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[fiber-type morphology and function of the triads in frog (rana esculenta) skeletal muscle)].1. owing to differing structural characteristics of the contractile substance, the muscle fibres have been divided into the three types a, b and c in former papers. this distinction seems to be corroborated by our investigations into the different structure regarding the traids. as for the a-fibres, they are structured in terms of the t-system being connected in its entire length with the sr-cisternae and circling the myofibrils at the level of the z-layer. in the b-fibres, this permanent coupin ...1979316237
localization of acid phosphatase in the suprarenal gland of rana esculenta. 2000316356
experimental gynogenesis provides evidence of hybridogenetic reproduction in the rana esculenta complex.the gynogenetic offspring of the hybrid frog rana esculenta (r. ridibunda x r. lessonae) are exclusively of the ridibunda type. this is due to the premeiotic exclusion of the lessonae genome from the hybrid's germ cells.1979316396
[effect of sits (4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid) on the transport of chloride through the isolated skin of rana esculenta].the effect of sits (6 . 10(-4)m on transepithelial potential (e) and short circuit current (i) of isolated frog skin has been studied either using ringer or cl- -depleted ringer in the outside bathing solution. sits affects the transepithelial potential (e) and the short-circuit current (i) only if normal ringer is used and sits is added in the outside bathing medium. the existence of a negative exchanging-charge carrier on the external membrane of the epithelial cells is postulated. this mechan ...1997316702
characteristics of slow potentials from the frog epiphysis (rana esculenta); possible mass photoreceptor potentials. 1999316944
the variety of synaptic vesicles in the interpeduncular nucleus (itp) of the frog.the electron microscope has revealed a large variety of synaptic vesicles in the interpeduncular nucleus (itp) of the frog "rana esculenta". they vary in shape, size and electron density. there are two types of synapses which show only translucent spherical vesicles: in one type the vesicles are 40 nm, in the other type they are 70 nm in diameter. in other types of synapses the translucent vesicles may be mixed with those with dense core. large granules, 160 nm in diameter, already reported in t ...1999316948
morphologic observations on the interrenal stilling and intermediary cells in the adrenal gland of rana esculenta complex. 2015317929
the stilling cells of the adrenal gland of rana esculenta complex during embryonal development and metamorphosis. 2015317974
[morphological and quantitative study of induced sex reversal in rana esculenta tadpoles]. 1979317989
[active transport of chloride across skin isolated from rana esculenta].the effect of sits, ouabain and acetazolamide on potential difference (e) and short-circuit current (ccc) across isolated frog skin after amiloride treatment have been investigated. it has been found that amiloride (3.5 . 10(-5)m) reverses the e and ccc values. after amiloride treatment, with the use of sits (6 . 10(-4)m) positive values of e and ccc have been measured while ouabain (10(-4)m) only slightly reduces the reversed e and ccc values. on the other hand the effect of acetazolamide (6 . ...1979317994
the variation of characteristics of twitch and tetanic contractions with sarcomere length in isolated muscle fibres of the frog.the relation between sarcomere length, tension and time course of tension development in twitch and tetanic contractions at 20 degrees c was determined for isolated fibres from the semitendinosus muscle of the frog (rana esculenta). in twenty fibres at about 2.15 micron sarcomere length, the peak twitch tension, the maximum tetanic tension and the twitch/tetanus ratio ranged, respectively, from 0.22 to 1.6 kg/cm2, from 2.13 o 3.96 kg/cm2 an from 0.07 to 0.53. the peak twitch tension was found to ...1979318017
membrane water permeability alterations induced by pollutants.lead ions and the surfactant nonylphenolethoxylate are found to inhibit the active transport-coupled water flow in the leg skin of rana esculenta. the water permeability for osmotically driven flows remains unchanged for high flows, while it is remarkable lowered near zero osmotic pressure difference. the possible mechanism of the permeability change is discussed in connection with the available data on the electrophysiological effects of the pollutants.1979318022
comparative studies on the nonprotein but nondialysable sugar-peptide fraction isolated from the plasma of various classes of vertebrates.1. the nonprotein but nondialysable ninhydrin-positive substances isolated from plasma of some species belonging to four classes of vertebrates (fishes, amphibians, birds and mammals) were studied. 2. in all species studied (cyprinus carpio, salmo gairdneri, rana esculenta, rana temporaria, gallus domestica and rattus rattus) a similar group of nondialysable ninhydrin-positive substances may be isolated from the plasma by sephadex g-25 filtration followed by dialysis. 3. thin-layer sephadex g-25 ...1977318251
parvalbumins from frog skeletal muscle (rana temporaria l.). isolation and characterization. structural modifications associated with calcium binding.two parvalbumins have been isolated from the skeletal muscle of rana temporaria l. amino acid composition, tryptic peptide maps, isoelectric points, calcium content and ultraviolet as well as circular dichroism spectra have been determined. investigation on antigenic properties revealed no antigenic determinants common to both components. the two protein molecules appear to belong to far related gene lineages. they are also different from the parvalbumins found in rana esculenta muscle. modifica ...1977405049
[superficial potential differences in rana esculenta]. 1977409513
[binding of corticosteroids to plasma proteins in the adult green frog, in various physiological conditions].binding of corticosterone and aldosterone to plasma proteins of the edible frog was studied by equilibrium dialysis technique. a high-affinity, transcortin-like, corticosterone binding system has been demonstrated, but, in vivo, the unbound fraction of this corticosteroid is higher that the bound one. hypophysectomy and thyroidectomy do not change corticosteroids binding parameters which are modified by a variation of temperature.1977409548
[origin of the differences of superficial potentials in rana esculenta].the spatial distributions of superficial d.c. potentials on the skin of rana esculenta have been compared to those of the intensity of short-circuit current (s.c.c.) expressing the transcutaneous active transport of sodium ions. it has been observed that the sites of maximum d.c. potentials coincide with the localisations of maximum s.c.c. values. moreover, superficial d.c. potentials and s.c.c. are similarly modified by the depression of metabolic activity due to lowered temperature or poisonin ...1977409557
effects of two synaptic activators, calcium and ethanol, on mepp distribution in time.miniature end-plate potentials (mepps) were recorded intracellularly from sartorius muscle of rana esculenta. tracings were divided into time bins whose duration approximated one-fifth of the mean interval between consecutive potentials. the observed number of bins containing 0, 1, 2, ... mepps was compared, by the x2 test, with the number calculated from the poisson equation. mepp timing was analyzed in the absence as well in the presence of ca2+ (1 mm, 2.5 mm, and 15 mm). in half of the experi ...1979495743
[life cycle of the trematode echinostoma caproni richard, 1964 (echinostomatoidea) (author's transl)].the life cycle of the trematode echinostomatoidea echinostoma caproni richard, 1964, develops under natural conditions in madagascar in a bird falco newtoni and in a gastropod biomphalaria pfeifferi. metacercaries encyst in other molluscs and in the kidney of an amphibian ptychadaena mascareniensis. the cycle has been reproduced experimentaly in france. the defivitive hosts are the white mouse, the rat and the chick. biomphalaria galbrata is the first and the second intermediate host. metacercar ...1978697289
[adrenal and plasma catecholamines levels in the edible frog under various physiological conditions (proceedings) (author's transl)]. 1978718124
[characteristics of energy metabolism in tissues of edible frog in different seasons].the lowest content of glycogen (2540 mg%) in the vigil frog liver tissue is found in march, soon after hibernation, the maximum content (8000 mg%) is marked in november, before hibernation. in autumn the highest level of nonesterified fatty acids is observed in blood (0.33 muequiv./ml), that of creatine phosphate (64.3 mg%) and atp (66.6 mg%) in the brain tissues. in december-january, in the period of a week an one-and-a-half month hibernation (2-4 degrees c) the content of glycogen in the liver ...2015749297
the influence of dopamine on active sodium transport across frog skin measured as short circuit current.the influence of dopamine (da) on short circuit current (scc) across isolated rana esculenta skin was studied to determine whethera a da receptor system exists in that cell membrane model and what is the influence of da on scc. experiments were carried out with both alpha- and beta-adrenergic blockers and cocaine present in the ringer solution. da in cumulative doses added to the inner ringer solution stimulated scc in a dose-dependent manner. apomorphine (10(-5) m) shifted the da dose--response ...1979759194
immunofluorescence studies on gonadotropin releasing hormone (grh) in the fore-brain and the neurohypophysis of the green frog, rana esculenta l.using antibodies against mammalian lh-rh, the double antibody-immunofluorecence technique has been applied to serial cross sections of the brains of adult rana esculenta. immunoreactive material was found in perikarya of an unpaired nucleus in front of the preoptic recess. the axons of these perikarya also contain fluorescing material. they form a single bundle which passes under the preoptic recess, than splits into two tracts, one on either side of the optic chiasm. the two tracts reunite just ...1976776409
a modification of the rapid golgi method.an extremely rapid golgi procedure for immersion impregnation of the whole brain of the frog rana esculenta is described. it uses an isotonic 2% osmium tetroxide solution buffered with sodium barbital at ph 7.2 that has previously been used for electron microscopy. to 15 ml of this solution 85 ml 3% potassium dichromate is added just before use. twelve-hour treatments (6 hr in the chrome-osmium solution plus 6 hr in 0.75% silver nitrate) are sufficient to obtain consistently excellent impregnati ...1976779149
ultrastructural localization of adenyl cyclase activity in the pancreas of two amphibian species (salamandra salamandra l. and rana esculenta l.).the distribution of adenyl cyclase activity in the pancreas of one species of urodeles and anura has been investigated using electron microscopy. in both islet and acinar tissues the reaction product was located at the outer cell surfaces, while in nerve bundles it occurred in the cleft between axolemma and schwann cell-extensions. the functional signification of the enzyme localization is discussed in relation to the findings of other authors.1976791901
[concentrating ability of the gallbladder: a comparative study in some vertebrate species (author's transl)].the bile salts, chloride, sodium, potassium and nitrogen content of the gallbladder bile was studied in goat, sheep, cattle, pig, rabbit, dog, monkey (erythrocebus patas), chicken, lizard (lacerta ocellata), frog (rana esculenta), trout (salmo gardnieri) and barbel (barbus barbus). a significant correlation was found between bile salts and sodium (positive), chloride and sodium (negative) and chloride and bile salts (negative). the gallbladder concentrating ability was very high in monkey, lizar ...1975808839
[isolation of active gonadotropin fractions from sturgeon hypophysis and procurement of specific immune sera to them].the proteins of sturgeon hypophysis form 12 fractions when being separated by disc-electrophoresis. the maturation of the rana esculenta or bufo viridis oocytes in vitro served as a criterion of gonadotropic activity of fractions. the gonadotropic activity was shown to reside in two glycoprotein fractions with the mobilities 0.26 and 0.34. the immune serum to the former fraction is monospecific under immunodiffusion and suppresses the oocyte maturation in vitro. the immune serum to the latter fr ...1975814521
[optical property changes during activity of myocardial trabeculae and skeletal muscle of rana esculenta].the contractile activity,measured on a small portion of myocardial trabeculae or skeletal muscle, is accompanied by optical changes, analyzed using a signal averaging technique. scattering changes correspond mainly to structural modifications (volume); absorption changes are attributed to early metabolic events while birefringence changes are related to variations in mechanical tension.1975815054
[a molecular hybrid having gonadotropic activity in frogs composed of the alpha subunit of bovine luteinizing hormone and a subunit of a gonadotropin of a teleost fish].the alpha subunit of bovine lh and the subunit su ii of carp gonadotropin are able to associate. the hybrid is about one hundred times less active than c-gth or b-lh, respectively on goldfish and rat ovarian adenyl cyclase. however it is more efficient than both horomones on an amphibian (rana esculenta), on spermiation in vivo as well as on ovarian adenyl cyclase in vitro.1976816536
[inhibition of the sodium inactivation of the nodal membrane by anemonia sulcata toxin ii].a neurotoxin (atx-ii) extracted from the tentacles of anemonia sulcata has been found to interact with the sodium channel of the nodal membrane in myelinated nerve fibres from rana esculenta. if externally applied at low concentration (kd = 20 mum), it reduces considerably the rate of inactivation of the sodium conductance without affecting the activation. at such concentrations, the potassium conductance is not affected. if internally applied atx-ii does not affect the membrane conductance.1976821659
[melanotropic hormone in the green frog (rana esculenta): biochemical and radioimmunological study].the alpha-msh levels in the frog hypophysis were measured by use of a sensitive ans specific radioimmunoassay. sephadex g-50 gel chromatography of frog pars distalis and pars intermedia extracts showed that frog hypophyseal msh was chemically related to synthetic alpha-msh. rat tissue homogenates were capable of degrading frog pars intermedia msh as well as synthetic alpha-msh. these results provide evidence that the frog hypophysis--pars intermedia and pars distalis--contains an alpha-msh-like ...1976827375
noradrenaline induced secretion of nonelectrolytes through frog skin.addition of noradrenaline (4 x 10(-5) m) to the inner bathing fluid in the skin of the frog rana esculenta results in increased unidirectional fluxes of urea, thiourea, n-methyl-thiourea, n-n'-dimethylthiourea and mannitol. fluxes towards the external medium (phi o) undergo a much greater increase than those moving the opposite direction (phi i). the effect of noradrenaline on phi o is higher for urea and thiourea than mannitol, while its effect on phi o thiourea derivatives is related to lipid ...1977894705
early receptor potential from the pineal photoreceptor.rapid photoresponses were obtained from the pineal organ of rana catesbeiana and rana esculenta comparable to the early receptor potential (erp) of the retina of their lateral eyes. light source was a xenon filled discharge tube with a maximum energy input of 365 j. the flash duration was 600 mu sec measured at 1/3 peak amplitude. the response pattern of the erp of the exposed epiphysis cerbri (intracranial portion of the frog's pineal organ) resembled the erp of the isolated retina and the r1 d ...19751078725
chloride transport through the n onshort-circuited isolated skin of rana esculenta.the effects of acetazolamide (diamox), ouabain, and amiloride on chloride movements through the isolated, nonshort-circuited rana esculenta skin were investigated at different external nacl concentrations. from hyposmotic solutions of low nacl concentration (2 mm), two principal components of the chloride uptake may be distinguished: one dependent on the osmotic water flux and the other inhibited by diamox but not by ouabain, amiloride, or removal of sodium from the external solution. chloride u ...19751078757
influence of ambient temperature on growth rate of reissner's fibre in rana esculenta.the influences of different environmental temperatures on the growth rate of reissner's fibre (rf) in rana esculenta were studied in october. at 24 degrees c about 70% and at 18 degrees c about 45% of the length of rf was renewed in 24 hours. this means that the growth rate of rf at 24 degrees c was about 50% faster than that at 18 degrees c. it strongly indicates that increase in ambient temperature from 18 degrees c to 24 degrees c stimulates the secretory activity of the sco considerably at l ...19751078993
a possible functional relationship between the subcommissural organ and the pineal complex and lateral eyes in rana esculenta and rana temporaria.in previous publications (diederen, 1972, 1973) evidence was presented that light and darkness hardly influence the secretory activity of the subcommissural organ (sco) in intact frogs, i.e. frogs in which all photo-receptive organs are present. in the present study the influence of long daily photoperiods and of continuous darkness on the sco of frogs in which the frontal organ, the pineal organ and the eyes were eliminated separately or in combination was studied. the parameters used to measur ...19751080077
hydro-osmotic activity of "carba' analogues of oxytocin and [8-arginine] vasopressin on frog (rana esculenta) bladder.the biological potency of neurohypophysial hormone analogues in stimulating water transport across the frog bladder (rana esculenta) was estimated from the pd2 (negative logarithm of the peptide concentration eliciting 50% of its maximal hydroosmotic effect) and from the maximal response. most analogues elicited maximal responses similar to those of the natural hormones. both replacement of sulfur by a methylene group in the disulfide bridge and omission of the terminal amino group in oxytocin r ...20141080112
chloride transport in isolated skin of rana esculenta.using a modified ussing chamber, transepithelial cl transport in isolated skin of rana esculenta was determined throughout the year. unidirectional 36-cl fluxes were measured in the absence of electrochemical gradients across the skin. the correlations between unidirectional cl influx and cl efflux respectively and the cl concentration in na containing solutions were not linear in the 0 to 90 meq/1 range. the net cl influx calculated from these two correlations was, in the same concentration ran ...19751080271
late sodium current in the node of ranvier.voltage clamp experiments carried out on nodes of ranvier of myelinated fibres of rana esculenta showed that a small fractoon of sodium channels fail to inactivate. thus during long lasting depolarizing pulses there is a small na-current superimposed on the leakage and potassium currents. this late na-current appears more marked in sensory fibres than in motor ones.19921080274
influence of receptor-receptor fibres on the spontaneous afferent activity from semicircular canals in the frog (rana esculenta).the influence of the efferent vestibular system having been eliminated, the spontaneous activity of afferent fibres of the ampullary nerves of the horizontal and vertical anterior semicircular canals was recorded in the frog. by stimulating the ampullary nerve of one of these two canals while recording on the other, as well as by sectioning branches of the vestibular nerve and then using statistical methods, it is shown that receptor-receptor fibres have an inhibitory action on afferent activity ...19751080562
the amino-acid sequence of the most acidic major parvalbumin from frog muscle.the primary structure of the most acidic (pi - 4.50) of the two major parvalbumins from frog (rana esculenta) has been determined from a study of its trypsic peptides and of overlapping peptides generated by limited trypsic digestion, chymotrypsic digestion and n-bromosuccinimide cleavage of the protein. the amino acid sequence so obtained is considered in comparison with those known for other parvalbumins and for rabbit troponin-c.19751080707
effects of furosemide on sodium content and transport pool in frog skin (rana esculenta): comparison with vasopressin and ouabain.the effects of ouabain, vasopressin, and furosemide on intracellular concentrations of total sodium([na]) and potassium [k]), on exchangeable sodium ([na]) and the sodium transport pool ([nap]) were investigated in isolated short circuited skins of rana esculenta. furosemide was added to the epithelial bathing solution, vasopressin and ouabain to the corial bathing solution. results were compared with the amount of net sodium transport measured by short circuit current (scc). ouabain reduces scc ...19751081203
[proceedings: measurement of the secretory activity of the interrenal glands in rana esculenta l (author's transl)].in the green frog, the total corticosterone concentration measured by radiocompetitive binding assay, is 4,00 +/- 0,5 mug/ml. in captivity, this level is stable; in the natural environment, it changes with the hour and the date of the sampling. the percentage of bound corticosterone is about 30%.20051081362
colour discrimination functions of the frog optic tectum (rana esculenta). 19751081781
an analysis of muscle weight variations in the hindlimbs of rana esculenta.dry weights of 24 muscles of the left and of the right hindlimbs in 63 frogs (rana esculenta) are determined. by means of factor analysis is calculated which part of the variance in a muscle's weight can be accounted for by common factors and which part by the characteristics of the muscle itself. the main results are: 1. body size (factor 1) accounts for more than 90% of the variance in all the heavier muscles and more than 64% in the eight lighter muscles. 2. since only one single common facto ...19751082278
[seasonal changes in cutaneous blood vessel tone in the frog rana esculenta].experiments have been carried out on frogs immobilized by diplacine. the diameter of arteries and veins in the web was measured before and after sectioning the sciatic nerve. denervation of the hindlimb resulted in dilatation of web vessels, which was different at various seasons. changes in the arterial tone were found to be polyphasic. the highest tone was observed in march, april and september, the lowest one-in may, august, october and january. changes in the venous tone are less pronounced ...20081082690
proceedings: the brain stem of the axolotl ambystoma mexicanum and the frog rana esculenta. 19751082712
[nature of urea transport through the baso-lateral membrane of the urinary bladder of rana esculenta]. 19751082765
effect of conditioning potential on potassium current kinetics in the frog node.the kinetics of potassium conductance changes were determined in the voltage clamped frog node (rana esculenta), as a function of conditioning prepotential. the conditioning potential duration varied from 1 to 50 ms and the amplitude between -60 and +130 mv (relative to rest). the conductance kinetics were determined at a single test potential of +20 mv (depolarization) by means of the slope of log [ninfinity - nt] vs. time relationship which defines the time constant of the process (tau). the v ...19761082776
genetic control of ldh isozymes in the rana esculenta complex.studies of ldh isozymes in the european green frogs showed that the synthesis of the b subunits is controlled by 3 alleles at a single genetic locus. the genetic evidence supports the hypothesis that rana esculenta is the hybrid of r. lessonae x r. ridibunda.20051082820
synthesis and intracellular transport of proteins in the exocrine pancreas of the frog (rana esculenta). ii. an in vitro study of the transport process and the influence of temperature.frog pancreatic tissue was pulse-labelled in vitro with 3h-leucine and protein transport was studied in exocrine cells by electron microscope autoradiography. the proteins appeared to be synthesized in the rer and transported to the secretory granules along a similar route and with the same velocity as previously described under in vitro conditions. evidence was obtained for the involvement of the vesicular and tubular elements at the periphery of the golgi system in transferring protein from th ...19761083293
aluminium localization in amphibian skin with the electron microprobe and ion microanalyzer.emp and ima studies show the presence of a1 in the epidermis of larvae and young adults of discoglossus pictus and rana esculenta, which disappears during later stages of development. a1 is practically absent in the epidermis of xenopus laevis and pleurodeles waltlii. the localization of a1 with emp and ima supports the biochemical results. the function of a1 in the epidermis is not known at present.19761083571
topological analysis of the brain stem of the frogs rana esculenta and rana catesbeiana.the ventricular sulcal pattern and the cytoarchitectonic organization of the brain stem of the frogs rana esculenta and rana catesbeiana have been studied in transversely cut, nissl stained serial sections. four longitudinal sulci, the sulcus medianus inferior, the sulcus intermedius ventralis, the sulcus limitans and the sulcus medianus superior could be distinguished in both species. a fifth longitudinal groove, the sulcus intermedius dorsalis, was found only in rana esculenta. with the aid of ...19761083857
the implication of ribonucleic acids in oviduct growth in the green frog, rana esculenta. 19761084312
[observations on the ultrastructure of the chromaffin cells of rana esculenta during embryonal development and metamorphosis]. 20151084548
ciliated glial cells and neurons in the interpeduncular nucleus of the frog.some neurons and glial cells of the interpeduncular nucleus of the adult frog rana esculenta contain one cilium. the cilium complex shows two-centriole basal organization. cross striated structures are seen apposed to the centriolar formations. the centriole and associated basal body are always close to the vesicular elements of the golgi apparatus in the neurons and are surrounded by quite a number of microtubules in the glial cells. the observations suggest that these ciliated cells represent ...19751084631
[characterization and study of the origin of melanins isolated from the liver and skin of rana esculenta l]. 19751084755
[effect of histamine on the transport of non-electrolytes across the gastric mucosa of rana esculenta]. 19761084756
[structure and organization of the contractile proteins at the level of the z and m bands in the sartorius muscles of rana esculenta treated with methylxanthine (mx)]. 19761084757
[progressive muscular dystrophies in humans: micrographic aspects compared with striated muscles of rana esculenta treated with methylxanthine (mx)]. 19761084758
[preliminary studies on the presence of endogenous rhythm in the oviduct of rana esculenta]. 19751085154
[changes in the activity of adrenal glands in rana esculenta during the annual cycle: metabolism of corticosterone-4-14 c in the adrenal glands and determination of aldosterone and cortisol in the plasma]. 19751085155
[the erythropoietic activity of the spleen of rana esculenta, in the adult and during metamorphosis]. 19751085158
two populations of afferent fibres in the saccular nerve of the frog (rana esculenta). 19761085191
elemental distribution of na, p, cl and k in different structures of myelinated nerve of rana esculenta.measurements of the distribution of na, p, cl and k were performed in different structures of the myelinated nerve. whereas the axon shows a typical intracellular distribution pattern for na, cl and k, the interstitial space and the myelin sheath show a typical extracellular pattern. these measurements have demonstrated that na is present in the myelin sheath close to the node of ranvier.19761085262
some observations on the hormonal control of melanophores in the skin of the green frog rana esculenta l. 19761085286
asymmetrical displacement current and its relation with the activation of sodium current in the membrane of frog myelinated nerve.1. sodium currents (ina) and asymmetrical displacement currents (id) were measured in the same nerve fibres from rana esculenta under similar conditions. 2. for exploring possible kinetic and steady state relations between ina and id the following quantities were compared: (i) the activation of the sodium channels and (ii) the charge displacement of id. 3. the delay of sodium activation increased after hyperpolarization. a corresponding effect on the displacement of charge was not observed. 4. u ...19761085437
saxitoxin and procaine act independently on separate sites of the sodium channel.1. voltage clamp experiments were done on single myelinated nerve fibres of the frog, rana esculenta. 2. the time course of procaine action (1.0 mm at ph 7.2) was obtained from changes in ina on changing solutions during repetitive (1 hz) depolarizing pulses of constant amplitude following hyperpolarizing prepulses. the mean half times of onset and offset of procaine block were 3.7 and 28 s, respectively. in the presence of 1.4 nm saxitoxin (stx) the corresponding times were virtually the same, ...19761085438
a schematic dioptric apparatus for the frog's eye (rana esculenta). 19761085524
mitochondria-rich cells of frog skin in transport mechanisms: morphological and kinetic studies on transepithelial excretion of methylene blue.the following study combines histological observations on the localization and density of mitochondria-rich cells with a kinetic study of the part played by these cells in transport phenomena. in the ventral skin, mitochondria-rich cells and granular cells are equally abundant in the first living cell layer. the mitochondria-rich cells were shown to excrete methylene blue [3,9-bis-(dimethylamino)-phenozathionum chloride]. a kinetic study of this excretion across the isolated epithelium of rana e ...19761085569
effect of testosterone upon the beta-glucuronidase activity of some target tissues of the frog, rana esculenta. 19761085808
[synthesis of mucin in the oviduct of rana esculenta and lacerta sicula during the reproductive cycle]. 19751085983
the quality of the optic system of the frog's eye (rana esculenta). 19761086025
[study of the stilling cells of the adrenal of rana esculata after injection of triap (author's transl)]. 20061086077
[restoration of the behavioral reactions of rana esculenta frogs during regeneration of visual nerve fibers].after sectioning the optic nerve, various forms of visually guided behaviour in the frog r. esculenta recover unevenly. within 16--20 days, the onset of reactions was observed which are associated with perception of relatively large unmoving objects during active locomotion of animals (1); within 55 days -- avoidance reactions (2); within 90 days -- feeling reactions (3) were formed. in electrophysiological experiments it was shown that axons of the ganglionic retinal cells from various classes ...20061086565
[l-carnitine as a basis of cholinomimetic substances].acetylcarnitine, though having the same configuration as acetylcholine and acetyl-beta-methylcholine, is devoid of cholinomimetic properties as long as the carboxylic group is free. contrary findings are explainable by the lack of uniformity of the test substance, caused by substitution of the carboxylic group and intramolecular cleavage of water or acetic acid from carnitine or acetylcarnitine and by admixtures of active substances, and are attributable to the formation of metabolites in vivo. ...19761086568
decreased rate of sodium conductance inactivation in the node of ranvier induced by a polypeptide toxin from sea anemone.the effects of two toxins extracted from the tentacles of anemonia sulcata on ionic currents have been tested on the nodal membrane of myelinated nerve fibres from rana esculenta. while external application of toxin i at 100 mum leaves both specific ionic currents unmodified, toxin ii at 10 mum reacts with a receptor site associated with the sodium conductance inactivation gating. since internal application by diffusion of toxin ii at a concentration of 700 mum leaves the ionic currents unchange ...19761086682
tonic influence of the efferent vestibular system on the spontaneous afferent activity from semicircular canals in the frog (rana esculenta l.).in the frog we have recorded the spontaneous activity of single afferent fibres of the ampullary nerves of the left horizontal semicircular canal (hc) and vertical anterior canal (vac) in isolated head preparations. the recordings have been made in 4 experimental situations: intact preparations; preparations whose brain was destroyed; preparations whose contralateral vestibular nerve had been cut between scarpa's ganglion and the brain; preparations where either an ampullary nerve (that of the h ...19761086793
responses to tilting of fibers of the frog's saccular nerve.the activity of single fibers from the saccular nerve has been recorded in isolated head preparations in the frog (rana esculenta). nearly all the fibers present an activation or a depression of their activity during the tilting movement in a given direction. this is considered to correspond to the existence of two anatomical populations of haircells in the saccular macula which differ in their functional polarization. the activity of only some units (about 40%) remained increased or decreased w ...19761087003
block of potassium channels of the nodal membrane by 4-aminopyridine and its partial removal on depolarization.1. voltage clamp experiments were done on single myelinated nerve fibres of the frog, rana esculenta. 2. 53 mum 4-aminopyridine (4-ap) reduced ik to about one-fifth if tested with infrequent (1/min) and short (10 ms) depolarizing pulses; the onset time constant under these circumstances was ca. 160 s (14-15 degrees c). after prolonged treatment the effect was virtually irreversible. 3. at equilibrium with 4-ap, increasing the frequency of short pulses removed part of the block, the block removal ...20051087404
measurement of the conductance of the sodium channel from current fluctuations at the node of ranvier.single myelinated nerve fibres of rana esculenta were investigated under voltage clamp conditions at 13 degrees c. fluctuations of steady-state membrane current were measured during the last 152 msec of 190-225 msec pulses depolarizing the membrane by 8-48 mv. noise power spectral densities were calculated in the frequency range of 6-6-6757 hz. 2. external application of 150 nm tetrodotoxin (ttx) and/or 10 mm tetraethylammonium (tea) ion reduced the current fluctuations. the difference of curren ...20051087643
the vascular and bed capacity in the kidneys, spleen and liver of the edible frog (rana esculenta l.). 19761087895
[regeneration of the crystalline lens in anuran amphibia in the postembryonal stage. experimental excision of the crystalline lens in rana esculenta and xenopus laevis larvae]. 19761088056
[environmental factors and aldosterone secretion in the edible frog rana esculenta l (author's transl) (proceedings)]. 20161088341
[comparison of the growth rate of the hypophysis of a amphibian (rana esculenta) with that of a mammal (domestic albino mouse)]. 19761088463
morphological observations on the glands of the oesophagus and stomach of adult rana esculenta and bombina variegata.examination of serial sections of the oesophagus and stomach of rana esculenta and bombina variegata revealed that oesophageal peptic glands were only present in rana, where gastric glandular cells performed only one functional role, represented by hcl production. in contrast, in bombina the gastric glands were mainly of oxynticopeptic cells with dual oxyntic and peptic roles, reflected morphologically by distinct different areas of their cytoplasm. during activity of the oxynticopeptic cells cy ...20061285678
preparation and characterization of troponin c from bullfrog skeletal muscle.troponin c was isolated from the skeletal muscle of bullfrog (rana catesbeiana), and its relative molecular mass was estimated to be 18,000 by sds/polyacrylamide gel electrophoresis. in its amino acid composition, bullfrog troponin c was similar to that of the frog (rana esculenta) but different from that of rabbit. its ultraviolet spectrum was consistent with its amino acid composition. the ultraviolet difference spectrum of the ca(2+)-loaded form vs. the metal-free form indicated that the sing ...19921295889
the msh-like activity of pineal hormone e5 in vivo and in vitro experiment.further experiments have revealed that the polypeptidic hormone e5 has a msk-like effect, as experimentally demonstrated on the frog skin. in rana esculenta, 30-120 min after a subcutaneous injection of 0.2 mg purified e5b or e5p/100 g b.w., the skin blackened and the effect lasted for about two hours. a comparable effect was induced by incubating the fragment of frog abdominal skin with a similar solution of e5. this msk-like effect appears to be identical upon mammalian melanocytes in c57/b16 ...20151306087
the effect of the benzothiazepine diltiazem on force and ca2+ current in isolated frog skeletal muscle fibres.1. the action of the d-cis and l-cis isomers of the benzothiazepine derivative diltiazem on isometric force and l-type ca2+ inward current in short muscle fibres from toe muscles of the frog (rana esculenta and r. temporaria) was investigated under voltage clamp control with two internal microelectrodes. the experiments were performed at 10 degrees c in tea-sulphate solution, in which the concentration of ionized ca2+ was about 4 mm. 2. in the presence of diltiazem (d-cis, 0.25-30 microm; l-cis, ...19921323667
species differences in the stereoselectivity of kappa opioid binding sites for [3h]u-69593 and [3h]ethylketocyclazocine.stereoselectivity of the binding sites for the specific kappa-opioid agonist [3h]u-69593, a benzeneacetamido based ligand was investigated in membrane suspension prepared from frog and rat brain, as well as guinea pig cerebellum, using the pure chiral forms of different unlabelled opiates. the ligand binding sites showed stereospecificity with at least three orders of magnitude differences in the affinities (measured as ki values) of the opioid stereoisomer pairs both in rat and guinea pig membr ...19921331638
thermally induced chain exchange of frog alpha beta-tropomyosin.the thermally induced unfolding of the alpha-helix of rana esculenta alpha alpha, alpha beta and beta beta tropomyosin and two tryptic fragments approximately corresponding to the n- and c-terminal halves of alpha beta have been investigated by use of optical rotation, circular dichroism and uv difference spectroscopy. reversible unfolding transitions of alpha alpha and beta beta occur around 49 degrees c and 32 degrees c, respectively. the helix unfolding of alpha beta shows two major transitio ...19751334716
modulation of calcium current gating in frog skeletal muscle by conditioning depolarization.1. ca2+ inward currents were measured by voltage clamping cut skeletal muscle fibres of the frog (rana esculenta) in a double-vaseline-gap system. 2. in order to study the basis of the previously described fast gating mode induced in the ca2+ inward current by a conditioning depolarization we quantitatively analysed the response to differing features of the conditioning prepulse. 3. the faster activation seen during the second of two depolarizations was confined to the component of the inward cu ...19921338468
mitochondrial dna reveals formation of nonhybrid frogs by natural matings between hemiclonal hybrids.the european water frog rana esculenta (rl), a natural hybrid between r. ridibunda (rr) and r. lessonae (ll), reproduces by hybridogenesis: haploid gametes usually contain an intact chromosome set of r. ridibunda (r); the lessonae nuclear genome (l) is lost from the germ line. hybridity is restored in the next generation, via fertilization by syntopic r. lessonae. matings between two hybrids (rl x rl) usually give inviable r. ridibunda (rr) progeny. the adult r. ridibunda subpopulation of trubes ...19921352842
sodium nitroprusside alters dark voltage and light responses in isolated retinal rods during whole-cell recording.dark voltage and light responses of isolated retinal rods of rana esculenta were investigated by employing the whole-cell patch-clamp technique. when the recording pipette was filled with a medium devoid of nucleotides, a spontaneous hyperpolarization of the dark voltage partly due to a diffusional loss of cgmp and its precursor gtp and a retardation in the recovery of the light responses was observed. the larger part of the retardation of the light responses was prevented by 1 mm atp. addition ...19921354484
in vivo and in vitro effects of beta-endorphin and naloxone on corticosterone and cortisol release in male and female water frog, rana esculenta.1. beta-endorphin and naloxone effects on corticosterone and cortisol production in male and female rana esculenta, were studied in vivo and in vitro. 2. the in vivo and in vitro results were in agreement. 3. beta-endorphin caused a decrease in corticosterone and cortisol release. 4. naloxone induced an increase in the two corticosteroids at the same times as the decrease caused by beta-endorphin. 5. beta-endorphin plus naloxone treatment did not change corticosterone and cortisol levels. 6. the ...20061360359
the nature of cl- secretion, induced by carbaryl, across the isolated skin of rana esculenta.1. the pesticide carbaryl induces cl- secretion through the isolated frog skin. 2. this effect is due to the activation of both processes responsible for this phenomenon: (a) na+/k+/2cl- cotransport on the serosal membrane; (b) cl- selective channels on the external membrane. 3. cl- outflux is inhibited by bumetanide (10(-5) m) on the serosal side and by diphenylamine-2-carboxylic acid (dpc) (10(-3) m) on the external side. 4. the dpc action is not mimicked by naproxen, a specific inhibitor of c ...19921360392
some morphological and histochemical features of the midgut myenteric plexus of the common european frog, rana esculenta.the neuron morphology and distribution of four putative transmitters were investigated in the myenteric plexus of frog (rana esculenta) midgut. the gross morphology was revealed by nadh-diaphorase histochemistry, and the shape of the neurons by silver impregnation. nerve cells had heterogeneous distribution: they either formed ganglia or placed as solitary neurons in the duodenum, while in the rest of the midgut only solitary neurons were observed. three morphologically distinct cell types were ...19921374278
effects of intracellular signals on na+/k(+)-atpase pump activity in the frog skin epithelium.the effects of intracellular signals (phi, na+i, ca2+i, and the electrical membrane potential), on na+ transport mediated by the na+/k+ pump were investigated in the isolated rana esculenta frog skin. in particular we focussed on phi sensitivity since protons act as an intrinsic regulator of transepithelial na+ transport (jna) by a simultaneous control of the apical membrane na+ conductance (gna) and the basolateral membrane k+ conductance (gk). phi changes which modify jna, gna and gk, do not a ...19921374642
mallory stain may indicate differential rates of rna synthesis: i. a seasonal cycle in the harderian gland of the green frog (rana esculenta).when mallory's trichrome stain is used, acinar nuclei of the harderian gland of rana esculenta display different affinities for the dye. some of the orangiophilic nuclei show affinity for aniline blue (blue nuclei). in the harderian gland of rana esculenta their number and the intensity of staining with aniline blue may vary during the year. the affinity for aniline blue disappears following digestion of paraffin sections with rnaase, but not with dnaase or trypsin. furthermore, in vitro incubat ...19921374668
testosterone induction of poly(a)(+)-rna synthesis and [35s]methionine incorporation into proteins of rana esculenta harderian gland.the role of androgens in the cyclic secretory activity of the rana esculenta harderian gland (hg) was studied. total rna showed a dramatic increase in october and may when the nuclear androgen receptors peak. during the resumption of the secretory activity a gradual increase of poly(a)(+)-rna was detected; during the enhancement phase (may) a peak of the poly(a)(+)-rna fraction was found. in in vitro experiments testosterone increased the incorporation of [3h]uridine into the poly(a)(+)-rna frac ...19921375172
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