Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| equilibrium and kinetic analysis of autographa californica nuclear polyhedrosis virus attachment to different insect cell lines. | the kinetic and equilibrium attachment of autographa californica nuclear polyhedrosis virus (acmnpv) to seven insect cell lines was evaluated. kinetic experiments revealed differences of up to 10-fold in the infection rates among cell lines. equilibrium binding also varied between cell lines and was saturable. the tn 5b1-4 and tn f cell lines had the highest virus binding affinities and infection rates and exhibited diffusion-limited attachment. the rate of infection appears to be limited by the ... | 1992 | 1469356 |
| protein synthesis in pupae of the silkworm bombyx mori after infection with nuclear polyhedrosis virus: resistance to viral infection acquired during pupal period. | protein synthesis has been studied in pupae of the silkworm bombyx mori (bm) infected with nuclear polyhedrosis virus (bmnpv) at various stages of the pupal period. nascent proteins were labelled by injection of [35s]methionine into pupae and then analysed by sds-page. temporal regulation of synthesis of infected cell-specific proteins (icsps) in pupae was demonstrated by electrophoretic analysis of the proteins labelled at different times post-infection (p.i.). the rate of icsp synthesis reache ... | 1992 | 1469357 |
| induction of a novel protein kinase in pupae of the silkworm bombyx mori after infection with nuclear polyhedrosis virus. | protein kinases induced by bombyx mori nuclear polyhedrosis virus in pupae of the silkworm b. mori were examined by activity gel analysis using phosvitin as a protein substrate. the method involved page of the soluble fraction from pupae under native conditions and in the presence of sds, followed by in situ renaturation of proteins and recovery of protein kinase activity in the intact gel. a novel protein kinase able to phosphorylate phosvitin was detected in the infected pupae from 2 days post ... | 1992 | 1469362 |
| transient expression assay in a baculovirus system using firefly luciferase gene as a reporter. | transient gene expression assays were developed to assess the function of the regulatory sequences of baculoviruses bombyx mori nuclear polyhedrosis virus (bmnpv) and autographa californica nuclear polyhedrosis virus (acnpv) in insect cells of bombyx mori and spodoptera frugiperda, respectively. dna sequences encoding luciferase (luc) of the firefly photinus pyralis was successfully employed in the expression assay as a reporter gene. recombinant plasmids were constructed containing the luc gene ... | 1992 | 1475909 |
| characterization of baculovirus-expressed hemagglutinin and fusion glycoproteins of the attenuated measles virus strain aik-c. | with measles virus cdna of the avirulent vaccine strain aik-c, two cdnas of h or f genes were amplified by the polymerase chain reaction. the amplified cdnas were inserted respectively to the baculovirus transfer vector pacym1 derived from the nuclear polyhedrosis virus of autographa californica (acnpv). after co-transfection of the transfer vectors with acnpv dna to spodoptera frugiperda cells, recombinant baculoviruses were screened by plaque assay, and the viruses containing h-cdna or f-cdna ... | 1992 | 1476005 |
| sequence and in vitro translational analysis of a 1629-nucleotide orf in autographa californica nuclear polyhedrosis virus strain e2. | the complete nucleotide (nt) sequence of an open reading frame (orf) (map unit 5.1 to 3.8) from autographa californica nuclear polyhedrosis virus strain e2 (acmnpv-e2) has been determined. this 1629-nt orf has a coding potential for a 61-kda pro-rich protein. however, in vitro translation of the 1629-nt orf and sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (sds-page) revealed a 78-kda protein product. the discrepancy between the m(r) predicted by the nt sequence and that obtained fro ... | 1992 | 1487149 |
| site-specific mutagenesis of the 35-kilodalton protein gene encoded by autographa californica nuclear polyhedrosis virus: cell line-specific effects on virus replication. | the gene encoding the 35-kda protein (35k gene) located within the ecori-s genome fragment of autographa californica nuclear polyhedrosis virus (acmnpv) is transcribed early in infection. to examine its function(s) with respect to virus multiplication, we introduced specific mutations of this early gene into the acmnpv genome. in spodoptera frugiperda (sf21) culture, deletion of the 35k gene reduced yields of extracellular, budded virus from 200- to 15,000-fold, depending on input multiplicity. ... | 1992 | 1501287 |
| a synthetic early promoter from a baculovirus: roles of the tata box and conserved start site cagt sequence in basal levels of transcription. | many baculovirus early genes and insect genes transcribed by rna polymerase ii have a conserved transcription start site sequence (cagt) located downstream of a consensus tata box. to examine the functions and interactions of these two motifs in initiating accurately positioned basal transcription, a 43-nt synthetic promoter was synthesized from the tata box and start site sequences of the gp64 early promoter from the orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (opmnpv). the synt ... | 1992 | 1519359 |
| a cellular promoter-based expression cassette for generating recombinant baculoviruses directing rapid expression of passenger genes in infected insects. | we have developed an expression cassette which allows the generation of recombinant baculoviruses that can express passenger genes under the control of a constitutive cellular promoter derived from the cytoplasmic actin gene of the silkmoth bombyx mori. silkmoth tissue culture cells which were infected with a recombinant b. mori nuclear polyhedrosis virus (bmnpv) containing the gene-encoding chloramphenicol acetyltransferase (cat) under the control of this expression cassette expressed significa ... | 1992 | 1519361 |
| replication of autographa californica baculovirus (acmnpv) in a coleopteran cell line. | a coleopteran cell line (age) derived from the cotton boll weevil anthonomus grandis supported replication of autographa californica multiple nuclear polyhedrosis virus (acmnpv). the titer of extracellular virus (ecv) and the number of occlusion bodies (ob) produced in age cells were approximately equal to those produced by a trichoplusia ni cell line (tn-cl1), and the ob produced by both cell lines were equally infectious for t. ni larvae. the identity of the age cell line was established by ch ... | 1992 | 1522049 |
| the autographa californica baculovirus genome: evidence for multiple replication origins. | the autographa californica multinucleocapsid nuclear polyhedrosis virus (acmnpv), which is used for the overexpression of eukaryotic genes and is being engineered for possible use as a viral insecticide, has a circular, supercoiled genome of approximately 128 kilobases. despite its widespread use, little is known about the mechanism by which acmnpv replicates. evidence is presented in this report that acmnpv origins of dna replication are repeated sequences each containing several closely relate ... | 1992 | 1529337 |
| nucleotide sequence and transcriptional analysis of the p80 gene of autographa californica nuclear polyhedrosis virus: a homologue of the orgyia pseudotsugata nuclear polyhedrosis virus capsid-associated gene. | the 67.2- to 68.5-m.u. region of autographa californica nuclear polyhedrosis virus was sequenced. a large open reading frame (orf) was identified in the clockwise direction on the circular genome map which could potentially encode an 80-kda polypeptide. analysis of the predicted amino acid sequence of this orf indicated that it was a homologue of the p87 capsid-associated gene of orgyia pseudotsugata mnpv with an overall amino acid similarity of 34.3%. a late transcript of 2.1 kb was mapped to t ... | 1992 | 1529529 |
| in vivo transcriptional analysis of three baculovirus genes: evidence of homology between viral and host transcripts. | transcripts for the gp64 and polyhedrin genes as well as a 1629 open reading frame (1629 orf) of the autographa californica nuclear polyhedrosis virus were examined in the midgut tissues and hemocytes of uninfected and infected host trichoplusia ni larvae and sf21 cells. polyhedrin-specific transcripts of 1.2, 3.4, and 4.9 kb were expressed in both infected larval tissues and infected sf21 cells. the highest level of expression for polyhedrin-specific transcripts was observed in hemocytes, where ... | 1992 | 1529534 |
| hepatitis delta antigen expressed by recombinant baculoviruses: comparison of biochemical properties and post-translational modifications between the large and small forms. | hepatitis delta virus (hdv) encodes only one protein, the hepatitis delta antigen (hdag). two forms of hdag, a large (27 kda) and a small (24 kda) one, participate in the various steps of hdv replication. to further understand the properties of hdag, we have constructed recombinant baculoviruses and expressed both forms of the hdag in insect cells. the gene encoding hdag was placed under the control of the polyhedrin promoter of autographa californica nuclear polyhedrosis virus (acnpv) by homolo ... | 1992 | 1529540 |
| expression of the nucleocapsid protein of dugbe virus and antigenic cross-reactions with other nairoviruses. | the small (s) rna segment of dugbe (dug) virus (nairovirus, bunyaviridae) encodes a single protein, the nucleocapsid (n) protein, of m(r) 49.4 kda. cdna derived from the complete coding region for the n protein was cloned into autographa californica nuclear polyhedrosis virus (acnpv) under control of the polyhedrin promoter and used to infect spodoptera frugiperda insect cells. western blotting analysis using monoclonal antibodies demonstrated the production of dug n protein in the infected cell ... | 1992 | 1529645 |
| nucleotide sequence of a transactivating bombyx mori nuclear polyhedrosis virus immediate early gene. | the open reading frame (orf) of 1572 bp contained in the 3.8 kb clai fragment of bmnpv encodes a viral regulatory protein which transactivates the delayed early acmnpv 39k gene. transactivation is induced in uninfected cells following transfection with a plasmid containing only the clai fragment. hitherto immediate early gene promoter activity of the included 631 bp leader sequence is evident since no other viral elements are needed for the transcription of the regulatory gene. | 1992 | 1536885 |
| biophysical and biochemical properties of baculovirus-expressed camv p1 protein. | cauliflower mosaic virus (camv) gene i encodes a protein (p1) that has been implicated in the control of virus movement in infected plants. to assist in the characterization of the mechanism of action of p1, gene i has been expressed efficiently in spodoptera frugiperda (sf) cells using recombinant baculovirus. control of the expression of camv gene i by the polyhedrin late promoter in the baculovirus autographa californica nuclear polyhedrosis virus (acnpv) resulted in very high levels of p1 ac ... | 1992 | 1549910 |
| expression of the autographa californica nuclear polyhedrosis virus p10 gene: effect of polyhedrin gene expression. | two major late proteins, polyhedrin and p10, are synthesized in large quantities in baculovirus infected insect cells. this and the fact that both proteins are dispensable for virus replication, form the basis for the use of these viruses as vector for foreign gene expression. to address the question whether the autographa californica nuclear polyhedrosis virus p10 promotor-driven expression is influenced by the concurrent expression of the polyhedrin gene, several recombinants were constructed ... | 1992 | 1550489 |
| viral and host cellular transcription in autographa californica nuclear polyhedrosis virus-infected gypsy moth cell lines. | infection of two gypsy moth cell lines (iplb-ld652y and iplb-ldfb) by the autographa californica multiple-enveloped nuclear polyhedrosis virus (acmnpv) is characterized by extremely attenuated viral protein synthesis followed by a total arrest of all viral and cellular protein production. in this study, acmnpv- and host cell-specific transcription were examined. overall levels of viral rnas in infected gypsy moth cells were, at most measured times, comparable to rna levels from an infected cell ... | 1992 | 1560533 |
| expression and extracellular release of human immunodeficiency virus type 1 gag precursors by recombinant baculovirus-infected cells. | the level of synthesis and extracellular release of human immunodeficiency virus type 1 gag by insect cells was analyzed, using eight different recombinants of autographa californica nuclear polyhedrosis virus harboring various constructs of the gag gene, cloned under the polyhedrin promoter. the results obtained suggested that gag expression was mainly regulated at the transcriptional level and was not significantly influenced by posttranslational events, e.g., gag self-assembly, nuclear transp ... | 1992 | 1560545 |
| a structural polypeptide of the baculovirus autographa californica nuclear polyhedrosis virus contains o-linked n-acetylglucosamine. | a structural glycopeptide, gp41, derived from the occluded virus of the baculovirus autographa californica nuclear polyhedrosis virus was characterized. the peptide specifically bound wheat germ agglutinin but was not recognized by a panel of seven other lectins. reactivity with wheat germ agglutinin was eliminated by treatment of gp41 with beta-n-acetylglucosaminidase, indicating that n-acetylglucosamine (glcnac) was present as terminal residues. gp41 was efficiently galactosylated by galactosy ... | 1992 | 1583718 |
| differential factor binding at the promoter of early baculovirus gene pe38 during viral infection: gata motif is recognized by an insect protein. | regulatory elements interacting with dna-binding proteins have been investigated in the promoter sequence of the early pe38 gene in the autographa californica nuclear polyhedrosis virus (acnpv). a gata motif located 50 nucleotides upstream of the pe38 transcriptional start site is recognized differentially in the course of infection. as demonstrated by footprint and gel mobility shift assays, the gata sequences ttatct are protected by nuclear extracts from uninfected spodoptera frugiperda cells ... | 1992 | 1583720 |
| regulation of delayed-early gene transcription by dual tata boxes. | the 39k autographa californica nuclear polyhedrosis virus (acmnpv) gene is highly expressed throughout the virus life cycle and is controlled by tandem promoters that exhibit features of early and late baculovirus promoters. late transcripts initiate at a conserved taag motif, while early transcripts are heterogeneous and initiate near a conserved cagt motif. to define the nucleotide sequences that regulate early transcription of the 39k gene, a series of mutations was generated by substitution ... | 1992 | 1583729 |
| purification and characterization of human salivary-gland prokallikrein from recombinant baculovirus-infected insect cells. | a full-length cdna encoding human salivary-gland preprokallikrein was inserted into the baculovirus autographa californica nuclear polyhedrosis virus downstream of the polyhedrin promoter. the gene was expressed in transfected spodoptera frugiperda cells and the recombinant product secreted into the culture medium. by alternating anion-exchange chromatography and gel-filtration steps, twice repeated, prokallikrein was purified to homogeneity, which was confirmed by amino acid analysis and n-term ... | 1992 | 1587272 |
| the anticarsia gemmatalis nuclear polyhedrosis virus polyhedrin gene region: sequence analysis, gene product and structural comparisons. | the genomic region of the anticarsia gemmatalis multiple nucleocapsid nuclear polyhedrosis virus (agmnpv) strain 2d encoding the polyhedrin gene was cloned and mapped, and a 2085 bp sphi-psti fragment containing the gene was sequenced. the polyhedrin polypeptide of the parental isolate agmnpv was manually sequenced, and the amino acid sequence obtained agreed with that deduced from the dna coding region sequence. agmnpv and orgyia pseudotsugata mnpv (opmnpv) are similar in terms of promoter stru ... | 1992 | 1588315 |
| localization of juvenile hormone esterase during development in normal and in recombinant baculovirus-infected larvae of the moth trichoplusia ni. | the pathogenesis and cellular localization of juvenile hormone esterase (jhe) was examined in larvae of the moth trichoplusia ni, infected with a recombinant baculovirus (autographa californica nuclear polyhedrosis virus: acnpv) engineered to produce high levels of jhe (jhe virus). the course of jhe localization in the recombinant virus infected larvae was compared with that of both wild type acnpv infected, and uninfected larvae, using immunogold electron microscopy. in the jhe virus infected i ... | 1992 | 1589874 |
| a simple method for the extraction of baculovirus dna. | we have developed a method using lysozyme for dna extraction from baculoviruses using as a model lymantria dispar nuclear polyhedrosis virus (ldnpv) obtained from infected larvae. this method proved to be quick, inexpensive and the extracted dna was successfully used in molecular hybridization experiments. | 1992 | 1597509 |
| secretion of a functional soluble form of neutral endopeptidase-24.11 from a baculovirus-infected insect cell line. | neutral endopeptidase (nep; ec 3.4.24.11) is an integral membrane protein found at the plasma membrane of many cell types. a secreted form of nep (sec-nep) was recently obtained by transfection of cos-1 cells with a recombinant expression vector consisting of the cdna encoding the signal peptide of pro-opiomelanocortin fused in-frame to the cdna sequence of the complete ectodomain of rabbit nep [lemay, waksman, roques, crine & boileau (1989) j. biol. chem. 264, 15620-15623]. in order to produce ... | 1992 | 1599410 |
| generation and analysis of defective genomes of autographa californica nuclear polyhedrosis virus. | we have generated defective genomes of autographa californica nuclear polyhedrosis virus (acnpv) by serial, undiluted passage in iplb-sf-21 cell culture in an attempt to identify potential cis-acting sequences important for acnpv dna replication. viral dna isolated from some of the 81 serial passages was analyzed by pulsed-field gel electrophoresis, restriction endonuclease analysis, and southern blot hybridization. acnpv-defective genomes appeared to be generated through a series of successivel ... | 1992 | 1602548 |
| the amino acid sequence determination of a granulin and polyhedrin from two baculoviruses infecting agrotis segetum. | the amino acid sequence of agrotis segetum granulosis virus (asgv) granulin and a. segetum nuclear polyhedrosis virus (asnpv) polyhedrin was determined by sequencing tryptic and chymotryptic peptides from reduced and carboxymethylated proteins and tryptic fragments of oxidized and maleylated granulin. the comparison of the established peptide structures with the primary structures of other occlusion body proteins from related baculoviruses was also used for the polypeptide chains' reconstruction ... | 1992 | 1604817 |
| analysis of polyhedra morphology mutants of autographa californica nuclear polyhedrosis virus: molecular and ultrastructural features. | two new mutants of autographa californica multiple nuclear polyhedrosis virus affected in the morphogenesis of their polyhedra, designated m276 and m934, were investigated. marker transfer experiments demonstrated that the observed phenotype was due exclusively to alterations in the polyhedrin gene. m276 contained a 229 base insertion near the carboxyl terminus coding region which resulted in synthesis of a truncated protein; m934 had a point mutation substituting phenylalanine for leucine at am ... | 1992 | 1607865 |
| dissimilar expression of autographa californica multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes. | the temporal expression of the autographa californica multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes in spodoptera frugiperda cells was studied using virus recombinants in which either gene was replaced by the juvenile hormone esterase (jhe) gene of heliothis virescens. the jhe served as a highly specific and sensitive reporter for gene expression. activation of the p10 gene followed a pattern different to that of polyhedrin. the p10 gene was activated a few hours earl ... | 1992 | 1607866 |
| nucleotide sequence of the polyhedron envelope protein gene region of the lymantria dispar nuclear polyhedrosis virus. | a 6.4 kb region from the lymantria dispar multicapsid nuclear polyhedrosis virus (ldmnpv) genome was sequenced and found to contain open reading frames (orfs) homologous to the polyhedron envelope (pe) protein coding sequence, and the c-terminal half of orf 1, which is a gene located upstream of the pe protein gene in other baculoviruses. the proteins predicted from the ldmnpv genes encoding the pe protein, and orf 1 demonstrated 27 and 34% amino acid sequence identity, respectively, with the co ... | 1992 | 1607868 |
| nucleotide sequence of the p39-capsid gene region of the lymantria dispar nuclear polyhedrosis virus. | a 1.85 kb region, containing an open reading frame (orf) homologous to the baculovirus p39-capsid gene, was sequenced from the lymantria dispar multicapsid nuclear polyhedrosis virus (ldmnpv) genome. analysis of the p39-capsid gene demonstrated that it was 39% and 47% identical in amino acid sequence with the homologous genes in the autographa californica and orgyia pseudotsugata mnpvs, respectively. two late promoter elements located upstream of the p39 gene in the ldmnpv genome are conserved w ... | 1992 | 1607869 |
| genetic engineering of a lymantria dispar nuclear polyhedrosis virus for expression of foreign genes. | a bacterial lacz gene was inserted into an isolate of the lymantria dispar nuclear polyhedrosis virus (ldmnpv). the transfer vector was constructed by site-directed mutagenesis of the translation start site of the ldmnpv polyhedrin gene, within the bglii e fragment of the viral genome. a multiple cloning sequence was inserted at this start site and used for the insertion of the lacz gene into the transfer plasmid. liposome transfection was used to cotransfect l. dispar tissue culture cells with ... | 1992 | 1607870 |
| insecticidal properties of genetically engineered baculoviruses expressing an insect juvenile hormone esterase gene. | exploring the possibility of enhancing the properties of baculoviruses as biological control agents of insect pests, we tested the effect of expressing an insect gene (jhe) encoding juvenile hormone esterase. juvenile hormone esterase inactivates juvenile hormone, which regulates the outcome of an insect molt. a cdna encoding the juvenile hormone esterase of heliothis virescens was inserted into the genome of autographa californica nuclear polyhedrosis virus such that the gene was expressed unde ... | 1992 | 1622228 |
| binding of low concentration of peptide to h-2kd produced in insect cells requires mouse beta 2-microglobulin co-expression. | a recombinant baculovirus expressing the murine class i mhc heavy chain h-2kd cdna under the transcriptional control of autografa californica nuclear polyhedrosis virus (acnpv) polyhedrin promoter has been isolated and used to infect sf9 lepidopteran cells either alone or in association with a previously isolated virus expressing mouse beta 2-microglobulina (beta 2-ma). when infected with the heavy chain-encoding virus alone, h-2kd was produced in a beta 2-m-free conformation detected on the sur ... | 1992 | 1622899 |
| foreign gene expression by a baculovirus vector with an expanded host range. | a nuclear polyhedrosis virus (npv) (baculoviridae)-based gene expression system was improved by dna recombination. the bmn cell line established from bombyx mori and the sf21 cell line established from spodoptera frugiperda are non-permissive for autographa californica multicapsid npv (acmnpv) and b. mori npv (bmnpv) replication, respectively. after cotransfection of acmnpv dna and bamhi-digested bmnpv dna into sf21 cells, progeny viruses were isolated by plaque purification on bmn cell monolaye ... | 1992 | 1629707 |
| characterization of two variants of panolis flammea multiple nucleocapsid nuclear polyhedrosis virus. | the baculovirus panolis flammea multiple nucleocapsid nuclear polyhedrosis virus (pfmnpv) was originally isolated from a natural virus epizootic and shown to consist of a mixture of variants. two subclasses of variants (pfmnpv a and b) were identified by southern blot hybridization, their polyhedrin genes being located on different restriction fragments. the proportion of the a and b variants changed according to the larval host in which the virus was propagated; pfmnpv(b) predominated in p. fla ... | 1992 | 1629708 |
| nucleotide sequence and transcriptional analysis of a gene encoding gp41, a structural glycoprotein of the baculovirus autographa californica nuclear polyhedrosis virus. | we have identified and sequenced a region of the autographa californica nuclear polyhedrosis virus (acmnpv) genome encoding the major polyhedron-derived virus structural glycoprotein gp41. the open reading frame is located entirely within the acmnpv sstii-m fragment. the protein sequence does not have hydrophobic regions characteristic of integral membrane proteins and contains a putative o-linked glcnac glycosylation site. gp41 is expressed as a late gene, with transcripts starting within two c ... | 1992 | 1629955 |
| purification and functional characterization of the human beta 2-adrenergic receptor produced in baculovirus-infected insect cells. | a human cdna fragment bearing the complete coding region for the beta 2-adrenergic receptor was introduced into the genome of autographa california nuclear polyhedrosis virus under the control of the polyhedrin promoter. binding studies using [125i]iodocyanopindolol showed that sf9 insect cells infected with the recombinant virus expressed approximately 1 x 10(6) beta 2-adrenergic receptors on their cell surface. photoaffinity labeling of whole cells and membranes revealed a molecular weight of ... | 1991 | 1645288 |
| secretion of biologically active leech hirudin from baculovirus-infected insect cells. | the thrombin inhibitor, hirudin, from the leech hirudo medicinalis, is the most powerful natural anticoagulant known. it has been characterized as a polypeptide of 65 amino acids which exhibits its anticoagulant properties by binding tightly and specifically to alpha-thrombin. the potency and specificity of hirudin have generated interest on its possible use in the treatment or prophylaxis of various thrombotic diseases. we have used the baculovirus expression system to efficiently produce activ ... | 1991 | 1647362 |
| high level expression of dna polymerases from herpesviruses. | the dna polymerase genes of human cytomegalovirus (hcmv) and varicella-zoster virus (vzv) were inserted separately into the polyhedrin gene of autographa californica nuclear polyhedrosis virus (acnpv) by cotransfection of spodoptera frugiperda (sf9) cells with baculovirus transfer vectors carrying the genes and acnpv infectious dna. infection of sf9 cells with the recombinant viruses resulted in expression from the polyhedrin promoter of proteins of the expected mrs. these proteins possessed dna ... | 1991 | 1649906 |
| insect virus: assays for viral replication and persistence in mammalian cells. | viral pesticidal agents must be evaluated for potential health hazards prior to utilization. assessment of the likelihood of replication in humans has included in vitro exposure of human cells to the potential pesticidal agent. previous in vitro evaluation strategies have lacked positive controls. thus, negative results, interpreted as no effect of the virus on human cells, could reflect basic deficiencies in the testing protocols. we designed a testing scheme for viral pesticides and used it to ... | 1991 | 1650786 |
| functional expression of the human growth factor activatable na+/h+ antiporter (nhe-1) in baculovirus-infected cells. | we constructed a recombinant baculovirus, based on autographa californica nuclear polyhedrosis virus, containing the human na+/h+ antiporter cdna under control of the polyhedrin promoter. when infected with this recombinant baculovirus, the sf9 cell line, derived from spodoptera frugiperda, expresses a fully functional na+/h+ antiporter as measured by the generation of an amiloride-sensitive na+ influx in response to an acid load. the na+/h(+)-exchange activity, not detectable in sf9 cells, emer ... | 1991 | 1654101 |
| processing and antigenicity of entire and anchor-free spike glycoprotein s of coronavirus tgev expressed by recombinant baculovirus. | the s gene of transmissible gastroenteritis virus (tgev) was inserted into the genome of autographa californica nuclear polyhedrosis virus (acnpv) using the transfer plasmid pvl941. infection of sf9 insect cells with the recombinant virus resulted in the synthesis of a 175k polypeptide which was able to trimerize and was transported to the cell surface as is the authentic tgev s protein. despite the lack of complete carbohydrate processing, the recombinant s protein exhibited antigenic propertie ... | 1991 | 1660201 |
| a functional hepatitis b virus x protein produced in insect cells. | we have constructed a recombinant baculovirus from autographa californica nuclear polyhedrosis virus, called acx, that expresses the gene encoding the hepatitis b virus x protein in infected spodoptera frugiperda (sf21ae) insect cells. a 16.5-kda monomer and a 33-kda dimer of the x protein were detected in extracts from acx-infected cells on immunoblots using a polyclonal anti-x antibody. the biological activity of the insect cell-produced x protein was assayed by fusing acx-infected sf21ae cell ... | 1991 | 1660211 |
| characterization of pseudorabies virus neutralization antigen glycoprotein giii produced in insect cells by a baculovirus expression vector. | the gene encoding the complete glycoprotein of pseudorabies virus (prv, yamagata-s81 strain glycoprotein giii) has been inserted into the baculovirus transfer vector pacym1s derived from the nuclear polyhedrosis virus of autographa californica (acnpv). a spodoptera frugiperda cell line, sf21ae, was efficiently co-transfected with the transfer vector containing the giii gene and acnpv dna by cationic liposomes (lipofectin). the gene was placed under the control of the acnpv polyhedrin promoter an ... | 1991 | 1661981 |
| immunoselection of recombinant baculoviruses expressing high levels of biologically active herpes simplex virus type 1 glycoprotein d. | the dna sequence encoding the complete herpes simplex virus type 1 (hsv-1) glycoprotein d (gd) was inserted into a baculovirus transfer vector under control of the polyhedrin gene promoter of the baculovirus autographa california nuclear polyhedrosis virus (acnpv). after co-transfection of spodoptera frugiperda (sf9) insect cells with wild-type acnpv dna and the recombinant transfer vector dna, polyhedrin-negative recombinants that expressed high levels of hsv-1 gd were isolated using immunoaffi ... | 1991 | 1662037 |
| dna restriction polymorphism in wild isolates of spodoptera frugiperda nuclear polyhedrosis virus. | restriction endonuclease analysis was used to examine variation in dna of 22 wild isolates of spodoptera frugiperda nuclear polyhedrosis virus (sfnpv). eleven of the 15 isolated from louisiana were distinguishable based on restriction fragment profiles from the enzymes bamhi, hindiii, and ecori. there was significant genetic variation in sfnpv isolates within single agricultural fields. nucleotide sequence divergence values, based on restriction fragment profiles, indicated that genetic variatio ... | 1991 | 1679452 |
| temporal analysis and spatial mapping of lymantria dispar nuclear polyhedrosis virus transcripts and in vitro translation polypeptides. | genomic expression of the lymantria dispar multinucleocapsid nuclear polyhedrosis virus (ldmnpv) was studied. viral specific transcripts expressed in cell culture at various times from 2 through 72 h postinfection were identified and their genomic origins mapped through northern analysis. sixty-five distinct transcripts were identified in this analysis. most viral transcripts were expressed late in infection, and originated from throughout the viral genome. viral polypeptides expressed in infect ... | 1991 | 1685048 |
| transcription of the baculovirus polyhedrin gene reduces the levels of an antisense transcript initiated downstream. | a late 3.2-kilobase (kb) rna initiated approximately 2 kb downstream of the 3' end of the autographa californica nuclear polyhedrosis virus polyhedrin-coding sequence and traversed the polyhedrin gene in an antisense direction. this rna was sense rna for the two open reading frames flanking the polyhedrin gene. a mutant virus, vxpoly, which differs from wild-type virus only at the essential rna initiation site in the polyhedrin promoter, exhibited higher levels of the 3.2-kb rna than did wild-ty ... | 1990 | 1692351 |
| gene organization and transcription of ted, a lepidopteran retrotransposon integrated within the baculovirus genome. | a single copy of the retrotransposon ted, from the moth trichoplusia ni (a lepidopteran noctuid), was identified within the dna genome of the baculovirus autographa californica nuclear polyhedrosis virus. determination of the complete nucleotide sequence (7,510 base pairs) of the integrated copy indicated that ted belongs to the family of retrotransposons that includes drosophila melanogaster elements 17.6 and gypsy and thus represents the first nondipteran member of this invertebrate group to b ... | 1990 | 1692964 |
| categorizing some early and late transcripts directed by the autographa californica nuclear polyhedrosis virus. | using an s1 mapping assay on rna from spodoptera frugiperda cells infected by the autographa californica nuclear polyhedrosis virus in the presence and absence of cycloheximide and aphidicolin, we can distinguish three classes of transcripts. first, there are those whose synthesis is blocked by the dna synthesis inhibitor aphidicolin and which are therefore late transcripts. these include the late transcript of the 39k gene and a late leftward transcript across the xhoi site in the hindiii-f reg ... | 1990 | 1698929 |
| characterization of virus-like particles produced by a recombinant baculovirus containing the gag gene of the bovine immunodeficiency-like virus. | the entire gag gene of the bovine immunodeficiency-like virus (biv) was inserted behind the strong polyhedron promoter of autographa californica nuclear polyhedrosis virus (acnpv). the resultant recombinant baculovirus (acnpv-bivgag) was used to infect insect cells in order to overexpress and characterize biv gag gene products. the infection resulted in the high-level expression of a protein similar in size to the predicted biv gag precursor (pr53gag). biv pr53gag was detected in acnpv-bivgag-in ... | 1990 | 1699349 |
| the basic dna-binding protein of bombyx mori nuclear polyhedrosis virus: the existence of an additional arginine repeat. | the basic dna-binding protein of the bombyx mori nuclear polyhedrosis virus (bmnpv) was purified by hplc and a sequence of 45 amino acids from the n-terminus was determined. there were no detectable modifications such as n-terminal blockage, glycosylation, or phosphorylation. the amino acid sequence showed high homology to the predicted amino acid sequences of the basic proteins of autographa californica npv (acnpv) and orgyia pseudotsugata npv (opnpv) (90 and 76%, respectively), however, the bm ... | 1991 | 1703373 |
| changes in macromolecular synthesis of gypsy moth cell line iplb-ld652y induced by autographa californica nuclear polyhedrosis virus infection. | the aberrant replication of the autographa californica multiple-enveloped nuclear polyhedrosis virus (acmnpv) in the lymantria dispar cell line iplb-ld652y was used as a model system for the investigation of factors regulating baculovirus host specificity. a previous study of this system indicates that viral gene expression in infected cells is extremely attenuated and subsequently all cellular and viral protein synthesis is inhibited. in the present study, infection of iplb-ld652y cells with ac ... | 1991 | 1709681 |
| enzyme activities in four different forms of human immunodeficiency virus 1 pol gene products. | five cassettes of the pol gene of human immunodeficiency virus 1 were constructed and inserted under the control of the polyhedrin gene promoter of autographa californica nuclear polyhedrosis virus by homologous recombination. the first cassette polf contains the full-length pol open reading frame; the second cassette pol100 starts with the first aug codon of the pol gene and deletes 103 amino acids from the amino terminus of the pol gene product; the third cassette pol97 deletes the entire prot ... | 1991 | 1711203 |
| expression of post-translational processing of preprocecropin a using a baculovirus vector. | a cdna fragment encoding preprocecropin a was inserted into the baculovirus autographa californica nuclear polyhedrosis virus downstream of the polyhedrin promoter. the gene was expressed in recombinant-infected last instar larvae of trichoplusia ni and in diapausing pupae of hyalophora cecropia. the identity of the recombinant product was established by electrophoresis with detection of antibacterial activity and mass spectrometry. the prepropeptide had been correctly processed including remova ... | 1991 | 1712729 |
| construction, purification and characterization of a recombinant baculovirus containing the gene for alpha subunit of human chorionic gonadotropin. | a cdna encoding the alpha subunit of human chorionic gonadotropin, a placental glycoprotein hormone, was cloned downstream to the viral polyhedrin gene promoter of autographa california nuclear polyhedrosis virus and the recombinant transfer vector was used to co-transfect spodoptera frugiperda cells growing in culture. recombinant baculovirus carrying the alpha hcg gene was detected and isolated after dot hybridization using supernatant from co-transfected cells. recombinant vac alpha hcg havin ... | 1991 | 1721605 |
| synthesis and toxicity of full-length and truncated bacterial cryivd mosquitocidal proteins expressed in lepidopteran cells using a baculovirus vector. | full-length (72k) and truncated (61k) cryivd mosquitocidal proteins of bacillus thuringiensis (bt) were expressed in spodoptera frugiperda cells and larvae of trichoplusia ni using a baculovirus vector to investigate the role of cryivd peptides in toxicity as well as to evaluate further the baculovirus/lepidopteran system for expressing bt proteins. the cryivd genes were inserted into the autographa californica multinucleocapsid nuclear polyhedrosis virus (acmnpv) under control of the polyhedrin ... | 1992 | 1730944 |
| transcription analysis of the ecori d region of the baculovirus autographa californica nuclear polyhedrosis virus identifies an early 4-kilobase rna encoding the essential p143 gene. | we have investigated the transcriptional activity of the 60.1- to 68.3-map-unit region of the baculovirus autographa californica nuclear polyhedrosis virus (acmnpv). twelve transcripts mapping to this region were expressed at various times during infection. an early 4.0-kb transcript, potentially coding for a 143-kda peptide essential for viral dna replication, was maximally abundant at 6 h postinfection (p.i.). transcripts of 0.5, 1.1, 1.4, 2.1, and 3.1 kb were most abundant at 12 h p.i., while ... | 1992 | 1731106 |
| the glycoprotein of thogoto virus (a tick-borne orthomyxo-like virus) is related to the baculovirus glycoprotein gp64. | thogoto (tho) virus is a tick-transmitted virus which shares morphological and biochemical characteristics with members of the orthomyxoviridae. the genome of thogoto virus comprises six segments of single-stranded, negative sense rna. the complete nucleotide sequence of the fourth largest rna segment of tho virus has been determined from cdna analyses. this rna segment is 1574 nt long and has a coding capacity for a glycoprotein of 512 amino acids with a predicted molecular weight of 57,550 da. ... | 1992 | 1733105 |
| molecular analysis of the trans-activating ie-2 gene of orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. | a second immediate early (ie) regulatory gene of the baculovirus orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (opmnpv) has been identified. the ie-2 gene which is homologous to the ie-n gene of autographa californica mnpv was mapped to the hindiii a fragment of opmnpv between 0.41 to 1.37 map units. the ie-2 gene codes for a predicted protein of 45,640 da and analysis of the amino acid sequence shows that the protein has a highly basic amino terminal domain and a cysteine-rich dom ... | 1992 | 1736546 |
| tandemly repeated sequence at the 3' end of the ie-2 gene of the baculovirus orgyia pseudotsugata multicapsid nuclear polyhedrosis virus is an enhancer element. | an enhancer element was identified in the virus orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (opmnpv) that is located adjacent to the 3' end of the ie-2 gene and 5' to an open reading frame that codes for a predicted protein that has 37% homology to the acmnpv pe-38 gene. the opmnpv enhancer (ope) consists of a 66-bp element that is tandemly repeated partially or completely 12 times. the ope sequences were shown to increase gene expression from the autographa californica mnpv dela ... | 1992 | 1736547 |
| expression of the mouse c-abl type iv proto-oncogene product in the insect cell baculovirus system. | the cellular gene c-abl is the normal homologue of the transforming gene (v-abl) within the genome of the abelson leukaemia virus. the cdna sequence coding for the cellular form of the murine abl gene (c-abl type iv) has been inserted into the baculovirus transfer vector, pac36c, so that the c-abl gene is under the control of the polyhedrin promoter of autographa californica nuclear polyhedrosis virus (acnpv). spodoptera frugiperda cells infected with the recombinant transfer vector in the prese ... | 1992 | 1737071 |
| baculovirus recombinant expressing a secreted form of a transmembrane carcinoma-associated antigen. | ga733-2 is a monoclonal antibody-defined, 40-kda glycoprotein antigen that is associated with carcinomas of various origins. hydrophobicity analysis of the protein sequence predicted by complementary dna (cdna) has suggested that the ga733-2 antigen is a type i membrane protein. in this study, the polymerase chain reaction was used in a strategy to omit cdna sequences for the transmembrane and cytoplasmic domains, thereby converting the extracellular domain into a secretory protein. full-length ... | 1992 | 1737342 |
| expression and characterization of recombinant mouse beta 2-microglobulin type a in insect cells infected with recombinant baculoviruses. | the murine beta 2-microglobulina cdna was cloned into pac373 and pvl941 transfer vectors and introduced via homologous recombination into the genome of autographa californica nuclear polyhedrosis virus downstream of the polyhedrin promoter. both types of recombinant baculoviruses were isolated and used to infect spodoptera frugiperda (sf9) lepidopteran cells. beta 2m was synthesized at a substantially higher rate in cells infected with the pvl941-derived virus than when the pac373-based virus wa ... | 1991 | 1754712 |
| effect of an autographa californica nuclear polyhedrosis virion component(s) on dna synthesis and growth in several insect cell lines. | thirteen different insect cell lines representing three different orders were infected with autographa californica nuclear polyhedrosis virus (acmnpv) whose genome had been inactivated in situ by photochemical means or by short wave uv irradiation. changes in rates of cellular dna synthesis, as measured by [3h]thymidine incorporation, and cell growth were subsequently measured at various times post infection. seven cell lines exhibited a significant decline in [3h]thymidine incorporation (compar ... | 1991 | 1759911 |
| a baculovirus dual expression vector derived from the autographa californica nuclear polyhedrosis virus polyhedrin and p10 promoters: co-expression of two influenza virus genes in insect cells. | a baculovirus transfer vector, pacuw3, was developed to facilitate the insertion of two influenza virus genes, those encoding the haemagglutinin (ha) and neuraminidase (na) membrane glycoproteins, into the autographa californica nuclear polyhedrosis virus genome in a single cotransfection experiment. the na gene was inserted in place of the polyhedrin coding sequences under the control of the polyhedrin promoter, whereas the ha gene was placed under the control of a copy of the p10 promoter at a ... | 1991 | 1765769 |
| structural comparison of the autographa californica nuclear polyhedrosis virus-induced rna polymerase and the three nuclear rna polymerases from the host, spodoptera frugiperda. | a partial subunit structure has been determined for the novel rna polymerase that is induced in fall armyworm (spodoptera frugiperda) cells upon infection with the autographa californica nuclear polyhedrosis virus (acnpv). the putative structure includes nine polypeptides; the complexity of this structure is in accord with the high sedimentation coefficient (15s) estimated for this enzyme. a comparison of the putative structure of the virus-induced polymerase with those of the three host nuclear ... | 1991 | 1767583 |
| expression of the s1 sub-unit of pertussis toxin using a recombinant baculovirus. | the full-length genome of the s1 sub-unit containing the native signal sequence was inserted downstream of the polyhedrin promoter of autographa californica nuclear polyhedrosis virus. a large amount of 28 kd protein, which was larger than the authentic s1 sub-unit (26 kd), was detected in insect cells infected with the recombinant virus by coomassie blue staining after resolving by sds-page. although the level of expression of the recombinant s1 sub-unit was high, immunoblotting and n-terminal ... | 1991 | 1778337 |
| nucleotide sequence of the gene coding for p40, an occluded virion-specific polypeptide of bombyx mori nuclear polyhedrosis virus. | 1991 | 1783783 | |
| high-level expression and characterization of hepatitis b virus surface antigen in silkworm using a baculovirus vector. | the bombyx mori nuclear polyhedrosis virus (bmnpv) in the silkworm was used successfully for mass production of biologically active foreign genes under the control of the polyhedrin promoter. this system was adapted for the production of large amounts of hepatitis b virus surface antigens (hbsag). the dna fragments coded for the middle protein, which is composed of the s protein with the pre-s2 region, were cloned, the signal protein gene of beta-ifn was added, and both were inserted into a clon ... | 1991 | 1816252 |
| the nonstructural protein (nss) encoded by the ambisense s rna segment of tomato spotted wilt virus is associated with fibrous structures in infected plant cells. | the open reading frame located in the viral strand of the ambisense s rna of tomato spotted wilt virus (tswv), was cloned into transfer vector pac33dz1 and inserted downstream of the polyhedrin promoter in the autographa californica nuclear polyhedrosis virus genome. recombinant baculoviruses were obtained that showed a high-level expression of a 52.4-kda protein corresponding to the inserted tswv gene. the viral protein thus produced was purified and injected into rabbits to raise antibodies. w ... | 1991 | 1826573 |
| secretion of single-chain urokinase-type plasminogen activator from insect cells. | a cdna encoding human urokinase-type plasminogen activator was inserted downstream from the polyhedrin promoter of the baculovirus autographa californica nuclear polyhedrosis virus. a protein of similar mr to urokinase (uk) was synthesized and approx. 90% was secreted from recombinant virus-infected spodoptera frugiperda cells. zymography and western blotting analysis of the insect-derived protein demonstrated that it was comprised solely of the high-mr form of uk. no low-mr uk was detected. ami ... | 1991 | 1834526 |
| field evaluation of a dna hybridization assay for nuclear polyhedrosis virus in gypsy moth (lepidoptera: lymantriidae) larvae. | dna hybridization assays were used to detect the presence of viral dna in gypsy moth (lymantria dispar l.) larvae collected weekly from high density populations or reared from field-collected egg masses. dna was extracted from larvae, bound to nitrocellulose filters, and hybridized with digoxigenin-labeled l. dispar npv (ldnpv) dna probes. the virus incidence determined from dna hybridization assays was compared with that determined with conventional microscopic examination of larvae for polyhed ... | 1991 | 1842802 |
| high-level expression of the epstein-barr virus alkaline deoxyribonuclease using a recombinant baculovirus: application to the diagnosis of nasopharyngeal carcinoma. | the epstein-barr virus (ebv) alkaline deoxyribonuclease (dnase) was inserted into the baculovirus autographa californica nuclear polyhedrosis virus (acmnpv). infection of the insect cell line spodoptera frugiperda (sf9) with the recombinant virus led to the expression of an enzymatically active alkaline dnase. the recombinant ebv alkaline dnase was highly soluble, and the recombinant baculovirus produced approximately 10-20 mg of ebv dnase per 1 x 10(9) cells. the recombinant enzyme activity was ... | 1991 | 1847261 |
| direct double-stranded dna sequencing with baculovirus genomes. | double-stranded dna sequencing with the modified t7 dna polymerase (sequenase) was performed directly with nuclear polyhedrosis virus dna genomes. the conditions were optimized to allow for a rapid and unambiguous sequence analysis of nuclear polyhedrosis virus genomes. | 1991 | 1849913 |
| detection and analysis of autographa californica nuclear polyhedrosis virus mutants with defective interfering properties. | defective interfering particles (dips) were generated upon continuous production of autographa californica nuclear polyhedrosis virus (acnpv) in bioreactors. this configuration mimicked the serial undiluted passaging of virus, which is known to result in plaque-morphology mutants. restriction enzyme analysis of dip-containing preparations of extracellular virus showed the presence of many dna fragments in less than equimolar amounts. these fragments were colinear on the physical map of acnpv and ... | 1991 | 1853572 |
| a baculovirus homolog of a cu/zn superoxide dismutase gene. | a gene with greater than 50% amino acid sequence identity to eukaryotic cu/zn superoxide dismutase genes (sod) was found at 19 map units in the genome of the baculovirus autographa californica nuclear polyhedrosis virus (acmnpv). this gene was transcribed into 1.4- and 1.5-kb rnas late in virus infection. the two late rnas have coterminal 3' ends but initiate from two different start points, both of which map to the central adenine of the sequence motif attaag. the late gene product was found to ... | 1991 | 1871962 |
| dengue-1 virus envelope glycoprotein gene expressed in recombinant baculovirus elicits virus-neutralizing antibody in mice and protects them from virus challenge. | in order to test the feasibility of baculovirus (autographa californica nuclear polyhedrosis virus, acnpv) expression vectors for making immunogens against dengue-1 (den-1) virus, a portion of the envelope (e) glycoprotein gene of den-1 virus was cloned and expressed. the recombinant baculovirus contains 107 nucleotides from the 3' terminus of the den-1 matrix (m) gene, which encodes a hydrophobic signal peptide and extends through the first 1, 245 nucleotides of e, terminating 243 nucleotides b ... | 1991 | 1877710 |
| synthesis of the virus-specified tubules of epizootic haemorrhagic disease virus using a baculovirus expression system. | the formation of virus-specific tubules is one of the most characteristic features in the orbivirus infection cycle, yet little is known about their role in virus replication. the tubuli are composed of a major nonstructural protein, ns1. we have investigated the expression of the ns1-encoding gene of epizootic haemorrhagic disease virus serotype 2 (alberta-strain) by producing a recombinant autographa californica nuclear polyhedrosis virus (acnpv). prior to cloning in the baculovirus transfer v ... | 1991 | 1891959 |
| repeat sequences from complex ds dna viruses can be used as minisatellite probes for dna fingerprinting. | in a search for new fingerprinting probes for use with sheep, repeat sequences derived from five poxviruses, an iridovirus and a baculovirus were screened against dna from sheep pedigrees. probes constructed from portions of the parapox viruses, orf virus and papular stomatitis virus and the baculovirus from the alfalfa looper, autographa californica, nuclear polyhedrosis virus all gave fingerprint patterns. probes from three other poxviruses and an iridovirus did not give useful banding pattern ... | 1991 | 1892249 |
| novel regulatory properties of the ie1 and ie0 transactivators encoded by the baculovirus autographa californica multicapsid nuclear polyhedrosis virus. | the baculovirus autographa californica multicapsid nuclear polyhedrosis virus expresses two immediate-early genes from the hindiii-g region (map units 90.4 to 96.8) of the genome. during the early phase of infection, nonspliced 1.9-kb and spliced 2.1-kb transcripts are expressed which encode the ie1 and ie0 (spliced ie1) gene products, respectively. these two gene products differ only in that ie0 contains an additional 54 amino acids at the amino terminus. rna analysis of these two genes during ... | 1991 | 1895384 |
| expression of human alzheimer amyloid precursor protein in insect cells. | the amyloid beta-peptide is a major constituent of amyloid deposited in the brains of patients with alzheimer's disease and is derived from a larger precursor protein/s (app-695, 751, 770). a human cdna encoding full-length app-751 was inserted into the genome of autographa californica nuclear polyhedrosis virus under transcriptional regulation of the viral polyhedrin gene promoter. the recombinant virus was used to infect insect cells, which resulted in the abundant expression of app-751. analy ... | 1991 | 1899577 |
| synthesis of biologically active adenovirus preterminal protein in insect cells using a baculovirus vector. | a dna fragment encoding the polyhedrin promoter of autographa californica multiple nuclear polyhedrosis virus (acmnpv strain) was constructed using overlapping oligodeoxyribonucleotides (oligos), which included the 5'-untranslated leader sequence of the polyhedrin-encoding gene. this dna fragment was cloned into an intermediate transfer vector (pkx105) providing a unique bamhi site for the insertion of foreign genes. the escherichia coli lacz gene was first cloned at the bamhi site of pkx105 and ... | 1991 | 1905255 |
| alpha-mannosidase-catalyzed trimming of high-mannose glycans in noninfected and baculovirus-infected spodoptera frugiperda cells (iplb-sf-21ae). a possible contributing regulatory mechanism for assembly of complex-type oligosaccharides in infected cells. | incubation of a spodoptera frugiperda (iplb-sf-21ae) cell extract with the oligosaccharide man9glcnac2, the aglucosyl derivative of the glycan that is normally transferred from the dolichol carrier to the relevant asn residue in the nascent protein, results in its trimming to man6glcnac2, an intermediate that is relatively stable to further alpha-d-mannosidase action in these cells. on the other hand, incubation of a similar extract from cells that had been infected for various times with a wild ... | 1991 | 1911772 |
| transcriptional analyses of baculovirus polyhedrin and foreign gene expression relative to baculovirus p10 mrna levels. | comparisons have been made between the p10 and polyhedrin mrna levels recovered from spodoptera frugiperda cells infected with autographa californica nuclear polyhedrosis virus (acnpv). in molar terms and from 18 h post-infection (p.i.), the polyhedrin mrna species increased to levels one and a half times to twice as high as the p10 levels. the influence of the polyhedrin leader sequence on the expression of a foreign gene under the control of the polyhedrin promoter was investigated using a ser ... | 1991 | 1919531 |
| baculovirus gp64 gene expression: analysis of sequences modulating early transcription and transactivation by ie1. | expression of the baculovirus major envelope glycoprotein gene (gp64) is regulated by transcription from both early and late promoters. to characterize the early promoter and identify sequences involved in the regulation of gp64 early transcription, promoter-reporter gene fusions were generated from the orygia pseudotsugata nuclear polyhedrosis virus gp64 promoter and were analyzed by transient expression in uninfected insect cells. for these analyses, 5' deletion mutations were constructed in t ... | 1991 | 1920618 |
| baculovirus-mediated expression of human apolipoprotein e in manduca sexta larvae generates particles that bind to the low density lipoprotein receptor. | human apolipoprotein e (apoe) is a ligand for the low density lipoprotein (ldl) receptor and mediates the catabolism of several classes of lipoprotein particles. binding of apoe to the ldl receptor requires association of apoe with lipid in a vesicle or a lipoprotein particle. because of this requirement, purified apoe or apoe derived directly from bacterial expression systems does not bind to the ldl receptor. to overcome this problem and to facilitate analysis of apoe structure, recombinant ba ... | 1991 | 1924311 |
| nucleotide sequence of the autographa californica nuclear polyhedrosis 9.4 kbp ecori-i and -r (polyhedrin gene) region. | the nucleotide sequence of a 9.4-kbp region including the polyhedrin gene of the c6 strain of the autographa californica nuclear polyhedrosis virus (acmnpv) genome was determined. these data provide a complete description of the ecori-i fragment, which is used to produce transfer vectors for inserting foreign genes into the acmnpv. ten potential open reading frames (orfs) were identified in the complete sequence, on either strand of dna. the largest of these was 1629 nucleotides in length and wa ... | 1991 | 1926775 |
| high-level secretion of the extracellular domain of the human growth hormone receptor using a baculovirus system. | a chemically synthesized gene (hghr-ed) coding for the extracellular domain (ed) of the human growth hormone (hgh) receptor (hghr) was inserted into the genome of autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. spodoptera frugiperda cells infected with the recombinant virus secreted a protein with hgh-binding activity into the medium. the secreted 35-kda protein was purified to near homogeneity. the purified protein exhibited a high binding affinity (kd = 0 ... | 1991 | 1937047 |
| location and nucleotide sequence of the gene encoding the viral enhancing factor of the trichoplusia ni granulosis virus. | the gene encoding the viral enhancing factor (vef) of trichoplusia ni granulosis virus has been cloned from a lambda gt11 expression library, and the complete nucleotide sequence determined. the vef gene encodes a protein with a predicted mr of 104k which does not share homology with any previously reported proteins. a possible promoter is located four nucleotides upstream of the initiation codon and represents a consensus baculovirus late promoter (ataag). this has been confirmed by the identif ... | 1991 | 1940861 |
| selection kinetics during serial cell culture passage of mixtures of wild-type autographa californica nuclear polyhedrosis virus and its recombinant ac360-beta-gal. | detailed analysis of the selection process in serial co-infections of cell cultures by wild-type autographa californica nuclear polyhedrosis virus (acnpv) strain e2 (acnpv/e2) and ac360-beta-gal, a genetically engineered strain, shows that the unaltered strain was clearly dominant even when it initially constituted the minority component in the inoculum. a method of calculating a selection coefficient that quantifies the relative advantage of one strain of virus over the other under specific cul ... | 1991 | 1940862 |
| dna-binding proteins of baculovirus-infected cells during permissive and semipermissive replication. | certain insect cell lines have been shown to be permissive (tn-368) or semipermissive (iplb-ld-652y) for autographa californica nuclear polyhedrosis virus (acmnpv) replication (mcclintock et al., 1986b). in this report dna-binding proteins were identified in such cell:virus systems by hybridizing western blots containing uninfected and infected cell proteins with acmnpv or host dna probes. in the acmnpv-infected tn-368 permissive cell system, 8 virus-induced dna-binding proteins with molecular w ... | 1991 | 1950170 |
| identification of spliced baculovirus rnas expressed late in infection. | previous to this study, the autographa californica multicapsid nuclear polyhedrosis virus (acmnpv) was known to express only one spliced rna (spliced ie1 or ie0). we have conducted an analysis of rna expressed during infection of spodoptera frugiperda cells with acmnpv and have identified a set of five additional spliced rnas expressed late in infection. a reverse transcription-polymerase chain reaction analysis was used to confirm the identification of the ls (late, spliced) rnas. s1 nuclease a ... | 1991 | 1962442 |
| heterologous recombination between autographa californica nuclear polyhedrosis virus dna and foreign dna in non-polyhedrin segments of the viral genome. | we used the expression vector system of autographa californica nuclear polyhedrosis virus (acnpv) and spodoptera frugiperda insect cells to study mechanisms of recombination in insect cells. we concentrated on the isolation and analysis of heterologous recombinants. the e1 region of human adenovirus type 2 (ad2) was inserted into regions of the acnpv genome which lacked apparent homologies to the polyhedrin region. out of a total of 122 recombinant acnpv plaques, which hybridized to ad2 dna in p ... | 1991 | 1962504 |
| expression of rat tyrosine hydroxylase in insect tissue culture cells and purification and characterization of the cloned enzyme. | rat tyrosine hydroxylase has been expressed at high levels in spodoptera frugiperda cells using a baculovirus expression system. a cdna containing the coding region for pc12 tyrosine hydroxylase was inserted into the unique ecori site of the transfer vector pljc8 to yield the recombinant vector pljc9. spodoptera frugiperda cells were then co-infected with pljc9 and wild type autographa californica nuclear polyhedrosis virus. recombinant virus particles containing the cdna for tyrosine hydroxylas ... | 1990 | 1967606 |
| expression of the human multidrug transporter in insect cells by a recombinant baculovirus. | the plasma membrane associated human multidrug resistance (mdr1) gene product, known as the 170-kda p-glycoprotein or the multidrug transporter, acts as an atp-dependent efflux pump for various cytotoxic agents. we expressed recombinant human multidrug transporter in a baculovirus expression system to obtain large quantities and further investigate its structure and mechanism of action. mdr1 cdna was inserted into the genome of the autographa californica nuclear polyhedrosis virus under the cont ... | 1990 | 1970935 |