Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| molecular characterization of phocine distemper virus: gene order and sequence of the gene encoding the attachment (h) protein. | generation of a set of cdna clones covering the n, p/v/c, m, f, h and part of the l gene of phocine distemper virus (pdv) has been described. the gene order of pdv determined from a physical as well as a transcriptional map, was identical to that of the other morbilliviruses so far studied. the h gene sequence (1951 nucleotides) contains one large open reading frame which encodes a protein of 607 amino acids, identical in length to that of the h protein of the convac strain of canine distemper v ... | 1992 | 1588321 |
| molecular and serological studies on the recent seal virus epizootics in europe and siberia. | the virus epizootics which occurred in seals in both europe and siberia during 1987/1988 were caused by two different morbilliviruses, referred to as phocid distemper virus (pdv) 1 and 2, respectively. molecular and serological studies have shown that the european virus is quite distinct from canine distemper virus (cdv), its closest relative in the morbillivirus group. analysis of tissues obtained from infected seals from a wide geographical distribution over northern europe showed that the inf ... | 1992 | 1594928 |
| phocine distemper virus, the agent responsible for the 1988 mass mortality of seals. | the biochemical characterisation of phocine distemper virus (pdv) has shown that pdv is related to but clearly distinct from canine distemper virus (cdv) and relative to its relationship with cdv is only remotely related to the other morbilliviruses, namely measles virus (mv) or rinderpest virus (rpv) and peste-des-petits-ruminants virus (pprv). comparative studies with monoclonal antibodies indicate that the virus is serologically closely related to cdv with many conserved epitopes, particularl ... | 1992 | 1594934 |
| the serological response of the common seal (phoca vitulina) and the grey seal (halichoerus grypus) to phocine distemper virus as measured by a canine distemper virus neutralisation test. | the application of a canine distemper virus (cdv) neutralisation test revealed that antibody titres of up to 11,620 were present in the sera of common seals during the epizootic of phocine distemper in british waters. however, during the later stages of the epizootic and afterwards, a surprisingly high proportion of animals were still seronegative. titres of from 90 to 183 were common in diseased animals and were probably indicative of partial immunosuppression. a few animals with proven phocine ... | 1992 | 1594940 |
| canine distemper infection associated with acute nervous signs in dogs. | eight vaccinated dogs suddenly developed progressive ataxia, paresis or paralysis of short duration. a histopathological examination revealed a non-suppurative meningoencephalitis suggestive of a viral infection, and immunohistochemical examination confirmed the presence of canine distemper virus antigen in five of the dogs. distemper had not been suspected from the clinical examination of the dogs. | 1992 | 1595146 |
| the nucleotide and deduced amino acid sequence of the m gene of phocid distemper virus (pdv). the most conserved protein of morbilliviruses shows a uniquely close relationship between pdv and canine distemper virus. | the nucleotide sequence of the matrix gene (m) of a recently identified morbillivirus, phocid distemper virus (pdv), was determined and the amino acid composition deduced. the m gene of pdv shared many characteristics with the corresponding gene in other morbilliviruses. the nucleotide homology with the closely related canine distemper virus (cdv) was maximum at 67% followed by measles virus (mv) (58%) and rinderpest virus (rpv) (56%). the length of the 5' long untranslated region of pdv (408) w ... | 1992 | 1604930 |
| canine distemper virus iscoms induce protection in harbour seals (phoca vitulina) against phocid distemper but still allow subsequent infection with phocid distemper virus-1. | a candidate canine distemper virus (cdv) iscom vaccine has been shown to be effective in protecting harbour seals (phoca vitulina) from phocid distemper in 1988. however, of the 35 harbour seals receiving this vaccine upon admission to a seal rehabilitation and research centre (pieterburen, the netherlands) in 1989, six developed mild inflammatory symptoms of the respiratory tract. phocid distemper virus-1 (pdv-1) could be isolated from three of these animals. this indicates that the vaccine aff ... | 1992 | 1609546 |
| anti-canine distemper virus antibodies in common and grey seals. | 1992 | 1621345 | |
| sequence analysis of the genes encoding the nucleocapsid protein and phosphoprotein (p) of phocid distemper virus, and editing of the p gene transcript. | the nucleotide and deduced amino acid sequences of two genes of phocid distemper virus (pdv) were determined by cdna cloning and sequencing. the long open reading frame of the gene encoding the nucleocapsid (n) protein is presented. as with other morbilliviruses, the phosphoprotein (p) gene of pdv was found to be located after the 5' end of the n gene and before the 3' end of the matrix protein gene. the p gene was shown to have the capacity to encode three distinct proteins, p, v and c, in anal ... | 1992 | 1634877 |
| detection of canine distemper virus in bone cells in the metaphyses of distemper-infected dogs. | in the light of recent evidence implicating canine distemper virus (cdv) as a possible etiologic agent in paget's disease of bone, we thought that it would be of interest to examine distemper-infected bone in the natural host. samples from the long bones, spleen, and bladder of four distemper-infected and three uninfected dogs were examined for the presence of cdv nucleocapsid and phosphoprotein genes and the measles virus (mv) nucleocapsid gene using the technique of in situ hybridization with ... | 1992 | 1642150 |
| characteristics of a cetacean morbillivirus isolated from a porpoise (phocoena phocoena). | a virus isolated from a porpoise during the 1988 seal epizootic was shown to be a morbillivirus. in order to determine the relationship of the virus to phocine distemper virus (pdv) a battery of monoclonal antibodies raised against canine distemper virus (cdv), pdv or the porpoise isolate were assessed for their ability to bind to cdv, pdv or porpoise virus epitopes in indirect immunofluorescence assays and elisas. the porpoise isolate contained several unique epitopes and several epitopes prese ... | 1992 | 1642556 |
| prevalence of antibodies against canine parvovirus and canine distemper virus in wild coyotes in southeastern colorado. | serum from 72 wild coyotes (canis latrans) in southeastern colorado (usa) was collected and analyzed for prevalence of antibody to canine parvovirus (cpv) and canine distemper virus (cdv) from 1985 to 1988. the prevalence of antibodies to cpv and cdv was 71% and 57%, respectively, for the 4 yr of the study. prevalence of antibody to cpv did not differ among years, between sexes, or with age. prevalence of antibody to cdv did not differ among years or between sexes, but was significantly higher i ... | 1991 | 1648628 |
| vaccination of dogs with multi-component vaccines. | 1991 | 1652929 | |
| immunological relationships between phocid and canine distemper virus studied with monoclonal antibodies. | the immunological relationships between distemper viruses, isolated from a seal and mink in denmark and from a dog in greenland, were investigated with 39 previously developed monoclonal antibodies (mabs) directed against four major structural proteins of canine distemper virus (cdv). they were also investigated with 16 newly developed mabs directed against the fusion (f) and large glycoprotein (named h in analogy with measles virus) of phocid distemper virus (pdv) isolated from a harbour seal ( ... | 1990 | 1698923 |
| detection of phocid distemper virus rna in seal tissues using slot hybridization and the polymerase chain reaction amplification assay: genetic evidence that the virus is distinct from canine distemper virus. | slot hybridization and the polymerase chain reaction (pcr) after reverse transcription (rt) were used to detect rna extracted from tissues of seals after naturally occurring disease and experimental infection with phocid distemper virus (pdv). a phosphoprotein (p) gene-specific cdna served as a probe for both slot hybridization and the identification of pcr-generated fragments by southern blotting. as primers for the pcr assay pdv p gene-derived oligonucleotides were used. hybridization, pcr and ... | 1991 | 1707946 |
| identification of epitope(s) on the internal virion proteins of rinderpest virus which are absent from peste des petits ruminants virus. | monoclonal antibodies (mab) raised against the rbok vaccine strain of rinderpest virus were characterized by radio-immunoprecipitation (ripa) and in the indirect elisa using measles (mv), distemper (cdv), rinderpest (rpv) and peste des petits ruminants viruses (pprv). those found to be specific for the matrix (m) protein and the nucleocapsid (n) protein could be classified into different groups on the basis of the anti-morbillivirus mab classification scheme; a number of these mab showed a selec ... | 1991 | 1709543 |
| antigenic and functional characterization of rinderpest virus envelope proteins using monoclonal antibodies. | a total of 24 monoclonal antibodies (mabs) against the haemagglutinin (h) and the fusion protein (f) of rinderpest virus (rpv) were used to characterize their antigenic structure and biological properties, and to analyse natural variation in the envelope proteins of morbilliviruses. the anti-h and anti-f mabs defined seven and three distinct antigenic sites, respectively. the mabs to six sites on h were able to neutralize the infectivity of rpv. the addition of guinea-pig complement or anti-mous ... | 1991 | 1714940 |
| [the persistence of the distemper virus in continuous human cells]. | two cultures chronically infected with distemper virus (hep-2 and l-41) were obtained. the cultures produced a small-plaque cell-associated virus and a virus-specific antigen which was demonstrated by the fluorescence antibody technique in 40%-60% of the cells. the chronically infected cells produced interferon as judged by their resistance to superinfections with heterologous viruses. the virus-carrier state was characterized by temperature sensitivity. | 1991 | 1715629 |
| loss of virulence of canine distemper virus is associated with a structural change recognized by a monoclonal antibody. | the monoclonal antibody (mab) l1, which binds to the nucleocapsid protein of canine distemper virus (cdv), was shown to bind to avirulent cdv obtained after serial passages in vero cells, but not to two different virulent demyelinating cdv-strains propagated in dog glial cell cultures. however, when both virulent cdv-strains were passaged through vero cells they expressed, after a number of passages, an epitope recognized by mab l1. the occurrence of the l1 epitope appeared to coincide with loss ... | 1991 | 1717309 |
| protective effects of monoclonal antibodies against lethal canine distemper virus infection in mice. | monoclonal antibodies (mabs) against the haemagglutinin (h), fusion protein (f) and nucleoprotein of canine distemper virus (cdv) were examined for their ability to protect mice against lethal cdv infection. one mab against h and two of six mabs against f protected mice, the protective effect of the anti-h mab being stronger than that of the anti-f mabs. the anti-h mab showed virus neutralizing activity, but the two anti-f mabs, which recognized the same epitope, did not. protection by the anti- ... | 1991 | 1719129 |
| phocine distemper virus is phylogenetically related to canine distemper virus. | 1991 | 1720585 | |
| nonreplicating viral vectors as potential vaccines: recombinant canarypox virus expressing measles virus fusion (f) and hemagglutinin (ha) glycoproteins. | the development of canarypox virus (cpv) recombinants expressing the hemagglutinin (ha) and fusion (f) glycoproteins of measles virus (mv) is described. inoculation of the cpv-mv recombinants into avian or nonavian tissue culture substrates led to the expression of authentic mvf and mvha as determined by radioimmunoprecipitation and surface immunofluorescence. in contrast to avian-derived tissue culture, no productive replication of the cpv recombinant was evident in tissue culture cells derived ... | 1992 | 1736535 |
| virus-induced obesity in mice: association with a hypothalamic lesion. | in an earlier study we found that a substantial percentage of mice surviving infection with canine distemper virus (cdv) slowly developed a morbid obesity syndrome. in the present study we wished to explore the role of the virus in the development of this syndrome. the distribution of viral antigen(s) in brains of pre-obese animals shortly after intracerebral infection was mapped using immunocytochemical procedures. a distinctive pattern of cell labeling was found, extending from the anterior pe ... | 1992 | 1740670 |
| a sarcocystis sp.-like protozoan and concurrent canine distemper virus infection associated with encephalitis in a raccoon (procyon lotor). | a raccoon (procyon lotor) with signs of weakness was captured in upstate new york (usa). despite attempted care in a rehabilitation facility, the animal died and was examined because of suspected infectious neurologic disease. the cerebrum had a marked, locally extensive, neutrophilic, necrotizing encephalitis with numerous associated intralesional protozoal organisms, and a moderate to marked multifocal perivascular nonsuppurative meningoencephalitis. based on morphology and immunohistochemical ... | 1991 | 1758036 |
| attempt to vaccinate orally harbour seals against phocid distemper. | 1991 | 1759340 | |
| correlation between humoral immune responses and presence of virus in the cns in dogs experimentally infected with canine distemper virus. | the role of the humoral immune response in clearance or prevention of canine distemper viral encephalitis of dogs infected with a virulent strain of canine distemper virus has been evaluated. dogs that have demyelinating lesions, cdv proteins and infectious virus in their brains demonstrate an impaired humoral immune response. in dogs that recover from infection and contain no demyelinating lesions, viral proteins or infectious virus in the brain, antibodies to the internal proteins of cdv are o ... | 1991 | 1759903 |
| the nucleotide sequence and deduced amino acid composition of the haemagglutinin and fusion proteins of the morbillivirus phocid distemper virus. | the amino acid composition of the two surface proteins of the recently isolated morbillivirus phocid distemper virus (pdv) were deduced from the nucleotide sequence. the fusion (f) protein of pdv exhibited characteristics similar to those of other morbillivirus f proteins. the overall amino acid similarity with its closest homologue, canine distemper virus (cdv), was 72%. from the context of the starting codons and the requirement for a hydrophobic signal peptide, it is likely that translation o ... | 1991 | 1765768 |
| [demonstration of viral proteins and rna in hypothalamus of mice infected by canine distemper virus]. | there are a number of reports suggesting that neurological disorders may be due to infectious agents, such as viruses. in order to study the role of viruses on cellular plasticity in the central nervous system, we established a model of virus infection in the mouse. inoculation of mouse with canine distemper virus (cdv) led to an acute encephalitis, late neurological disorders and an obesity syndrome. to analyse the role of viral replication on the development of this syndrome we studied the cer ... | 1991 | 1773359 |
| the seal death in danish waters 1988. 2. virological studies. | mass abortions and high mortality were observed in harbour seals in danish waters during 1988. severe pneumonia and emphysema were typical clinical and post-mortem findings. virological studies were carried out to identify the cause of the epidemic. although seal herpesvirus (sehv) was isolated in 23 of 114 animals this virus was subsequently found not to be the primary cause of the disease. following the observation of seroconversion against canine distemper virus (cdv) in diseased seals (oster ... | 1991 | 1803934 |
| failure to detect paramyxovirus sequences in paget's disease of bone using the polymerase chain reaction. | it is widely believed that paget's disease of bone is due to a "slow virus" infection of osteoclasts with one of the paramyxovirus group. controversy continues to surround the identity of the virus involved, however, since at different times evidence has been presented implicating measles virus (mv), respiratory syncytial virus (rsv), and canine distemper virus (cdv) as putative infective agents. in this study we used the technique of reverse transcription and polymerase chain reaction (pcr) to ... | 1991 | 1805546 |
| vaccinia virus recombinants expressing either the measles virus fusion or hemagglutinin glycoprotein protect dogs against canine distemper virus challenge. | cdna clones of the genes encoding either the hemagglutinin (ha) or fusion (f) proteins of the edmonston strain of measles virus (mv) were expressed in vaccinia virus recombinants. immunofluorescence analysis detected both proteins on the plasma membranes of unfixed cells as well as internally in fixed cells. immunoprecipitation of metabolically radiolabeled infected-cell extracts by using specific sera demonstrated a 76-kda ha polypeptide and gene products of 60, 44, and 23 kda which correspond ... | 1991 | 1830113 |
| virulent and attenuated canine distemper virus infects multiple dog brain cell types in vitro. | canine distemper virus (cdv) produces an encephalitis in dogs that varies with viral strain. we have studied the cell tropisms of two virulent strains (cdv-sh and cdv a75-17) and an attenuated strain, rockborn (cdv-ro), in cultured canine brain cells. infected cell types were identified by double immunofluorescent labeling of specific cell markers and viral antigens. all viral strains studied produced infection in astrocytes, fibroblasts, and macrophages. neurons were not infected by cdv a75-17 ... | 1991 | 1834561 |
| canine distemper virus infection and encephalitis in javelinas (collared peccaries). | canine distemper virus has been isolated in dog lymphocyte cultures from the brains of three javelinas that became moribund with signs of encephalitis. canine distemper viral antigen was demonstrated predominantly in neurons and morbillivirus-like structures were seen by electron microscopy in brains of diseased animals. serological studies suggest that cdv infection may be common in javelinas. | 1991 | 1863221 |
| the nucleotide and predicted amino acid sequence of the attachment protein of canine distemper virus. | the nucleotide sequence of the gene coding for the attachment protein of the convac strain of the canine distemper virus (cdv), corresponding to the haemagglutinin (h) gene of measles virus was determined using a mrna-derived cdna clone and genomic viral rna. the mrna transcribed from the cdv h gene is 1944 nucleotides long excluding the polyadenylated tail. only one long open reading frame was found comprising nucleotides 21-1841. the predicted protein has a single hydrophobic region which can ... | 1991 | 1891961 |
| comparison of in vitro replication and cytopathology caused by strains of canine distemper virus of vaccine and field origin. | three biological properties of canine distemper virus were examined to determine if any would consistently differentiate field from vaccine strains of the virus. the properties were the ability to (1) infect macrophages and epithelial cells, (2) produce distinct cytopathologic effect in alveolar and peritoneal macrophages and vero cells, and (3) produce pocks on the chorioallantoic membrane of embryonated chicken eggs. four vaccine strains and 5 field isolates were used in the study. the 5 field ... | 1991 | 1892930 |
| canine distemper virus localised in bone cells of patients with paget's disease. | the technique of in situ hybridisation was applied using radioactively labelled riboprobes to examine for the presence of canine distemper (cdv) and measles (mv) rna in paget's disease of bone. the results indicate that in 41% of paget's patients, cdv rna could be detected in osteoclasts, osteoblasts, and osteocytes, but not in controls. in contrast, rna to the measles virus was not detected. we suggest that cdv may in some cases play a role in the aetiology of paget's disease. | 1991 | 1910961 |
| relationship between rheumatological diseases of man, pet dogs and their viruses. | 1991 | 1913019 | |
| where next for canine virus? | 1991 | 1922358 | |
| selective degeneration of oligodendrocytes mediated by reactive oxygen species. | the mechanism underlying demyelination in inflammatory canine distemper encephalitis is uncertain. macrophages and their secretory products are thought to play an important effector role in this lesion. recently, we have shown that anti-canine distemper virus antibodies, known to occur in chronic inflammatory lesions, stimulate macrophages leading to the secretion of reactive oxygen species (ros). to investigate whether ros could be involved in demyelination, dog glial cell cultures were exposed ... | 1990 | 1965721 |
| the nucleotide sequence of the gene encoding the attachment protein h of canine distemper virus. | the sequence of the h gene and flanking sequences in the f and l genes of canine distemper virus (cdv) have been determined. the h gene of cdv (1946 nucleotides) contains one large open reading frame starting at position 21 and terminating at position 1835, encoding a protein of 604 amino acid residues. this protein contains three potential glycosylation sites in the extracellular domain and, like all other paramyxoviruses, a n-terminal membrane-spanning hydrophobic anchor domain. the deduced h ... | 1991 | 1993883 |
| nucleotide sequence of cdna to the rinderpest virus mrna encoding the nucleocapsid protein. | the full-length cdna corresponding to the mrna encoding the nucleocapsid protein (np) of rinderpest virus (rv) was cloned and its complete nucleotide sequence was determined. the gene of rv-np was composed of 1683 nucleotides and contained a single large open reading frame, which is capable of encoding a protein of 525 amino acids with a molecular weight of 58,241 da. the nucleotide sequence and predicted amino acid sequence were compared with those of measles virus (mv) and canine distemper vir ... | 1991 | 2017875 |
| viral expression in experimental canine distemper demyelinating encephalitis. | we have characterized the relationship between the expression of canine distemper virus (cdv) and demyelinating lesions in the white matter of the cerebellum of experimentally infected dogs. in animals which had demyelinating lesions, cdv proteins (n, p, f and h) were expressed and infectious virus could be recovered from brain tissue. viral proteins (n, p, f and h) were detected by monoclonal antibodies and immunocytochemistry within demyelinating lesions as well as in scattered glial cells in ... | 1991 | 2019678 |
| detection of igm antibodies against canine distemper virus in dog and mink sera employing enzyme-linked immunosorbent assay (elisa). | an enzyme-linked immunosorbent assay (elisa) for the detection of igm antibodies against canine distemper virus (cdv) in canine and mink serum is described. the diagnostic potential of this technique was evaluated by analyzing sera from natural or experimental infections in dog and mink and negative control sera. these results were compared with results obtained in the developed cdv igg elisa and in the virus neutralization test. the igm test, which requires only a single serum specimen, is a us ... | 1991 | 2039785 |
| canine distemper viral antigens and antibodies in dogs with rheumatoid arthritis. | dogs with canine rheumatoid arthritis had significantly elevated levels of antibodies to canine distemper virus. this increase was particularly seen in the synovial fluids, compared with paired sera, and was not found in dogs with infective arthropathies, osteoarthritis or in osteoarthritis secondary to rupture of the cranial cruciate ligament. analysis of the immune complexes precipitated from synovial fluids showed immunoglobulins in all types of arthropathy. western blotting analyses showed r ... | 1991 | 2047594 |
| isolation and characterisation of a porpoise morbillivirus. | a morbillivirus was isolated from lung tissue of a porpoise which had lesions similar to those of phocine distemper. porpoise and seal isolates differed in their reactions to monoclonal antibodies to canine distemper virus. | 1991 | 2069505 |
| analysis of antigenic differences between sixteen phocine distemper virus isolates and other morbilliviruses. | monoclonal antibodies against a phocine morbillivirus isolate (pdv 2558/han 88) were able to discriminate sixteen pdv isolates from any other morbillivirus species providing further evidence that pdv should be regarded a new species in the morbillivirus genus. | 1991 | 2069507 |
| comparison of two morbilliviruses isolated from seals during outbreaks of distemper in north west europe and siberia. | recently morbilliviruses were isolated from harbour seals (phoca vitulina) in north west europe (phocid distemper virus-1: pdv-1) and from baikal seals (phoca sibirica) in siberia (phocid distemper virus-2: pdv-2) during outbreaks of severe disease which resembled distemper in dogs. pdv-1 and pdv-2 were passaged in spf dogs, in which they caused distemper-like disease symptoms, and were subsequently passaged in vero cells in which they caused cytopathic changes. pdv-1, pdv-2, and canine distempe ... | 1990 | 2141248 |
| sequencing analyses and comparison of parainfluenza virus type 4a and 4b np protein genes. | the nucleotide sequences of the cdna copies of the mrna coding for the nucleocapsid proteins (nps) of human parainfluenza viruses type 4a (piv-4a) and type 4b (piv-4b) were determined. the copy of piv-4a np mrna contained 1885 nucleotides encoding a protein with a calculated molecular weight of 62,561. the same number of amino acids with a similar molecular weight (62,425) were predicted for the piv-4b np protein. comparisons on the nucleotide sequence and the amino acid sequence of np protein b ... | 1990 | 2152988 |
| occurrence of a canine distemper-like disease in aquarium seals. | outbreaks of a canine distemper-like acute disease brought high mortalities to seal populations in north-west europe and lake baikal from late 1987 to 1988. during these outbreaks three seals which were introduced from lake baikal to an aquarium in japan developed a distemper-like disease and other seals raised in the same room were similarly affected. clinical signs of dead seals were anorexia, diphasic fever, dyspnea, and neuromuscular tics. characteristic microscopic lesions of acute intersti ... | 1990 | 2166849 |
| sequence analysis of the phosphoprotein (p) genes of human parainfluenza type 4a and 4b viruses and rna editing at transcript of the p genes: the number of g residues added is imprecise. | we cloned and sequenced the cdnas against genomic rnas and mrnas for phosphoproteins (ps) of human parainfluenza virus types 4a (piv-4a) and 4b (piv-4b). the piv-4a and -4b p genes were 1535 nucleotides including poly(a) tract and were found to have two small open reading frames, neither of which was apparently large enough to encode the p protein. a cluster of g residues was found in genomic rna and the number of g residues was 6 in both piv-4a and -4b. however, the number of g residues at the ... | 1990 | 2167560 |
| sequence analyses of the 3' genome end and np gene of human parainfluenza type 2 virus: sequence variation of the gene-starting signal and the conserved 3' end. | we cloned and determined the nucleotide sequences of cdnas against nucleocapsid protein (np) mrna and the genomic rna of human parainfluenza type 2 virus (piv-2). the 3' terminal region of genomic rna was compared among piv-2, mumps virus (muv), newcastle disease virus (ndv), measles virus (mv), piv-3, bovine parainfluenza type 3 virus (bpiv-3), sendai virus (sv), and vesicular stomatitis virus (vsv), and an extensive sequence homology was observed between piv-2 and muv. although no significant ... | 1990 | 2173261 |
| complete nucleotide sequence of the matrix gene of human parainfluenza type 2 virus and expression of the m protein in bacteria. | the sequence of the m gene of human parainfluenza virus type 2 (piv-2) has been determined. the sequence contained a large open reading frame with 1131 nucleotides encoding a protein with a calculated molecular weight of 42,312. comparison of m protein sequence indicated that piv-2 was more closely related to mumps virus and newcastle disease virus than to other parainfluenza viruses, sendai virus (sv), and parainfluenza virus type 3 (piv-3), indicating a possible subdividing of the paramyxoviru ... | 1990 | 2173264 |
| detection of canine distemper viral antigen in formalin-fixed and paraffin-embedded tissue of a fitch (mustela putorius), using an immunoperoxidase technique. | the present study describes how a naturally infected fitch (mustela putorius) caused an outbreak of canine distemper in a colony of insufficiently vaccinated dogs. the detection of canine distemper virus (cdv) on paraffin sections of different organs of the fitch and one of the dead dogs was achieved using a monoclonal antibody against the nucleocapsid protein (np) of cdv and the avidin-biotin-peroxidase complex (abc) technique. | 1990 | 2178907 |
| [distemper as the cause of death in badgers in austria]. | a canine distemper virus infection of badgers in a hunting range in austria is described. a badger which was shot after showing symptoms of rabies infection and one which was found dead were examined by gross pathology and parasitological, histological, bacteriological and virological methods. the examination for rabies was negative in both cases. the badger which was found dead histologically showed signs of a non purulent panencephalitis, the shot animal showed hyperaemia and oedema of the bra ... | 1990 | 2183393 |
| seal distemper in norway in 1988 and 1989. | 1990 | 2192495 | |
| mass mortality in seals caused by a newly discovered morbillivirus. | during a recent disease outbreak among harbour seals (phoca vitulina) in the north and baltic seas, more than 17,000 animals have died. the clinical symptoms and pathological findings were similar to those of distemper in dogs. based on a seroepizootiological study, using a canine distemper virus (cdv) neutralization assay, it was shown that cdv or a closely related morbillivirus (phocid distemper virus-pdv) was the primary cause of the disease. the virus was isolated in cell culture from the or ... | 1990 | 2205971 |
| immunohistochemical demonstration of canine distemper virus antigen as an aid in the diagnosis of canine distemper encephalomyelitis. | brain tissue from 33 dogs with non-suppurative encephalitis was examined for evidence of canine distemper virus (cdv) encephalitis. sections were examined for lesions, inclusion bodies, syncytial cells and cdv antigen using a double bridge unlabelled antibody enzyme technique. histopathological lesions considered to be typical of granulomatous meningoencephalomyelitis were found in seven dogs. they all lacked inclusion bodies, syncytial cells and cdv antigen. the remaining 26 dogs all had histop ... | 1990 | 2236913 |
| phocid distemper virus--a threat to terrestrial mammals? | 1990 | 2238399 | |
| comparison of rinderpest and peste des petits ruminants viruses using anti-nucleoprotein monoclonal antibodies. | monoclonal antibodies (mabs) were obtained using a purified preparation of the rbok strain of a rinderpest vaccine virus. the cytoplasmic immunofluorescent staining test showed that these clones had specificity for the nucleoprotein (n) of the virus. six clones which immunoprecipitated the n protein corroborated these results. thirteen anti-n mabs were used to compare geographically widespread rinderpest viruses (rpv) and peste des petits ruminants viruses (pprv) to two other morbilliviruses, me ... | 1990 | 2247932 |
| [distemper in a population of the baikal seal]. | 1990 | 2256402 | |
| nucleotide sequence analysis of phocine distemper virus reveals its distinctness from canine distemper virus. | 1990 | 2264246 | |
| morbillivirus infections of seals during the 1988 epidemic in the bay of heligoland: iii. transmission studies of cell culture-propagated phocine distemper virus in harbour seals (phoca vitulina) and a grey seal (halichoerus grypus): clinical, virological and serological results. | eight harbour seals (phoca vitulina), two of them seronegative, six seropositive against pdv and a seronegative grey seal (halichoerus grypus) were exposed to a low doses of a cell culture-propagated phocine distemper virus isolate (pdv 2558/han 88). an intranasal route of inoculation was chosen. clinical signs, resembling those of 1988's seal disease and seroconversion were observed in both seronegative harbour seals. one of them succumbed to the infection. the virus was not transmitted to anot ... | 1990 | 2267888 |
| distemper vaccination in ferrets. | 1990 | 2301133 | |
| [effect of maternally derived antibody levels on antibody responses to canine parvovirus, canine distemper virus and infectious canine hepatitis virus after vaccinations in beagle puppies]. | antibody titers against canine parvovirus (cpv), canine distemper virus (cdv) and infectious canine hepatitis virus (ichv) in serum were measured in 6 beagle dams and their 38 puppies bred in our colony, in order to clarify the effects of maternally derived antibodies to antibody responses against the viruses after vaccinations in puppies. correlation coefficient on antibody titers between puppies and dams were cpv: r = 0. 7935, cdv: r = 0.8194 and ichv: r = 0.8105. mean maternal antibody positi ... | 1990 | 2303100 |
| molecular cloning of the rinderpest virus matrix gene: comparative sequence analysis with other paramyxoviruses. | the nucleotide sequence of the gene encoding the matrix or membrane (m) protein of the virulent (kabete-o) strain of rinderpest virus (rpv) has been determined. the m gene is 1457 nucleotides long with a single, large open reading frame. the derived polypeptide has 335 amino acids, corresponding to a calculated molecular weight of 38,289 and contains both small hydrophobic regions and many basic residues. the predicted amino acid sequence was compared to the m proteins of paramyxoviruses. sequen ... | 1990 | 2309449 |
| a case report: encephalitis in lions. pathological and virological findings. | an acute outbreak of encephalomyelitis in lions from a safari-park was investigated. three moribund lions were euthanatized and a post mortem examination was performed. a disseminated non-suppurative polioencephalomyelitis with demyelination in the spinal cord was the only pathological finding. from tonsil material of one lion feline herpesvirus type 1 was isolated. canine distemper virus, pseudorabies virus, feline infectious peritonitis virus and rabies were excluded as cause of the disease. t ... | 1990 | 2311536 |
| vaccines authorized for use in ferrets. | 1990 | 2321346 | |
| sequence characterization of the membrane protein gene of paramyxovirus simian virus 5. | the complete nucleotide sequence of the membrane (m) protein gene of the paramyxovirus simian virus 5 (sv5) was determined from cdna clones of viral mrnas. the m gene boundaries were determined by (i) primer extension sequencing on m mrna; (ii) nuclease s1 analysis; and (iii) primer extension sequencing on viral genomic rna. the m gene mrna consisted of 1371 templated nucleotides. it contains a single large open reading frame that can encode a protein of 377 amino acids with a predicted mr = 42, ... | 1990 | 2330672 |
| growth of canine distemper virus in cultured astrocytes: relationship to in vivo persistence and disease. | canine distemper virus (cdv) causes an encephalomyelitis in dogs which varies with the viral strain. the cdv cornell a75-17 strain produces a delayed, subacute to chronic, demyelinating cns disease. in contrast, the snyder hill (cdv-sh) strain-associated neurological disease is more acute in onset, is usually non-demyelinating and primarily produces lesions in the gray matter. in these studies we describe the effects of these two virulent and one avirulent cdv strain, rockborn (cdv-ro), on astro ... | 1990 | 2333034 |
| encephalitis induced by a canine distemper virus in squirrel monkeys. | intracerebral inoculation with canine distemper virus (cdv) caused acute neurological signs of viral encephalitis in squirrel monkeys. electroencephalogram revealed an abnormal sharp wave and seizure discharges resembling those of epilepsy. there was parenchymal inflammation, perivascular cuffs, neuronal degeneration, and glial reactions. virus antigen was detected immunohistologically in the neurons and ependymal cells. thus, cdv infection in squirrel monkeys provides an animal model for viral ... | 1990 | 2338709 |
| the role of the 5' nontranslated regions of the fusion protein mrnas of canine distemper virus and rinderpest virus. | the mrnas which code for the fusion proteins of the morbilliviruses (measles virus, canine distemper virus, and rinderpest virus) have unusually long 5' untranslated regions (utrs) which are gc-rich and are capable of folding into extensive secondary structures. in measles virus the first aug codons in the fusion (f) protein mrna are in close proximity at nucleotide positions 574 and 583 and protein translation is initiated at the second position. in the canine distemper virus (cdv) and rinderpe ... | 1990 | 2353458 |
| ultrastructural and biochemical findings in brain cell cultures infected with canine distemper virus. | to study the pathomechanism of demyelination in canine distemper (cd), dog brain cell cultures were infected with virulent a75/17-cd virus (cdv) and examined ultrastructurally. special attention was paid to the oligodendrocytes, which were specifically immunolabelled. in addition, cerebroside sulfotransferase (cst), an enzyme specific for oligodendrocyte activity was assayed during the course of the infection. infection and maturation as well as cdv-induced changes were found in astrocytes and b ... | 1990 | 2360417 |
| [creation of an experimental cell culture vaccine against distemper using cell line 4647]. | experiment in susceptible animals demonstrated the effectiveness and safety of several samples of a distemper vaccine prepared in cell line 4647. preparations of the vaccine virus variants generated in line 4647 after 10 passages at 34 degrees c, 37 degrees c and 40 degrees c and 20 passages of rapidly multiplying virus (34 degrees c) possessed the protective activity when used for immunization in a dose of 1000 pfu/animal. the results obtained in observations of the animals and examinations of ... | 1990 | 2363275 |
| [optimum conditions for the reproduction of distemper virus in cell line 4647]. | 1990 | 2363279 | |
| histopathological findings in harbour seals (phoca vitulina) found dead on the german north sea coast. | various organs--lung, trachea, liver, kidney, heart, adrenal gland, skin, spleen, thymus, lymph node, gut, thyroid, spinal cord and brain--were removed from 43 seals at dissections performed on the german north sea coast. the specimens were fixed in formalin and routinely processed for light microscopy. the major pathological findings were lung: acute congestion with interstitial and intra-alveolar oedema; intra-alveolar haemorrhage; suppurative bronchitis and bronchopneumonia; larvae and adult ... | 1990 | 2365846 |
| interaction of canine distemper virus nucleocapsid variants with 70k heat-shock proteins. | cytoplasmic nucleocapsid (nc) isolated from vero (v) cells infected in the logarithmic phase of growth with onderstepoort canine distemper virus consists of light-nc (l-nc) and dense-nc (d-nc), encapsidating full-length genomic rna, and defective-nc (df-nc), encapsidating variably truncated rnas. the 70k host cell protein constituent of l-nc and df-nc was shown to be a member of the 70k heat-shock protein (70k hsp) family. specifically, 72k hsp is associated with l-nc, and 72k and 73k hsp are as ... | 1990 | 2374009 |
| humoral immune responses in seals infected by phocine distemper virus. | recently the isolation and characterisation of a morbillivirus which caused high mortality in common seals (phoca vitulina) in 1988 have been reported. because of the clinical and pathological similarity of the disease in seals to that of distemper in dogs, the name phocine distemper virus (pdv) has been proposed. there are marked differences in the virus-induced proteins of pdv compared to other morbilliviruses and the humoral immune response of moribund and dead seals to pdv was restricted to ... | 1990 | 2382048 |
| vaccine-associated canine distemper infection in a litter of african hunting dogs (lycaon pictus). | four, 57 days old, african hunting dog puppies (lycaon pictus) from one litter died within three weeks following vaccination with modified-live canine distemper virus (cdv) and killed canine adenovirus type 1, canine parvovirus and leptospira icterohemorrhagiae and canicola. 18 days post vaccination, the animals developed neurologic disease characterized by episodes of grand mal seizures and circling. macroscopic, histological and immunohistochemical studies revealed acute systemic cdv infection ... | 1990 | 2385981 |
| antiserum raised in pigs against canine distemper virus and its utility in diagnostic procedures for morbillivirus infections (canine distemper, phocine distemper, rinderpest). | antiserum against canine distemper virus (cdv) was raised in pigs by intranasal inoculation with cdv strains cnd65 and rockborn. immunoglobulin fractions were conjugated with horseradish peroxidase. peroxidase-conjugated anti-cdv immunoglobulin preparations were used for the detection and titration of cdv, seal-derived (phocine) distemper virus (pdv) and rinderpest virus (rpv) in vero cell cultures. for the detection and titration of corresponding neutralizing antibodies a direct neutralizing pe ... | 1990 | 2396484 |
| is rinderpest virus the archevirus of the morbillivirus genus? | groups of 6-39 monoclonal antibodies identifying 3-18 distinct epitopes on the nucleoprotein (np), polymerase (p), hemagglutinin (h; equivalent in canine distemper and rinderpest viruses), and fusion (f) components of measles and canine distemper viruses were characterized in immunofluorescence tests with fixed vero cell cultures infected with measles, canine distemper and rinderpest viruses. the majority of np-specific monoclonal antibodies reacted with all three viruses, but one-third of the a ... | 1985 | 2409055 |
| correlation between epitopes on hemagglutinin of measles virus and biological activities: passive protection by monoclonal antibodies is related to their hemagglutination inhibiting activity. | measles virus monoclonal antibodies (moabs) were used to investigate the structure of the hemagglutinin (h) antigen, in order to study the regions of the molecule implicated in protection. using a competition binding assay, three overlapping domains were defined, and these have been correlated with the biological activities of their corresponding moab. all of the anti-h moabs, with a single exception, neutralized virus infectivity in vitro. we investigated their capacity in passive protection us ... | 1985 | 2414919 |
| in vitro and in vivo properties of the virus causing natural canine distemper encephalitis. | a group of dogs with naturally occurring canine distemper developed prodromal systemic symptoms followed by neurological disorders. the post-infection courses of these diseases lasted approximately 2 months. a varying degree of demyelination and inclusion body formation was found mostly in the cerebella of virologically confirmed cases with little or no inflammatory response. the distribution of canine distemper virus antigen coincided with the histopathological lesions. the animals had moderate ... | 1986 | 2419490 |
| secondary degeneration of oligodendrocytes in canine distemper virus infection in vitro. | to study the pathogenesis of demyelination in canine distemper virus (cdv) infection, primary canine brain cell cultures were infected with cdv to examine specific virus-induced glial cell changes. cultures were harvested at regular intervals after inoculation and were immunostained for the specific demonstration of astrocytes, oligodendrocytes, and cdv antigen. the infection spread slowly with moderate cytolysis and cell fusion. soon after inoculation, infection of astrocytes was found by means ... | 1986 | 2421102 |
| the antigenic relationship between measles, canine distemper and rinderpest viruses studied with monoclonal antibodies. | monoclonal antibodies (mabs) were used to delineate the antigenic relationship between the three morbillivirus types: measles virus (mv), canine distemper virus (cdv) and rinderpest virus (rpv). panels of six to 31 mabs against the haemagglutinin (h), fusion (f), nucleocapsid protein (np), phosphoprotein (p) and matrix (m) proteins of mv and the h, f, np and p proteins of cdv were employed. nine strains of mv, three strains of cdv and four strains of rpv were examined by radioimmunoprecipitation ... | 1986 | 2425047 |
| demyelinating canine distemper encephalomyelitis: measurement of myelin basic protein in cerebrospinal fluid. | beagle dogs were experimentally infected with the cornell a75-17 strain of canine distemper virus. at three time points post-infection (pi), immunoreactive myelin basic protein (mbp) was measured in cerebrospinal fluid (csf). levels were correlated with neuropathological findings, interferon in csf and virus isolation from the brain. csf from animals inoculated with cornell a75-17 strain often showed detectable immunoreactive mbp late in the disease course. as anticipated from earlier morphologi ... | 1987 | 2434527 |
| demyelinating, non-demyelinating and attenuated canine distemper virus strains induce oligodendroglial cytolysis in vitro. | a virulent canine distemper virus (cdv) strain that causes demyelination in vivo has been shown to induce oligodendroglial degeneration in vitro. in order to investigate if this effect on oligodendrocytes is specific for demyelinating strains only, primary brain cell cultures were infected with either virulent demyelinating strains (a75/17 and ch84-cdv), a virulent non-demyelinating strain (sh-cdv) or a non-virulent strain (op-cdv). all virulent viruses caused a persistent type infection with mo ... | 1987 | 2440997 |
| parainfluenza virus type 2 haemagglutinin-neuraminidase glycoprotein characterized with monoclonal antibodies. | thirteen monoclonal antibodies (mabs) were prepared against human parainfluenza virus type 2 (piv2). these mabs reacted with the haemagglutinin-neuraminidase glycoprotein with an mr of 84k. the mabs defined one antigenic site which could be divided into five epitopes. a correlation between haemagglutination inhibition (hi) and neutralization activity could be seen although one mab, which recognized a distinct epitope, showed neutralization and no hi activity to piv2. the reactivity of the mabs w ... | 1988 | 2451708 |
| bystander demyelination through antibody induced macrophage activation in canine distemper virus infection. | 1989 | 2467368 | |
| preparation and characterization of monoclonal antibodies to nucleocapsid protein n and h glycoprotein of rinderpest virus. | fifteen stable mouse spleen cell myeloma hybrids (hybridomas) producing monoclonal antibodies to rinderpest virus proteins were produced. the specificity of these monoclonal antibodies was established by radioimmunoprecipitation followed by polyacrylamide gel analysis and immunofluorescence. nine antibodies were specific for the surface glycoprotein h. all the nine clones showed inhibition of haemagglutination by measles virus. the antibodies from two clones (a7d2 and b2f6) neutralise infectious ... | 1989 | 2471370 |
| electrophoretic determination of albumin and gamma globulin concentrations in the cerebrospinal fluid of dogs with encephalomyelitis attributable to canine distemper virus infection: 13 cases (1980-1987). | thirteen dogs with encephalomyelitis attributable to canine distemper virus infection were classified into 3 groups on the basis of histopathologic evidence of virus-induced lesions in cns tissue. analysis of data indicated a similarity within groups when arranged by age, clinical neurologic signs, and alterations in the csf electrophoretic protein values. comparison with results in dogs with experimentally induced canine distemper encephalomyelitis suggested that when grouped by age, csf electr ... | 1989 | 2477352 |
| characterization of monoclonal antibodies against four structural proteins of rinderpest virus. | twenty-four monoclonal antibodies (mabs) against rinderpest virus (rpv) were established and characterized by several serological tests. of the 24 mabs. 10 recognized the nucleoprotein (np), six the phosphoprotein (p), four the haemagglutinin (h), and two the fusion (f) protein as determined by radioimmunoprecipitation assay. the specificities of the remaining two mabs could not be determined. from a competitive binding assay using mabs against each structural protein, at least five, four and tw ... | 1989 | 2477496 |
| quantitation of canine distemper virus and antibodies by enzyme-linked immunosorbent assays using protein a and monoclonal antibody capture. | monoclonal antibodies produced from 19 cloned hybridomas were selected for this study. specific canine distemper virus (cdv) antibodies in medium from cloned hybridomas were detected by direct enzyme-linked immunosorbent assays (elisa) and by indirect immunofluorescence. three different sandwich elisa systems were developed either to detect cdv in cell cultures and clinical specimens or to detect specific antibody in canine sera. protein a and monoclonal antibodies attached in sequence to a soli ... | 1989 | 2488329 |
| distemper-like disease and encephalitozoonosis in wild dogs (lycaon pictus). | clinical signs of a fatal disease resembling those of canine distemper were observed in two groups of captive wild dog (lycaon pictus) pups 13 days after vaccination with a commercially available combination vaccine for dogs which contained a live attenuated strain of canine distemper virus. histopathological examination of tissues revealed the presence of intranuclear inclusion bodies in neurons and lesions resembling canine distemper as well as colonies of an encephalitozoon sp. in the central ... | 1989 | 2492610 |
| serologic response of domestic ferrets (mustela putorius furo) to canine distemper and rabies virus vaccines. | nine unrelated 12-week-old naive domestic ferrets (mustela putorius furo) were used to evaluate the serologic responses to commercial canine distemper virus (cdv) and rabies virus (rv) vaccines. five of the ferrets (group 1) were inoculated 3 times at 2-week intervals with a multivalent modified-live virus vaccine of canine cell-line origin, containing cdv and an inactivated rv vaccine. four of the ferrets (group 2) were inoculated once with the multivalent modified-live virus vaccine containing ... | 1989 | 2537273 |
| effects of vaccines on the canine immune system. | the effects of several commercially available polyvalent canine vaccines on the immune system of the dog were examined. the results demonstrated that the polyvalent vaccines used in this study significantly suppressed the absolute lymphocyte count and that most of the polyvalent vaccines significantly suppressed lymphocyte response to mitogen, but had no effect on natural effector cell activity, neutrophil chemiluminescence, nor antibody response to canine distemper virus. the individual vaccine ... | 1989 | 2540897 |
| preparation and characterization of monoclonal antibodies directed against four structural components of canine distemper virus. | mouse hybridomas producing antibodies against structural proteins of canine distemper virus (cdv) were produced by fusion of sp2/0 myeloma cells with spleen cells from balb/c mice immunized with purified preparations of vero cell-grown cdv. ascites fluids collected after intraperitoneal inoculation with 149 cdv antibody-producing hybridoma cell lines were characterized by different serological tests. by immune precipitation tests with [35s]methionine-labelled extracellular virions and intracellu ... | 1985 | 2579191 |
| isolation and characterization of canine distemper virus-specific rna. | ten species of virus-specific rna were detected in vero cells infected with the fxno strain of canine distemper virus (cdv). the largest rna was the genome-sized rna and the nine smaller species were polyadenylated rnas. similar results were obtained for nine other strains of cdv. the molecular weights of these ten rnas were determined to be 4.61 x 10(6), 2.46 x 10(6), 1.52 x 10(6), 1.32 x 10(6), 1.19 x 10(6), 1.07 x 10(6), 0.77 x 10(6), 0.65 x 10(6), 0.58 x 10(6), and 0.48 x 10(6). by in vitro ... | 1985 | 2581115 |
| a serious outbreak of canine distemper among sled-dogs in northern greenland. | a canine distemper outbreak in a highly susceptible sled dog population of northern greenland was recognized in the beginning of january 1988. a high morbidity and mortality in all age groups was a characteristic of the epizootic. the actual canine distemper virus infection was identified by isolation of the virus and demonstration of viral antigens by immunofluorescence and also by demonstration of conventional inclusion bodies. virus specific igm antibodies were demonstrated in affected dogs. ... | 1989 | 2590318 |
| morbillivirus infection of seals (phoca vitulina) during the 1988 epidemic in the bay of heligoland. i. mode, frequency and significance of cultural virus isolation and neutralizing antibody detection. | from 16 (14%) out of 112 dead or euthanized seals originating from wildlife and seal orphanages phocine morbillivirus was isolated. the majority of viral isolates in cell cultures was obtained from lung homogenates of 15 out of 71 free-ranging seals (21%). the virus was isolated by longterm cultivation in roller cultures of seal kidney cells. the phocine morbillivirus was detected by typical cytopathogenic alteration and by peroxidase-linked antibody (pla) assay, respectively. a neutralization t ... | 1989 | 2596201 |
| canine distemper virus infection in lesser pandas (ailurus fulgens). | 1989 | 2601238 |