Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| proteolytic processing of the proteins translated from the bottom component rna of cowpea mosaic virus. the primary and secondary cleavage reactions. | the products of translation of cowpea mosaic virus b-rna in rabbit reticulocyte lysates are proteolytically cleaved to form a specific set of proteins. the primary cleavage occurs as nascent peptide chains elongate to a size of about 150 kilodaltons yielding a 32k protein and, upon chain completion, a 170k protein. this cleavage reaction is inhibited by 3 mm iodoacetamide. the 170k protein, in turn, is cleaved to yield either of two pairs of proteins, a 110k and 60k pair or a 87k and 84k pair. t ... | 1984 | 6699013 |
| specificity of the actinomycin-d-sensitive function of some rna plant viruses. | the actinomycin-d(amd)-sensitive step of replication of three plant viruses - cowpea chlorotic mottle virus (ccmv), cowpea mosaic virus, and the cowpea strain of tobacco mosaic virus - in cowpea was specific for each virus. different combinations of the viruses were examined in which leaves were inoculated with one virus and incubated for 48 h until multiplication was almost resistant to amd. at that time, the same leaves were inoculated with a different virus and the ability of amd to inhibit m ... | 1984 | 6735660 |
| the location of the first aug codons in cowpea mosaic virus rnas. | we have made use of the known sequence of the 5' ends of both cpmv rnas to synthesise an oligodeoxynucleotide which can prime second-strand dna synthesis on full-length cdna copies of both rnas. by priming synthesis in the presence of dideoxynucleoside triphosphates, we have determined the positions of the first aug codons in each rna. these occur at positions 115 and 207 on m and b rna respectively. by using a cloned double-stranded dna fragment derived from near the 5' end of m rna as a primer ... | 1982 | 6897113 |
| comparison of in vivo and in vitro translation of cowpea mosaic virus rnas. | 1981 | 7236272 | |
| crystallographic studies of cowpea mosaic virus by electron microscopy and x-ray diffraction. | 1981 | 7241643 | |
| expression of an animal virus antigenic site on the surface of a plant virus particle. | to investigate if cowpea mosaic virus (cpmv) particles can be used to express foreign protein sequences, oligonucleotides encoding an epitope derived from vp1 of foot-and-mouth disease virus (fmdv) were cloned into the region of the cpmv genome encoding the small (s) coat protein. the chimeras were designed so that the foreign sequence was expressed either as an insertion or as a replacement for part of the wild-type sequence. while rna from both chimeras was able to replicate in cowpea protopla ... | 1993 | 7692669 |
| human immunodeficiency virus type 1-neutralizing antibodies raised to a glycoprotein 41 peptide expressed on the surface of a plant virus. | an oligonucleotide encoding the amino acids 731-752 of the gp41 envelope protein of the human immunodeficiency virus type 1 strain iiib, which is known to induce cross-reactive neutralizing antibodies in humans, was inserted into a full-length clone of the rna encoding the coat proteins of cowpea mosaic virus (rna 2 of cpmv). when transfected together with rna 1 of cpmv, transcribed rna 2 was able to replicate in plants and form infectious virions (cpmv-hiv). purified virions were injected subcu ... | 1995 | 7786579 |
| functional characterization of the proteolytic activity of the tomato black ring nepovirus rna-1-encoded polyprotein. | translation of tomato black ring virus (tbrv) rna-1 in a rabbit reticulocyte lysate leads to the synthesis of a 250k polyprotein which cleaves itself into smaller proteins of 50, 60, 120, and 190k. polypeptides synthesized from synthetic transcripts corresponding to different regions of tbrv rna-1 are processed only when they encode the 23k protein delimited earlier by sequence homology with the cowpea mosaic virus 24k protease. the proteolytic activity of this protein is completely lost by muta ... | 1995 | 7831791 |
| cold denaturation of an icosahedral virus. the role of entropy in virus assembly. | assembly of icosahedral viruses is not completely understood at the molecular level. the main puzzle is to answer how chemically identical protein subunits take up unique positionally dependent conformations during the process of assembly. the stability of the ribonucleoprotein particles of cowpea mosaic virus (cpmv) to pressures and subzero temperatures has been studied. at room temperature, reversible pressure denaturation of cpmv is obtained only in the presence of 5.0 m urea. on the other ha ... | 1995 | 7873549 |
| direct imaging of interactions between an icosahedral virus and conjugate f(ab) fragments by cryoelectron microscopy and x-ray crystallography. | the binding properties of seven mouse monoclonal antibodies (mcabs) raised against cowpea mosaic virus (cpmv) were characterized by conventional and inhibition enzyme-linked immunosorbent assay (elisa) technique. mcab binding to cpmv on electron microscope (em) grids was also assayed with gold-labeled anti-mouse antibodies. two of the seven mcabs (5b2 and 10b7) were found to bind tighter to cpmv than the others in the inhibition elisa and the em assay. f(ab) fragments from both of these mcabs we ... | 1994 | 7941346 |
| the ntp-binding motif in cowpea mosaic virus b polyprotein is essential for viral replication. | we have assessed the functional importance of the ntp-binding motif (ntbm) in the cowpea mosaic virus (cpmv) b-rna-encoded 58k domain by changing two conserved amino acids within the consensus a and b sites (gksrtgk500s and mdd545, respectively). both lys-500 to thr and asp-545 to pro substitutions are lethal as mutant b-rnas were no longer replicated in cowpea protoplasts. transiently produced mutant proteins were not able to support trans-replication of cpmv m-rna in cowpea protoplasts in cont ... | 1994 | 7964626 |
| capsid protein properties of cowpea aphid-borne mosaic virus and blackeye cowpea mosaic virus confirm the existence of two major subgroups of aphid-transmitted, legume-infecting potyviruses. | a study of the capsid proteins of different legume-infecting potyviruses using specific monoclonal antibodies on immunoblots of crude extracts from infected plants revealed that cowpea aphid-borne mosaic virus (camv) and blackeye cowpea mosaic virus (bicmv) have coat protein m(r) values of 32k and 35k, respectively. immunoblot comparisons of bicmv, peanut stripe mosaic virus (pstv), bean common mosaic virus (bcmv) and azuki bean mosaic virus (azmv) revealed equal reactivity of their 35k coat pro ... | 1994 | 7996147 |
| cowpea aphid borne mosaic virus-morocco and south african passiflora virus are strains of the same potyvirus. | high performance liquid chromatography (hplc) profiles of tryptic peptides and partial amino acid sequence analysis have been employed to establish the taxonomic status of the moroccan isolate of cowpea aphid-borne mosaic virus (cabmv). some previous reports have suggested cabmv to be very closely related to blackeye cowpea mosaic virus (b1cmv) while other reports have concluded that this relationship is distant. in this report a tryptic digest of the coat protein of cabmv-morocco was compared w ... | 1994 | 8002788 |
| development of cowpea mosaic virus as a high-yielding system for the presentation of foreign peptides. | it has recently been shown that cowpea plants can be infected with a cowpea mosaic virus (cpmv) chimera containing an antigenic site from foot-and-mouth disease virus (usha et al., virology 197, 366-374, 1993). analysis of progeny rna produced during such an infection has revealed that the inserted sequence is rapidly lost during serial passaging, probably by a process of homologous recombination. using the information gained from this analysis, we have redesigned the chimeras in such a way that ... | 1994 | 8030255 |
| differences in pressure stability of the three components of cowpea mosaic virus: implications for virus assembly and disassembly. | a comparison of pressure stability of empty capsids and ribonucleoprotein particles of cowpea mosaic virus (cpmv) is presented. a combination of high pressure and subdenaturing concentrations of urea was utilized to promote dissociation and denaturation. we found that rna plays an important role in stabilizing the particles as well as in conferring reversibility to the pressure-induced denaturation. dissociation and denaturation of the top component (empty capsid) was observed at 2.5 kbar and in ... | 1994 | 8031767 |
| replication and translation of cowpea mosaic virus rnas are tightly linked. | the genome of cowpea mosaic virus (cpmv) is divided among two positive strand rna molecules. b-rna is able to replicate independently from m-rna in cowpea protoplasts. replication of mutant b-transcripts could not be supported by co-inoculated wild-type b-rna, indicating that b-rna cannot be efficiently replicated in trans. hence replication of a b-rna molecule is tightly linked to its translation and/or at least one of the replicative proteins functions in cis only. remarkably also for efficien ... | 1994 | 8032269 |
| adaptation of positive-strand rna viruses to plants. | the vast majority of positive-strand rna viruses (more than 500 species) are adapted to infection of plant hosts. genome sequence comparisons of these plant rna viruses have revealed that most of them are genetically related to animal cell-infecting counterparts; this led to the concept of "superfamilies". comparison of genetic maps of representative plant and animal viruses belonging to the same superfamily (e.g. cowpea mosaic virus [cpmv] versus picornaviruses and tobacco mosaic virus versus a ... | 1994 | 8032285 |
| mutational analysis of aug codons of cowpea mosaic virus m rna. | the involvement of the aug codons at positions 115, 161, 512 and 524 in translation and infectivity of cowpea mosaic virus m rna was studied. mutations were introduced in each of these codons in a full length cdna clone of m rna and the effect of the mutations was examined by translation from in vitro transcripts of these mutant cdnas in rabbit reticulocyte lysates and by checking the replication of these transcripts in the presence of b rna in cowpea protoplasts and plants. it was found that au ... | 1993 | 8286445 |
| cis- and trans-acting elements in cowpea mosaic virus rna replication. | cowpea mosaic virus (cpmv) b-rna encodes the viral proteins required for viral rna replication while m-rna does so for the capsid proteins and functions required in cell-to-cell movement of the virus. accordingly, b-rna can replicate by itself, whereas m-rna can only replicate in the presence of b-rna. we have made heterologous sequence insertions at different positions in the open reading frame of b-rna, leaving the 5' and 3' non-coding ends intact. none of these mutant b-rnas were able to repl ... | 1993 | 8337819 |
| the involvement of cowpea mosaic virus m rna-encoded proteins in tubule formation. | on the surface of cowpea protoplasts inoculated with cowpea mosaic virus (cpmv), tubular structures containing virus particles have been found. such tubular structures are thought to be involved in cell-to-cell movement of cpmv in cowpea plants. to study the involvement of the 58k/48k and capsid proteins of cpmv in the formation of the tubular structures, mutations were introduced into m cdna clones from which infectious transcripts could be derived. no tubules were found on protoplasts inoculat ... | 1993 | 8345364 |
| defective cell-to-cell movement of cowpea mosaic virus mutant n123 is efficiently complemented by sunn-hemp mosaic virus. | during an infection with cowpea mosaic virus (cpmv) both virion assembly and formation of tubules associated with plasmodesmata are required for cell-to-cell movement. these functions are encoded by the m-rna of cpmv. to study the mechanism of cpmv movement, mutant n123 was used in complementation studies with sunn-hemp mosaic virus (shmv), a legume-infecting tobamovirus. previous studies have shown that n123 fails to spread in cowpea plants because of mutation(s) in its m-rna. however, the muta ... | 1993 | 8376964 |
| protoplasts transiently expressing the 200k coding sequence of cowpea mosaic virus b-rna support replication of m-rna. | in order to identify the viral polymerase involved in cowpea mosaic virus (cpmv) rna replication the 87k, 110k and 170k proteins as well as the complete 200k polyprotein of cpmv b-rna have been produced in cowpea protoplasts, using expression vectors based on the 35s promoter of cauliflower mosaic virus. cpmv-specific proteins were obtained that were indistinguishable from proteins found in cpmv-infected protoplasts. proteolytic processing of precursor proteins synthesized from the expression ve ... | 1993 | 8409945 |
| sequence data to settle the taxonomic position of bean common mosaic virus and blackeye cowpea mosaic virus isolates. | the nucleotide sequences of the coat protein genes and 3' non-translated regions (3'-ntrs) of three isolates of bean common mosaic virus (nl1, nl3 and ny15) and one isolate of blackeye cowpea mosaic virus (w) were determined. comparison of these sequences revealed that the coat proteins of nl1, ny15 and w were identical in size (287 amino acids) and exhibited an overall sequence similarity (94 to 97%), and 84 to 98% in their n-terminal regions. furthermore, their 3'-ntrs were very similar in len ... | 1993 | 8409946 |
| molecular cloning and sequence analysis of a segment from andean potato mottle virus b rna encoding the putative rna polymerase. | andean potato mottle virus (apmv), an endemic south american comovirus, has a bipartite genome consisting of two plus-strand rna molecules (m and b rna). we have cloned the 3' half of the b rna and identified the complete sequence of the putative apmv rna polymerase. the rna polymerase gene is part of a large polyprotein-encoding open reading frame. the putative, mature rna polymerase, as deduced by comparison with the related cowpea mosaic virus (cpmv), type member of the comovirus group, is 70 ... | 1993 | 8429307 |
| evidence that cowpea aphid-borne mosaic and blackeye cowpea mosaic viruses are two different potyviruses. | the immunoreactivity of a panel of polyclonal antibodies and monoclonal antibodies (mabs) raised against african isolates of potyviruses from cowpea and african yam bean was examined in elisas. a serological study including reference isolates followed by further characterization in differential hosts resulted in separation of the potyviruses into two distinct serogroups, one containing blackeye cowpea mosaic virus (blcmv) and the other containing cowpea aphid-borne mosaic virus (camv). using bio ... | 1993 | 8445363 |
| 3'-terminal nucleotide sequences important for the accumulation of cowpea mosaic virus m-rna. | the location of nucleotide sequences important in determining the extent of cowpea mosaic virus m-rna accumulation in cowpea protoplasts has been analyzed by deletion mutagenesis of full-length cdna clones from which infectious transcripts can be produced in vitro. the results suggest that cis-acting sequences which direct replication of m-rna by b-rna-encoded products are located within the 5'-terminal 524 nucleotides and the 3'-terminal 151 nucleotides. rna secondary structure predictions for ... | 1993 | 8460480 |
| cauliflower mosaic virus 35s promoter-controlled dna copies of cowpea mosaic virus rnas are infectious on plants. | clones have been constructed that contain full-length cdna copies of cowpea mosaic virus rna1 and rna2, downstream of the cauliflower mosaic virus 35s promoter. the clones, when linearized downstream of the viral sequences, give rise to cowpea mosaic virus-like symptoms when inoculated onto cowpea plants. viral rna and virions can be detected in the inoculated plants, demonstrating that the clones are directly infectious. | 1993 | 8492093 |
| the cowpea mosaic virus m rna-encoded 48-kilodalton protein is responsible for induction of tubular structures in protoplasts. | tubular structures extending from plasmodesmata in cowpea mosaic virus (cpmv)-infected tissue have been implicated to play an important role in cell-to-cell movement of this virus. using a cauliflower mosaic virus 35s promoter-based transient expression vector, we show that expression of only the cpmv m rna-encoded 48-kda protein (48k protein) in cowpea protoplasts is sufficient to induce these structures. strikingly, expression of the 48k protein in protoplasts from a number of nonhost plant sp ... | 1993 | 8497075 |
| the coat protein genes of squash mosaic virus: cloning, sequence analysis, and expression in tobacco protoplasts. | complementary dna of the middle-component rna of the melon strain of squash mosaic comovirus (sqmv) was cloned. clones containing the coat protein genes were identified by hybridization with a degenerate oligonucleotide synthesized according to the amino acid sequence of a purified peptide fragment of the sqmv large coat protein. a clone containing of 2.5 kbp cdna insert of sqmv m-rna was sequenced. the total insert sequence of 2510 bp included a 2373 bp open reading frame (orf) (encoding 791 am ... | 1993 | 8503782 |
| distinct functional domains in the cowpea mosaic virus movement protein. | cell-to-cell movement of cowpea mosaic virus particles in plants takes place with the help of tubules that penetrate presumably modified plasmodesmata. these tubules, which are built up by the virus-encoded 48-kda movement protein (mp), are also formed on single protoplast cells. to determine whether the mp contains different functional domains, the effect of mutations in its coding region was studied. mutations between amino acids 1 and 313 led to complete abolishment of the tubule-forming capa ... | 1996 | 8764083 |
| the 24 kda proteinases of comoviruses are virus-specific in cis as well as in trans. | to investigate the specificity of comoviral 24 kda ('24k') proteinases, a full-length cdna copy of red clover mottle virus (rcmv) rna 1 has been cloned downstream of a t7 promoter. translation in rabbit reticulocyte lysates of in vitro transcripts from this clone resulted in the synthesis of a 200k protein which was processed in a manner similar to that of the equivalent protein from cowpea mosaic virus (cpmv). full-length cdna clones of the rna 1 molecules of rcmv and cpmv were used to create h ... | 1996 | 8811039 |
| stimulation of neutralizing antibodies to human immunodeficiency virus type 1 in three strains of mice immunized with a 22 amino acid peptide of gp41 expressed on the surface of a plant virus. | a plant virus, cowpea mosaic virus, expressing a 22 amino acid peptide 731-752 of the gp41 glycoprotein of human immunodeficency virus type 1 (hiv-1 iiib), was shown previously to stimulate hiv-1 cross reactive neutralizing antibodies in adult c57/bl6 mice. here some parameters concerning the stimulation of hiv-1-specific neutralizing and elisa antibody have been determined in adult c57/bl6, c3h/he-mg and balb/c mice. two injections per mouse of all cpmv-hiv/1 doses tested (100, 10 and 1 microgr ... | 1996 | 8817828 |
| potential of chimaeric plant virus particles as novel, stable vaccines. | technology has been developed for the expression of multiple copies of epitopes from human and animal pathogens on the surface of assembled particles of a plant virus (cowpea mosaic virus). the technology, termed the chimaeric virus particle (cvp) technology, can be exploited for the production of vaccines in plants. each chimaeric virus particle contains 60 copies of the foreign peptide which are expressed in highly exposed positions on the surface of the virus particle. viral and bacterial epi ... | 1996 | 8854018 |
| capsid proteins of cowpea mosaic virus transiently expressed in protoplasts form virus-like particles. | the coding regions for cowpea mosaic virus (cpmv) capsid proteins vp37 and vp23 were introduced separately into a transient plant expression vector containing an enhanced camv 358 promoter. significant expression of either capsid protein was observed only in protoplasts transfected simultaneously with both constructs. immunosorbent electron microscopy revealed the presence of virus-like particles in extracts of these protoplasts. an extract of protoplasts transfected with both constructs togethe ... | 1996 | 8862434 |
| the movement proteins of cowpea mosaic virus and cauliflower mosaic virus induce tubular structures in plant and insect cells. | the movement proteins (mp) of cowpea mosaic virus and cauliflower mosaic virus (camv) are associated with tubular structures in vivo which participate in the transmission of virus particles from cell to cell. both proteins have been expressed in plant protoplasts and insect cells. in all cases, immunofluorescent histochemistry showed that the mps accumulate intracellularly as tubular extensions projecting from the cell surface. additionally, electron microscopy revealed intracellular mp aggregat ... | 1996 | 8922481 |
| the development of cowpea mosaic virus as a potential source of novel vaccines. | epitopes from human rhinovirus 14 (hrv-14) and human immunodeficiency virus type (hiv-1) have been expressed on the surface of particles of the plant virus, cowpea mosaic virus (cpmv). the chimaeras retain their ability to grow in plants and large quantities of virions can be easily purified. immunological studies have shown that purified particles have the antigenic properties of the insert, and, in the case of the hiv-1 chimaera, can elicit the production of neutralising antibodies in mice. th ... | 1996 | 8957673 |
| the cowpea mosaic virus rna 1-encoded 112 kda protein may function as a vpg precursor in vivo. | processing of the 112 kda ('112k') protein encoded by cowpea mosaic virus rna 1 was examined in cowpea mesophyll protoplasts using a transient expression system. cleavage of the 112k protein occurred via two alternative pathways either into vpg and 110k (24k + 87k) or into 26k (vpg + 24k) and 87k proteins. the 26k protein can be further cleaved into vpg and 24k proteins. the results support a model in which the 112k protein functions as the precursor of vpg during initiation of replication. | 1995 | 9049386 |
| plant-derived vaccine protects target animals against a viral disease. | the successful expression of animal or human virus epitopes on the surface of plant viruses has recently been demonstrated. these chimeric virus particles (cvps) could represent a cost-effective and safe alternative to conventional animal cell-based vaccines. we report the insertion of oligonucleotides coding for a short linear epitope from the vp2 capsid protein of mink enteritis virus (mev) into an infectious cdna clone of cowpea mosaic virus and the successful expression of the epitope on the ... | 1997 | 9062924 |
| structure-based design of peptide presentation on a viral surface: the crystal structure of a plant/animal virus chimera at 2.8 a resolution. | we employed a genetically engineered icosahedral plant virus, cowpea mosaic virus (cpmv), as an expression and presentation system to display a 14 amino acid linear antigenic epitope found in a capsid protein of human rhinovirus 14 (hrv14). | 1996 | 9079380 |
| isolation and characterization of tubular structures of cowpea mosaic virus. | tubular structures involved in the cell-to-cell movement of cowpea mosaic virus (cpmv) were partially purified from infected cowpea protoplasts to identify the structural components. a relatively pure fraction could be obtained by differential centrifugation and this was analysed by page and immunoblotting. besides the movement protein (mp) and capsid proteins (cp) of cpmv, no other major infection-specific proteins could be detected, suggesting that host proteins are not a major structural comp ... | 1997 | 9400966 |
| studies on the movement of cowpea mosaic virus using the jellyfish green fluorescent protein. | the jellyfish green fluorescent protein (gfp) coding sequence was used to replace the coat protein (cp) genes in a full-length cdna clone of cpmv rna-2. transcripts of this construct were replicated in the presence of rna-1 in cowpea protoplasts, and gfp expression could be readily detected by fluorescent microscopy. it was not possible to infect cowpea plants with these transcripts, but combined with a mutant rna-2, in which the 48-kda movement protein (mp) gene has been deleted infection did o ... | 1998 | 9501035 |
| sequences of the coat protein gene of five peanut stripe virus (pstv) strains from thailand and their evolutionary relationship with other bean common mosaic virus sequences. | the coat protein gene and part of the 3' non-coding region of five strains of peanut stripe virus (pstv) from thailand have been cloned and sequenced. phylogenetic comparisons of these strains, known as t1, t3, t5, t6 and t7, and related sequences showed that these strains are indeed strains of pstv. further, pstv strains appear to be related to each other according to their geographic origin. that is, the thai strains are more closely related to each other than they are to strains from the usa ... | 1998 | 9787652 |
| the neutralizing antibody response against a conserved region of human immunodeficiency virus type 1 gp41 (amino acid residues 731-752) is uniquely directed against a conformational epitope. | amino acids 731-752 (731prgpdrpegieeeggerdrdrs752) of the transmembrane glycoprotein gp41 of human immunodeficiency virus type 1 (hiv-1) are conserved in most virus isolates and are controversially reported to be implicated in virus neutralization. the humoral response in infected patients against this region is poor and humans immunized with gp160 show high levels of antibodies against the peptide but poor neutralization titres. nonetheless, several groups have succeeded in obtaining neutralizi ... | 1998 | 9820146 |
| intranasal immunization with a plant virus expressing a peptide from hiv-1 gp41 stimulates better mucosal and systemic hiv-1-specific iga and igg than oral immunization. | control of pandemic human immunodeficiency virus type 1 (hiv-1) infection ideally requires specific mucosal immunity to protect the genital regions through which transmission more often occurs. thus a vaccine that stimulates a disseminated mucosal and systemic protective immune response would be extremely useful. here we have investigated the ability of a chimeric plant virus, cowpea mosaic virus (cpmv), expressing a 22 amino acid peptide (residues 731-752) of the transmembrane gp41 protein of h ... | 1998 | 9839930 |
| chimeric plant virus particles administered nasally or orally induce systemic and mucosal immune responses in mice. | the humoral immune responses to the d2 peptide of fibronectin-binding protein b (fnbp) of staphylococcus aureus, expressed on the plant virus cowpea mosaic virus (cpmv), were evaluated after mucosal delivery to mice. intranasal immunization of these chimeric virus particles (cvps), either alone or in the presence of iscom matrix, primed cpmv-specific t cells and generated high titers of cpmv- and fnbp-specific immunoglobulin g (igg) in sera. furthermore, cpmv- and fnbp-specific iga and igg could ... | 1999 | 9882293 |
| the cleavable carboxyl-terminus of the small coat protein of cowpea mosaic virus is involved in rna encapsidation. | the site of cleavage of the small coat protein of cowpea mosaic virus has been precisely mapped and the proteolysis has been shown to result in the loss of 24 amino acids from the carboxyl-terminus of the protein. a series of premature termination and deletion mutants was constructed to investigate the role or roles of these carboxyl-terminal amino acids in the viral replication cycle. mutants containing premature termination codons at or downstream of the cleavage site were viable but reverted ... | 1999 | 10049828 |
| analysis of the ability of five adjuvants to enhance immune responses to a chimeric plant virus displaying an hiv-1 peptide. | the ability of five different adjuvants (alum, complete freund's adjuvant, quil a, adjuprime and ribi) to stimulate humoral and t-cell mediated immune responses against a purified chimeric virus particle was investigated. each adjuvant was administered subcutaneously to adult mice together with 10 microg of wildtype (wt) cowpea mosaic virus (cpmv) or a chimeric cpmv displaying the hiv-1 gp41 peptide, residues 731-752. all preparations elicited strong antibody responses to cpmv, but quil a elicit ... | 1999 | 10195771 |
| pseudomonas aeruginosa outer-membrane protein f epitopes are highly immunogenic in mice when expressed on a plant virus. | a synthetic peptide (peptide 10) representing a surface-exposed, linear b cell epitope from outer-membrane (om) protein f of pseudomonas aeruginosa was shown previously to afford protection in mice from p. aeruginosa infection. this peptide was expressed in tandem with the protein f peptide 18 on each of the two coat proteins of cowpea mosaic virus (cpmv). the chimaeric virus particles (cvps) expressing the peptides on the s (small) coat protein (cpmv-pae4) and l (large) coat protein (cpmv-pae5) ... | 1999 | 10206701 |
| immunogenicity of peptides derived from a fibronectin-binding protein of s. aureus expressed on two different plant viruses. | the d2 peptide derived from an s. aureus fibronectin-binding protein (fnbp) was expressed on the surface of the icosahedral cowpea mosaic virus (amino acids 1-30 of d2) or on the rod-shaped potato virus x (amino acids 1-38 of d2), termed cpmv-mast1 and pvx-mast8, respectively. mice and rats were immunized subcutaneously with cpmv-mast1 and mice with pvx-mast8 in adjuvant and high titres of fnbp-specific antibody were obtained. the mouse igg was predominantly of the igg2a and igg2b isotypes, whic ... | 1999 | 10217582 |
| position-dependent processing of peptides presented on the surface of cowpea mosaic virus. | the plant virus cowpea mosaic virus (cpmv) has been developed as an epitope-presentation system. numerous epitopes have been expressed in the betab-betac loop of the cpmv small coat protein, all of which undergo a cleavage reaction between their two carboxy-terminal residues. although many peptides presented in this manner give an authentic immune response, this was not the case for the nim-1a epitope from human rhinovirus-14. crystallography revealed significant differences between the structur ... | 1999 | 10223342 |
| cowpea mosaic virus-based vaccines. | 1999 | 10394721 | |
| scope for using plant viruses to present epitopes from animal pathogens. | epitope presentation to the immune system for vaccination purposes can be achieved either via an inactivated or attenuated form of a pathogen or via its isolated antigenic sequences. when free, these peptides can adopt a variety of conformations, most of which will not exist in their native environment. conjugation to carrier proteins restricts mobility of the peptides and increases their immunogenicity. a high local concentration of epitopes boosts the immune response further and can be generat ... | 1998 | 10398492 |
| complete nucleotide sequence of bean pod mottle virus rna1: sequence comparisons and evolutionary relationships to other comoviruses. | the complete nucleotide sequence of bean pod mottle comovirus (bpmv) rna 1 was determined. it is 5983 nucleotides long, excluding the poly(a) tail, and encodes a polyprotein of 1850 amino acid (aa) residues. multiple alignments of the deduced aa sequence of bpmv polyprotein with those of cowpea mosaic virus (cpmv), red clover mottle virus (rcmv) and cowpea severe mosaic virus (cpsmv) indicated that bpmv rna1 encodes the predicted set of five mature proteins: the equivalent of cpmv 32k protease c ... | 1999 | 10403699 |
| a chimaeric plant virus vaccine protects mice against a bacterial infection. | the plant virus cowpea mosaic virus (cpmv) is an efficient carrier of foreign peptides for the generation of strong humoral immune responses. peptides derived from both viruses and bacteria are strongly immunogenic when displayed on the surface of cpmv and elicit high titres of peptide-specific antibody. however, the protective effects of antibodies generated using bacterial epitopes in this system have yet to be demonstrated. in this study the ability of chimaeric virus particles (cvps) to affo ... | 1999 | 10463172 |
| studies on hybrid comoviruses reveal the importance of three-dimensional structure for processing of the viral coat proteins and show that the specificity of cleavage is greater in trans than in cis. | a series of cowpea mosaic virus (cpmv)-based hybrid comoviral rna-2 molecules have been constructed. in these, the region encoding both the large (l) and small (s) viral coat proteins was replaced by the equivalent region from bean pod mottle virus (bpmv). the hybrid rna-2 molecules were able to replicate in cowpea protoplasts in the presence of cpmv rna-1. though processing of the hybrid polyproteins by the cpmv-specific 24k proteinase at the site between the 58/48k and l proteins could readily ... | 1999 | 10544093 |
| structural fingerprinting: subgrouping of comoviruses by structural studies of red clover mottle virus to 2.4-a resolution and comparisons with other comoviruses. | red clover mottle virus (rcmv) is a member of the comoviruses, a group of picornavirus-like plant viruses. the x-ray structure of rcmv strain s has been determined and refined to 2.4 a. the overall structure of rcmv is similar to that of two other comoviruses, cowpea mosaic virus (cpmv) and bean pod mottle virus (bpmv). the sequence of the coat proteins of rcmv strain o were modeled into the capsid structure of strain s without causing any distortion, confirming the close resemblance between the ... | 2000 | 10590139 |
| the refined crystal structure of cowpea mosaic virus at 2.8 a resolution. | comoviruses are a group of plant viruses in the picornavirus superfamily. the type member of comoviruses, cowpea mosaic virus (cpmv), was crystallized in the cubic space group i23, a = 317 a and the hexagonal space group p6(1)22, a = 451 a, c = 1038 a. structures of three closely similar nucleoprotein particles were determined in the cubic form. the roughly 300-a capsid was similar to the picornavirus capsid displaying a pseudo t = 3 (p = 3) surface lattice. the three beta-sandwich domains adopt ... | 1999 | 10603314 |
| immunogenic and antigenic dominance of a nonneutralizing epitope over a highly conserved neutralizing epitope in the gp41 envelope glycoprotein of human immunodeficiency virus type 1: its deletion leads to a strong neutralizing response. | the kennedy peptide, (731)prgpdrpegieeeggerdrdrs(752), from the cytoplasmic domain of the gp41 transmembrane envelope glycoprotein of hiv-1 contains a conformationally dependent neutralizing epitope (erdrd) and a linear nonneutralizing epitope (ieee). no recognized murine t cell epitope is present. the peptide usually stimulates virus-specific antibody, but this is not always neutralizing. here we show that ieee (or possibly ieee plus adjacent sequence) is immunogenically and antigenically domin ... | 2000 | 10612661 |
| complementation of the host range restriction of southern cowpea mosaic virus in bean by southern bean mosaic virus. | vigna unguiculata (cowpea) and phaseolus vulgaris (common bean) are permissive hosts for southern cowpea mosaic virus (scpmv) and southern bean mosaic virus (sbmv), respectively. neither of these two sobemoviruses systemically infects the permissive host of the other. although bean cells are permissive for scpmv rna synthesis, they do not support the assembly of this virus. thus, the host range restriction of scpmv in bean may occur at the level of movement and may involve the inability of scpmv ... | 2000 | 10612668 |
| evidence for participation of rna 1-encoded elicitor in cowpea mosaic virus-mediated concurrent protection. | the cowpea (vigna unguiculata) line arlington, inoculated with cowpea mosaic virus (cpmv), showed no symptoms, and no infectivity or accumulation of capsid antigen was detected at several days after inoculation. coinoculation, but not sequential inoculation, of cpmv with similar concentrations of another comovirus; cowpea severe mosaic virus (cpsmv), resulted in reduced numbers of cpsmv-induced lesions. this apparent, cpmv-mediated reduction in number of cpsmv-induced infection centers was terme ... | 2000 | 10639316 |
| engineering cowpea mosaic virus rna-2 into a vector to express heterologous proteins in plants. | a series of new cowpea mosaic virus (cpmv) rna-2-based expression vectors were designed. the jellyfish green fluorescent protein (gfp) was introduced between the movement protein (mp) and the large (l) coat protein or downstream of the small (s) coat protein. release of the gfp inserted between the mp and l proteins was achieved by creating artificial processing sites each side of the insert, either by duplicating the mp-l cleavage site or by introducing a sequence encoding the foot-and-mouth di ... | 2000 | 10662612 |
| mutational analysis of the cowpea mosaic virus movement protein. | cowpea mosaic virus moves from cell-to-cell in a virion form through tubular structures that are assembled in modified plasmodesmata. similar tubular structures are formed on the surface of protoplasts inoculated with cowpea mosaic virus. the rna 2-encoded movement protein (mp) is responsible for the induction and formation of these structures. to define functional domains of the mp, an alanine-substitution mutagenesis was performed on eight positions in the mp, including two conserved sequence ... | 2000 | 10662615 |
| glycosylation of the capsid proteins of cowpea mosaic virus: a reinvestigation shows the absence of sugar residues. | the previously reported (partridge et al., nature 247, 391-392, 1974 ) glycosylation of the capsid proteins of cowpea mosaic virus (cpmv) has been reinvestigated. in initial studies, a preparation of purified cpmv particles was hydrolysed with hcl and amino acids and sugars were derivatized with o-phthalaldehyde (opa). no glucosamine or galactosamine, amino sugars previously reported to occur in significant quantities in cpmv capsids, could be detected by reverse-phase high-performance liquid ch ... | 2000 | 10725439 |
| chimeric animal and plant viruses expressing epitopes of outer membrane protein f as a combined vaccine against pseudomonas aeruginosa lung infection. | outer membrane protein f of pseudomonas aeruginosa has vaccine efficacy against infection by p. aeruginosa as demonstrated in a variety of animal models. through the use of synthetic peptides, three surface-exposed epitopes have been identified. these are called peptides 9 (aa 261-274 in the mature f protein, tdaynqklserran), 10 (aa 305-318, nataegrainrrve), and 18 (aa 282-295, neygveggrvnavg). both the peptide 9 and 10 epitopes are protective when administered as a vaccine. in order to develop ... | 2000 | 10727884 |
| influence of three-dimensional structure on the immunogenicity of a peptide expressed on the surface of a plant virus. | the influence of peptide structure on immunogenicity has been investigated by constructing a series of cowpea mosaic virus (cpmv) chimaeras expressing the 14 amino acid nim-1a epitope from human rhinovirus 14 (hrv-14) at different positions on the capsid surface. biochemical and crystallographic analysis of a cpmv/hrv chimaera expressing the nim-1a epitope inserted into the betac'-betac" loop of the s protein revealed that, although the inserted peptide was free at its c-terminus, it adopted a c ... | 2000 | 10822251 |
| cowpea mosaic virus infection induces a massive proliferation of endoplasmic reticulum but not golgi membranes and is dependent on de novo membrane synthesis. | replication of cowpea mosaic virus (cpmv) is associated with small membranous vesicles that are induced upon infection. the effect of cpmv replication on the morphology and distribution of the endomembrane system in living plant cells was studied by expressing green fluorescent protein (gfp) targeted to the endoplasmic reticulum (er) and the golgi membranes. cpmv infection was found to induce an extensive proliferation of the er, whereas the distribution and morphology of the golgi stacks remain ... | 2000 | 10864669 |
| co-expression of the capsid proteins of cowpea mosaic virus in insect cells leads to the formation of virus-like particles. | the regions of rna-2 of cowpea mosaic virus (cpmv) that encode the large (l) and small (s) coat proteins were expressed either individually or together in spodoptera frugiperda (sf21) cells using baculovirus vectors. co-expression of the two coat proteins from separate promoters in the same construct resulted in the formation of virus-like particles whose morphology closely resembled that of native cpmv virions. no such particles were formed when the individual l and s proteins were expressed. s ... | 2000 | 11086140 |
| cowpea mosaic virus as a vaccine carrier of heterologous antigens. | the plant virus, cowpea mosaic virus (cpmv), has been developed as an expression and presentation system to display antigenic epitopes derived from a number of vaccine targets including infectious disease agents and tumors. these chimeric virus particles (cvps) could represent a cost-effective and safe alternative to live replicating virus and bacterial vaccines. a number of cvps have now been generated and their immunogenicity examined in a number of animal species. this review details the humo ... | 2001 | 11280928 |
| complete nucleotide sequence of sesbania mosaic virus: a new virus species of the genus sobemovirus. | the complete nucleotide sequence of the sesbania mosaic virus (semv) genomic rna was determined by sequencing overlapping cdna clones. the semv genome is 4149 nucleotides in length and encodes four potential overlapping open reading frames (orfs). comparison of the nucleotide sequence and the deduced amino acid sequence of the four orfs of semv with that of other sobemoviruses revealed that semv was closest to southern bean mosaic virus arkansas isolate (sbmv-ark, 73% identity). the 5' non-codin ... | 2001 | 11315633 |
| an ab initio algorithm for low-resolution 3-d reconstructions from cryoelectron microscopy images. | a statistical method for determining low-resolution 3-d reconstructions of virus particles from cryoelectron microscope images by an ab initio algorithm is described. the method begins with a novel linear reconstruction method that generates a spherically symmetric reconstruction, which is followed by a nonlinear reconstruction method implementing an expectation-maximization procedure using the spherically symmetric reconstruction as an initial condition and resulting in a reconstruction with ic ... | 2006 | 11472085 |
| in vitro analysis of an rna binding site within the n-terminal 30 amino acids of the southern cowpea mosaic virus coat protein. | southern cowpea mosaic virus (scpmv) is a positive-sense rna virus with t = 3 icosahedral symmetry. the coat protein (cp) has two domains, the random (r) domain and the shell (s) domain. the r domain is formed by the n-terminal 64 amino acids (aa) and is localized to the interior of the particle where it is expected to interact with the viral rna. the r domain (aa 1--57) was expressed in escherichia coli as a recombinant protein (rwtr) containing a nonviral c-terminal extension with two histidin ... | 2001 | 11485399 |
| disruption of leaves and initial extraction of wildtype cpmv virus as a basis for producing vaccines from plants. | wildtype cowpea mosaic virus (cpmv) was extracted from fresh and frozen plant material by methods suitable for large-scale application. deep freezing, crushing, and thawing in water or buffers gave 0.6+/-0.2 mg g(-1) of virus after up to 24 h. release from sliced fresh leaves was lower at 0.14+/-0.03 mg g(-1). homogenisation of frozen leaves for 1 min increased yield to a maximum, on average of 3.5 mg g(-1) but varying between batches from 2.2 to 4.8 mg g(-1) virus long term storage at -80 degre ... | 2002 | 11689247 |
| membrane activity of the southern cowpea mosaic virus coat protein: the role of basic amino acids, helix-forming potential, and lipid composition. | southern cowpea mosaic virus (scpmv) is a spherical rna virus with t = 3 icosahedral symmetry. the particle is composed of 180 subunits of the coat protein (cp) and one copy of the positive-sense viral rna. the cp has two domains, the random (r) domain formed by the n-terminal 64 aa and the shell (s) domain (aa 65--260). the r domain is highly charged, with 11 of the n-terminal 30 residues being basic. it is localized to the interior of the native particle where it may interact with the viral rn ... | 2001 | 11878899 |
| mutational analysis of the genome-linked protein of cowpea mosaic virus. | in this study we have performed a mutational analysis of the cowpea mosaic comovirus (cpmv) genome-linked protein vpg to discern the structural requirements necessary for proper functioning of vpg. either changing the serine residue linking vpg to rna at a tyrosine or a threonine or changing the position of the serine from the n-terminal end to position 2 or 3 abolished virus infectivity. some of the mutations affected the cleavage between the vpg and the 58k atp-binding protein in vitro, which ... | 2001 | 11883002 |
| characterization of plant proteins that interact with cowpea mosaic virus '60k' protein in the yeast two-hybrid system. | cowpea mosaic virus (cpmv) replication occurs in close association with small membranous vesicles in the host cell. the cpmv rna1-encoded 60 kda nucleotide-binding protein ('60k') plays a role in the formation of these vesicles. in this study, five cellular proteins were identified that interacted with different domains of 60k using a yeast two-hybrid search of an arabidopsis cdna library. two of these host proteins (termed vap27-1 and vap27-2), with high homology to the vap33 family of snare-li ... | 2002 | 11907339 |
| novel strategy for inhibiting viral entry by use of a cellular receptor-plant virus chimera. | the plant virus cowpea mosaic virus (cpmv) has recently been developed as a biomolecular platform to display heterologous peptide sequences. such cpmv-peptide chimeras can be easily and inexpensively produced in large quantities from experimentally infected plants. this study utilized the cpmv chimera platform to create an antiviral against measles virus (mv) by displaying a peptide known to inhibit mv infection. this peptide sequence corresponds to a portion of the mv binding site on the human ... | 2002 | 11932408 |
| coalescence of the sites of cowpea mosaic virus rna replication into a cytopathic structure. | cowpea mosaic virus (cpmv) replication induces an extensive proliferation of endoplasmic reticulum (er) membranes, leading to the formation of small membranous vesicles where viral rna replication takes place. using fluorescent in situ hybridization, we found that early in the infection of cowpea protoplasts, cpmv plus-strand rna accumulates at numerous distinct subcellular sites distributed randomly throughout the cytoplasm which rapidly coalesce into a large body located in the center of the c ... | 2002 | 12021357 |
| cowpea mosaic virus 32- and 60-kilodalton replication proteins target and change the morphology of endoplasmic reticulum membranes. | cowpea mosaic virus (cpmv) replicates in close association with small membranous vesicles that are formed by rearrangements of intracellular membranes. to determine which of the viral proteins are responsible for the rearrangements of membranes and the attachment of the replication complex, we have expressed individual cpmv proteins encoded by rna1 in cowpea protoplasts by transient expression and in nicotiana benthamiana plants by using the tobacco rattle virus (trv) expression vector. the 32-k ... | 2002 | 12021362 |
| phloem loading and unloading of cowpea mosaic virus in vigna unguiculata. | within their host plants, viruses spread from the initially infected cell through plasmodesmata to neighbouring cells (cell-to-cell movement), until reaching the phloem for rapid invasion of the younger plant parts (long-distance or vascular movement). cowpea mosaic virus (cpmv) moves from cell-to-cell as mature virions via tubules constructed of the viral movement protein (mp). the mechanism of vascular movement, however, is not well understood. the characteristics of vascular movement of cpmv ... | 2002 | 12029165 |
| complete nucleotide sequence and possible genomic expression strategy of a chinese isolate of broad bean wilt virus. | the complete nucleotide sequence of isolate b935 of the rnas of broad bean wilt virus2 (bbwv2) was determined. rna1 contained 5956 nucleotide (nt) residues in length exclusive the 3' terminal poly(a)tail, and encoded a single long open reading frame (orf) with a molecular weight of 210 063 (210 kd). rna2 was composed of 3 601 nt residues exclusive of the poly(a) at the 3' end, and encoded a single long orf with a molecular weight of 119 002 (119 kd). the determination of the n terminal sequences ... | 2001 | 12053188 |
| the cytoskeleton and the secretory pathway are not involved in targeting the cowpea mosaic virus movement protein to the cell periphery. | the movement protein (mp) of cowpea mosaic virus (cpmv) forms tubules on infected protoplasts and through plasmodesmata in infected plants. in protoplasts the mp fused to gfp (mp-gfp) was shown to localize in peripheral punctate structures and in long tubular structures extending from the protoplast surface. using cytoskeletal assembly inhibitors (latrunculin b and oryzalin) and an inhibitor of the secretory pathway (brefeldin a), targeting of the mp to the peripheral punctate structures was dem ... | 2002 | 12083835 |
| natural supramolecular building blocks. wild-type cowpea mosaic virus. | cowpea mosaic virus (cpmv) can be isolated in gram quantities, possesses a structure that is known to atomic resolution, and is quite stable. it is therefore of potential use as a molecular entity in synthesis, particularly as a building block on the nanochemical scale. cpmv was found to possess a lysine residue with enhanced reactivity in each asymmetric unit, and thus 60 such lysines per virus particle. the identity of this residue was established by a combination of acylation, protein digesti ... | 2002 | 12144924 |
| natural supramolecular building blocks. cysteine-added mutants of cowpea mosaic virus. | wild-type cowpea mosaic virus (cpmv) displays no cysteine side chains on the exterior capsid surface and is therefore relatively unreactive with thiol-selective reagents. four cpmv mutants bearing cysteine residues in one of two exterior positions of the asymmetric unit were created. the mutants were shown to aggregate by virtue of disulfide bond formation in the absence of added reducing agent, bind to metallic gold, and undergo selective reactions at the introduced thiol residues. controlled a ... | 2002 | 12144925 |
| transgenic plants expressing hc-pro show enhanced virus sensitivity while silencing of the transgene results in resistance. | nicotiana benthamiana plants were engineered to express sequences of the helper component-proteinase (hc-pro) of cowpea aphid-borne mosaic potyvirus (cabmv). the sensitivity of the transgenic plants to infection with parental and heterologous viruses was studied. the lines expressing hc-pro showed enhanced symptoms after infection with the parental cabmv isolate and also after infection with a heterologous potyvirus, potato virus y (pvy) and a comovirus, cowpea mosaic virus (cpmv). on the other ... | 2002 | 12206307 |
| molecular structures of viruses from raman optical activity. | a vibrational raman optical activity (roa) study of a range of different structural types of virus exemplified by filamentous bacteriophage fd, tobacco mosaic virus, satellite tobacco mosaic virus, bacteriophage ms2 and cowpea mosaic virus has revealed that, on account of its sensitivity to chirality, roa is an incisive probe of their aqueous solution structures at the molecular level. protein roa bands are especially prominent from which, as we have shown by comparison with the roa spectra of p ... | 2002 | 12237443 |
| rna-mediated virus resistance: role of repeated transgenes and delineation of targeted regions. | resistance to cowpea mosaic virus (cpmv) in transgenic nicotiana benthamiana plants is rna mediated. in resistant cpmv movement protein (mp) gene-transformed lines, transgene steady state mrna levels were low, whereas nuclear transcription rates were high, implying that a post-transcriptional gene-silencing mechanism is at the base of the resistance. the silencing mechanism can also affect potato virus x (pvx) rnas when they contain cpmv mp gene sequences. in particular, sequences situated in th ... | 1996 | 12239378 |
| agroinfection as a rapid method for propagating cowpea mosaic virus-based constructs. | to increase the efficiency of infections with cowpea mosaic virus (cpmv)-based constructs, clones suitable for agroinfection were constructed. full-length copies of rna-1 and rna-2 were inserted between the sequence of a cauliflower mosaic virus (camv) 35s promoter and a nos terminator and were introduced into the agrobacterium tumefaciens plasmid, pbinplus. infiltration of leaves of either nicotiana benthamiana or cowpea (vigna unguiculata) with a bacterial suspension containing a mixture of th ... | 2002 | 12270666 |
| protein-rna interactions and virus stability as probed by the dynamics of tryptophan side chains. | the correlation between dynamics and stability of icosahedral viruses was studied by steady-state and time-resolved fluorescence approaches. we compared the environment and dynamics of tryptophan side chains of empty capsids and ribonucleoprotein particles of two icosahedral viruses from the comovirus group: cowpea mosaic virus (cpmv) and bean pod mottle virus (bpmv). we found a great difference between tryptophan fluorescence emission spectra of the ribonucleoprotein particles and the empty cap ... | 2002 | 12359712 |
| opening the high-pressure domain beyond 2 kbar to protein and virus crystallography--technical advance. | the combined use of a diamond anvil cell and ultrashort-wavelength undulator radiation has allowed the collection of high-resolution diffraction data from protein and virus crystals submitted to hydrostatic pressures beyond 2 kbar. crystals of cubic cowpea mosaic virus (cpmv) can be compressed to at least 3.5 kbar. diffraction from cpmv crystals displaying an unusual disorder at atmospheric pressure was considerably enhanced by application of pressure. these experiments suggest that pressure may ... | 2002 | 12377126 |
| subcellular location of the helper component-proteinase of cowpea aphid-borne mosaic virus. | the helper component-proteinase (hc-pro) of cowpea aphid-borne mosaic virus (cabmv) was expressed in escherichia coli and used to obtain hc-pro antiserum that was used as an analytical tool for hc-pro studies. the antiserum was used in immunofluorescence assays to study the subcellular location of hc-pro expressed with other viral proteins in cowpea protoplasts in a natural cabmv infection, or in protoplasts transfected with a transient expression construct expressing hc-pro separately from othe ... | 2002 | 12416684 |
| blue fluorescent antibodies as reporters of steric accessibility in virus conjugates. | nonenveloped viruses provide the chemist with large, preassembled polyvalent protein scaffolds for modification. these structures are typically porous to small molecules but not to large ones. the solution-phase structures and reactivities of such assemblies may be substantially different than indicated by x-ray crystal structures. here, the attachment of organic compounds to either the inside or outside surface of the cowpea mosaic virus (cpmv) coat protein was verified with an indicating antib ... | 2015 | 12526690 |
| studies on the c-terminus of the cowpea mosaic virus movement protein. | cowpea mosaic virus (cpmv) spreads from cell-to-cell as virus particles through tubular structures in modified plasmodesmata which are composed of viral movement protein (mp). mutational analysis of the mp has revealed that the n-terminal and central regions of the mp are involved in tubule formation and that the c-terminal domain probably has a role in the interactions with virus particles. by constructing c-terminal deletion mutants and comoviral hybrid mps, it was possible to delineate the c- ... | 2003 | 12556992 |
| cowpea mosaic virus: from the presentation of antigenic peptides to the display of active biomaterials. | the potential of cowpea mosaic virus (cpmv), a plant icosahedral virus, for the presentation of foreign peptides and proteins is reported. the most prominent feature at the virus surface is a region of the smaller of the two coat proteins (s) which has been extensively used for the insertion of foreign peptides. given the availability of the three-dimensional structure of the native virus and the amenability of foreign peptide-expressing cpmv chimeras to crystallisation, immunological data can b ... | 2002 | 12602357 |
| hybrid virus-polymer materials. 1. synthesis and properties of peg-decorated cowpea mosaic virus. | cowpea mosaic virus was derivatized with poly(ethylene glycol) to give well-controlled loadings of polymer on the outer surface of the coat protein assembly. the resulting conjugates displayed altered densities and immunogenicities, consistent with the known chemical and biological properties of peg. these studies make cpmv potentially useful as a tailored vehicle for drug delivery. | 2015 | 12741758 |
| effect of priming/booster immunisation protocols on immune response to canine parvovirus peptide induced by vaccination with a chimaeric plant virus construct. | expression of a 17-mer peptide sequence from canine parvovirus expressed on cowpea mosaic virus (cpmv) to form chimaeric virus particles (cvps) creates vaccine antigens that elicit strong anti-peptide immune responses in mice. systemic (subcutaneous, s.c.) immunisation and boosting with such cvp constructs produces igg(2a) serum antibody responses, while mucosal (intranasal, i.n.) immunisation and boosting elicits intestinal iga responses. combinations of systemic and mucosal routes for priming ... | 2003 | 12744877 |
| cowpea mosaic virus-based chimaeras. effects of inserted peptides on the phenotype, host range, and transmissibility of the modified viruses. | expression of foreign peptides on the surface of cowpea mosaic virus particles leads to the creation of chimaeras with a variety of phenotypes and yields. two factors were shown to be particularly significant in determining the properties of a given chimaera: the length of the inserted sequence and its isoelectric point. the deleterious effect of high isoelectric point on the ability of chimeras to produce a systemic infection occurs irrespective of the site of insertion of the peptide. ultrastr ... | 2003 | 12788630 |
| the molecular basis of the antigenic cross-reactivity between measles and cowpea mosaic viruses. | two nonrelated viruses, cowpea mosaic virus (wtcpmv) and measles virus (mv), were found to induce cross-reactive antibodies. the nature of this cross-reactivity was studied and results are presented here demonstrating that antiserum raised against wtcpmv reacted with peptide from the fusion (f) protein of mv. furthermore, the f protein of mv was shown to share an identical conformational b cell epitope with the small subunit of cpmv coat protein. passive transfer of anti-wtcpmv antibodies into b ... | 2003 | 12788642 |
| the c-terminal region of the movement protein of cowpea mosaic virus is involved in binding to the large but not to the small coat protein. | cowpea mosaic virus (cpmv) moves from cell to cell as virus particles which are translocated through a plasmodesmata-penetrating transport tubule made up of viral movement protein (mp) copies. to gain further insight into the roles of the viral mp and capsid proteins (cp) in virus movement, full-length and truncated forms of the mp were expressed in insect cells using the baculovirus expression system. using elisa and blot overlay assays, affinity purified mp was shown to bind specifically to in ... | 2003 | 12867661 |
| using a quasi-parallel x-ray beam of ultrashort wavelength for high-pressure virus crystallography: implications for standard macromolecular crystallography. | data acquisition from crystals of an icosahedral virus, cowpea mosaic virus (cpmv), was carried out to 2.8 a resolution under an elevated hydrostatic pressure of 330 mpa. this was the first example of a complex macromolecular assembly to be studied by high-pressure crystallography. the data were obtained from the esrf id30 beamline using a quasi-plane wave of ultrashort wavelength with a diamond anvil cell and an imaging-plate detector. the results of the high-pressure data analysis are given an ... | 2003 | 14501116 |
| intracellular distribution of cowpea mosaic virus movement protein as visualised by green fluorescent protein fusions. | cowpea mosaic virus (cpmv) derivatives expressing movement protein (mp) green fluorescent protein (gfp) fusions (mp:gfp) were used to study the intracellular targeting and localization of the mp in cowpea protoplasts and plants. in protoplasts, a virus coding for a wild type mp:gfp (mpfgfp) induced the formation of fluorescent tubular structures, which shows that subcellular targeting and tubule formation are not affected by fusion of gfp to the c-terminus of the mp. in plants, mpfgfp infections ... | 2003 | 14579172 |