Publications

genome-wide analysis of synonymous codon usage in huaiyangshan virus and other bunyaviruses.	huaiyangshan virus (hysv) is a newly discovered bunyavirus, which is transmitted by ticks and causes hemorrhagic fever-like illness in human. the interplay of codon usage among viruses and their hosts is expected to affect viral survival, evasion from host's immune system and evolution. however, little is known about the codon usage in hysv genome. in the present study, we analyzed synonymous codon usage in 120 available full-length hysv sequences and performed a comparative analysis of synonymo ...	2015	26173646
low ph and anionic lipid-dependent fusion of uukuniemi phlebovirus to liposomes.	many phleboviruses (family bunyaviridae) are emerging as medically important viruses. these viruses enter target cells by endocytosis and low ph-dependent membrane fusion in late endosomes. however, the necessary and sufficient factors for fusion have not been fully characterized. we have studied the minimal fusion requirements of a prototypic phlebovirus, uukuniemi virus, in an in vitro virus-liposome assay. we show that efficient lipid mixing between viral and liposome membranes requires close ...	2016	26811337
differential use of the c-type lectins l-sign and dc-sign for phlebovirus endocytosis.	bunyaviruses represent a growing threat to humans and livestock globally. the receptors, cellular factors and endocytic pathways used by these emerging pathogens to infect cells remain largely unidentified and poorly characterized. dc-sign is a c-type lectin highly expressed on dermal dendritic cells that has been found to act as an authentic entry receptor for many phleboviruses (bunyaviridae), including rift valley fever virus (rvfv), toscana virus (tosv) and uukuniemi virus (uukv). we found t ...	2016	26990254
uukuniemi virus as a tick-borne virus model.	in the last decade, novel tick-borne pathogenic phleboviruses in the family bunyaviridae, all closely related to uukuniemi virus (uukv), have emerged on different continents. to reproduce the tick-mammal switch in vitro, we first established a reverse genetics system to rescue uukv with a genome close to that of the authentic virus isolated from the ixodes ricinus tick reservoir. the ire/ctvm19 and ire/ctvm20 cell lines, both derived from i. ricinus, were susceptible to the virus rescued from pl ...	2016	27194760
rna encapsidation and packaging in the phleboviruses.	the bunyaviridae represents the largest family of segmented rna viruses, which infect a staggering diversity of plants, animals, and insects. within the family bunyaviridae, the phlebovirus genus includes several important human and animal pathogens, including rift valley fever virus (rvfv), severe fever with thrombocytopenia syndrome virus (sftsv), uukuniemi virus (uukv), and the sandfly fever viruses. the phleboviruses have small tripartite rna genomes that encode a repertoire of 5-7 proteins. ...	2016	27428993
the role of phlebovirus glycoproteins in viral entry, assembly and release.	bunyaviruses are enveloped viruses with a tripartite rna genome that can pose a serious threat to animal and human health. members of the phlebovirus genus of the family bunyaviridae are transmitted by mosquitos and ticks to humans and include highly pathogenic agents like rift valley fever virus (rvfv) and severe fever with thrombocytopenia syndrome virus (sftsv) as well as viruses that do not cause disease in humans, like uukuniemi virus (uukv). phleboviruses and other bunyaviruses use their e ...	2016	27455305
differential antagonism of human innate immune responses by tick-borne phlebovirus nonstructural proteins.	in recent years, several newly discovered tick-borne viruses causing a wide spectrum of diseases in humans have been ascribed to the phlebovirus genus of the bunyaviridae family. the nonstructural protein (nss) of bunyaviruses is the main virulence factor and interferon (ifn) antagonist. we studied the molecular mechanisms of ifn antagonism employed by the nss proteins of human apathogenic uukuniemi virus (uukv) and those of heartland virus (hrtv) and severe fever with thrombocytopenia syndrome ...	2017	28680969