Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
an update on canine adenovirus type 2 and its vectors.adenovirus vectors have significant potential for long- or short-term gene transfer. preclinical and clinical studies using human derived adenoviruses (had) have demonstrated the feasibility of flexible hybrid vector designs, robust expression and induction of protective immunity. however, clinical use of had vectors can, under some conditions, be limited by pre-existing vector immunity. pre-existing humoral and cellular anti-capsid immunity limits the efficacy and duration of transgene expressi ...201021994722
3d cultures of human neural progenitor cells: dopaminergic differentiation and genetic modification. [corrected].central nervous system (cns) disorders remain a formidable challenge for the development of efficient therapies. cell and gene therapy approaches are promising alternatives that can have a tremendous impact by treating the causes of the disease rather than the symptoms, providing specific targeting and prolonged duration of action. hampering translation of gene-based therapeutic treatments of neurodegenerative diseases from experimental to clinical gene therapy is the lack of valid and reliable ...201222433395
a siglec-like sialic-acid-binding motif revealed in an adenovirus capsid protein.sialic-acid-binding immunoglobulin-like lectins (siglecs) are a family of transmembrane receptors that are well documented to play roles in regulation of innate and adaptive immune responses. to see whether the features that define the molecular recognition of sialic acid were found in other sialic-acid-binding proteins, we analyzed 127 structures with bound sialic acids found in the protein data bank database. of these, the canine adenovirus 2-fiber knob protein showed close local structural re ...201222522600
a novel recombinant peste des petits ruminants-canine adenovirus vaccine elicits long-lasting neutralizing antibody response against ppr in goats.peste des petits ruminants (ppr) is a highly contagious infectious disease of goats, sheep and small wild ruminant species with high morbidity and mortality rates. the peste des petits ruminants virus (pprv) expresses a hemagglutinin (h) glycoprotein on its outer envelope that is crucial for viral attachment to host cells and represents a key antigen for inducing the host immune response.201222623990
bioprocess development for canine adenovirus type 2 vectors.canine adenovirus type 2 (cav-2) vectors overcome many of the clinical immunogenic concerns related to vectors derived from human adenoviruses (advs). in addition, cav-2 vectors preferentially transduce neurons with an efficient traffic via axons to afferent regions when injected into the brain. to meet the need for preclinical and possibly clinical uses, scalable and robust production processes are required. cav-2 vectors are currently produced in e1-transcomplementing dog kidney (dk) cells, wh ...201322763405
hemorrhagic and necrotizing hepatitis associated with administration of a modified live canine adenovirus-2 vaccine in a maned wolf (chrysocyon brachyurus).a 15-yr-old, female, maned wolf (chrysocyon brachyurus) was euthanized after presenting semicomatose with severe, uncontrolled frank hemorrhage from her rectum 6 days following a routine physical examination and vaccination. histopathology indicated severe hemorrhagic and necrotizing hepatitis with intranuclear basophilic inclusion bodies in the liver that were thought to be consistent with adenoviral infection. further classification by polymerase chain reaction, immunohistochemical staining, v ...201222779244
pathogens of wild maned wolves (chrysocyon brachyurus) in brazil.the maned wolf, chrysocyon brachyurus, is an endangered neotropical canid that survives at low population densities. diseases are a potential threat for its conservation but to date have been poorly studied. we performed clinical evaluations and investigated the presence of infectious diseases through serology and coprologic tests on maned wolves from galheiro natural private reserve, perdizes city, minas gerais state, southeastern brazil. fifteen wolves were captured between 2003 and 2008. we f ...201223060508
duration of serological response to canine parvovirus-type 2, canine distemper virus, canine adenovirus type 1 and canine parainfluenza virus in client-owned dogs in australia.to determine whether client-owned dogs in australia, last vaccinated with canvac(®) vaccines containing canine parvovirus-type 2 (cpv-2), canine distemper virus (cdv), canine adenovirus type 2 (cav-2) ± canine parainfluenza virus (cpiv) at least 18 months ago, were seropositive or responded serologically to revaccination.201223186088
molecular analysis of a fragment of gene e1b 19k of canine adenovirus 2 (cav-2) isolated from dogs with symptoms of cough.the aim of this study was to perform molecular analysis of canine adenovirus 2 (cav-2) e1b 19k gene fragment isolated from 20 dogs of various breeds (12 males and 8 females aged 1-9 years), with clinical symptoms of upper respiratory tract infections, from the lubelszczyzna region. nasal swabs were taken from dogs. dna of cav-2 was detected using the pcr method in 16 swabs. all pcr products were sequenced, and the obtained sequences were compared with each other and with the sequence of the e1b ...201223214360
transducing properties of a pre-structured α-helical dpt-peptide containing a short canine adenovirus type 2 e4orf4 pp2a1-binding sequence.induction of the death pathway resulting from the specific interaction of the pp2a1 phosphatase with adenoviral e4orf4 protein is a promising approach for cancer therapy. with the aim of deregulating tumor pathways, and mimicking e4orf4 anti-cancer signal, we have previously proposed the dpt technology concept, based on design of specific pp1/pp2a interacting penetrating peptides.201323500018
impact of e1 and cre on adenovirus vector amplification: developing mdck cav-2-e1 and e1-cre transcomplementing cell lines.adenovirus vectors have been extensively studied through the manipulation of viral genome. however, little attention is being paid to their producer cell-lines; cells are selected according to virus yields, neglecting the expression profile of transcomplementing gene products underlying cell performance. this work evaluates the impact of e1 (e1a and e1b) and cre recombinase levels in the production of e1-deleted and helper-dependent canine adenovirus type 2 (cav-2) vectors using mdck cells. e1a ...201323565229
combined antiapoptotic and antioxidant approach to acute neuroprotection for stroke in hypertensive rats.we hypothesized that targeting key points in the ischemic cascade with combined neuroglobin (ngb) overexpression and c-jun n-terminal kinase (jnk) inhibition (sp600125) would offer greater neuroprotection than single treatment after in vitro hypoxia/reoxygenation and in a randomized, blinded in vivo experimental stroke study using a clinically relevant rat strain. male spontaneously hypertensive stroke-prone rats underwent transient middle cerebral artery occlusion (tmcao) and were divided into ...201323632970
high prevalence of antibodies against canine adenovirus (cav) type 2 in domestic dog populations in south africa precludes the use of cav-based recombinant rabies vaccines.rabies in dogs can be controlled through mass vaccination. oral vaccination of domestic dogs would be useful in the developing world, where greater vaccination coverage is needed especially in inaccessible areas or places with large numbers of free-roaming dogs. from this perspective, recent research has focused on development of new recombinant vaccines that can be administered orally in a bait to be used as adjunct for parenteral vaccination. one such candidate, a recombinant canine adenovirus ...201323867013
canine adenovirus type 2 vector generation via i-sce1-mediated intracellular genome release.when canine adenovirus type 2 (cadv-2, or also commonly referred to as cav-2) vectors are injected into the brain parenchyma they preferentially transduce neurons, are capable of efficient axonal transport to afferent regions, and allow transgene expression for at last >1 yr. yet, translating these data into a user-friendly vector platform has been limited because cav-2 vector generation is challenging. generation of e1-deleted adenovirus vectors often requires transfection of linear dna fragmen ...201323936483
canine adenovirus downstream processing protocol.adenovirus vectors are efficient gene delivery tools. a major caveat with vectors derived from common human adenovirus serotypes is that most adults are likely to have been exposed to the wild-type virus and exhibit active immunity against the vectors. this preexisting immunity limits their clinical success. strategies to circumvent this problem include the use of nonhuman adenovirus vectors. vectors derived from canine adenovirus type 2 (cav-2) are among the best-studied representatives. cav-2 ...201424132487
the serological and virological investigation of canine adenovirus infection on the dogs.two types of canine adenovirus (cavs), canine adenovirus type 1 (cav-1), the virus which causes infectious canine hepatitis, and canine adenovirus type 2 (cav-2), which causes canine infectious laryngotracheitis, have been found in dogs. in this study, blood samples taken from 111 dogs, which were admitted to the internal medicine clinic of selcuk university, faculty of veterinary medicine, with clinical symptoms. seventy-seven dogs were sampled from isparta and burdur dog shelters by random sam ...201324223508
central nervous system delivery of helper-dependent canine adenovirus corrects neuropathology and behavior in mucopolysaccharidosis type vii mice.canine adenovirus type 2 vectors (cav-2) are promising tools to treat global central nervous system (cns) disorders because of their preferential transduction of neurons and efficient retrograde axonal transport. here we tested the potential of a helper-dependent cav-2 vector expressing β-glucuronidase (hd-rigie) in a mouse model of mucopolysaccharidosis type vii (mps vii), a lysosomal storage disease caused by deficiency in β-glucuronidase activity. mps vii leads to glycosaminoglycan accumulati ...201424299455
production and purification of non replicative canine adenovirus type 2 derived vectors.adenovirus (ad) derived vectors have been widely used for short or long-term gene transfer, both for gene therapy and vaccine applications. because of the frequent pre-existing immunity against the classically used human adenovirus type 5, canine adenovirus type 2 (cav2) has been proposed as an alternative vector for human gene transfer. the well-characterized biology of cav2, together with its ease of genetic manipulation, offer major advantages, notably for gene transfer into the central nervo ...201324326926
a prb-responsive, rgd-modified, and hyaluronidase-armed canine oncolytic adenovirus for application in veterinary oncology.human and canine cancer share similarities such as genetic and molecular aspects, biological complexity, tumor epidemiology, and targeted therapeutic treatment. lack of good animal models for human adenovirotherapy has spurred the use of canine adenovirus 2-based oncolytic viruses. we have constructed a canine oncolytic virus that mimics the characteristics of our previously published human adenovirus icovir17: expression of e1a controlled by e2f sites, deletion of the prb-binding site of e1a, i ...201424448161
corrective gusb transfer to the canine mucopolysaccharidosis vii cornea using a helper-dependent canine adenovirus vector.corneal transparency is maintained, in part, by specialized fibroblasts called keratocytes, which reside in the fibrous lamellae of the stroma. corneal clouding, a condition that impairs visual acuity, is associated with numerous diseases, including mucopolysaccharidosis (mps) type vii. mps vii is due to deficiency in β-glucuronidase (β-glu) enzymatic activity, which leads to accumulation of glycosaminoglycans (gags), and secondary accumulation of gangliosides. here, we tested the efficacy of ca ...201424607662
combined use of the canine adenovirus-2 and dreadd-technology to activate specific neural pathways in vivo.we here describe a technique to transiently activate specific neural pathways in vivo. it comprises the combined use of a cre-recombinase expressing canine adenovirus-2 (cav-2) and an adeno-associated virus (aav-hsyn-dio-hm3d(gq)-mcherry) that contains the floxed inverted sequence of the designer receptor exclusively activated by designer drugs (dreadd) hm3d(gq)-mcherry. cav-2 retrogradely infects projection neurons, which allowed us to specifically express hm3d(gq)-mcherry in neurons that proje ...201424736748
detection of respiratory viruses and bordetella bronchiseptica in dogs with acute respiratory tract infections.canine infectious respiratory disease (cird) is an acute, highly contagious disease complex caused by a variety of infectious agents. at present, the role of viral and bacterial components as primary or secondary pathogens in cird is not fully understood. the aim of this study was to investigate the prevalence of canine parainfluenza virus (cpiv), canine adenovirus type 2 (cav-2), canine influenza virus (civ), canine respiratory coronavirus (crcov), canine herpes virus-1 (chv-1), canine distempe ...201424980809
rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction.canine distemper virus (cdv) has been associated with outbreaks of canine infectious respiratory disease in shelters and boarding kennel environments. pockittm nucleic acid analyzer is a field-deployable device capable of generating automatically interpreted insulated isothermal polymerase chain reaction (iipcr) results from extracted nucleic acid within one hour. in this study, reverse transcription iipcr (rt-iipcr) was developed to facilitate point-of-need diagnosis of cdv infection.201425200113
fatal canid herpesvirus 1 respiratory infections in 4 clinically healthy adult dogs.four healthy adult dogs (golden retrievers aged 6 years and 9 years, dalmatian aged 13 years, and mastiff aged 5 years) developed clinical signs of acute respiratory disease and died within 2 to 7 days of onset of clinical signs. the lungs of the 3 dogs submitted for necropsy were diffusely and severely reddened due to hyperemia and hemorrhage. microscopic lesions in all dogs were suggestive of acute viral or toxic respiratory damage and varied from acute severe fibrinonecrotic or hemorrhagic br ...201525358536
expression of vp7, a bluetongue virus group specific antigen by viral vectors: analysis of the induced immune responses and evaluation of protective potential in sheep.bluetongue virus (btv) is an economically important orbivirus transmitted by biting midges to domestic and wild ruminants. the need for new vaccines has been highlighted by the occurrence of repeated outbreaks caused by different btv serotypes since 1998. the major group-reactive antigen of btv, vp7, is conserved in the 26 serotypes described so far, and its role in the induction of protective immunity has been proposed. viral-based vectors as antigen delivery systems display considerable promis ...201425364822
serologic evidence for selected infectious diseases in marsican brown bears (ursus arctos marsicanus) in italy (2004-09).we tested 30 serum samples collected during 2004-09 from 22 free-ranging marsican brown bears (ursus arctos marsicanus) in the national park of abruzzo, lazio, and molise, italy, for antibodies against canine distemper virus (cdv), canine adenovirus type 2 (cav-2), canine parvovirus type 2 (cpv-2), brucella spp., and eight leptospira interrogans sensu lato serovars. antibody to cdv was detected in 11 samples (37%); only two bears (10%) had detectable cav-2 and brucella spp. antibodies; three bea ...201525375945
a simplified system for generating recombinant e3-deleted canine adenovirus-2.canine adenovirus type 2 (cav-2) has been used extensively as a vector for studying gene therapy and vaccine applications. we describe a simple strategy for generating a replication-competent recombinant cav-2 using a backbone vector and a shuttle vector. the backbone plasmid containing the full-length cav-2 genome was constructed by homologous recombination in escherichia coli strain bj5183. the shuttle plasmid, which has a deletion of 1478 bp in the nonessential e3 viral genome region, was gen ...201525450764
antiviral activity of a bacillus sp. p34 peptide against pathogenic viruses of domestic animals.p34 is an antimicrobial peptide produced by a bacillus sp. strain isolated from the intestinal contents of a fish in the brazilian amazon basin with reported antibacterial activity. the aim of this work was to evaluate the peptide p34 for its in vitro antiviral properties against canine adenovirus type 2 (cav-2), canine coronavirus (ccov), canine distemper virus (cdv), canine parvovirus type 2 (cpv-2), equine arteritis virus (eav), equine influenza virus (eiv), feline calicivirus (fcv) and felin ...201425477947
canine helper-dependent vectors production: implications of cre activity and co-infection on adenovirus propagation.the importance of cre recombinase to minimize helper vector (hv) contamination during helper-dependent adenovirus vectors (hdvs) production is well documented. however, cre recombinase, by inducing dna double-strand breaks (dsbs), can cause a reduced proliferation and genotoxic effects in cultured cells. in this work, cre-expressing cell stability, co-infection and their relation to adenovirus amplification/hv contamination were evaluated to develop a production protocol for hd canine adenovirus ...201525774853
protective efficacy of recombinant canine adenovirus type-2 expressing tgrop18 (cav-2-rop18) against acute and chronic toxoplasma gondii infection in mice.the use of recombinant viral vectors expressing t. gondii antigens is a safe and efficient approach to induce immune responses against the parasite, as well as a valuable tool for vaccine development. we have previously prolonged the survival time of mice challenged with the rh strain of t. gondii by immunizing the mice with a eukaryotic vector expressing the protein rop18 of t. gondii. we are now looking for ways to improve this vaccination strategy and enhance protection.201525886737
5-ht1b autoreceptors differentially modulate the expression of conditioned fear in a circuit-specific manner.located in the nerve terminals of serotonergic neurons, 5-ht1b autoreceptors are poised to modulate synaptic 5-ht levels with precise temporal and spatial control, and play an important role in various emotional behaviors. this study characterized two novel, complementary viral vector strategies to investigate the contribution of 5-ht1b autoreceptors to fear expression, displayed as freezing, during contextual fear conditioning. increased expression of 5-ht1b autoreceptors throughout the brain s ...201525907441
production of canine adenovirus type 2 in serum-free suspension cultures of mdck cells.the potential of adherent madin darby canine kidney (mdck) cells for the production of influenza viruses and canine adenovirus type 2 (cav-2) for vaccines or gene therapy approaches has been shown. recently, a new mdck cell line (mdck.sus2) that was able to grow in suspension in a fully defined system was established. in this work, we investigated whether the new mdck.sus2 suspension cell line is suitable for the amplification of cav-2 under serum-free culture conditions. cell growth performance ...201525994255
development of a sybr green real-time pcr assay with melting curve analysis for simultaneous detection and differentiation of canine adenovirus type 1 and type 2.canine adenovirus type 1 (cadv-1) and canine adenovirus type 2 (cadv-2) cause infectious canine hepatitis (ich) and infectious tracheobronchitis (itb) in dogs, respectively. cases of ich have been documented in recent years and recent surveys have demonstrated a wide percentage of asymptomatic cadv-1 infection in the canine population. since both cadv types are detectable in the same biological matrices, and viral coinfection with cadv-1 and cadv-2 are reported with high frequency, it is urgent ...201526028428
the intracellular domain of the coxsackievirus and adenovirus receptor differentially influences adenovirus entry.the coxsackievirus and adenovirus receptor (car) is a cell adhesion molecule used as a docking molecule by some adenoviruses (advs) and group b coxsackieviruses. we previously proposed that the preferential transduction of neurons by canine adenovirus type 2 (cav-2) is due to car-mediated internalization. our proposed pathway of cav-2 entry is in contrast to that of human adv type 5 (hadv-c5) in nonneuronal cells, where internalization is mediated by auxiliary receptors such as integrins. we the ...201526136571
evaluation of helper-dependent canine adenovirus vectors in a 3d human cns model.gene therapy is a promising approach with enormous potential for treatment of neurodegenerative disorders. viral vectors derived from canine adenovirus type 2 (cav-2) present attractive features for gene delivery strategies in the human brain, by preferentially transducing neurons, are capable of efficient axonal transport to afferent brain structures, have a 30-kb cloning capacity and have low innate and induced immunogenicity in preclinical tests. for clinical translation, in-depth preclinical ...201626181626
prevalence of canine infectious respiratory pathogens in asymptomatic dogs presented at us animal shelters.to determine the prevalence of nine canine infectious respiratory disease (cird) pathogens in asymptomatic dogs presented at animal shelters across the united states.201526199194
transcriptional response of human neurospheres to helper-dependent cav-2 vectors involves the modulation of dna damage response, microtubule and centromere gene groups.brain gene transfer using viral vectors will likely become a therapeutic option for several disorders. helper-dependent (hd) canine adenovirus type 2 vectors (cav-2) are well suited for this goal. these vectors are poorly immunogenic, efficiently transduce neurons, are retrogradely transported to afferent structures in the brain and lead to long-term transgene expression. cav-2 vectors are being exploited to unravel behavior, cognition, neural networks, axonal transport and therapy for orphan di ...201526207738
cav-2--why a canine virus is a neurobiologist's best friend.canine adenovirus type 2 (cav-2) vectors are powerful tools for fundamental and applied neurobiology due to their negligible immunogenicity, preferential transduction of neurons, widespread distribution via axonal transport, and duration of expression in the mammalian brain. cav-2 vectors are internalized in neurons by the selective use of coxsackievirus and adenovirus receptor (car), which is located at the presynapse in neurons. neuronal internalization and axonal transport is mediated by car, ...201526298516
adenovirus 2, bordetella bronchiseptica, and parainfluenza molecular diagnostic assay results in puppies after vaccination with modified live vaccines.canine adenovirus 2, parainfluenza, and bordetella bronchiseptica cause respiratory disease in dogs, and each has a modified live intranasal vaccine available. molecular diagnostic assays to amplify specific nucleic acids are available for each of these agents. if positive molecular diagnostic assay results are common after vaccination, the positive predictive value of the diagnostic assays for disease would be decreased.201526692461
age-related presence of selected viral and bacterial pathogens in paraffin-embedded lung samples of dogs with pneumonia.the aim of this retrospective study was to detect selected pathogens in pneumonic lung tissue of dogs of different age groups by immunohistochemistry (ihc), in situ hybridisation (ish) or polymerase chain reaction (pcr) in order to get information about their involvement in pneumonia formation. in archived formalin-fixed and paraffin wax-embedded lung samples from 68 cases with the clinical and histologic diagnosis of pneumonia the histological pattern of pneumonia was re-evaluated and the sampl ...201626919147
a duplex real-time pcr assay based on taqman technology for simultaneous detection and differentiation of canine adenovirus types 1 and 2.canine adenoviruses are a major cause of disease in dogs, coyotes, red foxes and wolves, as well as in other carnivores and marine mammals. canine adenovirus type 1 (cadv-1) and canine adenovirus type 2 (cadv-2) cause infectious canine hepatitis (ich) and infectious tracheobronchitis (itb), respectively. in this study, a duplex real-time pcr assay for simultaneous detection and characterisation of cadv-1 and cadv-2 was developed by using a single primer pair and virus-specific probes. the assay ...201627040113
detection of respiratory viruses in shelter dogs maintained under varying environmental conditions.three dog shelters in rio grande do sul were investigated for associations between the occurrence of respiratory viruses and shelter environmental conditions. nasal secretions randomly collected during the cold season were tested via pcr, and this data collection was followed by nucleotide sequencing of the amplicons. in shelter #1 (poor sanitary and nutritional conditions, high animal density and constant contact between dogs), 78% (58/74) of the nasal samples were positive, 35% (26/74) of whic ...201727522932
development and application of multiplex pcr assays for detection of virus-induced respiratory disease complex in dogs.canine infectious respiratory disease complex (cirdc) viruses have been detected in dogs with respiratory illness. canine influenza virus (civ), canine parainfluenza virus (cpiv), canine distemper virus (cdv), canine respiratory coronavirus (crcov), canine adenovirus type 2 (cadv-2) and canine herpesvirus 1 (cahv-1), are all associated with the cirdc. to allow diagnosis, two conventional multiplex polymerase chain reactions (pcr) were developed to simultaneously identify four rna and two dna vir ...201627628592
recombinant canine adenovirus type-2 expressing tgrop16 provides partial protection against acute toxoplasma gondii infection in mice.we previously demonstrated that the survival time of balb/c mice challenged with toxoplasma gondii rh strain was prolonged by immunising the mice with a eukaryotic vector expressing the protein rop16 of t. gondii. building upon previous findings, we are exploring improved vaccination strategies to enhance protection. in this work, a novel recombinant canine adenovirus type 2 expressing rop16 (cav-2-rop16) of t. gondii was constructed and identified to express rop16 in madin-darby canine kidney c ...201627742446
serological and molecular epidemiology of canine adenovirus type 1 in red foxes (vulpes vulpes) in the united kingdom.canine adenovirus type 1 (cav-1) causes infectious canine hepatitis (ich), a frequently fatal disease which primarily affects canids. in this study, serology (elisa) and molecular techniques (pcr/qpcr) were utilised to investigate the exposure of free-ranging red foxes (vulpes vulpes) to cav-1 in the united kingdom (uk) and to examine their role as a wildlife reservoir of infection for susceptible species. the role of canine adenovirus type 2 (cav-2), primarily a respiratory pathogen, was also e ...201627796367
excitatory hindbrain-forebrain communication is required for cisplatin-induced anorexia and weight loss.cisplatin chemotherapy is commonly used to treat cancer despite severe energy balance side effects. in rats, cisplatin activates nucleus tractus solitarius (nts) projections to the lateral parabrachial nucleus (lpbn) and calcitonin-gene related peptide (cgrp) projections from the lpbn to the central nucleus of the amygdala (cea). we demonstrated previously that cea glutamate receptor signaling mediates cisplatin-induced anorexia and body weight loss. here, we used neuroanatomical tracing, immuno ...201728077715
detection of canine adenovirus 1 in red foxes ( vulpes vulpes) and raccoons ( procyon lotor) in germany with a taqman real-time pcr assay.we developed a real-time (rt)pcr assay based on taqman probe technology for the specific detection of canine adenovirus 1 (cadv-1). the assay is able to detect three 50% tissue culture infectious dose/ml in cadv-1-containing cell culture supernatant. viral genomes were not amplified of canine adenovirus 2 or of several bovine, porcine, and avian adenoviruses. in silico analysis provided no indication of amplification of other heterologous genomes. the sensitivity of the real-time assay exceeded ...201728581363
surveillance for antibodies against six canine viruses in wild raccoons (procyon lotor) in japan.raccoons (procyon lotor) are found worldwide. they are frequently seen in crowded inner cities as well as in forests or wooded areas, often living in proximity to humans and their pets. we examined sera from 100 wild raccoons in japan for antibodies to six canine viruses with veterinary significance to assess their potential as reservoirs. we also aimed to understand the distribution of potentially infected wildlife. we found that 7% of samples were seropositive for canine distemper virus (cdv), ...201728715293
european surveillance of emerging pathogens associated with canine infectious respiratory disease.canine infectious respiratory disease (cird) is a major cause of morbidity in dogs worldwide, and is associated with a number of new and emerging pathogens. in a large multi-centre european study the prevalences of four key emerging cird pathogens; canine respiratory coronavirus (crcov), canine pneumovirus (cnpnv), influenza a, and mycoplasma cynos (m. cynos); were estimated, and risk factors for exposure, infection and clinical disease were investigated. cird affected 66% (381/572) of the dogs ...201729173585
evaluation of protective efficacy of three novel h3n2 canine influenza vaccines.canine influenza virus (civ) has the potential risk to spread in different areas and dog types. thus, there is a growing need to develop an effective vaccine to control civ disease. here, we developed three vaccine candidates: 1) a recombinant pvax1 vector expressing h3n2 civ hemagglutinin (pvax1-ha); 2) a live attenuated canine adenovirus type 2 expressing h3n2 civ hemagglutinin (rcav2-ha); and 3) an inactivated h3n2 civ (a/canine/guangdong/01/2006 (h3n2)). mice received an initial intramuscula ...201729228675
recombinant canine adenovirus type 2 expressing rabbit hemorrhagic disease virus vp60 protein provided protection against rhd in rabbits.rabbit hemorrhagic disease virus (rhdv) is responsible for rabbit hemorrhagic disease (rhd), which is an acute, lethal and highly contagious disease in both wild and domestic rabbits. although current vaccines are highly effective for controlling rhd, they are derived from infected rabbit livers and their use is thus associated with safety and animal-welfare concerns. in this study, we generated a recombinant lentogenic canine adenovirus type 2 (cav2) vector expressing the rhdv vp60 gene, named ...201829291998
genetic dissection of the neuro-glio-vascular machinery in the adult brain.the adult brain actively controls its metabolic homeostasis via the circulatory system at the blood brain barrier interface. the mechanisms underlying the functional coupling from neuron to vessel remain poorly understood. here, we established a novel method to genetically isolate the individual components of this coupling machinery using a combination of viral vectors. we first discovered a surprising non-uniformity of the glio-vascular structure in different brain regions. we carried out a vir ...201829335006
a canine adenovirus type 2 vaccine vector confers protection against foot-and-mouth disease in guinea pigs.vaccination is a key element in the control of foot-and-mouth disease (fmd). the majority of the antigenic sites that induce protective immune responses are localized on the fmd virus (fmdv) capsid that is formed by four virus-encoded structural proteins, vp1 to vp4. in the present study, recombinant canine adenovirus type 2 (cav2)-based fmd vaccines, cav-p1/3c r° and cav-vp1 r°, respectively expressing the structural p1 precursor protein along with the non-structural 3c protein or expressing th ...201829544690
sequential circulation of canine adenoviruses 1 and 2 in captive wild carnivores, france.scarce data are currently available about the ecology of canine adenoviruses (cadvs) in wild carnivores. in this paper, the consecutive circulation of cadv-1 and cadv-2 in wild carnivores maintained in a french zoological park is reported. a fatal cadv-1 infection was observed in a eurasian wolf (canis lupus lupus), which displayed gross lesions, histopathological changes and immunohistochemical findings suggestive of cadv-1 infection. the virus was isolated on cell cultures and its genome was d ...201829981710
exogenous lrrk2g2019s induces parkinsonian-like pathology in a nonhuman primate.parkinson's disease (pd) is the second most prevalent neurodegenerative disease among the elderly. to understand its pathogenesis and to test therapies, animal models that faithfully reproduce key pathological pd hallmarks are needed. as a prelude to developing a model of pd, we tested the tropism, efficacy, biodistribution, and transcriptional effect of canine adenovirus type 2 (cav-2) vectors in the brain of microcebus murinus, a nonhuman primate that naturally develops neurodegenerative lesio ...201830046008
identification of pantropic canine coronavirus in a wolf ( canis lupus italicus) in italy.we report a case in an italian wolf ( canis lupus italicus) of pantropic canine coronavirus infection, which has previously been detected only in dogs. the wolf was coinfected by canine parvovirus type 2b and canine adenovirus type 2, which highlighted the crucial role of epidemiologic surveys in european wild carnivores.201930376395
serum biochemistry values and select serologic screening of brown hyenas ( parahyaena brunnea) from the namib desert, namibia.blood from 30 free-ranging brown hyenas ( parahyaena brunnea) was collected for biochemical analysis and select serologic screening in namibia from 1997 to 2010. age was found to have an influence on several biochemical parameters that may be related to growth, a developing immune system, and differences in diet. seasonal differences in diet of coastal brown hyenas also had an overall significant effect on lipemia values, and differences in stress due to varying capture methods could be associat ...201830592906
transduction of craniofacial motoneurons following intramuscular injections of canine adenovirus type-2 (cav-2) in rhesus macaques.reliable viral vector-mediated transgene expression in primate motoneurons would improve our ability to anatomically and physiologically interrogate motor systems. we therefore investigated the efficacy of replication defective, early region 1-deleted canine adenovirus type-2 (cav-2) vectors for mediating transgene expression of fluorescent proteins into brainstem motoneurons following craniofacial intramuscular injections in four rhesus monkeys (macaca mulatta). vector injections were placed in ...201931619971
immunogenicity of a new, inactivated canine adenovirus type 2 vaccine for dogs.we constructed a new canine adenovirus type 2 (cav-2) vaccine candidate using the recently isolated korean cav-2 strain; we termed the vaccine apqa1701-40p and evaluated its safety and immunogenicity in dogs.202032095439
detection of selected pathogens in apennine wolf (canis lupus italicus) by a non-invasive gps-based telemetry sampling of two packs from majella national park, italy.in this study, a multi-pathogens survey was conducted to verify the sanitary status of two italian wolf packs of majella national park. twenty fecal samples (10/pack) were collected using a sampling protocol, based on the combining data from radio-collared wolves with geographic information system (gis) analysis, allowing to mark off the home range of packs and to recover group-specific and high-quality specimens. virological screening against the most prevalent canine viruses (protoparvovirus, ...201932214950
cav-2-mediated gfp and lrrk2g2019s expression in the macaca fascicularis brain.parkinson's disease is characterized by motor and nonmotor symptoms that gradually appear as a consequence of the selective loss of dopaminergic neurons in the substantia nigra pars compacta. currently, no treatment can slow parkinson's disease progression. inasmuch, there is a need to develop animal models that can be used to understand the pathophysiological mechanisms underlying dopaminergic neuron death. the initial goal of this study was to determine if canine adenovirus type 2 (cav-2) vect ...202032269512
molecular evidence for vaccine-induced canine distemper virus and canine adenovirus 2 coinfection in a fennec fox.a 61-d-old fennec fox (vulpes zerda), 11 d after receiving a multivalent, modified-live virus vaccine containing canine distemper virus (cdv), canine adenovirus 2 (cadv-2), parainfluenza virus, parvovirus, and canine coronavirus, developed oculonasal discharge, and subsequently convulsions, and hemoptysis, and died. microscopic changes in the cerebrum were evident, including neuronal degeneration and necrosis; intracytoplasmic eosinophilic inclusion bodies were observed in astrocytes. cdv was de ...202032560597
Refresh
firstprev
12
nextlast
Displaying items 101 - 162 of 162