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blastocystis hominis: clinical and therapeutic aspects. 19883176148
a survey of gastrointestinal parasites in ogun state, southwest nigeria.to study the epidemiology of intestinal parasites in ogun state, nigeria, 479 stool specimens were examined at a hospital in abeokuta during the rainy season in june 1986. intestinal helminths and/or protozoa were present in 297 samples (62%). 41.1% of the specimens showed a single infection, 34% showed double, 20% triple and 5% quadruple infection. the most commonly found worm was ascaris lumbricoides (in 40% of specimens), followed by trichuris trichiura (23.2%), hookworms (19.2%), strongyloid ...19883178337
enzyme immunoassay for detection of giardia lamblia cyst antigens in formalin-fixed and unfixed human stool.an antigen-capture enzyme-linked immunosorbent assay employing rabbit and mouse antisera to giardia lamblia cyst antigens was developed for the diagnosis of giardia infection through detection of g. lamblia-specific stool antigens in cell-free aqueous eluates of human stool. this is the first report of the use of anti-cyst antibodies in an enzyme immunoassay for g. lamblia. the assay gave a positive result with 54 of 59 stools from patients with symptomatic, clinically diagnosed giardiasis, givi ...19883183015
freeze-fracture study of blastocystis hominis.the ultrastructure of blastocystis hominis was investigated by the freeze-fracture method. freeze-fracture replicas of the membranes of b. hominis and its organelles were studied with special regard to the density and distribution of the intramembranous particles (imp's). on all membrane replicas, the concentration of imp's on the protoplasmic face (p face) invariably was greater than on the exoplasmic face (e face). on the p face, imp's were heterogeneously distributed in dense aggregates, alte ...19883199336
[enteritis caused by blastocystis hominis]. 19883236933
[blastocystis hominis: a new pathogenic agent?]. 19883255133
intestinal parasites in children with diarrhea in el salvador.in a study of parasitological prevalence in el salvador, stool specimens of 210 children with diarrhea were examined for intestinal parasites. in 104 cases (49%), intestinal helminths and protozoa were found. 53.4% of the specimens showed single infestation, 31.7% showed double. 3% triple and 1% quadruple infestation. of the helminths, 31% were trichuris trichiura, 18% ascaris lumbricoides and 5% hymenolepis nana. of the pathogenic protozoa, 7% were identified as giardia intestinalis, 6% entamoe ...19883262555
association of blastocystis hominis with human disease. 19883356799
presumptive evidence for blastocystis hominis as a cause of colitis.a patient with persistent diarrhea was found to have biopsy-proved colitis with large numbers of the protozoan blastocystis hominis present in stool. extensive evaluation failed to reveal any other potential etiologic agent of acute colitis. following treatment with a course of metronidazole, the patient became asymptomatic, b hominis was no longer present in stool, and results of a repeated biopsy were normal. these observations are consistent with the role of b hominis as a gastrointestinal pa ...19883365077
blastocystis hominis, brumpt 1912: are there different stages or species? 19883387411
etiology of diarrhea among travelers and foreign residents in nepal.a bacterial pathogen was isolated from 47% of 328 expatriate patients with diarrhea seen at two medical clinics in nepal in 1986. enterotoxigenic escherichia coli (24%), shigella (14%), and campylobacter species (9%) were isolated most frequently. enteroinvasive and adherence factor-positive e coli were isolated from 2% and 1% of patients, respectively. giardia lamblia was detected in 12% of patients, rotavirus in 8%, and cryptosporidium and entamoeba histolytica each in 5%. blastocystis hominis ...19883404637
epidemiology and clinical features associated with blastocystis hominis infection.a retrospective chart review was performed on 100 patients infected with blastocystis hominis (bh) and 50 randomly selected age and sex matched controls to examine the clinical and epidemiologic features associated with this organism. the finding of greater than 5 bh per oil immersion field (1,000 x) was significantly associated with acute presentation of symptoms but was not predictive of the presence of gastrointestinal symptoms. of patients infected with bh, 57.5% had recently travelled to th ...19873449317
cryptosporidiosis in ogun state, south-west nigeria.during the period of one month in the rainy season in ogun state in south-west nigeria 479 stool samples (420 of which were diarrhoea stool samples) were examined for cryptosporidiosis. oocysts were detected in 2.3% of all stools, in 2.6% of diarrhoeal stools and in 5.3% out of 150 children with diarrhoea. cryptosporidium was the sole pathogen detected in six of 11 cases. in addition to cryptosporidia also entamoeba histolytica, blastocystis hominis, giardia lamblia, ascaris lumbricoides, and tr ...19873602839
diarrhea due to blastocystis hominis: an old organism revisited.we have reported a case of diarrhea caused by blastocystis hominis, an intestinal protozoan parasite of man. the organism is present in small numbers in up to one fifth of stool samples in hospitalized patients, but is associated with diarrhea in only heavily infested patients. typical symptoms include diarrhea, crampy abdominal pain, nausea, vomiting, low-grade fever, gas, malaise, and chills. fecal leukocytes are occasionally seen. the pathophysiologic mechanism of the diarrhea is not clear. n ...19873603119
[prevalence of blastocystis hominis in refugees]. 19873622300
light-microscopical appearance and ultrastructure of blastocystis hominis, an intestinal parasite of man.a study on blastocystis hominis was undertaken for the purpose of clarifying the morphology of the organism using the following techniques; giemsa, heidenhain iron hematoxylin, and dapi stains, phase-contrast microscopy, and transmission- and scanning-electron microscopy. microscopic examinations of the lumen fluids aspirated at the endoscopical examination revealed the habitation of b. hominis in the lower ileum and cecum of a patient. when examined light-microscopically, organisms from stool m ...19873660977
blastocystis hominis: evidence for human pathogenicity and effectiveness of metronidazole therapy.1. clinical symptoms and oral treatment with metronidazole were studied in 103 patients with pure infections by blastocystis hominis. 2. the results showed that excessive flatulence is the chief gastrointestinal symptom associated occasionally with diarrhoea and abdominal cramps. all the patients showed good responses with treatment of metronidazole and 74 patients whose stools were reexamined 1-2 months after the treatment demonstrated no signs of infections. 3. it is concluded that b. hominis ...19873665198
blastocystis hominis: pathogen or fellow traveler?blastocystis hominis, an intestinal organism of uncertain taxonomic position, has long been considered nonpathogenic. some recent studies suggest, however, that it may be associated with diarrhea and may respond to treatment with iodoquinol or metronidazole. to investigate this possibility, we identified 148 persons whose stools contained this organism. of this number, 32 had at least 6 stool examinations performed. twenty-seven of the 32 persons were later found to have greater than or equal to ...19863766850
association of blastocystis hominis with signs and symptoms of human disease.purged stools from 389 patients were evaluated microscopically for the presence of blastocystis hominis. a total of five or more b. hominis cells per 40x field were observed in 43 patients (11%), and b. hominis was the only intestinal parasite present in 23 (6%) of these patients. of the 23 patients, 19 had symptoms which included abdominal discomfort (15 patients), anorexia (10 patients), diarrhea (9 patients), and flatus (9 patients). the remaining four patients were asymptomatic. the proporti ...19863771743
[a case from practice (66). patient: e.k., born oct. 10, 1948, housewife (blastocystis hominis infection)]. 19863787022
parasitological observations of chronic diarrhoea in suspected aids adult patients in kinshasa (zaire).the parasitological profile of chronic diarrhoea in 46 zairian adults suspected of aids demonstrated that the frequency of protozoa was five times higher than that of helminths; 86% of the protozoa were sporozoa: isospora belli was the most frequent (19%), followed by cryptosporidium isolated for the first time in zaire (8%) and blastocystis hominis (2%). 37 of the 46 patients were immunodeficient.19863787692
etiology and epidemiology of diarrheal diseases in the united states.accurate data on the frequency of acute diarrheal illness and the distribution of pathogens are not available for several reasons, including the facts that only a small fraction of cases come to the attention of physicians and that available diagnostic tests establish an etiology in only about half of these. in a survey of three groups of patients in a community (upper-middle class and lower class outpatients and hospitalized infants), a possible cause was found in fewer than 20 percent of outpa ...19853893118
cytochrome-free mitochondria of an anaerobic protozoan--blastocystis hominis.isolated mitochondria of the anaerobic protozoan blastocystis hominis were subjected to spectral analysis, color, catalase, and peroxidase tests and found to be completely negative for cytochrome enzymes, catalase, and peroxide. based on the absence of cytochrome enzymes, the possible evolution of b. hominis mitochondria from anaerobic bacteria is postulated.19863959009
afebrile diarrhea and blastocystis hominis. 19854014917
ultrastructural aspects of blastocystis hominis strain resistant to antiprotozoal drugs. 19854038050
blastocystis hominis infection presenting as recurrent diarrhea. 19854038860
ketoconazole and resistant blastocystis hominis infection. 19854040725
blastocystis hominis enteritis. 19854047965
blastocystis hominis in kathmandu, nepal. 19854058539
a comparison of the outer layers of blastocystis hominis with the cyst walls of certain common intestinal protozoa. 19714329022
ultrastructure of blastocystis hominis. 19734360331
blastocystis hominis: axenic cultivation. 19744369819
[blastocystis hominis brumpt, 1912, (phycomycetes) in the female genital tract]. 19724537319
studies of blastocystis hominis. 19734690644
[occurrence of blastocystis hominis in turkey and morphological characteristics of this organism (apropos of a case)]. 19695399002
protozoan characteristics of blastocystis hominis. 19676058384
blastocystis hominis: a neglected cause of diarrhoea? 19846143904
clinical relevance of blastocystis hominis. 19846144939
[blastocystis hominis: morphological study using optical and electron microscopy].blastocystis hominis is a member of the phylum protozoa as described by zierdt et al. despite the complexity of its yet not well defined biological cycle, blastocystis was found to possess all the structures characterizing protozoans. the physiochemical and biological growth conditions are those of many protozoans. the high degree of cell differentiation is probably due to the existence of two reproduction modes.19836368020
in vitro response of blastocystis hominis to antiprotozoal drugs.ten antiprotozoal drugs were tested in vitro against four axenic strains of the intestinal parasite blastocystis hominis. inhibitory drugs in order of effectiveness were emetine, metronidazole, furazolidone, trimethoprim sulfamethoxazole, 5-chloro-8-hydroxy-7-iodo-quinoline (entero-vioform), and pentamidine. moderately inhibitory were two quinolines other than iodochlorhydroxquin. these were chloroquine and 5,7-diiodo-8-hydroxy-quinoline (floraquin). diloxanide furoate was not inhibitory. paromo ...19836631776
ultrastructural aspects of blastocystis hominis. 19836687040
[what should one believe about blastocystis hominis?]. 19816819414
blastocystis hominis in a pig-tailed macaque: a potential enteric pathogen for nonhuman primates.large numbers of blastocystis hominis were found in fecal samples from a pig-tailed macaque (macaca nemestrina) with chronic diarrhea that was refractory to conventional therapy. the animal's condition improved and fecal samples showed minimal numbers of blastocystis hominis following therapy with an antiprotozoal agent. this organism, recently reclassified as a protozoa, appeared to be causally related to the diarrhea. the cytologic and ultrastructural features of the monkey blastocystis were c ...19807191935
generation time and growth rate of the human intestinal parasite blastocystis hominis.blastocystis hominis, an anaerobic intestinal protozoan parasite of man, has a generation time (gt) in axenic culture of 8.5-19.4 h. depending on the strain tested. average gt of the eight strains was 11.7 h. zero growth time cell counts of 5.0 x 10(5)/ml to 2.0 x 10(6)/ml rose in 3-5 days to 1 x 19(7) cells/ml. the gt was determined for the 24-hr period during which the most rapid growth occurred; about 2% of the b. hominis cells were in division during this time. division under the culture con ...19817198685
blastocystis hominis: improved axenic cultivation. 19817199211
[frequency and significance of parasitic infections in refugees from vietnam (author's transl)].in the course of the medical care which is provided for the refugees from southeast asia 100 persons were tested for intestinal parasites (helminths and protozoa). in addition serum samples were obtained from 72 of these and tested in several tests (cft, fat, iha and sft) using antigens of entamoeba histolytica, leishmania donovani, toxoplasma gondii, schistosoma spp., fasciola hepatica, echinococcus spp., trichinella spiralis and litomosoides carinii. in 49 persons infections with one, two or ...19807395245
elucidation of the life cycle of the intestinal protozoan blastocystis hominis.this paper elucidates the status of the different morphological forms of blastocystis and reports the existence of thin- and thick-walled cysts in b. hominis on the basis of current experimental evidence. it is suggested that the thin-walled cysts are autoinfectious, leading to multiplication of the organism in the intestinal tract. the thick-walled cysts are responsible for external transmission via the faecal-oral route. a life cycle for b. hominis is postulated on the basis of these findings.19957501648
serum antibody detected by fluorescent antibody test in patients with symptomatic blastocystis hominis infection. 19957501905
morphological diversity of blastocystis hominis in sodium acetate-acetic acid-formalin-preserved stool samples stained with iron hematoxylin.the objective of this investigation was to study the morphological characteristics of blastocystis hominis in sodium acetate-acetic acid-formalin-preserved stool samples. routinely processed samples were examined for morphological detail, including size, shape, nuclear detail, and central body characteristics. morphological findings revealing the importance of recognizing b. hominis in the diagnostic laboratory are described.19947510311
differentiation of the various stages of blastocystis hominis by acridine orange staining.acridine orange staining differentiates the cystic and the central body forms of blastocystis hominis and offers a very convenient and easy method to observe the internal structure of the parasite. acridine orange stains the nuclei and the central body of the rounded vacuolar forms of the parasite bright and dull green, respectively. the colour changes to yellow and then to flaming red-orange when the rounded central body forms of the parasite become cystic.19947521862
histochemical detection of carbohydrates of blastocystis hominis.the carbohydrates of blastocystis hominis were detected by histochemical techniques using light and electron microscopy. b. hominis, fixed with various fixatives, followed by treatment with detergents, were stained with periodic acid-schiff (pas) or alcian blue (ab). intense pas reactions were observed in cells fixed with glutaraldehyde or 1/2 karnovsky fixative. the cells fixed with other fixatives showed weak or no reactions with pas staining. similar results were seen in the case of ab stain. ...19957537145
light and electron microscopic localization of lipids in blastocystis hominis.localization of the lipid of blastocystis hominis was histochemically demonstrated. b. hominis was fixed with glutaraldehyde, treated with detergents, and stained with sudan black b or nile blue. the central vacuole of b. hominis stained with sudan black b showed many black droplets with great variation in staining intensity. in nile blue staining, the central vacuole showed many pinkish granules or homogeneous blue reactions. these results indicated the presence of neutral lipids and/or acidic ...19957544390
[morphology of blastocystis hominis in feces and evaluation of parasitological methods].a study of the morphology of blastocystis hominis in stool in ninety-four cases humans is described as central body, ameba and granular form were found as previously described. in addition a "globulose" form as a variation of granular form is first described. the central body form (96.8%) was the most abundant form. three parasitological methods as direct microscopical examination sample with saline solution 0.85%, lugol, sudan iii, stained with quensel, iron hematoxylin and culture are evaluate ...19947557277
ultrastructure of blastocystis hominis cysts.blastocystis hominis cysts were concentrated from human faeces by repeated washing in distilled water and centrifugation on ficoll-paque. this procedure gave a concentrated suspension of cysts without yielding any non-cystic forms. the cysts were examined by both transmission (tem) and scanning electron microscopy (sem). cysts were surrounded by a fibrillar layer that appeared porous in sem. many naked cysts without the fibrillar layer were also visible in sem. they were variable in shape but mo ...19957567903
prevalence of intestinal parasitic infection in five farms in holambra, são paulo, brazil.a parasitological survey was carried out on 222 inhabitants of five farms in holambra, located 30 km north of campinas, são paulo, brazil, on october 1992. approximately 70% of the inhabitants were found to be infected with at least one species of intestinal parasite. the positive rates of 6 helminths and 7 protozoan species detected are as follows: 5.4% ascaris lumbricoides; 8.6% trichuris trichiura; 19.8% necator americanus; 10.4% strongyloides stercoralis; 1.4% enterobius vermicularis; 0.9% h ...19957569635
blastocystis hominis and traveler's diarrhea. 19957578716
is there any reason to continue treating blastocystis infections? 19957578717
is blastocystis hominis a cause of diarrhea in travelers? a prospective controlled study in nepal.although the pathogenicity of blastocystis hominis has been extensively debated in the medical literature, controlled studies of the association between b. hominis and diarrhea are lacking. we conducted a case-control study among expatriates and tourists in kathmandu, nepal, in which we compared the prevalence of the organism among patients with diarrhea to that among a control group without diarrhea. b. hominis was detected in 56 (30%) of 189 patients with diarrhea, compared with 40 (36%) of 11 ...19957578767
bacteria, viruses, yeasts and protozoans associated with diarrheal disease in singapore.labile toxin producing enterotoxic e. coli (etec) were the commonest pathogen isolated from diarrheal stools of hospitalized children (21%) and adults (26%) in singapore. salmonellas ranked a close second in children (19%). other bacterial pathogens were isolated from less than 5% of subjects. blastocystis hominis was detected in 4.3% of diarrheal stools when a simple sedimentation technique was used. cryptosporidium was not detected at all. an analysis of yeast counts in smears of diarrheal and ...19957603751
[prevalence of infection by intestinal helminths and protozoa in school children from a coastal locality in the province of valdivia, chile].during july-august 1989, 219 coprological samples from primary school children from niebla and los molinos localities (39 degrees 52's, 73 degrees 26'w) in the coast of province of valdivia, chile, were analysed. prevalence % (in parentheses) of infection by intestinal protozoa and helminths were the followings: entamoeba histolytica: (18.0), entamoeba coli (34.0), endolimax nana (34.4), iodamoeba buetschlii (7.4), blastocystis hominis (64.3), giardia intestinalis (27.9) chilomastix mesnili (0.8 ...19947654292
morphology of the cyst of blastocystis hominis. 19947667736
a comparison of direct microscopy with culture for the diagnosis of blastocystis hominis. 19947667737
practice guidelines for ordering stool ova and parasite testing in a pediatric population. the alberta children's hospital.a comprehensive utilization review was done of all stool ova parasite examinations performed at a pediatric hospital during a 3-year period from june 1, 1989 to july 1, 1992. a total of 2,652 stool specimens were surveyed from 1,532 children. forty-one percent (1,081) of the workload was from inpatients, 37% (976) was from emergency room (er) visits/other outpatients, and 22% (233) was from patients attending the gastroenterology (gi) clinic. the prevalence of enteric parasites in hospitalized c ...19957677114
biochemical characterisation of human isolates of blastocystis hominis.sds-page and iso-enzyme analysis of 11 human isolates of blastocystis hominis revealed at least two variants with different polypeptide patterns and two zymodemes, respectively. this is the first iso-enzyme and the second protein analysis to indicate strain differences in b. hominis.19957707340
[comparison of intestinal parasite infestation indexes among hiv positive and negative populations].this study was carried out on two groups of patients treated at the josé de san martín clinical hospital. one group was composed of 82 hiv-seropositive patients with no signs of diarrhea, and another one of 300 patients, not suspected of hiv infection, was considered as "control population". stool samples were collected from each patient and examined for intestinal parasites. these determinations were carried out at the department of clinical biochemistry, in the same hospital. three specimens w ...19947715428
blastocystis hominis infection, a cause of human diarrhea.blastocystis hominis has long been described as a non pathogenic protozoan parasite until recently when claims have been made that it can result in pathogenic conditions. of the 729 stool samples (614 from survey and 115 from pediatric wards) examined, 18.1% of them were found to be positive for one or more intestinal protozoan cyst. the commonest was giardia intestinalis (8.4%) followed by entamoeba coli (7.1%) and entamoeba histolytica (5.1%) in the normal children without symptoms of diarrhea ...19947777913
tubulovesicular elements in blastocystis hominis from the caecum of experimentally-infected rats.by transmission electron microscopy (tem), tubulovesicular elements were seen in blastocystis hominis obtained from the caecum of experimentally-infected rats. these appeared to arise from the peripheral cytoplasm and were rounded, oval or elongate in sections. it is suggested that these elements form a network for transfer of nutrients to the periphery during the process of encystation.19957797363
a concentration technique for obtaining viable cysts of blastocystis hominis from faeces. 19947799511
a multiple fission-like mode of asexual reproduction in blastocystis hominis.a non-axenic and an axenic isolate of blastocystis hominis have been induced to form cysts in vitro using an encystation medium. the morphology of the parasite at different time points was observed by scanning electron microscopy. in day-2 cultures the cysts were spherical and had a non-uniform, coarse outer surface around the body. a deep, pore-like opening was seen in some of the parasites. most of the cysts from day-4 and day-6 cultures ruptured, revealing small, uniformly sized spherical bod ...19947809004
isolation of blastocystis hominis from sewage. 19947825017
intestinal colonization of symptomatic and asymptomatic schoolchildren with blastocystis hominis.a study of single stool specimens was done to determine the prevalence of intestinal parasites among 1,000 primary school children. a questionnaire was completed by each child's parents. specimens were examined by using wet-mount preparation, formaline-ether concentration, and sheather's flotation technique. trichrome and acid-fast stains were done. blastocystis hominis was observed in 203 (20.3%) of the specimens examined, and 175 specimens contained this organism in the absence of other pathog ...19947852590
a study of the karyotypic patterns of blastocystis hominis by pulsed-field gradient electrophoresis. 19947855129
enzyme-linked immunosorbent assay for detection of serum antibody to blastocystis hominis in symptomatic infections.an enzyme-linked immunosorbent assay was devised in order to search for antibodies against blastocystis hominis in infected humans. reaction proteins were obtained from washed, axenic b. hominis cells, as sonicate. sonicate was diluted to provide 17 and 34 micrograms of protein per well. dilutions of patients' sera were applied, followed by phosphatase-conjugated goat anti-human serum and phosphatase substrate. color was measured at 405 microns wavelength. immunoglobulin g antibodies to high tit ...19957876972
diagnostic methods for intestinal parasites in southern iraq with reference to strongyloides stercoralis.three hundred and thirty-two stool samples were examined for the presence of intestinal parasites including strongyloides stercoralis. each sample was processed and examined by direct smear, formalin-ether and harada and mori culture methods. nine parasites were recovered from patients attending basrah teaching hospital, southern iraq during 1989. the prevalence rate of infection was 64.2%. it was higher in rural (74.2%) than in urban (57.5%) region (p < 0.01). sex distribution was 120 (36.1%) m ...19937939941
touch cytology. a quick, simple, sensitive screening test in the diagnosis of infections of the gastrointestinal mucosa.to assess the role of touch cytology (imprint from endoscopic biopsy specimens) in the diagnosis of mucosal infections of the gastrointestinal tract, we reviewed all records and specimens of patients seen during a 30-month period. touch cytology was performed by rolling biopsy specimens on glass slides. after air fixation, a rapid staining method similar to may-grünwald-giemsa was used. the following infections and pathogens were diagnosed (in decreasing order of frequency): helicobacter pylori ...19947979897
[blastocystis hominis as a rare arthritogenic pathogen. a case report].blastocysts hominis, a protozoon considered to be a nonpathogen intestinal commensal, is now discussed to be a cause of intestinal infection under certain circumstances, e.g., immunosuppression. there are two published cases of blastocysts infection complicated by arthritis which was classified as "reactive" in one case, "infectious" in the other. we report a third case: a 46-year-old female patient developed a chronic diarrhea and oligoarthritis some days after returning from a trip to senegal. ...19948023590
intestinal parasitism in the united states: update on a continuing problem.to document patterns of intestinal parasitism in the united states, we analyzed results of 216,275 stool specimens examined by the state diagnostic laboratories in 1987; parasites were found in 20.0%. percentages were highest for protozoans: giardia lamblia (7.2%), entamoeba coli and endolimax nana (4.2% each), blastocystis hominis (2.6%), and entamoeba histolytica (0.9%). the most commonly identified helminths were nematodes: hookworm (1.5%), trichuris trichiura (1.2%), and ascaris lumbricoides ...19948024063
incidence of blastocystis hominis in patients with diarrhoea.we studied the occurrence of the parasite blastocystis hominis in 1066 stool specimens from patients with diarrhoea, and investigated the relationship between the presence of b. hominis in the faeces and the age of patients. the parasite was recovered from 3.7% samples, but as the sole species of micro-organism in the stool it was recovered from 1% samples. there was no statistically significant difference in the number of b. hominis-positive stools between the younger and the older patients (p ...19948034994
blastocystis hominis infection and intestinal injury.blastocystis hominis is an enteric protozoan associated with clinical illness. to determine the prevalence of intestinal injury in patients with b. hominis infection, the authors prospectively evaluated 18 patients with b. hominis infection by endoscopy and a test of intestinal permeability. seventeen patients had gastrointestinal symptoms. colonic mucosa appeared normal by lower endoscopy in 12 of 13 patients, and was friable slightly in 1. duodenal mucosa was normal by upper endoscopy in nine ...19948042662
invasive blastocystis hominis infection in a child. 19948044274
[intestinal parasitosis in the 90s: new microorganisms in new patients]. 19948059032
ultrastructural changes during in vitro encystment of blastocystis hominis.the morphological changes occurring in blastocystis hominis at different time points following in vitro encystment were studied by electron microscopy. the following stages of the parasite were sequentially seen: (a) the amoebic form, which was irregular in shape, with a majority of the organelles being concentrated at the condensed cytoplasmic region; (b) the pre-cystic form, which was rounded and had an electron-dense material forming a homogeneous wall around the central body; and (c) the cys ...19948073021
blastocystis hominis: occurrence in children and staff members of municipal day-care centers from botucatu, sáo paulo state, brazil.to study the frequency of blastocystis hominis among healthy individuals, feces were collected from 153 children and 20 staff members of some municipal day-care centers. three separate stool specimens of each individual were processed by lutz and faust methods. from 173 studied individuals, 60 (34.7%) showed b. hominis, frequently in association with other intestinal parasites and/or commensals. b. hominis was found mainly in adults and children between 36 and 72 months old. all positive cases w ...19938107605
[blastocystis infection in symptomatic patients at the regional hospital of temuco, chile].at the regional hospital of temuco, 670 adult and children patients, with intestinal complaints, were studied to determine the prevalence of infection by enteroparasites or commensals in a general way, and by blastocystis hominis in a particular attempt. a coproparasitological study was carried out using a modified telemann method. infection by parasites and/or commensals was demonstrated in 446 (66.6%) patients. the most frequent agent was b. hominis (35.8%) followed by giardia intestinalis (22 ...19938110370
[intestinal parasite infections in rural students of chiloé archipelago, x region, chile].in order to contribute to the knowledge of intestinal parasite infection a survey was performed in 462 primary school children from eight rural localities sited in two islands of the chiloe archipelago. stool samples and perianal scotch tape scrapings for intestinal protozoa and helminths were microscopically examined. the prevalence rates of the main species found were: ascaris lumbricoides 43.3%, trichuris trichiura 26.8%, enterobius vernicularis 20.1%, entamoeba histolytica 5.6%, giardia inte ...19938110371
coccidian/cyanobacterium-like bodies as a cause of diarrhea in australia.coccidian/cyanobacterium-like bodies (clbs) were detected in the stool specimens of 5 australian patients over a 6 mth period from july to december, 1992. four of the 5 patients had recent travel history to a neighbouring overseas destination, including papua new guinea and bali, indonesia. these 4 patients had clinical presentations of acute onset, recurrent diarrhea which was prolonged and accompanied by weight loss, anorexia and lethargy. these symptoms are consistent with reports from other ...19938165002
four serologically different groups within the species blastocystis hominis.61 strains of blastocystis hominis were isolated from 340 stool specimens coming from 140 patients with intestinal symptoms and from 200 healthy persons. the overall female to male ratio was 1.9 to 1. classification of the 61 isolates of b. hominis was determined with the aid of the immunodiffusion assay. four serologically different groups could be identified. their frequency ratio in the population studied was 39.5%:39.5%:18%:3%. group 3 was found more often than the other three groups in stoo ...19948167435
lipid biosynthesis by axenic strains of blastocystis hominis.axenic strains of blastocystis hominis incorporated 32p, added to the medium as orthophosphate, into a number of phospholipids, including sphingomyelin, cardiolipin, phosphatidic acid, the phosphoglycerides of choline, ethanolamine, serine, and inositol and some other minor phospholipids. radioactive palmitate and glycerol provided in the growth medium introduced radiolabel into diacylglycerols, triacylglycerols, and all major phosphoglycerides found in the organism. palmitate is a major fatty a ...19948205379
morphology of blastocystis sp. isolated from circus animals.blastocystis sp. is reported for the first time from faecal samples collected from a camel, a llama, a highland bull and a lion in a travelling circus. fresh faecal specimens were examined by light and electron microscopy, and vacuolar and cyst forms of similar morphology were present in all three ungulates. these cells were smaller than cultured vacuolar cells of blastocystis hominis isolated from humans and contained only a single vacuole in comparison to the multivacuolar cell found in fresh ...19938225774
urticaria by blastocystis hominis. successful treatment with paromomycin.urticaria and angioedema are easily recognized disorders, but in at least 70 percent of individuals, chronic episodes of urticaria are of unknown causes. we present 10 cases of chronic urticaria associated parasitation by blastocystis hominis. this parasite has not been previously related with urticaria. both intestinal parasitation as well as urticaria responded successfully to paromomycin sulfate.19938237719
evidence of an epidemic of blastocystis hominis infections in preschool children in northern jordan.blastocystis hominis is now gaining acceptance as an agent of human intestinal disease. a case-control study of the cause of gastroenteritis in children less than 6 years old was conducted. a total of 500 stool specimens were examined by wet mount preparation, formalin-ether concentration, sheather's sugar flotation technique, and permanent stains when necessary. b. hominis was found in 63 (25%) of 250 stool specimens of the cases examined; 38 (15%) of these specimens contained this parasite alo ...19938253970
amebiasis and "nonpathogenic" intestinal protozoa.infection with single or multiple species of intestinal protozoa is common in humans and can result in either asymptomatic colonization or symptoms of intestinal disease. entamoeba histolytica serves as a paradigm for invasive colonic protozoal infection. the key to diagnosis and treatment of amebiasis is knowledge of the epidemiologic risk factors and clinical manifestations, a rational approach to diagnosis, and an understanding of the sites of action and uses of anti-amebic drugs. this knowle ...19938254155
intestinal parasitic infections in campalagian district, south sulawesi, indonesia.intestinal parasitic infections were surveyed in the inhabitants of 3 coastal and 2 inland villages of campalagian district, south sulawesi, indonesia, in july 1992. a total of 398 fecal samples were examined by using kato-katz thick smear, harada-mori culture and agar-plate culture techniques. protozoan cysts were examined by formalin ether concentration technique on 380 fecal samples. soil-transmitted helminth infections were highly prevalent with the overall positive rates as follows: ascaris ...19938266235
axenic culture of blastocystis hominis in iscove's modified dulbecco's medium. 19938278347
diagnosis by faecal culture of dientamoeba fragilis infections in australian patients with diarrhoea.this paper reports the first survey in australia to use faecal culture to detect dientamoeba fragilis in patients with diarrhoea. of 3 different protozoal culture media evaluated on a case of known infection, modified boeck & drbohlav's medium was the most suitable. the organism could be grown from faeces stored for up to 24 h at room temperature, but for only 10 h at 4 degrees c. culture was then used, in combination with microscopy of smears fixed with polyvinyl alcohol and trichrome-stained, ...19938337717
prevalence of intestinal protozoans in french patients infected with hiv.to assess the prevalence of intestinal protozoans in french hiv-infected patients, stool samples, duodenojejunal biopsies, and/or colorectal biopsies from 81 patients were studied for parasites, viruses, and bacteria. pathogens were found in 70.6% of aids patients with diarrhea or malabsorption. the respective prevalence of protozoa in aids patients with diarrhea was cryptosporidium sp.: 37.3%, blastocystis hominis: 13.7%, giardia intestinalis: 5.8%, isospora belli: 2%, enterocytozoon bieneusi: ...19938340892
in vitro encystment and experimental infections of blastocystis hominis.cultures of blastocystis hominis were induced to encyst using three encystation media: (a) an encystation medium (em) comprising yeast extract in buffered saline containing 50% horse serum, (b) an encystation medium (cem) comprising em conditioned with bacterial soluble products and (c) an encystation medium (tem) containing 0.5% trypticase in em. two isolates of b. hominis were studied, an axenized isolate c and a non-axenized isolate ms. in em, isolate c did not encyst, whereas 6.1% of isolate ...19938415557
blastocystis hominis and blastocystosis (zierdt-garavelli disease). 19938428021
prevalence and characteristics of blastocystis hominis infection in children.blastocystis hominis, a protozoan whose pathogenicity has been questioned, is sometimes found in the human gastrointestinal tract. we sought to determine the prevalence of blastocystis in stool and to characterize clinical features of infection with blastocystis in children. forty-six (3%) of 1,736 patients undergoing fecal microscopy at children's hospital of pittsburgh between january 1, 1985, and december 31, 1988, harbored blastocystis. of these 46 children, 75% had exposure to well water or ...19938432086
[blastocystis hominis and abdominal pain in childhood].we report the clinical features observed in 10 children, ranging from 5 1/12 to 13 7/12 years of age, with intestinal infections caused by blastocystis hominis. a parasitological study of the stools was made by using the ethyl-acetate formol concentration technique and a count of the number of b. hominis per field was performed. in 8 of the cases, no other enteropathogens (viruses, bacteria or other parasites) were found, whereas in 2 cases giardia lamblia was also isolated. nine out of ten of t ...19938439071
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