Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| echinococcus granulosus: identification of subunits of the major antigens. | this paper describes a procedure for obtaining the two "major" echinococcus granulosa antigens (antigens 4 and 5) from sheep hydatid fluid. purified antigen 4 (m.w. 400,000 and over) and antigens 5 (m.w. 150,000) were separated in order to identify molecular subunits of each. sds 12.5% polyacrylamide gel electrophoresis of antigen 4 gave a single band for which a m.w. of 67,000 was calculated. when 5% 2-mercaptoethanol was added to the antigen 4 preparation, sds 12.5% polyacrylamide gel electrop ... | 1977 | 894044 |
| the effect of praziquantel on echinococcus granulosus, taenia hydatigena and taenia ovis infections in dogs. | the effect of praziquantel on echinococcus granulosus, taenia hydatigena and t ovis was investigated in a trial involving 360 dogs. the drug was found to be highly toxic to tapeworms. few dogs remained infected after a single treatment within the range of dose rates tested (0-31 mg/kg to 10-0 mg/kg body weight). no toxicity to dogs was observed. | 1977 | 905644 |
| treatment of hepatic hydatid disease with mebedazole: preliminary results in four cases. | mebendazole was given to four patients with hepatic hydatid disease. in three patients hydatidosis had remained after surgery, and in the fourth it could not be treated surgically. mebendazole was given orally in maximum doses of 400-600 mg three times a day during courses lasting 21 to 30 days. ultrasonic echotomography showed a complete regression of the intrahepatic cysts after four to 13 months in all four cases. in three patients the course of treatment had to be repeated. mebendazole also ... | 1977 | 922416 |
| growth, segmentation and maturation of the british horse and sheep strains of echinococcus granulosus in dogs. | 1977 | 924713 | |
| a re-evaluation of the slide flocculation test for the diagnosis of schistosomiasis with adult worms as antigen. | the present study was conducted to determine whether extracts from adult schistosoma mansoni could be coated onto cholesterol-lecithin crystals. of particular interest was the use of stunted schistosomes (ss) recovered from rabbits. the reactions obtained with this antigen in this slide flocculation (sf) test were compared with those employing antigens from cercariae and mature adult worms. the stunted schistosome sf test (sfss) showed a high sensitivity and specificity while cercarial antigen s ... | 1976 | 937633 |
| immunological response to experimental echnococcosis in meriones unguiculatus. | varying doses of protoscolices of echinococcus granulosus were injected intraperitoneally into mongolian jirds, swiss mice, and golden hamsters. after 4 months the animals were killed and the numbers of developing cysts counted. jirds were by far the most susceptible hosts. doses of 500 protoscolices produced 100% infection rates in both weanlings and adults. comparable infection rates in weanling mice required inocula of 5,000 protoscolices, but even at this dose hamsters were refractory to inf ... | 1976 | 957034 |
| chemotherapy of hydatid disease (echinococcus granulosus) in mice with mebendazole and bithionol. | we treated female swiss webster mice with heavy, long standing infections of echinococcus granulosus with mebendazole, 50 mg/kg body weight daily for 10 days and bithionol, 70 mg/kg every other day for four doses. necropsy performed 6 weeks after completion of therapy showed no gross or histologic differences between untreated controls and bithionol-treated mice or their cysts. mebendazole-treated mice had a significant decrease in the total number of cysts (15 vs. 100), with many of those prese ... | 1976 | 961994 |
| developing protoscoleces of echinococcus granulosus on the outer surface of the brood capsule, detected by scanning electron microscopy. | by means of scanning electron microscopy, stalked protrusions were observed arising from the outer surface of intact brood capsules of echinococcus granulosus. histological studies showed these protrusions to be developing protoscoleces. however, complete development is not attained and the protoscoleces eventually die. it is suggested that external development is a result of overcrowding within the brood capsule. | 1976 | 965709 |
| inhibitory effect of bcg on development of secondary hydatid cysts of echinococcus granulosus. | 1976 | 982765 | |
| ovine cysticercosis in the albany region of western australia. 1. survey of tapeworms of rural dogs. | a survey of the cestode infections of 304 dogs from 134 properties in the albany area of western australia was performed. purgation was induced by oral administration of arecoline and the purge examined for cestodes. the cestodes found and their infection rates were: dipylidium caninum 16.4%; taenia spp 36.5%; t. ovis 8.9%; t. hydatigena 15.1%; t. pisiformis 15.1%; t. serialis 2.3%; echinococcus granulosus 0.7%; questionnaires completed on 133 of the 134 properties at the time the dogs were purg ... | 1976 | 985232 |
| helminth parasites of the fox (vulpes vulpes) in new south wales. | a total of 930 foxes (vulpes vulpes) from new south wales were examined for the presence of gastro-intestinal helminth parasites. the cestodes were represented by taenia pisiformis with a prevalence of 15.7%, t. serialis (22.6%), t. taeniaeformis (0.1%), spirometra erinacei (28.7%) and dipylidium caninum (8.3%). no specimens of t. ovis, t. hydatigena or echinococcus granulosus were encountered. toxocara canis was the most prevalent nematode and occurred in 35.2% of foxes. other nematodes encount ... | 1976 | 985241 |
| an epidemiological approach to the study of echinococcosis in north-east region of iran (khorassan). | we have collected all the reported cases of echinococcus cysts recovered in every surgical ward in mashhad since their establishment. at the same time we searched corpses of livestock which had been slaughtered in khorassan's slaughter houses in different days since 1969 for evidence of cysts, and also dogs, 2 wolves, 4 jackals and 11 foxes which had been killed with strychnine, for adult worms. occurrence of hydatid cyst infestation was found in 478 reports of surgical operations amongst 43,196 ... | 1976 | 1004935 |
| formation of echinococcus granulosus laminated membrane in a defined medium. | 1976 | 1010671 | |
| the role of native domestic animals in the dissemination of echinococcus infection among dogs in the state of kuwait. | infection with hydatid cysts of domestic animals slaughtered by private individuals and at the government central slaughterhouse is recorded. both fertile and non-fertile cysts were seen in the infected animals. the home slaughtered sheep seems to play an important role in the dissemination of the parasite and the life cycle of echinococcus granulosus in the state of kuwait is assumed to be sheep--dog--sheep. | 1976 | 1010925 |
| echinococcus granulosus: development in vitro from oncosphere to immature hydatid cyst. | echinococcus granulosus cysts were cultured in vitro from the oncosphere, and cyst morphogenesis is described. cysts grew to a mean diameter of 16 mm in 120 days, with a maximum of 20 mm. an amorphous layer was apparent around the larvae during early post-oncospheral re-organization. organisms became vesicular at 6 days and laminations appeared in the amorphous layer at 10 days. confluence of proliferating cells to form the syncytium of the germinal membrane was observed. | 1976 | 1012755 |
| the question of diseases caused by echinococcus granulosus and alveococcus multilocularis on the taimyr peninsula. | 1976 | 1014668 | |
| the development of brood capsules and protoscolices in secondary hydatid cysts of echinococcus granulosus. a histological study. | using the mongolian jird (meriones unguiculatus) as a laboratory host for the cystic stage of the british horse strain of echinococcus granulosus, the mode of development of brood capsules and protoscolices was studied histologically. the successive stages in development in secondary hydatid cysts are described and shown to correspond closely with previous observations on primary hydatid cysts. the usefulness of this experimental model for future ultrastructural studies is emphasized. | 1976 | 1016477 |
| [experimental therapy of echinococcosis. 1. laboratory model of echinococcosis and the effect of sarcolysinacridine, levamisole and mebendazole on the development of larval cysts of echinococcus granulosus]. | 1976 | 1025460 | |
| droncit--a milestone in the control of echinococcus granulosus in dogs. | 1976 | 1032462 | |
| the role of the tasmanian devil in the epizootiology of ovine cysticercosis and echinococcosis. | tasmanian devils were fed infective cystic material of taenia ovis, t. hydatigena and echinococcus granulosus and later killed and examined for adult tapeworms. no infections with t. ovis were found after feeding 23 cysts to 8 devils. t. hydatigena were recovered from 5 to 41 devils and 10 worms established after feeding 417 cysticerci. no e. granulosus were recovered from any of 3 devils. specimens of t. hydatigena appeared to develop as well in devils as in dogs. from these and previous result ... | 1976 | 1035096 |
| the effect of bithionol sulphoxide on echinococcus granulosus and taenia hydatigena infections in dogs. | double thiry-vella loops in calves were used to show that when heat stable e coli enterotoxin was in contact with only one of the loops, a net increase in water secretion occured in both. it was concluded that either enterotoxin itself or a mediator substance was absorbed and acted via the systemic circulation. | 1975 | 1090984 |
| effect of ovine hydatid cyst fluid on the cardiovascular and respiratory systems in sheep. | rupture of the hydatid cyst in man brings about mild to severe toxic reactions including death. the present study was undertaken to investigate some of the responses resulting from administration of the ovine hydatid fluid to the sheep, which, like man, is an intermediate host of the echinococcus granulosus. in 50 sodium pentobarbital-anesthetized sheep, the arterial blood pressure (a.b.p.), central venous pressure (c.v.p.), respiration and electrocardiogram were recorded. intraveonus administra ... | 1975 | 1099969 |
| [presence and longevity of hydatid cysts of echinococcus granulosus in imported horses in sweden (author's transl)]. | 1975 | 1114046 | |
| the effect of bithionol sulphoxide on echinococcus granulosus and taenia hydatigena infections in dogs. | trials with bithionol sulphoxide involving 130 dogs, infected with either echinococcus granulosus or taenia hydatigena, indicated that a single treatment at a dose rate of 400 mg/kg or two treatments at 50 mg/kg eliminated the former, but a single treatment at 50 mg/kg was sufficient to eliminate the latter. dose rates 200 mg/kg or more were lethal to some dogs. with two doses of 50 mg/kg, there was only minor discomfort from vomiting or diarrhoea in some dogs. | 1975 | 1118658 |
| the effect of development of taenia hydatigena larvae in the peritoneal cavity of dogs on resistance to a challenge infection with echinococcus granulosus. | 1975 | 1126782 | |
| the lethal effect of mebendazole on secondary echinococcus granulosus, cysticerci of taenia pisiformis and tetrathyridia of mesocestoides corti. | oral administration of mebendazole at a rate of 1 g/kg feed (approximately 50 mg/kg body weight/day) for 14 days killed mature and immature cysticerci of taenia pisiformis in rabbits, and multiplying tetrathyridia of mesocestoides corti in mice. progressive degrees of parasite damage caused by mebendazole treatment could be assessed by histological examination of calcereous corpuscles. a single subcutaneous injection of 10 percent mebendazole in carrier, at a rate of 100 mg/kg body weight, res ... | 1975 | 1128928 |
| immunization of dogs against echinococcus granulosus using worm secretory antigens. | 1975 | 1140879 | |
| echinococcus granulosus: host lymphocyte transformation by parasite antigens. | 1975 | 1149867 | |
| the effect of egg dose, and host age and sex on susceptibility of cf1 albinomice and jirds (meriones unguiculatus) to primary infection by echinococcus granulosus(batsh, 1786). | 1975 | 1153912 | |
| the effect of fospirate on echinococcus granulosus and taenia hydatigena infections in dogs. | trials with fospirate carried out with 170 dogs infected with either echinococcus granulosus or taenia hydatigena indicated that a dose rate of 40 mg/kg given on three occasions and 10 mg/kg given on one occasion eliminated each species, respectively. increasing the dose rat substantially above 40 mg/kg did not reduce the number of treatments required to eliminate all hydatid organisms. vomiting was observed in some dogs. | 1975 | 1166129 |
| the effect of mebendazole on echinococcus granulosus and taenia hydatigena infections in dogs. | the effect of micronised mebendazole on echinococcus granulosus and taenia hydratigena has been investigated in a trial involving 175 dogs. the hydatid organism was eliminated from the dogs at dose rates of either 160 mg/kg given on one occasion or 20 mg/kg given on two occasions. a single dose of 20 mg/kg eliminated t hydratigena. the drug was readily accepted when given in food and there were no untoward sequelae. | 1975 | 1166134 |
| efficacy of bunamidine hydrochloride against immature and mature stages of echinococcus granulosus. | the efficacy of bunamidine hydrochloride (given at dose levels of 25 and 50 mg/kg of body wt) against immature and mature echinococcus granulosus tapeworms was studied in experimentally infected dogs. the compound had average efficacies of 85.9 to 98.8 percent against the immature stages and was completely efficacious (100 percent clearance) against the mature worms. these data indicate that bunamidine hcl at the given doses can be used as a control measure against hydatid disease in the united ... | 1975 | 1169897 |
| comparative value of arecoline hydrobromide and bunamidine hydrochloride for the treatment of echinococcus granulosus in dogs. | a controlled trial involving 122 dogs experimentally infected with echinococcus granulosus was carried out to compare the efficiency of arecoline hydrobromide and bunamidine hydrochloride. a single treatment with arecoline hydrobromide at a dose rate of 4 mg/kg or with bunamidine hydrochloride at 50 mg/kg gave a similar degree of efficiency against young worms. at these dose rates, worms were still present in almost half the dogs at autopsy. | 1975 | 1172623 |
| the effect of nitroscanate on echinococcus granulosus and taenia hydatigena infections in dogs. | trials with nitroscanate carried out on 186 dogs infected with either echinococcus granulosus or taenia hydatigena indicated that a single treatment at a dose rate of 1000 mg/kg or two treatments at 250 mg/kg eliminated the former, but a single treatment at 64 mg/kg was sufficient to eliminate the latter. at this dosing schedule, vomiting and diarrhoea occurred, as well as a transient 'tranquillising' effect on some dogs. | 1975 | 1172624 |
| isolation of the most immunoreactive antigenes of echinococcus granulosus from sheep hydatid fluid. | this paper describes a simplified procedure for obtaining purified echinococcus granulosus antigens from sheep hydatid fluid by using affinity chromatography on concanavalin a-sepharose. the presence of two "major" antigens (4 and 5) was confirmed. antigen 5 was isolated by preparative polyacrylamide gel electrophoresis. antigen 4, eluted by diffusion from the gel, was seen to be "contaminated" by antigen 5 and was isolated by using anti-5 sepharose-linked serum. these two major antigens were th ... | 1975 | 1176778 |
| fine structure of the germinal membrane of echinococcus granulosus cysts. | the ultrastructure of the hydatid cyst germinal membrane was studied. it was divided into 3 regions, the tegument, the tegumental cell region, and the innermost area bordering the cyst cavity. the morphology of tegumental, muscular, flame, duct, and glycogen-containing cells and cells containing lysosomal-like bodies is described. the significance of these findings is discussed in terms of the possible function of these structures and present knowledge on the penetration of macromolecules into h ... | 1975 | 1185424 |
| sylvatic echinococcosis in argentina. i. on the morphology and biology of strobilar echinococcus granulosus (batsch, 1786) from domestic and sylvatic animal hosts. | dogs were fed larvae of echinococcus granulosus (batsch, 1786) recovered from sheep in argentina. the morphological characteristics of strobilae recovered from dogs at 28, 60 and 76 days postexposure were compared with those of echinococcus spp. recovered from naturally infected foxes, dusicyon clupaeus (molina) and d. griseus (gray), and those of worms reared in dogs from larvae in european hares, lepus europaeus (l.). only slight differences were observed in characters of taxonomic importance ... | 1975 | 1189028 |
| studies on effects of drugs upon protoscoleces of echinococcus granulosus in vitro. i. scolicidal effect of salicylanilide and bisphenol derivatives against echinococcus granulosus in vitro. | 1975 | 1195527 | |
| quantitative determination of specific ige antibodies to echinococcus granulosus and ige levels in sera from patients with hydatid disease. | high serum ige levels were found in eighty-nine patients with hydatid disease. specific ige antibodies to e. granulosus hydatid fluid or to a pure e. granulosus antigen were isolated using immunoabsorbents and quantified by a radioimmunoassay. the procedure made it possible to quantify, for the first time, the concentration of ige antibodies in units per millilitre. significant levels of ige antibodies were demonstrated in twenty-three out of thirty-nine patients, by the use of standardized hyda ... | 1975 | 1201857 |
| attempted infection of sheep with echinococcus granulosus equinus. | no evidence of hydatid infection was detected at post mortem examination of sheep which had been inoculated intraperitoneally, four to 11 months previously with protoscoleces of echinococcus granulosus equinus. | 1975 | 1215689 |
| [duration of the life of echinococcus granulosus in the organism of dogs]. | 1975 | 1216506 | |
| letter: transmission of echinococcus granulosus granulosus from kangaroos to domestic dogs. | 1975 | 1222012 | |
| sylvatic echinococcosis in argentina. ii. susceptibility of wild carnivores to echinococcus granulosus (batsch, 1786) and host-induced morphological variation. | domestic dogs and cats, 3 fox species, dusicyon culpaeus (molina,1782), d, griseus (gray, 1837) and d. gymnocercus (thomas, 1914), geoffroy's cats, felis geoffroyi (d'orbigny and gervais, 1843), and grisons, galictus cuja (molina, 1782) were fed larvae of echinococcus granulosus (batsch, 1786) from domestic sheep in argentina. dogs and the 3 species of foxes became infected. the susceptibility of d. culpaeus compared favorably to that of dogs. most d. griseus and d. gymnocercus were less suscept ... | 1976 | 1258144 |
| echinococcosis in arizona and new mexico. survey of hospital records, 1969-1974. | in 1974, seven cases of human echinococcosis were diagnosed in arizona and new mexico. a retrospective survey of arizona and new mexico hospitals obtained data on ten additional cases reported for the 5-year period 1969 through 1973. sixteen cases were diagnosed as echinococcus granulosus infections and one as e. multilocularis infection. the latter infection was in an eskimo from alaska, where e. multilocularis is endemic. all of the 16 e. granulosus cases were probably acquired autochthonously ... | 1976 | 1259090 |
| efficacy of divided doses of fospirate against immature echinococcus granulosus infections in dogs. | seventy dogs were used to evaluate efficacy of divided doses of fospirate against immature echinococcus granulosus infections in dogs. dose rates of 10 to 80 mg/kg given on 1 or 2 occasions resulted in the clearance of 70.6 to 94.5% of expected worm numbers. at least 3 treatments may be required before dogs can be free from e granulosus. vomiting, which occurred in dogs given doses of 40 mg or more/kg, seemed to interfere with anthelmintic efficacy in some dogs. | 1976 | 1275350 |
| efficacy of divided doses of gs-23654 against immature echinococcus granulosus infections in dogs. | one hundred dogs were used to evaluate the efficacy of gs-23654 (4-nitro-4'-isothiocyano-diphenyl-ether) against immature echinococcus granulosus. a 25% suspension of active ingredient was administered at dose rates of 100, 200, or 400 mg/kg of body weight on 1, 2, or 3 occasions. anthelmintic efficacy was dosage dependent and increased with the number of times the dosage was repeated. at none of the treatment schedules tested were all worms eliminated from all dogs, although 92.6% of the expect ... | 1976 | 1275351 |
| analysis of host components in hydatid cyst fluid and immunoblot diagnosis of human echinococcus granulosus infection. | to improve serodiagnosis of cystic hydatidosis, immunoblotting studies were performed to look for a highly specific parasite antigen(s). first, commercially available hydatid cyst fluid antigen preparations were characterized by sds-page and by immunoblotting with sera specific for parasite and host animal proteins. one preparation, designed for use in complement fixation tests, did not appear to be suitable for immunoblotting because of the low concentrations of parasite antigens. several host ... | 1992 | 1288431 |
| [effect of mebendazole on glucose uptake of echinococcus granulosus cysts]. | mice infected with secondary cysts of echinococcus granulosus were treated ig with mebendazole (meb) 25 mg.kg-1.d-1 for 7-14 d. at 24 h after the last dose the endocysts in the treated mice were removed out for in vitro cultivation and exposed to [u-14c]glucose 11.1 kbq.ml-1 for 2 min, no apparent difference in radioactivity content in the cyst walls between the treated and control groups was observed. when [u-14c]glucose was given iv to the infected mice 24 h after they had been treated ig with ... | 1992 | 1300057 |
| effects of mebendazole, albendazole, and praziquantel on alkaline phosphatase, acid phosphatase, and adenosine triphosphatase of echinococcus granulosus cysts harbored in mice. | mice infected with protoscoleces of echinococcus granulosus for 12-14 months were treated ig with mebendazole (meb) 25-50 mg.kg-1 x d-1 for 7-14 d, albendazole (alb) 200 mg.kg-1 x d-1, cr praziquantel (pra) 500 mg.kg-1 x d-1 for 14 d. the mice were killed 24 h after the last medication, and acid phosphatase (acp), alkaline phosphatase (akp), and adenosine triphosphatase (atpase) including (na, k, mg)-atpase, (na, k)-atpase, and (mg)-atpase were determined and compared with those of untreated con ... | 1992 | 1302436 |
| [observation on ultrastructure of sterile cysts of echinococcus granulosus in mice]. | the fine structure of sterile cysts of echinococcus granulosus in mice was studied by tem. the cyst wall consists of laminated layer and germinal membrane. at ultrastructural level, the laminated layer contains microfibrillate matrix and irregularly shaped electron-dense granules. the germinal membrane comprise of tegument and cell region. plasmalemma invagination resembling the "pinocytotic vesicle" are seen between the microtriches of the tegument, and mitochondria are seen in the basal portio ... | 1992 | 1303342 |
| hydatid cyst of the tibia. | a case of hydatid cyst of the tibia, which manifested as a pathologic fracture is being reported. pain and swelling of left lower limb with inability to bear the weight were the main features. tender swelling was also noted at the upper and middle third of tibia. open biopsy revealed the hydatid cyst wall and scolices of echinococcus granulosus. albendazole treatment was followed by curettage and bone grafting. | 1992 | 1307592 |
| [human hydatidosis in rio grande do sul (brazil): an estimate of its significance for the public health of the country]. | by means of data collected through personal visits to hospitals and clinics in the 24 counties of rio grande do sul held to be endemic for echinococcus granulosus an assessment of the importance of this disease is attempted. (barring new developments, these data reflect the situation in the country as a whole). the surgical incidence for 1990 was found to be 5.5 cases per 100 thousand inhabitants, far less than the rates for the neighboring countries. as concerns prevalence, in the absence of ad ... | 1992 | 1342124 |
| [hydatidosis in the ixth region of chile. a regional problem and challenge]. | infection by echinococcus granulosus is the main zoonosis affecting the human population of the ixth region in southern chile. prevalence rates vary from 18.2 to 48 per 100,000. animals are affected with prevalence rates of 40% for bovines, 39.5% for sheep and 14.8% for pork as estimated at the central meat processing plant in the city of temuco. a cost of approximately $300,000 is estimated to treat affected individuals. much greater losses may be estimated from unnotified meat processing in ru ... | 1992 | 1342486 |
| a genetic comparison of human and wildlife isolates of echinococcus granulosus in queensland: public health implications. | to test the hypothesis that the hydatid parasite infecting macropods and dingoes in queensland is a sylvatic strain of echinococcus granulosus, distinct from the domestic strain which produces cysts in sheep and humans. | 1992 | 1346467 |
| cattle strain of echinococcus granulosus and human infection. | 1992 | 1350020 | |
| homeoboxes in flatworms. | a search for homeobox-containing genes was done in the genome of a primitive metazoan, the parasitic tapeworm echinococcus granulosus. five different homeoboxes were isolated, none of them belonging to the classical antennapedia-type. three of the homeodomains are similar to those from the drosophila melanogaster nk-type genes. the fourth homeodomain shares extensive identity with that of the recently reported homeobox-containing gene goosecoid from xenopus laevis. the third helix (the recogniti ... | 1992 | 1359988 |
| further characterization of monoclonal antibodies to echinococcus granulosus antigen 5 and antigen b. | two monoclonal antibodies (24.14, 61a12) to echinococcus granulosus antigen 5 and two (31.15 and 39b3) to antigen b were further characterized using modified sheep hydatid cyst fluid antigens (shcf) in elisa. none of these four monoclonals were directed against carbohydrate or lipid epitopes of shcf antigens since they all reacted strongly with periodate or lipase-treated shcf. on the other hand, they appeared to recognize shcf determinants of protein nature as protease treatment of shcf destroy ... | 1992 | 1379216 |
| [interferon status and the hla antigen system. 1. unilocular echinococcosis]. | in 26 patients with hydatid disease interferons (ifn) blood levels by titration method and hla-antigens by lymphocyte cytotoxicity microtest were estimated. in total group b5 antigen frequency was significantly (p = 0.007 after fischer) higher than in the control group of 155 practically healthy persons: 38.5% and 14.7% respectively. the determination of possible correlation between ifn-alpha and ifn-gamma production, hla-antigens specificity and the localisation of echinococcus granulosus cysts ... | 1992 | 1380632 |
| spontaneous in vitro generation of histamine releasing factor from mononuclear cells of patients with hydatidosis. | we have shown that echinococcus granulosus infection can induce an enhanced sensitivity of basophils to ige-dependent stimuli. to determine whether a cytokine, histamine releasing factor, could account for it, we evaluated 35 patients with active hydatidosis, 5 patients with past e. granulosus infection, and 20 normal volunteers. the spontaneous production of a factor in vitro that provoked the release of histamine from basophils was observed by mononuclear cells from patients with active diseas ... | 1992 | 1382747 |
| [isolation of germinal cells from the secondary cysts of echinococcus granulosus harbored in mice]. | the secondary cyst tissues derived from mice infected with protoscoleces of echinococcus granulosus for 8-10 months were digested with 0.25% trypsin at 37 degrees c for 30 min. the separation of different cells in the remaining suspension was achieved by discontinuous gradient centrifugation. the germinal cells were washed 3 times with ice-cold hbss, and then cultivated in the medium of rpmi 1640 supplemented with 20% of calf serum. the cells were kept in an incubator at 37 degrees c in an atmos ... | 1992 | 1394914 |
| comparative immunoelectrophoretic analysis of echinococcus granulosus, taenia hydatigena and taenia pisiformis cyst fluid antigens by hyperimmune rabbit sera. | cyst fluid antigens of echinococcus granulosus, taenia hydatigena and t pisiformis were examined by electrophoresis using homologous and heterologous hyperimmune rabbit sera to these antigens. while arc 5 forming antibodies were identified in sera from rabbits immunised with e granulosus and t hydatigena cyst fluids, antibodies responsible for forming precipitating antigen b band were detected in rabbit antisera to e granulosus, t hydatigena and t pisiformis antigens. t hydatigena cyst fluid app ... | 1992 | 1410812 |
| frequency distribution of echinococcus granulosus in dog populations in the xinjiang uygur autonomous region, china. | age-prevalence and age-intensity data of echinococcus granulosus in dog populations were collected in four counties in the xinjiang uygur autonomous region, people's republic of china. the frequency distribution of the parasite per dog was adequately described by the negative binomial model, with parameter k being 0.0571. the mathematical model of the life cycle of e. granulosus constructed by roberts et al. (1986--parasitology, vol. 92, pages 621-641) was used to estimate the epidemiologic para ... | 1992 | 1413455 |
| a survey of taeniid cestodes in farm dogs in mid-wales. | in 1983-1988, dogs on 315 farms in powys, mid-wales, were purged once with arecoline acetarsol and the faecal purges were examined for cestodes. a few dogs were examined by autopsy. ninety-seven farms had at least one infected dog, and cestodes were found in 161 of the 882 dogs examined. the ovine strain of echinococcus granulosus was found in 41 dogs on 25 farms. taenia hydatigena, t. pisiformis, t. multiceps, t. serialis, t. ovis and dipylidium caninum were recovered from 85, 35, 15, 3, 19 and ... | 1992 | 1417204 |
| the cestodes of foxhounds and foxes in powys, mid-wales. | cestodes were found in 129 of 875 foxhounds from 20 packs in powys, mid-wales, examined by purging in 1983-1988, in the following order of prevalence: taenia hydatigena in 57 hounds (6.51%), echinococcus granulosus in 27 (3.09%), dipylidium caninum in 25 (2.86%), t. ovis in 14 (1.60%), t. pisiformis in 10 (1.14%), t. serialis in five (0.57%) and t. multiceps in four (0.46%). of 197 foxes examined by autopsy, 22 harboured cestodes: two (1.02%) had e. granulosus, nine (4.57%) t. polyacantha, eight ... | 1992 | 1417205 |
| echinococcus granulosus: study of the in vitro complement activation by protoscoleces by measuring the electric potential difference across the tegumental membrane. | complement activation by protoscoleces of echinococcus granulosus was studied by analyzing the damage to their tegumental membrane produced by incubation in both normal and hydatid human sera. the state of the apical tegumental membrane was evaluated by measuring the electric potential difference with microelectrodes. protoscoleces incubated in ringer-hepes or in heat-decomplemented normal human serum in the presence or absence of specific antibodies did not show significant variations in the el ... | 1992 | 1426130 |
| evaluation of different immunodiagnostic techniques for diagnosis of hydatidosis in egypt. | in this study detection of the circulating hydatid antibodies was performed by three serological tests: immunoelectrophoresis (iep), enzyme linked immunosorbent assay (elisa) and enzyme linked immunoelectrotransfer blot (eitb) technique. the antigen which was used in these tests was echinococcus granulosus antigen free of host (camel) antigen by immunoaffinity chromatography. most serum samples were pre-selected from the patients on the basis of clinical syndrome or past history of possible diag ... | 1992 | 1431284 |
| genetic variants within the genus echinococcus identified by mitochondrial dna sequencing. | the pattern of species and strain variation within the genus echinococcus is complex and controversial. in an attempt to characterise objectively the various species and strains, the sequence of a region of the mitochondrial cytochrome c oxidase subunit i (co1) gene was determined for 56 echinococcus isolates. eleven different genotypes were detected, including 7 within echinococcus granulosus, and these were used to categorise the isolates. the 4 generally accepted echinococcus species were cle ... | 1992 | 1435857 |
| in vitro culture of the strobilar stage of echinococcus granulosus of sheep and donkey origin from jordan. | protoscolices of echinococcus granulosus isolated from hydatid cysts of sheep and donkeys in jordan were cultured in vitro using a modified diphasic culture system. protoscolices from these two sources manifested differences in the mode of development, evagination and growth rates. protoscolices isolated from sheep cysts grew in vitro in the polyzoic direction up to the three- to four-segmented mature worms, reaching a length of 2.9 mm. in contrast, donkey protoscolices failed to develop beyond ... | 1992 | 1438153 |
| serological evaluation of the 12 kda subunit of antigen b in echinococcus granulosus cyst fluid by immunoblot analysis. | this study evaluated the 12 kda (smallest) subunit of echinococcus granulosus antigen b as a diagnostic molecule. using immunoblotting, 90.9% of cystic hydatid patients, 40% of alveolar hydatid patients and 5.5% of cysticercosis patients showed sero-reactivity to this subunit. human antibody response to the 12 kda molecule appeared independent of factors such as parasite strain or host population responsiveness. the majority of infection sera, and some normal human controls, also recognized the ... | 1992 | 1440787 |
| frequency distribution of echinococcus granulosus hydatid cysts in sheep populations in the xinjiang uygur autonomous region, china. | age-prevalence and age-intensity data of echinococcus granulosus hydatid cysts in sheep populations were collected in an abattoir in the xinjiang uygur autonomous region, people's republic of china. the frequency distribution of the larval cysts per sheep was empirically described by the negative binomial model, with parameter k being 0.5273. a mathematical model for the life cycle of e. granulosus was applied to the collected data and the results show that the infection pressure on sheep was 0. ... | 1992 | 1441193 |
| the efficacy of chemotherapy with mebendazole in human cystic echinococcosis: long-term follow-up of 52 patients. | sixty patients with echinococcus granulosus infection of the liver, lungs, bone and/or soft tissues were treated for several months with oral mebendazole (50-60 mg kg-1 day-1), in divided doses after fat-rich meals, either without surgery (ws), post-surgery (ps) or pre- and post-surgery (pps). long-term follow-up, possible for 52 of the patients, showed that ws, ps and pps patients have so far remained disease-free following treatment for (means +/- standard deviations) 65.5 +/- 37.7, 82.5 +/- 3 ... | 1992 | 1449273 |
| the role of foxes vulpes vulpes in the epidemiology of echinococcus granulosus in urban environments. | to survey the prevalence of intestinal worms, particularly echinococcus granulosus, in foxes in canberra. | 1992 | 1454000 |
| a strategy for production of monoclonal antibodies to echinococcus granulosus antigen 5 and antigen b. | serum antibody responses to sheep hydatid cyst fluid (shcf) and a purified antigen 5 (ag5) were examined in elisa, immunoelectrophoresis (iep) and immunoprecipitation (ip) to facilitate production of monoclonal antibodies (mab) to e. granulosus ag5 and antigen b (agb). although sera from mice immunized with shcf contained antibodies of various classes, the fusions using these donor mice resulted in mainly anti-agb mab, possibly due to the preferential selection of mab to agb by the shcf-based el ... | 1992 | 1459778 |
| echinococcus granulosus in sheep: transfer from ewe to lamb of 'arc 5' antibodies and oncosphere-killing activity, but not protection. | fourteen ewes were orally dosed with 2000 e. granulosus eggs at 2 weeks of age, were mated at 19 months, and produced lambs when the cysts were 2 years old. one week after parturition, all 14 ewes had 'arc 5' antibodies in their serum, as did 11/14 of their lambs. fourteen uninfected ewes were immunized three times before parturition with e. granulosus eggs injected intramuscularly. cysts grew at the first, or first and second site, but not the third, indicating that the ewes were immune prior t ... | 1992 | 1459779 |
| prevalence of human hydatid disease in northwestern libya: a cross-sectional ultrasound study. | a total of 4103 people were screened in an ultrasound survey of the prevalence of hydatid disease (echinococcus granulosus) in five areas of northwestern libya; hydatid cysts were seen in 57 (1.4%), an overall prevalence of approximately 2.0% when adjusted for the likelihood of the occurrence of cysts in other sites in the body. all ultrasound-positive cases were confirmed by dot-blot elisa. the prevalence of hydatid cysts increased with age, and differed between the sexes except in the five to ... | 1992 | 1463358 |
| helminth parasites of carnivores in northern iran. | a total of 100 jackals, 35 stray dogs, 13 cats, two badgers, one fox and one wild cat were collected from two provinces in northern iran. on post mortem examination 41 species of helminths were recovered, at least 12 of which have been reported infecting humans in various countries. hookworms were the most commonly found parasites, and echinococcus granulosus showed the highest intensity of infection. seven helminth species from jackals, three species from dogs, four species from cats and four s ... | 1992 | 1463360 |
| preliminary observations on helminth parasite populations of the dromedary in northern mali. | an abattoir survey on gastrointestinal parasites of the dromedary (camelus dromedarius) was carried out in march/april 1987 in northern mali. parasitological examination of the digestive tract contents of 11 dromedaries revealed that haemonchus longistipes was the predominant parasite with a prevalence as high as 85.7%. the genera impalaia, trichostrongylus, cooperia, and oesophagostomum were also recovered, but with a lower level of infection and frequency. the larval stage of the canine cestod ... | 1992 | 1466143 |
| use of echinococcus granulosus worm antigens for immunodiagnosis of e. granulosus infection in dogs. | echinococcus granulosus worm excretory/secretory antigens (wes) were used in elisa for diagnosis of e. granulosus infection in dogs and compared with protoscolex somatic antigens (psm). sera from 224 dogs were tested. there was no correlation between elisa absorbance values and e. granulosus worm burdens using either antigen. there was a significant linear relationship between absorbance values of sera tested in the elisa using wes (w-elisa) and the elisa using psm (p-elisa). however, there was ... | 1992 | 1485424 |
| limiting dilution analysis of autoreactive t cells in patients affected by hydatid disease. | we evaluated the peripheral autoreactive response in patients with echinococcus granulosus compared with healthy individuals. a limiting dilution analysis (lda) was performed to estimate frequency of auto-reactive t cells and to detect the complex mechanism that often underlies the autoreactive response. different lda curves were seen in healthy controls and patients. the control group provided a straight line passing through the origin ('single hit' kinetics), indicating that only peripheral au ... | 1992 | 1489486 |
| echinococcus granulosus infection of the liver and lung in an 8 1/2-year-old boy. | we report on an 8 1/2-year-old boy with an echinococcus granulosus infection of the liver and lung, drawing particular attention to the therapy applied. this therapy consists on the one hand of medicinal treatment with mebendazol and on the other hand of two subsequent operations with resection of the cysts. the course of the illness is illustrated by means of the most important laboratory parameters; an indication of the present serological diagnostic is also given. | 1992 | 1498102 |
| molecular and immunological diagnosis of echinococcosis. | echinococcosis is an infectious disease of humans caused by the larval (metacestode) stage of the cestode species echinococcus granulosus (cystic echinococcosis or hydatid disease) or echinococcus multilocularis (alveolar echinococcosis or alveolar hydatid disease). clinical manifestations depend primarily on localization and size of hepatic lesions and may include hepatomegaly, obstructive jaundice, or cholangitis. prognostically, alveolar echinococcosis is considered similar to liver malignanc ... | 1992 | 1498767 |
| identification and characterization of specific hydatid antigen fraction(s). | a specific hydatid antigen was prepared in this study from echinococcus granulosus cyst in livers and lungs of camels. elimination of host "camel" protein from crude hydatid fluid was achieved by two methods: salting out using ammonium sulfate precipitation method and immunoaffinity purification using coupled anticamel antibody to cyanogenbromide activated sepharose 4b gel. testing the prepared hydatid antigen against anticamel serum, using immunodiffusion method, indicated that the affinity pur ... | 1992 | 1500792 |
| [echinococcosis in pregnancy]. | hydatid disease affects sheep and cattle and is caused by the larval form of echinococcus granulosus. affected human beings are only chance intermediate hosts. we present a woman admitted in her 22nd week of pregnancy because of upper abdominal pain and persistent vomiting. the presumptive diagnosis of hydatid cyst of the liver was established by ultrasound, x-ray and ct examinations. at laparotomy a 10 x 7 cm hydatid cyst was removed from the liver. she recovered completely and delivered sponta ... | 1992 | 1505822 |
| diagnosis of echinococcus granulosus infection in dogs. | 1992 | 1509663 | |
| [environmental hygienic aspects of echinococcosis]. | a literature review on the current situation of echinococcosis in central europe is given. the only final host for echinococcus granulosus in this region ist the dog. the infection rate of dogs with e. granulosus in central europe is less than 1%. according to meat inspection statistics in germany less than 0.008% of sheep, pigs and horses carry larval stages of e. granulosus. parasitologically confirmed is the occurrence of a cattle-dog strain, the cysts of which were found in 0.26% of slaughte ... | 1992 | 1516550 |
| evaluation of eight serological tests in the diagnosis of human echinococcosis and follow-up. | the elisa with use of igg, iga, and ige, latex agglutination, indirect hemagglutination (iha), total ige, the radioallergosorbent test (rast), and immunoelectrophoresis (iep) were carried out to determine the preoperative diagnosis of infection due to echinococcus granulosus in 131 patients. eighty-nine patients received follow-up care for 42 +/- 22 months (mean +/- sd); 72 were treated surgically and 17 with mebendazole only. we analyzed 5 +/- 2 serum samples per patient and analyzed each serum ... | 1992 | 1520796 |
| the prevalence of echinococcus granulosus in stray dogs in iraq. | the present paper records the prevalence of echinococcus granulosus in stray dogs of al-tamim (northern iraq), diala (mid iraq) and theqar province (southern iraq), where many people are infected with hydatid disease. of 150 dogs examined in three provinces in iraq, 57 (38%) were infected with e. granulosus. the prevalence of the worm was higher in the dogs of theqar province (56%) than in those of al-tamim (20%) and diala provinces (38%). infections were light (1-200 worms) in eight (14%) of th ... | 1992 | 1560486 |
| echinococcus granulosus: possible formation of a shelled egg in vitro. | what appeared to be the early stages in the formation of a single egg with a striated embryophore was observed in an in vitro culture of echinococcus granulosus protoscoleces isolated from sheep hydatid cysts in north jordan. the 'egg' measured 19 x 19 microns in diameter and was formed in an intermediate vesicular/monozoic form which was never previously reported from a culture. this is the first report of an apparently shelled egg forming in an in vitro culture, but although promising, cannot ... | 1992 | 1563913 |
| sylvatic hydatid disease in children: case reports and review of endemic echinococcus granulosus infection in canada and alaska. | 1992 | 1565558 | |
| hydatid disease in a horse. | during routine necropsy of a 28-year-old horse with intestinal volvulus, numerous hydatid cysts were discovered in the liver. viable protoscolices of echinococcus granulosus were obtained from the cyst. as a 4-year-old, this horse had been imported from an area that is enzootic for equine hydatidosis. | 1992 | 1577650 |
| interventional radiology in the liver and pancreas. | the application of interventional radiographic procedures in the management of patients with liver and pancreatic pathology continues to expand. percutaneous hepatobiliary interventional procedures that have received considerable attention in the past year include technical refinements of the transjugular intrahepatic portosystemic shunt procedure used in patients with portal hypertension and esophageal varices, transshunt embolotherapy of persistent varices in patients with small-caliber mesoca ... | 1992 | 1581136 |
| [metastatic echinococcosis as a rare differential diagnosis of therapy refractory bronchitis]. | in a 68 year old male german patient we found a transdiaphragmatic perforation of a superinfected liver echinococcus granulosus cyst into the bronchial system as cause of a purulent bronchitis resistant to treatment. after operative removal and oversewing of diaphragm bronchitis was cured and the patient could be discharged. | 1992 | 1584736 |
| a clinical-radiological evaluation of benzimidazoles in the management of echinococcus granulosus cysts. | nine patients with complicated hydatid disease managed with surgery and mebendazole/albendazole are presented. five patients received albendazole (1 treatment course) and 5 patients received mebendazole (3 had 2 treatment courses, 1 had a switch-over from mebendazole to albendazole). the mean durations of treatment and follow-up were respectively 7 +/- 2.5 months and 7 +/- 2.5 months (albendazole); 13 +/- 10 months and 29 +/- 31 months (mebendazole). a superior clinical and radiological response ... | 1992 | 1589713 |
| evaluation of a monoclonal antibody-based competition elisa for the diagnosis of human hydatidosis. | an antibody competition enzyme-linked immunosorbent assay (elisa) using 4 different monoclonal antibodies (mab) raised against major antigens (antigen 5 and antigen b) of echinococcus granulosus was evaluated for the diagnosis of human hydatidosis. the competition assay, using anti-ag5 mab 24.14, detected specific antibodies in 70% (131/188) of sera from patients with surgically confirmed e. granulosus infection and 38.5% (10/26) of sera from patients with e. multilocularis infection. none of th ... | 1992 | 1594299 |
| immunodiagnosis of human cysticercosis (taenia solium): a field comparison of an antibody-enzyme-linked immunosorbent assay (elisa), an antigen-elisa, and an enzyme-linked immunoelectrotransfer blot (eitb) assay in peru. the cysticercosis working group in peru (cwg). | we compared results of an enzyme-linked immunosorbent assay (elisa) and an enzyme-linked immunoelectrotransfer blot (eitb) assay for the diagnosis of cysticercosis in sera and cerebrospinal fluid (csf). sera from 34 patients with confirmed cysticercosis were tested by both elisa and eitb assays. cerebrospinal fluid from some of these patients was also tested by elisa for the presence of antibody (ab-elisa) (n = 21) and antigen (ag-elisa) (n = 15). specificity in sera was examined by testing 51 s ... | 1992 | 1599056 |
| [bone hydatidosis. 12 cases]. | the hydatic cyst is a parasitic disease caused by the development in man of the larval form of echinococcus granulosus. all parts of the body may be involved, but the liver and lungs are the main locations of the disease. the bone site is rare, accounting for 0.9 to 2% of all hydatic lesions. between 1982 and 1988, we have compiled 12 cases of hydatic disease of bone. they were located in the skull (4 cases), the iliac bone (4 cases), the omoplate (1 case), the ribs (1 case), the femur (1 case) ... | 1992 | 1599580 |
| immunological localization of blood pl antigen in tissues of echinococcus granulosus. | 1992 | 1616399 | |
| enzyme-linked immunoelectrotransfer blot test for diagnosis of human hydatid disease. | sera from 71 patients with surgically confirmed hydatid disease (which is caused by echinococcus granulosus) were studied by an enzyme-linked immunoelectrotransfer blot (eitb) assay. sera from patients either with other cestode infections or with another illness were used as controls. results of the eitb test for hydatidosis were compared with those of the double-diffusion (dd5) test and an enzyme-linked immunosorbent assay (elisa). in the eitb assay with bovine lyophilized hydatid fluid, three ... | 1992 | 1624574 |
| examination of murine antibody response to secondary hydatidosis using elisa and immunoelectrophoresis. | antibody responses in mice with up to 64 weeks of secondary echinococcus granulosus hydatidosis were examined by elisa using hydatid protoscolex antigen (px), antigen 5 (ag5) and antigen b (agb), and by immunoelectrophoresis (iep) using sheep hydatid cyst fluid (shcf). anti-px igg antibodies, evident from 3-5 days post infection (p.i.), increased steadily until 16 weeks and maintained a high level afterwards. anti-ag5 igg antibodies were negligible up to two weeks, but they showed a small increa ... | 1992 | 1625903 |
| cyclophilin of schistosoma japonicum. | a 623-bp cdna molecule encoding cyclophilin, a specific cyclosporin a-binding protein, has been isolated from schistosoma japonicum using a heterologous cdna probe from echinococcus granulosus. the nucleotide sequence of this molecule has been determined, and the deduced amino acid sequence has revealed extensive homology with homologues of other species. southern blot analysis suggests that s. japonicum cyclophilin is the product of a single-copy gene. the cloning of this cdna will allow an inv ... | 1992 | 1635025 |