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contact sensitivity responses in mice infected with trypanosoma cruzi.mechanisms of depression of contact sensitivity responses in c57bl/10 mice infected with trypanosoma cruzi were studied. cellular involvement during sensitization with oxazolone was investigated in mice acutely infected with t. cruzi. contact sensitivity was not expressed in mice during the latter stages of the acute infection. spleen cells from sensitized, infected mice which were unable to respond to oxazolone could confer contact sensitivity upon normal syngenic mice as effectively as spleen ...1978103835
the course of infection of trypanosoma cruzi in gamma-irradiated rats.the course of infection of both blood and tissues by the sonya strain of trypanosoma cruzi in gamma-irradiated rats has been investigated. the blood infection was dimorphic; slender and broad forms were present throughout, though broad forms predominated at high parasitaemias. both total blood parasitaemia and numbers of slender forms increased progressively in a series of waves with a periodicity of about four days. the rate of increase in the numbers of intracellular parasites corresponded wit ...1978104411
[epidemiology of chagas disease in chile. housing conditions related to the presence of triatoma infestans and the rate of humans and animals infected by trypanosoma cruzi (author's transl)]. 1978104721
hematotropic parasites of translocated raccoons in the southeast.ninety-four raccoons (procyon lotor) from 6 southeastern states were examined for hematotropic parasites, to evaluate the carrier potential of these animals as they relate to translocation and release for hunting purposes. four species of hematotropic parasites were identified, viz, babesia procyonis, haemobartonella procyoni, hepatozoon procyonis, and trypanosoma cruzi. lesions in infected raccoons were associated only with h procyonis. babesia procyonis was enzootic in populations of raccoons ...1978104938
reduction of complement levels in mice infected with trypanosoma cruzi. 1978105116
[experimental infection of the rabbit by trypanosoma cruzi]. 1978105224
active entry of bloodstream forms of trypanosoma cruzi into macrophages.the uptake of bloodstream forms of trypanosoma cruzi, y and cl stocks, by mouse peritoneal macrophages and their intracellular differentiation and multiplication has been compared in vitro. after 48 h the number of macrophages showing intracellular amastigote forms was higher when the y stock was used. the number of parasitized cells increased with the time of contact between parasites and macrophages. prior treatment of the parasites with anti-t. cruzi antibodies and/or complement increased the ...1979105337
epidemiologic studies of chagas' disease in oaxaca, mexico. 1998105774
trypanosoma cruzi: acetylcholine content and cholinergic innervation of the heart in rats. 1979105922
the lysis of trypanosoma cruzi epimastigotes by eosinophils and neutrophils. 1978107139
acquired cell-mediated immunodepression in acute chagas' disease.in this study two groups of patients with acute chagas' disease were identified. group one consisted of five patients with apparent acute chagas' disease. these patients showed symptoms and signals of an acute illness, such as high fever and enlarged spleen. one of these patients developed severe myocarditis and heart failure. group two consisted of seven patients with inapparent acute chagas' disease. this was a nonclinical entity, not perceived by the patient who did not seek medical care. the ...1978107195
chemotherapeutically acitve nitro compounds. 4. 5-nitroimidazoles (part iii).more than 100 1-methyl-5-nitroimidazoles substituted in the 2-position via an oxymethyl group were synthesized and their structure-activity relationship toward various protozoa was investigated. among the derivatives substituted with an aromatic radical there are most of the compounds which are highly effective against trichomonads; 9 preparations are superior to tinidazole and 29 are superior to metronidazole in mice infected with trichomonas fetus, and 9 compounds exhibit a better effect than ...1978107955
[congenital chagas' disease in a non-endemic area]. 1998108501
[arterial pressure in 18-year-old youths in an endemic area of chagas' disease]. 2004108503
immunology of chagas' disease. iii--absorption of human chagasic sera with epimastigotes of different strains of trypanosoma cruzi. 1978108797
antibodies against neurons in chronic chagas' disease.severe qualitative and quantitative alterations of the autonomic system inervating the heart and other organs have been demonstrated in experimental and natural chagas' disease. these facts led us to search for the presence of antibody to neurons in 230 chagasic sera by an indirect immunofluorescence technique. our results indicated that 83% of the chagasic patients presented igg antibodies and 7% igm antibodies to neurons. all control sera were negative. the demonstrated antibody to neurons pre ...1998108824
electromyographical findings in human chronic chagas' disease.an electromyographical investigation of 80 patients with chronic chagas' disease was made. it was found that 79% of the studied patients had emg manifestations of old and chronic denervation of the upper and lower limbs without clinical features of nervous system involvement.1978109065
an electrophysiological investigation of skeletal muscle in human chronic chagas' disease.an electrophysiological study has been made of the thenar, hypothenar, soleus and extensor digitorum brevis muscles and their inervation in 90 patients with chronic chagas' disease. some of them showed a reduced number of functional motor units with increased size of many of the surviving units. no decremental muscle response was found to repetitive nerve stimulation. motor and sensory conduction velocities as well as motor terminal latencies were on the normal range. these findings suggested th ...1978109066
gastrointestinal pathology in south america.non-neoplastic gastrointestinal disease in south america is largely related to environmental conditions. parasitic disorders, including chagas' disease with megacolon, predominate in endemic regions. common enteritides of various etiologie are frequent, whereas appendicits, diverticulosis of the colon, ulcerative colitis and crohn's disease are extremely rare. there was no appendicitis in native indians of paraguay.1998109417
suppressor cells present in the spleens of trypanosoma cruzi-infected mice.infection with trypanosoma cruzi decreases the ability of spleen cells from mice to respond to either t cell, concanavalin a (con a), or b cell, lipopolysaccharide (lps), mitogens. the effect of infection on the mitogenic response depends on the elapsed time between the day of infection and the time of mitogen presentation. responses early in infection are normal, whereas later responses to either mitogen are depressed. spleen cells from late trypanosome-infected mice inhibit the ability of norm ...1979109508
chemotherapy with ethidium bromide-dna complex in established chagas' disease.two different strains of mice (akr and nmri-ivic) were inoculated intraperitoneally with the virulent y strain of trypanosoma cruzi, and then treated with the lysosomotropic ethidium bromide-dna complex, according to several different treatment schedules. when animals were treated 48 hours after intraperitoneal inoculation with three intraperitoneal doses of eb-dna no parasitemia was detected, even after 11 weeks, confirming previous results. however, when infection was allowed to become fully e ...1979110160
prevalence of parasitemia and seroreactivity to trypanosoma cruzi in a rural population of northeast brazil.age-specific prevalence rates of parasitemia and seroreactivity to trypanosoma cruzi were determined in a rural area endemic for chagas' disease in northeast brazil. parasitemia was detected by blood cultures and xenodiagnosis, and serum antibodies to the parasite were measured by the complement fixation (cf) and indirect immunofluorescence (ifa) tests. of the 116 persons examined, 39 (33.7%) had antibodies and 23 (19.8%) had parasitemia. ninety-six percent of parasitemic individuals were seropo ...1979110161
the importance of clinical, psychological, and social effects experienced by patients with american trypanosomiasis (chagas' disease). 1998110383
antibody-dependent cytolysis of trypanosoma cruzi by human polymorphonuclear leukocytes. 1979110466
[esophageal motor changes in chagas' disease in venezuela]. 2006110643
thymus-dependent control of host defense mechanisms against trypanosoma cruzi infection.congenitally athymic homozygous (nu/nu) mice were shown to be significantly more susceptible to trypanosoma cruzi infection than their thymus-bearing heterozygous (nu/+) littermates, as measured by increased parasitemia, mortality rate, and shortened survival time. in addition, transplantation of neonatal thymus into athymic mice reestablished normal levels of resistance to t. cruzi, i.e., comparable to those of normal littermates. these results constitute conclusive evidence that host defense m ...1979110686
american trypanosomiasis: chagas' disease. 1979110719
screening and structure-activity relationships of synthetic juvenile hormone analogues for panstrongylus megistus, a primary vector of chagas' disease in brazil. 1978110859
[wild reservoirs and vectors of trypanosoma cruzi. lxxi: comparative study on the importance of the port of entry of metacyclic trypomastigotes in experimental t. cruzi infection]. 1979111308
[wild reservoirs and vectors of trypanosoma cruzi. lxxiii: resistance against wild strains of t. cruzi in mice inoculated with herpetomas samuelpessoai]. 1979111309
[wild reservoirs and vectors of trypanosoma cruzi. lxxii: behavior of the pf strain of t. cruzi in mice inoculated with flagellates obtained from triatominal infected by artificial xenodiagnosis or by inoculation into the coelomic cavity]. 1979111310
the protective effect of non-immunologic granulomatous inflammation in trypanosoma cruzi infection of mice. 1979111311
[early immunological aspects in rats infected by trypanosoma cruzi. ii. soluble antigen circulation and the modifications of serum complement from animals in sucessive days of the infection (author's transl)].a group of rats was infected by the y strain of t. cruzi and three animals were bled daily from the 4th to the 13th days. the sera obtained each day, after being mixed, were used for dosing serum complement and for acting as antigens in the cft with a chagas' disease serum. the amount of circulating antigen increased gradually, reached a maximum in the 5th and 6th days and then decreased up to the 10th day; thereafter it increased attaining a new maximum in the 11th day and again decreased up to ...1979111312
[survey of chagas disease in different blood banks using the indirect immunofluorescent test (author's transl)]. 2000111327
[chagas disease of the digestive tract (author's transl)]. 2000111328
[prevalence of trypanosoma cruzi infection, in 1975, in 2 blood banks of londrina, paraná, brazil]. 1978111340
[focal point investigation, 1 activity of the campaign of control of transmitters of american trypanosomiasis]. 1978111342
disease and development in the tropics: a review of chagas' disease. 2000111352
in vivo and in vitro interaction of trypanosoma cruzi with mouse macrophages and heart muscle cells--film presentation. 1979111486
improvements in routine xenodiagnosis with first instar dipetalogaster maximus (uhler 1894) (triatominae).first instar dipetalogaster maximus proved as effective as third instar triatoma infestans in detecting circulating trypanosoma cruzi in patients with chronic infections examined in a routine xenodiagnostic laboratory. since rearing costs are diminished, d. maximus has now replaced t. infestans as the xenodiagnostic agent in our laboratory. however, further work is needed to define the optimal conditions necessary when using this species in xenodiagnosis.1979111570
[demonstration of re-entry in the his-purkinje system in chronic chagas' disease. possible mechanisms of tachyarrhythmia]. 1979111650
[thyroid function in chronic chagas' disease. evaluation of trials with in vivo and in vitro radionuclides]. 1979111651
circulating antibodies to peripheral nerve in american trypanosomiasis (chagas' disease).an antibody reacting with schwann sheaths of myelinated somatic and unmyelinated autonomic peripheral nerve was found in sixty-one out of seventy-one chronic, and nine out of ten acute, chagas' disease sera. indirect immunofluorescence (ifl) was carried out on rat, mouse and human somatic nerves and rat sympathetic nerves with initial serum dilutions of 1 : 10, and the staining reached a final titre of 1 : 320 in some cases. the antibodies fixed complement and were absorbed out by lyophilized ep ...1979111884
studies of the domestic ecology of triatoma infestans by means of house demolition. 1979112663
[action of aspergillus sp. on trypanosoma cruzi in vitro]. 1979112665
[transmission potential of chagas' disease by blood transfusion: serological findings among donors of the state of oaxaca]. 2006112696
chagas's disease in the amazon basin: 1. trypanosoma cruzi infections in silvatic mammals, triatomine bugs and man in the state of pará, north brazil.a total of 1,197 wild animals from pará state north brazil, were examined for haematozoa. trypanosoma cruzi-like parasites were found in 13 different species, and were particularly common in a variety of marsupials (didelphidae), porcupines (coendou spp.), armadillos (dasypus novemcinctus) and coatimundis(nasua nasua). three human infections are reported, making a total of seven autochthonous cases of chagas's disease from pará since the first were described in 1969. a serological survey of 5,31 ...1979112730
enhancement of host resistance against trypanosoma cruzi infection by the immunoregulatory agent muramyl dipeptide.n-acetylmuramyl-l-alanyl-d-isoglutamine (muramyl dipeptide [mdp]) enhanced resistance against trypanosoma cruzi infection in mice. this effect was evidenced by significant reductions in both parasitemias and mortality rates and increased survival time in mdp-treated animals compared with untreated infected mice. mdp effectively augmented host resistance when administered in any one of the following ways: (i) continuous subcutaneous release from an osmotic minipump for a 7-day period starting 2 d ...1979113344
antibody-dependent cellular cytotoxicity of trypanosoma cruzi: characterization of the effector cell from normal human blood.the antibody-dependent cellular cytotoxicity activity of normal human blood cells against epimastigotes of trypanosoma cruzi was measured by the release of incorporated [3h]uridine. sera from patients with chronic chagas' disease were used to sensitize the parasites to the lytic activity of the effector cells. different steps of peripheral blood cell purification were employed, and different cell subpopulations were tested as effectors in the system. the main cytotoxic activity was detected in t ...1979113345
[evaluation of the radioimmunoassay applied to the serological study of chagas' disease]. 2002113857
[gastric secretion in patients with chagas' disease]. 1979113858
[circulating immune complexes in experimental chagas' disease. identification of parasitic antigens in the complexes]. 1979113861
celisa (complement-enzyme linked immuno sorbent assay) a new method for the estimation of complement fixing antibodies; its use for chagas' disease.a description is given of a new method for the estimation of complement fixing antibodies. this is a modification of the enzyme linked immuno sorbent assay (elisa) to a complement-enzyme linked immuno sorbent assay (cilisa) using an anti-c3 conjugate. the method is independent of the haemolytic system. to test the sensitivity of celisa, comparative studies were carried out with the haemolytic complement fixation test (cft), indirect haemagglutination test and elisa with sera from patients with c ...1979113915
[chagas polyneuropathies].the results of a research realized in a small community in the countryside of the state of bahia, brazil, where there is high frequency of infection by trypanosoma cruzi. are reported. by evaluation the neurological clinical examination of 99 individuals it was observed that the most frequent finding on those 50 patients with positive sorology there was a diminution of deep reflexes. additionally, it was found that among the 20 patients in whom more than one altered deep reflex was present, in 1 ...1979115447
adoptive transfer of protection against trypanosoma cruzi with lymphocytes and macrophages.female c57bl/6j mice were infected with trypanosoma cruzi and subsequently given macrophages or lymphocytes from syngeneic donors which had recovered from the acute infection. mice which received immune peritoneal macrophages, splenic lymphocytes, or lymph node lymphocytes developed lower mean parasitemias and cumulative mortalities than did recipients of nonimmune cells. neither peritoneal lymphocytes nor splenic macrophages were protective, however. these studies indicate that splenic and lymp ...1979115790
urinary metabolites of 3a,4,5,6,7,7a-hexahydro-3-(1-methyl-5-nitro-1h-imidazol-2-yl)-1,2-benzisoxazole in the dog.the antiprotozoal drug 3a,4,5,6,7,7a-hexahydro-3-(1-methyl-5-nitro-1h-imidazol-2-yl)-1,2-benzisoxazole (i), which exhibits activity against trypanosomiasis, is also antibacterial in vivo. since the urine from a dog dosed with i showed a broader spectrum of antibacterial activity than i itself, metabolites from this urine were isolated and partially characterized. the metabolites were mono- and dihydroxy-substituted species with the hydroxyl groups on carbons 4--7 of the hexahydrobenzisoxazole ri ...1979115987
[trypanosoma cruzi: activity of mouse immune sera on the tyrpomastigote stage]. 1979116105
[pregnancy and trypanosoma cruzi parasitism]. 1979116106
protective immunisation of mice using cell surface glycoprotein from trypanosoma cruzi. 1979116128
[chagas' disease caused by blood transfusion in londrina, paraná. report of 2 acute cases treated with nifurtimox]. 2000116340
antibody-dependent killing of bloodstream forms of trypanosoma cruzi by human peripheral blood leukocytes.bloodstream forms of trypanosoma cruzi were found to be destroyed by human lymphoid cells, neutrophils, or eosinophils in the presence of specific antibodies. nonspecific immunoglobulins present in normal serum did not mediate the cytotoxic reaction. cells or antibody had no significant lytic effect on the parasite when tested separately and purified, non-activated human adherent cells were inactive antibody was present or not.1979116558
use of micro-elisa for quantitating antibody to trypanosoma cruzi and trypanosoma rangeli.of 229 residents of a panamanian village where both trypanosoma cruzi and trypanosoma rangeli are endemic, 52% had antibody to one or both species by the enzyme-linked immunosorbent assay (elisa), 26% were positive by complement fixation, 17% were positive by direct agglutination, and 32% were positive on the basis of clinical impression. although the sensitivity of elisa makes it the procedure of choice for sero-epidemiologic studies, there does appear to be some serologic cross-reactivity betw ...1979116559
[etiologic therapy of chagas' disease]. 1979116629
membrane-bound antibodies to bloodstream trypanosoma cruzi in mice: strain differences in susceptibility to complement-mediated lysis.the y, cl and other strains of trypanosoma cruzi display different morphological and immunological characteristics. such observations are here extended to the interaction of bloodstream forms of different strains of t. cruzi with components of the complement system. we demonstrate that the bloodstream forms of the y and b strains, but not those of the cl strain, are lysed by normal human serum. lysis is mediated by combined activities of the alternative and classical complement pathways. these a ...1979116784
trypanosoma cruzi: homocytotrophic antibody response in mice immunized with unrelated antigens. 1979116864
[electrocardiographic changes in young men with positive serologic tests for chagas disease resident in an endemic area]. 2006117275
immunochemical study of the lysates of the epimastigote forms of trypanosoma cruzi (chagas, 1909). 1979117521
[ecological aspects of south american trypanosomiasis. xiv. persistence and domicilization potential of wild triatomid populations in an intensive agricultural and cattle breeding region]. 1979117542
[an autopsy case of chronic chagas' disease (author's transl)]. 1979118226
[thyroid mass in chronic chagas disease]. 1979118495
chagas' disease. 1979118765
in vitro cellular immunity in chagas' disease.in vitro delayed hypersensitivity was studied in patients with either the chronic or the indeterminate phase of chagas' disease. normal healthy individuals were used as controls. the leucocyte migration inhibition test was applied using an antigen an extract of culture forms of t. cruzi or an extract of normal rat heart. the results showed that patients with the chronic phase of the disease reacted significantly with both antigens, whereas most patients with the indeterminate phase did not react ...1979118840
genetic control of the mechanisms of resistance against experimental trypanosoma cruzi infection. i: role of the macrophages in resistant and suceptible strains of mice. 1979119283
[epidemiology of chagas' disease in state of jalisco, republic of mexico]. 2006119326
carlos chagas (1879-1934): the discovery of trypanosoma cruzi and of american trypanosomiasis (foot-notes to the history of chagas's disease). 1979119337
dipetalogaster maximus (hemiptera, triatominae) for xenodiagnosis of patients with serologically detectable trypanosoma cruzi infection.in patients serologically positive for trypanosoma cruzi infection the three bug species/instar combinations used in xenodiagnosis showed third-instar dipetalogaster maximus to be more efficient in detecting circulating trypanosomes than the first instar of the same species which, in turn, is more sensitive than third-instar triatoma infestans. the sensitivity of the pool technique of faecal examination compared with individual dissection was investigated. four pool examinations (the product of ...1979119338
experimental trypanosoma cruzi infection in rhesus monkeys 111. electrocardiographic and histopathological findings.in five rhesus monkeys surviving 'peru strain' or 'strain 7' trypanosoma cruzi infection for six to eight years, positive xenodiagnosis results and high indirect fluorescent antibody titres (4096 - 65536) persisted until the animals were killed. abnormal electrocardiograph patterns in two monkeys (h and k) were possibly compatible with myocardial damage. histopathological changes attributable to t. cruzi infection were minor in four monkeys but severe in one (r). in this animal, infected with wh ...1979119339
attempts to produce megasyndrome in mice using stocks of trypanosoma cruzi associated with megaoesophagus in man.two stocks of trypanosoma cruzi isolated from patients with advanced megaoesophagus produced megastomach in chronically infected mice. the mice showed evidence of stomach dilatation and a delay in intestinal transit time. these findings are discussed in the light of the hypothesis that regional variations in t. cruzi determines mega formation in man.1979120044
an outbreak of acute chagas's disease in the são francisco valley region of bahia, brazil: triatomine vectors and animal reservoirs of trypanosoma cruzi.following reports of an unusually high incidence of acute chagas's disease and the appearance of large numbers of triatoma infestans in the southwestern region of the state of bahia, triatomine bugs (hemiptera: reduviidae) and domestic animals in one of the affected communities were surveyed and examined for infection with trypanosoma cruzi. triatoma infestans was prevalent in houses and was also found in peridomestic habitats. t. sordida and t. pseudomaculata occupied peridomestic and sylvatic ...1979120045
chagas' disease: trends in immunological research and prospects for immunoprophylaxis.acute infection with trypanosoma cruzi usually subsides spontaneously but the mortality rate encountered in individuals with the chronic infection is high. much evidence has accumulated in the last five years that autoimmunity plays an important role in the pathogenesis of the myocarditis that is common in the chronic phase. a negative relationship has been observed between the demonstrable parasitaemia and the presence of severe cardiac lesions. this myocarditis is characterized by lymphocytic ...1979120233
association between hbsag and race in a mixed population of northeastern brazil.the frequency of australian antigen carriers (hbsag) was studied in three samples from northeastern brazil. in sample a there were 1000 women from tsylla balbino maternity in salvador; in sample b there were 1,512 blood donors from salvador, and in sample c there were 930 blood donors from aracaju. in sample a, b and c the observed frequency of carriers (hbsag) was 1.20%, 1.58% and 2.04% respectively. these differences were not significant (x22 = 2.2; p greater than 0.25). there was no associati ...2002120564
immunization against experimental trypanosoma cruzi infection: inherent difficulties of a uniform comparative evaluation of antigenic preparations. 1979120626
the effect of lampit on trypanosoma cruzi in mice organs and in the bloodstream.mice infected with bloodstream forms of trypanosoma cruzi were treated with an active nitrofuran compound (nifurtimox, lampit). determination of the number of intracellular forms of t. cruzi in the liver and the spleen of control and lampit-treated mice showed that the drug induced a decrease in the number of parasites inside the cells. a decrease in the number of bloodstream forms was also observed. ultrastructural observations showed that lampit induces several alterations in t. cruzi, the mos ...1979120645
immune response in human chagas' disease i. lymphocyte blastogenesis in chagasic patients without evidence of cardiomyopathy. 1979120666
protection against trypanosoma cruzi using fecal forms of homologous parasites from juvenilized or normal vectors of chagas' disease and by passive transfer of immune blood. 1977120952
[transfer of humoral immunity to trypanosoma cruzi induced by heat in mice]. 1979120975
report on a field collection of dipetalogaster maximus (hemiptera triatominae) (uhler, 1894). 1979120977
muscle denervation in chronic chagas disease. 2004121145
[a review of the anatomo-clinical classification of chagas' disease]. 1979121233
[a study of 39 cases of acute chagas' disease in santa cruz, bolivia (author's transl)]. 2013121538
[reservoirs and wild vectors of trypanosoma cruzi. lxxiv. behavior of amastigotes and trypomastigotes of cultures of different t. cruzi strains incubated with normal human serum and inoculated into mice]. 1979121615
depression of blood monocytes chemotaxis in human chronic chagas disease. 1979121632
effects of parasitic infections in pregnant women.this review focuses on the parasitic diseases which occur frequently in the tropics and which affect pregnant women. clinical disease of the mother during pregnancy, vertical transmission of parasites and transplacental passage of soluble parasitic antigens are discussed in relation to their immunopathological significance for the fetus. the incidences of congenital malaria, african trypanosomiasis and chagas' disease are reviewed, together with vertical transmission of filarial infection, invol ...1979121975
a comparison of challenge with trypanosoma cruzi blood-stream trypomastigotes and metacyclic trypomastigotes from rhodnius prolixus in mice immunized with killed antigens.groups of cd-1 mice were immunized with vaccines prepared from freeze-thawed or ultra-sonicated epimastigotes, blood trypomastigotes, or "plasma antigen", of trypanosoma cruzi strains y, m1 and tulahuen. the mice were challenged by the injection of blood stream trypomastigotes obtained from mice, or of metacyclic trypomastigotes harvested from the rectum of rhodnius prolixus. both challenges induced virulent infections in control mice. blood-stream trypomastigotes killed mice more rapidly than t ...1979122124
[congenital chagas disease: anatomopathological findings in the placenta and umbilical cord]. 1979122217
[prevention of chagas disease]. 1979122241
[distribution of chagas disease in brazil]. 1979122243
[chagas disease: vector control activities in the state of são paulo, brazil]. 1979122244
[solid-phase radioimmunoassay for the study of the humoral response to trypanosoma cruzi].a solid-phase micro-radioimmunoassay (rie-s) test was adapted fore the study of the humoral immune response induced by trypanosoma cruzi infection. the solid phase, consisting of disposable polypropylene tubes, was sensitized by absorption of t. cruzi antigen. the procedure was performed by sequential adding to the solid-phase, the human serum under study and 131i anti-human immunoglobulin. then, the specific label binding was estimated. several factors which influence the test were studied. the ...1979122384
immunopathologic and morphologic studies of skeletal muscle in chagas' disease.skeletal muscle biopsies from 21 individuals infected with trypanosoma cruzi were studied my means of immunofluorescence, ultrastructural immunochemical, light and electron microscopic, and histochemical procedures. in 12 cases, definite morphologic alterations were found. these alterations were coincident with the presence of circulating antibodies against the plasma membrane of striated muscle fibers and endothelial cells (evi antibodies). in almost all cases the lesions also presented auto ...1975125546
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