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the occurrence of parasitic arthropods in two groups of european bison in the białowieza primeval forest.within 1992-2000, a total of 181 białowieza forest bison were examined from two winter herds. twelf parasitic arthropod species were observed, a high infestation being typical of demodex bisonianius, chorioptes bovis, ixodes ricinus, dermacentor reticulatus, and bisonicola sedecimdecembrii. the infestation in section 422 herd being higher for b. sedecimdecembrii, i. ricinus, d. reticulatus, ch. bovis. d. bisonianus was slightly more prevalent in the section 391 herd, the intensity being, however ...200116886430
[is the parasite fauna of poland well recognized?].the studies of parasite fauna have in poland a long tradition. generally the helmint fauna of all groups of vertebrates was more or less examined and as much as over 100 species of monogenea, almost 400 digenea, over 250 cestoda, about 500 nematoda and 32 acanthocephala have been recorded. the best recognized are the helminths of fish (especially those of cyprinidae, esocidae, percidae and salmonidae), frogs examined in various regions of poland, some birds (especially connected with water envir ...200316888930
[forming of gastro-intestinal nematodes fauna of free ranging european bison in bialowieza primeval forest during last 17 years (1984-2001)].28 european bison of both sexes and in different age shot in białowieza primeval forest in january 1984, (10 animals), january 1992 (10 animals) and in january and the beginning of february 2001 (8 animals) have been necropsied. the examined animals in mentioned years were of similar age. there were examined abomasa and duodena of shot animals. all necropsied bison were infected with gastro-intestinal nematodes. the highest intensity of infection with nematodes of abomasum was found in 1992 year ...200216894720
medieval remains from lithuania indicate loss of a mitochondrial haplotype in bison bonasus. 200616911223
normal vaginal micro flora of free-ranging european bison (bison bonasus). 200617071675
application of bovine microsatellite markers for genetic diversity analysis of european bison (bison bonasus).in this study, the cross-amplification of a commercial multiplex set of 11 cattle (bos taurus) microsatellites was tested on a panel of 35 european bison (bison bonasus) individuals. after polymerase chain reaction optimization, all loci cross-amplified successfully in investigated bisons. number of alleles and observed and expected heterozygosity per locus are in the range of 2-4, 0.086-0.629 and 0.288-0.621 respectively. the availability of a heterologous set of multiplexed microsatellite mark ...200617177698
an analysis of inbreeding in the european bison. 196017247924
mhc-drb3 variation in a free-living population of the european bison, bison bonasus.mhc genes play a crucial role in pathogen recognition and are the most polymorphic genes in vertebrates. loss of variation in these genes in bottlenecked species is thought to put their survival at risk. we examined variation at the mhc ii drb3 locus in the european bison, bison bonasus, a species that has undergone an extreme bottleneck: the current population originated from only 12 founders. we also tested for the association of drb3 genes with the incidence of posthitis, a disease affecting ...200717257111
complete genomes and phylogenetic positions of bovine papillomavirus type 8 and a variant type from a european bison.the dna of bovine papillomavirus (bpv) type 8 was extracted from papillomas on cattle kept in japan, and dna of bovine papillomavirus bpv-8-eb was extracted from a european bison (bison bonasus) born in italy and released into the wild in slovakia. the dna genomes of these bpvs were amplified using multiply primed rolling circle amplification and polymerase chain reaction, then characterized by direct sequencing method. the bpv-8 and bpv-8-eb genomes consisted of 7,791 base pairs (bp) and 7,773 ...200717265141
post-thaw viability of european bison (bison bonasus) semen frozen with extenders containing egg yolk or lipids of plant origin and examined with a heterologous in vitro fertilization assay.basic characteristics of european bison (bison bonasus) semen were described and the efficacies of two extenders-triladyl, containing egg yolk, and a synthetic extender, containing soybean lipids-were tested for semen cryopreservation. seven ejaculates were collected by electroejaculation from a 10-yr-old, european bison bull. each ejaculate was diluted at 37 degrees c to a final concentration of 200 x 10(6) sperm/ml with triladyl or the synthetic extender. extended semen samples were frozen acc ...200617312788
seroprevalence of selected viral and bacterial pathogens in free-ranging european bison from the białowieza primeval forest (poland).blood was collected from bison that were selected between 1991 and 2001 for poor body condition, cachexia, lameness and balanoposthitis. sera were tested for antibodies to bovine herpes virus type 1 (bhv-1), bovine viral diarrhoea virus (bvdv), foot-and-mouth disease virus (fmdv), parainfluenza virus type 3 (pi-3), brucella abortus, chlamydophila abortus, coxiella burnetti, and leptospira interrogans. the results of serological tests showed a prevalence of low titers of a serological reaction to ...200717388020
[sequence characterization of the 5'-flanking region of the ghr gene in tibetan sheep].the 5'-flanking sequence (including the p1 promotor and exon 1a) of the ghr gene in oura-type tibetan sheep (o. aries) was cloned by t-a method and sequenced (genbank accession no. ef116490). characterization and comparison of this sequence with mouflons (o. musimon), goat (c. hircus), cattle (b. taurus) and european bison (b. bonasus) orthologues were also conducted. results showed that: 1) the 5'-flanking region contained many potential transcriptional factor binding sites such as those for c/ ...200717681925
the fate of a european bison herd. 189617735251
outbreak of mycobacterium bovis infection in a wild animal park.an outbreak of tuberculosis due to mycobacterium bovis occurred in a wild animal park. three pot-bellied pigs (sus scrofa vittatus), one red deer (cervus elaphus), one buffalo (bison bonasus) and two european lynxes (lynx lynx) were affected and showed clinical signs including weight loss, enlarged lymph nodes and paralysis of the hindlimbs. postmortem examinations revealed multifocal granulomatous lesions in various organs, including the lymph nodes, lungs, intestines, kidneys and the central n ...200717766809
the european bison. 192717787284
proposals for the preservation of the wisent. 192317834061
cellular localisation of the pregnancy-associated glycoprotein family (pags) in the synepitheliochorial placenta of the european bison.this paper describes the cellular immuno-localisation of the pag family in synepitheliochorial (cotyledonary) placenta of the european bison (eb). uteri were harvested from pregnant wild eb (n=4; 45-150 days post coitum-dpc); and additionally from cattle (30, 45 dpc) and pigs (42 dpc)--both domestic species were used as positive controls for cellular pag immunodetection. placentas were sectioned, fixed, dehydrated and subjected to double fluorescent immunohistochemistry (df-ihc) with the use of ...200817854806
some morphological, biochemical and immunological blood parameters in european bison with or without lesions in the digestive, respiratory, urinary and reproductive tracts.ninety-seven bison (39 males and 58 females) were culled out during three successive winter seasons. no infection with bhv-1 and bhv-5 or other viruses was detected by pcr and sn methods. advanced pathological lesions observed in males exhibiting symptoms of posthitis/balanoposthitis are probably due to secondary infection by microorganisms present in the prepuce (corynebacterium spp., bacillus spp., fusobacterium necrophorum, staphylococcus spp. (coagulase negative) and staphylococcus hyicus). ...200717937185
distribution of cryptosporidium and giardia spp. in selected species of protected and game mammals from north-eastern poland.cryptosporidium spp. and giardia spp. are wide-spread pathogens of humans and many species of mammals. the ways of transmission are very complex and difficult to define. both parasites occur in similar environments and share a broad host range. however, in poland there is still little known about the epidemiology of these parasites due to the paucity of data on human cases and only few studies in wildlife. the aim of our study was to determine the distribution of two intestinal protozoa in a few ...200718247463
molecular identification of arcanobacterium bialowiezense and arcanobacterium bonasi based on 16s-23s rrna intergenic spacer region sequences.in the present study, the 16s-23s rdna intergenic spacer region (isr) of arcanobacterium (a.) bialowiezense dsm 17162, a. bonasi dsm 17163, a. bernardiae dsm 9152, a. haemolyticum dsm 20595, a. hippocoleae dsm 15539, a. phocae dsm 10002, a. pluranimalium dsm 13483 and a. pyogenes dsm 20630 was amplified, sequenced and compared with the corresponding 16s rrna gene sequences yielding comparable phylogenetic relationships. the isr sequence of a. bialowiezense and a. bonasi allowed the design of spe ...200818374522
[increase of infection of european bison by nematodes setaria labiatopapillosa in białowie a forest].the aim of these studies was to determine the level of infection of european bison by nematodes belonging to the genus setaria.200718441881
identification of multiple pregnancy-associated glycoproteins (pags) purified from the european bison (eb; bison bonasus l.) placentas.this paper describes the first identified chorionic pags in the european bison (eb), named ebpags, predominantly expressed during early and mid-pregnancy (45-120 day post-coitum; dpc). many ebpags were extracted from various cotyledonary tissues, precipitated, chromatographed (deae and vva: vicia villosa agglutinin), electrophoresed (1d- and 2d-page), analysed by heterologous (cross-species) western blotting and then micro-sequenced by edman degradation. finally, twelve selected vva-purified iso ...200918538513
immunoexpression of aromatase in immature and adult males of the european bison (bison bonasus, linnaeus 1758).based on recent literature dealing with the role of oestrogens in the male gonad, attempts were undertaken to reveal the site of aromatization within the testis of the european bison (bison bonasus). testes were collected from culled animals living in free-ranging populations in bialowieza forest, poland (nine males aged 8 months to 10 years). moreover, to check for any alterations in the expression of testicular aromatase between american bison (bison bison) and european bison, testes from one ...201019032431
characterization and genetic analysis of bovine alpha s1-casein i variant.the aim of this study was to identify the molecular genetic origin underlying the i variant of alpha(s1)-casein and to develop a dna-based test for this polymorphism as a tool for genetic analyses independent of milk sample testing. all coding exons and flanking regions of the alpha(s1)-casein gene were sequenced in dna samples from cattle of known alpha(s1)-casein genotypes (bi, ci, ii, cc), determined by isoelectric focusing of milk samples. a nucleotide substitution (a>t) in exon 11 (g.19836a ...200919392822
deletion/insertion polymorphism of the prion protein gene (prnp) in polish red cattle, polish white-backed cattle and european bison (bison bonasus l., 1758).the aim of the present study was to identify deletion/insertion polymorphism of the bovine prion protein (prnp) gene within the promoter sequence (23 bp indel), intron 1 (12 bp indel) and the 3' end untranslated region (14 bp indel). the experiment was performed on three groups of animals protected under a genetic resources conservation program: 139 polish red (pr) cows, 79 polish white-backed cows and 50 european bison (bison bonasus l., 1758). white-backed cattle were characterized by a higher ...200919507705
the need to rationalize and prioritize threatening processes used to determine threat status in the iucn red list.thorough evaluation has made the international union for conservation of nature (iucn) red list the most widely used and accepted authority on the conservation status of biodiversity. although the system used to determine risk of extinction is rigorously and objectively applied, the list of threatening processes affecting a species is far more subjectively determined and has not had adequate review. i reviewed the threats listed in the iucn red list for randomly selected groups within the three ...200919508675
over-seasonal and sex-independent expression of kappa casein gene (csn3) in mammalian blood lymphocytes.after observing the nonspecific expression of the alpha s1 casein gene in peripheral blood mononuclear cells of goats, we continued to trace illegitimate gene-expression patterns in mammals. six mammalian species (bos taurus, capra hircus, ovis aries, equus caballus, sus scrofa, and bison bonasus) were analyzed for the presence of kappa casein in peripheral blood mononuclear cells and ficoll-isolated lymphocytes. to detect potential relationships between the expression pattern and reproductive s ...200919513825
rickettsia raoultii and anaplasma phagocytophilum in dermacentor reticulatus ticks collected from bialowieza primeval forest european bison (bison bonasus bonasus), poland. 200919548992
[occurrence of coccidia from genus eimeria in european bison in białowieza forest, poland].protozoa from the genus eimeria belong to the least recognized parasites of the european bison. there are only a few data concerning the infection of the bison by these protozoa in białowieza forest. in the winter of 2007/2008 weighting 3 g fecal samples from the rectum of 16 shot bison were examined by flotation method. in 13 of them were found coccidian oocysts from genus eimeria, which were determined to the species by morphometric study under the microscope. in all 13 positive samples, it is ...200919579781
[sarcocystis cruzi (protozoa: apicomplexa: sarcocystiidae) infection in european bison (bison bonasus) from białowieza forest, poland].samples of oesophagus, diaphragm and heart muscles were taken from one european bison from białowieza forest during seasonal european bison elimination in 2008. five gram of each muscle was examined by staining small samples in 0.2% aqueous solution of methylene blue. after that they were placed between compressor glasses and examined under a dissecting microscope in order to detect sarcocystis sp. infection. all sarcocysts observed were counted. the 5-g sample of the heart muscle yielded a tota ...200919579782
divergent evolution in the cytoplasmic domains of prlr and ghr genes in artiodactyla.prolactin receptor (prlr) and growth hormone receptor (ghr) belong to the large superfamily of class 1 cytokine receptors. both of them have been identified as candidate genes affecting key quantitative traits, like growth and reproduction in livestock. we have previously studied the molecular anatomy of the cytoplasmic domain of ghr in different cattle breeds and artiodactyl species. in this study we have analysed the corresponding cytoplasmic signalling region of prlr.200919622175
effectiveness of microsatellite and snp markers for parentage and identity analysis in species with low genetic diversity: the case of european bison.the european bison (bison bonasus) has recovered successfully after a severe bottleneck about 90 years ago but has been left with low genetic variability that may substantially hinder parentage and identity analysis. according to pedigree analysis, over 80% of the genes in the contemporary population descend from just two founder animals and inbreeding coefficients averaged almost 0.5, whereas microsatellite heterozygosity does not exceed 0.3. we present a comparison of the effectiveness of 17 m ...200919623210
age-dependent pattern of connexin43 expression in testes of european bison (bison bonasus, l.).morphology and the expression of connexin43 (cx43) was investigated in testes of immature, prepubertal, and adult european bison bulls by means of routine histology, immunohistochemistry, and western blotting, respectively. testes were collected from culled animals living in bialowieza and borecka forests, poland. histological examination of testicular tissue of immature and prepubertal males revealed normal structure, whereas of adult individuals either normal testicular structure with advanced ...200919623571
ashworthius sidemi (nematoda, trichostrongylidae) in wild ruminants in białowieza forest.between 2003-2007, abomasa of 91 european bison (bison bonasus), 4 red deer (cervus elaphus) and 2 roe deer (capreolus capreolus) shot in the białowieza primeval forest (poland) were examined for worms presence. all the animals examined were infected with nematodes a. sidemi with an exception of one bison, that was shot in year 2003. there was much higher average intensity of invasion in bison (5529), than in red deer (85) and in roe deer (1837). the animals were shot in a period from december t ...200919886261
depauperate genetic variability detected in the american and european bison using genomic techniques.a total of 929 polymorphic snps in eb (out of 54, 000 snps screened using a bovinesnp50 illumina genotyping beadchip), and 1, 524 and 1, 403 polymorphic snps in wb and pb, respectively, were analysed. eb, wb and pb have all undergone recent drastic reductions in population size. accordingly, they exhibited extremely depauperate genomes, deviations from genetic equilibrium and a genome organization consisting of a mosaic of haplotype blocks: regions with low haplotype diversity and high levels of ...200919995416
[non-indigenous and non-specific parasitic nematodes--the background and the consequences].introduction of the host is the main reason behind introduction of the parasite, e. g., nematodes. the introduction of the japanese eel (anguilla japonica) in europe in the 1980s resulted in expansion of the swimbladder nematode anguillicola crassus which soon invaded not only populations of the european eel (a. anguilla), but also other local fish. the raccoon (procyon lotor) is native to north america. it is the specific host of strongyloides procyonis and baylisascaris procyonis. more than 90 ...200920209803
characteristics of the 5' untranslated region of wisent (bison bonasus) and reindeer (rangifer tarandus) pestivirus isolates.the 5'-untranslated region (5'-utr) of the pestivirus strains isolated from wisent and reindeer suffering from severe diarrhoea in the duisburg zoo in germany were amplified by reverse transcription-polymerase chain reaction and sequenced for comparison with those of other pestiviruses from cattle, sheep, goats and swine. phylogenetic trees constructed from the primary nucleotide sequences of these strains demonstrated that the 5'-utr of both the wisent and reindeer isolates were identical and s ...200620429058
in vitro isolation and identification of the first neospora caninum isolate from european bison (bison bonasus bonasus l.).peripheral blood from european bison (bison bonasus bonasus l.) living in białowieża forest, north-east poland, were investigated for the presence of antibodies to neospora caninum and isolation of parasite. out of 23 animals three of them showed a strong positive response to n. caninum (13%). the white blood cells from two positive and two negative bison were loaded on monolayer vero cells culture. the first viable tachyzoites were detected only in positive samples at days 60 and 70 after incub ...201020702042
detection and characterization of bovine-like coronaviruses from four species of zoo ruminants.five coronaviruses (covs) were detected in diarrheal feces from four zoo ruminant species: one wisent (bison bonasus), two himalayan tahr (hemitragus jemlahicus), one sitatunga (tragelaphus spekii), and one nyala (tragelaphus angasii). we sequenced and analyzed the spike (s) and hemagglutinin/esterase (he) genes of these viruses and compared the nucleotide (nt) and deduced amino acid (aa) sequences with those of other bovine cov (bcov) strains. comparison of the entire deduced aa sequences of th ...201020880639
assessment of s100 protein expression in the epididymis of juvenile and adult european bison.in our study, we decided to compare s100 protein expression in the material obtained from the epididymes of 5- and 12-month-old calves, and adult european bison, and to detect any differences in s100 expression according to the animal age and size of the organ examined. we used the epididymes obtained from 6 adult european bison aged 6-12 years, from 6 at the age of 12 months and 6 calves aged 5 months. immunocytochemical reactions were performed using the avidin-biotinylated-peroxidase (abc) te ...201021071335
pathological lesions in european bison (bison bonasus) with infestation by ashworthius sidemi (nematoda, trichostrongylidae).asworthius sidemi schulz, 1933 is a blood sucking gastrointestinal nematode, primarily typical for asiatic deer. it was found for the first time in poland in european bison in 1997. to estimate the level of invasion of a. sidemi and histopathological changes connected with the presence of the parasite in the years 2004-2007 parasitological and histopathological examinations of 54 european bison from białowieza forest were carried out. parasitological examination was carried out by the sedimentat ...201021077432
possibilities of using the european bison (bison bonasus) epididymal spermatozoa collected post-mortem for cryopreservation and artificial insemination: a pilot study.european bison is the largest mammal in europe with the population of approximately 4000 individuals. however, there is no report of post-mortem spermatozoa collection and cryopreservation from this species and the aim of this study was to test if the epididymal spermatozoa collected post-mortem from european bison are suitable for cryopreservation and artificial insemination (ai).201121385402
the annual cycle of shedding eimeria oocysts by european bison (bison bonasus) in the bialowieza primeval forest, poland.abstract between april 2008, and march 2009, we analyzed the pattern of coccidian oocysts present in the feces of the european bison (bison bonasus l., 1758) and found 4 species (eimeria bovis, e. canadensis, e. ellipsoidalis, e. zuernii) previously reported from this host and 3 species (eimeria alabamensis, e. cylindrica, e. pellita), which are new host and locality records. all the species occurred in bison females and only 4 in males; e. bovis was the most prevalent in both sexes. the overall ...201121506851
the state of bioelements in the hair of free-ranging european bisons from białowie a primeval forest.human or animal hair is considered to be a good indicator of the content of bioelements or toxic elements in human and animal organism. its analysis is a good alternative for the arduous obtaining blood or biopsy samples. the aim of the performed research was the assessment of some chosen bioelements in the organism of european bison on the basis of their analysis in hair. the investigation material comprised hair samples obtained from 22 animals. all animals were divided into groups according t ...201121528716
predicting potential european bison habitat across its former range.habitat loss threatens large mammals worldwide, and their survival will depend on habitat in human-dominated landscapes. conservation planners thus face the challenge to identify areas of least conflict with land use, yet broadscale species distribution models rarely incorporate real landscape patterns nor do they identify potential conservation conflicts. an excellent example of such conservation challenges is provided by european bison (bison bonasus). almost extinct by the early 20th century, ...201121639048
is spermiogenesis common or rare in young male european bison aged 2 and 3 years?the aim of the present study was to determine how often spermiogenesis occurs in young male european bison up to 3 years old. research was performed on sections of the testes and epididymes collected from 51 male bison aged 2-3 years. the animals were divided into 2 age groups: young males up to 2 years and young males up to 3 years old, with further separation into specimens with or without spermiogenesis. the animals were culled during the autumn-winter seasons in 1994-2008 (after rutting peri ...201121721408
electrochemical identification of artificial oligonucleotides related to bovine species. potential for identification of species based on mismatches in the mitochondrial cytochrome c1 oxidase gene.our studies show that electrochemical impedance spectroscopy (eis) and scanning electrochemical microscopy (secm) of films of ds-dna on gold allow us to distinguish between mitochondrial dna fragments of the cytochrome c(1) oxidase (mt-cox1) of three related species of the subfamily 'bovinae' (bos taurus, bison bison, and bison bonasus). in eis, a perfectly matched dna gives rise to a considerably larger charge transfer resistance r(ct) compared to mismatched pairings. differences in charge tran ...201121847503
differences in protein binding and cytokine release from monocytes on commercially sourced tissue culture polystyrene.tissue culture polystyrene (tcps) is a ubiquitous substrate used by many researchers in the biomedical and biological sciences. different parameters involved in the production of tcps, including the treatment time and the use of reactive gases and chemical agents, can have a significant influence on the ultimate surface properties achieved. the assumption that they will all yield a consistent and controlled product has not proven to be true. to provide a better insight into the bioactivity diffe ...201221963405
Fecal steroid evaluation to monitor reproductive status in wild ungulate females using enzyme immunoassay commercial kits.Analysis of reproductive hormones in fecal samples is necessary for the noninvasive monitoring of reproductive status in free-ranging species. The aim of the present study was to establish an easy noninvasive method to monitor reproductive status in wild ungulate females. Feces were collected daily, weekly, or three or four times a week directly from the soil for a period ranging from 1 to 9.8 mo. Fecal estradiol and progestagens were monitored in nine wild ungulate females (Barbary sheep, Ammot ...201122204046
tracking of wisent-bison-yak mitochondrial evolution.one of the most informative sources which allow the drawing of far-reaching conclusions about the origins and phylogenetics of many species, including domestic animals and humans, is mitochondrial dna (mtdna). one of the important research targets should include the identification of similarities between wild and domestic species. the analysis involved the nucleotide sequences of mtdna of wisent, auroch, bison, yak, bovine reference sequence (brs) t3, t3a, t3b, t1, t1a, t1'2'3, t2, t3, t4, t5, q ...201222415349
antioxidant enzyme activity and mrna expression in reproductive tract of adult male european bison (bison bonasus, linnaeus 1758).antioxidants in the male reproductive tract are the main defence factors against oxidative stress caused by reactive oxygen species production, which compromises sperm function and male fertility. this study was designed to determine the activity of antioxidant enzymes, such as superoxide dismutase (sod), catalase (cat) and glutathione peroxidase (gpx), in the testicular and epididymidal tissues of adult male european bison (bison bonasus). the reproductive tract tissues were subjected to real-t ...201322458932
detection of mitochondrial dna from domestic cattle in european bison (bison bonasus) from the altai republic in russia. 201222486514
low major histocompatibility complex class i (mhc i) variation in the european bison (bison bonasus).variation in the major histocompatibility complex (mhc) class i of the european bison was characterized in a sample of 99 individuals using both classical cloning/sanger sequencing and 454 pyrosequencing. three common (frequencies: 0.348, 0.328, and 0.283) haplotypes contain 1-3 classical class i loci. a variable and difficult to estimate precisely number of nonclassical transcribed loci, pseudogenes, and/or gene fragments were also found. the presence of additional 2 rare haplotypes (frequency ...201222496313
phenotypic characteristics and virulence genotypes of trueperella (arcanobacterium) pyogenes strains isolated from european bison (bison bonasus).trueperella (arcanobacterium) pyogenes is an opportunistic animal pathogen, which in european bison is associated with different suppurative infections mainly of the urogenital tract. little is known about the virulence of this bacterium and about the pathogenesis of infections. the main objective of this study was to determine phenotypic properties and virulence genotypes of the twenty-five t. pyogenes strains isolated from lesions in various tissues of free-living european bison. classical bac ...201222658663
magnesium concentrations in the tissues of free-ranging european bison.the european bison (bison bonasus) is the only living species of the bison genus in the old world. it is the largest, wild, herbivorous mammal living in europe. as a result of the efforts of biologists, the european bison has been saved as a species. in poland, they were reintroduced into the natural conditions of the bialowieza forest. by 2011, this herd of free-living bison comprised more than 700 animals. the aim of the present work was to determine tissue levels of magnesium in free-living b ...201222728583
farm crops depredation by european bison (bison bonasus) in the vicinity of forest habitats in northeastern poland.european bison, the largest mammal in europe, after being exterminated in the wild and then restored during the 20th century is still listed by the international union for conservation of nature (iucn red list of threatened species) as a species vulnerable to extinction. however, the increasing number of european bison, through creation of new and expansion of existing populations strongly increases the risk of human-bison conflict in the near future. we analyzed the depredation of farm crops by ...201222842710
mitochondrial dna haplotype analysis of liver fluke in bison from bialowieza primaeval forest indicates domestic cattle as the likely source of infection.we have determined the mitochondrial genotype of liver fluke present in bison (bison bonasus) from the herd maintained in the bialowieza national park in order to determine the origin of the infection. our results demonstrated that the infrapopulations present in the bison were genetically diverse and were likely to have been derived from the population present in local cattle. from a consideration of the genetic structure of the liver fluke infrapopulations we conclude that the provision of hay ...201322925823
nematodes of the large intestine of the european bison of the białowieza national park.the present study was conducted on 31 males and females of the european bison, eliminated during the winter seasons 2007-2011 in the białowieza primeval forest, poland. the caeca of 14 free-ranging bison, aged from 3 months up to 16 years, the most favorable infection site for the large intestine nematodes, were investigated during the winter of 2007/2008. the parasitological autopsies of the large intestines of 9 free-ranging bison aged from 5 months up to 10 years as well as 9 bison kept in th ...201223094330
combining multiple autosomal introns for studying shallow phylogeny and taxonomy of laurasiatherian mammals: application to the tribe bovini (cetartiodactyla, bovidae).mitochondrial sequences are widely used for species identification and for studying phylogenetic relationships among closely related species or populations of the same species. however, many studies of mammals have shown that the maternal history of the mitochondrial genome can be discordant with the true evolutionary history of the taxa. in such cases, the analyses of multiple nuclear genes can be more powerful for deciphering interspecific relationships. here, we designed primers for amplifyin ...201323159894
morphometric measurements of the seminiferous tubules of the testes in 2-year-old and 3-year-old european bison males with or without spermiogenesis.the area, perimeter and diameter of the seminiferous tubules in the european bison bison bonasus (l.) were statistically higher in the animals with than in those without spermiogenesis, both among 2-year-old and 3-year-old males (p < 0.001; p < 0.001; p < 0.001).201223214382
in vitro development of bison embryos using interspecies somatic cell nuclear transfer.interspecies somatic cell nuclear transfer (interspecies scnt) has been explored in many domestic and non-domestic animal species. however, problems arise during the development of these embryos, which may be related to species-specific differences in nuclear-cytoplasmic communication. the objectives of this study were to investigate the possibility of producing bison embryos in vitro using interspecies scnt and assess the developmental potential of these embryos. treatment groups consisted of c ...201323692072
co-expression pattern of dopamine beta-hydroxylase (dβh) and neuropeptide y (npy) within sympathetic innervation of ovary and umbilical cord of the european bison (bison bonasus l.).co-expression of dopamine β-hydroxylase (dβh) and neuropeptide y (npy) has never been examined in ovary (ov) and umbilical cord (uc) of the european bison (eb), the endangered wild species. the ov and uc samples were harvested from seasonally eliminated eb females (45-120 days post coitum). frozen histological sections were examined by double fluorescent immunohistochemistry (df-ihc), using the primary mouse anti-dβh monoclonals and rabbit anti-npy polyclonals and then the immunocomplexes were v ...201323928217
malignant catarrhal fever: an emerging disease in the african buffalo (syncerus caffer).within the tribe bovini in the subfamily bovinae, the water buffalo (bubalus bubalis), american bison (bison bison), european bison (bubalus bonasus) and yak (bos grunniens) are recognized as species highly susceptible to malignant catarrhal fever (mcf). in contrast, the lack of reports describing clinical mcf in the african buffalo (syncerus caffer) whether free ranging or captive has led to a perception that african buffaloes are resistant to mcf. during the last decade, several cases of mcf i ...201523957274
first detection of schmallenberg virus in elk (alces alces) indicating infection of wildlife in białowieża national park in poland.schmallenberg virus (sbv) rna was detected in the serum of an elk (alces alces) calf captured on the outskirts of białowieża national park (bnp) in december 2012, and shortly afterwards the calf died of acute bronchopneumonia. serum samples from 169 animals, including bison, red and fallow deer, originating from eight locations situated in four polish provinces, were tested for the presence of sbv-specific antibodies between 2011 and 2013. although no antibodies were found in samples collected u ...201324021421
the arteries of brain base in species of bovini tribe.studies were conducted on 78 preparations of head and brain arteries in four species of bos genus, that is in domestic cattle (n = 59), including 22 foetuses (crl 36.5-78.5 cm), in banteng (bos javanicus, n = 3), yak (bos mutus f. grunniens, n = 2), american bison (bison bison, n = 4), and european bison (bison bonasus, n = 10). the comparative analysis permitted to demonstrate a similar pattern of brain base arteries in the studied animals. in the studied species, blood vessels of the arterial ...201324106047
defecation rate in captive european bison, bison bonasus.data on the digestive characteristics of european bison, bison bonasus (l.), are needed for studies of their role as the largest extant herbivore in europe and a potential keystone species of the temperate forest ecosystem. very little published data are available, particularly on the defecation rate which affects population estimates from dropping counts and also the individual seed deposition rate. we gathered data from a captive bison group kept at the show reserve of the białowieża national ...201324244042
movements of european bison (bison bonasus) beyond the białowieża forest (ne poland): range expansion or partial migrations?the european bison is the largest terrestrial mammal in europe. after extinction in the wild at the beginning of the twentieth century, it was re-introduced to białowieża forest and other woodlands in eastern europe. in this paper, we analysed the movements of european bison beyond the continuous woodlands of the białowieża forest (ne poland) between 1964 and 2010. in total, 1,117 direct observations of bison were collected. the number of males moving out of the forest fluctuated during the stud ...201324244043
the first detection of nematodes ashworthius sidemi in elk alces alces (l.) in poland and remarks of ashworthiosis foci limitations.ashworthius sidemi, a nematode belonging to the family of trichostrongylidae, is a primary parasite of the asian deer, mainly sika deer (cervus nippon), with which it was introduced to ukraine, as well as slovakia, the czech republic and france. migrating red deer carried this parasite from neighboring countries to polish territory. until now, in poland, this parasite has been recorded in european bison, red deer, roe deer and fallow deer. as a result of post-mortem examinations of 10 elk, 2 of ...201324338312
immunocontraception of captive exotic species: v. prolonged antibody titers in dall sheep (ovis dalli dalli) and domestic goats (capra hircus) immunized with porcine zona pellucida.native porcine zona pellucida (pzp) immunocontraception has been used to inhibit fertility in more than 80 species of ungulates, although the duration of contraception efficacy varies among species in both perissodactyla and artiodactyla. this study examined anti-pzp antibody titers in dall sheep and domestic goats at the milwaukee county zoo, and also himalayan tahr and armenian mouflon sheep at the san diego zoo safari park, and, for comparison, altai wapiti, lowland wisent, javan banteng, and ...201324437081
molecular analysis of lungworms from european bison (bison bonasus) on the basis of small subunit ribosomal rna gene (ssu).dictyocaulosis (nematoda: trichostrongyloidea) is a widespread parasitosis of the european bison (bison bonasus) inhabiting bialowieza primeval forest. bearing in mind the current coexistence of bison with wild cervids, and with domestic ruminants in the 19th and 20th century, the need arose for molecular identification of lungworm species. molecular analysis was done on adult lungworms that were obtained from the respiratory track of four free-roaming bison euthanized as a part of the populatio ...201424570058
hepatitis e virus antibody prevalence in wildlife in poland.hepatitis e is an important public health problem mostly in developing but occasionally also in industrialized countries. domestic and wildlife animals are considered reservoirs of the hepatitis e virus (hev). since no information on the prevalence of autochthonous hev infections in human and animal in poland is available, the aim of the study was to investigate the hev seroprevalence of different wildlife species as potential virus reservoirs in the country. no hev antibodies were found in any ...201524655475
the first report of toxoplasma gondii antibodies in free-living european bison (bison bonasus bonasus linnaeus).toxoplasma gondii nicolle et manceaux, 1908 is an apicomplexan parasite with a worldwide distribution. it is of great medical and veterinary importance because it may cause abortion or congenital disease in its intermediate hosts, including man. the european bison, the largest herbivorous animal in europe, is a species that has been saved from extinction. twenty-four of 95 examined sera of the european bison (bison bonasus bonasus) from the białowieza forest, poland collected from 2008 to 2011 w ...201424684049
coccidia (apicomplexa: eimeriidae) of the lowland european bison bison bonasus bonasus (l.).coprological studies conducted between 2007 and 2011 in free-roaming and captive european bison bison bonasus (linnaeus, 1758) from poland revealed 11 species of eimeria infecting the host, i.e., eimeria alabamensis, eimeria auburnensis, eimeria bovis, eimeria brasiliensis, eimeria bukidnonensis, eimeria canadensis, eimeria cylindrica, eimeria ellipsoidalis, eimeria pellita, eimeria subspherica, and eimeria zuernii. the typical host for all isolated species is cattle. the most prevalent species ...201424702772
the usefulness of dna derived from third stage larvae in the detection of ashworthius sidemi infection in european bison, by a simple polymerase chain reaction.ashworthius sidemi, a blood-sucking nematode, is a primary parasite of asiatic cervides, primarily sika deer (cervus nippon). as a. sidemi infections are common in bison, red and roe deer, and gastrointestinal nematodes are often exchanged between animals, it is possible that other farm animals such as cows and sheep that may use the same pastures can be infected. hence, histopathological changes observed in the walls of the abomasa and duodena of infected wildlife caused by a strong parasite pr ...201424886355
migrations and the introduction of wild ruminants as a source of parasite exchange and emergence of new parasitoses.the relationship between european bison and cervidae is a good model for studies on the influence of migration and introduction of new species on the helmhninthofauna of wild ruminants and the occurrence of new parasitoses. changes in the helminthofauna of bison and deer under the influence of changes in the environment and living conditions, as well as the introduction and migration of other species, are discussed in detail. the exchange of helminths between bison, cervids and domestic ruminant ...201424930243
bovine tuberculosis in a wild boar (sus scrofa) in poland.poland is officially tuberculosis free and bovine tuberculosis (btb) cases are rarely found except in bovids. we found btb in a wild boar (sus scrofa) in the bieszczady mountains, southeastern poland. studies suggest possible transmission of infection between free-living european bison (bison bonasus caucasicus) and wild boar in this area.201425075538
study on the occurrence of tick-borne encephalitis virus rna in european bison (bison bonasus) eliminated at białowieza primeval forest (north-eastern poland) in 2005-2009.tick-borne encephalitis virus (tbev) (flaviviridae, flavivirus) is an arthropod-borne virus, an etiologic agent of tick-borne encephalitis (tbe), an infection involving the central nervous system. the disease is endemic in a large region in eurasia where it is transmitted mainly by ixodes ricinus in europe and i. persulcatus ticks in asia. this is the most important tick-transmitted arbovirus of human pathogenicity in europe. the białowieza primeval forest is a well-known endemic focus of tick-b ...201425115061
the parasitic fauna of the european bison (bison bonasus) (linnaeus, 1758) and their impact on the conservation. part 1. the summarising list of parasites noted.during the current century, 88 species of parasites have been recorded in bison bonasus. these are 22 species of protozoa (trypanosoma wrublewskii, t. theileri, giardia sp., sarcocystis cruzi, s. hirsuta, s. hominis, s. fusiformis, neospora caninum, toxoplasma gondii, cryptosporidium sp., eimeria cylindrica, e. subspherica, e. bovis, e. zuernii, e. canadensis, e. ellipsoidalis, e. alabamensis, e. bukidnonensis, e. auburnensis, e. pellita, e. brasiliensis, babesia divergens), 4 trematodes species ...201425119348
the parasitic fauna of the european bison (bison bonasus) (linnaeus, 1758) and their impact on the conservation. part 2. the structure and changes over time.during the last century the recorded parasite fauna of bison bonasus includes 88 species. these are 22 species of protozoa, 4 trematode species, 4 cestode species, 43 nematode species, 7 mites, 4 ixodidae ticks, 1 mallophaga species, 1 anoplura, and 2 hippoboscidae flies. there are few monoxenous parasites, the majority of parasites are typical for other bovidae and cervidae species and many are newly acquired from cervidae. this is an evident increased trend in the parasite species richness, in ...201425119349
studies on eimerians (apicomplexa: eimeriidae) of wild ruminants.the studies were carried out in w. stefanski institute of parasitology polish academy of sciences. doctoral thesis defense took place on may 29th 2012. supervisor: prof. dr hab. aleksander w. demiaszkiewicz.201225165763
persistent müllerian duct syndrome (pmds) in the polish free-ranged bull populations of the european bison (bison bonasus l.).this study describes the diversity of vestigial male uteri of the european bison (eb) examined for: (1) morphology, (2) glycoprotein localization, (3) total protein and glycoprotein profiles, (4) steroid concentrations, and (5) pmds based on the mutation of amh and amhr2 genes. uteri of adult bulls (5-12 years old) were compared to a uterus of a juvenile female (6 months old). male uterine proteins were analyzed in parallel to secretory endometrial proteins of pseudo-pregnant pig (psend) and bsa ...201525498147
cross-sectional study of schmallenberg virus seroprevalence in wild ruminants in poland at the end of the vector season of 2013.in view of recurrent schmallenberg virus (sbv) infections all over europe between 2011 and 2013, a lively scientific debate over the importance of the sylvatic transmission cycle of the virus has emerged. the study presents results of serosurvey which included wild ruminants representing species of red deer (cervus elaphus), roe deer (capreolus capreolus), european bison (bison bonasus), fallow deer (dama dama), mouflon (ovis orientalis musimon) hunted or immobilized at 34 different locations of ...201425528665
genome-wide association study for posthitis in the free-living population of european bison (bison bonasus).about 5-6% of the european bison (bison bonasus) males are affected by posthitis (necrotic inflammation of the prepuce) and die in the wild forest. despite many years of study, pathogenesis of this disease has not yet been determined. the main aim of the study was to find snp markers significantly associated with the incidence of posthitis and mine the genome for candidate genes potentially involved in the development of the disease.201525585689
detection of borrelia burgdorferi sensu lato dna in the blood of wild bison from białowieza primeval forest in eastern poland.the aim of the present study was to investigate the occurrence of borrelia burgdorferi sensu lato dna in a group of 120 wild bison (bison bonasus) from the bialowieza primeval forest in eastern poland. the pcr technique revealed the presence of 16s rna of borrelia burgdorferi sensu lato in the blood of 16 out of 120 examined animals. dna amplification by means of primers sc1 and sc2 gave a product with a size of 300-bp. the sequences of the pcr products obtained showed 100% homology with each ot ...201425638986
serological studies to determine the occurrence of johne's disease and mycoplasma infection in the northern-east polish population of european bison (bison bonasus).a serological study of twenty three european bison (bison bonasus) derived from northern-east poland for the seroprevalence of mycobacterium avium subsp. paratuberculosis, mycoplasma bovis, mycoplasma mycoides subsp. mycoides sc, mycoplasma agalactiae and mycoplasma capricolum subsp. capripneumoniae was conducted. only specific antibodies to m. bovis were detected in two animals (8.7%) which were connected with the clinical signs and macroscopic anatomopathological lesions.201425638988
european bison as a refugee species? evidence from isotopic data on early holocene bison and other large herbivores in northern europe.according to the refugee species concept, increasing replacement of open steppe by forest cover after the last glacial period and human pressure had together forced european bison (bison bonasus)--the largest extant terrestrial mammal of europe--into forests as a refuge habitat. the consequent decreased fitness and population density led to the gradual extinction of the species. understanding the pre-refugee ecology of the species may help its conservation management and ensure its long time sur ...201525671634
dominance of dermacentor reticulatus over ixodes ricinus (ixodidae) on livestock, companion animals and wild ruminants in eastern and central poland.the most common tick species parasitizing animals in poland are ixodes ricinus and dermacentor reticulatus. these tick species differ in their distribution, habitats, seasonal activity and host specificity. ixodes ricinus is the most prevalent and widely distributed, whereas the range of d. reticulatus is limited to eastern and central parts of the country with several new foci in the middle-west and the west. however, as in many central european countries, the range of d. reticulatus is expandi ...201525717007
observations on sporulation of eimeria bovis (apicomplexa: eimeriidae) from the european bison bison bonasus: effect of temperature and potassium dichromate solution.the present study was designed to determine the influence of temperature and the addition of potassium dichromate solution (k2cr2o7) on the efficiency of sporulation of eimeria bovis (zublin, 1908), a coccidian species most commonly diagnosed in european bison bison bonasus (linnaeus). sporulation under conventional conditions (2.5% dichromate solution, 23 °c) was completed on the fourth day of incubation (control), whereas the oocyst development took two days more at the temperature of 18 °c, a ...201525960564
sequence analysis of bitter taste receptor gene repertoires in different ruminant species.bitter taste has been extensively studied in mammalian species and is associated with sensitivity to toxins and with food choices that avoid dangerous substances in the diet. at the molecular level, bitter compounds are sensed by bitter taste receptor proteins (t2r) present at the surface of taste receptor cells in the gustatory papillae. our work aims at exploring the phylogenetic relationships of t2r gene sequences within different ruminant species. to accomplish this goal, we gathered a colle ...201526061084
development of a multiplex pcr for identification of dictyocaulus lungworms in domestic and wild ruminants.dictyocaulus lungworms are the causative agents of parasitic bronchitis (dictyocaulosis) characterised by coughing and severe lung pathology in domestic and wild ruminants. the objective of this study was to design a simple molecular test that could detect of lungworm dna from both adult and larval lungworms and could distinguish between the most common dictyocaulus species found in cattle and in some species of wild ruminants. a multiplex pcr test with four novel primers targeting species-speci ...201526266883
the occurrence of anaplasma phagocyto- philum in wild bison from the bialowieza primeval forest in eastern poland.the aim of the present study was to investigate the occurrence of anaplasma spp. in a group of 120 wild bison (bison bonasus) from the bialowieza primeval forest in eastern poland and to determine which species of anaplasma could infect these animals based on a pcr of a part of the 16s rrna gene followed by sequencing. the pcr technique showed the presence of 16s rrna anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. dna amplification by means of the primers ehr 521 ...201526281444
the prion protein gene polymorphisms associated with bovine spongiform encephalopathy susceptibility differ significantly between cattle and buffalo.prion protein, encoded by the prion protein gene (prnp), plays a crucial role in the pathogenesis of transmissible spongiform encephalopathies (tses). several polymorphisms within the prnp are known to be associated with influencing bovine spongiform encephalopathy (bse) susceptibility in cattle, namely two insertion/deletion (indel) polymorphisms (a 23-bp indel in the putative promoter and a 12-bp indel in intron 1), the number of octapeptide repeats (octarepeats) present in coding sequence (cd ...201526319996
the infection of reintroduced ruminants - bison bonasus and alces alces - with anaplasma phagocytophilum in northern poland.the north-eastern part of poland is considered an area of high risk for infection with tick-borne diseases, including with human granulocytic ehrlichiosis (hge) agents. the etiological agent of hge is anaplasma phagocytophilum. as the animal reservoir for a. phagocytophilum in the environment serve the species from cervidae and bovidae families. european bison (bison bonasus) and elk (alces alces) are the big ruminant species, reintroduced to the forests of middle europe after many decades of ab ...201526408585
[wild mammals of the grand duchy of lithuania in the works of jean-emmanuel gilibert].among the many topics of lively scientific work that jean emmanuel gilibert (1741-1814) conducted in grodno and vilnius, an important place is occupied by his observations of wild mammals. royal patronage and care from antoni tyzenhauz, treasurer of the grand duchy of lithuania and the governor of grodno, allowed gilibert to keep and observe wild fauna captured by royal services in royal forests, including białowieża primeval forest. such was an origin of a female bison kept by gilibert in grodn ...201526445747
first steps into the wild - exploration behavior of european bison after the first reintroduction in western europe.biodiversity is rapidly declining globally. one strategy to help to conserve species is to breed species in captivity and release them into suitable habitats. the way that reintroduced animals explore new habitats and/or disperse from the release site is rarely studied in detail and represents key information for the success of reintroduction projects. the european bison (bison bonasus l. 1758) was the largest surviving herbivore of the post-glacial megafauna in europe before it became extinct i ...201526605549
coronary arteries of the european bison (bison bonasus).the european bison (bison bonasus) is an endangered species. more information on its anatomy is needed as only few studies have been published. this study is the first report on the morphology of the coronary vessels. given the anatomical similarity between the european bison and other ruminants, the results of this study can be applied to other species, including endangered ones.201526608615
the first detection of the tick-borne encephalitis virus (tbev) rna in dermacentor reticulatus ticks collected from the lowland european bison (bison bonasus bonasus l.).tick borne encephalitis virus (tbev) (flaviviridae, flavivirus) is the causative agent of tick-borne encephalitis (tbe), a potentially fatal neurological infection. the disease is endemic in a large region in eurasia, where is transmitted mainly by hard ticks: ixodes ricinus and i. persulcatus. it is known that also dermacentor reticulatus is involved in a circulation of tbev, but the knowledge of its importance in the tbe epidemiology is still insufficient. the białowieża primeval forest is loc ...201626751883
a serological and molecular study on the occurrence of mycoplasmas in european bison (bison bonasus) from two areas of eastern poland.european bison (bison bonasus) from two different areas of eastern poland showing gross pathology possibly associated with mycoplasma infections were tested for ruminant mycoplasma species using serological and molecular methods. fifty-five samples, blood or tissue were collected from 28 animals during 2013-2014. six sera were positive for mycoplasma bovis. the elisa and complement fixation test for mycoplasma mycoides subsp. mycoides gave a few weak reactions, but were negative by immunoblottin ...201526812834
space use and movement patterns in a semi-free-ranging herd of european bison (bison bonasus).the successful reintroduction and restocking of the european bison demands a reliable knowledge of the biology of this species. yet little is known to date about the european bison, and empirical data remains insufficient to set up a reliable plan ensuring the reintroduction, maintenance and survival of populations in habitats that have been largely modified by human activity. studies of the ecology, social behaviour and management of bison are therefore crucial to the conservation of this speci ...201626841107
assessing potential habitat and carrying capacity for reintroduction of plains bison (bison bison bison) in banff national park.interest in bison (bison bison, b. bonasus) conservation and restoration continues to grow globally. in canada, plains bison (b. b. bison) are threatened, occupying less than 0.5% of their former range. the largest threat to their recovery is the lack of habitat in which they are considered compatible with current land uses. fences and direct management make range expansion by most bison impossible. reintroduction of bison into previously occupied areas that remain suitable, therefore, is critic ...201626910226
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