Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| ultrastructural comparison of the retroviruses associated with human and simian acquired immunodeficiency syndromes. | three isolates of human acquired immunodeficiency syndrome (aids) virus (lymphadenopathy-associated virus, human t cell lymphotropic virus strain iii, acquired immunodeficiency syndrome-associated retrovirus) were compared with simian aids virus by thin section transmission electron microscopy. simian aids virus was found to form intracytoplasmic a particles and to bud by both ring forms and crescent shapes. the extracellular mature simian aids virus exhibited a cylindrical nucleoid within a tub ... | 1985 | 2991655 |
| isolation of a new serotype of simian acquired immune deficiency syndrome type d retrovirus from celebes black macaques (macaca nigra) with immune deficiency and retroperitoneal fibromatosis. | a new serotype of simian acquired immune deficiency syndrome (saids) retrovirus (type 2) belonging to the d genus of retroviruses is associated with a saids occurring spontaneously in a colony of celebes macaques (macaca nigra) and rhesus macaques (macaca mulatta) at the oregon regional primate research center. this syndrome resembles saids in m. mulatta at the california primate research center, which is associated with a similar type d retrovirus (type 1). however, at the oregon center, saids ... | 1985 | 2997477 |
| molecular cloning of the mason-pfizer monkey virus genome: characterization and cloning of subgenomic fragments. | the molecular characterization of the proviral dna genome of mason-pfizer monkey virus (m-pmv), the prototype d-type retrovirus, is described. an analysis of unintegrated viral dnas present in acutely infected cells revealed open and closed circular molecules and linear species. the size of the m-pmv linear proviral dna is determined to be 8.1 kbp in length. a preliminary screening of restriction enzymes indicated that many of those commonly used for cloning (ecori, sali, clai, xhoi) did not cut ... | 1985 | 2997984 |
| distribution of type d retrovirus sequences in tissues of macaques with simian acquired immune deficiency and retroperitoneal fibromatosis. | horizontally acquired saids retrovirus type 2 (srv-2), a type d retrovirus related to the mason-pfizer monkey virus, has been associated with the simian acquired immunodeficiency syndrome (saids) including retroperitoneal fibromatosis (rf) in several macaque species at two primate research centers. virus specific gene sequences are present in lymphoid and rf tissues but not in muscle tissue of diseased macaques or in any tissues of uninfected normal monkeys. serologic and restriction endonucleas ... | 1986 | 3006332 |
| molecular cloning of the mason-pfizer monkey virus genome: biological characterization of genome length clones and molecular comparisons to other retroviruses. | the molecular cloning of the dna provirus of mason-pfizer monkey virus (m-pmv) is described. fourteen independent clones of integrated m-pmv proviruses were isolated from a human embryo kidney cell line that had been previously derived from a single cell clone infected with m-pmv. characterization of these clones for size of insert, restriction pattern of flanking dna, and presence of repetitive dna in the flanking sequences revealed that 10 of the isolates were identical while the four remainin ... | 1986 | 3016990 |
| immunodeficiency in rhesus monkeys associated with the original mason-pfizer monkey virus. | the mason-pfizer monkey virus (mpmv) was reisolated from a cryopreserved sample of the original mpmv-containing rhesus breast carcinoma, and complete integrated mpmv provirus was detected in chromosomal dna of this tumor. reanalysis of the in vivo pathogenicity and molecular character of mpmv reisolated from the rhesus breast tumor and analysis of the original mpmv after long-term in vitro propagation in human and rhesus cells show that the original mpmv produces an acquired immunodeficiency sim ... | 1986 | 3020302 |
| isolation and characterization of covalently closed circular proviral dna molecules of several type d retroviruses isolated from human cell lines. | infection of a human lymphoblastoid b cell line (raji cells) with type d retroviruses, originally isolated either from subhuman primates (mpmv, lv) or from permanent human cell lines (pmfv, helav, hep-2v) led to the production of type d retrovirus particles. subsequent cocultivation of uninfected and virus-producing raji cells was employed for the generation of sufficient amounts of covalently closed circular dna molecules (cccdna). highest amounts of cccdna were obtained after cocultivation of ... | 1986 | 3024412 |
| morphological changes of an inflammatory myopathy in rhesus monkeys with simian acquired immunodeficiency syndrome. | eleven of 25 rhesus monkeys which died of simian acquired immunodeficiency syndrome (saids) caused by infection with a type d retrovirus related to mason-pfizer monkey virus showed evidence of muscle weakness and atrophy and had elevated levels of muscle enzymes. biopsies of affected muscle studied with enzyme histochemistry showed the characteristic features of polymyositis. inflammatory cells consisting of lymphocytes, macrophages, and large vacuolated bizarre-shaped cells of undetermined type ... | 1987 | 3039516 |
| polypeptides of mason-pfizer monkey virus. ii. synthesis and processing of the env gene products. | mason-pfizer monkey virus (m-pmv), the prototype d-type retrovirus, encodes two glycosylated virion proteins, gp20 and gp70. the polyprotein precursor to these proteins was identified by immunoprecipitation of pulse labeled m-pmv-infected cells with an antiserum raised against gp70, the major glycoprotein of the virus. the relationship of this precursor to the two viral glycoproteins was verified by tryptic peptide mapping, which demonstrated that gp20 and gp70 were independent products of the e ... | 1986 | 3083581 |
| characterization of vero cells. | a cell line used in the production of biologicals should be free of infectious agents, and 'described with respect to cytogenetic characteristics and tumorigenicity'. vero, a continuous cell line derived from a normal african green monkey kidney, was examined for the presence of retroviruses and for tumorigenic potential. we were unable to detect the presence of retroviruses by reverse transcriptase assay, electron microscopy or hybridization of cellular genomic dna with mason-pfizer monkey viru ... | 1988 | 3198660 |
| [antibodies to the structural and nonstructural gag-coded proteins of type-d retroviruses in patients with lymphadenopathy]. | screening of antibodies to structural and nonstructural gag gene-coded proteins in humans with lymphadenopathy and aids was performed by means of radioimmunoprecipitation (rip) and western blotting. pr78gag precursor of gag-coded proteins of type-d retrovirus from hep-2 cells served as an antigen in rip tests. total number of sera (of humans with lymphoadenopathy) under rip analysis was 18 and one sera of aids patient. six of them reacted with pr78gag and one out of one aids serum. over 80 sera ... | 1988 | 3264729 |
| experimental coinduction of type d retrovirus-associated pulmonary carcinoma and lentivirus-associated lymphoid interstitial pneumonia in lambs. | two retrovirus-associated pulmonary diseases of sheep [ovine pulmonary carcinoma (opc); sheep pulmonary adenomatosis], a bronchoalveolar carcinoma, and lymphoid interstitial pneumonia (lip) were induced simultaneously in 9 of 9 neonatal lambs. the lambs were killed 8-28 weeks after intratracheal injection of lung tumor homogenate or lung fluid derived from sheep with naturally occurring opc and ovine lentivirus (ovlv) infection. the inoculated lambs developed multifocal neoplasms of alveolar typ ... | 1987 | 3474445 |
| polypeptides of mason-pfizer monkey virus. iii. translational order of proteins on the gag and env gene specified precursor polypeptides. | mason-pfizer monkey virus (m-pmv), the prototype d-type retrovirus, unlike most retroviruses, preassembles core structures in the cytoplasm of infected cells during morphogenesis. we have shown previously (j. bradac and e. hunter, 1984, virology 138, 260-275) that m-pmv virions contain 5 gag-encoded polypeptides. in this report, the translational order of the proteins encoded on the gag and env genes of m-pmv has been determined by pactamycin mapping. the proposed order of the viral proteins on ... | 1986 | 3485855 |
| myristylation is required for intracellular transport but not for assembly of d-type retrovirus capsids. | the role of myristylation, a fatty acid modification of nascent polypeptides, in the assembly and intracellular transport of d-type retroviral capsids was investigated through the use of oligonucleotide-directed mutagenesis. myristic acid is normally esterified through an amide linkage to a glycine residue at the amino terminus of the mason-pfizer monkey virus gag gene products. mutant pa-1, which has a codon for valine substituted for that of the normally myristylated glycine, is completely non ... | 1987 | 3493352 |
| the localization of a mason-pfizer monkey virus-related antigen in jaagsiekte tumour tissue and cell lines. | mason-pfizer monkey virus-related antigen was detected in 3 out of 5 jaagsiekte lungs examined using a direct immunoperoxidase staining technique with anti-mpmv p27 serum. most of the antigen was localized in the alveolar lumina of the lesions. the reaction was further characterised on immune blots and found to involve a protein with a molecular mass of 29 000 daltons (jsrv p29). jsrv p29 antigen was also detected in 2 jaagsiekte cell lines. | 1986 | 3523361 |
| biochemical and immunological characterization of structural proteins from retrovirus-d/new england and comparison to mason-pfizer monkey virus and permanent human fibroblast virus. | biochemical and immunological properties of retrovirus-d/new england (here referred as r-d/ne) recently isolated at the new england regional primate research center, southborough, ma, from a rhesus monkey with acquired immune deficiency syndrome were investigated and compared to the prototype type d retroviruses mason-pfizer monkey virus (mpmv) and permanent human fibroblast virus (pmfv) isolated from a breast carcinoma of a rhesus monkey and a continuous human cell line, respectively. the polyp ... | 1987 | 3579611 |
| trans-activation of long terminal repeat sequence-mediated gene expression is not a property of type d retrovirus replication. | several retroviruses encode trans-acting factors which activate gene expression directed by long terminal repeat (ltr) sequences and play a role in the positive feedback regulation of virus replication. we have examined two mason-pfizer monkey virus (mpmv) strains for their ability to produce and respond to such factors. plasmids with the ltr of either mpmv or type d retrovirus/new england (d/ne) were fused to the bacterial chloramphenicol acetyltransferase (cat) gene. introduction of these plas ... | 1987 | 3612091 |
| chemical crosslinking of lipids and proteins within mason-pfizer monkey and squirrel monkey type d retroviruses. | nearest neighborhoods between lipids and proteins were investigated in mason-pfizer monkey virus (mpmv) and squirrel monkey retrovirus (smrv) using the chemical crosslinking reagent dimethyl suberimidate (dms). analysis of dms-induced lipid-protein complexes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that in both viruses the major internal structural protein, p27 (mpmv) and p35 (smrv), is linked to the lipid bilayer. additionally, in smrv a second protein (p20) was fou ... | 1986 | 3707293 |
| recombinant human interferons inhibit replication of mason-pfizer monkey virus in primate cells. | pretreatment of mason-pfizer monkey virus-infected human embryonic kidney cells with either of the cloned human interferons, a or a/d, reduces the release of infectious virus particles to the same extent (greater than 97%) as determined by 125i-protein a binding radioimmune assay. while interferon a has no significant effect on the release of infectious viruses from a mason-pfizer monkey virus-infected monkey cell line consistent with the species specificity of this group of compounds, human int ... | 1987 | 3824911 |
| cross-reactivity of a normal human cell surface antigen with primate retrovirus glycoproteins. | normal human cells express a human-specific antigen, huly-m5 (defined by the e4.3 monoclonal antibody), cross-reactive with determinants of the primate retroviruses, mpmv(mason pfizer monkey virus) and galv (gibbon ape leukemia virus). purified virus preparations of mpmv and galv absorbed e4.3 antibody activity while antisera to these retroviruses blocked the binding of e4.3 antibody to human target cells. sequential immunoprecipitation and two-dimensional gel analysis both indicated that the an ... | 1985 | 3924865 |
| purification and n-terminal amino acid sequence comparisons of structural proteins from retrovirus-d/washington and mason-pfizer monkey virus. | a new d-type retrovirus originally designated saids-d/washington and here referred to as retrovirus-d/washington (r-d/w) was recently isolated at the university of washington primate center, seattle, wash., from a rhesus monkey with an acquired immunodeficiency syndrome and retroperitoneal fibromatosis. to better establish the relationship of this new d-type virus to the prototype d-type virus, mason-pfizer monkey virus (mpmv), we have purified and compared six structural proteins from each viru ... | 1985 | 3927012 |
| suppression of human lymphocyte mitogen response by proteins of the type-d retrovirus pmfv. | a type-d retrovirus, derived from a human cell line and designated pmfv, which is related to, but distinct from the mason-pfizer monkey virus (mpmv) suppressed the in vitro mitogen response of human lymphocytes. pmfv was suppressive either as intact virus or after disruption by ether or detergents. in kinetic studies, the time course of the interaction between suppressive virus components and lymphocytes was characterized. neither cytotoxicity nor time shifts in optimum 3h-thymidine incorporatio ... | 1986 | 3943925 |
| retrovirus d/new england and its relation to mason-pfizer monkey virus. | seventeen isolates of retrovirus d/new england have been obtained from three species of macaques at the new england regional primate research center. seven of the isolates were obtained from macaques who subsequently died with the macaque immunodeficiency syndrome; other isolates were obtained from macaques with less severe or other forms of illness. attempts to isolate type d retrovirus from peripheral lymphocytes of 97 apparently healthy macaques have not been successful. cloned dna was prepar ... | 1985 | 3989913 |
| suppression of human lymphocyte mitogen response by retroviruses of type d. ii. non-activity of mason-pfizer monkey virus versus activity of human cell line derived virus pmfv. | in contrast to the human cell line derived type d retrovirus pmfv, the mason-pfizer monkey virus (mpmv) does not suppress the mitogen response of normal human lymphocytes. both viruses have been propagated on the same cell lines and purified by the same methods. mpmv did not contain a factor able to abolish pmfv-induced suppression of the mitogen response. neither could mpmv suppress the mitogen response of lymphocytes from rhesus monkeys or baboons. pmfv however inhibited their reactivity. | 1985 | 4062558 |
| viral and cellular dna polymerase: comparison of activities with synthetic and natural rna templates. | two dna polymerases purified from normal human lymphocytes are distinguishable from the viral reverse transcriptases of avian myeloblastosis virus and mason-pfizer monkey virus by their relative affinity for select templates. in this respect, the activity of the two normal human lymphocyte polymerases closely resembles the activity of escherichia coli dna polymerase 1. the viral and cellular dna polymerases are equally active with the nonspecific template, poly(ra) . poly(dt). criteria for disti ... | 1972 | 4113240 |
| characterization and separation of viral dna polymerase in mouse milk. | two dna polymerase with properties of viral rna-directed dna polymerase can be found in riii mouse milk. one enzyme is the polymerase of type-c viruses; this enzyme prefers manganese to magnesium with poly(ra).oligo(dt) as synthetic template, is inhibited by specific sera, and has an apparent molecular weight of 70,000. milk from balb/c and nih swiss mice contain a vast predominance of this type-c enzyme. the other dna polymerase from riii mouse milk prefers magnesium to manganese, is not inhibi ... | 1973 | 4124683 |
| purification, characterization, and comparison of the dna polymerases from two primate rna tumor viruses. | the dna polymerase from the mason-pfizer monkey virus (m-pmv), an rna tumor virus not typical type-c or type-b, has been purified a thousand-fold over the original crude viral suspension. this purified enzyme is compared to a similarly purified dna polymerase from the primate woolly monkey virus, a type-c virus. the two enzymes have similar template specificities but differ in their requirements for optimum activity. both dna polymerases have a ph optimum of 7.3 in tris buffer. m-pmv enzyme has ... | 1973 | 4127027 |
| mason-pfizer virus characterization: a similar virus in a human amniotic cell line. | mason-pfizer monkey virus (mp-mv) is a rna virus with an rna-instructed dna polymerase first isolated from a rhesus monkey mammary adenocarcinoma in 1970. until recently, there have been no other isolates. a continuous human amnion cell line, ao, was found to be producing a virus indistinguishable or closely related to the mason-pfizer virus as measured by morphological, immunological, and biochemical methods. by thin-section electron microscopy, the extracellular virus particle in ao line is 11 ... | 1973 | 4128382 |
| evidence for allosterism in in vitro dna synthesis on rna templates. | hemoglobin mrna and (ra)(n).(dt)(10) have been used as primer-templates in a kinetic study of dna synthesis with escherichia coli dna polymerase i (dna nucleotidyl transferase, ec 2.7.7.7) and mason-pfizer monkey virus reverse transcriptase (rna-directed dna polymerase). the rate versus enzyme concentration curve is sigmoidal and is consistent with a cooperative phenomenon. the results could be interpreted in terms of the formation of an active complex containing enzyme dimers (or oligomers) on ... | 1974 | 4132534 |
| serological analysis of reverse transcriptase of the mason-pfizer monkey virus. | 1974 | 4133137 | |
| immunological properties of two polypeptides of mason-pfizer monkey virus. | two polypeptides were isolated from the mason-pfizer monkey virus (mp-mv). the polypeptides, designated mp-mv p26 and mp-mv p15, were 26,000 and 15,000 molecular weight, respectively, based on gel filtration chromatography in the presence of 6 m guanidine hydrochloride. radioimmunoassays developed for mp-mv p26 and p15 were, respectively, 10 and 100 times more sensitive than immunodiffusion and 1 and 10 times more sensitive than micro-complement fixation for the detection of mp-mv. antigens of o ... | 1974 | 4134467 |
| plaque assay for the mason-pfizer monkey virus. | 1974 | 4135599 | |
| systematics of rna tumor viruses and virus-like particles of human origin. | [(3)h]dna copies of avian, feline, murine, and primate rna tumor virus genomes were synthesized in vitro by an rna-dependent dna polymerase reaction. these dnas were hybridized to 60-70s rna that had been purified from the viruses. the amount of the [(3)h]dna hybridized yielded a measure of the genetic relatedness among the dna preparations synthesized by the viruses. when many combinations of dna and rna were analyzed, the pattern of hybridization showed in some cases that the dna copies of the ... | 1974 | 4137960 |
| isolation of a new simian foamy virus from a spider monkey brain culture. | a syncytium-forming (foamy) virus was isolated from a spider monkey brain cell culture. cytopathic effect was observed both in the brain culture and in human embryonic kidney cells. neutralizing antibody was present in the sera of the spider monkey from whom the isolation was made. the virus was inhibited by 5-bromo-2-deoxyuridine (20 mug/ml), contained a ribonucleic acid-dependent deoxyribonucleic acid polymerase, and had an infectivity peak at 1.15 g/cm(3) in a sucrose density gradient. the vi ... | 1973 | 4201323 |
| biologic characteristics of transformed rhesus foreskin cells infected with mason-pfizer monkey virus. | 1974 | 4207874 | |
| occurrence of mason-pfizer monkey virus in healthy rhesus monkeys. | 1974 | 4214612 | |
| sequence homology between the rna of mason-pfizer monkey virus and the rna of human malignant breast tumors. | radioactive dna ([(3)h]cdna) complementary to the rna of mason-pfizer monkey virus was used in molecular hybridization experiments to demonstrate sequence homology between its viral rna and rna of human malignant breast tumors. hybridization products were analyzed by cesium sulfate equilibrium density centrifugation and hydroxylapatite chromatography. seven of ten human malignant breast tumors tested contained virus-related rna when assayed by hydroxylapatite chromatography, whereas no sequence ... | 1974 | 4216026 |
| ultrastructural comparison of a virus from a rhesus-monkey mammary carcinoma with four oncogenic rna viruses. | the ultrastructure and morphogenesis of mason-pfizer monkey virus, isolated from a mammary carcinoma in a rhesus monkey, was compared with those of murine mammary tumor virus, murine leukemia virus, l1210 leukemia-associated virus, and avian myeloblastosis virus. the simian virus resembled murine mammary tumor virus and the l1210 virus in that it produced intracytoplasmic particles that were enveloped during budding. it resembled l1210 virus and murine leukemia virus in budding with smooth envel ... | 1971 | 4327008 |
| chronic poliovirus infection of cocultivated monkey cells harboring the mason-pfizer monkey virus. | 1972 | 4345966 | |
| hela-like marker chromosomes and type-a variant glucose-6-phosphate dehydrogenase isoenzyme in human cell cultures producing mason-pfizer monkey virus-like particles. | 1974 | 4472134 | |
| transformation of rhesus foreskin cells by mason-pfizer monkey virus. | 1972 | 4626796 | |
| morphological and biophysical properties of the mason-pfizer monkey virus. | 1972 | 4631464 | |
| mason-pfizer monkey virus isolated from spontaneous mammary carcinoma of a female monkey. i. detection of virus antigens by immunodiffusion, immunofluorescent, and virus agglutination techniques. | 1971 | 4996663 | |
| serological and structural properties of mason-pfizer monkey virus isolated from the mammary tumor of a rhesus monkey. | we describe here serological and structural properties of a virus, mason-pfizer monkey virus (m-pmv), isolated from a simian mammary tumor. this virus is morphologically similar to the known oncogenic rna viruses (oncornaviruses). it has a 60-70s rna, and its replication is inhibited by actinomycin. antisera prepared against the virus isolated by density-gradient centrifugation identify at least two viral structural antigens. immunodiffusion studies show that this virus is serologically unrelate ... | 1971 | 4997759 |
| transmission of simian acquired immunodeficiency syndrome (saids) with type d retrovirus isolated from saliva or urine. | saliva and urine specimens from rhesus monkeys with saids were found to contain a type d retrovirus related to mason-pfizer monkey virus (mpmv) which has been linked etiologically to saids. virus isolates from saliva and urine were shown to have the characteristics of the saids agent by their reverse transcriptase divalent cation preference for synthetic template-primers, production of characteristic cytopathology in raji cells and antigenic relatedness to mpmv as determined by enzyme-linked imm ... | 1984 | 6083568 |
| simian aids--evidence for a retroviral etiology. | this paper reviews the major features of a simian model of acquired immunodeficiency ('saids'), saids occurs endemically in colonies of macaque monkeys in the united states and resembles aids in humans in overall clinical manifestations, pathology, and immune deficiency. an infectious type d retrovirus, related to but distinct from the mason-pfizer monkey virus, has been identified as the primary cause of saids. the relevance of these findings for human aids is discussed. | 1984 | 6090298 |
| experimental infection of rhesus monkeys with type d retrovirus. | the naturally occurring immunodeficiency syndrome of macaque monkeys is an important animal model for the acquired immunodeficiency syndrome in humans. a new type d retrovirus, distinct from mason-pfizer monkey virus, has been isolated from affected animals at the new england regional primate research center. we now report the results of experimental infection of macaques with retrovirus d/new england after 13 months of study. inoculated macaques developed lymphadenopathy without follicular hype ... | 1984 | 6092691 |
| [viral etiologies of aids: facts and hypotheses]. | all the epidemiological features suggest that the acquired immunodeficiency syndrome (aids) is caused by a single transmissible agent and surely a virus. first, cytomegalovirus, epstein-barr virus and hepatitis b virus have been proposed as possible etiological agents of aids. a direct link between ubiquitous viruses and the occurrence of the disease has been discarded. at present time, etiological researches provide evidence that retroviruses are the best candidates for the etiology of aids. th ... | 1984 | 6095408 |
| mason-pfizer monkey virus and simian aids. | 1984 | 6141397 | |
| [detection of a common antigenic determinant in the major inner protein of unrelated oncoviruses]. | a new heterologous system of radioimmunoassay (p24 of bovine leukemia virus--antiserum to rous sarcoma virus) has been developed which demonstrated for the first time the existence of a common antigenic determinant in the major inner protein of unrelated oncoviruses: avian leukemia-sarcoma virus, bovine leukemia virus, mammalian type c viruses (mouse, hamster, monkey) and type d viruses (simian mason-pfizer virus). these data suggest a common origin of unrelated oncoviruses and open new approach ... | 1980 | 6155738 |
| inhibition of mason-pfizer monkey virus-induced syncytium formation in normal human cells by homologous interferon. | 1980 | 6156543 | |
| interrelationship of reverse transcriptases of primate type d retroviruses. | enzyme neutralization experiments by using an antiserum to reverse transcriptase (rt) of mason-pfizer monkey virus (mpmv) demonstrated the presence of common antigenic determinants on the rts of all known type d retroviruses and of determinants discriminating different isolates. the rt of mpmv is antigenically closely related to the rts of type d viruses isolated from human cell lines although significant differences are also detectable. it shows a lesser degree of relationship to the rts of squ ... | 1980 | 6159867 |
| common antigenic determinants on structural proteins p10--12 of pmfv and mpmv type d retroviruses. | pmfv, a type d retrovirus isolated from a malignant human embryo cell line, was compared with mason-pfizer monkey virus (mpmv) in a sensitive tannic acid enhanced indirect immunodiffusion test. in addition to the previously shown common antigens, both viruses contain identical group-specific antigenic determinants on their p 10--12 as demonstrated with a specific p 10--12 mpmv test system. interspecies mammalian type c virus antigens were not detected in highly concentrated pmfv preparations. | 1980 | 6161313 |
| demonstration of antigenic cross-reactivity between simian sarcoma virus (ssv) and mason-pfizer monkey virus (mpmv): brief report. | 1981 | 6171238 | |
| use of sepharose bead immunofluorescence assay for comparison of the type d retroviruses mpmv and pmfv. | mason-pfizer monkey virus (mpmv) and pmfv, an isolate from a human continuous cell line, were compared by sepharose bead immunofluorescence assay. according to the results with p27-specific assays the main structural protein of both viruses seems to be identical in the prominent antigenic determinants. differences were found when comparing the p15s indicating that this viral protein contains type-specific antigenic determinants. | 1982 | 6182856 |
| monoclonal antibodies to three epitopic regions of feline leukemia virus p27 and their use in enzyme-linked immunosorbent assay of p27. | three different monoclonal antibodies were developed against the major core protein (p27) of feline leukemia virus (felv). each antibody was directed against a different epitope of the species-specific portion of felv-p27. the 3 antibodies reacted with 5 different isolates of felv but not with 7 other retroviruses (mulv (rauscher), mulv (akr), mpmv, mmtv, smrv, baev, rd 114). these monoclonal antibodies could readily be adapted to an enzyme-linked immunosorbent assay (elisa) for the specific mea ... | 1983 | 6186744 |
| effect of human interferon on type d retroviruses multiplication in chronically infected cell lines. | a study was made on the effect of human interferon (huifn) on the synthesis and release of type d viruses (mpmv and smrv) by chronically infected human lines. interferon at concentrations of 50 iu/ml lead to an inhibition of the extracellular virus production by about 50%-80% as measured by virus-associated reverse transcriptase activity, by metabolic labeling of the virus with (3h) uridine or (3h) amino acids, or by electron microscopy counting of particles. the profiles of intracellular viral ... | 1983 | 6188793 |
| leukocyte migration inhibition among breast cancer patients in response to various oncogenic viruses. | the direct leukocyte migration inhibition (lmi) test was done with leukocytes from healthy women and from patients with primary operable breast cancer, benign breast disease, or head and neck cancer. purified preparations of murine mammary tumor virus (mumtv), mason-pfizer monkey virus (mpmv), and murine leukemia virus (mulv) were used. for each virus, the lowest 10th percentile of the lmi responses of controls was used to discriminate positive from negative responses. leukocytes from 46 of 94 ( ... | 1980 | 6243720 |
| differential association of transfer rnas with the genomes of murine, feline and primate retroviruses. | the trnas that are bound to the genomic rnas of several murine, feline, and primate retroviruses have been identified. transfer rnas were divided into those loosely bound and those tightly bound by stepwise thermal dissociation of the 70 s rna. they were then identified and semiquantitated by aminoacylation. proline trna is the most tenaciously bound trna in several strains of murine leukemia virus, two strains of feline leukemia virus, and the primate viruses simian sarcoma, baboon endogenous, ... | 1980 | 6248115 |
| fusion of transformed human cells by simian retroviruses. | mason-pfizer monkey virus (mpmv), baboon endogenous virus (baev) and simian sarcoma virus associated virus (ssav) induced fusion in cultured human cells derived from tumors or transformed in vitro. not only virus-transformed cells, but also cells transformed spontaneously by 4-nitroquinoline 1-oxide (4-nqo) treatment or by 60co-gamma ray irradiation were fused by these simian retroviruses. non-transformed cells derived from normal human embryos were not fused by any of these viruses. of these tu ... | 1980 | 6252085 |
| [phorbol ester-induced expression of primate retroviruses (author's transl)]. | addition of the tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate (tpa) to the growth medium of certain human cell cultures persistently infected with simian retroviruses of type c (baev, ssv) or type d (mpmv) or a human cell line-derived type d isolate (pmfv), respectively, resulted in a considerable but transient stimulation of virus production. enhanced virus expression was paralleled by striking morphological alterations of the cells. among four infected cell types tested so far, ony one ... | 1981 | 6279052 |
| dna sequence relationship of the baboon endogenous virus genome to the genomes of other type c and type d retroviruses. | baboon endogenous virus (baev) is a type c retrovirus present in multiple proviral copies in the dna of baboons. although interspecies antigenic determinants present on reverse transcriptase and gag proteins are shared among all mammalian type c viruses, no nucleic acid homology between baev and other type c viruses (except rd-114) has been found in conventional liquid hybridization experiments. in this study, we used restriction fragments of cloned baev dna immobilized on nitrocellulose to test ... | 1982 | 6284972 |
| identification of the rpmi 8226 retrovirus and its dissemination as a significant contaminant of some widely used human and marmoset cell lines. | a retrovirus designated rpmi 8226v, isolated in 1973 from the human myeloma cell line rpmi 8226 has been characterized by competition radioimmunoassay (ria) for the major viral structural protein and by nucleic acid hybridization analysis using cdna of the virus. the virus is highly related to the squirrel monkey type d retrovirus, smrv. in the homologous ria using rabbit anti-rpmi 8226v and 125i-labelled p37 of rpmi 8226v, rpmi 8226v and smrv exhibited competition of 81% and 73% respectively. s ... | 1982 | 6288581 |
| in vivo modification of retroviral gag gene-encoded polyproteins by myristic acid. | it has recently been shown by mass spectral analysis (henderson et al., proc. natl. acad. sci. u.s.a. 80:339-343, 1983) that the p15gag protein of murine leukemia viruses contains a novel post-translational modification, an amino-terminal myristyl (tetradecanoyl) amide. in this report we show that p15gag is the only structural protein to contain this fatty acid. in addition, the gag precursor polyproteins of type b, c, and d retroviruses have been examined for the presence of myristic acid by me ... | 1983 | 6302307 |
| sheep pulmonary adenomatosis: demonstration of a protein which cross-reacts with the major core proteins of mason-pfizer monkey virus and mouse mammary tumour virus. | a retrovirus that causes pulmonary adenomatosis, a contagious lung tumour of sheep, contains a 25 000 mol. wt. polypeptide which cross-reacts with the major core protein (p27) of mason-pfizer monkey virus and mouse mammary tumour virus. | 1983 | 6311958 |
| blastogenic response of lymphocytes derived from patients with hematopoietic malignancies to antigens of a type c retrovirus isolated from a burkitt's lymphoma cell line. | cellular immune response to antigens associated with a type c retrovirus derived from burkitt's lymphoma (bl) lymphoblastoid cells was studied in patients with hematopoietic malignancies, noncancer patients and healthy controls. response was determined by lymphocyte blastogenesis assay measuring [3h]-thymidine incorporation, thereby enabling the calculation of stimulation indices (si). positive response (si greater than 2.0) was demonstrated in patients with multiple myeloma (mm), chronic lympho ... | 1983 | 6315444 |
| polypeptides of mason-pfizer monkey virus. i. synthesis and processing of the gag-gene products. | mason-pfizer monkey virus (m-pmv), the prototype d-type retrovirus, differs from the mammalian c-type retroviruses by preassembling core structures in the cytoplasm of infected cells during morphogenesis. studies that define the protein composition of m-pmv virions and identify two gag-related polyprotein precursors in m-pmv infected cells are reported. the polyprotein precursor to the internal structural (gag) proteins of m-pmv was identified by immunoprecipitation from lysates of pulse-labeled ... | 1984 | 6333757 |
| a new type d retrovirus isolated from macaques with an immunodeficiency syndrome. | macaque monkeys with the recently described acquired immunodeficiency syndrome show a marked defect in t-lymphocyte function and die with opportunistic infections and lymphoproliferative abnormalities. in the study described here a new type d retrovirus was isolated from two macaca cyclopis with this syndrome. this virus is related to, but distinct from, mason-pfizer monkey virus, a type d retrovirus previously isolated from a mammary tumor of a rhesus monkey (macaca mulatta). | 1984 | 6695172 |
| simian aids: isolation of a type d retrovirus and transmission of the disease. | a type d retrovirus related to but distinct from mason-pfizer monkey virus was isolated in vitro from the blood of two rhesus monkeys (macaca mulatta) with simian acquired immunodeficiency syndrome (saids). three juvenile rhesus monkeys that were injected intravenously with tissue culture fluids containing this virus developed saids after 2 to 4 weeks. | 1984 | 6695196 |
| detection of type d retrovirus in a human amnion cell line (fl). | this paper describes the detection of viral particles in a human amnion cell line (fl). these particles belong to the group of type d retroviruses, because of their characteristic morphology, their major antigenic determinants, and the presence of particle-associated mg++-preferring rna-dependent dna polymerase. in addition to budding and mature type d virus particles, intracytoplasmic type a structures have also been found. immunological analysis provided evidence of cross-reactivity between th ... | 1980 | 6784700 |
| transfection with the proviral dna of a type d retrovirus (mason-pfizer monkey virus). | chromosomal dna was isolated from cmmt cells which were producing mason-pfizer monkey virus (m-pmv), a type d simian retrovirus. human embryonic cells were transfected with the dna. about 2 weeks after transfection, 3 of 7 culture dishes began to produce m-pmv which could be detected by virus-mediated cell fusion in rsb human cells dually transformed by rous sarcoma virus and simian virus 40, reverse transcriptase assay, and electron microscopy. mock-transfected cultures did not produce virus. | 1982 | 6811499 |
| isolation of a unique retrovirus, mnv-1, from macaca nemestrina. | cell cultures established from the spleen of a macaca nemestrina with enzootic retroperitoneal fibromatosis (erf) spontaneously released a unique retrovirus. throughout 14 serial passages, the spleen cell cultures remained fibroblastic and no cytopathic effect was evident. the virus incorporates [3h]uridine, contains an rna-dependent dna polymerase (rddp), has a buoyant density of 1.15 g/cm3 in sucrose, and was designated mnv-1. virion-associated reverse transcriptase showed no preference for ei ... | 1983 | 6868369 |
| chemical crosslinking of major structural proteins within type d retroviruses. | the nearest neighbor relationships of major structural proteins within type d retroviruses (smrv, mpmv, pmfv) were investigated by crosslinking with the cleavable bifunctional reagent dimethyl dithiobispropionimidate (dtbpi) or by use of 2-iminothiolane (methyl mercaptobutyrimidate, mbi) and subsequent oxidation by hydrogen peroxide. the crosslinked complexes of proteins were analyzed by two-dimensional diagonal polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. in int ... | 1983 | 6882406 |
| feasibility studies of oncornavirus production in microcarrier cultures. | studies conducted with virus-infected monolayer cell cultures have demonstrated the feasibility of producing several tumor-associated viruses in microcarrier (mc) cultures (sephadex g50 beads treated with deae-chloride). the efficiency of cell adherence to mc varied with the cell type, the ph of the growth medium, and the stirring force applied to keep the mc in suspension. most cells attached firmly to the mc and could not be removed easily with routine trypsinization procedures. techniques usi ... | 1980 | 6993346 |
| tumor promoter-stimulated synthesis of mason-:pfizer monkey virus. | the tumor promoter 12-0-tetradecanoyl-phorbol-14-acetate (tpa) increases by severalfold the synthesis of mason-pfizer monkey virus (mpmv), a type d retrovirus, when the virus is growing in human embryo kidney (hek) cells. the effect is transient and paralleled by a striking morphological alteration of the cells. the optimal tpa concentration for stimulation is 5 ng. ml-1. contrary to infected hek cells, tpa induces at similar concentrations neither stimulation of mpmv synthesis nor altered morph ... | 1982 | 7061574 |
| effect of monensin on mason-pfizer monkey virus glycoprotein synthesis. | the effect of the monovalent carboxylic ionophore monensin on the biosynthesis, intracellular transport, and surface expression of the glycoproteins of mason-pfizer monkey virus was examined. cells treated with monensin at concentrations of 10(-7) or 10(-6) m continued to synthesize virus particles, which from electron microscopic studies appeared to bud normally from the plasma membrane of the cells. however, the particles released had an altered buoyant density in sucrose gradients and were no ... | 1982 | 7176016 |
| effect of tunicamycin on cell fusion induced by mason-pfizer monkey virus. | mason-pfizer monkey virus, a d-type retrovirus, has been shown to induce multinucleate cell (syncytium) formation or cell fusion in several normal primate cells. a series of experiments has been carried out to examine whether a glycosylated "fusion-inducing" product is responsible for this biological property of mason-pfizer monkey virus. treatment of rhesus monkey fetal lung cells with different concentrations of tunicamycin, a potent inhibitor of glycosylation, during infection with mason-pfiz ... | 1981 | 7241666 |
| type d retroviruses from human cells do not contain a minor glycoprotein shared by old world monkey type d viruses. | the protein patterns of type d retroviruses isolated either from permanent human cells (hela virus, hela v, and hep-2 virus, hep-2v) or from spectacled langur (langur virus, lv), an old world monkey, were investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis. labeling with 14c-amino acids and 3h-glucosamine revealed for each of these isolates five polypeptides with molecular weights of 10,000, 12,000, 15,000, 25,000 and about 68,000 and identified the 68,000 d-protein as a ... | 1981 | 7332488 |
| the protein pattern of pmf virus, a type-d retrovirus from malignant permanent human cell lines. | the protein pattern of a type-d retrovirus (pmfv) isolated from and propagated in human cell lines has been investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). staining with coomassie blue and labeling with 14c-leucine/14c-lysine revealed five viral polypeptides with molecular weights of 10,000, 12,000, 15,000, 25,000, and 68,000. the 68,000 d-protein was shown to be a glycoprotein by incorporation of 3h-glucosamine and the 15,000 d-protein was identified as ... | 1980 | 7387404 |
| stem cell factor binding to retrovirus primer binding site silencers. | using modified nuclear lysis and binding conditions, we have examined the binding of an embryonal carcinoma (ec) cell factor, binding factor a, to a stem cell-specific silencer which acts at the dna level and overlaps the moloney murine leukemia virus (m-mulv) proline primer binding site (pbs). following our protocol, we found that in vitro binding of factor a correlated with the in vivo activity of the m-mulv silencer. factor a bound specifically to the wild-type silencer element at room temper ... | 1995 | 7529329 |
| production of soluble surface glycoprotein, gp70, of the mason-pfizer monkey virus in cos cells is dependent on the presence of regions of the transmembrane domain. | 1995 | 7672366 | |
| cloning, bacterial expression, and characterization of the mason-pfizer monkey virus proteinase. | we have cloned and expressed the 3' region of the mason-pfizer monkey virus pro gene in escherichia coli. the recombinant 26-kda precursor undergoes rapid self-processing both in e. coli and in vitro at the nh2 terminus, yielding a proteolytically active 17-kda protein, p17. this initial cleavage is followed in vitro by a much slower self-processing that leads to emergence of proteolytically active p12 and a cooh-terminal cleavage product p5. we have found the nh2-terminal processing site of bot ... | 1995 | 7797487 |
| efficient in vivo and in vitro assembly of retroviral capsids from gag precursor proteins expressed in bacteria. | the capsid precursor protein (gag) of mason-pfizer monkey virus, the prototype type d retrovirus, has been expressed to high levels in bacteria under the control of the phage t7 promoter. electron microscopic studies of induced cells revealed the assembly of capsid-like structures within inclusion bodies that formed at the poles of the cells 6 h after induction with isopropyl-beta-d-thiogalactopyranoside (iptg). the inclusion bodies and enclosed capsid-like structures were solubilized completely ... | 1995 | 7815488 |
| characterization of infectious type d retrovirus from baboons. | infectious virus resembling type d simian retrovirus (srv) was isolated from ethiopian baboons (papio cynocephalus) (srv-pc) housed at the university of washington regional primate research center. when baboon peripheral blood mononuclear cells (pbmc) or tissues were cocultured with the h-9 human t-cell line or the raji human b-cell line, large multinucleated syncytia positive for srv-2 antigens were observed microscopically. immunoblot analysis of purified srv-pc from cell culture supernatants ... | 1995 | 7871741 |
| proteolytic processing of particle-associated retroviral polyproteins by homologous and heterologous viral proteinases. | retroviral proteinase(pr)-catalyzed cleavage of the viral gag and gag-pol polyproteins within the nascent virus particle is required for productive viral infection. kinetic characterization and specificity analyses have been reported for several retroviral pr using oligopeptide substrates. in this study, we performed a comparative analysis of pr from avian, bovine, simian and human retroviruses using polyproteins of human immunodeficiency virus (hiv) type 1 or avian leukosis virus as substrates. ... | 1995 | 7883003 |
| secondary structure model of the mason-pfizer monkey virus 5' leader sequence: identification of a structural motif common to a variety of retroviruses. | a stable secondary structure model is presented for the region 3' of the primer-binding site to 130 bases into the gag sequence of the prototype type d retrovirus mason-pfizer monkey virus. using biochemical probing of rna from this region in association with free energy minimization, we have identified a stem-loop structure in the region, which from other studies has been shown to be important for genomic rna encapsidation. the structure involves a highly stable stem of five g-c pairs terminati ... | 1995 | 7884866 |
| continuous propagation of rre(-) and rev(-)rre(-) human immunodeficiency virus type 1 molecular clones containing a cis-acting element of simian retrovirus type 1 in human peripheral blood lymphocytes. | molecular clones of human immunodeficiency virus type 1 that contained either 37 point mutations in the rev-responsive element (rre) that did not affect the overlapping env reading frame or both a mutated rre and two mutations that eliminated rev were constructed. the mutations in the rre were shown to remove both negative and rev-inducible positive effects of the rre on gene expression (g. nasioulas, a. s. zolotukhin, c. tabernero, l. solomin, c. p. cunningham, g. n. pavlakis, and b. k. felber, ... | 1994 | 7966585 |
| mutations within the transmembrane glycoprotein of mason-pfizer monkey virus: loss of su-tm association and effects on infectivity. | we have previously reported that an 11-amino-acid deletion (d33) within the immunosuppressive peptide (isp) region of the mason-pfizer monkey virus transmembrane (tm) glycoprotein, gp22, caused the loss of interaction between tm and the surface (su) glycoprotein, gp70. this resulted in the secretion of large amounts of biologically active su glycoprotein, and we postulated that the isp might represent a point of contact between the two glycoproteins. to further define the amino acids that might ... | 1994 | 8030233 |
| a small element from the mason-pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication rev-independent. | replication of human immunodeficiency virus type 1 (hiv-1) is dependent on the viral rev protein. this protein acts in concert with the cis-acting rev-responsive element present in intron-containing rnas to facilitate nuclear export of these rnas. here we show that a cis-acting 219-nucleotide sequence from an unrelated "simple" retrovirus, mason-pfizer monkey virus (mpmv), enables rev-independent hiv-1 replication. this sequence is present in an untranslated region near the 3' end of the mpmv ge ... | 1994 | 8108397 |
| serological survey for type d retroviruses and simian immunodeficiency virus in monkeys living in hungary. | acquired immunodeficiency in non-human primates has been associated with two types of retroviruses: (i) simian retrovirus (srv), a type d virus and (ii) a lentivirus, simian immunodeficiency virus (siv). both types represent a serious health threat to animals living in different primate research centres, zoos, medical institutions and in private homes. the sera of 175 monkeys belonging to different species were tested for the presence of antibodies against type d retroviruses and siv. the mason- ... | 1993 | 8116496 |
| postassembly cleavage of a retroviral glycoprotein cytoplasmic domain removes a necessary incorporation signal and activates fusion activity. | viral protease-mediated cleavage within the cytoplasmic domain of the transmembrane (tm) glycoprotein of the type d retrovirus, mason-pfizer monkey virus, removes approximately 16 amino acids from the carboxy terminus of the protein. to determine the functional significance of this cleavage in the virus life cycle, we introduced premature stop codons into the tm coding domain, resulting in the production of truncated glycoproteins. progressive truncated of the cytoplasmic domain identified the c ... | 1994 | 8207836 |
| retroviral nucleocapsid proteins possess potent nucleic acid strand renaturation activity. | the nucleocapsid protein (nc) is the major genomic rna binding protein that plays integral roles in the structure and replication of all animal retroviruses. in this report, select biochemical properties of recombinant mason-pfizer monkey virus (mpmv) and hiv-1 ncs are compared. evidence is presented that two types of saturated zn2 nc-polynucleotide complexes can be formed under conditions of low [nacl] that differ in apparent site-size (n = 8 vs. n = 14). the formation of one or the other compl ... | 1993 | 8443601 |
| expression of simian type d retroviral (mason-pfizer monkey virus) capsids in insect cells using recombinant baculovirus. | mason-pfizer monkey virus (m-pmv) is a primate retrovirus that shows type d morphogenesis in mammalian cells. immature intracytoplasmic a type particles (icaps) preassemble in the infected cell cytoplasm migrate to the plasma membrane and are released by budding. this is in contrast to retroviruses that show type c morphogenesis, where assembly and budding occur concurrently at the plasma membrane. we expressed the m-pmv structural genes (gag-pro-pol) in insect cells using a recombinant baculovi ... | 1993 | 8517023 |
| development and application of an antibody elisa for the marker protein of ovine pulmonary carcinoma. | ovine pulmonary carcinoma (opc) is a contagious pulmonary neoplasia with a suspected retroviral etiology. the major core protein (p27) of the putative opc virus cross-reacts with antibodies to p27 of the mason-pfizer monkey virus (mpmv), a type-d retrovirus. this serological reactivity serves as the only accepted biological marker for opc. in order to make a useful reagent for the detection of the opc marker for serodiagnosis and epidemiological studies, the mpmv-p27 coding region was cloned and ... | 1995 | 8571550 |
| presence of mason-pfizer monkey virus in some stocks of the human hbl-100 mammary epithelial cell line. | 1996 | 8609647 | |
| synthesis and assembly of retrovirus gag precursors into immature capsids in vitro. | the assembly of retroviral particles is mediated by the product of the gag gene; no other retroviral gene products are necessary for this process. while most retroviruses assemble their capsids at the plasma membrane, viruses of the type d class preassemble immature capsids within the cytoplasm of infected cells. this has allowed us to determine whether immature capsids of the prototypical type d retrovirus, mason-pfizer monkey virus (m-pmv), can assemble in a cell-free protein synthesis system. ... | 1996 | 8648705 |
| the posttranscriptional control element of the simian retrovirus type 1 forms an extensive rna secondary structure necessary for its function. | it was previously shown that a 240-nucleotide (nt) rna element (cis-acting transactivation element [cte]) located between the env gene and the 3' long terminal repeat of simian retrovirus type 1 (srv-1) can functionally replace posttranscriptional activation directed by rev and the rev-responsive element (rre) when inserted into a rev- and rre-deficient molecular clone of human immunodeficiency virus type 1, resulting in efficient virus replication. here, we analyze the molecular and structural ... | 1996 | 8709222 |
| rev/rre-independent mason-pfizer monkey virus constitutive transport element-dependent propagation of sivmac239 vectors using a single round of replication assay. | in a step toward creating live-attenuated or dna subunit vaccines for aids, the replication of simian immunodeficiency virus (siv) was studied independently of the rev and rre (rev-responsive element) regulatory system, over a single round. to accomplish this, the env gene of an siv vector was made defective by the insertion of a sv40 promoter/enhancer hygromycin b phosphotransferase gene cassette. using this vector as the backbone, molecular clones of siv were generated that contained a mutated ... | 1996 | 8806531 |
| role of mason-pfizer monkey virus (mpmv) constitutive transport element (cte) in the propagation of mpmv vectors by genetic complementation using homologous/heterologous env genes. | to study mason-pfizer monkey virus (mpmv) replication over a single round, virus particles were generated that contain a replication-defective vector encoding a dominant selectable marker, the hygromycin b phosphotransferase (hyg) gene. genetic complementation with a homologous mpmv envelope glycoprotein (env-gp) or pseudotyping by several heterologous env-gps from a variety of viruses resulted in infectious mpmv particles containing the replication-defective rna. recently, it has been shown tha ... | 1996 | 8874512 |
| type d retrovirus markers in healthy africans from guinea. | sixteen matching sera and dna samples from healthy african blood donors living in rural areas of guinea were analysed for the presence of type d retrovirus markers. screening for the antibodies against structural proteins of mason-pfizer monkey virus (m-pmv) was carried out by western blot with a purified m-pmv as an antigen. eight out of 16 sera samples were found to contain antibodies against at least two gag gene-coded proteins, and three of these were weakly positive against env gene-coded p ... | 1996 | 8958587 |