Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| molecular genetic analysis and evolution of segment 7 in rice black-streaked dwarf virus in china. | rice black-streaked dwarf virus (rbsdv) causes maize rough dwarf disease or rice black-streaked dwarf disease and can lead to severe yield losses in maize and rice. to analyse rbsdv evolution, codon usage bias and genetic structure were investigated in 111 maize and rice rbsdv isolates from eight geographic locations in 2013 and 2014. the linear dsrna s7 is a+u rich, with overall codon usage biased toward codons ending with a (a3s, s7-1: 32.64%, s7-2: 29.95%) or u (u3s, s7-1: 44.18%, s7-2: 46.06 ... | 2015 | 26121638 |
| whole-genome expression analysis of rice black-streaked dwarf virus in different plant hosts and small brown planthopper. | rice black-streaked dwarf virus (rbsdv) can infect a number of gramineous plants and cause severe crop yield losses in southeast asian countries. the virus is transmitted by small brown planthopper (sbph) in a persistent circulative manner. the interactions between rbsdv and its different hosts remain unknown. besides, how the virus adjusts itself to infect different hosts is unclear. in the present study, the relative rna levels of the thirteen rbsdv genes in rice, maize, wheat, and sbph were m ... | 2015 | 26149652 |
| genetic analysis and molecular mapping of qtls for resistance to rice black-streaked dwarf disease in rice. | rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (rbsdv), is transmitted by small brown planthoppers (laodelphax striatellus fallén, sbph) and causes severe yield loss in epidemic years in china and other east asian countries. breeding for resistance to rbsdv is a promising strategy to control the disease. we identified tetep that showed resistance to rbsdv using a field test and artificial inoculation test. an evaluation of the resistance mechanism revealed that tete ... | 2015 | 26198760 |
| identification and expression profiling analysis of calmodulin-binding transcription activator genes in maize (zea mays l.) under abiotic and biotic stresses. | the calmodulin-binding transcription activators (camta) play critical roles in plant growth and responses to environmental stimuli. however, how camtas function in responses to abiotic and biotic stresses in maize (zea mays l.) is largely unknown. in this study, we first identified all the camta homologous genes in the whole genome of maize. the results showed that nine zmcamta genes showed highly diversified gene structures and tissue-specific expression patterns. many zmcamta genes displayed h ... | 2015 | 26284092 |
| interactive effects of southern rice black-streaked dwarf virus infection of host plant and vector on performance of the vector, sogatella furcifera (homoptera: delphacidae). | performance of insect vectors can be influenced by the viruses they transmit, either directly by infection of the vectors or indirectly via infection of the host plants. southern rice black-streaked dwarf virus (srbsdv) is a propagative virus transmitted by the white-backed planthopper, sogatella furcifera (hovath). to elucidate the influence of srbsdv on the performance of white-backed planthopper, life parameters of viruliferous and nonviruliferous white-backed planthopper fed rice seedlings i ... | 2014 | 26309259 |
| rice responses and resistance to planthopper-borne viruses at transcriptomic and proteomic levels. | rice (oryza sativa) is one of the most important cereal crops in the world, especially in asian areas. rice virus diseases are considered as the most serious threat to rice yields. most rice viruses are transmitted by hemipteran insects such as planthoppers and leafhoppers. in asia five rice viruses are transmitted mainly by three planthopper species in a persistent manner: rice stripe virus, rice black-streaked dwarf virus, rice ragged stunt virus, rice grassy stunt virus, and southern rice bla ... | 2016 | 26363817 |
| low temperature storage of southern rice black-streaked dwarf virus-infected rice plants cannot sustain virus transmission by the vector. | southern rice black-streaked dwarf virus (srbsdv) is a novel virus transmitted by white-backed planthopper sogatella furcifera (hováth) (hemiptera: delphacidae). due to low virus transmission efficiency by the planthopper, researchers are frequently confronted with shortage of viruliferous vectors or infected rice plants, especially in winter and the following spring. to find new ways to maintain virus-infected materials, viral rice plants were stored at -80°c for 45 or 140 d and evaluated as vi ... | 2016 | 26405060 |
| nac transcription factor family genes are differentially expressed in rice during infections with rice dwarf virus, rice black-streaked dwarf virus, rice grassy stunt virus, rice ragged stunt virus, and rice transitory yellowing virus. | expression levels of the nac gene family were studied in rice infected with rice dwarf virus (rdv), rice black-streaked dwarf virus (rbsdv), rice grassy stunt virus (rgsv), rice ragged stunt virus (rrsv), and rice transitory yellowing virus (rtyv). microarray analysis showed that 75 (68%) osnac genes were differentially regulated during infection with rdv, rbsdv, rgsv, and rrsv compared with the control. the number of osnac genes up-regulated was highest during rgsv infection, while the lowest n ... | 2015 | 26442000 |
| argonaute subfamily genes in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | argonaute (ago) proteins are essential catalytic components of the rna-induced silencing complex and play central roles in rna interference. using a combination of bioinformatics and rapid amplification of cdna ends (race) methods, putative ago subfamily members, ls-ago1 and ls-ago2, were cloned and characterized from the small brown planthopper, laodelphax striatellus. the open reading frame (orf) of ls-ago1 is 2,820 bp long, encoding a putative protein of 939 amino acid residues, and ls-ago2 c ... | 2016 | 26446351 |
| relation between the viral load accumulation of southern rice black-streaked dwarf virus and the different developmental stages of sogatella furcifera (hemiptera: delphacidae). | the white-backed planthopper, sogatella furcifera (horvath), is currently the only confirmed vector of southern rice black-streaked dwarf virus (srbsdv), which causes severe rice production losses in china. in this study, an absolute quantification qpcr method was used to detect viral gene mrna expression levels at different developmental stages of white-backed planthoppers fed srbsdv-infected rice plants. a comparison of viral copy numbers of the srbsdv s10 gene at the same developmental stage ... | 2015 | 26470211 |
| identification and profiling of conserved and novel micrornas in laodelphax striatellus in response to rice black-streaked dwarf virus (rbsdv) infection. | micrornas (mirnas) are small non-coding endogenous rna molecules that play important roles in various biological processes. this study examined microrna profiles of laodelphax striatellus using the small rna libraries derived from virus free (vf) and rice black-streaked dwarf virus (rbsdv) infected (rb) insects. a total of 59 mature mirnas (46 mirna families) were identified as conserved insect mirnas in both vf and rb libraries. among these conserved mirnas, 24 were derived from the two arms of ... | 2014 | 26484150 |
| assessment of reference gene stability in rice stripe virus and rice black streaked dwarf virus infection rice by quantitative real-time pcr. | stably expressed reference gene(s) normalization is important for the understanding of gene expression patterns by quantitative real-time pcr (rt-qpcr), particularly for rice stripe virus (rsv) and rice black streaked dwarf virus (rbsdv) that caused seriously damage on rice plants in china and southeast asia. | 2015 | 26497487 |
| small interfering rna pathway modulates initial viral infection in midgut epithelium of insect after ingestion of virus. | numerous viruses are transmitted in a persistent manner by insect vectors. persistent viruses establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles. although propagation of viruses in insect vectors can be controlled by the small interfering rna (sirna) antiviral pathway, whether the sirna pathway can control viral dissemination from the midgut epithelium is unknown. infection by a rice virus (southern rice black streaked dwarf vir ... | 2015 | 26537672 |
| reference gene selection and evaluation for gene expression studies using qrt-pcr in the white-backed planthopper, sogatella furcifera (hemiptera: delphacidae). | the white-backed planthopper, sogatella furcifera (hemiptera, delphacidae), is one of the most devastating rice pests. for a better control strategy, various genetic studies have been conducted using reverse-transcription quantitative real-time polymerase chain reaction (qrt-pcr). the appropriate application of qrt-pcr requires reliable endogenous controls; however, studies on this aspect of the white-backed planthopper are lacking. in the present study, nine commonly used reference genes, elong ... | 2016 | 26612891 |
| maize-pathogen interactions: an ongoing combat from a proteomics perspective. | maize (zea mays l.) is a host to numerous pathogenic species that impose serious diseases to its ear and foliage, negatively affecting the yield and the quality of the maize crop. a considerable amount of research has been carried out to elucidate mechanisms of maize-pathogen interactions with a major goal to identify defense-associated proteins. in this review, we summarize interactions of maize with its agriculturally important pathogens that were assessed at the proteome level. employing diff ... | 2015 | 26633370 |
| complete genomic sequence of maize rough dwarf virus, a fijivirus transmitted by the small brown planthopper. | the nucleotide sequences of the 10 genomic segments of an italian isolate of maize rough dwarf virus (mrdv) were determined. this first complete genomic sequence of mrdv will help understand the phylogenetic relationships among group 2 fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize. | 2016 | 26847903 |
| [development of transgenic maize with anti-rough dwarf virus artificial mirna vector and their disease resistance]. | maize is one of the most important food crops. rice black-streaked dwarf virus is a maize rough dwarf disease pathogen. the occurrence and transmission of maize rough dwarf disease brings great damage to maize production. the technology of using artificial mirna to build antiviral plant has been proven effective in a variety of plants. however, such trials in maize have not been reported. we designed primers based on the sequence of maize zea-mir159a precursor and sequence of function protein ge ... | 2015 | 26955715 |
| identification and characterization of micrornas in the white-backed planthopper, sogatella furcifera. | micrornas (mirnas) are a novel class of small, non-coding endogenous rnas that play critical regulatory roles in many metabolic activities in eukaryotes. reports of the identification of mirnas in sogatella furcifera (white-backed planthopper), the insect that acts as the only confirmed vector of the southern rice black-streaked dwarf virus (srbsdv), are limited. in this study, a total of 382 mirnas were identified in s. furcifera, including 106 conserved and 276 novel mirnas, using high-through ... | 2016 | 27060479 |
| hairpin rna targeting multiple viral genes confers strong resistance to rice black-streaked dwarf virus. | rice black-streaked dwarf virus (rbsdv) belongs to the genus fijivirus in the family of reoviridae and causes severe yield loss in rice-producing areas in asia. rna silencing, as a natural defence mechanism against plant viruses, has been successfully exploited for engineering virus resistance in plants, including rice. in this study, we generated transgenic rice lines harbouring a hairpin rna (hprna) construct targeting four rbsdv genes, s1, s2, s6 and s10, encoding the rna-dependent rna polyme ... | 2016 | 27187354 |
| a phloem-limited fijivirus induces the formation of neoplastic phloem tissues that house virus multiplication in the host plant. | a number of phloem-limited viruses induce the development of tumours (enations) in the veins of host plants, but the relevance of tumour induction to the life cycle of those viruses is unclear. in this study, we performed molecular and structural analyses of tumours induced by rice black-streaked dwarf virus (rbsdv, genus fijivirus) infection in maize plants. the transcript level of the maize cdc2 gene, which regulates the cell cycle, was highly elevated in tumour tissues. two-dimensional electr ... | 2016 | 27432466 |
| epg recordings reveal differential feeding behaviors in sogatella furcifera in response to plant virus infection and transmission success. | plant viruses are primarily transmitted by insect vectors and virus infection may influence on the vectors' feeding behaviors. using an electrical penetration graph, we detected that infection with the southern rice black-streaked dwarf virus (srbsdv) in the white-backed planthopper (wbph) and in rice plants both altered the vector's feeding behavior. when viruliferous wbph (carrying srbsdv) were fed on uninfected plants, they spent more time in salivation and phloem sap ingestion than non-virul ... | 2016 | 27492995 |
| dual transcriptome analysis reveals insights into the response to rice black-streaked dwarf virus in maize. | maize rough dwarf disease (mrdd) is a viral infection that results in heavy yield losses in maize worldwide, particularly in the summer maize-growing regions of china. mrdd is caused by the rice black-streaked dwarf virus (rbsdv). in the present study, analyses of micrornas (mirnas), the degradome, and transcriptome sequences were used to elucidate the rbsdv-responsive pathway(s) in maize. genomic analysis indicated that the expression of three non-conserved and 28 conserved mirnas, representing ... | 2016 | 27493226 |
| tolerance and responsive gene expression of sogatella furcifera under extreme temperature stresses are altered by its vectored plant virus. | southern rice black-streaked dwarf virus (srbsdv), a newly emerged fijivirus causing great loss to rice production in eastern and southeastern asian countries in recent years, is efficiently transmitted by a rice pest, white-backed planthopper (wbph, sogatella furcifera) in a persistent, circulative propagative manner and can be considered as an insect virus. in this study, srbsdv infection in wbph was found to increase the vector's death rate under extreme cold stress but improve its survival r ... | 2016 | 27531640 |
| molecular variation and expansion of a rice black-streaked dwarf virus population based on analysis of segment 1 in jining, china. | to analyze the variation in rice black-streaked dwarf virus (rbsdv) in an area with high incidence of maize rough dwarf disease (mrdd), the rbsdv s1 segment in a collection of 100 maize isolates (sample population a100) from jining, shandong province, was sequenced. an additional 21 maize and rice isolates (subpopulation b21) that were sampled from nine other geographic locations in china in 2012 and 2013 were used as a control. a total of 914 nucleotide mutations, including 239 singleton variab ... | 2016 | 27638775 |
| analysis of sogatella furcifera proteome that interact with p10 protein of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv) is transmitted efficiently only by white-backed planthopper (wbph, sogatella furcifera) in a persistent propagative manner. here we used a yeast two-hybrid system to investigate the interactions between the srbsdv- p10 and the cdna library of wbph. of 130 proteins identified as putative interactors, 28 were further tested in a retransformation analysis and β-galactosidase assay to confirm the interaction. the full-length gene sequences of 5 candi ... | 2016 | 27653366 |
| asymmetric spread of srbsdv between rice and corn plants by the vector sogatella furcifera (hemiptera: delphacidae). | plant viruses are mostly transmitted by sucking insects via their piercing behaviors, which may differ due to host plant species and their developmental stages. we characterized the transmission of a fijivirus, southern rice black-streaked dwarf virus (srbsdv), by the planthopper vector sogatella furcifera horváth (hemiptera: delphacidae), between rice and corn plants of varying developmental stages. srbsdv was transmitted from infected rice to uninfected corn plants as efficiently as its transm ... | 2016 | 27760223 |
| the speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors. | rice black-streaked dwarf virus (rbsdv) and southern rice black-streaked dwarf virus (srbsdv) are two closely related fijiviruses transmitted by the small brown planthopper (sbph) and white-backed planthopper (wbph), respectively. srbsdv has a latent period 4 days shorter than that of rbsdv, implying a more efficient spread in insect vector. currently, the mechanisms underlying this higher efficiency are poorly understood. however, our recent studies have implicated a role of virus induced tubul ... | 2016 | 27760544 |
| understanding the immune system architecture and transcriptome responses to southern rice black-streaked dwarf virus in sogatella furcifera. | sogatella furcifera, the white-backed planthopper (wbph), has become one of the most destructive pests in rice production owing to its plant sap-sucking behavior and efficient transmission of southern rice black-streaked dwarf virus (srbsdv) in a circulative, propagative and persistent manner. the dynamic and complex srbsdv-wbph-rice plant interaction is still poorly understood. in this study, based on a homology-based genome-wide analysis, 348 immune-related genes belonging to 28 families were ... | 2016 | 27805032 |
| characterization of small interfering rnas derived from rice black streaked dwarf virus in infected maize plants by deep sequencing. | rice black streaked dwarf virus (rbsdv) is the casual agent of maize rough dwarf disease, which frequently causes severe yield loss in china. however, the interaction between rbsdv and maize plants is largely unknown. rna silencing is a conserved mechanism against viruses in plants. to understand the antiviral rna interfering response in rbsdv-infected plants, the profile of virus-derived small interfering rnas (vsirnas) from rbsdv in infected maize plants was obtained by deep sequencing in this ... | 2017 | 27888127 |
| rnai-mediated resistance to rice black-streaked dwarf virus in transgenic rice. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, causes significant economic losses in rice production in china and many other asian countries. development of resistant varieties by using conventional breeding methods is limited, as germplasm with high level of resistance to rbsdv have not yet been found. one of the most promising methods to confer resistance against rbsdv is the use of rna interference (rnai) technology. rbsdv non-structural pro ... | 2017 | 27900537 |
| virus altered rice attractiveness to planthoppers is mediated by volatiles and related to virus titre and expression of defence and volatile-biosynthesis genes. | viruses may induce changes in plant hosts and vectors to enhance their transmission. the white-backed planthopper (wbph) and brown planthopper (bph) are vectors of southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv), respectively, which cause serious rice diseases. we herein describe the effects of srbsdv and rrsv infections on host-selection behaviour of vector and non-vector planthoppers at different disease stages. the y-tube olfactometer choice and free-choic ... | 2016 | 27924841 |
| jasmonic acid-mediated defense suppresses brassinosteroid-mediated susceptibility to rice black streaked dwarf virus infection in rice. | plant hormones play a vital role in plant immune responses. however, in contrast to the relative wealth of information on hormone-mediated immunity in dicot plants, little information is available on monocot-virus defense systems. we used a high-throughput-sequencing approach to compare the global gene expression of rice black-streaked dwarf virus (rbsdv)-infected rice plants with that of healthy plants. exogenous hormone applications and transgenic rice were used to test rbsdv infectivity and p ... | 2017 | 27976810 |
| interaction between southern rice black-streaked dwarf virus minor core protein p8 and a rice zinc finger transcription factor. | the fijivirus southern rice black-streaked dwarf virus (srbsdv) causes one of the most serious viral diseases of rice in china and vietnam. to better understand the molecular basis of srbsdv infection, a yeast two-hybrid screen of a rice cdna library was carried out using p8, a minor core protein of srbsdv, as the bait. a rice cys2his2-type zinc finger protein (oszfp) was found to interact with srbsdv p8. a strong interaction between srbsdv p8 and oszfp was then confirmed by pull-down assays, an ... | 2017 | 28124144 |
| characteristics of sirnas derived from southern rice black-streaked dwarf virus in infected rice and their potential role in host gene regulation. | virus-derived sirnas (vsirnas)-mediated rna silencing plays important roles in interaction between plant viruses and their hosts. southern rice black-streaked dwarf virus (srbsdv) is a newly emerged devastating rice reovirus with ten dsrna genomic segments. the characteristics of srbsdv-derived sirnas and their biological implications in srbsdv-rice interaction remain unexplored. | 2017 | 28183327 |
| evaluation of rice resistance to southern rice black-streaked dwarf virus and rice ragged stunt virus through combined field tests, quantitative real-time pcr, and proteome analysis. | diseases caused by southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv) considerably decrease grain yield. therefore, determining rice cultivars with high resistance to srbsdv and rrsv is necessary. in this study, rice cultivars with high resistance to srbsdv and rrsv were evaluated through field trials in shidian and mangshi county, yunnan province, china. sybr green i-based quantitative real-time polymerase chain reaction (qrt-pcr) analysis was used to quantitat ... | 2017 | 28241456 |
| the influence of sogatella furcifera (hemiptera: delphacidae) migratory events on the southern rice black-streaked dwarf virus epidemics. | southern rice black-streaked dwarf virus (srbsdv) caused serious rice losses. after the first outbreak in 2009 in northern vietnam and southern china, the virus ravaged crops again on enormous scales in 2010, but infections have decreased sharply since 2011. we presumed that the sudden epidemics and fadeout of srbsdv would be closely related to the migratory events of the insect vector, sogatella furcifera. this study sought the source area of srbsdv using the trajectory analysis method, and rev ... | 2017 | 28334380 |
| structure and components of the globular and filamentous viroplasms induced by rice black-streaked dwarf virus. | viroplasms of members of the family reoviridae are considered to be viral factories for genome replication and virion assembly. globular and filamentous phenotypes have different components and probably have different functions. we used transmission electron microscopy and electron tomography to examine the structure and components of the two viroplasm phenotypes induced by rice black-streaked dwarf virus (rbsdv). immuno-gold labeling was used to localize each of the 13 rbsdv encoded proteins as ... | 2017 | 28359957 |
| development of a simplified rt-pcr without rna isolation for rapid detection of rna viruses in a single small brown planthopper (laodelphax striatellus fallén). | the small brown planthopper (sbph) is an important pest of cereal crops and acts as a transmission vector for multiple rna viruses. rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. reverse transcription polymerase chain reaction (rt-pcr) is a rapid, sensitive and reliable method for virus detection. the traditional rt-pcr contains a rna isolation step and is widely used for virus detection in insect. however, using the traditional rt-pcr for ... | 2017 | 28468626 |
| bacterial microbiota in small brown planthopper populations with different rice viruses. | the small brown planthopper (sbph) is an important virus vector, transmitting rice stripe virus (rsv), and rice black-streaked dwarf virus (rbsdv). insect symbionts play an essential role in the insect fitness, however, it is still unclear about their contributions to viral transmission by sbph. here, we investigated endosymbiont communities in non-viruliferous, rsv-infected, and rbsdv-infected sbph populations using illumina 16s rrna gene miseq sequencing. in total, 281,803 effective sequences ... | 2017 | 28471518 |
| rice black streaked dwarf virus p7-2 forms a scf complex through binding to oryza sativa skp1-like proteins, and interacts with gid2 involved in the gibberellin pathway. | as a core subunit of the scf complex that promotes protein degradation through the 26s proteasome, s-phase kinase-associated protein 1 (skp1) plays important roles in multiple cellular processes in eukaryotes, including gibberellin (ga), jasmonate, ethylene, auxin and light responses. p7-2 encoded by rice black streaked dwarf virus (rbsdv), a devastating viral pathogen that causes severe symptoms in infected plants, interacts with skp1 from different plants. however, whether rbsdv p7-2 forms a s ... | 2017 | 28494021 |
| interaction research on an antiviral molecule that targets the coat protein of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv) coat protein (p10) is the key protein required for viral transmission and host plant infection and is thus a promising target for anti-srbsdv agent screening. in this study, p10 was obtained from escherichia coli through cloning, expression, and purification. the antiviral agent ningnanmycin was selected as control, and a series of antiviral compounds based on the structural scaffold of ferulic acid were analyzed. size-exclusion chromatography an ... | 2017 | 28522394 |
| label-free quantitative proteomic analysis of chitosan oligosaccharide-treated rice infected with southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv) has spread from thesouth of china to the north of vietnam in the past few years and severelyinfluenced rice production. its long incubation period and early symptoms are not evident; thus, controlling it is difficult. chitosan oligosaccharide (cos) is a green plant immunomodulator. early studies showed that preventing and controlling srbsdv have a certain effect and reduce disease infection rate, but its underlying controlling and preventing mech ... | 2017 | 28524115 |
| transcriptional changes of rice in response to rice black-streaked dwarf virus. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, causes significant economic losses in rice production in china and many other asian countries. although a great deal of effort has been made to elucidate the interactions among the virus, insect vectors, host and environmental conditions, few rbsdv proteins involved in pathogenesis have been identified, and the biological basis of disease development in rice remains largely unknown. transcriptomic ... | 2017 | 28700950 |
| co-infection of two reoviruses increases both viruses accumulation in rice by up-regulating of viroplasm components and movement proteins bilaterally and rna silencing suppressor unilaterally. | synergism between southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv) not only aggravates disease symptoms but also enhances their vector acquisition efficiencies by increasing both viruses' titers in co-infected rice plants, which may exacerbate the epidemic of both viruses and cause significant damage to rice production. the molecular mechanism of viral synergism of these two viruses remains unexplored. | 2017 | 28789694 |
| a novel method for transmitting southern rice black-streaked dwarf virus to rice without insect vector. | southern rice black-streaked dwarf virus (srbsdv) has spread from the south of china to the north of vietnam in the past few years, and has severely influenced rice production. however, previous study of traditional srbsdv transmission method by the natural virus vector, the white-backed planthopper (wbph, sogatella furcifera), in the laboratory, researchers are frequently confronted with lack of enough viral samples due to the limited life span of infected vectors and rice plants and low virus ... | 2017 | 28810884 |