae in south-eastern australia. | we examined the population structure of the introduced aphid, myzus persicae collected mainly from its primary host, prunus persica, in south-east australia. myzus persicae has been present in australia since at least 1893. samples were collected in the spring of 1998 from two mainland and three tasmanian localities and isofemale lines were established in the laboratory. the reproductive mode (life cycle), karyotype and 17-locus microsatellite genotype of each clone were determined. all populati ... | 2002 | 11920132 |
| fluorometric microplate assay to measure glutathione s-transferase activity in insects and mites using monochlorobimane. | elevated levels of glutathione s-transferases (gsts) play a major role as a mechanism of resistance to insecticides and acaricides in resistant pest insects and mites, respectively. such compounds are either detoxicated directly via phase i metabolism or detoxicated by phase ii metabolism of metabolites as formed by microsomal monooxygenases. here we used monochlorobimane (mcb) as an artificial substrate and glutathione to determine total gst activity in equivalents of single pest insects and sp ... | 2002 | 11950219 |
| transgenic potatoes with enhanced levels of nematode resistance do not have altered susceptibility to nontarget aphids. | cysteine proteinase inhibitors (cystatins) confer resistance to plant-parasitic nematodes when expressed in transgenic plants. the survival and growth of nymphs of the peach-potato aphid, myzus persicae, were adversely affected when cystatins were added to artificial diets. when aphids were clip-caged onto transgenic plants expressing chicken egg white cystatin (cewc) there was no adverse effect on aphid fitness. field populations of aphids on transgenic desiree potatoes, expressing cewc or a mo ... | 2002 | 11972767 |
| toxicity of biorational insecticides: activity against the green peach aphid, myzus persicae (sulzer). | the relationship between dose for each of four biorational insecticides (pyrethrins, neem extract, capsiacin extract, insecticidal soap) and mortality of the green peach aphid (myzus persicae) was determined using a laboratory bioassay. these insecticides were toxic to aphids and paired mixtures of the insecticides provided synergistic activity as measured by aphid mortality under the laboratory bioassay conditions. capsiacin extracts were found to provide low levels of mortality alone but acted ... | 2002 | 11975171 |
| effect of cabbage cultivars with varying levels of resistance to aphids on the performance of the parasitoid, aphidius colemani (hymenoptera: braconidae). | the effect of aphid resistance in plant cultivars on parasitism of myzus persicae (sulzer) and brevicoryne brassicae (linnaeus) by aphidius colemani viereck was investigated under laboratory conditions using three cultivars of common cabbage, brassica oleracea var. capitata. significantly greater aphid populations were found on cv. derby day (green-leaved, susceptible to both aphid species), regardless of the presence or absence of parasitoids, compared with cv. minicole (green-leaved, partially ... | 2002 | 12020362 |
| tritrophic interactions between cabbage cultivars with different resistance and fertilizer levels, cruciferous aphids and parasitoids under field conditions. | tritrophic interactions involving cabbage brassica oleracea var. capitata cultivars +/- fertilizer, brevicoryne brassicae (linnaeus) and myzus persicae (sulzer), and the parasitoids diaeretiella rapae (m'cintosh) and aphidius sp. were conducted in 1998 and 1999. brevicorne brassicae was the dominant aphid species on all cultivars +/- fertilizer, except for some treatments in late season 1998. ruby ball (red-leaved with antixenosis factors for b. brassicae alates) +/- fertilizer was consistently ... | 2002 | 12020363 |
| segregation of autosomes during spermatogenesis in the peach-potato aphid (myzus persicae) (sulzer) (hemiptera: aphididae). | most aphids are cyclic parthenogens, so are ideal models in studies of the mechanisms and consequences of sex and recombination. however, owing to a shortage of physical and genetic markers, there have been few studies of the most fundamental genetic processes in these organisms. for example, it is not known whether autosomal segregation during male spermatogenesis is in mendelian proportions: we address that question here. the aphid myzus persicae has a typical karyotype of 2n = 12 in females ( ... | 2002 | 12073550 |
| variation in the life cycle and morphology of the tobacco host-race of myzus persicae (hemiptera: aphididae) in relation to its geographical distribution. | morphological variation and life cycle category were examined in 121 clones of myzus persicae (sulzer). the clones were collected from tobacco from three localities in greece (xanthi, nea efessos and naphplion), one in germany (rheinstetten), one in france (bergerac) and one in spain (madrid). before morphometrics, all aphids were laboratory-reared on potato. the morphological variation was investigated using both canonical variates analysis and a novel non-parametric classification tree method. ... | 2002 | 12191438 |
| life cycle variation of myzus persicae (hemiptera: aphididae) in greece. | during the years 1995-1999 the life cycle category of 2797 clones of myzus persicae (sulzer) was examined. the clones originated from primary and secondary hosts from different localities of north and central greece and the island of crete in the south. four different overwintering life cycle strategies were found that have also been described for m. persicae and other heteroecious species previously. a geographical variation was found in the proportion of holocyclic clones from tobacco and othe ... | 2002 | 12191439 |
| production of sexual morphs by apterous virginoparae of myzus persicae (hemiptera: aphididae) in relation to pre- and postnatal exposure to short day conditions. | the production of males and females by apterae of a holocyclic clone of myzus persicae (sulzer) was studied in the laboratory by transferring apterous virginoparae from long day to short day conditions at different stages of their pre- and post-natal development. prenatal exposure was also examined in nine additional clones coming from different regions of greece. by increasing the number of short day cycles at 17 degrees c, the aphids switched to male production earlier and produced more males. ... | 2002 | 12191440 |
| bioassay and field-simulator studies of the efficacy of pymetrozine against peach-potato aphids, myzus persicae (hemiptera: aphididae), possessing different mechanisms of insecticide resistance. | twenty-one clones of the peach-potato aphid, myzus persicae (sulzer), carrying various combinations of known mechanisms of insecticide resistance were assessed for their response to the new pyridine azomethine compound, pymetrozine, in leaf-dip bioassays. pymetrozine was also applied as a foliar spray to populations of four different uk m persicae clones on potato plants in field-simulator chambers. neither study showed any evidence of cross-resistance to this compound. pymetrozine, used in conj ... | 2002 | 12192905 |
| field-simulator studies of insecticide resistance to dimethylcarbamates and pyrethroids conferred by metabolic- and target site-based mechanisms in peach-potato aphids, myzus persicae (hemiptera: aphididae). | a range of insecticides was applied at recommended application rates against populations of myzus persicae (sulzer) carrying various combinations of three insecticide resistance mechanisms (carboxylesterase-based metabolic resistance and two target-site mechanisms, known as mace and kdr), supported on either chinese cabbage or potatoes in field simulator cages. patterns of response were similar on both host species. mace conferred extreme resistance to pirimicarb and triazamate (dimethylcarbamat ... | 2002 | 12192906 |
| amino acid composition and nutritional quality of potato leaf phloem sap for aphids. | to define plant 'nutritional quality' for aphids, the causal basis of the variation in aphid performance between host plants of different developmental ages was explored using the aphids myzus persicae and macrosiphum euphorbiae on potato plants (solanum tuberosum). both aphid species performed better on developmentally young ('pre-tuber-filling') plants than on mature ('tuber-filling') plants. aphid performance did not vary with leaf phloem sucrose:amino acid ratio but could be related to chang ... | 2002 | 12200404 |
| [time-specific infection rate of beauveria bassiana on myzus persicae after topical inoculation of conidial suspension]. | conidial suspension(5 x 10(6) conidia.ml-1) of the entomopathogenic fungus, beauveria bassiana bbsg8702 was used to inoculate newly emerged apterous adults of the green peach aphid (myzus persicae) on detached cabbage leaves in order to measure time-specific infection rate of the fungal agent. the aphids of conidia attached were disinfected using 0.2% chlorothalonil solution at intervals of 8-56 h after inoculation and then maintained at 10 and 20 degrees c for daily observation. there was a sig ... | 2002 | 12216397 |
| lack of detectable genetic recombination on the x chromosome during the parthenogenetic production of female and male aphids. | we used polymorphic microsatellite markers to look for recombination during parthenogenetic oogenesis between the x chromosomes of aphids of the tribe macrosiphini. we examined the x chromosome because it comprises approximately 25 % of the genome and previous cytological observations of chromosome pairing and nucleolar organizer (nor) heteromorphism suggest recombination, although the same is not true for autosomes. a total of 564 parthenogenetic females of myzus clones with three distinct repr ... | 2002 | 12220127 |
| amplified esterase genes and their relationship with other insecticide resistance mechanisms in english field populations of the aphid, myzus persicae (sulzer). | myzus persicae samples were collected from populations present on a range of field crops between 1997 and 2000. a combination of biochemical, dna-based diagnostics and bioassays was used to assess the presence of three insecticide resistance mechanisms: elevated carboxylesterase (e4 or fe4), insensitive acetylcholinesterase and insensitive sodium channels (knockdown resistance, kdr). for the carboxylesterases, both the levels of enzyme and the type of gene present (e4 or fe4) were determined. th ... | 2002 | 12233178 |
| temporal and spatial dynamics of insecticide resistance in myzus persicae (hemiptera: aphididae). | advances in understanding insecticide resistance in the peach-potato aphid, myzus persicae (sulzer), at the genotypic, biochemical and molecular levels have led to rapid and precise methods for the detection of several resistance mechanisms (elevated carboxylesterase, modified acetylcholinesterase or mace, and knockdown resistance or kdr) in individual insects, and for monitoring their frequencies over space and time. this paper summarises the results of two long-term surveys of resistance dynam ... | 2002 | 12233179 |
| analysis of insecticide-resistant myzus persicae (sulzer) populations collected in italian peach orchards. | several populations of the green peach aphid, myzus persicae (sulzer), were collected in the most important italian peach-growing areas. in these populations, collected as primary colonies or as secondary ones, total esterase content and acetylcholinesterase sensitivity towards pirimicarb was assessed using biochemical assays. most of the individuals analysed generally exhibited an elevated esterase content, and in some cases acetylcholinesterase insensitivity. the implications of resistance lev ... | 2002 | 12233192 |
| incidence of infected myzus persicae alatae trapped in flight imply place-to-place dissemination of entomophthoralean fungi in aphid populations through migration. | 2002 | 12417214 | |
| antifeedant effects of marine halogenated monoterpenes. | in this work the antifeedant effects of the halogenated monoterpenes 1-13 were tested against several divergent insect species. these compounds have been isolated from plocamium cartilagineum (6 was isolated as an acetyl derivative), except for 4, which was isolated from pantoneura plocamioides. we have also included the semisynthetic derivatives 1a, 2a, and 7. compounds 1, 1a, 2, 4, 5, 7, 8-10, and 13 were antifeedants against leptinotarsa decemlineata with varying potencies. the aphids myzus p ... | 2002 | 12428955 |
| constitutive activation of jasmonate signaling in an arabidopsis mutant correlates with enhanced resistance to erysiphe cichoracearum, pseudomonas syringae, and myzus persicae. | in arabidopsis spp., the jasmonate (ja) response pathway generally is required for defenses against necrotrophic pathogens and chewing insects, while the salicylic acid (sa) response pathway is generally required for specific, resistance (r) gene-mediated defenses against both biotrophic and necrotrophic pathogens. for example, sa-dependent defenses are required for resistance to the biotrophic fungal pathogen erysiphe cichoracearum ucsc1 and the bacterial pathogen pseudomonas syringae pv. macul ... | 2002 | 12437300 |
| cauliflower mosaic virus is preferentially acquired from the phloem by its aphid vectors. | cauliflower mosaic virus (camv) is transmitted in a non-circulative manner by aphids following the helper strategy. helper proteins p2 and p3 act as a bridge between virions and the aphid cuticle. electronic monitoring of aphid stylet activities (epg technique), transmission tests and electron microscopy showed that camv is preferentially acquired from the phloem by its most common aphid vectors, brevycorine brassicae and myzus persicae. we also found that camv is semipersistently transmitted an ... | 2002 | 12466494 |
| two different genes encoding acetylcholinesterase existing in cotton aphid (aphis gossypii). | two acetylcholinesterase (ache) genes, ace1 and ace2, have been cloned from cotton aphid, aphis gossypii glover, using the rapid amplification of cdna ends (race) technique. to the best of our knowledge, this should be the first direct molecular evidence that multiple ache genes exist in insects. the ace1 gene was successfully amplified along its full length of 2371 bp. the open reading frame is 2031 bp long and encodes 676 amino acids (genbank accession no. af502082). the ace2 gene was amplifie ... | 2002 | 12502259 |
| synthesis and insecticidal activity of n-oxydihydropyrroles: 4-hydroxy-3-mesityl-5,5-dimethyl derivatives with various substituents at the 1-position. | a new series of n-oxydihydropyrrole derivatives was synthesized and evaluated for insecticidal activity against nilaparvata lugens and myzus persicae. various substituents were introduced to the 1-position of the dihydropyrrole ring, and the derivatives obtained exhibited systemic and/or contact insecticidal activity. the structure-activity relationship revealed that small alkyoxy and alkoxyalkoxy groups were more favorable than alkylcarbonyloxy, alkoxycarbonyloxy, or sulfonyloxy groups as subst ... | 2002 | 12506980 |
| a new virus infecting myzus persicae has a genome organization similar to the species of the genus densovirus. | the genomic sequence of a new icosahedral dna virus infecting myzus persicae has been determined. analysis of 5499 nt of the viral genome revealed five open reading frames (orfs) evenly distributed in the 5' half of both dna strands. three orfs (orf1-3) share the same strand, while two other orfs (orf4 and orf5) are detected in the complementary sequence. the overall genomic organization is similar to that of species from the genus densovirus: orfs 1-3 most likely encode the non-structural prote ... | 2003 | 12533713 |
| synthesis and insecticidal activity of novel n-oxydihydropyrroles: 4-hydroxy-3-mesityl-1-methoxymethoxy derivatives with various substituents at the 5-position. | this paper reports the synthesis and insecticidal activity of a series of novel 4-hydroxy-3-mesityl-1-methoxymethoxy-1,5-dihydro-2h-pyrrol-2-one derivatives, in which the substituents at the 5-position were varied with a number of alkyl and spirocycloalkyl groups. investigation of the structure-activity relationships revealed that small alkyl and spirocyclohexyl groups had a favorable effect on the insecticidal activity of these agents against myzus persicae. | 2003 | 12538006 |
| [biomass, sporulation and aphid-infecting virulence of pandora neoaphidis mycelia produced in repeated liquid culture]. | repeated culture of entomophthoraceous isolates is a suspectable factor leading to their virulence decline of biological variation. in the present study, an isolate of pandora neoaphidis, f98028, was repeatedly cultured six times at the regime of 20 degrees c and 80 r/min in sabouraud dextrose broth(sdb) at three inoculation ratio of 1/20, 2/20, and 4/20 (seed culture over fresh sdb). mycelial biomass(mb), sporulation capacity(sc), and virulence to the green peach aphid, myzus persicae, were ass ... | 2001 | 12549095 |
| selection by phage display of a variant mustard trypsin inhibitor toxic against aphids. | the mustard trypsin inhibitor, mti-2, is a potent inhibitor of trypsin with no activity towards chymotrypsin. mti-2 is toxic for lepidopteran insects, but has low activity against aphids. in an attempt to improve the activity of the inhibitor towards aphids, a library of inhibitor variants was constructed and cloned into the prlac3 phagemid vector. the library of 9.3 x 107 independent colonies was created by randomisation of a stretch of five consecutive codons in the reactive site. repeated sel ... | 2003 | 12581313 |
| laboratory evaluation of temperature effects on the germination and growth of entomopathogenic fungi and on their pathogenicity to two aphid species. | as part of an approach to select potential mycoinsecticides for aphid biocontrol, we investigated the effects of temperature on the growth, germination and pathogenicity of some hyphomycete fungi. commercially available mycoinsecticides (based on beauveria bassiana (balsamo) vuillemin and verticillium lecanii (zimmermann) viegas) and other isolates of b bassiana, v lecanii, metarhizium anisopliae (metschnikoff) sorokin and paecilomyces fumosoroseus (wize) brown & smith were evaluated. the rate o ... | 2003 | 12587869 |
| variation in response to neonicotinoid insecticides in peach-potato aphids, myzus persicae (hemiptera: aphididae). | laboratory bioassays applying the neonicotinoid insecticides imidacloprid, acetamiprid and nitenpyram against clones of the peach-potato aphid myzus persicae (sulzer) demonstrated that these compounds effectively circumvent the known carboxylesterase, modified acetylcholinesterase (mace) and knock-down (kdr) insecticide resistance mechanisms in this species. however, some clones showed cross-tolerance (up to 18-fold) of these compounds relative to susceptible standards. a survey assessing the fr ... | 2003 | 12587870 |
| biological characteristics of the mirids macrolophus costalis and macrolophus pygmaeus preying on the tobacco form of myzus persicae (hemiptera: aphididae). | biological and population parameters of the predatory bugs macrolophus costalis fieber and macrolophus pygmaeus rambur preying on the tobacco aphid myzus persicae (sulzer) were examined. tobacco was used as host plant and all experiments were carried out at 23 degrees c and l16:d8. in m. costalis, the developmental time for eggs and the total duration of nymphal instars was 13.1 and 22.2 days and in m. pygmaeus 13.0 and 20.2 days, respectively. both species completed their nymphal development fe ... | 2003 | 12593681 |
| european monitoring of resistance to insecticides in myzus persicae and aphis gossypii (hemiptera: aphididae) with special reference to imidacloprid. | the susceptibility to several insecticides of 16 and 8 strains of myzus persicae sulzer and aphis gossypii glover, respectively, received from different european countries in 2001 was investigated. most of the strains were derived from places known for their aphid resistance problems to conventional insecticides before imidacloprid was introduced. in many regions and agronomic cropping systems imidacloprid has been an essential part of aphid control strategies for a decade, and therefore the sus ... | 2003 | 12593682 |
| [role of contagious infection of pandora delphacis in suppression of myzus persicae colonies]. | the colonies of green peach aphid, myzus persicae, on detached cabbage leaves (8 replicates) were initiated with each including 3 apterae exposed to 'spore shower' of the entomophthoraceous fungus, pandora delphacis, to evaluate its potential for aphid control. the colonies were then allowed to freely propagate and infect from one to another for mycosis development at different regimes of temperature (10-30 degrees c) and relative humidity (74-100% rh). during a period of 30-day observation, aph ... | 2002 | 12625001 |
| quantitative assessment of the infection rate of the entomophthoraceous fungus, zoophthora anhuiensis against the green peach aphid myzus persicae. | a two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus, zoophthora anhuiensis (li) humber, on the green peach aphid, myzus persicae (sulzer). firstly, a standard time-dose-mortality relationship, established by modeling data from bioassay 1 at varying conidial dosages (0.4 - 10.4 conidia/mm2) of z. anhuiensis f97028, was used to yield an estimate of expected mortality probability at a given dosage. secondly, bioassay 2 was conducted by simult ... | 2006 | 12656350 |
| genetic, morphological, and virulence characterization of the entomopathogenic fungus verticillium lecanii. | in order to clarify relationships among genetic diversity, virulence, and other characteristics of conidia, 46 isolates of verticillium lecanii from various hosts and geographical locations were examined. the internal transcribed spacer (its) and intergenic spacer (igs) regions of ribosomal dna (rdna), mitochondrial small subunit rdna (mt-srdna) and beta-tubulin were analyzed by pcr-rflp. pcr-single stranded conformational polymorphism (sscp) was performed on regions of the mitochondrial large s ... | 2003 | 12676554 |
| co-existence of different host-adapted forms of the myzus persicae group (hemiptera: aphididae) in southern italy. | multivariate morphometric analysis (method of canonical variates) was used to compare 38 parthenogenetic lineages and three field collections of aphids of the myzus persicae (sulzer) group sampled on peach and tobacco in the caserta region of southern italy. comparisons were also made with the morphology of lineages collected on peach in lehonia, in central eastern greece away from tobacco-growing regions, and on tobacco in naphplion, in southern greece. the lineages were measured after partheno ... | 2003 | 12699534 |
| tritrophic interactions between transgenic potato expressing snowdrop lectin (gna), an aphid pest (peach-potato aphid; myzus persicae (sulz.) and a beneficial predator (2-spot ladybird; adalia bipunctata l.). | tritrophic interactions between transgenic potato expressing the insecticidal lectin from snowdrop (galanthus nivalis agglutinin; gna), an aphid pest, myzus persicae (sulz.), and a beneficial predator, the 2-spot ladybird (adalia bipunctata l.) were investigated. clonal plants expressing gna at 0.1-0.2% total soluble protein in leaves were used. no significant effects on development and survival of ladybird larvae fed on aphids from these transgenic plants were observed, with larval survival in ... | 2003 | 12739890 |
| performance of myzus persicae (hemiptera: aphididae) clones on different host-plants and their host preference. | the performance of eighteen clones of myzus persicae (sulzer) on pepper and tobacco plants at 20 degrees c and l16:d8 and the choice of young adult apterae between tobacco and pepper leaf-discs were examined. the clones were collected from weeds and peach in two tobacco-growing regions: katerini, northern greece and karditsa, central greece (only from weeds) and from lehonia, central eastern greece where tobacco is not cultivated. all clones did well on both hosts. however, the analysis of data ... | 2003 | 12762865 |
| effects of artificial diet containing gna and gna-expressing potatoes on the development of the aphid parasitoid aphidius ervi haliday (hymenoptera: aphidiidae). | aphid parasitoids are important biological control agents. the possibility arises that whilst foraging on insect-resistant transgenic plants, they are themselves at risk from direct and indirect effects of the expression of a transgene used to control the pest species. a liquid artificial diet was successfully used to deliver the snowdrop lectin (galanthus nivalis agglutinin; gna) to the peach-potato aphid, myzus persicae. bioassays utilising artificial diet incorporating gna, and excised leaves ... | 2001 | 12770142 |
| snowdrop lectin (gna) has no acute toxic effects on a beneficial insect predator, the 2-spot ladybird (adalia bipunctata l.). | two-spot ladybird (adalia bipunctata l.) larvae were fed on aphids (myzus persicae (sulz.)) which had been loaded with snowdrop lectin (galanthus nivalis agglutinin; gna) by feeding on artificial diet containing the protein. treatment with gna significantly decreased the growth of aphids. no acute toxicity of gna-containing aphids towards the ladybird larvae was observed, although there were small effects on development. when fed a fixed number of aphids, larvae exposed to gna spent longer in th ... | 2000 | 12770202 |
| comparison of newly isolated cuticular protein genes from six aphid species. | this paper reports on the first aphids' cuticular proteins. one gene (mpcp1) was obtained by screening a cdna library of myzus persicae with antibodies to a lepidopteran cuticle protein. mpcp1 presents a putative signal peptide, a central extended r&r domain, flanked by n- and c-terminal repeats of alanine, tyrosine and proline. the mrna of mpcp1 could be detected in a larval and in adult stages. primers based on mpcp1 allowed isolating and comparing cuticle protein genes from five aphid species ... | 2003 | 12826098 |
| [effect of plant alcohol extracts on vegetable aphids and their parasitoids]. | the repelling and controlling effect of alcohol extracts from 8 common plant species on 3 major vegetable aphids (myzus persicae, aphis gossypii and lipaphis erysimi) were studied in the laboratory, and the effect on their parasitoid was also studied. the results showed that the test 8 plant species had some effects on 3 aphids. the plant alcohol extracts from tephrosia vogelli and cinnamomum camphora had the most effect. the deterrent rate on myzus persicae, aphis gossypii and lipaphis erysimi ... | 2003 | 12827881 |
| synthesis and insecticidal activity of novel n-oxydihydropyrrole derivatives with a substituted spirocyclohexyl group. | a series of 5-spirocyclohexyl-3-(2,6-dimethylphenyl)-1,5-dihydro-2h-pyrrol-2-one derivatives (3) with various substituents on the spirocyclohexyl ring was synthesized and evaluated for its insecticidal activity against the aphid, myzus persicae. substituents at the 1- and 4-positions of the dihydropyrrole ring were also varied to optimize the activity. an investigation of the structure-activity relationship revealed that methoxy, alkoxyalkoxy, ethylenedioxy and methoxyimino groups were favorable ... | 2003 | 12843647 |
| effects of earthworms and organic litter distribution on plant performance and aphid reproduction. | human management practices and large detritivores such as earthworms incorporate plant litter into the soil, thereby forming a heterogeneous soil environment from which plant roots extract nutrients. in a greenhouse experiment we investigated effects of earthworms and spatial distribution of (15)n-labelled grass litter on plants of different functional groups [ lolium perenne (grass), plantago lanceolata (forb), trifolium repens (legume)]. earthworms enhanced shoot and root growth in l. perenne ... | 2003 | 12844255 |
| an amino acid substitution on the second acetylcholinesterase in the pirimicarb-resistant strains of the peach potato aphid, myzus persicae. | cdnas encoding two acetylcholinesterases (aches) were isolated from the peach potato aphid, myzus persicae. mpache1 was orthologous and mpache2 was paralogous with the ace of drosophila melanogaster. the deduced amino acid sequence of mpache1 cdna was identical between the pirimicarb susceptible and resistant strains. however, a single amino acid substitution of ser431phe on mpache2 was found in the pirimicarb resistant strains. this substitution was located in the acyl pocket of the enzyme and ... | 2003 | 12849975 |
| analogous pleiotropic effects of insecticide resistance genotypes in peach-potato aphids and houseflies. | we show that single-point mutations conferring target-site resistance (kdr) to pyrethroids and ddt in aphids and houseflies, and gene amplification conferring metabolic resistance (carboxylesterase) to organophosphates and carbamates in aphids, can have deleterious pleiotropic effects on fitness. behavioural studies on peach-potato aphids showed that a reduced response to alarm pheromone was associated with both gene amplification and the kdr target-site mutation. in this species, gene amplifica ... | 2003 | 12886275 |
| spatial and temporal genetic variability in french populations of the peach-potato aphid, myzus persicae. | the peach-potato aphid, myzus persicae (sulzer), has a complex reproductive mode in which some lineages reproduce by continuous parthenogenesis, whereas others reproduce sexually once a year. the climate is thought to act directly on the reproductive mode, because sexual eggs are the only form that can resist frost in cold regions. sexual reproduction necessitates an obligatory host alternation that may result in long-distance dispersal. here, we examined the genetic variability at seven microsa ... | 2003 | 12886281 |
| aphid-induced defense responses in mi-1-mediated compatible and incompatible tomato interactions. | the tomato mi-1 gene confers resistance to three species of root-knot nematode and potato aphid. we studied changes in expression of jasmonic acid (ja)- and salicylic acid (sa)-dependent defense genes in response to potato and green peach aphids. we determined changes in three pr proteins, lipoxygenase and proteinase inhibitors i and ii transcripts, locally and systemically in both compatible and incompatible interactions in tomato. transcripts for pr-1 were detected earlier and accumulated to h ... | 2003 | 12906114 |
| incidence of insecticide resistance alleles in sexually-reproducing populations of the peach-potato aphid myzus persicae (hemiptera: aphididae) from southern france. | intensive chemical treatments have led to the development of a number of insecticide resistance mechanisms in the peach-potato aphid myzus persicae (sulzer). some of these mechanisms are known to be associated with negative pleiotropic effects (resistance costs). molecular and biochemical methods were used to determine the genotypes or phenotypes associated with four insecticide resistance mechanisms in single aphids from sexually-reproducing populations in southern france. the mechanisms consid ... | 2003 | 12908914 |
| [field efficacy of emulsifiable suspensions of beauveria bassiana conidia for control of myzus persicae population on cabbage]. | the conidial powder of beauveria bassiana sg8702 produced using a diphasic fermentation technology was formulated into emulsifiable suspension i (ei-i, > 10(10) conidia.ml-1) and es-ii, which resulted from adding 1% (w/v) of imidacloprid 10% wp to the es-i. three dilutions (10(7), 10(6), and 10(5) conidia.ml-1 of each were sprayed onto cabbage plants for control of the green peach aphid myzus persicae population in field plots in kunming, yunnan during july 2001. each dilution and a water spray ... | 2003 | 12920898 |
| cloning and molecular characterization of a novel lectin gene from pinellia ternata. | the full-length cdna of pinellia ternata agglutinin (pta) was cloned from inflorescences using race-pcr. through comparative analysis of pta gene (pta) and its deduced amino acid sequence with those of other araceae species, pta was found to encode a precursor lectin with signal peptide and to have extensive homology with those of other araceae species. pta was a heterotetrameric mannose-binding lectin with three mannose-binding boxes like lectins from other araceae and amaryllidaceae species. s ... | 2003 | 12974620 |
| the green peach aphid on tobacco in the dominican republic. | 1952 | 13028257 | |
| feeding behaviour of the aphids myzus persicae and brevicoryne brassicae, studied with radiophosphorus. | 1953 | 13051326 | |
| evidence that potato virus y is carried near the tip of the stylets of the aphid vector myzus persicae (sulz.). | 1955 | 13270154 | |
| does the aphid myzus persicae (sulz.) imbibe tobacco mosaic virus? | 1956 | 13325405 | |
| three more viruses borne at the stylet tips of the aphid myzus persicae (sulz.). | 1957 | 13437229 | |
| studies on the behavior of potato leaf roll and other viruses in the body of their aphid vector myzus persicae (sulz.). | 1958 | 13581530 | |
| characterization of a new densovirus infecting the green peach aphid myzus persicae. | a new icosahedral dna virus was isolated from aphids (myzus persicae) that showed abnormal growth and development. the purified virus particles have a diameter of 20 nm and contain a single-stranded dna molecule of approximately 5.7 kb. the viral particles are composed of five structural proteins (92, 85, 68, 64, and 57 kda). as the main biophysical properties of this virus are similar to those of the members of the genus densovirus it was tentatively named myzus persicae densovirus (mpdnv). a p ... | 2003 | 13678707 |
| [on the functional morphology of the salivary glands of homoptera. iv. the secretory ducts of the salivary glands of myzus persicae (sulz.), aphididae]. | 1960 | 13771645 | |
| re-evaluation of retention of the beet yellows virus by green peach aphids under fasting and feeding conditions. | 1961 | 13774366 | |
| [on functional morphology of salivary glands of homoptera. report 3: the accessory gland of myzus persicae (sulz.), aphididae]. | 1960 | 13786197 | |
| short-term probing behavior of myzus persicae (sulzer) as affected by fasting, anesthesia, and labial manipulation. | 1963 | 13979776 | |
| the purification of viruslike particles from the aphid myzus persicae. | 1965 | 14295230 | |
| [on the functional morphology of the salivary glands of homoptera. part i. the chief cells of the principal gland of myzus persicae (sulz.), aphididae]. | 1960 | 14423192 | |
| new use of broomcorn millets for production of granular cultures of aphid-pathogenic fungus pandora neoaphidis for high sporulation potential and infectivity to myzus persicae. | glutinous broomcorn millets from the crop panicum miliaceum were first used as substrate to produce granular cultures of pandora neoaphidis, an obligate fungal pathogen specific to aphids. carrying a water content of 36.5% after being steamed in a regular autoclaving procedure, millet grains of each 15 g (dry weight) in a 100-ml flask were mixed with 3 ml modified sabouraud dextrose broth containing half a mashed colony of p. neoaphidis grown on egg yolk milk agar and then incubated at 20 degree ... | 2003 | 14592724 |
| dose-response relationship in lethal and behavioural effects of different insecticides on the parasitic wasp aphidius ervi. | neurotoxic insecticides are widely used for crop protection and behavioural perturbations can be expected in surviving beneficial insects, including parasitoids of pest insects. the present study aims to investigate the relationship between the dose of insecticide parasitoids have been exposed to, and the subsequent ability of these parasitoids to respond to host-related cues. a four-armed olfactometer, a design widely used to observe orientation responses in various insects and parasitoids in p ... | 2004 | 14599507 |
| transformation of tobacco with genes encoding helianthus tuberosus agglutinin (hta) confers resistance to peach-potato aphid (myzus persicae). | the effects of the hta gene encoding helianthus tuberosus agglutinin (hta) on an insect in the order homoptera were investigated. homologous cdnas of hta-a, hta-b, hta-c and hta-d with camv35s as promoter were introduced into tobacco via agrobacterium tumefaciens. southern blot results showed that the exogenous hta gene was inserted into the genome of host plants, and northern blot analysis confirmed that hta was expressed in transgenic plants. a bioassay with peach-potato aphid (myzus persicae) ... | 2003 | 14601659 |