Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [comparative evaluation of the antiviral efficacy of virazole and ribamidil in experimental lassa fever in monkeys]. | lassa virus administered to monkeys aerogenically induced in these laboratory animals a fatal disease modelling the severe course of human lassa fever. it was demonstrated experimentally that ribamydil, a soviet analogue of virasol, as well as foreign commercial virasol was therapeutically effective in experimental lassa fever in aerogenically infected monkeys. | 1990 | 2389569 |
| expression of the lassa virus nucleocapsid protein in insect cells infected with a recombinant baculovirus: application to diagnostic assays for lassa virus infection. | the coding region of the gene for the nucleocapsid protein of lassa virus has been inserted into the genome of autographa californica nuclear polyhedrosis virus (acnpv) using the transfer vector pacym1, so that expression of the foreign dna is under the control of the promoter of the acnpv polyhedrin gene. infection of cultured spodoptera frugiperda cells with recombinant virus resulted in the synthesis of high levels of a protein that was indistinguishable from the authentic lassa virus protein ... | 1990 | 2406367 |
| [characteristics of monoclonal antibodies against lassa virus]. | some properties of monoclonal antibodies to the lassa virus have been characterized. the competitive immunoenzyme analysis has revealed the presence of at least three antigens in the lassa virus nucleoprotein. | 1987 | 2441254 |
| fine mapping of a peptide sequence containing an antigenic site conserved among arenaviruses. | the lymphocytic choriomeningitis virus (lcmv) structural glycoproteins gp-1 (mr 44k) and gp-2 (mr 35k) are encoded on a single intracellular proteolytic cleavage precursor glycoprotein, gp-c (mr 76k). we have used a series of synthetic peptides derived from the deduced amino acid sequence of lcmv gp-c to define an antigenic site containing two topographically overlapping epitopes. three mouse monoclonal antibodies directed against two epitopes on gp-2 were assayed for binding in solution phase b ... | 1988 | 2452517 |
| [characteristics of the immune response in mice immunized with the inactivated lassa virus]. | the influence of humoral and cell-mediated immunity on the formation of resistance in mice immunized with inactivated lassa virus was evaluated. as a result of immunization, both nonspecific and specific immune response developed, the main rule in the formation of resistance belonging to specific immunity. | 1989 | 2474905 |
| epitope mapping of the lassa virus nucleoprotein using monoclonal anti-nucleocapsid antibodies. | monoclonal antibodies with differing specificity were prepared against the josiah strain of the lassa virus. all monoclonal antibodies were characterized by subclass determination and the immunofluorescence test against lassa, lcm (we & arm strain), junin, machupo, and other arenavirus antigens. in radioimmune precipitation tests using purified lassa virus antigen all monoclonal antibodies precipitated a single band of 60 kd, specific for the viral nucleoprotein (p 60). three domains (a, b, c) w ... | 1989 | 2476109 |
| antibody prevalence against haemorrhagic fever viruses in randomized representative central african populations. | between 1985 and 1987, 5,070 randomly selected persons living in 6 central african countries (cameroon, central african republic, chad, congo, equatorial guinea and gabon) were checked for serological evidence of haemorrhagic fever. rural and urban areas were studied, including ecoclimatic zones ranging from dry savana to tropical rain forest. virus-reactive antibodies were found with all antigens tested, and the global prevalence of positive sera was distributed as follows: crimean-congo haemor ... | 1989 | 2505350 |
| antibodies to haemorrhagic fever viruses in madagascar populations. | sera of 381 adult people from 5 areas in madagascar were tested by the indirect immunofluorescence method for antibodies against congo-crimean haemorrhagic fever and rift valley fever viruses (bunyaviridae), ebola (strains zaire and sudan) and marburg viruses (filoviridae), and lassa virus (arenaviridae). the highest prevalence rate was that of ebola virus (4.5%). as no haemorrhagic syndrome has been found associated with this virus, the possible presence of a less pathogenic, antigenically rela ... | 1989 | 2515626 |
| preparation and biochemical characterization of lassa virus subvirion fractions. | 14c-labelled lassa virus was purified by isopycnic centrifugation and split to subvirion fractions. the purified virus was treated with nonionic detergents nonidet p-40 (np-40) and with octylglycoside. after ultracentrifugation in urographin density gradient, two subvirion fractions with buoyant density of 1.24-1.26 and 1.08-1.10 g/cm3 were obtained. the first fraction corresponded to the nucleocapsid of lassa virus: it contained a protein with molecular mass of 60 kda, the l and s segments of t ... | 1989 | 2569820 |
| identification of lassa fever virus infection with recombinant nucleocapsid protein antigen. | 1989 | 2572935 | |
| [lassa and mozambique viruses: cross protection in experiments on mice and action of immunosuppressants on experimental infections]. | the pathogenicity of mozambique virus for random-bred mice and cba mice was studied. in contrast to lassa virus, intracerebral inoculation of newborn zandom-bred mice with mozambique virus (1000 pfu/mouse) results in death of the animals. the pathogenic properties of both viruses for adult cba mice were found to be similar: intracerebral inoculation of the viruses caused death of the animals within 6-8 days, but not intraperitoneal inoculation. with the latter, they produced a population of immu ... | 1989 | 2609647 |
| [dynamics of antigen and antibody accumulation in adult mice, infected with lassa virus]. | 1989 | 2658319 | |
| replication of hemorrhagic fever viruses in monocytic cells. | monocytes play a central role in protection against many viruses. in some infections they are target cells for viral replication. there is increasing evidence that these cells may also be important in regulation of hemostasis. the part played by monocytic cells in the pathogenesis of hemorrhage in the viral hemorrhagic fevers is presently uncertain. monocytes and monocytic cell lines have been used to investigate the ability of viruses to infect these cells in vitro. several factors may affect t ... | 1989 | 2665010 |
| [the first imported case of lassa fever in japan]. | 1989 | 2724572 | |
| protection of guinea pigs from lassa fever by vaccinia virus recombinants expressing the nucleoprotein or the envelope glycoproteins of lassa virus. | a recombinant vaccinia virus that expresses the nucleoprotein gene of lassa virus (josiah strain) under the control of the p7.5 promoter was constructed using the lacz coexpression transfer vector psc11. southern blot analysis demonstrated that recombination of the sequences inserted within the thymidine kinase gene of the transfer vector into the hindiii j fragment of vaccina virus genomic dna occurred properly. a 63-kda protein identical in electrophoretic mobility to authentic lassa nucleopro ... | 1989 | 2741340 |
| [effect of immunosuppression on the development and outcome of an acute infection in mice caused by administration of the lassa virus]. | in experimental infection of mice with lassa virus, the infectious virus could be detected in all the organs and brain tissues tested. histopathological lesions were demonstrated in cerebral and spinal cord tissues only. roentgen irradiation in a dose of 500 r and cyclophosphamide protected mice against a lethal lassa virus dose. cyclosporin a in various doses exerted no effect on the outcome of the acute infection. the adoptive transfer of splenocytes from mouse donors inoculated intraperitonea ... | 1989 | 2763513 |
| a plasma inhibitor of platelet aggregation in patients with lassa fever. | previous studies have shown that haemorrhage in lassa fever is associated with abnormal in vitro platelet aggregation and a high mortality. in sierra leone we studied platelet aggregation in healthy local subjects, patients with laboratory-confirmed lassa fever and febrile patients in whom lassa virus infection was excluded. there were no significant differences in the mean platelet counts of these groups. patients with fulminant lassa virus infection showed a gross depression of in-vitro platel ... | 1989 | 2775659 |
| [pathogenic machupo and lassa arenaviruses: the biochemical properties of virion rna and proteins]. | lassa virus purified in the isodensity sucrose concentration gradient had the following buoyant densities: 1.17 g/cm3 (sucrose), 1.19 g/cm3 (cesium chloride), 1.16 g/cm3 (urografin). similar parameters were obtained for machupo virus. virion rnas of these viruses contained 5 sedimentation classes of molecules: 30-31s, 28s, 22-24s, 18s, and 4-6s. experiments on hybridization of individual sedimentation classes of rna with an excess of poly(a)-containing rna from the infected cells as well as inhi ... | 1987 | 2825435 |
| [identification of the mrna of the nucleocapsid proteins of pathogenic arenaviruses]. | total rna from cells infected with machupo and lassa viruses as well as individual sedimentation classes of these rnas were translated in the cell-free protein-synthesizing system from rabbit reticulocytes. the translation products were precipitated either with anti-machupo immune gamma-globulin or monoclonal antibodies to nucleocapsid protein (np) of lassa virus. both total rna and rna fraction with the sedimentation coefficient 15-16s promoted the synthesis of protein which comigrated in gel w ... | 1987 | 2829439 |
| [template activity of poly(a+) and poly(a-) messenger rna of pathogenic arenaviruses]. | the total rna from cells infected with machupo and lassa viruses as well as poly(a+) and poly(a-) fractions of the rna were translated in the cell-free protein synthesizing system from rabbit reticulocytes. the translated products were treated with specific antibodies and analyzed in polyacrylamide gel electrophoresis. only poly(a-) fraction of rna coded for the synthesis of np protein in vitro. the mrnas for np protein of machupo and lassa viruses are supposed to contain no poly(a) sequences at ... | 1988 | 2848192 |
| early diagnosis of human lassa fever by elisa detection of antigen and antibody. | sequential serum samples, beginning on the day of hospital admission, from three patients with lassa fever were tested for the presence of lassa-virus antigens and antibodies by means of an enzyme-linked immunosorbent assay. lassa-virus antigens were detected in the first serum sample from each patient, thus providing an early definitive diagnosis. in contrast, seroconversion was not detectable by elisa or indirect fluorescent antibody techniques until 3 days or more after admission. the antigen ... | 1985 | 2857321 |
| identification of ippy as a lassa-fever-related virus. | 1985 | 2857974 | |
| sedimentation analysis of the rnas isolated from interfering particles of lassa and machupo viruses. | vero cells chronically infected with lassa virus as well as bhk-21 cells chronically infected with machupo virus produced particles interfering with the replication of homologous, but not heterogeneous viruses. rnas of standard virus particles contained 4 sedimentation classes of molecules: 28-31 s, 22-24s, 18 s and 4-6 s. the 28-31 s molecules were not present among the rnas of interfering particles of lassa and machupo viruses. | 1985 | 2869655 |
| no evidence for increased risk of lassa fever infection in hospital staff. | a prospective serological study was undertaken in hospital personnel who care for lassa fever (lf) patients in an endemic region of sierra leone, west africa. among personnel from three hospitals where barrier nursing is practised, antibody prevalence and seroconversion by age and sex were consistently equal to or lower than those of persons in nearby village populations. no group among hospital personnel evaluated by age, sex, contact, or occupational exposure was at higher risk than another. h ... | 1986 | 2877335 |
| vaccinia recombinant expressing lassa-virus internal nucleocapsid protein protects guineapigs against lassa fever. | the lister strain of vaccinia virus was used to construct a recombinant that expressed the nucleocapsid gene of lassa virus. guineapigs immunised with the recombinant virus were protected against challenge with lassa virus, whereas control animals showed the usual disease course, including pyrexia, anorexia, viraemia, and death. these data indicate not only that protective immunity to lassa fever can be evoked by an experimental vaccine, but also that this response can be mediated by an internal ... | 1987 | 2885642 |
| differentiation between two african arenaviruses (lassa and mozambique) by plaque assay. | the biological characteristics of lassa and mozambique viruses were compared by plaque assay. both viruses produced plaques under the same conditions, however, lassa virus plaques measured on average 2 mm in diameter being twice as large as the mozambique virus plaques. by plaque reduction tests, cross protection was demonstrated between both viruses showing that lassa and mozambique viruses are distinct but related. | 1987 | 2886023 |
| identification of messenger rna for nucleocapsid protein of lassa virus in infected cells. | single-stranded rnas from lassa virus-infected cells were fractionated by affinity chromatography on an oligo(dt)-cellulose column as well as by sucrose gradient centrifugation. fractionated rnas were translated in rabbit reticulocyte lysates, and translation products were precipitated with monoclonal antibodies to the nucleocapsid protein of lassa virus. it has been shown that mrna for the nucleocapsid protein is 15-16s and the rna does not contain long if any poly(a) sequences. | 1988 | 2900821 |
| protection of rhesus monkeys from fatal lassa fever by vaccination with a recombinant vaccinia virus containing the lassa virus glycoprotein gene. | lassa fever is an acute febrile disease of west africa, where there are as many as 300,000 infections a year and an estimated 3000 deaths. as control of the rodent host is impracticable at present, the best immediate prospect is vaccination. we tested as potential vaccines in rhesus monkeys a closely related virus, mopeia virus (two monkeys), and a recombinant vaccinia virus containing the lassa virus glycoprotein gene, v-lsgpc (four monkeys). two monkeys vaccinated with the new york board of he ... | 1989 | 2911575 |
| nucleotide sequence of the lassa virus (josiah strain) s genome rna and amino acid sequence comparison of the n and gpc proteins to other arenaviruses. | the complete nucleotide sequence of the s genome rna of the josiah strain of lassa virus was determined from cloned cdna. the s rna is 3402 nucleotides long with a calculated molecular weight of 1.09 x 10(6) da. the nucleotide base composition is 26.84% adenine, 21.40% guanine, 22.75% cytosine, and 29.01% uridine. the 5' and 3' terminal nucleotide sequences are conserved and complimentary for 19 nucleotides, the nucleoprotein and glycoprotein genes are arranged in ambisense coding strategy, and ... | 1989 | 2916333 |
| early events in arenavirus replication are sensitive to lysosomotropic compounds. | lysosomotropic compounds (ammonium chloride, chloroquine, amantadine, monensin) effectively inhibited the replication of pichinde, mopeia, and lassa viruses in bhk-21 and vero cells. the inhibitory effect was dependent upon the time of drug addition and was most effective when the drugs were added 1 h before the viral adsorption. the drugs had no direct effect on the infectious viruses nor on adsorption of the arenaviruses. these results suggest that the arenaviruses enter cells by adsorptive en ... | 1989 | 2923547 |
| antibodies to lassa and lassa-like viruses in man and mammals in the central african republic. | 715 human sera from different locations in the central african republic were tested by indirect immunofluorescence against the three african arenaviruses, lassa, mobala and mopeia. four were positive for lassa, seven for mobala, and one for both lassa and mopeia. the epidemiology of african arenaviruses is discussed. | 1985 | 2986324 |
| studies of the coagulation system in arenaviral hemorrhagic fever: experimental infection of strain 13 guinea pigs with pichinde virus. | significant coagulation abnormalities were associated with experimental infection of strain 13 guinea pigs with pichinde virus, an arenavirus related to the virulent human pathogens junin, machupo, and lassa viruses. infected animals developed decreased activity of multiple coagulation factors, decreased antithrombin iii levels, high levels of fibrin-fibrinogen degradation products, impaired platelet function, and thrombocytopenia. testing for the presence of a coagulation inhibitor revealed a p ... | 1987 | 3030150 |
| hemorrhagic fever virus infections in an isolated rainforest area of central liberia. limitations of the indirect immunofluorescence slide test for antibody screening in africa. | serum samples from 119 healthy individuals and 106 epilepsy patients inhabiting grand bassa county, liberia, were tested for antibodies to hemorrhagic fever viruses (hfv) by indirect immunofluorescence. e6 vero cells infected with lassa fever virus (las), rift valley fever virus (rvf), congo hemorrhagic fever virus (con), marburg virus (mbg) and the ebola (ebo) virus strains mayinga (may) and boniface (bon) were used as antigen. to obtain reproducible and specific test results sera had to be abs ... | 1986 | 3092415 |
| viral hemorrhagic fever antibodies in nigerian populations. | using the immunofluorescence test, a serosurvey for antibodies to five viral agents associated with hemorrhagic febrile infections was conducted with 1,677 human sera from different parts of nigeria. three hundred fifty-seven (21.3%) were positive for lassa virus antibody, while antibodies to rift valley fever virus were detected in 42 (2.5%) of the sera. testing for rift valley fever virus antibody was confirmed by plaque reduction neutralization test. antibodies to ebola and marburg viruses we ... | 1988 | 3128130 |
| lassa fever: epidemiology, therapy and vaccine development. | 1988 | 3138361 | |
| genetic variation among lassa and lassa-related arenaviruses analysed by t1-oligonucleotide mapping. | total rna and small rna species of several african arenavirus strains have been studied by t1-oligonucleotide mapping. genetic heterogeneity is observed and discussed on the basis of evolutionary biology of the lassa complex. | 1988 | 3145759 |
| [the indirect hemagglutination reaction for the detection of the lassa virus]. | 1988 | 3195147 | |
| immune serum increases arenavirus replication in monocytes. | the u937 monocytic cell line was used to determine whether antibodies could facilitate infection and replication of the arenaviruses, pichinde virus (pv) and lassa fever virus (lfv). when high dilutions of pv-immune serum were added to cultures simultaneously with pv inoculum, virus replication was dramatically (1000-fold) increased. low dilutions of this antiserum neutralized the virus. lfv also replicated in u937 cells. the presence of lfv-specific immune serum in the growth medium increased t ... | 1988 | 3292697 |
| expression of lassa virus nucleocapsid protein segments in bacteria: purification of high-level expression products and their application in antibody detection. | the lassa virus nucleocapsid protein gene and segments from it were expressed in escherichia coli under the control of the lac promoter in puc-based plasmids. expression of the near full-length protein [amino acid (aa) residues 12-570] fused to an n-terminal sequence of vector-derived 6 aa was not particularly efficient, and neither was that of a smaller n-terminal segment (aa 6-201) which was also fused at its c terminus to the remainder of the lacz gene product. by contrast, the c-terminal 370 ... | 1987 | 3315857 |
| lassa fever virus in senegal. | 1988 | 3343533 | |
| construction of a recombinant vaccinia virus expressing the lassa virus glycoprotein gene and protection of guinea pigs from a lethal lassa virus infection. | a cloned cdna (1.65 kb) containing the complete glycoprotein gene of the josiah strain of lassa virus was inserted into the thymidine kinase (tk) gene of the new york board of health (wyeth) strain of vaccinia virus. the lassa virus glycoprotein precursor, gpc, and the posttranslational cleavage products, g1 and g2, were shown by western blot analysis to be properly expressed in cells infected with the recombinant virus. northern blot hybridization of total cytoplasmic rna extracted from recombi ... | 1988 | 3354260 |
| [electron microscopic study of lassa virus]. | ultrafiltration through hollow fibrous filters followed by purification in interrupted and linear urografin gradients yielded a lassa virus suspension of high concentration. the use of gamma-irradiation for inactivation of the frozen virus suspension (-70 degrees c) caused no apparent structural changes of virions and made it possible to examine lassa virus in electron microscope by negative staining. the observed virus particles in their morphology and sizes did not differ from previously descr ... | 1988 | 3369149 |
| [clinico-epidemiologic and laboratory research on hemorrhagic fevers in guinea]. | in 1982-1983, were reported the cases of haemorrhagic fevers among populations living in the madina-ula district of guinea. clinico-epidemiological and serological studies (experimental studies) reveal into presumption of ebola and lassa fever viruses significance in the etiology of the disease outbreaks. antibodies to ebola virus were recognized in 19% from total number of sweating reconvalescent patients with the same clinical features, in order to 8% in healthy local populations. antibodies t ... | 1987 | 3440310 |
| use of monoclonal antibody for the detection of lassa virus antibody and antigen in patients with lassa fever. | 1986 | 3523183 | |
| expression of lassa virus nucleocapsid gene fragments in bacteria. | 1986 | 3523185 | |
| [indirect immunoenzyme method for the laboratory diagnosis of lassa and ebola hemorrhagic fevers]. | conditions for performing solid-phase indirect enzyme-immunoassay (speia) for the detection of lassa and ebola virus antigens and antibodies to them using horseradish peroxidase-labeled antispecific globulins were developed. the method is highly sensitive, specific, and reproducible. by this method, antigens of lassa and ebola viruses could be detected in tissue culture fluid of the infected cell cultures and in animal organ suspensions. detection of antibodies to lassa and ebola viruses in huma ... | 1986 | 3524001 |
| physiological and immunologic disturbances associated with shock in a primate model of lassa fever. | the degree of cell and organ damage in clinical and histological studies of patients dying of lassa fever has been insufficient to explain the catastrophic shock characteristic of the fatal illness. to explore this issue further, we conducted a study of the evolution of shock in three lassa virus-infected rhesus monkeys. by the sixth day after infection, a marked, progressive reduction of in vitro platelet aggregation occurred despite normal numbers of circulating platelets and a normal platelet ... | 1987 | 3543155 |
| standardization of a plaque assay for lassa virus. | the plaque reduction neutralization test (prnt) has been used routinely in serological studies with such arenaviruses as junin, machupo, and parana. however, difficulties have been encountered in using the prnt for lcm virus, while conflicting views have been expressed about the reliability and efficacy of the test with lassa virus. we have therefore investigated and evaluated the plaque assay for lassa virus. in addition, the suitability of the prnt for determining the potency of a serum and it ... | 1987 | 3585290 |
| [current serologic data on viral hemorrhagic fevers in the central african republic]. | during the years 1984-1985, 1,528 serum samples were taken through out the car. of these sera, 319 (20.8%) contained anti-filoviridae antibodies (ebola, marburg). this figure is higher than those found in cameroon, sudan, gabon and zaïre. three zones of the country are particularly exposed, the north-east where the population is in the contact with sudan ebola, the south-east where it is in contact with zaïre ebola, and the south-west where it is in contact with sudan ebola. the authors believe ... | 1987 | 3607998 |
| molecular structure and early events in the replication of tacaribe arenavirus s rna. | tacaribe arenavirus s rna was cloned and analysis of its nucleotide sequence revealed two open reading frames of significant size, one in the virus-sense strand, the other in the virus-complementary strand. the predicted amino acid sequences of the two reading frames were compared with the predicted primary structures of the nucleoprotein (n) and glycoprotein precursor (gpc) of lcm, pichinde and lassa viruses. the results indicated a high degree of homology between the proteins of similar proper ... | 1987 | 3617928 |
| clinical, epidemiologic, and therapeutic aspects of lassa fever. | 1986 | 3724661 | |
| evolutionary biology of a lassa virus complex. | 1986 | 3724662 | |
| serological and biological evidence that lassa-complex arenaviruses are widely distributed in africa. | 1986 | 3724663 | |
| serology and virulence diversity among old-world arenaviruses, and the relevance to vaccine development. | 1986 | 3724664 | |
| some biochemical properties of lassa virus rna and polypeptides. | 1986 | 3724668 | |
| nucleotide sequence of the glycoprotein gene and intergenic region of the lassa virus s genome rna. | two overlapping cdna clones corresponding to the 5' region of the lassa virus s genome rna were isolated and their nucleotide sequences determined. similar to pichinde and lymphocytic choriomeningitis viruses (lcmv), lassa virus has an ambisense s rna. the precursor to the viral glycoproteins (gpc) is encoded in viral rna sequence originating at position 56 and terminating at position 1529 from the 5' terminus of the s rna. a short, noncoding, intergenic region capable of forming a hairpin struc ... | 1986 | 3750844 |
| lassa fever--new issues raised by field studies in west africa. | 1987 | 3805770 | |
| a prospective study of the epidemiology and ecology of lassa fever. | in studies of lassa fever in sierra leone, the prevalence of human antibody to lassa virus ranged from 8% to 52%. mastomys natalensis, the reservoir of lassa virus, constituted 50%-60% of the rodents captured in houses but only 10%-20% of those captured in surrounding agriculture and bush areas (chi 2 = 90.2, p less than 10(-6), df = 1), a finding suggesting that houses are the most-important location for transmission of lassa virus. viral infection of mastomys from houses ranged from 0% to 80%. ... | 1987 | 3805771 |
| clinical virology of lassa fever in hospitalized patients. | we measured levels of virus in sequential specimens from 137 patients with lassa fever. the probability of fatal disease increased significantly with the level of viremia measured either on admission or during the course of illness. the odds ratio of death in patients with viremia greater than 10 tcid50/ml was 3.7 (90% confidence interval, 1.9-7.2). the same ratio in patients with viremia greater than 10 tcid50/ml and with levels of aspartate aminotransferase greater than or equal to 150 iu/lite ... | 1987 | 3805773 |
| lassa fever in children in sierra leone, west africa. | a systematic study of lassa fever in febrile children was undertaken over a four-year period, from august 1977 to august 1981, in the eastern province of sierra leone, west africa. 479 patients were studied; of these, 245 had adequate specimens to confirm or exclude the laboratory diagnosis of lassa fever. 51 cases of lassa fever were identified (21% of patients from whom diagnosis was possible). virus was isolated from 23 patients. all children had fever; cough and vomiting were present in over ... | 1986 | 3810792 |
| pediatric lassa fever: a review of 33 liberian cases. | thirty-three cases of pediatric lassa fever were identified at curran lutheran hospital and phebe hospital in liberia between january 1980 and march 1984. all 18 fetal cases died and the case-fatality rate for 15 childhood cases was 27%. we identified four clinical presentations according to age, including a case of congenital lassa fever, a condition not reported previously. two cases of lassa fever were found serologically during a one-month survey of all pediatric admissions at curran luthera ... | 1987 | 3826501 |
| molecular cloning of lassa virus rna: nucleotide sequence and expression of the nucleocapsid protein gene. | lassa virus rna isolated from purified virus particles was polyadenylated and reverse transcripts were cloned into the psti site of plasmid puc9. clones containing sequences of the smaller (s) segment of the lassa virus genome were identified by hybridization with purified s rna. they were characterized by their ability to hybridize with fragments of 3'-labeled lassa virus s rna and with each other, and by restriction mapping. the largest insert was 1830 bp long and began with the 3'-terminal 19 ... | 1985 | 3840620 |
| endemic lassa fever in liberia. iii. characterization of lassa virus isolates. | sixty-three virus isolates were obtained by inoculation of vero cells with sera from 50 hospital in-patients in liberia with acute febrile illnesses. 57 of the isolates were presumptively identified as lassa virus (lv) by direct fluorescent antibody (dfa) staining of inoculated vero cells. these, and six additional isolates obtained only by titration of supernatant fluids from inoculated vero cells, were definitively identified as lv in a neutralization test. two additional lv isolates were obta ... | 1985 | 3898483 |
| endemic lassa fever in liberia. iv. selection of optimally effective plasma for treatment by passive immunization. | the efficacy of passive immunization for treatment of lassa fever (lf) is believed to depend on the titre of the neutralizing antibody infused. for the purpose of identifying optimal donors of lv-immune plasma, a population of lf-convalescent patients in liberia was tested for prevalence of neutralizing antibody. minimally protective titres, expressed as a log10 neutralization index, (lni), were established in animal models as lni greater than 2. lni titres for 26 donors, tested eight or more mo ... | 1985 | 3898484 |
| [method for the simultaneous detection of immunofluorescing antibodies to the causative agents of different hemorrhagic fevers]. | 1985 | 3907144 | |
| lassa fever. effective therapy with ribavirin. | in a study of lassa fever in sierra leone, west africa, we identified two variables associated with a high risk of death, and we evaluated the efficacy of ribavirin and lassa virus-convalescent plasma for the treatment of lassa fever. a serum aspartate aminotransferase level greater than or equal to 150 iu per liter at the time of hospital admission was associated with a case-fatality rate of 55 percent (33 of 60). patients with the same risk factor who were treated for 10 days with intravenous ... | 1986 | 3940312 |
| lassa virus hepatitis: a study of fatal lassa fever in humans. | in order to explore the significance of a previous observation that the most important pathologic changes in fatal lassa fever are hepatic, we have studied postmortem liver biopsies from 19 patients with fatal lassa fever. we observed a vigorous macrophage response to cellular damage, but we found no evidence of lymphocyte infiltration in infected hepatic tissues. using semi-quantitative estimates of liver cell damage, we found a wide range in the severity and progression of lassa virus hepatiti ... | 1986 | 3953952 |
| kinetic study of platelets and fibrinogen in lassa virus-infected monkeys and early pathologic events in mopeia virus-infected monkeys. | the rhesus monkey, an established model of lassa fever, was used to study hematologic and hemostatic aspects of lassa fever and whether mopeia (also known as mozambique) virus induces any cellular damage in this model. six days after subcutaneous injection of 10(3.48) plaque forming units (pfu) of lassa virus (josiah strain) one group of monkeys received an intravenous injection of 111in-labeled allogeneic platelets and another group received 125i-labeled alogeneic fibrinogen. lassa virus-infect ... | 1985 | 4037187 |
| lassa virus lethality for inbred mice. | 1985 | 4037900 | |
| [experimental evaluation of the pathogenicity of lassa virus variants]. | pathogenicity for guinea pigs and white mice of various lassa virus variants: native, having had 1 passage in vero cell culture and 4 passages in newborn white mouse brain; a virus having gone through 10 passages in vero cells and 8 mouse brain passages (variant no. 10); a small-plaque clone derived from variant no. 10 by the method of plaque-to-plaque cloning (variant no. 11k), was studied. both the native virus and variant no. 10 were found to be similarly pathogenic for susceptible laboratory ... | 1985 | 4060702 |
| [pathogenicity of the lassa virus for laboratory mice]. | pathogenicity for randombred and inbred mice of various age groups of the standard lassa virus and the virus enriched with defective interfering particles (dip) was studied. the standard lassa virus inoculated intracerebrally caused 100% death of c3h/sn mice aged up to 4 weeks and 60%-70% death of randombred white mice aged 3-4 weeks. balb/c mice were found to be nonsusceptible to the virus, and its lethality for c57bl/6 and akr mice varied within the range of 30%-60%. lassa virus enriched with ... | 1985 | 4072162 |
| effect of beta-propiolactone on routine biochemical investigations. | 1985 | 4079681 | |
| editorial: rodent control and lassa fever. | 1974 | 4137628 | |
| lassa fever and marburg virus disease. | 1974 | 4208764 | |
| lassa fever. | 1974 | 4213599 | |
| [transport of a patient with lassa fever in a boeing 707 b]. | 1974 | 4281903 | |
| [african zoonoses caused by viruses]. | 1973 | 4346544 | |
| virus diseases associated with cutaneous eruptions. | 1973 | 4356897 | |
| [lassa virus disease]. | 1974 | 4407885 | |
| [lassa fever]. | 1974 | 4424106 | |
| lassa fever in the eastern province of sierra leone, 1970-1972. i. epidemiologic studies. | 1974 | 4429182 | |
| lassa fever in the eastern province of sierra leone, 1970-1972. ii. clinical observations and virological studies on selected hospital cases. | 1974 | 4429183 | |
| [lassa fever (author's transl)]. | 1974 | 4431978 | |
| [lassa-fever a new dangerous virus infection]. | 1974 | 4453164 | |
| undiagnosed fever--rickettsial, viral, and helminth infections. | 1974 | 4479614 | |
| [lassa fever (review of the literature)]. | 1973 | 4594659 | |
| hoosiers abroad--report of a case of lassa fever. | 1974 | 4604539 | |
| biochemical and biophysical properties of the arenaviruses. | 1974 | 4608220 | |
| tropical pathology. | 1974 | 4619032 | |
| [histopathological diagnosis of hepatitis due to lassa virus]. | 1972 | 4679205 | |
| editorial: tropical viral infections. | 1973 | 4745118 | |
| lassa fever. | 1973 | 4745120 | |
| a hospital epidemic of lassa fever in zorzor, liberia, march-april 1972. | 1973 | 4745236 | |
| clinical presentation of lassa fever cases during the hospital epidemic at zorzor, liberia, march-april 1972. | 1973 | 4745237 | |
| lassa fever in britain: an imported case. | 1973 | 4755184 | |
| lassa virus isolation from mastomys natalensis rodents during an epidemic in sierra leone. | lassa fever is a severe febrile illness of man, first recognized in west africa in 1969. during an epidemic in sierra leone, lassa virus was isolated for the first time from wild rodents of mastomys natalensis. a high prevalence of infected mastomys was found in houses occupied by patients with lassa fever. the data presented provide the first demonstration of an extra-human cycle of lassa virus transmission and suggest that rodent control may be an effective method of limiting the disease. | 1974 | 4833828 |
| lassa fever, a new virus disease of man from west africa. iv. electron microscopy of vero cell cultures infected with lassa virus. | 1970 | 4987548 | |
| [lassa virus]. | 1970 | 4991418 | |
| lassa fever. | 1972 | 5083882 | |
| isolation and antigenic characterization of lassa virus. | 1970 | 5428406 |