Publications

agglutination of bordetella species by lectins.	phase i cells of bordetella pertussis but not those of b. parapertussis, b. bronchiseptica or b. avium were agglutinated by limulus polyphemus lectin. most strains of b. pertussis but not those of the other species were also agglutinated by helix pomatia lectin. in precipitation reactions between lectins and purified bordetella lipopolysaccharide (lps) preparations a similar pattern occurred. lectin agglutination provides a rapid presumptive method for the differentiation of b. pertussis from b. ...	1992	1555759
17 alpha,20 beta-dihydroxy-4-pregnen-3-one 20-sulphate: a major new metabolite of the teleost oocyte maturation-inducing steroid.	this paper describes the discovery of 17 alpha,20 beta-dihydroxy-4-pregnen-3-one 20-sulphate (17,20 beta-p-sulphate) in urine of male and female plaice pleuronectes platessa, in female atlantic salmon salmo salar and in female dover sole solea solea. 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-p) induces oocyte final maturation in teleosts and, whereas levels of the free steroid in maturing/ovulating female plaice are generally less than 1 ng ml-1 and poorly associated with the stage ...	2006	1563623
resistance of hamster bronchiolar epithelium to neutrophil elastase: investigation by cell surface lectin cytochemistry.	an intratracheal instillation of human neutrophil elastase (hne) causes accumulation of an excess number of secretory granules in the epithelial secretory cells lining the hamster bronchus. this chronic lesion, which we refer to as secretory cell metaplasia (scm), is not seen in the trachea or bronchioles. because luminal cell surface lectin binding is much higher in the trachea than in the bronchus, we concluded that tracheal resistance may be due to a protective glycoconjugate coat. in the pre ...	1992	1572319
carbohydrates in the cell surface of hair cells from the guinea pig cochlea.	the presence of cell surface carbohydrates was investigated in intact, non-fixed outer hair cells (ohcs) of guinea pigs using fluorescein isothiocyanate (fitc) and rhodamine (tritc) lectins. by means of wheat germ agglutinin (wga) n-acetyl-d-glucosamine was shown in the entire ohc membrane, including the stereocilia. binding of wga in ohcs to neuraminic acid was excluded by preincubation with neuraminidase. moreover, fitc-limulus polyphemus, a specific lectin for neuraminic acid, showed no fluor ...	1992	1581047
intravenous thyrotropin (tsh)-releasing hormone releases human tsh that is structurally different from basal tsh.	to determine whether basal tsh differed structurally from trh-released tsh, the tsh obtained from 11 normal subjects before and after the iv administration of trh was characterized using lectin-affinity chromatography. tsh was applied to the following lectins: lentil, ricin (both before and after tsh treatment with neuraminidase), concanavalin-a, wheat germ, glycine max, helix pomatia, dolichos biflorus, arachis hypogaea, and vicia villosa (isolectin b4). after each column was washed to elute un ...	1992	1592875
subdomains of the rough endoplasmic reticulum in colon goblet cells of the rat: lectin-cytochemical characterization.	using lectin binding, we characterized subdomains of the rough endoplasmic reticulum (rer) in goblet cells of the rat colon. in this cell type, special rer regions can be differentiated on the basis of their content of low electron density and dilated cisternal spaces in conventional transmission electron microscopic preparations. the fine fibrillar content of these cisternal regions demonstrated high-affinity binding with lectins from wheat germ, helix pomatia, griffonia simplicifolia i-a4 and ...	1992	1607641
the hamster transferrin receptor contains ser/thr-linked oligosaccharides: use of a lectin-resistant cho cell line to identify glycoproteins containing these linkages.	we recently reported that the human transferrin receptor (tfr) contains o-linked galnac residues [1]. to investigate whether this modification is shared by transferrin receptors in other mammals, we investigated the glycosylation of tfr in hamster cells. to facilitate our analysis the lectin-resistant chinese hamster ovary (cho) cell line lec8 was used. these cells are unable to galactosylate glycoproteins, resulting in truncation of the ser/thr-linked oligosaccharides to a single residue of ter ...	1992	1640050
phosphofructokinase from mollusc muscle is activated by phosphorylation.	phosphofructokinase was purified from muscle tissue of two different molluscs, edible snails, helix pomatia (gastropoda), and mussels, mytilus edulis (bivalvia). under denaturing conditions, both enzymes had a molecular mass of 82 kda. in the presence of atp-mg2+, the enzymes were rapidly phosphorylated in vitro by the catalytic subunit of cyclic amp (camp)-dependent protein kinase purified from snail muscle and also by the c subunit of protein kinase from bovine heart. the extent of phosphoryla ...	1991	1654810
the prognostic value of tumor-associated carbohydrate structures correlated with gene amplifications in human breast carcinomas.	the effects of cell surface sugar chains combined with certain gene amplifications of breast cancers on the prognosis of patients were studied and the relationships between the sugar chains of cancer cells and amplifications of the proto-oncogenes c-myc, int-2 and c-erb b-2, evaluated. one hundred and fifty three human breast carcinoma tissues were investigated by an immunohistochemical technique using the avidinbiotin-peroxidase method with 1 lectin (hpa; helix pomatia) and 4 monoclonal antibod ...	1991	1687600
lectin binding patterns to terminal sugars of rat lung alveolar epithelial cells.	we used post-embedding cytochemical techniques to investigate the lectin binding profiles of rat lung alveolar epithelial cells. sections from rat lung embedded in the hydrophilic resin lowicryl k4m were incubated either directly with a lectin-gold complex or with an unlabeled lectin followed by a specific glycoprotein-gold complex. the binding patterns of the five lectins used could be divided into three categories according to their reactivity with alveolar epithelial cells: (a) the limax flav ...	1990	1688898
polycystic disease of the kidney. evaluation and classification based on nephron segment and cell-type specific markers.	the usefulness of various segment and cell-type specific antibody, lectin and functional markers in the study of cystic renal lesions was evaluated. for this purpose, kidneys from recessive polycystic kidney disease (rpkd), thought to involve mainly the collecting ducts, and cystic kidneys of meckel's syndrome (ms), which show dilation randomly along the nephron, were studied. the segment (and differentiation-stage)-specific anti-brush-border (specific for proximal tubules) antibodies stained mo ...	1999	1690315
[determination of lectin receptors on the surface membranes of lymphoid cells].	the authors describe a method for the detection of carbohydrate determinants contained by the cellular surface membrane glycosylated biopolymers, based on the use of horse radish peroxidase-conjugated arachis, helix pomatia, ricinus communis, lens, glycine max, sophora japonica lectins. the pattern of lectin receptor distribution on normal human blood lymphocytes, tonsillar lymphoid cells, and in some forms of leukemia and lymphoma is shown. the authors consider it essential that a lectin kit be ...	1990	1692358
a plasma membrane antigen of rat glomerular epithelial cells. antigenic determinants involving n-linked sugar residues in a 140-kilodalton sialoglycoprotein of the podocytes.	the aim of the present study was to investigate the antigenic make up of the plasma membrane of rat glomerular visceral epithelial cells (gep). a crude plasma membrane fraction (pm) was extracted by 1% sodium dioxycholate from isolated rat glomeruli. pm was digested with neuraminidase (nrd) and purified by the helix pomatia agglutinin (hpa)-affinity column. when studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the hpa-affinity purified plasma membrane fraction (hpa-pm) forme ...	1990	1700199
changes in glycoconjugates revealed by lectin staining in the developing airways of syrian golden hamsters.	lectin binding was studied in the developing airways of syrian golden hamsters on gestational days 11-16 (day 16 is the day of birth). the trachea and lungs were fixed in 4% formaldehyde-1% glutaraldehyde, 6% mercuric chloride-1% sodium acetate-0.1% glutaraldehyde, and 95% ethanol; embedded in paraffin; and stained with eight lectin-horseradish peroxidase conjugates: triticum vulgare (wga), dolichos biflorus (dba), helix pomatia (hpa), maclura pomifera (mpa), griffonia simplicifolia i-b4 (gsa i- ...	1990	1700650
cell type-specific lectin staining of the tracheobronchial epithelium of the rat: quantitative studies with griffonia simplicifolia i isolectin b4.	we compared lectin staining patterns to cell population densities, as determined by morphological criteria in rat airways. eight lectins were studied: griffonia simplicifolia i isolectin b4 (gsi-b4), arachis hypogaea (pna), wisteria floribunda (wfa), glycine maximus (sba), dolichos biflorus (dba), helix pomatia (hpa), ulex europaeus (uea-1), and maclura pomifera (mpa). two of the lectins strongly stained morphologically distinct cell subpopulations. gsi-b4 stained basal cells, and mpa stained no ...	1991	1701188
difference in the ability of blood group-specific lectins and monoclonal antibodies to recognize the abh antigens in human tissues.	twelve different kinds of blood group-specific lectins have been used along with monoclonal anti-a, -b and -h antibodies for detecting the corresponding antigens in selected human tissues. although most of the lectins recognized the antigens in the tissue sections examined, they displayed marked differences in their recognition patterns in certain tissues. helix asparsa agglutinin (haa), helix pomatia agglutinin (hpa) and monoclonal anti-a antibody recognized a antigens in the mucous cells of sa ...	1990	1705925
[fine structure studies and identification of lectin receptors on the viral envelope of two hiv-isolates].	the electron microscopic particle findings were compared with the levels of revertase in corresponding samples over a longer period of time, and a good correlation was found. comparative investigations of the fine-structure of two hiv isolates did not reveal any morphological differences. it can be assumed, on the basis of the comparative studies on lectin receptors using helix pomatia lectin, that the viral envelopes of the two isolates are equipped similarly with n-acetyl-d-galactosamine. the ...	1990	1706878
experimental epilepsy in vitro: neuromodulating activity of anti-brain autoantibodies from rats exposed to electroconvulsive shock.	neuromodulating activity of anti-brain autoantibodies obtained from electroshocked (ecs) rats was tested on the neurons of isolated suboesophageal ganglion of the snail helix pomatia. in 16 out of 18 spontaneously active (pacemaker) neurons, ecs igg containing anti-brain autoantibodies induced short-lasting epileptiform discharges and membrane depolarization. membrane input resistance and time constant decreased, while membrane capacitance increased after addition of ecs igg. amplitude of evoked ...	1991	1723059
kaliotoxin, a novel peptidyl inhibitor of neuronal bk-type ca(2+)-activated k+ channels characterized from androctonus mauretanicus mauretanicus venom.	a peptidyl inhibitor of the high conductance ca(2+)-activated k+ channels (kca) has been purified to homogeneity from the venom of the scorpion androctonus mauretanicus mauretanicus. the peptide has been named kaliotoxin (ktx). it is a single 4-kda polypeptide chain. its complete amino acid sequence has been determined. ktx displays sequence homology with other scorpion-derived inhibitors of ca(2+)-activated or voltage-gated k+ channels: 44% homology with charybdotoxin (ctx), 52% with noxiustoxi ...	1992	1730708
presence of terminal n-acetylgalactosamine residues in subregions of the endoplasmic reticulum is influenced by cell differentiation in culture.	using helix pomatia lectin as a specific probe for terminal, nonreducing n-acetylgalactosamine residues, glycoprotein precursors bearing newly initiated o-linked oligosaccharides have been localized in the lumen of the endoplasmic reticulum and cis-golgi cisternae. this pattern contrasts with the detection of the terminal disaccharide galactose beta-1,3-n-acetylgalactosamine by arachis hypogaea lectin in middle and trans-golgi compartments, which are considered elongation sites for o-glycosylati ...	1991	1748669
carbohydrates in the guinea pig stria vascularis demonstrated with lectin-gold techniques.	soybean agglutinin (sba), helix pomatia agglutinin (hpa), ricinus communis agglutinin-ii (rca-ii), limax flavus agglutinin (lfa) and wheat germ agglutinin (wga) were employed to determine the localization of specific carbohydrates on thin sections of lowicryl k4m embedded guinea pig striae vasculares using the lectin-gold and glycoprotein-gold techniques. sba, hpa and rca-ii gold labeling was observed in many of the cytoplasmic vesicles, endosomes and apical tubules located in the supranuclear r ...	1991	1763624
use of thermospray liquid chromatography-mass spectrometry to aid in the identification of urinary metabolites of a novel antiepileptic drug, lamotrigine.	the use of thermospray liquid chromatography-mass spectrometry allowed the structural elucidation of a number of urinary metabolites of lamotrigine, 3,5-diamino-6-(2,3-dichlorophenyl)-1,2,4-triazine, formed after administering the drug to man, cynomolgus monkey and rabbit. this data when combined with the data obtained from high-performance liquid chromatography with radiochemical detection enabled us to determine the types and amounts of unchanged drug and metabolites excreted in urine by man a ...	1991	1795036
biochemical and serological properties of streptococcus uberis.	the strep-zym identification system, a combination of 23 enzymatic tests, allowed a rapid biochemical characterization of streptococcus uberis. the biochemical profiles of the s. uberis cultures clearly differed from those of s. agalactiae and s. dysgalactiae. serological grouping of s. uberis revealed polysaccharide antigens of groups e, g, p and u. some cultures of s. uberis demonstrated camp-like synergistic hemolytic activities on sheep blood agar and reacted specifically with the lectins of ...	1991	1796686
[how thick is the glycocalyx of human erythrocytes?].	the aim of the experiment was to determine the thickness of the glycocalyx of human erythrocytes of the blood group a. for that purpose, the distance between the electron dense contrasted lipid layer of the plasmalemma and gold sol particles loaded with helix pomatia lectin was determined. the mean thickness of the glycocalyx under this conditions was 5.9 nm.	1991	1801510
defining the specificity of anti-a/b igm/g antibodies with different antigens and lectins.	the specificity of anti-histo blood group a/b antibodies is defined by the antigen used for their production and selection (in the case of mouse monoclonal antibodies, mabs) or by the antigens present on various intestinal bacteria (for polyclonal human igm and/or igg, phabs). absorption experiments with red blood cells and free antigen have been used earlier to define specificity; here we use a/b substances of human, animal and synthetic origin as well as known reactivities of mabs or lectins a ...	1991	1862641
a 58-kda resident protein of the cis golgi cisterna is not terminally glycosylated.	a 58-kda golgi protein (gp58) was previously identified and found to be concentrated in cis golgi cisternae in several cell types (saraste, j., palade, g.e., and farquhar, m.g. (1987) j. cell biol. 105, 2021-2029). in this study the protein was partially purified from rat pancreas and mouse myeloma cells in order to characterize its oligosaccharides. it migrated on sodium dodecyl sulfate-polyacrylamide gels as a 57-58-kda doublet under reducing conditions or as a single approximately 116-kda ban ...	1991	1894639
biosynthesis in vitro of glca beta 1-3nlcose4cer by a novel glucuronyltransferase (glcat-1) from embryonic chicken brain.	a novel glucuronyltransferase (glcat-1) has been detected in embryonic chicken brains. this enzyme catalyzes the biosynthesis in vitro of glucuronic acid containing glycolipids starting from neolactotetraosylceramide (nlcose4cer) and neolactohexaosylceramide (nlcose6cer). the activity is present primarily in the golgi-rich membrane fraction and can be extracted (60%) from the membrane using a neutral detergent, nonidet p-40, at ph 7.0. the detergent-solubilized glcat-1 is stable (70%) at -20 deg ...	1991	1900517
glycoconjugates in the human fetal endolymphatic sac as detected by lectins.	the distribution of glycoconjugates in the 11 to 16 weeks old human fetal endolymphatic sac (es) was analyzed using six biotinylated lectins; wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). in the 11 week old human fetus, fluorescent reactions with wga, apa, rca120 and cona were detected in the es. there was almost no reaction with hpa and uea-i. ...	1991	1919336
species identification and characterization of an acanthamoeba strain from human cornea.	the isoenzyme pattern of an acanthamoeba, stock h-1, isolated from a patient with keratitis (krankenhaus heidberg, hamburg) was compared with that of two strains of a. quina-a. lugdunensis (302-2, 312-1), two stocks of a. lenticulata (45, 89-1) and one strain of a. rhysodes (302-1). the isolated stock showed glucose-6-phosphate dehydrogenase (g-6-pdh), beta-hydroxybutyric dehydrogenase (beta-hbdh), alcohol dehydrogenase (adh) and superoxide dismutase (sod) isoenzyme patterns similar to those of ...	1991	1924251
decreased biosynthesis of forssman glycolipid after retinoic acid-induced differentiation of mouse f9 teratocarcinoma cells. lectin-affinity chromatography of the glycolipid-derived oligosaccharide.	glycolipids synthesized by the mouse teratocarcinoma f9 cells and f9 cells (ra/f9 cells) induced to differentiate by a 3-day treatment with 0.1 microm all-trans-retinoic acid were analyzed. both f9 cells and ra/f9 cells were incubated in media containing either d-[6-3h]galactose or d-[6-3h]glucosamine; the metabolically-radiolabeled glycolipids were isolated and the oligosaccharides were released from the glycolipids by ozonolysis and alkali fragmentation. from both cells, a single major pentasa ...	1991	1933935
metabolism and fate of ecdysteroids in the nematodes ascaris suum and parascaris equorum.	when injected with [3h]ecdysone and maintained in vitro, the parasitic nematodes, ascaris suum and parascaris equorum each produced a series of polar and relatively apolar metabolites. a. suum metabolised the compound into ecdysonoic acid ([3h]eoic), ecdysone 25-glucoside ([3h]e25gluc), putative ecdysone 22-phosphate ([3h]e22p) and a series of at least six relatively apolar metabolites. all of these, except ecdysonoic acid, were hydrolysed by a crude enzyme preparation from helix pomatia, releas ...	1991	1944416
aluminofluorides and beryllofluorides as inhibitors of sulphatases. analogues of hydrogen sulphate?	the inhibition of fluoride of sulphatase a from ox liver and of the sulphatases of helix pomatia and aspergillus oryzae is decreased by edta and increased by al3+ or be2+, implicating aluminofluorides and beryllofluorides in the reaction. the inhibition, which is reversible, takes several minutes to develop fully and, at least for the sulphatase of h. pomatia, is of a non-linear mixed competitive-non-competitive type. it is suggested that the aluminofluorides and beryllofluorides are acting as a ...	1991	1953634
the adsorption of bacteria to immobilized lectins.	the agglutination of a selection of bacteria by some lectins was examined. the lectin from codium fragile agglutinated seven strains of salmonella typhimurium. the lectin from helix pomatia agglutinated eight of 12 strains of listeria monocytogenes and a further two strains gave a weak agglutination reaction. helix pomatia lectin conjugated to magnetic microspheres enabled the adsorption of l. monocytogenes from suspension with subsequent elution by the competing ligand n-acetyl galactosamine. a ...	1991	1955420
neurohypophysial peptides selectively depressed high voltage-activated ca-current in snail neurons.	it was found that neurohypophysial peptides oxytocin and lys-vasopressin selectively decreased the high voltage-activated component of the inward ca-current in voltage-clamped helix pomatia identified neurons in a dose- and time-dependent manner. this effect was unaffected or further enhanced applying phosphodiesterases inhibitors. it is suggested that suppression of high voltage-activated current was due to the activation of the adenylate cyclase system, likely protein kinase a.	2007	1963481
expression of blood group-related antigens and helix pomatia agglutinin in malignant pleural mesothelioma and pulmonary adenocarcinoma.	in an attempt to differentiate malignant pleural mesothelioma from pulmonary adenocarcinoma by histochemical and immunohistochemical means, the glycoconjugate profiles of five reactive mesothelial lesions, 29 mesotheliomas (20 epithelial, three biphasic, and six fibrous types), and 38 well-differentiated pulmonary adenocarcinomas (34 papillary, two tubular, and two bronchioloalveolar types) were tested with abh blood group-related antigens (bgr-ag) antibody and helix pomatia agglutinin (hpa) whi ...	1991	2001875
binding of platelet agglutinating protein p37 from the plasma of a patient with thrombotic thrombocytopenic purpura to human platelets.	we have previously reported the purification of a 37 kda platelet agglutinating protein (pap p37) from the plasma of a patient with thrombotic thrombocytopenic purpura (ttp) that was shown to be present in a subset of ttp patients. to gain further insight into the interaction between pap p37 and platelets, we have studied the properties of pap p37 binding to platelets. washed human platelets from two normal donors and two ttp patients after recovery were used for the experiments. the pap p37 bin ...	1991	2024244
lectin histochemistry of developing submandibular glands of fetal syrian golden hamsters.	lectin binding was studied in the developing submandibular glands of fetal syrian golden hamsters (mesocricetus auratus) from gestational day 12 to 16 (the day of birth). the fetuses were fixed, embedded in paraffin, sectioned and stained with nine lectin-horseradish peroxidase conjugates: concanavalin a (con a), wheat germ agglutinin (wga), dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), maclura pomifera agglutinin (mpa). griffonia simplicifolia agglutinin i-b4 (gsa i-b4), p ...	1991	2035849
glycoconjugate distribution in the human fundic mucosa revealed by lectin- and glycoprotein-gold cytochemistry.	the glycoconjugates of the human fundic mucosa were characterized at the ultrastructural level by means of direct (helix pomatia agglutinin-gold complex) and indirect lectin techniques (concanavalin a and horseradish peroxidase-gold complex; wheat germ agglutinin and ovomucoid-gold complex). surface mucous cells and mucous neck cells secreted o-glycoproteins with n-acetylgalactosamine and n-acetylglucosamine residues at the non reducing terminus of the saccharidic chain. the secretory granules o ...	1990	2081692
characterization of galactose-containing glycoconjugates in the human retina: a lectin histochemical study.	seven specimens of morphologically normal formalin-fixed and paraffin-embedded human retina were studied using a panel of fourteen biotinylated lectins, all of which react with glycoconjugates containing galactose and n-acetylgalactosamine residues. agaricus bisporus (aba), bauhinia purpurea (bpa), phaseolus vulgaris (pha-e), peanut (pna) and ricinus communis (rca-i) agglutinins labeled photoreceptor cells prior to enzymatic predigestion. bpa and pna bound specifically to cones. the plexiform la ...	1990	2091899
use of peldri ii as a sublimation dehydrant in place of critical-point drying in fracture-label cytochemistry and in backscattered electron imaging fracture-label.	peldri ii, a fluorocarbon compound solid at room temperature, was used as a sublimation dehydrant in place of critical-point drying (cpd) in freeze-fracture cytochemistry. its applicability to the fracture-label replica method was demonstrated using freeze-fractured rat pancreas labeled with helix pomatia lectin-gold complex and phospholipase a2-gold complex for the detection of glycoconjugates and phospholipids, respectively. no difference in morphology and labeling pattern was detected in cpd- ...	1990	2153786
interaction of bluetongue virus with bovine lymphocytes.	freshly isolated, and established, cultures of bovine peripheral blood mononuclear leukocytes (pbmls) were exposed to bluetongue virus (btv) for the purpose of defining potential lymphotropism. pbml cultures were established in the presence of interleukin 2 (il-2) and mitogen and maintained either as bulk culture or were cloned prior to infectivity studies. all cultures appeared to be of the t cell phenotype based on the following characteristics: binding of t lymphocyte-specific lectins (i.e. p ...	1990	2155289
flow cytometric analysis of cell surface carbohydrates in metastatic human breast cancer.	helix pomatia agglutinin (hpa)- and concanavalin a (con a)-binding carbohydrate expression were studied on 32 tumour samples from primary adenocarcinoma of the breast and 12 samples from lymph node metastases. live cells were spilled from each of the fresh samples and the extent of fluorescent-labelled hpa and con a-binding was assessed by flow cytometry. the extent of brightness was expressed in a defined quantitative fashion and the percentage of positive cells was accurately determined from a ...	1990	2167120
[a neurophysiological analysis of the response pattern to direct electrical stimulation of the lpa3 and rpa3 neurons in the edible snail].		2007	2169170
characterization of the high mr glycoprotein (gp300) of equine herpesvirus type 1 as a novel glycoprotein with extensive o-linked carbohydrate.	the high mr glycoprotein (gp300) of equine herpesvirus type 1 was found to have an mr, estimated by sds-page, of over 400,000 and was confirmed as being a surface glycoprotein by 125i-labelling. in contrast to [3h]glucosamine, gp300 showed very low levels of [3h]glucosamine, gp300 showed very low levels of [3h]mannose incorporation. the mr of gp300 showed no detectable change upon treatment of purified virus with n-glycanase, and showed only a small change in virus-infected cells treated with tu ...	1990	2172454
time courses of lidocaine effects on sodium membrane currents in small and large neurons.	time courses of effects of lidocaine on sodium currents and sodium dependent action potentials were studied in somata of small and large neurons. cultured rat sensory spinal ganglion cells (diameter: 30 microns) and neurons of the buccal ganglion of helix pomatia (diameter: 150 microns) served as the test cells. the latency of the suppressive action of lidocaine was the longer the larger the of the cells was. maximal blocking effects occurred within 10 min in sensory spinal ganglion cells and wi ...	1990	2177020
effects of alcohol on lectin binding affinity in rat gastric mucosa.	fluoresceinated lectins were employed to qualitatively evaluate cell surface carbohydrates, with and without ethanol exposure, in rat stomach mucosae. rats received 1 ml of saline, or 50% or 100% ethanol orally. after 30 min, tissue samples of the glandular stomach were retrieved, cryosectioned, and incubated with one of a panel of lectins. another set of sections was preincubated with neuraminidase to remove sialic acid residues. qualitative evaluation of lectin binding showed that although sev ...	1990	2194768
transient expression of type 2 chain in a-active hexaglycosylceramide of rat small intestine at weaning time. demonstration by affinity chromatography and ceramide glycanase hydrolysis of a-active glycosphingolipids followed by gas chromatography and mass spectrometry of permethylated hexasaccharides.	the small intestine of 15- to 23-day-old rats was cut into four segments from the duodenum to the ileum. neutral glycosphingolipids were purified from each segment and submitted to thin-layer chromatography and immunostaining with the a005 monoclonal anti-a antibody. this antibody detected an hexaglycosylceramide located mainly in the duodenum during the postnatal development. in order to characterize hexaglycosylceramides, blood group a-active glycolipids were purified by affinity chromatograph ...	1990	2221915
helix pomatia agglutinin binds specifically to the golgi apparatus in cultured human fibroblasts and reveals two golgi apparatus-specific glycoproteins.	fluorochrome-coupled helix pomatia agglutinin (hpa), but not other lectin-conjugates with the same nominal specificity, bound specifically to the golgi apparatus in cultured human fibroblasts, revealing a cytoplasmic juxtanuclear reticular structure. unlike other golgi-binding lectins the hpa-conjugates did not bind to the cell surface membrane or pericellular matrix. experiments with 35s-methionine-labeled cells showed that hpa recognized two glycoproteins of mr 170,000 and 400,000 among the se ...	1990	2228736
the reaction of nitrite with the haemocyanin of the roman snail (helix pomatia).	the reaction of nitrite at ph 5.0-7.0 with the deoxyhaemocyanin of a mollusc, the roman snail (helix pomatia), yielded nitrosylhaemocyanin (cuia.no+ cuiib), in contrast with the formation of methaemocyanin with the deoxyhaemocyanin of the crustacean astacus leptodactylus (mud crayfish). with helix haemocyanin 1 no was thereby liberated per active site, as shown by m.s., as against 2 no with astacus haemocyanin. helix nitrosylhaemocyanin was characterized in c.d. by the negative extremum at 336 n ...	1990	2244878
[study of lymphocyte subpopulations using peanut, soybean and snail lectins].	the method of penning in plastic dishes was to isolate the pna+ and pna- cell populations from the tonsil tissue. carbohydrate determinants of lymphoid cell surface membranes being at varying stages of antigen-independent and antigen-dependent differentiation have been studied with the use of peanut, soya and edible snail lectins conjugated with horse radish peroxidase.	1990	2253858
[monoclonal antibodies to individual antigens of the nervous system of the snail, helix pomatia].	seven positive hybridoma clones were chosen by immunoenzyme analysis amons 103 clones obtained by hybridization of nso plasmocytoma cells and splenocytes from balb/c mice, immunized with snail's nervous system antigens. specific binding of mabs with neuron cytoplasmic antigens was indicated on cryostat sections of visceral, pedal and cerebral ganglia. the mabs obtained could be used for the study of physiological role of antigens identified.	2008	2268718
heterogeneity of the blood group abh antigens and variation in the expression of these antigens of secretory granules in human cervical glands. an electron microscopic observation using lectins and monoclonal antibodies.	cytochemical localization of blood group abh antigens was examined in secretory cells of human cervical glands by application of a post-embedding lectin-gold as well as immuno-gold labeling procedure using monoclonal antibodies. blood group specific lectins such as dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), griffonia simplicifolia agglutinin i-b4 (gsai-b4) and ulex europaeus agglutinin-i (uea-i) reacted with secretory granules but not with other cytoplasmic organellae su ...	1990	2283312
binding of the blood group-reactive lectins to human adult kidney specimens.	the binding of a panel of blood group-reactive lectins to frozen sections of human kidney was studied with a special emphasis on reactivity with endothelia and basement membranes. the blood group a-reactive lectins, all specific for alpha-d-n-acetylgalactosamine (galnac), helix aspersa (haa), helix pomatia (hpa), and griffonia simplicifolia i-a4 (gsa-i-a4) agglutinins bound to the endothelium in specimens with blood groups a and ab. in other samples, these lectins reacted predominantly with tubu ...	1990	2297076
ultrastructural comparative distribution of carbohydrates in human tracheal and frog palate mucosa using neuraminidase and lectin-colloidal gold complexes.	we have compared, at the ultrastructural level, the carbohydrate structure of glycoconjugates of the different types of secretory cells of the human tracheal mucosa (htm) and the frog palate mucosa (fpm), proposed as a model for studying bacterial adherence to mucus-coated respiratory epithelium. in addition to reactivity with concanavalin a and lens cullinaris agglutinin, reactivity of epon-embedded htm and fpm secretory granules was studied by transmission electron microscopy using neuraminida ...	1990	2311103
identification of glucose conjugates as major urinary metabolites of cannabidiol in the dog.	1. three dogs were treated i.v. with cannabidiol (cbd) and urine collected at intervals to 30 h. 2. metabolites were extracted, converted into trimethylsilyl (tms) derivatives and examined by g.l.c.-mass spectrometry. 3. the major metabolites excreted at early times were identified as the phenol glucosides of 4"-hydroxy-cbd, 5"-hydroxy-cbd and 6-oxo-cbd. 4. these three oxidized metabolites were not found unconjugated, and none of the free oxidized metabolites in urine were found conjugated with ...	1990	2333714
ultracytochemistry of glycoconjugates in rat brunner's gland with labeled lectins.	mucous glycoproteins of rat brunner's gland were examined ultracytochemically to elucidate the intracellular localization and sites of glycosylation in relation to the functional polarity of the cell organellae using a combination of hydrophilic resin embedment and postembedding staining with labeled lectins. a few cis cisternae were stained with hpa (helix pomatia agglutinin, specific for terminal alpha-n-acetylgalactosamine) and dba (dolichos biflorus aggulutinin, specific for terminal alpha-n ...	1990	2358769
lectin cytochemistry reveals differences between hamster trachea and bronchus in the composition of epithelial surface glycoconjugates and in the response of secretory cells to neutrophil elastase.	hamsters exposed to an intratracheal instillation of human neutrophil elastase (hne) accumulate an abnormally high number of secretory granules in bronchial but not tracheal epithelial cells. we employed lectin cytochemistry to investigate possible differences in the epithelial cell surface glycoconjugate layer in trachea compared to bronchus which might explain the regional dissimilarity in response to hne. portions of glutaraldehyde-fixed trachea and bronchi were incubated in one of several fe ...	1990	2363936
comparison of the carbohydrate-binding specificities of seven n-acetyl-d-galactosamine-recognizing lectins.	seven plant lectins, dolichos biflorus agglutinin (dba), griffonia simplicifolia agglutinin (gsa, isolectin a4), helix pomatia agglutinin (hpa), soybean (glycine max) agglutinin (sba), salvia sclarea agglutinin (ssa), vicia villosa agglutinin (vva, isolectin b4) and wistaria floribunda agglutinin (wfa), known to be specific for n-acetyl-d-galactosamine-(galnac) bearing glycoconjugates, have been compared by the binding of their radiolabelled derivatives, to eight well-characterized synthetic oli ...	2008	2384093
surface array proteins of campylobacter fetus block lectin-mediated binding to type a lipopolysaccharide.	campylobacter fetus strains with type a lipopolysaccharide (lps) and a surface array protein layer (s+) have been found to be pathogenic in humans and animals. spontaneous laboratory mutants that lack surface array proteins (s-) are sensitive to the bactericidal activity of normal human serum. the ability of lectins to determine the presence of the s-layer and differentiate lps type was assessed. we screened 14 lectins and found 3 (wheat germ agglutinin, bandeiraea simplicifolia ii, and helix po ...	1990	2387622
characterization of candida antigens by crossed-immunoaffinoelectrophoresis.	the antigens of three candida albicans strains (3153 a, 3156 b and cbs 1905) and one c. tropicalis strain were studied by means of crossed-immunoaffinoelectrophoresis with the corresponding polyvalent antisera. most antigens (from 63.8% to 77.7% depending on the strain) were bound to concanavalin a-sepharose and about 20% to blue cibacron-sepharose for all the strains tested. free concanavalin a, wheat germ lectin-sepharose and helix pomatia lectin-sepharose revealed differences between c. albic ...	1985	2409611
alterations in glomerular lectin binding sites of human kidney as detected by fluorescence microscopy.	different lectins were used to study frozen sections of kidney samples showing alterations in routine immunofluorescence studies. arachis hypogaea agglutinin (peanut lectin, pna), lacking binding sites in normal glomeruli, bound to the glomeruli in two of the five samples studied, giving a granular fluorescence pattern. concomitantly with the appearance of pna-binding, binding sites for wheat germ agglutinin (wga) appeared to be lost at glomeruli. furthermore, changes in the expression of glomer ...	1985	2415488
lyophilization of protein-gold complexes.	conditions for dialysis, freezing and lyophilization of protein-gold complexes were established. lyophilized complexes formed by colloidal gold with staphylococcal protein a, with helix pomatia lectin and with horseradish peroxidase retained their original staining properties when reconstituted after several months of storage.	1985	2416715
tn epitopes, immunoreactive with ordinary anti-tn antibodies, on normal, desialylated human erythrocytes and on thomsen-friedenreich antigen isolated therefrom.	hybridoma generation, using specifically, maximally desialylated human blood group o erythrocytes (t rbc) as immunogen, and biochemical studies suggested the presence of immunogenic tn epitopes. galnac alpha-o, on t rbc. we therefore investigated by immunochemical means whether or not tn-specific epitopes immunoreactive with anti-tn antibodies present in ordinary human sera occur on t rbc and on thomsen-friedenreich (t) antigen prepared from them. we did detect the tn epitope with such antibodie ...	1985	2417112
regional heterogeneity of glycoconjugate distribution in the glomerulus revealed by lectin-gold cytochemistry and sds-page.	the authors have used sds-page and lectin overlay analysis in parallel with lectin-gold cytochemistry to identify helix pomatia lectin (hpl) binding glycoconjugates in rat kidney glomeruli. previous work revealed hpl binding sites only beneath podocyte foot process bases, where they contact the glomerular basement membrane. it is shown here that after neuraminidase digestion of thin sections of glomeruli before incubation with hpl-gold complexes, the number of hpl binding sites is markedly incre ...	1986	2432793
cytochemical characterization of mouse l1210 leukemia.	mouse lymphatic leukemia l1210 cells were characterized with polyclonal and monoclonal antibodies and lectins. the cells were found to have the phenotype igg-, thy-1.2-, lyt-1-, lyt-2-, asialo-gm1-, tl-, i-ad-, il-2r+, peanut agglutinin+, and helix pomatia lectin +/-. they retained expression of h-2kd and h-2dd. thus, these cells resemble "null" cells.	1988	2466781
tn epitope (n-acetyl-d-galactosamine alpha-o-serine/threonine) density in primary breast carcinoma: a functional predictor of aggressiveness.	this interpretive review attempts to dovetail advanced work by different groups of investigators on blood group and carcinoma (ca) glycoconjugates that have terminal, immunoreactive tn epitopes (galnac alpha-o-ser/thr), and on the interaction of those structures with complementary antibodies and lectins. fenlon et al. (1987) and leathem and brooks (1987) found a positive correlation between primary breast ca aggressiveness and its affinity for helix pomatia (hpa) lectin. this phenomenon was used ...	1989	2467192
lectin-binding sites in squamous cell carcinomas and keratoacanthomas.	the lectin-binding pattern of malignant and benign human epidermal tumours was studied. twelve squamous cell carcinomas and ten kerato-acanthomas were investigated, using fluorochrome-conjugated lectins. lectins used were canavalia ensiformis agglutinin (con-a), helix pomatia agglutinin (hpa), phaseolus vulgaris agglutinin (pha-l), and ricinus communis agglutinin (rca-120). two types of fluorescence pattern, intercellular 'pemphigus-like' and peritumoral, were noted. both allowed classification ...	1988	2467485
saccharide residues in human gingiva as revealed with fluorochrome-coupled lectins.	the histochemical binding of 16 fluorochrome-conjugated lectins to human marginal gingiva was investigated. of a total of 14 galactose/n-acetylgalactosamine (gal/galnac)-specific lectins, dolichos biflorus (dba), helix pomatia (hpa), and helix aspersa agglutinins (haa) were blood group a-reactive whereas griffonia simplicifolia i-b4 (gsa-i-b4) and sophora japonica (sja) agglutinins were blood group b-reactive. hpa, haa and gsa-i-b4 bound to all suprabasal epithelial cells and to vascular endothe ...	1989	2542513
rapid detection of herpes simplex virus with fluorescein-labeled helix pomatia lectin.	the use of fluorescein-conjugated helix pomatia lectin was shown to be as effective as fluorescein-conjugated monoclonal antibody reagents for the detection and differentiation of herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) in mrc-5 cell culture. cells infected with hsv-1 generally displayed a pattern of nongranular or diffuse fluorescence, while cells infected with hsv-2 were identified by the production of fluorescent grains and flecks. this unique nonimmunological reagent, when u ...	1989	2545739
[the effect of calcium ions on the deactivation of the calcium current in nerve cells of helix pomatia].		2008	2546725
cytochemical demonstration of lectin binding-sites in the cuticle and tissues of acanthocheilonema viteae (filarioidea).	the lectin-gold technique was used for the ultrastructural localization of lectin binding sites on thin sections of lowicryl k4m embedded adult females, infective larvae and sds-2-mercaptoethanol-insoluble cuticle components of acanthocheilonema (dipetalonema) viteae. helix pomatia lectin (hpl) coupled to 14 nm gold particles, was used for the demonstration of n-acetyl-d-galactosamine-containing glycoconjugates. triticum vulgaris (wheat germ) agglutinin (wga) coupled to 10 nm gold particles afte ...	1989	2566259
isolation and identification of 6-desmethylnaproxen sulfate as a new metabolite of naproxen in human plasma.	a new metabolite of naproxen, 6-desmethylnaproxen sulfate (6-dmns), has been identified in plasma from normal and uremic subjects after oral administration of a single 500 mg dose of naproxen. tentative identification was achieved by the finding of an increase in the concentration of 6-dmn upon incubation of the plasma with arylsulfatase from helix pomatia or from aerobacter aerogenes. more definitive identification was established through demonstration that the hplc retention time of the conjug ...	1989	2566468
cytoplasmic intermediate filament proteins of invertebrates are closer to nuclear lamins than are vertebrate intermediate filament proteins; sequence characterization of two muscle proteins of a nematode.	the giant body muscle cells of the nematode ascaris lumbricoides show a complex three dimensional array of intermediate filaments (ifs). they contain two proteins, a (71 kd) and b (63 kd), which we now show are able to form homopolymeric filaments in vitro. the complete amino acid sequence of b and 80% of a have been determined. a and b are two homologous proteins with a 55% sequence identity over the rod and tail domains. sequence comparisons with the only other invertebrate if protein currentl ...	1989	2583097
[the effect of high energy irradiation on the membrane potential and impulse activity of neurons in the brain of helix pomatia].	in electrophysiological experiments with a preparation of the isolated helix pomatia brain, a study was made of the effect of pulsed irradiation with high-energy electrons (20 mev) on membrane potentials and pulse activity of "silent", pacemaking and postsynaptic neurons. it was shown that after irradiation with 150 and 300 gy (dose rate 5 gy/s and pulse frequency 50 hz) "silent" neurons retain their excitability. pacemaking neurons responded to radiation by a drastic increase in spontaneous pul ...	2008	2587699
a new method for hydrolyzing sulfate and glucuronyl conjugates of steroids.	a new method for hydrolyzing steroid conjugates (both sulfates and glucuronides conjugates) that is efficient, effective, and inexpensive is described. this method comprises incubation of the conjugates--after salting-out into ethyl acetate or elution from a c18 cartridge--with anhydrous methanolic hydrogen chloride (methanolysis) for 10 min. it has been successfully applied to our routine radioimmunoassay screening and gc/ms confirmation studies of steroids in prerace and postrace equine urine ...	1989	2610346
physicochemical properties of a human glycoprotein bearing blood group a activity.	a human erythrocyte glycoprotein was isolated and purified from blood of group a+1 by a procedure involving chloroform-methanol extraction and affinity chromatography on helix pomatia lectin-sepharose 6mb, and some of its physicochemical properties were determined. the resulting preparation was homogeneous as indicated by polyacrylamide gel electrophoresis. the glycoprotein contained nearly 60% carbohydrate and 40% protein. it was water-soluble and inhibited the agglutination of a-erythrocytes. ...	1989	2613683
reactions of the lectin anti-ahp from the edible snail helix pomatia with n-acetyl-d-galactosamine of streptococci.	the lectin anti-ahp obtained from the albumen gland of the edible snail, helix pomatia, was used for the routine identification of group c streptococci. without any exception all 1,045 strains of group c streptococci (s.equisimilis, and s.zooepidemicus, and s.dysgalactiae) were agglutinated by the helix lectin, but none of the 12,264 strains of group a, 1,346 strains of group g, 330 strains of group b as well as that of other streptococcal groups. the presence of type-specific t antigens of grou ...	1989	2622116
topographical and planar distribution of helix pomatia lectin-binding glycoconjugates in secretory granules and plasma membrane of pancreatic exocrine acinar cells of the rat: demonstration of membrane heterogeneity.	the lectin-gold technique was used to detect helix pomatia lectin (hpl) binding sites directly on thin sections of rat pancreas embedded in lowicryl k4m and on freeze-fractured preparations of rat pancreas submitted to fracture label. on thin sections of acinar cells, whereas the content of zymogen granules was negative or weakly labeled, the limiting membrane displayed a high degree of labeling. in the golgi complex, labeling by hpl was localized on the trans saccules and the limiting membrane ...	1989	2672769
experimental glomerulonephritis induced in rats by a lectin and its antibodies.	the aim of the present study was to investigate the events following interaction of antibody with an antigen planted on the surface of glomerular endothelial cell (gen). a lectin, helix pomatia agglutinin (hpa), was planted at the surface of rat gen by the perfusion of the isolated left kidney with neuraminidase (nrd) and hpa. a subsequent perfusion with igg fraction, but not with fab fragments, of rabbit anti-hpa serum induced formation of granular immune deposits (ids) on the surface of gen. w ...	1989	2689748
mitogenic lectins bind to the antigen receptor on human lymphocytes.	the specificity of interactions between mitogenic and non-mitogenic lectins and disulfide-linked cell surface receptors on human lymphocytes was explored. lysates (nonidet-p40) of surface-radioiodinated tonsil lymphocytes and t lymphoblastoid cells (hpb-all) were absorbed with lectin-agarose derivatives (or bovine serum albumin, bsa-agarose) or immunoprecipitated with appropriate monoclonal antibodies (mab). lectin eluates and solubilized immunoprecipitates were analyzed by two-dimensional (nonr ...	1989	2703017
lectin-gold cytochemistry of the golgi apparatus in rabbit luteal cells, with special emphasis on the formation of a lysosomal-type membrane.	in rabbit luteal cells embedded in glycolmethacrylate and stained with pta at low ph highly glycosylated membrane patches can be observed after vesiculation of the trans-golgi network. as these membranes could be prelysosomal, their sialic acid content was investigated by post-embedding labeling with limax flavus agglutinin (lfa)/fetuin-au. additional labeling of the golgi apparatus was performed with wheat germ agglutinin (wga)/ovomucoid au, ricinus communis agglutinini (rcai)/au and helix poma ...	1989	2715051
purification of cadmium-binding proteins from related species of terrestrial helicidae (gastropoda, mollusca): a comparative study.	three species of terrestrial helicidae (helix pomatia, cepaea hortensis and arianta arbustorum) were fed cadmium-rich diet in the laboratory. the snails accumulated high amounts of the metal in their hepatopancreas. most cadmium and some zinc were found, after centrifugation, in the soluble fractions from which a cadmium-binding protein was isolated for each species by ion exchange and gel chromatography. the proteins contained different amounts of cadmium, but little or no zinc, and showed high ...	2009	2725484
[experimental infection of 2 species of laboratory rodents with invasive larvae of elaphostrongylus cervi (nematoda, metastrongyloidea)].	single doses (from 300 to 1000 larvi per an animal) of invasive larvae e. cervi cameron, 1931, obtained from experimentally infected snails helix pomatia l. were given to 17 guinea pigs and 17 golden hamsters. clinical nervous symptoms in the form of paresis and paralysis of limbs occurred only in the guinea pigs which were given a dose of 1000 larvi. these animals died in the period from the 75th to 117th day of infection. from their central nervous system single adult males and females of e. c ...	1989	2728450
demonstration of sugar residues in the ultimobranchial tubule and thyroid c-cells of the rat using peroxidase labelled lectins.	the distribution of various sugars, that contribute to the composition of the carbohydrate components of the glycoprotein in the ultimobranchial tubule and the thyroid c-cells has been studied, using eight peroxidase-labelled lectins (dolichos biblorus, glycine max, bauhinia purpurea, helix pomatia, arachis hipogaea, ulex europaeus i, limax flavus and sophora japonica). the results obtained seem to show that there are receptors for dolichos biflorus, glycine max, bauhinia purpurea, helix pomatia ...	1989	2757235
reactivity of visceral yolk sac endoderm with lectins: changes related to age and species.	the expression of receptors for lectins reacting with extraembryonal endoderm was compared between mouse and rat at different stages of gestation. fluorescein-conjugated helix pomatia agglutinin (hpa), dolichos biflorus agglutinin (dba), sophora japonica agglutinin (sja), peanut agglutinin (pna), bandeiraea simplicifolia agglutinin 1 (bsa-1) and gold-conjugated dba and pna were used. it was shown that the rat visceral endoderm does not express the receptors for hpa, while the mouse tissue does. ...	1989	2762737
synthesis and initial processing of gastric apomucin.	the initiation of the processing of apomucin was investigated using mucus glycoprotein synthesizing polysomes from rat gastric epithelial cells. the polysomes were isolated from cells labeled with [3h]palmitic acid and [14c]n-acetylgalactosamine, purified on helix pomatia-sepharose affinity column, dissociated to release peptidyl-trna, and chromatographed on deae-hplc column to separate peptidyl-trna complexes from the free and ribosomal rna and proteins. the analysis of the hplc purified peptid ...	1989	2764978
lectin histochemistry in ulcerative colitis and crohn's disease.	the glycoconjugate composition of intestinal goblet cell mucin was characterized according to the anatomical distribution of lectin-binding sites in surgically resected intestinal tissues and mucosal biopsy specimens obtained from 38 control subjects, and from 32 patients with the active phase of ulcerative colitis, and 12 with crohn's disease. immunoperoxidase labeling studies found that in control tissues binding by soybean agglutinin (sba), dolichos biflorus agglutinin (dba), wheatgerm agglut ...	1989	2769448
biosynthesis and secretion of enamel proteins in the rat incisor.	the synthesis and secretion of enamel proteins (eps) in rat incisors was examined using cytochemical and biochemical methods. radioautography after injection of 3h-methionine showed that ameloblasts in the presecretory, secretory, and maturation stages of amelogenesis actively synthesized and secreted proteins. immunocytochemistry with an antibody to mouse amelogenins revealed the presence of eps in the protein synthetic and secretory organelles of these cells at all three stages. labeling was a ...	1989	2774207
comparison of glycoconjugates at the surface of developing type ii pneumocytes and clara cells.	the apical surface coat of type ii pneumocytes and clara cells in pre- and post-natal rat lung was examined with lectin histochemical methods. lectins from helix pomatia (hpa), peanut (pna) and maclura pomifera (mpa) were conjugated with horseradish peroxidase and used to stain paraffin sections of fixed lung with or without certain pre-treatments. hpa and mpa were observed to react with almost all type ii pneumocytes at postnatal day 1. type ii pneumocytes that stained with a sialidase-pna sequ ...	1989	2777637
detection and isolation of antigen-specific b cells by the fluorescence activated cell sorter (facs).	a method is described for the isolation of antigen-specific b cells from immunized and subsequently boosted mice. antigen-specific b cells were stained by incubation with fluorescein isothiocyanate (fitc)-labelled antigen and then detected and isolated in a fluorescence activated cell sorter (facs). ovalbumin (ova) and helix pomatia haemocyanin (hph) were used as antigens in this procedure, yielding relative amounts of antigen-fitc-binding lymphocytes of 0.9 +/- 0.4% and 3.5 +/- 3.1%. the fitc-p ...	1989	2784157
comparative analysis of agglutinins from hemolymph and albumin gland of helix pomatia.	the hemolymph of helix pomatia contains a weak agglutinating activity. this lectin concentration was calculated to be about 1.8 micrograms.ml-1. among the different red blood cells tested, pronase-treated sheep erythrocytes were found to be the most suitable indicator cells. their agglutination could be inhibited by galnac and glcnac. the serum agglutinin was isolated by affinity chromatography using sephadex g-200 as the matrix. it exhibited a single band in discontinuous page. in the presence ...	1989	2808854
cytochemical and biochemical characterization of glycoproteins in forming and maturing enamel of the rat incisor.	biochemical and histochemical studies have shown the presence of various carbohydrates in enamel. using lectin-gold cytochemistry, we have examined the distribution of glycoconjugates containing n-acetyl-d-galactosamine (galnac) and/or n-acetyl-glucosamine (glcnac)/n-acetyl-neuraminic acid (neunac) residues in rat incisor ameloblasts and in forming and maturing enamel embedded in lowicryl k4m, lr gold, and lr white resins. the enamel proteins that contain these carbohydrate moieties were further ...	1989	2809173
the reaction of nitrogen monoxide with the haemocyanins of the crayfish astacus leptodactylus and the snail helix pomatia.	the rate of the reaction of astacus leptodactylus methaemocyanin with no follows the henderson-hasselbalch equation with a pka of 5.85, suggesting that one imidazole ligand of cu was exchanged for no. the reaction is blocked by f- as a bridging ligand. the same imidazole residue might be responsible for the decomposition of nitrosylhaemocyanin, [cu1no+cuii], with an unlocated binding site for no, into methaemocyanin and no, as the rate increase with ph. no could react preferentially with cua of ...	1989	2818567
the reaction of nitric oxide with copper proteins and the photodissociation of copper-no complexes.	the reactivity with nitric oxide was investigated for a number of type-1, type-2 and type-3 copper proteins azurin from pseudomonas aeruginosa (type-1 copper); bovine superoxide dismutase, diamine oxidase from pig kidney and galactose oxidase from dactylium dendroides (type-2 copper); haemocyanin from helix pomatia (type-3 copper); the blue oxidases ceruloplasmin from pig serum, and ascorbate oxidase from cucurbita pepo medullosa. type-1 copper formed complexes with no in the oxidised state, whi ...	1987	2822126
host cell-induced differences in o-glycosylation of herpes simplex virus gc-1. i. structures of nonsialylated hpa- and pna-binding carbohydrates.	lectins with narrow oligosaccharide specificities were established as probes to study the host cell influence on the biosynthesis of o-linked oligosaccharides of the herpes simplex virus type 1 (hsv-1)-specified glycoprotein c (gc-1). we found that only gc-1 and no other glycoprotein bound to the peanut lectin (pna), with main specificity for gal(beta 1-3)galnac. previously, we have shown that only gc-1 binds to the helix pomatia lectin (hpa), with main specificity for terminal galnac. the o-lin ...	1987	2825412
detection of b and t cells, with lectins or antibodies, in healthy and bovine leukemia virus-infected cattle.	lectins, polyclonal antibodies and monoclonal antibodies (mabs) were evaluated as markers for bovine lymphocytes obtained from healthy animals and from cattle infected with bovine leukemia virus (blv). in the blood from healthy cattle the proportion of cells identified as t lymphocytes with the lectin helix pomatia (hp) (67.8 +/- 6.2%) using the indirect immunofluorescence technique was similar to the proportion of cells identified by the mabs p5 (66.1 +/- 3.8%) and blt-1 (59.8 +/- 7.1%). the pr ...	1988	2839934
[long-term potentiation in the neurons of the edible snail].	a brief high-frequency stimulation of the anal nerve of the isolated nerve ring of snail helix induced a pronounced increase in the amplitude of epsps, evoked in identified neurons of left parietal and visceral ganglions by low frequency (once in 5 min) stimulation of the same nerve. the amplitude of epsp returned to the control level 30-120 min after tetanization. we called this effect long-term potentiation. a brief application of serotonin (10 microm) in the majority of neurons also induced l ...	2010	2850036
membrane currents induced by pentylenetetrazol in identified neurons of helix pomatia.	after systemic application of pentylenetetrazol (ptz), mammalian as well as molluscan neurons generate epileptic paroxysmal depolarization shifts. for a further analysis of these potential oscillations the membrane currents induced by local application of ptz onto identified neurons of helix pomatia were investigated. different types of responses were obtained at membrane potentials negative and positive to ca. -30 mv. at holding potentials more negative than -30 mv, ptz as a rule evoked an inwa ...	2010	2852986
heterogeneity of apical glycoconjugates in kidney collecting ducts: further studies using simultaneous detection of lectin binding sites and immunocytochemical detection of key transport enzymes.	in the search for a functional role for the polarized glycoconjugates of rat collecting duct epithelial cells, the relation between binding of various lectins and expression of cellular transport enzyme profile of the cells was studied. for this purpose, principal and intercalated cells of rat kidney collecting duct were identified by morphological criteria and by their immunocytochemically determined content of (na+ + k+)-atpase and carbonic anhydrase (ca ii), respectively. various n-acetylgala ...	1988	2853698
isolation and identification of a new major metabolite of diflunisal in man. the sulfate conjugate.	a new metabolite of diflunisal has been identified in volunteers and patients after multiple dose administration. the metabolite was isolated from human urine by silica gel chromatography and was further purified by reversed phase hplc. arylsulfatase from helix pomatia and from aerobacter aerogenes completely hydrolyzed the isolated metabolite to diflunisal, although hydrolysis by bacterial arylsulfatase was extremely slow. electron impact mass spectra for diflunisal and its sulfate conjugate we ...	1986	2868855