Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| pathology of experimental cv777 coronavirus enteritis in piglets. i. histological and histochemical study. | sixteen cesarean-derived colostrum-deprived piglets were infected oronasally with cv777 coronavirus on the second or third day of life. two uninfected piglets were controls. they were killed at 96 and 120 hours after birth. after an incubation period of 22 to 36 hours, all principals showed severe diarrhea. the principals were killed between 12 and 120 hours after infection. exfoliation of enterocytes were seen first in the piglet killed 24 hours after infection (two hours after the diarrhea beg ... | 1982 | 6280359 |
| isolation and identification of swine rotavirus in taiwan. | large numbers of viral particles resembling rotavirus were detected with negative stained electron microscopy in bacteria free fecal filtrate obtained from 10-day old diarrheal suckling piglets of a conventional pig farm in taiwan. the clinical signs of vomiting and diarrhea were reproduced in colostrum deprived piglets artificially infected with the fecal filtrates. rotavirus particles persisted in the fecal samples after two in vivo serial passages, and was not seen in the uninfected control a ... | 1982 | 6291876 |
| rotaviral antibodies in cow's milk. | in the past, it has been reported that neonatal diets made from unheated cow's milk were superior to those made from heated cow's milk. it was observed that piglets were equally protected from rotaviral diarrhea when they were fed diets made from either unheated milk that came from a cow immunized against porcine rotavirus or from a cow that was not immunized. because of this observation, we examined four pools of "normal" cows' colostrum and 58 samples of "normal" cow's milk for the presence of ... | 1982 | 6293690 |
| electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus. | 1982 | 6296207 | |
| isolation and characterization of an equine rotavirus. | a rotavirus, designated as the h-1 strain, was isolated from a diarrheic foal in primary african green monkey kidney cells and ma104 cells. this cell culture-adapted strain hemagglutinated erythrocytes of human group o, rhesus monkeys, guinea pigs, and sheep. it was found to be similar, if not identical, to porcine rotaviruses (strains osu, ee, and a-580) by plaque reduction neutralization and hemagglutination inhibition tests, and, in addition, it was found to belong to subgroup 1. this equine ... | 1983 | 6313746 |
| isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. | seven rotavirus strains were isolated in cell cultures from the intestinal contents of piglets with diarrhea. ma104 cells with pancreatin in the cell culture medium was the host system of choice for virus isolation and replication. a cell culture immunofluorescence test in which ma104 cells were used in microtiter plates was very effective for detecting and assaying rotaviruses. a plaque reduction neutralization test, cross-protection studies in gnotobiotic pigs, and electrophoresis of rotaviral ... | 1984 | 6321541 |
| rotavirus associated diarrhoea in nursing piglets and detection of antibody against rotavirus in colostrum, milk and serum. | a blocking method of elisa for the detection and quantification of antibody against porcine rotavirus in serum, colostrum and milk has been compared with a plaque reduction test. the results obtained with the two techniques correlated (fig. 1). antibody against rotavirus was demonstrated in 384 serum samples representing 25 swine farms, indicating a widely spread and dense distribution of the infection with porcine rotavirus among danish swine. the antibody contents in milk samples from 7 gilts ... | 1983 | 6326047 |
| rotavirus excretion in suckling pigs followed under field circumstances. | the enzootic behaviour of porcine rotavirus in swine breeding farms was investigated by determination of the rotavirus excretion pattern in faeces using an enzyme linked immunosorbent assay (elisa). three herds were followed for 23, 14 and 9 consecutive weeks respectively and faeces were collected once a week from suckling piglets, weaned pigs and sows. the piglets of nearly all litters were found to excrete rotavirus in faeces before the age of 5-6 weeks. weaned pigs rarely excreted rotavirus a ... | 1983 | 6329062 |
| infection in piglets with a porcine rotavirus-like virus. experimental inoculation and ultrastructural examination. | a rotavirus-like virus has been isolated from cases of neonatal diarrhoea in piglets. no antigenic relationship with known rotaviruses or with an american strain of a rotavirus-like virus has been demonstrated. morphologically the virus is similar to known rotaviruses, but it differs in the ability to form syncytia of the enterocytes in the small intestine. | 1984 | 6329140 |
| pathogenesis of rotavirus-induced diarrhea. preliminary studies in miniature swine piglet. | the pathogenesis of diarrhea caused by rotavirus infection was studied in miniature swine piglets. the animals were inoculated orally with 2 x 10(7) plaque-forming units of porcine rotavirus (osu strain). during the height of diarrhea, intestinal function was investigated by in vivo perfusion of a 30-cm segment of proximal jejunum and a 30-cm segment of distal ileum. absorption of na+ and water decreased and 3-o-methylglucose transport was markedly reduced, p less than 0.01 compared to control a ... | 1984 | 6489082 |
| prevalence rate of porcine rotavirus in danish swine herds. | the prevalence rate of rotavirus in swine herds in denmark was examined. 28 intensive breeding herds were tested and rotavirus was detected in 18 herds, i.e. 64.2%. of the 638 fecal samples examined, 221 were positive (34.6%). the age distribution showed that rotavirus occurred with highest frequency (approx. 60%) in four week-old piglets. rotavirus infection in neonatal piglets predominantly occurred in litters born by gilts. production systems had influence on the occurrence of rotavirus. in t ... | 1983 | 6677181 |
| intestinal changes in gnotobiotic piglets experimentally inoculated with porcine rotavirus. | intestinal lesions were studied in 8 gnotobiotic piglets orally given porcine rotavirus by light microscopy, scanning electron microscopy and transmission electron microscopy. five of these piglets were killed 6, 12, 18, 24 and 48 hours after inoculation, respectively. of the other 3 piglets, one died 60 hours and two became moribund 96 and 114 hours after inoculation, respectively. all the infected piglets showed diarrhea between 18 and 24 hours after inoculation. examination of the small intes ... | 1982 | 7133149 |
| characterization of neutralization epitopes on the vp7 surface protein of serotype g11 porcine rotaviruses. | rotavirus strain a253, isolated from the faeces of a diarrhoeic piglet in venezuela, was classified as serotype g11 by cross-neutralization studies and by comparison of the deduced amino acid sequence of the vp7 surface protein. the epitopes involved in neutralization of the two g11 porcine rotavirus strains a253 and ym were analysed using neutralization-resistant mutants selected with seven neutralizing monoclonal antibodies (mabs), monotype-specific (m-) mabs and serotype-specific (s-) mabs, p ... | 1994 | 7519247 |
| mapping the subgroup epitopes of rotavirus protein vp6. | vp6, the most abundant protein of rotaviruses, contains epitopes that allow the classification of these viruses into four subgroups (sg), depending on the presence or absence of two epitopes called i and ii. the subgroup-specific epitopes are conformational and appear to be present on trimeric but not monomeric vp6. we have identified on vp6 some of the amino acids that determine the reactivity of the subgroup-specific mabs 255/60 and 631/9. a single amino acid mutation at positions 172 (met to ... | 1994 | 7522369 |
| characterization of the neutralizing epitopes of vp7 of the gottfried strain of porcine rotavirus. | the neutralization epitopes of the outer capsid protein vp7 of a porcine group a rotavirus were studied by using neutralizing monoclonal antibodies (n-mabs). six n-mabs which were specific for the vp7 protein of the gottfried strain of porcine rotavirus (serotype g4) were used for analyzing the antigenic sites of vp7. three different approaches were used for this analysis: testing the serological reactivity of each n-mab against different g serotypes of human and animal rotaviruses, analyzing n- ... | 1993 | 7691871 |
| comparative amino acid sequence analysis of the major outer capsid protein (vp7) of porcine rotaviruses with g3 and g5 serotype specificities isolated in venezuela and argentina. | seven porcine group a rotavirus strains isolated in venezuela were shown to be antigenically related to serotype g3 (five strains) or to serotype g5 (two strains), whereas two strains isolated in argentina were classified as serotype g5. the serological classification of eight of these strains was confirmed by sequence analysis of the gene encoding the vp7 glycoprotein. a high degree of homology was observed among strains belonging to the same g serotype, although some variations in the serotype ... | 1995 | 7733818 |
| identification of group a rotavirus genes associated with virulence of a porcine rotavirus and host range restriction of a human rotavirus in the gnotobiotic piglet model. | rotaviruses are the leading cause of severe diarrhea in infants and young children worldwide. thus, the development of an effective rotavirus vaccine is a major public health goal. this study was performed to identify the gene or genes responsible for rotavirus virulence or host range restriction and attenuation in a natural host. such knowledge could have an important bearing on the selection of candidate live vaccine strains. we addressed this issue by analyzing the response of gnotobiotic pig ... | 1995 | 7747480 |
| amino acid sequence of the porcine rotavirus ym vp1 protein. | 1994 | 7839009 | |
| vp6 from porcine rotavirus strain cn86: amino acid sequence divergence with conservation of subgroup ii specificity. | a porcine rotavirus strain, cn86, originally isolated from rotavirus-infected piglets in argentina, has been shown to possess unique characteristics. it was the first animal strain described to be antigenically related to human serotype g1 and the standard counterpart of another porcine strain showing rearrangement of genome segment 11. owing to these features, molecular characterization of this virus seemed relevant. the gene encoding the major inner capsid protein, vp6, was cloned and its nucl ... | 1995 | 7844537 |
| temporal correlation between a single amino acid change in the vp4 of a porcine rotavirus and a marked change in pathogenicity. | we previously described a marked increase in the pathogenicity of a cell culture grown porcine rotavirus, prv 4f, during serial passage in gnotobiotic piglets (bridger et al., 1992). here we report close temporal correlation between this change in pathogenicity and an amino acid change within a highly conserved hydrophobic domain of vp4 at position 469. cell culture grown prv 4f is unique in having a hydrophilic residue, glutamine, at amino acid 469; all previously sequenced vp4s have hydrophobi ... | 1994 | 8030238 |
| identification of bovine and porcine rotavirus g types by pcr. | a new seminested pcr typing assay has been extended to identify the important veterinary rotavirus serotypes g5, g6, g10, and g11, as well as the rare human serotype g8. the specificity of the method was evaluated with 30 standard laboratory strains of the g1 to g6 and g8 to g11 types. rotavirus strain types g6 and g8, not previously recognized in pigs, were identified in field specimens of porcine origin. | 1994 | 8051263 |
| sequence analysis of two porcine rotaviruses differing in growth in vitro and in pathogenicity: distinct vp4 sequences and conservation of ns53, vp6 and vp7 genes. | the vp4, vp7, ns53 and vp6 genes of two porcine rotavirus variants which differ in their in vitro growth properties and pathogenicity have been cloned and sequenced. the vp4 genes show only 67.2% nucleic acid and 70.6% amino acid identity. the vp4 gene of one variant (4s) is closely related to that of the bovine uk rotavirus strain, whereas the vp4 gene of the other variant (4f) is only distantly related to known vp4 genes and is likely to represent a new p serotype. in contrast the ns53 (vp5), ... | 1994 | 8077920 |
| mapping of antigenic sites involved in serotype-cross-reactive neutralization on group a rotavirus outercapsid glycoprotein vp7. | two neutralizing monoclonal antibodies (n-mabs) were utilized to locate amino acid (aa) residues involved in the formation of serotype-cross-reactive epitopes on the vp7 of selected group a rotaviruses. n-mab 954/159/13 neutralized g serotype 3 as well as porcine g serotype 4 rotaviruses, whereas n-mab 57/8 neutralized g serotype 3, 4, 6, 9, and 10 strains. neutralization-resistant variants of each serotype were selected in the presence of these two monoclonal antibodies. sequence analysis of th ... | 1994 | 8116249 |
| serological and genomic characterization of two porcine rotaviruses with serotype g1 specificity. | two porcine rotavirus strains, c60 and c95, which had been previously shown to be reactive in an enzyme-linked immunosorbent assay with serotype g1-specific monoclonal antibodies, were classified as g1 by cross-neutralization tests and on the basis of the homology of the sequenced vp7 gene. this report confirms that porcine rotavirus strains with a g1 serotype occur in nature. | 1994 | 8126197 |
| genomic relatedness of five equine rotavirus strains with different g serotype and p type specificities. | overall genomic relatedness among five equine rotavirus strains and their relatedness to representative human and animal rotavirus strains were investigated by rna-rna hybridization tests. the genomes of strains fi-14, fi-23 and h2 were highly related to one another. strain l338 had only a low degree of genomic relatedness to the other four equine rotavirus strains. strain h1 also showed little genetic relatedness to the other equine strains. the genome of the strain h1, however, was highly rela ... | 1993 | 8140288 |
| serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine vp4 and human and porcine vp7 genes. | of 216 fecal and intestinal samples collected from nursing or weaned diarrheic pigs in the united states and canada, 57 were identified as group a rotavirus positive by rna electrophoresis and silver staining. fifty-seven and 52 rotavirus-positive samples were analyzed by hybridization with gottfried and osu pcr-derived gene 9 and 4 probes, respectively. only 17 samples were identified with either homologous vp4 (p)- or vp7 (g)-coding genes or both. one rotavirus identified as g4 and p7 was simi ... | 1994 | 8150940 |
| a serologic survey of selected viral and bacterial diseases of european wild hogs, great smoky mountains national park, usa. | blood samples were collected from 108 wild hogs (sus scrofa) from the great smoky mountains national park (gsmnp), usa, february to july 1990. we found no antibodies for swine brucellosis, pseudorabies, bovine virus diarrhea virus or porcine rotavirus infection. antibody titers to porcine parvovirus were found in 15 (14%) samples and antibody to one or more leptospiral serovars was found in 48 (44%) samples. thirty-nine (89%) of the 44 positive samples reacted to all five leptospiral serovars te ... | 1994 | 8151810 |
| prevalence of p types among porcine rotaviruses using subgenomic vp4 gene probes. | nucleic acid probes were developed to differentiate vp4 (p) types among porcine rotaviruses. these probes were then used to determine the relative prevalence of p types 6 (gottfried-like) and 7 (osu-like) in cultivated rotaviruses and field specimens. the variable regions between bases 205-551 of the vp4 gene of rotavirus strains osu and gottfried were amplified by the polymerase chain reaction and radiolabeled with 32p by random primer extension. radiolabeled probes were tested in a dot blot hy ... | 1994 | 8203132 |
| assay for evaluation of rotavirus-cell interactions: identification of an enterocyte ganglioside fraction that mediates group a porcine rotavirus recognition. | a virus-host cell-binding assay was developed and used to investigate specific binding between group a porcine rotavirus and ma-104 cells or porcine enterocytes. a variety of glycoconjugates and cellular components were screened for their ability to block rotavirus binding to cells. during these experiments a crude ganglioside mixture was observed to specifically block rotavirus binding. on the basis of these results, enterocytes were harvested from susceptible piglets and a polar lipid fraction ... | 1994 | 8254737 |
| vp4 serotype of the gottfried strain of porcine rotavirus. | the antigenic relationships of the vp4 serotype of porcine rotavirus gottfried strain with other rotaviruses were determined by using antiserum to gottfried vp4-baculovirus recombinant. this antiserum failed to react significantly with virus of serotypes p1a, p1b, p3, and osu; however, it reacted with p2 strains. in the reciprocal assay, antiserum to vp4 of an asymptomatic strain (p2) failed to neutralize the gottfried strain virus to a significant level. it thus appears that the gottfried strai ... | 1993 | 8263206 |
| protein ns26 is highly conserved among porcine rotavirus strains. | 1993 | 8383837 | |
| sequence analysis of rotavirus ym vp6 and ns28 proteins. | we have determined the nucleotide sequence of genes 6 and 10 of porcine rotavirus ym. when the amino acid sequences of vp6 and ns28, the protein products of genes 6 and 10 respectively, were compared with other published sequences it was evident that the proteins of human rotavirus wa have the highest degree of identity with rotavirus ym. this is in contrast with the observation that when other proteins of these two strains have been compared they have been found to be among the most distantly r ... | 1993 | 8389808 |
| recovery and characterization of a rotavirus outer capsid protein expressed in a recombinant insect cell system. | recombinant osu vp4 protein, and outer capsid antigen of porcine rotavirus, was purified to a high level from the spent broth of baculovirus-infected spodoptera frugiperda insect cells. initial clarification of the broth with a 0-60% ammonium sulfate cut retained 93% of the total vp4. q-sepharose ion exchange chromatography performed at ph 6.5 yielded 67% of the initial amount of vp4 in the pooled fractions, with more than four times the purity of the original sample. gel filtration chromatograp ... | 1993 | 8390880 |
| reactivity of anti-human rotavirus vp4 neutralizing monoclonal antibodies with animal rotaviruses and with unusual human rotaviruses having different p and g serotypes. | the reactivity of five anti-human rotavirus vp4 neutralizing monoclonal antibodies (n-mab) with 20 animal rotavirus strains and three unusual human rotavirus (hrv) strains was investigated. five n-mab prepared previously by the immunization of mice with hrv were employed in this study. they were found to neutralize hrv belonging to p types 4, 6 and 8, which were designated according to p (or vp4) type nomenclature proposed by estes and cohen (1989). a porcine rotavirus strain gottfried was react ... | 1993 | 8395075 |
| [expression of a gene encoding the porcine rotavirus vp7 capsid using a nondefective recombinant adenoviral genome]. | a nondefective recombinant human adenovirus 5 (ad5) carrying the gene encoding for the porcine rotavirus outer capsid protein vp7 in the e3 region of the ad5 genome has been obtained. mrna for vp7 in recombinant ad5kv14/vp7 infected cells was demonstrated by means of rt-pcr. radioimmunoprecipitation of cell extracts infected with ad5kv14/vp7 on early (12 h p.i.) and late (24 h p.i.) stages of adenovirus infection with rabbit polyclonal antiserum raised to purified rotavirus virions revealed a re ... | 1995 | 8604234 |
| cross-reactive, serotype- and monotype-specific neutralization epitopes on vp7 of serotype g3 and g5 porcine rotavirus strains. | vp7 specific monoclonal antibodies raised against serotype g5 porcine rotavirus strains isolated in venezuela showed either a serotype g5- or monotype-specific pattern of reactivity by neutralization against a panel of 53 group a rotavirus isolates representative of all established g serotypes. monoclonal antibodies raised against two g3 porcine strains were either specific for a subset of porcine g3 strains or reactive with another subset of porcine g3 strains and with most g5 strains. neither ... | 1996 | 8645098 |
| serological and genomic characterization of porcine rotaviruses in thailand: detection of a g10 porcine rotavirus. | a total of 557 fecal specimens collected from piglets with diarrhea in thailand were examined for rotavirus rna by polyacrylamide gel electrophoresis. twenty-three, one, and two samples were positive for group a, group b, and group c rotaviruses, respectively. two samples exhibited two segments found in picobirnavirus rna. rna electropherotyping of 23 group a rotaviruses showed that they were classified into five patterns. by serotyping by enzyme-linked immunosorbent assay and pcr, viruses in 3 ... | 1996 | 8727874 |
| isolation of group b porcine rotavirus in cell culture. | while group a and c rotaviruses have been grown in cell culture, group b rotavirus has never been cultured. in this study we successfully isolated porcine group b rotavirus in swine kidney cells. pancreatin treatment is essential for the propagation of group b rotavirus. | 1996 | 8904456 |
| genetic stability of a porcine rotavirus rna segment during repeated plaque isolation. | the porcine group a rotavirus cc86 was characterized to explore its utility as a tool for mutation analysis. it has a semiduplication of the gene 11 rna segment. nucleotide sequence determination of cdna confirmed that the nsp5 coding sequence and the conserved nontranslated termini of the rna segment were retained. a comparison of the nsp5 genes of cc86 and cn86 that were isolated from the same fecal specimen showed eight base pair changes, suggesting that cn86 was not the immediate progenitor ... | 1996 | 8918545 |
| periplasmic expression of part of the major rotavirus capsid protein vp7 containing all the three antigenic regions in escherichia coli. | part of the porcine rotavirus outer capsid protein vp7 containing all the three antigenic regions was expressed as a chimeric protein with bacterial alkaline phosphatase (ap) in e. coli. the construct contains an ompf promoter, the dna encoding the signal sequence and the first 12 amino acids of mature ompf, part of vp7 and the dna encoding mature ap. the chimeric protein is stable, retains the biological property of ap and ability to react with polyclonal antiserum against the virus, and can be ... | 1996 | 8921861 |
| identification of a t-helper cell epitope on the rotavirus vp6 protein. | in this work, we have studied the t-helper (th)-cell response against rotavirus, in a mouse model. adult balb/c mice were inoculated parenterally with porcine rotavirus ym, and the th-cell response from spleen cells against the virus and two overlapping fragments of the major capsid protein vp6 (vp6(1-192) and vp6(171-397)) were evaluated in vitro. the th cells recognized the ym virus and the two protein fragments, suggesting that there are at least two th-cell epitopes on the vp6 molecule. to s ... | 1997 | 8985366 |
| viral determinants of rotavirus pathogenicity in pigs: production of reassortants by asynchronous coinfection. | a porcine rotavirus (prv), variant 4f, isolated in tissue culture from the feces of a chinese pig with diarrhea, was found to have become highly pathogenic when passaged in gnotobiotic piglets (j. c. bridger, b. burke, g. m. beards, and u. desselberger, j. gen. virol. 73:3011-3015, 1992). comparison with the closely related pig-apathogenic variant prv 4s suggested the outer capsid protein vp4 (encoded by rna 4) of prv 4f as a determinant for pathogenicity (b. burke, j. c. bridger, and u. desselb ... | 1997 | 8985430 |
| identification of the minimal replicase and the minimal promoter of (-)-strand synthesis, functional in rotavirus rna replication in vitro. | an in vitro replication system supporting the initiation and synthesis of complete rotavirus (-)-strands on (+)-strand template rna (chen et al., j virol 68: 7030, 1994) was used to examine several parameters related to rotavirus rna replication. coexpression of vp1/2/3 in all possible combinations from baculovirus vectors revealed: [i] virus-like particles (vlps) were formed only if vp2 was present, and [ii] vp1/2 and vp1/2/3 vlps had replicase activity in the in vitro system whereas vp2/3 and ... | 1996 | 9015102 |
| rapid diagnosis of porcine epidemic diarrhea virus infection by polymerase chain reaction. | the diagnosis of porcine epidemic diarrhea virus (pedv) infection in the laboratory is rather fastidious because of difficulties in virus propagation. the feasibility of virus propagation in vivo is also limited by the handling of a number of samples at the same time. in this study, the detection of pedv by reverse transcription polymerase chain reaction (rt-pcr) is described. the rt-pcr could detect up to 10(4) tcid50/ml of pedv and did not show any cross reaction with transmissible gastroenter ... | 1997 | 9101487 |
| characterization of a porcine enterocyte receptor for group a rotavirus. | we have identified, purified to apparent homogeneity and chemically characterized a biologically-relevant porcine enterocyte receptor for group a porcine rotavirus. ceramide glycanase digestion followed by acid hydrolysis and monosaccharide compositional analyses indicated the receptor is a family of two gm, gangliosides, one containing n-glycolyl-neuraminic acid and the other n-acetylneuraminic acid. both gangliosides displayed dose-dependent inhibition of rotavirus binding to, and infectivity ... | 1997 | 9192005 |
| single point mutations may affect the serotype reactivity of serotype g11 porcine rotavirus strains: a widening spectrum? | a panel of single and double neutralization-resistant escape mutants of serotype g11 porcine rotavirus strains a253 and ym, selected with g11 monotype- and serotype-specific neutralizing monoclonal antibodies (mabs) to vp7, was tested in neutralization assays with hyperimmune sera raised against rotavirus strains of different serotypes. escape mutants with an amino acid substitution in antigenic region a (amino acids [aa] 87 to 101) resulting in a residue identical or chemically similar to those ... | 1997 | 9343172 |
| characterization of human rotavirus genotype p[8]g5 from brazil by probe-hybridization and sequence. | we report the molecular characterization of rotavirus genotype p[8]g5 strains found in fecal specimens collected in four different regions of brazil, using digoxigenin(dig)-labeled oligonucleotide probes, sequence analysis, and rna-rna hybridization. the closest sequence relationships of the neutralization antigens of these strains were to the vp4 protein of p1a[8]g1 strain ku (93.3% identity in amino acids 11 to 282) and to the vp7 protein of g serotype 5 strain osu (87.6% identity in amino aci ... | 1996 | 9526542 |
| determinants of rotavirus host range restriction--a heterologous bovine nsp1 gene does not affect replication kinetics in the pig. | the genetic basis of rotavirus host range restriction (host species specificity) is unknown but the nsp1 (fifth) gene has been implicated in some studies. we studied the replication kinetics in vivo of a nsp1 gene monoreassortant, e11, to assess the influence of a heterologous nsp1 gene on the ability to replicate in pigs. the monoreassortant possessed 10 genes from the porcine parent rotavirus sw20/21, which replicated productively in pigs, and the nsp1 gene from the bovine rotavirus uk which p ... | 1998 | 9614866 |
| viral determinants of rotavirus pathogenicity in pigs: evidence that the fourth gene of a porcine rotavirus confers diarrhea in the homologous host. | a porcine rotavirus (prv) monoreassortant, s-f4, which carries rna segment 4 of the pig-pathogenic variant prv 4f in the genetic background of the pig-apathogenic variant prv 4s (g. i. tauscher and u. desselberger, j. virol. 71:853-857, 1997), was found to be pathogenic in gnotobiotic piglets. this indicates that rna segment 4 of the pig-pathogenic variant prv 4f is a major determinant of pathogenicity in its homologous host. | 1998 | 9658148 |
| structure and function of a ganglioside receptor for porcine rotavirus. | a ganglioside fraction isolated from pooled intestines from newborn to 4-week-old piglets, which we previously partially characterized and showed to specifically inhibit the binding of porcine rotavirus (osu strain) to host cells (m. d. rolsma, h. b. gelberg, and m. s. kuhlenschmidt, j. virol. 68:258-268, 1994), was further purified and found to contain two major monosialogangliosides. each ganglioside was purified to apparent homogeneity, and their carbohydrate structure was examined by high-ph ... | 1998 | 9765453 |
| adaptation and serial passage of bovine coronavirus in an established diploid swine testicular cell line and subsequent development of a modified live vaccine. | a virulent bovine coronavirus isolate (newborn calf diarrheal) was adapted and serially passaged in an established diploid swine testicular cell line (st cells). the same cells have been used to produce modified live porcine rotavirus and coronavirus vaccines that are federally licensed and sold worldwide. growth of the bovine coronavirus resulted in cytopathic effect characterized by cellular stranding and subsequent cell lysis. virus yields were relatively high in the st cells and active repli ... | 1998 | 9782348 |
| isolation of a human rotavirus strain with a super-short rna pattern and a new p2 subtype. | super-short rotavirus strains that have a rearranged gene segment 11 are rarely found in humans, and only five isolates, all from southeast asia, have been described in the literature. we report the first isolation in japan from an infant with severe diarrhea of a rotavirus possessing a super-short rna pattern. this strain, designated au19, had a g1 vp7 and is also the first isolate in japan that possesses a p2[6] vp4. furthermore, the p2[6] vp4 carried by au19 was divergent in the hypervariable ... | 1999 | 10074557 |
| identification of two lineages (wa-like and f45-like) within the major rotavirus genotype p[8]. | the fourth gene of a porcine (s8) and eight human rotavirus isolates possessing the major human vp4 specificity (p1a serotype and/or p[8] genotype) were partially sequenced and compared to other available p[8] sequences from rotaviruses types g1, g3, g5 and g9 specificities which had been originally recovered from children with diarrhea in japan, brazil and the usa. brazilian rotavirus s8 represented the single known porcine rotavirus with this p specificity. phylogenetic analysis revealed two l ... | 1999 | 10082386 |
| detection of porcine rotavirus type g9 and of a mixture of types g1 and g5 associated with wa-like vp4 specificity: evidence for natural human-porcine genetic reassortment. | rotavirus type g5 is a primarily porcine pathogen that has caused frequent and widespread diarrhea in children in brazil and in piglets elsewhere. initial results on the rotavirus types circulating in diarrheic piglets in brazil disclosed a high diversity of strains with distinct g types including g1, g4, g5, and g9 and the novelty of p[8], the predominant human p specificity type. those results add strong evidence for the emergence of new strains through natural reassortment between rotaviruses ... | 1999 | 10405435 |
| bacterial expression of the major antigenic regions of porcine rotavirus vp7 induces a neutralizing immune response in mice. | the outer capsid protein of rotavirus, vp7, is a major neutralization antigen. a chimeric protein comprising escherichia coli (e. coli) outer membrane protein a (ompa) and part of porcine rotavirus vp7 containing all three antigenic regions (217 amino acids) was expressed in salmonella and e. coli as an outer-membrane associated protein. mice immunized intraperitoneally or orally, respectively, with live e. coli or salmonella cells expressing this chimeric protein produced antibodies against nat ... | 1999 | 10418913 |
| diarrhea induction by rotavirus nsp4 in the homologous mouse model system. | comparison of the nsp4 amino acid sequences from 31 strains of mammalian rotaviruses revealed the presence of four distinct nsp4 alleles; i.e., the wa, kun, au-1, and ew alleles. the ew allele consists only of nsp4s from murine rotavirus strains and is divergent from other nsp4 alleles from the evolutionary perspective. there have been conflicting reports regarding the enterotoxigenic activity of nsp4 in the mouse model system; heterologous simian and porcine rotavirus nsp4s function as an enter ... | 1999 | 10502518 |
| entry of rotaviruses is a multistep process. | the infection of epithelial cells by some animal rotavirus strains requires the presence of sialic acid (sa) on the cell surface. recently, we isolated rhesus rotavirus variants, named nar, whose infectivity, like that of human rotaviruses, is not dependent on sa. in this work, we have determined the binding properties of these sa-dependent and -independent rotavirus strains to ma104 cells. the half-time of attachment of the sa-dependent porcine rotavirus ym and reassortant virus ds1xrrv was fou ... | 1999 | 10544117 |
| new p serotype of group a human rotavirus closely related to that of a porcine rotavirus. | the vp7 and vp4 genes of two human group a rotavirus strains mc323 and mc345 with unique serologic and genomic properties, and isolated in chiang mai, thailand, in 1989 [urasawa et al. (1992) journal of infectious diseases 166:227-234] were further characterized. the nucleotide and deduced amino acid sequences of the vp7 genes allowed the classification of both strains as serotype g9. the vp4 genes of both strains are 2,359 nucleotides in length and encode a protein of 775 amino acids like in mo ... | 2000 | 10568765 |
| comparison of polymerase chain reaction and dot hybridization with enzyme-linked immunoassay, virological examination and polyacrylamide gel electrophoresis for the detection of porcine rotavirus in faecal specimens. | the purpose of this study was the evaluation of rt-pcr (reverse transcription-polymerase chain reaction) technique and dot blot hybridization with pcr-generated probe for the detection of group a rotavirus in faecal samples derived from diarrhoeal piglets. they were compared with virological examination (isolation of the virus), polyacrylamide gel electrophoresis (page) and elisa. the specificity and sensitivity of each assay was assessed against a 'gold standard' which was created on the basis ... | 1999 | 10605372 |
| sialic acid dependence and independence of group a rotaviruses. | we have found (1), in contrast to previous reports, the human rotavirus wa strain is sialic acid-dependent for binding to and infectivity of ma-104 cells and (2), a dual carbohydrate binding specificity is associated with both human wa and porcine osu rotaviruses. one carbohydrate binding activity is associated with triple-layered virus particles (tlp) and the other with double-layered virus particles (dlp). in binding and infectivity studies, we found that gangliosides were the most potent inhi ... | 1999 | 10659372 |
| the stability of porcine rotavirus in feces. | rotaviruses are known as major causal agents of diarrhea in humans and animals. they affect young animals in intensive rearing and cause great economic losses. this study evaluated the infectivity of porcine rotavirus maintained for 32 months at approximately 10 degrees c in the original stool specimens. thirty stool specimens of 1-4-week-old piglets from breeding farms located in the southwest of the state of parana were selected for this study. they were randomly chosen from stool samples posi ... | 2000 | 10665529 |
| rotavirus vp7 epitope mapping using fragments of vp7 displayed on phages. | cdna copies of the complete porcine rotavirus crw-8 vp7 gene were randomly digested to fragments of about 30-60 or 30-500 base pairs by dnase1 in the presence of mn(2+). the fragments were cloned and expressed in a filamentous phage fd-tet-derived vector to create specific-gene-related peptide libraries. polyclonal antibodies were then used to pan the sgrp libraries for antibody-binding phages. analysis of the phage isolates revealed that the majority (86%) of them only had a single insert. howe ... | 2000 | 10717346 |
| antigenic and molecular analyses reveal that the equine rotavirus strain h-1 is closely related to porcine, but not equine, rotaviruses: interspecies transmission from pigs to horses? | we have sequenced the genes encoding the inner capsid protein vp6 and the outer capsid glycoprotein vp7 of the subgroup (sg) i equine rotavirus strain h-1 (p9[7], g5). the vp6 and vp7 proteins of the equine rotavirus strain h-1 shared a high degree of sequence and deduced amino acid identity with sg i porcine strains and serotype g5 porcine strains, respectively. previous sequence analyses of the genes encoding the outer capsid spike protein vp4 and the nonstructural proteins nsp1 and nsp4 of eq ... | 2001 | 11210939 |
| selection of rotavirus vp4 cell receptor binding domains for ma104 cells using a phage display library. | rotavirus infection of host cells, like other viruses, is a complex process that has not been fully elucidated, and much attention has been focused on the regions of the viral attachment protein, vp4, that are involved in binding to the cellular receptor. in this study, phage display technology was employed to generate a g3p vp4 gene-targeted phage display peptide library using the porcine rotavirus strain crw8, and a method was optimised for panning this library on adherent ma104 cells to ident ... | 2001 | 11543883 |
| non-lytic extraction and characterisation of receptors for multiple strains of rotavirus. | to characterise the cellular receptors for rotavirus, we used the detergent octyl-beta-d-glucopyranoside (octyl-glucoside/og) to extract the receptors for bovine, simian, porcine and human rotaviruses from ma104 and ht29 cells. an octyl-glucoside concentration of 0.2% dramatically reduced the susceptibility of treated cells to infection, while leaving them metabolically active, and as a result the depleted receptors were able to regenerate. periodate treatment of the ma104 and ht29 octyl-glucosi ... | 2001 | 11556708 |
| nested reverse transcriptase-polymerase chain reaction for the detection of group a rotaviruses. | rotaviruses are important pathogens associated with diarrhoeal diseases in almost all species of mammals. in the present study, a nested reverse transcriptase-polymerase chain reaction (rt-pcr) for the detection of group a rotaviruses was developed, which is based on a target region in gene segment 6. rotavirus strains of human, bovine, porcine, canine, feline, equine, and ovine origin were examined. furthermore several faecal specimens, in which rotavirus had already been detected using other m ... | 2002 | 12002423 |
| survey of porcine rotavirus g and p genotype in poland and the united states using rt-pcr. | porcine rotaviruses are a common cause of gastroenteritis. several serotypes have been detected based on the two surface proteins vp4 (p-types) and vp7 (g-types). however, limited studies have been performed on the relative frequency of rotavirus types in diarrhetic pigs primarily because of the lack of availability of suitable methods. in this study, we describe a reverse transcriptase-polymerase chain reaction (rt-pcr) method for the typing of p and g types of rotavirus. this method allowed to ... | 2002 | 12449245 |
| mucosal immune responses following oral immunization with rotavirus antigens encapsulated in alginate microspheres. | availability of effective oral vaccine delivery vehicles should contribute to the success of oral immunization in domestic animals. to achieve this goal, we evaluated alginate microspheres for their capacity to induce mucosal immune responses following oral and enteric immunizations. mice were immunized with either live porcine rotavirus (prv) or its recombinant vp6 protein, encapsulated in alginate microspheres or unencapsulated. vp6-specific igg (but no iga) antibodies were detected in the ser ... | 2002 | 12480324 |
| relationship between group a porcine rotavirus and management practices in swine herds in ontario. | a case-control epidemiological study was conducted to determine whether an increased diagnostic rate for group a rotavirus in swine herds in ontario was associated with specific management factors. the number of new herds tested per year and the proportion of new positive herds increased between 1994 and 1997. herd size was larger and weaning age was younger in rotavirus-positive herds compared with rotavirus-negative herds. pigs raised in all-in all-out nurseries were 3.4 times more likely to h ... | 2003 | 13677596 |
| a novel type of vp4 carried by a porcine rotavirus strain. | the gene encoding the vp8* trypsin-cleavage product of the vp4 protein of porcine rotavirus strain a34 was sequenced, and the predicted amino acid (aa) sequence was compared to the homologous region of all known p genotypes. the aa sequence of the vp8* of strain a34 shared low identity, ranging from 39% (bovine strain b223, p8[11]) to 76% (human strain 69m, p4[10]), with the homologous sequences of representative strains of the remaining 21 p genotypes. phylogenetic relationships showed that the ... | 2003 | 14585340 |
| species-specific but not genotype-specific primary and secondary isotype-specific nsp4 antibody responses in gnotobiotic calves and piglets infected with homologous host bovine (nsp4[a]) or porcine (nsp4[b]) rotavirus. | using recombinant baculoviruses expressing rotavirus nsp4 [a], [b], [c], and [d] genotypes of bovine, porcine, human, simian, or murine origin, we analyzed serum antibody responses to nsp4s in gnotobiotic calves and piglets infected by the oral/alimentary or intraamniotic route with bovine (nsp4[a]) (wyatt, r.g., mebus, c.a., yolken, r.h., kalica, a.r., james, h.d., jr., kapikian, a.z., chanock, r.m., 1979. rotaviral immunity in gnotobiotic calves: heterologous resistance to human virus induced ... | 2004 | 15527837 |
| a porcine g9 rotavirus strain shares neutralization and vp7 phylogenetic sequence lineage 3 characteristics with contemporary human g9 rotavirus strains. | of five globally important vp7 (g) serotypes (g1-4 and 9) of group a rotaviruses (the single most important etiologic agents of infantile diarrhea worldwide), g9 continues to attract considerable attention because of its unique natural history. serotype g9 rotavirus was isolated from a child with diarrhea first in the united states in 1983 and subsequently in japan in 1985. curiously, soon after their detection, g9 rotaviruses were not detected for about a decade in both countries and then reeme ... | 2005 | 15661150 |
| predominance of porcine rotavirus g9 in japanese piglets with diarrhea: close relationship of their vp7 genes with those of recent human g9 strains. | type g9 of group a rotavirus (gar) was shown to be predominant in a survey of vp7 (g) and vp4 (p) genotypes among porcine gars associated with outbreaks of diarrhea in young pigs in japan between 2000 and 2002. comparison of the g9 vp7 gene sequences showed that the porcine g9 strains were more closely related to human g9 strains reemerging globally since the mid-1990s than to those from the mid-1980s. the vp7 gene sequences of porcine g9 strains from different farms were divergent (6.1 to 7.2% ... | 2005 | 15750112 |
| porcine rotavirus strain gottfried-based human rotavirus candidate vaccines: construction and characterization. | rotavirus gastroenteritis remains the leading cause of severe diarrheal disease in infants and young children worldwide, and thus, a safe and effective rotavirus vaccine is urgently needed in both developing and developed countries. various candidate rotavirus vaccines that were developed by us and others have been or are being evaluated in different populations in various parts of the world. we have recently confirmed that a porcine rotavirus gottfried strain bears a p (vp4) serotype (p2b[6]) c ... | 2005 | 15893616 |
| characterization of neuraminidase-resistant mutants derived from rotavirus porcine strain osu. | infection by some rotavirus strains requires the presence of sialic acid on the cell surface, its infectivity being reduced in cells treated with neuraminidase. a neuraminidase treatment-resistant mutant was isolated from the porcine rotavirus strain osu. in reassortant strains, the neuraminidase-resistant phenotype segregated with the gene coding for vp4. the mutant retained its capacity to bind to sialic acid. the vp4 sequence of the mutant differed from that of the parental osu strain in an a ... | 2005 | 16051829 |
| evaluation of specificity and effects of monoclonal antibodies submitted to the eighth human leucocyte differentiation antigen workshop on rotavirus-cell attachment and entry. | rotavirus infection of permissive cells is a multi-step process that requires interaction with several cell surface receptors. integrins alpha2beta1, alpha4beta1, alphaxbeta2, and alphavbeta3 are involved in the attachment and entry into permissive cells for many rotavirus strains. however, possible roles of known partners of these integrins in this process have not been studied. here, the specificities of new monoclonal antibodies directed to beta1 and beta2 integrins were determined using inte ... | 2005 | 16169540 |
| relationships among porcine and human p[6] rotaviruses: evidence that the different human p[6] lineages have originated from multiple interspecies transmission events. | porcine rotavirus strains (porvs) bearing human-like vp4 p[6] gene alleles were identified. genetic characterization with either pcr genotyping or sequence analysis allowed to determine the vp7 specificity of the porvs as g3, g4, g5 and g9, and the vp6 as genogroup i, that is predictive of a subgroup i specificity. sequence analysis of the vp8* trypsin-cleavage product of vp4 allowed porvs to be characterized further into genetic lineages within the p[6] genotype. unexpectedly, the strains displ ... | 2006 | 16194556 |
| rotaviruses interact with alpha4beta7 and alpha4beta1 integrins by binding the same integrin domains as natural ligands. | group a rotaviruses are major intestinal pathogens that express potential alpha4beta1 and alpha4beta7 integrin ligand sequences leu-asp-val and leu-asp-ile in their outer capsid protein vp7, and ile-asp-ala in their spike protein vp4. monkey rotavirus sa11 can use recombinant alpha4beta1 as a cellular receptor. in this study a new potential alpha4beta1, alpha4beta7 and alpha9beta1 integrin ligand sequence, tyr-gly-leu, was identified in vp4. it was shown that several human and monkey rotaviruses ... | 2005 | 16298987 |
| [expression of recombinant vp6 gene of porcine rotavirus a with non-antibiotic lactobacillus vector in escherichia coli]. | the antigenic determinants of vp6 gene of porcine rotavirus a was amplified from infected ma 104 cell by the reverse transcription-polymerase chain reaction (rt-pcr), the product of which was a 1194bp cdna segment. using t-a cloning technique, the pcr product was cloned into pgem-t vector. cloning plasmid pgem-t-vp6 and the prokaryotic shuttle expression vector pw425t between e. coli and lactobacillus, were digested by saci and kpni double enzymes, respectively. the purified vp6 gene was subclon ... | 2005 | 16342756 |
| identification of a novel vp4 genotype carried by a serotype g5 porcine rotavirus strain. | rotavirus genome segment 4, encoding the spike outer capsid vp4 protein, of a porcine rotavirus (porv) strain, 134/04-15, identified in italy was sequenced, and the predicted amino acid (aa) sequence was compared to those of all known vp4 (p) genotypes. the aa sequence of the full-length vp4 protein of the porv strain 134/04-15 showed aa identity values ranging from 59.7% (bovine strain kk3, p8[11]) to 86.09% (porcine strain a46, p[13]) with those of the remaining 25 p genotypes. moreover, aa se ... | 2006 | 16364388 |
| crystallization and preliminary x-ray diffraction analysis of the sialic acid-binding domain (vp8*) of porcine rotavirus strain crw-8. | rotavirus recognition and attachment to host cells involves interaction with the spike protein vp4 that projects outwards from the surface of the virus particle. an integral component of these spikes is the vp8* domain, which is implicated in the direct recognition and binding of sialic acid-containing cell-surface carbohydrates and facilitates subsequent invasion by the virus. the expression, purification, crystallization and preliminary x-ray diffraction analysis of vp8* from porcine crw-8 rot ... | 2005 | 16511112 |
| rotavirus spike protein vp5* binds alpha2beta1 integrin on the cell surface and competes with virus for cell binding and infectivity. | rotaviruses recognize several cell-surface molecules, including the alpha2beta1 integrin, and the processes of rotavirus cell attachment and entry appear to be multifactorial. the vp5* subunit of the rotavirus spike protein vp4 contains the alpha2beta1 ligand sequence asp-gly-glu at residues 308-310. binding to alpha2beta1 and infectivity of monkey rotavirus strain rrv and human rotavirus strain wa, but not porcine rotavirus strain crw-8, are inhibited by peptides containing asp-gly-glu. asp308 ... | 2006 | 16603530 |
| characterization of vp1, vp2 and vp3 gene segments of a human rotavirus closely related to porcine strains. | long rna electropherotype rotavirus strains with subgroup i specificity predominated the infantile gastroenteritis outbreak in manipur, india, in 1987-88. one such strain (rmc321) was found to possess porcine characteristics in 7 out of 8 genes sequenced. partial characterization of its remaining vp1, vp2 and vp3 genes along with a porcine rotavirus strain (hp140) uncovered their close genetic relation to porcine strains. vp7 was the only gene segment of this strain with significant genetic iden ... | 2006 | 16732476 |
| detection of rare g3p[19] porcine rotavirus strains in chiang mai, thailand, provides evidence for origin of the vp4 genes of mc323 and mc345 human rotaviruses. | among 175 fecal specimens collected from diarrheic piglets during a surveillance of porcine rotavirus (porv) strains in chiang mai, thailand, 39 (22.3%) were positive for group a rotaviruses. of these, 33.3% (13 of 39) belonged to g3p[19], which was a rare p genotype seldom reported. interestingly, their vp4 nucleotide sequences were most closely related to human p[19] strains (mc323 and mc345) isolated in 1989 from the same geographical area where these porv strains were isolated. these p[19] p ... | 2006 | 16988014 |
| molecular characterization of a new porcine rotavirus p genotype found in an asymptomatic pig in slovenia. | rotaviral rna was detected in the stool sample of an asymptomatic fattening pig at a slovenian pig farm. to characterize the rotavirus, rt-pcr was used, employing primers specific for the vp7, vp4 and nsp4 genes. specific products were purified and the sequencing reaction was performed for the molecular analysis of amplified genes. nucleotide and amino acid sequences of the vp7 gene were found highly identical (85.3-88.1% and 90.7-91.6%) to g1 genotype strains. phylogenetic and molecular analyse ... | 2007 | 17078990 |
| identification of group a porcine rotavirus strains bearing a novel vp4 (p) genotype in italian swine herds. | the vp4 gene of a g5 italian porcine rotavirus strain, 344/04-1, was nontypeable by pcr genotyping. the amino acid sequence of the full-length vp4 protein had low identity (<or=76.6%) with the homologous sequences of representative strains of the remaining p genotypes, providing evidence for a novel p genotype. | 2007 | 17122003 |
| intestinal ribosomal p70(s6k) signaling is increased in piglet rotavirus enteritis. | recent identification of the mammalian target of rapamycin (mtor) pathway as an amino acid-sensing mechanism that regulates protein synthesis led us to investigate its role in rotavirus diarrhea. we hypothesized that malnutrition would reduce the jejunal protein synthetic rate and mtor signaling via its target, ribosomal p70 s6 kinase (p70(s6k)). newborn piglets were artificially fed from birth and infected with porcine rotavirus on day 5 of life. study groups included infected (fully fed and 50 ... | 2007 | 17138969 |
| novel porcine rotavirus of genotype p[27] shares new phylogenetic lineage with g2 porcine rotavirus strain. | a novel and unusual strain of porcine rotavirus (porv) cmp034 was isolated from a 7-week-old piglet during the epidemiological survey of porcine rotavirus infection in chiang mai province, thailand from june 2000 to july 2001. molecular characterization of gene vp4 by sequence analysis showed a low level of amino acid sequence identity, ranging from 56.7% to 76.6%, while comparison of vp8* portion showed 41.8% to 69.9% identity, with the 26 p genotypes recognized to date. phylogenetic analysis o ... | 2007 | 17215015 |
| zinc-binding domain of rotavirus nsp1 is required for proteasome-dependent degradation of irf3 and autoregulatory nsp1 stability. | interferon regulatory factor 3 (irf3) is a key transcription factor involved in the induction of interferon (ifn) in response to viral infection. rotavirus non-structural protein nsp1 binds to and targets irf3 for proteasome degradation early post-infection. mutational analysis of cysteine and histidine residues within the conserved n-terminal zinc-binding domain in nsp1 of bovine rotavirus strain b641 abolished irf3 degradation in transfected cells. thus, the integrity of the zinc-binding domai ... | 2007 | 17251580 |
| expression, purification, crystallization and preliminary x-ray diffraction analysis of the vp8* sialic acid-binding domain of porcine rotavirus strain osu. | the rotavirus outer capsid spike protein vp4 is utilized in the process of rotavirus attachment to and membrane penetration of host cells. vp4 is cleaved by trypsin into two domains: vp8* and vp5*. the vp8* domain is implicated in initial interaction with sialic acid-containing cell-surface carbohydrates and triggers subsequent virus invasion. the vp8* domain from porcine osu rotavirus was cloned and expressed in escherichia coli. different crystal forms (orthorhombic p2(1)2(1)2(1) and tetragona ... | 2007 | 17277447 |
| insight into host cell carbohydrate-recognition by human and porcine rotavirus from crystal structures of the virion spike associated carbohydrate-binding domain (vp8*). | rotavirus infection leads to the death of half a million children annually. the exact specifics of interaction between rotavirus particles and host cells enabling invasion and infection have remained elusive. host cell oligosaccharides are critical components, and their involvement aids the virus in cell-recognition and attachment, as well as dictation of the remarkable host-specificity that rotaviruses demonstrate. interaction between the rotavirus spike-protein carbohydrate-binding domain (vp8 ... | 2007 | 17306299 |
| detection of a neonatal human rotavirus strain with vp4 and nsp4 genes of porcine origin. | a human rotavirus strain (nb-150) was detected in stool samples from a neonate hospitalized for mild/moderate community-acquired diarrhoea. this baby lived in the outskirts of belém, brazil, under poor sanitation conditions. the nb-150 strain displayed a typical long electrophoretic pattern with 11 gene segments. it had two vp7 alleles, g1 and g4, and belonged to vp6 subgroup ii. a close relatedness with human rotaviruses was shown for vp7 alleles: g1 (96.9-100 % similarity at the amino acid lev ... | 2007 | 17374895 |
| development of a simple and rapid immunochromatographic strip test for diarrhea-causative porcine rotavirus in swine stool. | a rapid and simple immunochromatography (ic) strip test, for specific detection of porcine rotavirus (prv) in stool specimen, was developed. monoclonal antibodies (mabs) to the osu strain of prv have been produced in mice. among them, two hybridoma clones that generate mab-1 and mab-2, respectively, specific for vp6 protein of prv, have been selected. in the ic configuration, mab-1, one of the selected mabs was used to the designed coat microparticles (mp), while another mab-2 was used to fix it ... | 2007 | 17644198 |
| phylogenetic analysis of porcine rotavirus in argentina: increasing diversity of g4 strains and evidence of interspecies transmission. | group a rotaviruses are one of the most frequently detected viral agents associated with neonatal diarrhea in piglets. in order to characterize rotavirus (rv) strains circulating in argentinean swine, four porcine production farms located in buenos aires were studied. rv strains genotyped as p[6]g4, p[6]g8 and p[1]g6 were found in piglets under 30 days of age, without diarrhea. phylogenetic and sequence analysis of the vp7 gene from g4 strains available in databases, reveals five porcine new lin ... | 2008 | 17659850 |
| impact of porcine group a rotavirus co-infection on porcine epidemic diarrhea virus pathogenicity in piglets. | porcine epidemic diarrhea virus (pedv) and porcine group a rotavirus (pgar) are the main causative agents of acute diarrhea in piglets. in south korea, pgar is prevalent in piglets naturally infected with pedv. piglets naturally co-infected with pedv and pgar appeared to have severe and prolonged diarrhea that was distinct from that commonly observed. the aim of this study was to determine the impact of pgar co-infection on pedv pathogenicity in piglets. thirty-six colostrum-deprived, one-day ol ... | 2008 | 17727905 |
| university-industry programs. | in the report by r. g. wyatt et al. "human rotavirus type 2: cultivation in vitro" (11 jan., p. 189), in the sentence describing the porcine rotavirus plaque reduction test (p. 190, column 3, line 16), the concentration of pancreatin in the agar overlay should have been given as "0.15 percent of 2.5 percen pantreatin 4 x n. f.; gibco." | 1980 | 17729852 |
| genetic relationship between porcine rotavirus strains bearing a new p-type. | 2007 | 17766061 | |
| sequence analysis of vietnamese p[6] rotavirus strains suggests evidence of interspecies transmission. | nucleotide and amino acid sequences of the vp8* gene of five vietnamese p[6] rotavirus strains detected from hospitalized patients with acute gastroenteritis were analyzed and compared with other human and porcine p[6] rotaviruses. it is of interest that these strains had greatest identity with two italian porcine rotavirus strains, 134/04-10 and 134/04-11. to our knowledge, these five vietnamese rotaviruses are the rare p[6] rotavirus strains belonging to lineage i that cluster into sublineage ... | 2007 | 17935191 |
| rotavirus g5p[6] in child with diarrhea, vietnam. | we detected rotavirus g5p[6] with a long rna pattern in a vietnamese child with diarrhea. viral outer capsid protein vp7 and vp4 genes suggest that it likely originated from porcine rotavirus either by genetic reassortment or as whole virions. to our knowledge, this is the first report of human rotavirus g5 in asia. | 2007 | 17953100 |
| arginine activates intestinal p70(s6k) and protein synthesis in piglet rotavirus enteritis. | we previously showed that phosphorylation of p70 s6 kinase (p70(s6k)) in the intestine is increased during viral enteritis. in this study, we hypothesized that during rotavirus infection, oral arg, which stimulates p70(s6k) activation, will further stimulate intestinal protein synthesis and mucosal recovery, whereas the p70(s6k) inhibitor rapamycin (rapa) will inhibit mucosal recovery. newborn piglets were fed a standard milk replacer diet supplemented with arg (0.4 g x kg(-1) x d(-1), twice dai ... | 2008 | 18156399 |