Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| eel (anguilla japonica) testis 11beta-hydroxylase gene is expressed in interrenal tissue and its product lacks aldosterone synthesizing activity. | a recombinant expression vector containing japanese eel (anguilla japonica) testis cytochrome p450(11 beta) (11beta-hydroxylase) cdna was introduced into cos-1 cells. enzymatic activity of the expressed p450(11 beta) for corticosteroid synthesis was analysed by incubating transfected cells with 14c-labelled 11-deoxycorticosterone or 3h-labelled deoxycortisol as substrates. thin layer chromatography of incubation medium revealed that a high percentage of 11-deoxycorticosterone was converted into ... | 1998 | 10022778 |
| natural mass infection by heterophyid metacercariae in aquacultured japanese eel in taiwan. | a natural mass infection of heterophyid metacercariae in aquacultured japanese eel anguilla japonica in taiwan was observed. of the 28,000 adult eels in 2 ponds, about 25,000 (90%) showed swollen, cloudy and white eyes. although morbidity was about 90%, there was no mortality among the affected eels. histopathological sections showed edema and hemorrhage of the eye. numerous metacercariae were observed in the muscle tissues around the eyeball, the subcutaneous tissue and even in the cartilage. o ... | 1999 | 10073313 |
| duality of gonadotropin in a primitive teleost, japanese eel (anguilla japonica). | the duality of gonadotropin (gth) is well-established in relatively modern teleosts. in primitive teleosts such as eel and catfish, however, only a single gth (gth-ii) has been isolated and characterized. therefore, the objective of this study was to clarify the duality of gth, particularly the presence of gth-i in primitive teleosts. we attempted to obtain a cdna encoding the beta subunit of gth-i from japanese eel, anguilla japonica, as a representative primitive teleost species. rapid amplifi ... | 1999 | 10094865 |
| development and differentiation of gonadotropin hormone-releasing hormone neuronal systems and testes in the japanese eel (anguilla japonica). | in this study we investigated the relationship between the development of the olfactory, preoptic, and midbrain gonadotropin-releasing hormone (gnrh) neuronal systems and testicular differentiation in eels (anguilla japonica) from embryonic stages through adulthood (5.4-50 cm body length). gnrh-synthesizing neuronal populations were first observed in the youngest fish ( approximately 5.0 cm) at the rostrobasal and caudalmost olfactory bulbs immunoreactive to a "promiscuous" (nonspecific) gnrh an ... | 1999 | 10336833 |
| susceptibility to oxidation of copper-induced plasma lipoproteins from japanese eel: protective effect of vitellogenin on the oxidation of very low density lipoprotein. | the susceptibility to oxidation of copper-induced plasma lipoproteins from japanese eel anguilla japonica was examined with the guidance of thiobarbituric acid-reactive substances (tbars). the tbars values of copper-induced plasma lipoproteins increased with increasing the lipid-to-apolipoprotein ratios and very low density lipoprotein (vldl) exhibited the highest tbars value. on the other hand, vitellogenin, estrogen-induced precursor of egg yolk proteins, was resistant to copper-induced oxidat ... | 1999 | 10390050 |
| gill lamellar pillar cell necrosis, a new birnavirus disease in japanese eels. | since the late 1980s, a birnaviral gill disease has been occurring in japanese eels anguilla japonica reared in warmwater ponds in western regions in japan. diseased eels mostly displayed marked formations of aneurysmal hematomas within gill lamellae and high mortalities. histological examination revealed necrosis of pillar cells and subsequent aggregation of erythrocytes inside the lamellar capillaries, and proliferation of interlamellar epithelia onto the lamellae. gastric gland cells were als ... | 1999 | 10439899 |
| chloride cell subtypes in the gill epithelium of japanese eel anguilla japonica. | the purpose of the present study was to characterize chloride cell subtypes in the fish gill and to monitor the kinetic change of cell division in the gill epithelia during seawater adaptation. employing a three-step percoll gradient method, the gill chloride cells and nonchloride cell population were isolated. the isolated cells were studied using multiparameter flow cytometry, recording the changes in 1) cell size, 2) cellular granularity, and 3) cell autofluorescence. two chloride cell subtyp ... | 1999 | 10444559 |
| cdna cloning of a stage-specific gene expressed during hcg-induced spermatogenesis in the japanese eel. | a single injection of human chorionic gonadotropin (hcg) can induce complete spermatogenesis in immature japanese eel (anguilla japonica) testes consisting of only premitotic spermatogonia. proliferation of spermatogonia, meiosis and spermiogenesis begin on 3, 12 and 18 days after hcg injection, respectively. to isolate the genes responsible for regulating the initiation of meiosis, differential mrna display using poly (a)+ rna extracted from testes of eels at different times after hcg treatment ... | 1999 | 10466934 |
| induction of two forms of eel cytochrome p450 1a genes by 3-methylcholanthrene. | : in eel (anguilla japonica), exposure to polyaromatic hydrocarbons such as 3-methylcholanthrene leads to induction of two cyp1a enzymes, cyp1a1 and cyp1a6. we studied the time course and tissue specificity of induction of messenger rnas for cyp1a1 and cyp1a6 in eel by administering 3-methylcholanthrene intraperitoneally. in both cases, the drug induced a rapid increase of mrnas and biphasic expression. in the liver, mrna levels of cyp1a1 and cyp1a6 increased 22-fold at 3 hours and 27-fold at 6 ... | 1999 | 10489409 |
| cloning and sequencing of cytochrome p450 1a complementary dna in eel (anguilla japonica). | : cytochrome p450 1a (cyp1a) complementary dna was isolated from eel (anguilla japonica) liver treated with 3-methylcholanthrene. the cdna contained a 5' untranslated region of 163 bp, an open reading flame of 1560 bp coding for 519 amino acids and a stop codon, and a 3' untranslated region of 1730 bp. the predicted molecular weight was approximately 58.4 kda. the deduced amino acid sequence exhibited identities with reported cyp1a sequences of 80% for rainbow trout, 79% for scup, 76% for plaice ... | 1999 | 10489411 |
| functional analysis of promoter region from eel cytochrome p450 1a1 gene in transgenic medaka. | : transcription of the cyp1a1 genes in mammals and fish is stimulated by polyaromatic hydrocarbons. dna sequencing analysis revealed that cyp1a1 gene in eel (anguilla japonica) contains two kinds of putative cis-acting regulatory elements, xre (xenobiotic-responsive element) and ere (estrogen-responsive element). xre is known as the enhancer that is responsible for the inducibility of the genes of cyp1a1 and some other drug-metabolizing enzymes. in the eel cyp1a1 gene, xre motifs are distributed ... | 1999 | 10489413 |
| isolation and sequence analysis of the eel cytochrome p450 1a1 gene. | : the putative cyp1a1 gene was isolated from a genomic library of eel (anguilla japonica) chromosomal dna. sequence analysis of 8184 bp showed that the clone obtained was the structural gene of cyp1a1, which consisted of seven exons and six introns in a region approximately 5800 bp in length, as well as a 5' upstream region of about 2300 bp. the initiation codon was found not in the first exon, but in the second exon, as was reported for the cyp1a genes of mammals and fish. the gc box, caat box, ... | 1999 | 10489414 |
| recombinant human insulin-like growth factor i stimulates all stages of 11-ketotestosterone-induced spermatogenesis in the japanese eel, anguilla japonica, in vitro. | in this study, we examined the in vitro effects of insulin-like growth factor i (igf-i) in the presence or absence of 11-ketotestosterone (11-kt: the spermatogenesis-inducing hormone) on the proliferation of japanese eel (anguilla japonica) testicular germ cells. initially, a short-term culture (15 days) of testicular tissue with only type a and early type b spermatogonia (preproliferated spermatogonia) was carried out in leibovitz-15 growth medium supplemented with different concentrations of r ... | 1999 | 10491628 |
| random amplified polymorphic dna analysis of eel genome. | eel family is a huge one, in which many kinds of eels especially some migratory eels, bear strong resemblance to each other, and are therefore difficult to be identified. in this study 29 random primers were used to make rapd analysis for japanese eel (anguilla japonica), european eel (anguilla anguilla) and pike eel (muraenesox cinereus). and totally 299 fragments were counted. shared or specific fragments were counted and genetic similarity or genetic distance were calculated. the genetic simi ... | 1999 | 10520604 |
| an enhanced humoral immune response against the swimbladder nematode, anguillicola crassus, in the japanese eel, anguilla japonica, compared with the european eel, a. anguilla. | the humoral immune response in the two eel species, anguilla japonica and anguilla anguilla against two fractions of antigens in anguillicola crassus were studied. within species, both eel species showed significantly elevated titres compared with controls when immunized with antigens from anguillicola crassus. in interspecific comparison, anguilla japonica showed significantly elevated titres in comparison with anguilla anguilla. immunization of anguilla anguilla caused a significantly decrease ... | 1999 | 10526415 |
| estradiol-17beta stimulates the renewal of spermatogonial stem cells in males. | in this work, we examined the functions of the female hormone "estrogen" on spermatogenesis of the japanese eel (anguilla japonica). estradiol-17beta (e(2)), a natural estrogen in vertebrates, was present in the serum and its receptor was expressed in the testis during the whole process of spermatogenesis. spermatogonial stem cell renewal was promoted by e(2) implantation but was suppressed by tamoxifen (an antagonist of estrogen). in vitro, 10 pg/ml of e(2) was sufficient to induce spermatogoni ... | 1999 | 10527870 |
| purification, characterization, and bioassay of prolactin and growth hormone from temperate basses, genus morone. | prolactin (prl) and two variants of growth hormone (gh), purified from pituitaries of striped bass (morone saxatilis) and its hybrid with white bass (m. saxatilis x m. chrysops) by gel filtration chromatography under alkaline conditions followed by reversed-phase high pressure liquid chromatography, appear similar between species. both the minor (gh i) and the major (gh ii) forms of purified gh appeared as single bands (m(r) approximately 23,000) after sodium dodecyl sulfate-polyacrylamide gel e ... | 2000 | 10620430 |
| characterization of a testicular 17alpha, 20beta-dihydroxy-4-pregnen-3-one (a spermiation-inducing steroid in fish) receptor from a teleost, japanese eel (anguilla japonica). | a cdna encoding a nuclear 17alpha,20beta-dihydroxy-4-pregnen-3-one (17alpha,20beta-dp, spermiation-inducing hormone in fish) receptor (dpr) was, for the first time, isolated from an eel testis cdna library. the amino acid sequence of dpr shows high homology with those of human and chicken progesterone receptors. the affinity of the bacterial recombinant dpr ligand binding domain protein for 17alpha,20beta-dp is higher than that of progesterone. in transfection experiments using cos7 cells, the d ... | 2000 | 10620698 |
| molecular cloning of fresh water and deep-sea rod opsin genes from japanese eel anguilla japonica and expressional analyses during sexual maturation. | we have determined the complete cdna sequences of fresh water rod opsin gene (fwo) and deep-sea rod opsin gene (dso) from japanese eel anguilla japonica. the cdna clones of fwo and dso consisted of 1437 and 1497 nucleotides, respectively. the predicted opsins of both genes consisted of 352 amino acid residues. southern blot and pcr analyses of genomic dna indicated that the japanese eel genome contains only one fwo and one dso and they are intronless. quantitative rt-pcr analyses revealed that t ... | 2000 | 10708752 |
| molecular cloning and characterization of japanese eel ovarian p450c17 (cyp17) cdna. | as a first step in investigating the mechanism underlying the steroidogenic shift from the production of ovarian androgens (vitellogenic stage) to that of 17alpha-hydroxylated progestins (maturational stage) in japanese eel during induced oogenesis, a cdna encoding japanese eel (anguilla japonica) ovarian p450c17 (cyp17: steroid 17alpha-hydroxylase/c(17-20) lyase) was cloned and sequenced. this cdna contained the complete coding region representing 510 amino acid residues, which showed high sequ ... | 2000 | 10753574 |
| pathological changes caused by cold-water stress in japanese eel anguilla japonica. | in the present study, histopathological changes in japanese eel anguilla japonica subjected to 'cold-water stress' were examined. eels were exposed to cold water (13 to 15 degrees c) and warmer water (25 degrees c) as controls. fish held in warm water did not show any apparent changes. although none of the eels exposed to cold water died, they displayed nephrotic changes such as cloudy swelling and hyaline droplet degeneration of the renal tubular epithelia. fish with nephroses had low levels of ... | 2000 | 10785862 |
| an eel cytochrome p450 1a gene having no xre sequences in its 5' upstream region of 1600 bp. | the gene family cytochrome p450 (cyp) 1a is ubiquitous among animals, and cyp1a1 genes have been identified in teleost and mammals. we isolated another cyp1a gene, which is inducibly expressed by exposure to 3-methylcholanthrene, from the genomic library of the eel (anguilla japonica). the genomic clone obtained, approximately 17,500 bp in length, contained the structural gene of the cyp1a and a 5' upstream region of about 1600 bp. sequence analysis of the 5' upstream region and the first and se ... | 2000 | 10811956 |
| monoclonal antibodies to plasma high density lipoprotein (hdl) of eel (anguilla japonica). | six week-old female mice (balb/c) injected intraperitonealy with 50 micrograms of eel high density lipoprotein (hdl) emulsified with equal volume of adjuvant three times every two weeks. three weeks after the third injection, hyperimmunized mice were boosted by injection of 100 micrograms of hdl. after 5 days, the best responding mouse to injected hdl was sacrificed, and spleen cells were fused with mouse myeloma cells (sp2/o-ag14), and hybridomas were cultured in a selection medium. monoclonal ... | 2000 | 10904860 |
| role of the renin-angiotensin system in drinking of seawater-adapted eels anguilla japonica: a reevaluation. | the role of ang ii, a potent dipsogenic hormone, in copious drinking of seawater eels was examined. sq-14225 (sq), an angiotensin-converting enzyme inhibitor, infused intra-arterially at 0.01-1 microgram. kg(-1). min(-1), depressed drinking and arterial blood pressure in a dose-dependent manner. the inhibition was accompanied by a small decrease in plasma ang ii concentration, which became significant at 1 microgram. kg(-1). min(-1). after the infusate was changed back to the vehicle, the depres ... | 2000 | 10956272 |
| effects of cortisol on chloride cells in the gill epithelium of japanese eel, anguilla japonica. | the purpose of the present study was to determine the effects of cortisol on the development of the freshwater chloride cell (cc), using flow cytometry. scanning electron microscopy was used to determine the corresponding modifications in cc apical structure. simultaneously, biochemical analyses were conducted to determine the activities of transport atpases, mitochondrial enzymes (succinate dehydrogenase (sdh) and mg(2+)-atpase) and lactate dehydrogenase (ldh). the effects of daily i.m. injecti ... | 2001 | 11139782 |
| immunocytochemical localization of taurine in the fish retina under light and dark adaptations. | previously we have observed the lack of immunoreactivity of taurine in the rod outer segments from light-adapted fish, such as the ayu plecoglossus altivelis and lefteye flounder paralichthys olivaceus. this finding prompted us to investigate if there is a difference in the immunocytochemical localization of taurine in the rod outer segments between the dark- and light-adapted states. in the retinas of the glass eel anguilla japonica and the young goldfish carassius auratus, extremely intense im ... | 2000 | 11140362 |
| ultrastructural justification for the transfer of pleistophora anguillarum hoshina, 1959 to the genus heterosporis schubert, 1969. | this study presents the ultrastructure of the microsporidian infecting the trunk musculature of anguilla japonica and originally described as pleistophora anguillarum hoshina, 1959. all stages develop within a special structure, the sporophorocyst (spc), which is equipped with a thick dense wall. this wall grows along with the growth of the parasites within it. meronts are uni- to binucleate, which divide and steadily give rise to sporonts. during transition to sporonts the cell coat of the mero ... | 2000 | 11206738 |
| the novel sequences of major plasma apolipoproteins in the eel anguilla japonica. | cdnas encoding major plasma apolipoproteins (apo) were cloned from the eel anguilla japonica liver and their nucleotide sequences determined. sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that eel lipoproteins contain apolipoproteins of 28 kda and 14 kda as major components. each of the two apolipoproteins showed two isoforms having different isoelectric points as demonstrated by two-dimensional electrophoresis. the two 28 kda components had different n-terminal amino acid s ... | 2001 | 11278178 |
| characterization of japanese eel immunoglobulin m and its level in serum. | japanese eel immunoglobulin m (igm) was purified from the sera of anguilla japonica immunized with edwardsiella tarda fpu 347 and characterized. analysis of the purified igm on sodium dodecyl sulfate-polyacrylamide gels (sds-page) under reducing and non-reducing conditions revealed that the eel igm was a tetrameric protein with a molecular weight of 790,000; it contained an equimolar heavy chain and light chain with molecular weights of 72,000 and 25,000, respectively. while the n-terminal seque ... | 2000 | 11281270 |
| genome sequence of a vp2/ns junction region of pillar cell necrosis virus (pcnv) in cultured japanese eel anguilla japonica. | pillar cell necrosis virus (pcnv) is an aquatic birnavirus that was isolated from farmed japanese eel experiencing mass mortality. in this study, a vp2/ns junction region in genome segment a of pcnv was amplified by reverse transcriptase polymerase chain reaction (rt-pcr) and sequenced. the vp2/ns region in pcnv had the highest homology with that of a strain ab of infectious pancreatic necrosis virus (ipnv). this result revealed that pcnv belongs to birnavirus genogroup ii. | 2001 | 11383565 |
| two subtypes of androgen and progestogen receptors in fish testes. | two subtypes (alpha and beta) of androgen (ar) and progestogen receptors (pr) are present in the testis of japanese eel (anguilla japonica). amino acid homology of the open reading frames between alpha and beta in ar or pr is approximately 40%, but the dna- and ligand-binding domains show high homology between subtypes. judging from these structures, alpha and beta are not isoforms derived from translational initiation at two in-phase atg codons, alternative splicing, or tetraploidy. in transien ... | 2001 | 11399479 |
| high density lipoprotein binding protein of eel (anguilla japonica) liver with specificity of binding to apoai as a ligand. | high-density lipoprotein (hdl) binding protein (hbp) was isolated from the microsomal fraction of eel liver homogenate by affinity chromatography with a hdl-column. after sds-page and blotting, hbp on the pvdf membrane was detected by fitc-labeled hdl and apolipoprotein ai (apoai) as a ligand. hbp in the microsomal fraction was most abundant among microsomal, mitochondrial and cytosolic fractions. the hbp isolated by a hdl-column consisted of at least three proteins with low molecular weights of ... | 2001 | 11435139 |
| biofilter treatment of aquaculture water for reuse applications. | biotreatment of aquaculture water for recirculation purposes is a sensible mean to support the further growth of aquaculture industry without excessive water demands that are environmentally unsustainable. this study evaluates the efficacy of biofilter treatment of an eel (anguilla japonica) culture pond water using different filter media and flow scheme arrangements. the experimental results demonstrate that biofilter systems packed with suitable filter media are capable of improving the qualit ... | 2001 | 11487106 |
| glucagon-like peptide isolated from the eel intestine: effects on atrial beating. | a new glucagon-like peptide was isolated from the intestine of the eel anguilla japonica. the primary structure was determined by sequence analysis after cleavage with lysyl endopeptidase, quantitative amino acid analysis and fast atom bombardment mass spectrometry as hsqgtftndy(10)skyletrraq(20)dfvqwlmnsk(30)rsggpt. since its structure is similar to that of oxyntomodulins (oxms) reported in various vertebrates, we named this peptide eel oxyntomodulin (eoxm). we found that eoxm enhanced the cont ... | 2001 | 11551990 |
| antidipsogenic effects of eel bradykinins in the eel anguilla japonica. | a peptide with bradykinin (bk)-like immunoreactivity was isolated from an incubate of heat-denatured eel plasma with porcine pancreatic kallikrein. the purified peptide had the following amino acid sequence: arg-arg-pro-pro-gly-ser-trp-pro-leu-arg. this decapeptide, named eel [arg(0)]bk, was identical to two previously identified bk homologs from cod and trout. high conservation of the bk sequence among distant teleost species suggests an important function in this vertebrate group. bolus intra- ... | 2001 | 11557614 |
| [studies on the crude drugs used for the folk medicine described in "mimi-bukuro"]. | "mimi-bukuro" is a book written by moriyasu negishi in the edo period. m. negishi (1737-1815) was a magistrate in the town of edo. he was very much interested in listening and recording many kinds of stories, which were told by the various kinds of people, such as public officers, samurais, merchants, doctors, etc. among the stories of this book, some stories were found concerning folk medicine, medicinal substance and charms. in this report, i studied the medicines which were used in these stor ... | 1995 | 11613523 |
| structure, properties and enhanced expression of galactose-binding c-type lectins in mucous cells of gills from freshwater japanese eels (anguilla japonica). | using a japanese-eel (anguilla japonica) gill cdna subtraction library, two novel beta-d-galactose-binding lectins were identified that belong to group vii of the animal c-type lectin family. the eel c-type lectins, termed ecl-1 and ecl-2, are simple lectins composed of 163 amino acid residues, including a 22-residue signal peptide for secretion and a single carbohydrate-recognition domain (crd) of approximately 130 residues typical of c-type lectins. the galactose specificity of the crd was sug ... | 2001 | 11695997 |
| a novel progestogen receptor subtype in the japanese eel, anguilla japonica. | a cdna encoding a second type of a progestogen receptor (epr2) was isolated from the same library as we had previously cloned a functional pr (epr1) in eel testis. the amino acid sequence of the epr2 shows low homology with epr1 (34%), but both prs showed progestogen-dependent transactivation in transfection experiment. tissue distribution of epr2 mrna was clearly different from that of epr1. protein interaction between two prs was demonstrated in vitro by a glutathione s-transferase pull-down a ... | 2002 | 11755535 |
| changes in hepatic vitellogenin mrna levels during oocyte development in the japanese eel, anguilla japonica. | the induction of vitellogenesis is a complex process requiring coordinated control and expression of many hepatic gene products, such as vitellogenin (vtg). to investigate the regulation of vtg synthesis, knowledge of the molecular genetics of vtg is required. here, the authors have isolated a partial cdna encoding japanese eel vtg using an immunoscreening technique. this cdna contained an open reading frame of 1629 bp, predicted to encode 543 amino acid residues, the sequence of which showed hi ... | 2002 | 11825029 |
| the natriuretic peptide system in eels: a key endocrine system for euryhalinity? | the natriuretic peptide system of a euryhaline teleost, the japanese eel (anguilla japonica), consists of three types of hormones [atrial natriuretic peptide (anp), ventricular natriuretic peptide (vnp), and c-type natriuretic peptide (cnp)] and four types of receptors [natriuretic peptide receptors (npr)-a, -b, -c, and -d]. although anp is recognized as a volume-regulating hormone that extrudes both na(+) and water in mammals, anp more specifically extrudes na(+) in eels. accumulating evidence ... | 2002 | 11893596 |
| primary structure and characteristics of a lectin from skin mucus of the japanese eel anguilla japonica. | two types of lactose-binding lectins, ajl-1 and ajl-2, were purified from the skin mucus extract of the japanese eel anguilla japonica by lactose affinity chromatography and subsequent gel filtration. the molecular masses of ajl-1 and ajl-2 were 16,091 and 31,743 da, respectively. intact ajl-1 was comprised of two identical 16-kda subunits having blocked n termini and no disulfide bonds. ajl-2 was a homodimer with disulfide bonds. based on the n-terminal amino acid sequence of the ajl-2 monomer, ... | 2002 | 11959866 |
| pcna protein expression during spermatogenesis of the japanese eel (anguilla japonica). | spermatogenesis can be initiated by a single injection of human chorionic gonadotropin (hcg) into the cultivated japanese eel, which produces only spermatogonia in the testis. to isolate the genes responsible for regulating spermatogenesis, we performed a differential mrna display using poly (a)+ rna extracted from the testes at different time points after hcg injection. among several cdna clones, the expression of which was initiated before the onset of meiosis, one clone has high homology with ... | 2002 | 12025409 |
| three mrna species for mammalian-type gonadotropin-releasing hormone in the brain of the eel anguilla japonica. | a variant mrna species for mammalian-type gonadotropin-releasing hormone (mgnrh) that retains first intron has recently been found in the brain of a primitive teleost, the eel anguilla japonica. here we have found that this mrna species is generated from an alternative upstream transcription start site of the mgnrh gene. in addition, the upstream start site produces another mrna species that lacks first intron. both of these two variant mrna species, however, have the same open reading frame as ... | 2002 | 12088863 |
| comparative studies between in vivo and in vitro spermatogenesis of japanese eel (anguilla japonica). | in order to check the quality of in vitro spermatogenesis of japanese eel, in vitrol 1-ketotestosterone (11-kt) induced spermatogenesis was compared with in vivo spermatogenesis induced by a single injection of human chorionic gonadotropin (hcg) in detail. dna contents of germ cells from in vitro and in vivo testicular fragments were compared using flow cytometry. since the in vitro result of flow cytometry showed prominent 1c peak including spermatozoa and spermatids, the reduction of dna by me ... | 2002 | 12125930 |
| development of lateral line organs in leptocephali of the freshwater eel anguilla japonica (teleostei, anguilliformes). | a study of the ontogeny of the lateral line system in leptocephali of the japanese eel anguilla japonica reveals the existence of three morphologically different types of lateral line organs. type i is a novel sensory organ with hair cells bearing a single kinocilium, lacking stereocilia, distributed mainly on the head of larvae, and morphologically different from typical superficial neuromasts of the lateral line system. its developmental sequence suggests that it may be a presumptive canal neu ... | 2002 | 12219345 |
| sequence and expression of androgen receptor and estrogen receptor gene in the sex types of protogynous wrasse, halichoeres trimaculatus. | sex steroid hormones play important roles in sex change and behavior of wrasses. their actions are considered to be mediated through the nuclear hormone receptors. in this study, to elucidate the roles of estrogen receptor (er) and androgen receptor (ar) in the reproduction of the protogynous wrasse, halichoeres trimaculatus, ar and er genes were partially cloned using 5'- and 3'-race and their transcript levels in the gonads and the brain were measured by competitive rt-pcr. the amino acid sequ ... | 2002 | 12383444 |
| pseudodactylogyrus kamegaii sp. n. (monogenea: pseudodactylogyridae) from wild japanese eel, anguilla japonica. | the monogenean pseudodactylogyrus kamegaii sp. n. is described, based on specimens collected from the gills of wild japanese eel anguilla japonica caught in chiba prefecture, japan. this species is the most similar to p. anguillae (yin and sproston, 1948), but different in the shape and measurements of the male copulatory organ, vagina and marginal hook. this new species was collected from the eel in brackish waters, while p. anguillae and p. bini, the other known pseudodactylogyrids of japanese ... | 2002 | 12421631 |
| ring finger, b-box, and coiled-coil (rbcc) protein expression in branchial epithelial cells of japanese eel, anguilla japonica. | an rbcc (ring finger, b-box, and coiled-coil) protein was identified that belongs to the superfamily of zinc-binding proteins and is specifically expressed in the gill of eel, anguilla japonica. euryhaline fishes such as eels can migrate between freshwater and seawater, which is considered to be accomplished by efficient remodeling of the architecture and function of the gill, a major osmoregulatory organ. to identify molecules involved in such adaptive changes, we performed differential display ... | 2002 | 12473111 |
| purification and characterization of dermatan sulfate from the skin of the eel, anguilla japonica. | glycosaminoglycans were isolated from the eel skin (anguilla japonica) by actinase and endonuclease digestions, followed by a beta-elimination reaction and deae-sephacel chromatography. dermatan sulfate was the major glycosaminoglycan in the eel skin with 88% of the total uronic acid. the content of the idoa2salpha1-->4galnac4s sequence in eel skin, which shows anticoagulant activity through binding to heparin cofactor ii, was two times higher than that of dermatan sulfate from porcine skin. the ... | 2003 | 12543559 |
| in vitro induction of luteinizing hormone synthesis by estrogen in organ-cultured pituitary glands of the japanese eel, anguilla japonica. | an organ culture method for pituitary glands isolated from immature japanese eels (anguilla japonica) was developed. this method could conserve the histological features of the pituitary glands for at least 21 days. the ability to synthesize gonadotropic hormone (gth) in cultured eel pituitary glands was examined by detecting luteinizing hormone (lh) beta protein immunohistochemically. in a basal medium (leibovitz l-15), lh beta-immunoreactive cells were very scarce, but after addition of estrad ... | 2003 | 12560603 |
| c-type natriuretic peptide of rainbow trout (oncorhynchus mykiss): primary structure and vasorelaxant activities. | natriuretic peptides (nps) play important roles in osmoregulatory and cardiovascular systems of vertebrates. for functional studies of nps, rainbow trout (oncorhynchus mykiss), a euryhaline fish, is an interesting model. the information on homologous nps of salmonid fish is, however, still incomplete with respect to c-type np (cnp). in this study, we isolated cdnas encoding the precursor of cnp from the brain of trout. predicted mature cnp (cnp-22) sequence was identical to that of killifish fun ... | 2003 | 12568796 |
| characterization of a cdna encoding p-450 aromatase (cyp19) from japanese eel ovary and its expression in ovarian follicles during induced ovarian development. | a cdna encoding p450 aromatase (cyp19) was isolated from a japanese eel (anguilla japonica) ovarian cdna library. this cdna contains a complete open reading frame encoding 511 amino acid residues. the deduced amino acid sequence is 59% and 65% identical to the catfish and rainbow trout forms, respectively, and 52-54% to mammalian and chicken forms. non-steroidogenic cos-7 cells transfected with the eel cyp19 cdna converted exogenous androstenedione to estrone, thus verifying its identity. northe ... | 2003 | 12568797 |
| ovarian mitochondrial cytochrome b mrna levels increase with sexual maturity in freshwater eels (anguilla spp.). | differential display of mrna was used to identify an upregulated gene in ovaries of artificially maturing japanese eels (anguilla japonica). accordingly, mitochondrial (mt) cytochrome b, whose transcript levels increased from early to late vitellogenesis, was isolated, cloned and sequenced. temporal trends in artificially maturing eels were compared with those in naturally and artificially maturing new zealand eels (longfinned eel, anguilla dieffenbachii; shortfinned eel, anguilla australis) by ... | 2003 | 12592438 |
| amidated fish ghrelin: purification, cdna cloning in the japanese eel and its biological activity. | we purified ghrelin from stomach extracts of a teleost fish, the japanese eel (anguilla japonica) and found that it contained an amide structure at the c-terminal end. two molecular forms of ghrelin with 21 amino acids were identified by cdna and mass spectrometric analyses: eel ghrelin-21, gss(o-n-octanoyl)flspsqrpqgkdkkpp rv-amide and eel ghrelin-21-c10, gss(o-n-decanoyl) flspsqrpqgkdkkpprv-amide. northern blot and rt-pcr analyses revealed high gene expression in the stomach. low levels of exp ... | 2003 | 12630926 |
| steroidogenic acute regulatory protein in eels: cdna cloning and effects of acth and seawater transfer on its mrna expression. | steroidogenic acute regulatory protein (star) is a key molecule for steroid production by translocating cholesterol from the outer to inner mitochondrial membrane. two cdnas of different length encoding star was cloned from the head kidney of the eel (anguilla japonica). in the 3'-untranslated region (utr) of the longer cdna, two putative polyadenylation signals were found. the shorter one differed from the longer one solely by the lack of middle of 3'-utr including the first polyadenylation sig ... | 2003 | 12655184 |
| flow cytometric analysis of the neutrophil respiratory burst of ayu, plecoglossus altivelis: comparison with other fresh water fish. | neutrophils of vertebrates undergo respiratory burst activity (rba) as a defense mechanism against bacterial infections. we report here that ayu (plecoglossus altivelis) have unusually high rbas even when they are in a healthy condition. kidney and blood leukocytes were obtained from ayu, rainbow trout (oncorhynchus mykiss), carp (cyprinus carpio), eel (anguilla japonica), and pond smelt (hypomesus nipponensis). neutrophil rba was measured by flow cytometry using dihydrorhodamine after stimulati ... | 2003 | 12787685 |
| profiles of pgh-alpha, gth i-beta, and gth ii-beta mrna transcript levels at different ovarian stages in the wild female japanese eel anguilla japonica. | the complete complementary dna (cdna) encoding pituitary gonadotropin ii-beta subunit (gth ii-beta) of japanese eel anguilla japonica was cloned and sequenced, and the profiles of pituitary glycoprotein hormone alpha subunit (pgh-alpha), gth i-beta, and gth ii-beta mrna transcript levels at different stages of ovarian development before vitellogenesis in the wild females were investigated. the maturity of female eels was divided into four stages: juvenile, sub-adult, pre-silver, and silver stage ... | 2003 | 12899842 |
| [the wormicidal substances of fresh water fishes on clonorchis sinensis: ii. preliminary research on the wormicidal substances from mucous substances of various fresh water fishes] | the present work which was investigated in july 1979, was to observe the wormicidal effects of the external mucous substances of 9 species of fresh water fishes (cyprinus carpio, parasilurus asotus, anguilla japonica, ophicephalus argus, carassius carassius; golden crusian carp, misgurnus anguillicaudatus, zacco platypus, pseudorasbora parva and carassius carassius; crusian carp) on cercaria, liberated metacercaria and adult of clonorchis sinensis. the mucous substances extracted by ether from t ... | 1980 | 12902746 |
| [experimental studies on the second intermediate hosts of clonorchis sinensis: iii. observations on the relationship between clavate cells of epidermis and infectivity of metacercariae of clonorchis sinensis in fresh-water fish] | in order to know relationship between the clavate cells of epidermis and infectivity of metacercariae of c. sinensis. histological investigation of epidermis was carried out on the 26 species of fresh-water fish collected from the main rivers in korea. results were summarized as it follows: 1. in general clavate cell had a single nucleus at its center. however it was observed in liobagrus mediadiposalis and pelteobagrus fuluidraco that clavate cell had two nuclei with and independent so-called l ... | 1974 | 12913471 |
| growth differences and growth hormone expression in male and female european eels [anguilla anguilla (l.)]. | in this study, we examined the growth differences of males and females following a sex reversion, and the growth hormone (gh) expression variation between sexes of european eels [anguilla anguilla (l.)]. a high percentage of females (88%) was found in the group fed with estradiol 17beta compared to the control group (comprised of only 6% female eels), which was defined as the male population. significant differences between growth rate and size were found following 480 days of growth, whereby th ... | 2003 | 13129507 |
| studies in neurosecretion. iii. nuclear secretion in the cells of the preoptic nucleus in the eel (anguilla japonica). | 1955 | 13241431 | |
| electrical activity of the caudal neurosecretory cells in the eel anguilla japonica with special reference to synaptic transmission. | 1962 | 13956803 | |
| hemoglobins from erythrocytes of eel, anguilla japonica. | 1964 | 14135455 | |
| studies in neurosecretion. i. preoptico-subcommissural neurosecretory system in the eel (anguilla japonica). | 1954 | 14380182 | |
| isolation, characterization and expression of 11beta-hydroxysteroid dehydrogenase type 2 cdnas from the testes of japanese eel (anguilla japonica) and nile tilapia (oreochromis niloticus). | the japanese eel (anguilla japonica) and nile tilapia (oreochromis niloticus) 11beta-hydroxysteroid dehydrogenase type 2 (11beta-hsd2) cdnas were isolated from their respective testes cdna libraries. the cdnas predict two peptides of 436 and 406 amino acid residues that share about 42% homology with mammalian 11beta-hsd type 2 proteins. analysis of the tissue distribution pattern by rt-pcr reveals that 11beta-hsd2 is expressed in a wide variety of tissues in tilapia, with higher expression in ki ... | 2003 | 14519098 |
| interrenal steroid 21-hydroxylase in eels: primary structure, progesterone-specific activity and enhanced expression by acth. | cytochrome p450 21-hydroxylase (p450c21) is a key enzyme for corticosteroidogenesis. to understand the regulatory mechanisms of cortisol production in fish, we have cloned a cdna encoding p450c21, for the first time in non-mammalian vertebrates, from the head kidney of the eel (anguilla japonica). the overall similarity of the deduced p450c21 sequence was modest (41-44% amino acid identity) between the eel and mammals. however, the functional domains for steroid-binding, heme-binding and proton- ... | 2003 | 14519100 |
| expression of a biologically active recombinant follicle stimulating hormone of japanese eel anguilla japonica using methylotropic yeast, pichia pastoris. | in the japanese eel anguilla japonica, the administration of exogenous gth is necessary for the artificial induction and completion of gonadal maturation due to its gth deficiency under captive conditions. the isolation of native eel gth has not been accomplished, which has made it difficult to fully elucidate the biological functioning of the two gths (fsh and lh) in eel. in this study, we attempted to produce a recombinant japanese eel gth (rjefsh) having biological activity using methylotroph ... | 2003 | 14636631 |
| molecular diversity of skin mucus lectins in fish. | among lectins in the skin mucus of fish, primary structures of four different types of lectin have been determined. congerin from the conger eel conger myriaster and ajl-1 from the japanese eel anguilla japonica were identified as galectin, characterized by its specific binding to beta-galactoside. eel has additionally a unique lectin, ajl-2, which has a highly conserved sequence of c-type lectins but displays ca(2+)-independent activity. this is rational because the lectin exerts its function o ... | 2003 | 14662297 |
| serum estradiol-17beta and testosterone levels during silvering in wild japanese eel anguilla japonica. | to understand the changes of serum levels of sex steroids in the wild japanese eel anguilla japonica during silvering process, eels collected from the kaoping river of taiwan from august 2000 through june 2001 were examined. the maturational stages of female eels before and during silvering were divided into four stages: juvenile, sub-adult, pre-silver and silver stages based on skin coloration and oocyte diameter. male eels were investigated only in the silver stage. radioimmunoassays were empl ... | 2003 | 14662313 |
| characteristics and primary structure of a galectin in the skin mucus of the japanese eel, anguilla japonica. | the characteristics and primary structure of ajl-1, one of the lectins in the skin mucus of the japanese eel (anguilla japonica), were examined. this lectin exhibited beta-galactoside specific activity in a ca2+ independent manner. we previously reported that its molecular mass was 16,091da, although it was approximately 30 kda as determined by gel filtration, indicating that it is a homodimer having non-covalent bonds. this lectin was composed of 142 amino acid residues having no half-cystinyl ... | 2004 | 14698218 |
| natriuretic peptides inhibit adenylyl cyclase activity in dispersed eel gill cells. | the effect of natriuretic peptides on forskolin-evoked adenylyl cyclase activity was investigated in dispersed gill cells from the australian short-finned eel (anguilla australis). molecular cloning techniques were employed to identify the putative g-protein-activating motif within the intracellular domain of the eel natriuretic peptide c receptor. eel anp, eel cnp and the npr-c-specific c-anf inhibited the forskolin-stimulated production of cyclic amp. this effect was abolished by pretreatment ... | 2004 | 14760501 |
| cloning of the cdna for thyroid stimulating hormone beta subunit and changes in activity of the pituitary-thyroid axis during silvering of the japanese eel, anguilla japonica. | the purposes of this study were: (1) to clone the cdna encoding pituitary thyroid-stimulating hormone beta subunit (tsh beta) of the japanese eel, anguilla japonica, together with its genomic dna sequence, for phylogenetic analysis, and to study the regulation of the tsh beta gene expression in cultured pituitaries; and (2) to investigate the transcript levels of pituitary tsh beta mrna and the serum thyroxine profiles at different stages of ovarian development before and during silvering in the ... | 2004 | 14766001 |
| anatomical studies on the labyrinth of anguilla japonica. | 1951 | 14884173 | |
| effect of various sorts of ion on the sensoria of the labyrinth of eel (anguilla japonica). | 1951 | 14884175 | |
| lesser known aquarium fish tumor models. | the repeated use of particular species for experimental oncology in fish increases their future value by accumulating background information for these models and justifies the establishment of genetic stock centers. however, the wide diversity that exists within the class osteichthyes and chondrichthyes suggests that the ideal surrogate models for studying some types of neoplasms might be found among lesser known species. to help assess cultured fish as surrogates for some other types of human n ... | 2001 | 14961307 |
| cloning, sequencing, and phylogenetic analysis of complementary dna of novel cytochrome p-450 cyp1a in japanese eel (anguilla japonica). | complementary dna of cytochrome p-450 cyp1a, in addition to cyp1a1, has been isolated from japanese eel (anguilla japonica) liver treated with 3-methylcholanthrene. the cdna contained a 5' untranslated region of 66 bp, an open reading frame of 1554 bp coding for 517 amino acids and a stop codon, and a 3' untranslated region of 1166 bp. the predicted molecular weight of the japanese eel cyp1a was approximately 58.5 kda. the nucleotide sequence exhibited identities with the reported cyp1a1 sequenc ... | 2001 | 14961358 |
| polymorphisms of ga/gt microsatellite loci from anguilla japonica. | six novel microsatellite loci, containing (ga)(15\n17) or (gt)(10\n19) perfect tandem repeats, were isolated and characterized for the catadromous eel anguilla japonica. the allelic size of the 6 loci ranged from 79 to 226 bp in length. all loci were polymorphic with a mean number of 14.7 alleles per locus and a mean heterozygosity of 0.67, suggesting higher polymorphism than that of freshwater and anadromous fishes, but lower than that of marine fishes. genotype diversity of the 6 loci ranged f ... | 2001 | 14961365 |
| structure and developmental expression of hatching enzyme genes of the japanese eel anguilla japonica: an aspect of the evolution of fish hatching enzyme gene. | we isolated seven cdna clones from embryos of the japanese eel anguilla japonica. each deduced amino acid sequence consisted of a signal peptide, a propeptide and a mature enzyme portion belonging to the astacin protease family. a phylogenetic analysis showed that the eel enzymes resembled the high choriolytic enzyme (hce) of medaka oryzias latipes, and the hatching enzymes of the zebra fish danio rerio and masu salmon oncorhynchus masou. hatching enzymes of these teleosts belonged to the group ... | 2004 | 15014992 |
| a single amino acid substitution can shift the optimum ph of dnase i for enzyme activity: biochemical and molecular analysis of the piscine dnase i family. | we purified four piscine deoxyribonucleases i (dnases i) from anguilla japonica, pagrus major, cryprus carpio and oreochromis mossambica. the purified enzymes had an optimum ph for activity of approximately 8.0, significantly higher than those of mammalian enzymes. cdnas encoding the first three of these piscine dnases i were cloned, and the sequence of the takifugu rubripes enzyme was obtained from a database search. nucleotide sequence analyses revealed relatively greater structural variations ... | 2004 | 15182937 |
| magnetic sense in the japanese eel, anguilla japonica, as determined by conditioning and electrocardiography. | magnetosensitivity of the japanese eel, anguilla japonica, was examined by conditioning and electrocardiography. marine eels, river eels and farmed eels were conditioned to an imposed magnetic field ranging from 12,663 nt to 192,473 nt parallel to the fish body, which was placed along the earth's west-east axis. electrocardiograms were recorded with electrodes placed close to the fish body inside a pvc pipe shelter. after 10-40 conditioning runs, all the eels exhibited a significant conditioned ... | 2004 | 15277551 |
| the cloning of cyclin b3 and its gene expression during hormonally induced spermatogenesis in the teleost, anguilla japonica. | we cloned cyclin b1, b2, and b3 cdnas from the eel testis. northern blot analysis indicated that these cyclin b mrnas were expressed and increased from day 3 onward after the hormonal induction of spermatogenesis, and that cyclin b3 was most dominantly expressed during spermatogenesis. in situ hybridization showed that cyclin b1 and b2 were present from the spermatogonium stage to the spermatocyte stage. on the other hand, cyclin b3 mrna was present only in spermatogonia. although mouse cyclin b ... | 2004 | 15351735 |
| applying dna techniques to the identification of the species of dressed toasted eel products. | to differentiate the species of processed eel products, the gene identification of four fresh eel species was first established and the species of eel products collected from markets were investigated. polymerase chain reaction (pcr) and sequence analysis were used to determine the genetic variation in a 362-nucleotide region of the mitochondrial cytochrome b gene in four fresh eels including anguilla japonica, anguilla anguilla, anguilla rostrata, and muraenesox cinereus. it was found that each ... | 2004 | 15366851 |
| differences in susceptibility of the european eel (anguilla anguilla) and the japanese eel (anguilla japonica) to the swim-bladder nematode anguillicola crassus. | the swim-bladder nematode anguillicola crassus originates from the far east where it is a parasite of the japanese eel (anguilla japonica). after a. crassus was introduced to europe, it became a predominant parasite of the european eel (anguilla anguilla). a study performed with experimentally infected eels (98 days, 23 degrees c) revealed significant differences in the susceptibility of the two eel species to this parasite. the recovery rate of 30 administered infective a. crassus larvae (l3) f ... | 2004 | 15521638 |
| isolation and characterization of active line and sines from the eel. | long interspersed elements (lines) and short interspersed elements (sines) are retrotransposons. these elements can mobilize by the "copy-and-paste" mechanism, in which their own rna is reverse-transcribed into complementary dna (cdna). lines and sines not only are components of eukaryotic genomes but also drivers of genomic evolution. thus, studies of the amplification mechanism of lines and sines are important for understanding eukaryotic genome evolution. here we report the characterization o ... | 2005 | 15548748 |
| effects of 4-tert-pentylphenol on the gene expression of p450 11beta-hydroxylase in the gonad of medaka (oryzias latipes). | alkylphenols including 4-tert-pentylphenol (4-pp) have been shown to alter sexual differentiation in fish due to their estrogenic properties. medaka (oryzias latipes) is so sensitive to these substances because morphological sex reversal and testis-ova induction are well developed in the exposed males. however, little work has been done to characterize the molecular effects of estrogenic substances on the morphological and gonadal feminization in male fish. cytochrome p450 11beta-hydroxylase (p4 ... | 2005 | 15642637 |
| the effect of para-nonylphenol on japanese eel (anguilla japonica) spermatogenesis in vitro. | endocrine disrupters have been recognized to interfere with endocrine systems that regulate reproduction, for example, by mimicking or inhibiting the action of endogenous sex steroid hormones including estradiol-17beta (e2). in the present study, we examined the effect of an endocrine disrupter, para-nonylphenol (p-np) on spermatogenesis, and compared it with the action of e2, using an eel testicular organ culture system. p-np alone stimulated early spermatogonial renewal in the same manner as e ... | 2005 | 15642638 |
| a quick method for species identification of japanese eel (anguilla japonica) using real-time pcr: an onboard application for use during sampling surveys. | to compensate for the limited number of morphological characteristics of fish eggs and larvae, we established a convenient and robust method of species identification for eggs of the japanese eel (anguilla japonica) using a real-time polymerase chain reaction (pcr) that can be performed onboard research ships at sea. a total of about 1.2 kbp of the mitochondrial 16s ribosomal rna gene sequences from all species of anguilla and 3 other anguilliform species were compared to design specific primer ... | 2004 | 15690101 |
| natriuretic peptide guanylyl cyclase receptors in the kidney of the japanese eel, anguilla japonica. | natriuretic peptides are linked to osmoregulation, cardiovascular and volume regulation in fishes. the peptides bind to two guanylyl-cyclase-linked receptors, natriuretic peptide receptor-a (npr-a) and npr-b, to elicit their effects. atrial natriuretic peptide (anp) binds principally to npr-a, whereas c-type natriuretic peptide (cnp) binds to npr-b. the teleost kidney has an important role in the maintenance of fluid and electrolyte balance; therefore, the location of npr-a and npr-b in the kidn ... | 2005 | 15789218 |
| roles of slc13a1 and slc26a1 sulfate transporters of eel kidney in sulfate homeostasis and osmoregulation in freshwater. | sulfate is required for proper cell growth and development of all organisms. we have shown that the renal sulfate transport system has dual roles in euryhaline eel, namely, maintenance of sulfate homeostasis and osmoregulation of body fluids. to clarify the physiological roles of sulfate transporters in teleost fish, we cloned orthologs of the mammalian renal sulfate transporters slc13a1 (nasi-1) and slc26a1 (sat-1) from eel (anguilla japonica) and assessed their functional characteristics, tiss ... | 2005 | 15802556 |
| a novel cysteine protease inhibitor with lectin activity from the epidermis of the japanese eel anguilla japonica. | a novel cysteine protease inhibitor (eel-cpi-1) was isolated from the epidermis of the eel. eel-cpi-1 was shown to bind strongly to both lactose- and carboxymethylated papain-affinity gels. its molecular mass under reducing condition was determined to be 18 kda by sds-polyacrylamide gel electrophoresis but approximately 30.5 kda under non-reducing-conditions. eel-cpi-1 inhibited papain (k(i)=18 nm) and ficin (k(i)=120 nm) competitively. combined with the data on amino acid and sequence analysis, ... | 2005 | 15820140 |
| "blood-contacting neurons" in the brain of the japanese eel anguilla japonica. | to discriminate "blood-contacting neurons" within the brain of the eel, evans blue (eb) was injected intraperitoneally. after five days, six brain areas were externally stained blue with the dye; the saccus dorsalis (sd), the epiphysis (e), the area postrema (ap), the posterior part of the magnocellular preoptic nucleus (pm), the pituitary (pit), and the saccus vasculosus (sv). among the eb-positive area, some cells in the pm, the anterior tuberal nucleus (nat) and the ap were discriminated as t ... | 2005 | 15828012 |
| androgen action on hepatic vitellogenin synthesis in the eel, anguilla japonica is suppressed by an androgen receptor antagonist. | involvement of additional hormones other than estrogen in the control of vitellogenin (vg) synthesis has been suggested in fish. however, no satisfactory explanation on the mechanism of the action of these hormones has been reported. in this study, we have exploited the possibility of androgen receptor mediation during the androgen action on the pathway of vg synthesis. hepatocytes were prepared from sexually immature japanese eel anguilla japonica and treated with estradiol-17beta (e2), 17alpha ... | 2005 | 15878656 |
| purification of follicle-stimulating hormone from immature japanese eel, anguilla japonica, and its biochemical properties and steroidogenic activities. | follicle-stimulating hormone (fsh) was purified, for the first time, from immature japanese eel, anguilla japonica, and its biochemical properties were investigated. fsh was extracted from immature eel pituitaries and purified by gel-filtration on sephadex g-100, and two step anion-exchange chromatography: stepwise elution on de-52, followed by gradient elution on tsk-gel super-q using hplc. purification was performed using its molecular mass and the positive reaction with anti-japanese eel (je) ... | 2005 | 15894317 |
| differential expression of neural and gonadal aromatase enzymatic activities in relation to gonadal development in japanese eel, anguilla japonica. | the objectives of the present study were to investigate the enzymatic characteristics of brain aromatase in japanese eel, anguilla japonica, as well as the correlations between aromatase activities in various tissues (brain regions, pituitary, and gonads) and ovarian development. eel brain aromatase exhibited a k(m) of 75 nm and a v(max) of 1.14 fmol/min mg protein (91.5 fmol/h mg protein), indicating an enzymatic activity much lower than in other teleosts but similar to that in mammals. this su ... | 2005 | 16106412 |
| first record of ostracods as natural intermediate hosts of anguillicola crassus, a pathogenic swimbladder parasite of eels anguilla spp. | the ostracod physocypria nipponica (ostracoda: candonidae) was found (prevalence 14.2%) to be the only intermediate host of the nematode anguillicola crassus (nematoda: anguillicolidae), a pathogenic swimbladder parasite of eels, in a greenhouse-heated culture pond at isshiki, aichi prefecture, japan. japanese eels anguilla japonica from the same pond were found to be infected by adult a. crassus (prevalence 71.8%, intensity 1 to 6). this indicates that a. crassus could complete its life cycle u ... | 2005 | 16231644 |
| steroidogenic activities of follicle-stimulating hormone in the ovary of japanese eel, anguilla japonica. | to clarify the physiological functions of follicle-stimulating hormone (fsh) during oogenesis in japanese eel, anguilla japonica, the steroidogenic activities of recombinant japanese eel fsh (rjefsh) were assessed in the eel ovary. female eel were injected with salmon pituitary homogenate to enhance the ovarian development, and the ovaries at different developmental stages were subjected to steroidogenic bioassay. these ovaries could be classified into three types according to oocyte growth and ... | 2006 | 16297918 |
| apolipoprotein complexity in japanese eel anguilla japonica: truncated apolipoprotein a-i and apolipoprotein a-i-like protein in plasma lipoproteins. | a truncated apolipoprotein (apo) a-i with a molecular weight (m(r)) of 26 kda was first isolated from the plasma high density lipoproteins of an atypical japanese eel (anguilla japonica). interestingly, this eel contained a very small amount of intact apoa-i (m(r)28 kda) in the plasma, although serine protease inhibitors were present throughout the plasma preparation. the n-terminal sequence of 20 amino acids in truncated apoa-i was completely identical with that of intact apoa-i. another apolip ... | 2005 | 16306716 |
| distribution of polychlorinated biphenyls in the water, sediment and fish from the pearl river estuary, china. | the concentrations and distribution of polychlorinated biphenyls were determined in water, surface sediments and fishes from the estuaries of pearl river, pr china. the results showed that the concentrations ranged from 2.47 ng l-1 to 6.75 ng l-1 in water, from 11.13 ng g-1 to 23.23 ng g-1 in sediments (dry weight) and from 68.64 ng g-1 to 316.85 ng-1 in fish (lipid weight). it was found that the feeding habits of fish were very significant in the accumulation of polychlorinated biphenyls and pa ... | 2005 | 16374930 |
| molecular cloning and gene expression of spo11 during spermatogenesis in the japanese eel, anguilla japonica. | spo11 is a protein involved specifically in the meiotic recombination in several species, however, it is little characterized in lower vertebrates. we identified a cdna encoding spo11 from the testis of japanese eel, anguilla japonica. the deduced amino acid sequence of eel spo11 was more than 60% identical with human and mouse spo11s. in order to examine changes in the expression and localization of spo11 during spermatogenesis induced by the injection of human chorionic gonadotropin (hcg) and ... | 2006 | 16426883 |
| molecular cloning and characterization of growth hormone receptor and its homologue in the japanese eel (anguilla japonica). | two cdnas encoding growth hormone receptor (ghr)-like genes, eghr1 and eghr2, were isolated from japanese eel (anguilla japonica) liver tissue. the putative eel ghr proteins showed conserved structural features of vertebrate ghrs, including six cysteine residues and a ygefs motif in the extracellular domain, a single transmembrane region, and proline-rich box 1 and box 2 domains. northern blot analysis showed a single eghr1 transcript in liver, while two sizes of eghr2 transcripts, thought to be ... | 2006 | 16458558 |