Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| on the mechanism of side chain oxidation of n-beta-alanyldopamine by cuticular enzymes from sarcophaga bullata. | the metabolism of n-beta-alanyldopamine (nbad) by sarcophaga bullata was investigated. incubation of nbad with larval cuticular preparations resulted in the covalent bindings of nbad to the cuticle and generation of n-beta-alanyl-norepinephrine (nbane) as the soluble product. when the reaction was carried out in presence of a powerful quinone trap viz., n-acetylcysteine, nbane formation was totally abolished; but a new compound characterized as nbad-quinone-n-acetylcysteine adduct was generated. ... | 1990 | 2134026 |
| allatostatic regulation of juvenile hormone production in vitro by the ring gland of drosophila melanogaster. | a factor(s) extracted from the third instar larval brains of the dipteran species sarcophaga bullata and drosophila melanogaster causes a dose-dependent reduction of juvenile hormone (jhb3) biosynthesis by isolated ring glands in vitro. in situ, this factor is presumably neuronally transmitted from the brain to ring gland. the allatostatic effect of the brain factor is reversible in vitro and may be overcome partially by the jhb3 precursor farnesoic acid. agents which act to increase the intrace ... | 1990 | 2155838 |
| mode of action of sapecin, a novel antibacterial protein of sarcophaga peregrina (flesh fly). | sapecin is an antibacterial protein purified from the culture medium of nih-sape-4, an embryonic cell line of sarcophaga peregrina [matsuyama, k. & natori, s. (1988) j. biol. chem. 236, 17112-17116]. as this protein inhibited the growth of gram-positive bacteria better than that of gram-negative bacteria, we studied its mode of action with special reference to its effects on s. aureus and escherichia coli. results showed that sapecin had high affinity for cardiolipin, which is a major phospholip ... | 1990 | 2172219 |
| 4-alkyl-o-quinone/2-hydroxy-p-quinone methide isomerase from the larval hemolymph of sarcophaga bullata. i. purification and characterization of enzyme-catalyzed reaction. | an enzyme which catalyzes the conversion of certain 4-alkyl-o-benzoquinones to 2-hydroxy-p-quinone methides has been purified to apparent homogeneity from the hemolymph of sarcophaga bullata by employing conventional protein purification techniques. the purified enzyme migrated with an approximate molecular weight of 98,000 on gel filtration chromatography. on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it migrated as a single band with a molecular weight of 46,000, indicating tha ... | 1990 | 2211605 |
| analysis of a gene cluster for sarcotoxin ii, a group of antibacterial proteins of sarcophaga peregrina. | sarcotoxin ii is a group of antibacterial proteins of sarcophaga peregrina (flesh fly) with related primary structures. we have cloned three genes in this family. these genes formed a tandem array with about 2-kb intervals, and one of them was present in the opposite strand. the putative amino acid sequences of the proteins encoded by these genes were very similar except for a deletion in one of them. all of the genes were found to be activated transiently in the same way when the larval body wa ... | 1990 | 2247051 |
| production of recombinant sarcotoxin ia in bombyx mori cells. | a cdna for sarcotoxin ia, an antibacterial protein of sarcophaga peregrina (fleshfly), was inserted into a silkworm baculovirus vector and expressed in bm-n cells, a line of bombyx mori cells. when a cysteine proteinase inhibitor, p-chloromercuribenzenesulphonic acid, was present in the culture medium, a significant amount of recombinant sarcotoxin ia accumulated, but without this reagent the product seemed to be degraded in this system. the c-terminus of the recombinant sarcotoxin ia seemed to ... | 1990 | 2268291 |
| immunocytochemical localization in the central nervous system of four different insect species of molecules immunoreactive against peptides present in the caudodorsal cells of lymnaea stagnalis. | the use of polyclonal antisera directed against three peptides (ovulation hormone (cdch), alpha, and beta caudodorsal cell peptide (alpha- and beta-cdcp) produced by the caudodorsal cells of the snail lymnaea stagnalis resulted in positive immunoreaction in sarcophaga bullata (diptera), leptinotarsa decemlineata (coleoptera), locusta migratoria, and periplaneta americana (orthoptera). in three species, colocalization was detected using the antisera against cdch and alpha-cdcp. | 1990 | 2272462 |
| preliminary observations on myiasis in leprosy patients. | out of 3350 leprosy patients attending the surgical outpatient department for various ulcerative lesions, 18 patients had typical symptoms of myiasis. maggots were collected in 5 cases from the nose, in 3 cases from ulcers of the hand and in 10 cases from ulcers of the foot. it was possible to rear the maggots into flies in 8 out of 18 cases. the flies were identified as sarcophaga ruficornis and chrysomyia bezziana. | 1990 | 2280659 |
| detection of extracellular calcium gradients with a calcium-specific vibrating electrode. | we have developed a vibrating calcium-specific electrode to measure minute extracellular calcium gradients and thus infer the patterns of calcium currents that cross the surface of various cells and tissues. low-resistance calcium electrodes (routinely approximately 500 m omega) are vibrated by means of orthogonally stacked piezoelectrical pushers, driven by a damped square wave at an optimal frequency of 0.5 hz. phase-sensitive detection of the electrode signal is performed with either analogue ... | 1990 | 2335563 |
| characterization of a tandemly repeated dna from the fleshfly sarcophaga bullata. | in studies on the highly repetitive dna sequences of the flesh fly sarcophaga bullata, a 279 bp tandem repeat was cloned and sequenced. a 17 bp stretch within the clone was identical to a motif repeated five times in the satellite dna of the bermuda land crab. southern dna blotting showed the tandem repeat had a high degree of conservation of mboi sites, but had divergence for ecori sites; thus, all repeat units were not identical. the cloned dna localized to the quinacrine-bright centromeric he ... | 1990 | 2357913 |
| determination of the disulfide array in sapecin, an antibacterial peptide of sarcophaga peregrina (flesh fly). | sapecin is a 40-residue peptide containing 6 half-cystine residues. the disulfide structure of sapecin was determined by sequencing cystine-containing peptides obtained by digesting sapecin with thermolysin. results showed that sapecin has a vortical structure fixed by 3 disulfide bonds between cysteine residues 3 and 30, 16 and 36, and 20 and 38, respectively, and that these disulfide bonds are essential for its antibacterial activity. | 1990 | 2358424 |
| a potent antibacterial protein in royal jelly. purification and determination of the primary structure of royalisin. | a new potent antibacterial protein, for which we propose the name royalisin, was found in royal jelly of the honeybee apis mellifera l. and purified to homogeneity for the first time by acid extraction, gel filtration, and reverse-phase high pressure liquid chromatography. the primary structure of royalisin was determined to consist of 51 residues, with three intramolecular disulfide linkages, having a calculated molecular mass of 5523 da. royalisin is an amphipathic protein, with the c-terminal ... | 1990 | 2358464 |
| 1h nuclear magnetic resonance study of the solution conformation of an antibacterial protein, sapecin. | the solution conformation of an antibacterial protein sapecin has been determined by 1h nuclear magnetic resonance (nmr) and dynamical simulated annealing calculations. it has been shown that the polypeptide fold consists of one flexible loop (residues 4-12), one helix (residues 15-23), and two extended strands (residues 24-31 and 34-40). it was found that the tertiary structure of sapecin is completely different from that of rabbit neutrophil defensin np-5, which is homologous to sapecin in the ... | 1990 | 2401368 |
| cloning and sequencing of cdna of sarcophaga peregrina humoral lectin induced on injury of the body wall. | a previous paper described the purification of a lectin induced in the hemolymph of larvae of sarcophaga peregrina (flesh-fly) on injury of their body wall (komano, h., mizuno, d., and natori, s. (1980) j. biol. chem. 255, 2919-2924). this paper describes cdna cloning and the complete nucleotide sequence of the gene for sarcophaga lectin. although active lectin consists of alpha and beta subunits in a molar ratio of 2:1, the fat body of injured larvae was found to contain only mrna for the alpha ... | 1985 | 2413021 |
| participation of common surface receptor(s) in the activation of murine macrophages by sarcophaga lectin and wheat germ agglutinin. | mouse peritoneal macrophage surface proteins which bind sarcophaga lectin were studied. two major binding proteins with molecular masses of 170 and 110 kda were identified. sarcophaga lectin and wheat germ agglutinin were found to share common binding proteins for activating macrophages, although their hapten sugars are different. antibody raised against the sarcophaga lectin-binding proteins inhibited both the production of tumor-specific cytotoxic protein by the macrophage-like cell line j774. ... | 1986 | 2423233 |
| polyploidization and localisation of poly(a)+ rna in the different cell types of the vitellogenic meroistic ovary of the fleshfly, sarcophaga bullata. | the degree of polyploidization, the level of transcriptional activity and the volume of the different cell types present in the meroistic ovary of sarcophaga bullata were measured during different vitellogenic stages. the nurse cells and the germinal vesicle exhibited very pronounced differences with regard to dna content and mrna synthesis, even though they are genetically identical. during the 4c stage (late vitellogenesis), we observed different degrees of polyploidy in follicle cells adjacen ... | 1986 | 2428776 |
| molecular cloning, sequencing, and characterization of cdna for sarcotoxin iia, an inducible antibacterial protein of sarcophaga peregrina (flesh fly). | a cdna clone for sarcotoxin iia, an antibacterial protein of sarcophaga peregrina (flesh fly) larvae [ando, k., okada, m., & natori, s. (1987) biochemistry 26, 226-230], was isolated and characterized. sarcotoxin iia was found to consist of 270 amino acid residues. northern blot analysis showed that the sarcotoxin iia gene was activated in response to injury of the body wall of the larvae. the gene was activated for much longer after injection of escherichia coli into the abdominal cavity of lar ... | 1988 | 2452654 |
| structure and expression of a manduca sexta larval cuticle gene homologous to drosophila cuticle genes. | a genomic clone was isolated from the tobacco hornworm, manduca sexta, by virtue of its similarity to a drosophila larval cuticle gene. rna analysis shows that this clone, b311, is expressed at times appropriate for a larval cuticle gene. hybrid-selection experiments using b311 dna show that it encodes a 14 x 10(3) mr protein, lcp-14, which is precipitated by an antiserum to manduca larval cuticle. we have sequenced both genomic and cdna clones for the lcp-14 gene. a conceptual translation of th ... | 1988 | 2462055 |
| cloning and in vitro transcription of the sarcophaga lectin gene. | a genomic clone for the sarcophaga lectin gene was isolated. this gene was a compact single copy gene. two transcription initiation sites were located by s1 nuclease mapping and primer extension. however, transcription from one of these initiation sites was much greater than that from the other site under all conditions in which this gene was expressed. this gene was found to be transcribed efficiently in a nuclear extract of nih-sape-4 cells, an embryonic cell line of sarcophaga synthesizing sa ... | 1989 | 2480809 |
| o-quinone/quinone methide isomerase: a novel enzyme preventing the destruction of self-matter by phenoloxidase-generated quinones during immune response in insects. | melanization and encapsulation of invading foreign organisms observed during the immune response in insects is known to be due to the action of activated phenoloxidase. phenoloxidase-generated quinones are deposited either directly or after self-polymerization on foreign objects accounting for the observed reactions. since the reactions of quinones are nonenzymatic, they do not discriminate self from nonself and hence will also destroy self-matter. in this report we present evidence for the pres ... | 1989 | 2500362 |
| n-acetyldopamine quinone methide/1,2-dehydro-n-acetyl dopamine tautomerase. a new enzyme involved in sclerotization of insect cuticle. | the enzyme system causing the side chain desaturation of the sclerotizing precursor, n-acetyldopamine (nada), was solubilized from the larval cuticle of sarcophaga bullata and resolved into three components. the first enzyme, phenoloxidase, catalyzed conversion of nada to nada quinone and provided it for the second enzyme (nada quinone isomerase), which makes the highly unstable nada quinone methide. quinone methide was hydrated rapidly and nonenzymatically to form n-acetylnorepinephrine. in add ... | 1989 | 2507358 |
| cytochemical studies of oocyte development in sarcophaga lineatocollis macq. (muscidae: diptera). | the ovary of sarcophaga lineatocollis is a typical polytrophic ovary. each of its 25-30 ovarioles is composed of a small terminal filament, a small germarium and a vitellarium consisting of the egg follicle. the tunica propria is a noncellular, pas-positive membrane. the ovarian follicle contains fifteen trophocytes and one oocyte. rna is synthesized with the aid of the nuclei in the trophocyte cytoplasm, which are rna- and pas-positive. protein is deposited intensively in the early stages of th ... | 1989 | 2606383 |
| recovery of forensically important entomological specimens from human cadavers in malaysia--an update. | a total of 101 entomological specimens recovered from human cadavers were processed and studied. analysis of the data indicated that about 95% of these specimens were maggots of flies. maggots of the blowfly chrysomya (family: calliphoridae) especially ch. rufifacis and ch. megacephala were predominantly found in 77 cases (76.2%) while larvae of several other flies of the genera sarcophaga, calliphora, lucilia and hermetia were also recovered. it was notable that musca domestica or other related ... | 1989 | 2632998 |
| cloning of gene cluster for sarcotoxin i, antibacterial proteins of sarcophaga peregrina. | a genomic clone of sarcotoxin i was isolated. this clone contained four genes of structurally related proteins belonging to the sarcotoxin i family present in tandem array. one of these genes was sequenced and found to be the sarcotoxin ib gene. this gene contained a single intron of 95 bases. | 1989 | 2689214 |
| immunohistochemical localisation of the hypertrehalosaemic hormone ii (cam-hrth-ii) and related peptides in the nervous system of carausius morosus and sarcophaga bullata. | a polyclonal antiserum was prepared against an n-terminal modified cam-hrth-ii (leu-asn-phe-...), one of the members of the large akh/rpch peptide family, first isolated from carausius morosus. the localisation of this peptide was performed by means of immunocytochemical methods in the brain and corpora cardiaca-corpora allata complex of the stick insect, carausius morosus and the grey fleshfly, sarcophaga bullata. the distribution patterns of molecules reactive to the cam-hrth-ii and the lom-ak ... | 1989 | 2692834 |
| models for positional signalling with application to the dorsoventral patterning of insects and segregation into different cell types. | models of pattern formation and possible molecular realizations are discussed and compared with recent experimental observations. in application to the dorsoventral patterning of insects, it is shown that a superposition of two pattern-forming reactions is required. the first system generates the overall dorsoventral polarity of the oocyte, the second generates the positional information proper with a stripe-like region of high concentration along the ventral side of the embryo. a single reactio ... | 1989 | 2699854 |
| stage-specific detection of a dna-binding protein for the storage protein gene of sarcophaga peregrina. | a nuclear extract of fat body prepared from third instar larvae of sarcophaga peregrina (flesh fly) was found to contain a dna-binding protein that specifically bound to 5'-upstream region of the storage protein (arylphorin) gene. this protein was found only in larvae harvested 46 h after larval emergence, indicating that its appearance was strictly regulated by the developmental stage of the insect. since the storage protein gene is actively transcribed in the fat body at this stage, this prote ... | 1989 | 2719963 |
| characterization of a new enzyme system that desaturates the side chain of n-acetyldopamine. | a novel enzyme system that desaturates the side chain of the catecholamine derivative, n-acetyldopamine (nada), was isolated and characterized from the larval cuticle of sarcophaga bullata. the nada desaturase system which converts nada to 1,2-dehydro-nada, surprisingly, does not resemble dehydrogenases such as succinate dehydrogenase. it uniquely performs the desaturation reaction by oxidizing nada to its corresponding quinone and subsequently converting the resultant quinone to 1,2-dehydro-nad ... | 1989 | 2753165 |
| postembryonic development of wohlfahrtia magnifica (schiner, 1862) (diptera: sarcophagidae). | a description of the larval development of wohlfahrtia magnifica (schiner, 1862) is given. an interpretation of the morphological development correlated with functional and adaptive aspects of myiasis-causing flies is also included. a detailed analysis of morphological changes that occurred in w. magnifica larvae during postembryonic development is carried out. these changes happen in 2 larval interinstars, i-ii and ii-iii, in which several structures such as anterior and posterior peritremes an ... | 1989 | 2760766 |
| molecular cloning of cdna for sarcocystatin a and analysis of the expression of the sarcocystatin a gene during development of sarcophaga peregrina. | sarcocystatin a is a cysteine proteinase inhibitor purified from the hemolymph of sarcophaga peregrina larvae [suzuki, t., & natori, s. (1985) j. biol. chem. 260, 5115-5120]. we isolated a cdna clone for sarcocystatin a and analyzed the structure and expression of the sarcocystatin a gene. sarcocystatin a consists of 102 amino acid residues. significant homology was found between amino acid sequences of sarcocystatin a and other mammalian cystatins, and highly conserved sequences among mammalian ... | 1989 | 2785815 |
| characterization and origin of extrachromosomal dna granules in sarcophaga bullata. | we have used endonuclease treatment in situ, followed by giemsa or ethidium bromide staining, for mapping repetitive sequences on the chromosomes of the flesh fly sarcophaga bullata and thus for studying extrachromosomal dna granules in this species. all three restriction enzymes employed (haeiii, a1ui and hindiii) show the same cytological effects, except for a single interstitial band. in both polytene and mitotic chromosomes, chromatin resistant to these endonucleases presumably includes at l ... | 1987 | 2834406 |
| the role of some cyclorrhaphan flies as carriers of human helminths in malaysia. | the role of some adult flies (diptera: cyclorrhapha) as carriers of helminth parasites of man was studied at four sites in malaysia: a refuse dump, where no helminth-positive flies were detected, and in three peri-domestic situations where four species of flies carried up to three types of nematodes. the dominant fly species chrysomya megacephala (fabricius) carried eggs of the roundworm ascaris lumbricoides l., the pinworm trichuris trichiura (l.) and hookworm on the adult external body surface ... | 1988 | 2980156 |
| structural analysis of a developmentally regulated 25-kda protein gene of sarcophaga peregrina. | in the previous paper, we described the identification of two abundant mrnas of sarcophaga peregrina (flesh-fly) which are selectively expressed in the fat body of middle third instar larvae. one of these mrnas was found to encode a protein with a molecular mass of about 25,000 (25-kda protein) when translated in vitro (tamura, h., et al. (1983) dev. biol. 99, 145-151). present paper reports the nucleotide sequence of a 2.3 kb dna containing the entire gene for the 25-kda protein. this gene cons ... | 1985 | 2993269 |
| possible involvement of lumichrome in the binding of storage protein to its receptor in sarcophaga peregrina. | previously, a receptor molecule on the surface of fat body cells of sarcophaga peregrina larvae that is involved in the uptake of storage protein from the hemolymph was shown to be a 120-kda protein (ueno, k., and natori, s. (1984) j. biol. chem. 259, 12107-12111). this paper reports evidence that lumichrome (7,8-dimethylalloxazine) may be present in the binding site of the receptor and mediate the binding of storage protein and receptor. a stoichiometric amount of lumichrome was shown to bind t ... | 1987 | 3040764 |
| inhibitory effect of sarcotoxin iia, an antibacterial protein of sarcophaga peregrina, on growth of escherichia coli. | the effect of sarcotoxin iia, an antibacterial protein of sarcophaga peregrina (flesh fly), on escherichia coli was investigated. sarcotoxin iia was found to have a bacterial effect on growing bacteria, but little on non-growing bacteria. at a concentration of 25 micrograms/ml, it induced significant morphological change of growing e. coli cells. in its presence, growing cells became greatly elongated, and spheroplast-like bulges and projections appeared on their surface. a rough mutant strain o ... | 1988 | 3049566 |
| purification of sarcotoxin ii, antibacterial proteins of sarcophaga peregrina (flesh fly) larvae. | three antibacterial proteins with almost identical primary structures termed sarcotoxin iia, iib, and iic were purified to homogeneity from the hemolymph of third instar larvae of sarcophaga peregrina. the molecular masses of these proteins were about 24,000. these proteins were found to have common antigenicity, and antibody against sarcotoxin iia cross-reacted with sarcotoxin iib and iic. radioimmunoassay using this antibody showed that these proteins are induced in the hemolymph in response t ... | 1987 | 3103678 |
| purification of three antibacterial proteins from the culture medium of nih-sape-4, an embryonic cell line of sarcophaga peregrina. | three antibacterial proteins were purified from the culture medium of nih-sape-4, an embryonic cell line of sarcophaga peregrina (flesh fly). sequencing studies showed that two of these proteins belong to the sarcotoxin i family, potent antibacterial proteins purified from the hemolymph of sarcophaga larvae, whereas the other protein, named sapecin, is a new protein consisting of 40 amino acid residues including 6 cysteine residues. unlike sarcotoxin i, sapecin preferentially represses the growt ... | 1988 | 3182836 |
| molecular cloning of cdna for sapecin and unique expression of the sapecin gene during the development of sarcophaga peregrina. | a cdna clone for sapecin, an antibacterial protein produced by an embryonic cell line of sarcophaga peregrina, was isolated and characterized. this clone was found to encode a precursor of sapecin consisting of 94 residues, with sapecin (40 residues) constituting its carboxyl-terminal half. rna blot hybridization revealed that the gene for the sapecin precursor is activated in the hemocytes of the third instar larvae of sarcophaga in response to body injury. thus, sapecin is probably a defense p ... | 1988 | 3182837 |
| differential polarization microscopy (cd and linear dichroism) of polytene chromosomes and nucleoli from the dipteran sarcophaga footpad. | 1988 | 3233329 | |
| npy-like peptides occur in the nervous system and midgut of the migratory locust, locusta migratoria and in the brain of the grey fleshfly, sarcophaga bullata. | the distribution of the npy-like substances in the nervous system and the midgut of the migratory locust, locusta migratoria and in the brain of the grey fleshfly, sarcophaga bullata was determined by immunocytochemistry using an antiserum directed against synthetic porcine npy. the peroxidase-antiperoxidase procedure revealed that npy immunoreactive cell bodies and nerve fibers were observed in the brain, optic lobes, corpora cardiaca, suboesophageal ganglion and ventral nerve cord of the locus ... | 1988 | 3244556 |
| purification of sarcotoxin iii, a new antibacterial protein of sarcophaga peregrina. | a glycine-rich antibacterial protein with a molecular mass of 7,000 termed sarcotoxin iii, was purified to homogeneity from the hemolymph of third instar larvae of sarcophaga peregrina. when the hemolymph was fractionated, this protein was recovered in the same fraction as sarcotoxin i, a group of potent antibacterial proteins that have been purified. but, it was clearly different from sarcotoxin i in amino acid composition and molecular mass. sarcotoxin iii was shown to be induced in the hemoly ... | 1987 | 3312185 |
| novel feature of expression of the sarcotoxin ia gene in development of sarcophaga peregrina. | the expressions of the sarcotoxin ia and iia genes were investigated. although both these antibacterial proteins were synthesized by the fat body and secreted into the hemolymph in the same way when the body wall of third instar larvae of sarcophaga peregrina was injured, the expressions of their genes during development of this insect were different. the sarcotoxin ia gene was activated transiently in the embryonic and pupal stages, whereas the sarcotoxin iia gene was totally inactive in these ... | 1988 | 3342034 |
| effects on electrophoretic mobility and antibacterial spectrum of removal of two residues from synthetic sarcotoxin ia and addition of the same residues to cecropin b. | cecropin b and cecropin ia (sarcotoxin ia) are 35- and 39-residue antibacterial peptides from a silk moth and a meat fly, respectively. using solid phase synthesis we have made these peptides as well as two 37-residue analogs, one containing a deletion of leucine and lysine (residues 2a and 2b) as compared to cecropin ia, the other containing an insertion of leucine and lysine at the corresponding place in cecropin b. this addition and removal of a lysine residue did not cause the expected chang ... | 1988 | 3360136 |
| methionine-enkephalin immunoreactivity in the gonads and nervous system of two insect species: locusta migratoria and sarcophaga bullata. | methionine(met)-enkephalin immunoreactivity as visualized by the peroxidase-antiperoxidase procedure, is present in spermatogonia, spermatocytes, spermatids, and young ovarian follicles of locusta (panoistic type) and sarcophaga (polytrophic type). follicle cells and mature spermatozoa are always immunonegative as are locust vitellogenic follicles. in oocytes and in trophocytes, the met-enkephalin-like material first appears around the nucleus and is then dispersed throughout the cytoplasm. late ... | 1988 | 3360284 |
| humoral mediator-dependent activation of the sarcophaga lectin gene. | sarcophaga lectin is a defence protein synthesized by the fat body and secreted into the hemolymph in response to injury of the body of third instar larvae of sarcophaga peregrina (flesh-fly). in this paper, we demonstrate that the stimulus of body injury is first transmitted to a certain tissue present in the anterior part of the body, and from there a mediator molecule that interacts directly with fat body cells is secreted into the hemolymph. on interaction with this mediator molecule, the fa ... | 1988 | 3366243 |
| identification and characterization of sarcophaga lectin receptor on the surface of murine macrophages by use of monoclonal antibodies. | the structure of sarcophaga lectin receptor on the surface of murine macrophages was analyzed using monoclonal antibodies. this receptor was found by gel filtration to have a molecular weight of 460 kda. sds-polyacrylamide gel electrophoresis showed that this receptor consists of two subunits of 170 kda and 110 kda. the results indicated that it is probably a heterotetramer of two molecules of each subunit. two monoclonal antibodies recognized epitopes in the 110 kda subunit, and one of them spe ... | 1988 | 3391994 |
| purification of sarcophaga (fleshfly) lectin and detection of sarcotoxins in the culture medium of nih-sape-4, an embryonic cell line of sarcophaga peregrina. | an established cell line originating from a sarcophaga peregrina (fleshfly) embryo, nih-sape-4, was found to synthesize mrnas for sarcophaga lectin and sarcotoxin ia, but not those for storage protein or 25 kda protein. these four proteins are known to be synthesized in the fat-body of third-instar larvae, and the two former in particular are known to participate in the defence mechanism of this insect and to be induced in response to injury of the body wall. thus the embryonic cell line nih-sap ... | 1987 | 3435439 |
| distribution of yolk polypeptides in the follicle cells during the differentiation of the follicular epithelium in sarcophaga bullata egg follicles. | in s. bullata, the ovaries contribute to the synthesis of yolk polypeptides. a specific antiserum for yolk polypeptides was used to visualize the presence of yolk polypeptides in the follicle cells during their differentiation. after vitellogenesis has started, all follicle cells contain yolk polypeptides. the squamous follicle cells covering the nurse cells and the border cells lose yolk polypeptides before mid-vitellogenesis, whereas the follicle cells over the oocyte contain yolk polypeptides ... | 1987 | 3449367 |
| a case of intestinal myiasis in japan. | a case of intestinal myiasis caused by larvae of sarcophaga peregrina (japanese name: senchi-nikubae) is reported. the patient passed stools containing live fly larvae for 2 days, but was otherwise asymptomatic. the patient seems to have been infested accidentally as a result of having eaten "yakitori" in which the fly had deposited its larvae. this paper is the first report of intestinal myiasis caused by the 3rd instar of this species. | 1987 | 3508658 |
| interaction between liposomes and sarcotoxin ia, a potent antibacterial protein of sarcophaga peregrina (flesh fly). | the direct interaction between phospholipids and sarcotoxin ia, a potent bactericidal protein of sarcophaga peregrina, was studied using authentic sarcotoxin ia, its synthetic derivatives, and various liposomes. results showed that sarcotoxin ia interacted with liposomes constituted from acidic phospholipids, resulting in the release of glucose trapped in these liposomes. the amidated carboxyl-terminal of this protein was found to be important for this interaction. liposomes constituted from tot ... | 1987 | 3543008 |
| [biochemistry of sarcophaga peregrina (flesh fly)]. | 1986 | 3546526 | |
| localization of melanotropin-like peptides in the central nervous system of two insect species, the migratory locust, locusta migratoria, and the fleshfly, sarcophaga bullata. | by use of well characterized antisera in the peroxidase-antiperoxidase method, we were able to demonstrate alpha msh and beta msh immunoreactive cells and nerve fibres within the nervous system of adults and larvae of locusta migratoria and 3-, 5- and 8-day-old adult sarcophaga bullata. in neither of these insect species, any immunoreaction was obtained with a gamma 3msh-antiserum. double immunohistochemical stainings revealed that alpha msh-like and beta msh-like substances are located in diffe ... | 1987 | 3552238 |
| counteraction by 20-hydroxyecdysone of the effect of juvenile hormone on phosphorylation of ribosomal protein s6. | phosphorylation of ribosomal protein s6, induced when the fat body of sarcophaga peregrina (flesh-fly) larvae was incubated in vitro in the presence of 32p, was found to be suppressed by juvenile hormone. this suppressive effect of juvenile hormone was counteracted by a physiological concentration of 20-hydroxyecdysone. since 20-hydroxyecdysone is known to induce phosphorylation of s6 in vivo, this reaction was suggested to be regulated by the balance of the effects of ecdysone and juvenile horm ... | 1987 | 3556581 |
| the complete amino acid sequence of echinoidin, a lectin from the coelomic fluid of the sea urchin anthocidaris crassispina. homologies with mammalian and insect lectins. | the complete amino acid sequence of echinoidin, the proposed name for a lectin from the coelomic fluid of the sea urchin anthocidaris crassispina, has been determined by sequencing the peptides obtained from tryptic, staphylococcus aureus v8 protease, chymotryptic, and thermolysin digestions. echinoidin is a multimeric protein (giga, y., sutoh, k., and ikai, a. (1985) biochemistry 24, 4461-4467) whose subunit consists of a total of 147 amino acid residues and one carbohydrate chain attached to s ... | 1987 | 3571253 |
| rapid inactivation of tumor killing factor in vitro. | the mechanism of the rapid inactivation of the cytotoxic activity produced by peritoneal macrophages stimulated with sarcophaga lectin was investigated. experiments using radioiodinated tumor killing factor (tkf) showed that the main reason for its inactivation was the rapid dissociation of its trimeric structure into 15-kda subunits. this dissociation was found to proceed spontaneously in the culture medium irrespective of the presence of macrophages. | 1987 | 3584087 |
| crossbridges in insect flight muscles of the blowfly (sarcophaga bullata). | crossbridges in quick-frozen deep-etched blowfly flight muscles (from sarcophaga bullata) were compared with those observed in the traditional waterbug preparation (lethocerus) and found to be indistinguishable. hence, blowfly was chosen as a fresher more accessible tissue for determining the effect of various fixatives and nucleotides on crossbridge structure. in rigor control, crossbridges were most regular in muscles that were stabilized before freezing by prefixation in glutaraldehyde follow ... | 1987 | 3654957 |
| study of the metabolism of steroids in larvae of the fleshfly sarcophaga bullata. | 1. larvae of the fleshfly sarcophaga bullata were injected with several 3h c21 and c19 steroids. after different incubation times, the larvae were homogenized and the metabolites were extracted and fractionated by sephadex lh 20-, paper- and thin-layer chromatography. the chromatographic mobility of the labeled zones was compared with that of standard steroids. 2. progesterone and 17 alpha-hydroxypregnenolone were metabolized to 17 alpha-hydroxyprogesterone. androstenedione, 17 alpha-hydroxyprog ... | 1987 | 3665429 |
| many circadian oscillators regulate developmental and behavioural events in the flesh-fly, sarcophaga argyrostoma. | in the flesh-fly, sarcophaga argyrostoma, the initiation of larval wandering, pupal eclosion, and the induction of pupal diapause by seasonal changes in night length, are all regulated by circadian oscillators. they differ, however, in several respects. the rhythm of larval wandering shows a free-running period (tau) of about 20 hr and a steady-state phase-relationship to the light cycle (psi) in which maximum activity occurs at dusk or in the night; that for pupal eclosion shows tau close to 24 ... | 1986 | 3677196 |
| heat shock responses in polytene foot pad cells of sarcophaga bullata. | heat shock induces a single large puff (hs puff) near the tip of chromosome arm el in polytene foot pad cells of fly pupae (sarcophaga bullata). the inducible hs locus is constitutively active, invariably forming a small puff, which can be maximally activated in cells of the dorsal epidermis or in trichogen cells at any time during the lifetime of mature polytene chromosomes. both in vivo and in cultured food pads, maximal puff induction occurs at 37 degrees c. at the same temperature, normal de ... | 1986 | 3698748 |
| induction of a heat shock puff by hypoxia in polytene foot pad chromosomes of sarcophaga bullata. | the single large heat-responsive puff (hs puff) in polytene foot pad cells of fly pupae (sarcophaga bullata) is shown to be inducible by oxygen deprivation but not, as in other systems, by reoxygenation following an hypoxic treatment. the ambient oxygen concentration must drop below 2% for the hs puff to be maximally induced but the puff is fully inducible and transcriptionally active even in the complete absence of oxygen. lack of oxygen is also compatible with continued transport of puff mater ... | 1986 | 3698749 |
| histochemical and cytological changes in development of the oocyte of sugar-fed females and the resorptive bodies of sarcophaga lineatocollis (macq.). | 1986 | 3699643 | |
| myiatic scalp and skull infection with diptera sarcophaga: case report. | the authors describe an unusual infestation of an ulcerative squamous cell carcinoma of the scalp and skull with diptera sarcophaga larvae. human myiasis is rare in temperate zones and presents a rather unique neurosurgical challenge. the clinical presentation and treatment of human myiasis are briefly discussed. | 1986 | 3703198 |
| participation of tumor killing factor in the antitumor effect of sarcophaga lectin. | antibody against tumor-killing factor inhibited cytotoxic activities produced in vitro by mouse peritoneal macrophages and in vivo in the serum of tumor-bearing mice in response to sarcophaga lectin. these results suggest that tumor-killing factor participates in the antitumor effect of sarcophaga lectin. | 1986 | 3709806 |
| histochemical and cytological observations on the developing ovary of sarcophaga lineatocollis macq. (diptera-muscidae). | 1986 | 3744181 | |
| identification of 30-kda fat body protein of sarcophaga peregrina larvae selectively phosphorylated in the presence of 20-hydroxyecdysone as ribosomal protein s6. | previously, we showed that 20-hydroxyecdysone induces selective phosphorylation of a fat body protein of sarcophaga peregrina with a molecular mass of about 30,000 (30-kda protein) (itoh, k., ueno, k., & natori, s. (1985) biochem. j. 227, 683-688). this paper describes the identification of this 30-kda protein. from the electrophoretic profile of 40s ribosomal proteins on two-dimensional polyacrylamide gel electrophoresis, the 30-kda protein was identified as s6. | 1986 | 3782062 |
| ring gland and prothoracic gland sensitivity to interspecific prothoracicotropic hormone extracts. | using the techniques of intraspecific in vitro activation of prothoracic glands and ring glands by serial dilutions of prothoracicotropic hormone (ptth) extracts from pupal manduca sexta (lepidoptera) and larval sarcophaga bullata (diptera), a dose-response of activation was observed for both species. in both species maximum activation was at 0.5 brain equivalents while the number of brain equivalents necessary for half maximal stimulation (ed50) was 0.20 for manduca and 0.15 for sarcophaga. whe ... | 1986 | 3794015 |
| molecular cloning of a cdna and assignment of the c-terminal of sarcotoxin ia, a potent antibacterial protein of sarcophaga peregrina. | a previous paper described the complete amino acid sequences of sarcotoxins ia, ib and ic, which are a group of potent antibacterial proteins with almost identical primary structures produced by sarcophaga peregrina (fleshfly) larvae [okada & natori (1985) j. biol. chem. 260, 7174-7177]. the present paper describes the cdna cloning and complete nucleotide sequencing of a cdna clone for sarcotoxin ia. the c-terminal amino acid residue of sarcotoxin ia deduced from the nucleotide sequence was glyc ... | 1986 | 3827823 |
| purification and characterization of an inhibitor of the cysteine protease from the hemolymph of sarcophaga peregrina larvae. | the hemolymph of sarcophaga peregrina (flesh fly) larvae was found to contain multiple inhibitors of hemocyte cysteine protease. one of them, named sarcocystatin a, was purified and found to be a mixture of the components sarcocystatin a alpha and a beta in a molar ratio of 2:1. these components can exist in either the associated or dissociated form. the apparent heterogeneity of the protease inhibitors in the hemolymph was found to be partly due to association of sarcocystatin a alpha and a bet ... | 1985 | 3886650 |
| primary structure of sarcotoxin i, an antibacterial protein induced in the hemolymph of sarcophaga peregrina (flesh fly) larvae. | the primary structure of sarcotoxin i, a potent bactericidal protein induced in the hemolymph of larvae of sarcophaga peregrina (flesh fly), was investigated. sarcotoxin i was a mixture of three proteins (sarcotoxins ia, ib, and ic) with almost identical primary structures. these proteins were found to consist of 39 amino acid residues and to differ in only 2-3 amino acid residues. the amino-terminal half of the molecules was rich in charged amino acids and was hydrophilic, whereas the carboxyl- ... | 1985 | 3888997 |
| ionophore activity of sarcotoxin i, a bactericidal protein of sarcophaga peregrina. | when escherichia coli was treated with sarcotoxin i, a potent bactericidal protein of sarcophaga peregrina (fleshfly), k+ inside of the cells leaked out rapidly and the atp pool of the cells rapidly decreased. these results suggested that the bactericidal effect of sarcotoxin i was due to its ionophore activity, and that it blocked the generation of atp by inhibiting formation of the proton gradient essential for oxidative phosphorylation. this was confirmed by use of an unca mutant, which was m ... | 1985 | 3899107 |
| sarcophagid larval proteins: partial sequence homologies among three cuticle proteins and related structures of drosophilids. | three structural proteins from the larval cuticle of sarcophaga bullata have been sequenced at the amino terminus for 30-40 residues. we observed a high degree of homology with related proteins of drosophila melanogaster, based on the previous findings of m. snyder, j. hirsh, and n. davidson [(1981) cell 25:165-177]. s. bullata protein sc1 had 65% homology with drosophila isolate cp1, and sc6 showed 49% homology with cpx and 54% with cp2a. the three sarcophagid polypeptides also resembled each o ... | 1985 | 3932663 |
| endogenous protease inhibitors prevent undesired activation of prophenolase in insect hemolymph. | phenoloxidase activation in the whole hemolymph of sarcophaga bullata and manduca sexta larvae is shown to be achieved by proteolytic cleavage of the proenzyme. this process is inhibited by the serine protease inactivator, diisopropyl phosphofluoridate. endogenous protease inhibitors isolated from the larvae inhibit alpha-chymotrypsin mediated prophenoloxidase activation in the hemolymph. these observations suggest that the endogenous protease inhibitors prevent undesired activation of prophenol ... | 1985 | 3935107 |
| antitumor effect of sarcophaga lectin on murine transplanted tumors. | sarcophaga lectin, a lectin purified from the hemolymph of sarcophaga peregrina (flesh fly) larvae, was found to be therapeutically effective against both ascitic and solid tumors. it was especially effective when injected directly into or around nodules of the syngeneic tumor meth a transplanted intradermally into balb/c mice. it also induced cytotoxic activity against l-929 cells when it was added to the culture medium of macrophages in vitro. similar cytotoxic activity was detected transientl ... | 1985 | 3935621 |
| analysis of the dnase i-hypersensitive site of a developmentally regulated 25-kda protein gene of sarcophaga peregrina. | change in chromatin structure of a developmentally regulated gene of sarcophaga peregrina (flesh fly) during development was investigated. this gene (25-kda protein gene) was specifically activated in the fat body, but not the hemocytes of larvae in the middle of the third instar. the mrna level in the fat body decreased thereafter, reaching one-fifth of the maximum level in the late third instar to early pupal stage. in the chromatin of fat body nuclei, a dnase i-hypersensitive site was found a ... | 1986 | 3941106 |
| purification of a cytotoxic protein produced by the murine macrophage-like cell line j774.1 in response to sarcophaga lectin. | a tumor specific cytotoxic protein produced by the murine macrophage-like cell line j774.1 in response to stimulation with sarcophaga lectin was purified to homogeneity in three steps from the culture medium. this cytotoxin, named tumor killing factor (tkf), was a protein with a molecular weight of 15,000, and aggregated forming an oligomer with a molecular weight of 48,000. its amino acid composition was similar to that of human tnf. purified tkf had a significant effect on transplanted murine ... | 1986 | 3957899 |
| homologies of nucleotide sequences in the 5'-end regions of two developmentally regulated genes of sarcophaga peregrina. | in the previous paper we demonstrated that the storage protein gene and 25-kda protein gene are expressed sequentially in the fat body of middle third-instar sarcophaga peregrina larvae. in this paper, we showed that the expressions of these two genes are regulated at the transcriptional level, and searched for homologous nucleotide sequences in the two genes in the vicinity of their 5'-ends, assuming that these two genes are regulated by a common mechanism. we selected 9 homologous sequences an ... | 1986 | 3960729 |
| electrical fields around the polytrophic ovarian follicles of sarcophaga bullata and the panoistic follicles of locusta migratoria. | 1986 | 3960906 | |
| identification of target proteins participating in a lectin-dependent macrophage-mediated cytotoxic reaction. | membrane proteins of mouse macrophages and mammary tumor cells having affinity to sarcophaga lectin were isolated by affinity chromatography. the electrophoretic profiles and antigenicities of lectin-binding proteins from macrophages and tumor cells were different. antibody raised against tumor cell lectin-binding proteins inhibited both the binding of the lectin to tumor cells and the lectin-dependent macrophage-mediated cytotoxic reaction. however, it did not inhibit the binding of the lectin ... | 1985 | 3965139 |
| participation of sarcophaga peregrina humoral lectin in the lysis of sheep red blood cells injected into the abdominal cavity of larvae. | sarcophaga lectin which is induced in the hemolymph of sarcophaga peregrina (flesh-fly) larvae when their body wall is injured with a hypodermic needle, was shown to participate in the lysis of sheep red blood cells introduced into the abdominal cavity of the larvae. this finding indicates that humoral lectin plays a role in the defence mechanism of invertebrates. the lysis of sheep red blood cells was enhanced greatly by preinjection of red blood cells, but less by preinjection of other cells. ... | 1985 | 3996706 |
| induction of selective phosphorylation of a fat body protein of sarcophaga peregrina larvae by 20-hydroxyecdysone. | 20-hydroxyecdysone was shown to induce selective phosphorylation of a fat body protein of sarcophaga peregrina larvae with a molecular mass of 30 kda. this phosphorylation was not associated with synthesis of new protein. fractionation of 32p-labelled fat body by differential centrifugation showed that this protein was mainly present in the membrane-rich fraction, although we could not specify the membrane. thus, 20-hydroxyecdysone may modify the function of the fat body by inducing phosphorylat ... | 1985 | 4004788 |
| comparison of binding proteins on the surface of murine tumor cells for two lectins active in the lectin-dependent macrophage-mediated cytotoxic reaction. | the binding proteins for sarcophaga lectin and wheat germ agglutinin on the surface of ehrlich ascites tumor cells were compared. studies with antibody against the binding protein for sarcophaga lectin showed that these two binding proteins are different. since these two lectins are both active in the lectin-dependent macrophage-mediated cytotoxic reaction with ehrlich ascites tumor cells as target cells, there must be multiple proteins on the surface of target cells that can trigger cytolytic r ... | 1985 | 4028023 |
| axonal projections from transplanted ectopic legs in an insect. | ectopic legs were produced in the fleshfly sarcophaga bullata by transplantation of leg imaginal discs. cobalt or horseradish peroxidase (hrp) fills from these supernumerary legs showed that their sensory axons invariably innervate the metathoracic leg neuropil via an abdominal nerve. this was so irrespective of whether they differentiated from pro-, meso-, or metathoracic leg disc. implantation of left leg discs on the right side and vice versa revealed that the site of implantation determined ... | 1985 | 4044939 |
| spermatid differentiation in the blowfly sarcophaga bullata with particular reference to flagellar morphogenesis. | 1971 | 4104241 | |
| a histochemical analysis of the proteins in the larval fat body of sarcophaga bullata. | 1966 | 4160236 | |
| variation of free amino acids in sarcophaga ruficornis during metamorphosis. | 1970 | 4248096 | |
| amino acid activating enzymes in sarcophaga bullata. | 1966 | 4287302 | |
| the deposition of lipid and the composition of neutral lipids in the fat body of sarcophaga bullata (diptera). | 1968 | 4296345 | |
| carbohydrate metabolism in flight muscle of the tsetse fly (glossina) and the blowfly (sarcophaga). | 1969 | 4312541 | |
| focal recording of responses evoked by light in the lamina ganglionaris of the fly sarcophaga bullata. | 1970 | 4319374 | |
| active ion transport in the larval hindgut of sarcophaga bullata (diptera: sarcophagidae). | 1974 | 4411835 | |
| co2-induced changes in the critical fusion frequency of the fly, sarcophaga bullata. | 1974 | 4412702 | |
| effect of ecdysterone contact period on development of wing disks of the fleshfly, sarcophaga peregrina, in vitro. | 1974 | 4413044 | |
| respiration-linked calcium ion uptake by flight muscle mitochondria from the blowfly sarcophaga bullata. | 1974 | 4415579 | |
| developmental cytology of the midgut in the flesh-fly, sarcophaga bullata (parker). | 1974 | 4432236 | |
| dna synthesis patterns in the giant foot-pad nuclei of sarcophaga bullata (sarcophagidae, diptera). | 1974 | 4442326 | |
| circadian control of larval growth rate in sarcophaga argyrostoma. | larvae of the flesh fly, sarcophaga argyrostoma, were reared in a range of environmental light cycles with periods from 21 to 72 hr, and containing either 12, 14, or 16 hr of white light. the mean length of larval development varied cyclically with the period of the driving light cycle. protracted development occurred when the larvae were "driven" by light cycles containing a "short day" (12 hr of light) at periods close to their natural circadian frequency (about 24 hr) or multiples thereof. mo ... | 1972 | 4506794 |
| [cause of life shortening in adult flesh fly, sarcophaga peregrina]. | 1973 | 4517303 | |
| a possible role of posterior half of larval body on the pupation of the fleshfly, sarcophaga peregrina. | 1972 | 4537700 | |
| induction and termination of pupal diapause in relation to the change of ecdysone titer in the fleshfly, sarcophaga peregrina. | 1972 | 4541840 | |
| relation of puffing to bristle and footpad differentiation in calliphora and sarcophaga. | 1972 | 4579363 |