Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| molecular cloning of wheat dihydrodipicolinate synthase. | four forms of dihydrodipicolinate synthase (dhdps), which catalyzes the first reaction in the lysine-specific biosynthetic pathway in higher plants, were purified to homogeneity from a suspension culture of wheat (triticum aestivum). these polypeptides have similar n-terminal amino acid sequences. two different cdna clones were isolated by screening a wheat cdna library with oligonucleotide probes based on these amino acid sequences. the predicted amino acid sequences indicate that both clones e ... | 1990 | 2211639 |
| growth dynamics of wheat (triticum aestivum l.) exposed to sulfur dioxide pollution. | 1990 | 2257336 | |
| electron microscopic demonstration of lectin binding sites in the taste buds of the european catfish silurus glanis (teleostei). | taste buds in the european catfish silurus glanis were examined with electron microscopic lectin histochemistry. for detection of carbohydrate residues in sensory cells and adjacent epithelial cells, gold-, ferritin- and biotin-labeled lectins were used. a post-embedding procedure carried out on tissue sections embedded in lr-white was applied to differentiate between the sensory cells: the lectins from helix pomatia (hpa) and triticum vulgare (wga) bound to n-acetyl-galactosamine and to n-acety ... | 1990 | 2279957 |
| 500-mhz proton nmr evidence for two solution structures of the common arm base-paired segment of wheat germ 5s ribosomal rna. | the base-pair protons of the common arm duplex fragment of wheat germ (triticum aestivum) ribosomal 5s rna have been identified and assigned by means of 500-mhz proton nmr spectroscopy. the two previously reported extra base pairs within the fragment [li et al. (1987) biochemistry 26, 1578-1585] are now explained by the presence of two distinct solution structures of the common arm fragment (and its corresponding base-paired segment in intact 5s rrna). the present conclusions are supported by on ... | 1990 | 2331462 |
| wheat germ 5s ribosomal rna common arm fragment conformations observed by 1h and 31p nuclear magnetic resonance spectroscopy. | the nonexchangeable protons of the common arm fragment of wheat germ (triticum aestivum) ribosomal 5s rna have been observed by means of high-resolution 500-mhz 1h nmr spectroscopy in d2o solution. although nmr studies on the exchangeable protons support the presence of two distinct solution structures of the common arm fragment (and of the same base-paired segment in intact 5s rrna), only a single conformation is manifested in the 1h nmr behavior of all of the h6 and h5 pyrimidine and most of t ... | 1990 | 2331463 |
| nucleotide sequences of two nuclear trna(tyr) genes from triticum aestivum. | 1990 | 2336367 | |
| characterization of amyloplastic phosphohexose isomerase from immature wheat (triticum aestivum l.) endosperm. | phosphohexose isomerase from amyloplasts of immature wheat endosperm was purified 133-fold. the enzyme had a molecular weight of 130 kda and maximum activity at ph 8.6. it showed normal hyperbolic kinetics for both fructose-6-p and glucose-6-p with km of 0.12 mm and 0.44 mm, respectively. ph had a great influence on km for fructose-6-p. using glucose-6-p as the substrate, the equilibrium was reached at 23% fructose-6-p and 77% glucose-6-p and an equilibrium constant of about 3.0. the delta f cal ... | 1990 | 2341161 |
| glycoconjugate histochemistry in the glycocalyx of guinea pig middle ear mucosa. | an attempt was made to determine the composition of oligosaccharide side chains in the apical cell surface glycocalices of the middle ear epithelium. this glycoconjugate is located on the innermost luminal surface, but its function is still unknown. the lectins evaluated included triticum vulgare, canavalia ensiformis, maclura pomifera, arachis hypogaea, ricinus communis i (rca-i), and limax flavus. the staining results obtained with the six different lectin probes in the lowicryl k4m-embedded g ... | 1990 | 2393562 |
| preparative isoelectric focusing of reduced wheat gluten proteins. | proteins extracted from gluten of the bread wheat cultivar fiorello 2 in the presence of 2-mercaptoethanol or dithiothreitol were separated by isoelectric focusing in a free solution in a ph 3-10 gradient containing 50% v/v 1-propanol or urea. the collected fractions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in 10% gels (high and medium molecular weight glutenin subunits) and 16% gels (low molecular weight gliadins). the isoelectric focusing pattern of gluten pol ... | 1990 | 2394208 |
| a major endothelial plasmalemmal sialoglycoprotein, gp60, is immunologically related to glycophorin. | glycophorins, the major sialoglycoproteins of red blood cells in many species, are generally considered to be specific to erythroid cells. using polyclonal antibodies directed against mouse glycophorin (alpha gp), we have identified a glycoprotein antigenically related to glycophorin on the surface of bovine and rat cultured endothelial cells. immunoblotting with alpha gp identified a single 60-kda polypeptide on transfers of sds/polyacrylamide gels of solubilized confluent endothelial monolayer ... | 1990 | 2395877 |
| lectin-binding ability of immunoglobulin e and its participation in triggering of mast cells. | eleven purified lectins were compared in their ige-precipitating, mast-cell-degranulating and histamine-releasing activity and in eliciting skin allergic reactions in atopic or normal people. a significantly positive relationship could be proved between their ige-precipitating activity and degranulation of ige-sensitized rat mast cells. a similar and often significant relation was observed in skin reactions and histamine released from human leukocytes. soybean lectin reacted with concanavalin a ... | 1985 | 2408980 |
| determination of amylase activity in serum by using a wheat germ inhibitor with the du pont aca. | a rapid procedure for determining salivary- and pancreatic-type amylase (ec 3.2.1.1) in serum by incorporating a wheat germ inhibitor (from triticum aestivum) was developed for the du pont aca iv analyzer. under optimal assay conditions, activities of salivary and pancreatic amylase were inhibited by 93% and 19%, respectively. the 95% central reference interval for the percentage of inhibition of serum amylase was 38-84%. patients with acute pancreatitis showed less than 26% inhibition of amylas ... | 1986 | 2426009 |
| a reverse transcriptase-like activity of wheat (triticum aestivum) embryo microsomal fraction. | a reverse transcriptase-like activity was isolated from germinating wheat (triticum aestivum) embryos. the activity was found to be associated with a microsomal fraction (70,000 g pellet) of the embryo homogenate. the microsome-associated enzyme prefers homologous polyadenylated rna to any other polynucleotides as template and requires all four deoxyribonucleoside triphosphates for maximal activity. the reaction product appears in the incubation mixture in the form of an rna-dna hybrid, which ca ... | 1987 | 2449165 |
| detection of fungi in tissue sections by lectin histochemistry. | a battery of 21 fluoresceinated lectins was tested on sections of paraffin-embedded human tissues to determine which lectin could be used in the microscopic diagnosis of fungal diseases. three lectins, from canavalia ensiformis (concanavalin), triticum vulgare (wheat germ a), and phytolacca americana (pokeweed [pwm]), were found to react with fungal pathogens commonly encountered in nosocomial infections. best results were obtained with pwm and succinylated wheat germ a, which, due to their weak ... | 1988 | 2454619 |
| the transcription and translation in vitro of individual cereal storage-protein genes from wheat (triticum aestivum, cv. chinese spring). evidence for translocation of the translation products and disulphide-bond formation. | genes coding for the high-mr ['high-molecular-weight' (hmw)] glutenin subunit 12 and for a gamma-gliadin from wheat (triticum aestivum, cv. chinese spring) were subcloned into transcription-translation vectors. in each case transcription in vitro yielded a rna transcript which when added to a rabbit reticulocyte cell-free translation system directed the synthesis of a polypeptide of appropriate mr by sds/polyacrylamide-gel electrophoresis (sds/page). when dog pancreatic microsomal vesicles were ... | 1988 | 2461700 |
| cytochemical localization of carbohydrates in intercalated duct and acinar cells of mouse parotid gland. | the glycoconjugate composition of mouse intercalated duct and acinar cells of parotid gland has been compared. mucins containing 1,2-glycols were demonstrated by the tannic acid-uranyl acetate technique. hexose residues of glycoconjugates were identified using ferritin conjugated with canavalia ensiformis agglutinin (con a), triticum vulgare or wheat germ agglutinin (wga), ricinus communis i agglutinin (rca-i), phaseolus vulgaris agglutinin (pha-e) and arachis hypogaea agglutinin (pna). whereas ... | 1989 | 2470700 |
| regional distribution of glycoconjugates in normal, transitional and neoplastic human colonic mucosa. a histochemical study using lectins. | regional distribution of glycoconjugates in normal and neoplastic colonic mucosa was studied by means of eight lectins: dolichos biflorus (dba), glycine max (sba), triticum vulgare (wga), arachis hypogaea (pna), griffonia simplicifolia-i (gs-i), canavalia ensiformis (con a), limax flavus (lfa), and ulex europaeus-i (uea-i). the lectin binding patterns were examined in 40 normal colonic mucosa (nm) (12 proximal (p) and 28 distal (d], 38 carcinomas (15 p and 23 d), and 31 transitional mucosa (tm) ... | 1989 | 2505446 |
| molecular analysis of an alcohol dehydrogenase (adh) gene from chromosome 1 of wheat. | we have cloned and determined the nucleotide sequence of a gene encoding alcohol dehydrogenase (adh) from triticum aestivum cv. millewa. southern analysis using cv. chinese spring nullisomic-tetrasomic and ditelosomic lines established that the cloned gene mapped to the long arm of chromosome 1a and does not correspond to any previously identified wheat adh locus. southern analysis also provided evidence for triplicate copies of this adh gene on the homoeologous group 1 chromosomes, while northe ... | 1989 | 2545522 |
| transient gene expression in aleurone protoplasts isolated from developing caryopses of barley and wheat. | methods have been developed for the isolation of aleurone protoplasts from developing caryopses of hordeum vulgare and triticum aestivum in order to study transient expression of introduced genes. chimaeric gene constructs were introduced into aleurone protoplasts by polyethylene glycol (peg). transient expression directed by the 35s promoter from cauliflower mosaic virus (camv) of the reporter gene encoding chloramphenicol acetyl transferase (cat) was detected in aleurone protoplasts from devel ... | 1989 | 2562757 |
| nucleotide sequences of the two high-molecular-weight glutenin genes from the d-genome of a hexaploid bread wheat, triticum aestivum l. cv cheyenne. | 1989 | 2563152 | |
| conformational differences between two wheat (triticum aestivum) 'high-molecular-weight' glutenin subunits are due to a short region containing six amino acid differences. | 'high-molecular-weight' (hmw, high-mr) glutenin subunits are protein constituents of wheat (triticum aestivum) seeds and are responsible in part for the viscoelasticity of the dough used to make bread. two subunits, numbered 10 and 12, are the products of allelic genes. their amino acid sequences have been derived from the nucleic acid sequences of the respective genes. subunit 10 has fewer amino acids than subunit 12, but migrates more slowly on sds/page (polyacrylamide-gel electrophoresis). th ... | 1989 | 2597130 |
| psbg is not a photosystem two gene but may be an ndh gene. | a gene of the chloroplast genome has been designated the psbg gene on the basis that in maize the gene product is a 24-kda polypeptide of photosystem two (ps2) (steinmetz, a. a., castroviejo, m., sayre, r. t., and bogorad, l. (1986) j. biol. chem. 261, 2485-2488). we have located and sequenced the equivalent gene in wheat (triticum aestivum) and have raised specific antibodies to the gene product following its expression in escherichia coli as a beta-galactosidase fusion protein. using these ant ... | 1989 | 2668282 |
| transport of proteins into chloroplasts. import and maturation of precursors to the 33-, 23-, and 16-kda proteins of the photosynthetic oxygen-evolving complex. | the 33-, 23-, and 16-kda proteins of the photosynthetic oxygen-evolving complex are synthesized as precursors in the cytoplasm and transported into the thylakoid lumen of higher plant chloroplasts. in this report we have analyzed the import and maturation of these precursors, using reconstituted protein import assays and partially purified preparations of the processing peptidases involved. precursors of the 33- and 23-kda proteins from spinacia and triticum aestivum are processed by a stromal p ... | 2008 | 2684958 |
| lectin-binding sites in neoplastic and non-neoplastic colonic mucosa of 1,2-dimethylhydrazine-treated rats. | qualitative changes of glycoconjugates in luminal surface and goblet cell mucin from colon mucosa of 1,2-dimethylhydrazine (dmh)-treated rats were studied. eight fluoresceinated lectins were used: dolichos biflorus (dba), glycine max (sba), triticum vulgare (wga), limax flavus (lfa), arachis hypogaea (pna), griffonia simplicifolia-i (gs-i), ulex europaeus-i (uea-i) and canavalia ensiformis (con a). the lectin-binding patterns were studied in tumors arising in proximal and distal portions of the ... | 1989 | 2689774 |
| arabinoxylans from rye and wheat seed that interact with ice. | arabinoxylans that interfere with growth of ice crystals have been purified from rye (secale cereale l., rosen) and two varieties of wheat (triticum aestivum l., genesee and hillsdale) seed. the most active polysaccharide from each seed type was homogeneous in the sense that all the molecules were in the same size range, they contained the same sugar residues, and they reacted similarly in chemical characterization experiments. structural studies showed that the polysaccharides consist of a xyla ... | 1989 | 2743349 |
| a nuclear trna(ugaser) gene from the wheat triticum vulgare var. aria. | the nucleotide sequence of a wheat nuclear trna(ugaser) gene from triticum vulgare var. aria has been determined. it has a typical intragenic promoter with boxa and boxb elements and a putative termination signal 12 nucleotides downstream from the last trna-coding nucleotide. the region upstream from the coding segment contains a g + c-rich sequence with a symmetrical element. the sequence described is the first nuclear trna gene from a monocotyledonous plant. | 1989 | 2744486 |
| lectin binding sites in normal rat ovary and enu-induced sertoli cell tumors of the ovaries. | a panel of seven fluorescein isothiocyanate (fitc) labeled lectins were used to study the distribution of specific binding sites in histologic sections of rat ovaries and enu-induced sertoli cell tumors (sct) of the ovaries. ten sct and 5 normal ovaries derived from berlin druckey iv (bd-iv) rats were examined by fitc lectins. the tissues examined were fixed in 10% buffered formalin and embedded in paraffin blocks. in normal ovaries, lectin binding sites were more uniform, ordered and consistent ... | 1989 | 2764514 |
| new dimeric inhibitor of heterologous alpha-amylases encoded by a duplicated gene in the short arm of chromosome 3b of wheat (triticum aestivum l.). | a new wheat dimeric alpha-amylase inhibitor, designated wdai-3, has been characterized. wdai-3 is a homodimeric protein active against alpha-amylase from human saliva and from the insect tenebrio molitor, but inactive against that from pig pancreas or against trypsin. its n-terminal amino acid sequence is closer to those of the wheat dimeric inhibitors 0.19 and 0.53 (89-91% identical positions in 44 residues) than to that of the monomeric 0.28 inhibitor (69% identical positions). iha-b1-2, the g ... | 1989 | 2787745 |
| linked sucrose synthase genes in group-7 chromosomes in hexaploid wheat (triticum aestivum l.). | a cdna library from developing wheat endosperm was screened for sucrose-synthase clones using a maize cdna probe corresponding to the sh1 locus under non-stringent conditions. five positive clones were isolated and initially classified into two types on the basis of their relative ability to hybridize with the probe and of their partial restriction maps. determination of the nucleotide sequences indicated homology between the two types of wheat clones, with type 1 showing higher homology to the ... | 1988 | 2838390 |
| enzymic capacities of amyloplasts from wheat (triticum aestivum) endosperm. | lysates of protoplasts from the endosperm of developing grains of wheat (triticum aestivum) were fractionated on density gradients of nycodenz to give amyloplasts. enzyme distribution on the gradients suggested that: (i) starch synthase and adp-glucose pyrophosphorylase are confined to the amyloplasts; (ii) pyrophosphate: fructose-6-phosphate 1-phosphotransferase and udp-glucose pyrophosphorylase are confined to the cytosol; (iii) a significant proportion (23-45%) of each glycolytic enzyme, from ... | 2010 | 2849412 |
| commodities into food. | it is not surprising that past predictions of the extent of adoption of new technology by the food and agricultural industries have only been partly realized. this is a result of the difficulty of forecasting the take-up of technology that is capable of being transferred from other industries, for example, process control methods and the advent of new packaging materials. most technology that is adopted is normally incremental over the existing technology and forecasting of this type is best don ... | 0 | 2865759 |
| effects of supplemental silage on forage intake and utilization by steers grazing wheat pasture or bermudagrass. | seven trials were conducted to determine the effects of increasing amounts of silage (corn, wheat or sorghum) on forage intake, gastrointestinal tract fill, fecal output and ruminal flow and degradation of forage by cattle grazing wheat pasture or bermudagrass. in each of 3 yr, 24 steers grazed a common wheat or bermudagrass pasture and were randomly allocated to four treatments (0, .35, .70 or 1.05 kg silage dm.100 kg body weight-1.d-1). intake and ruminal flow of forage were measured by feedin ... | 1989 | 2925545 |
| em polypeptide and its messenger rna levels are modulated by abscisic acid during embryogenesis in wheat. | the effect of abscisic acid (aba) on the expression of the 'early-methionine-labeled' (em) polypeptide was examined in cultured, immature wheat (triticum aestivum, l.) embryos and in developing embryos in planta. a complementary dna (cdna) library was constructed from poly(a)-rich rna from immature embryos cultured in the presence of aba. aba-enhanced sequences were first identified by differential colony-blot hybridization, and then verified using rna slot-blot analysis. dot-blot hybridization ... | 1985 | 2932332 |
| influence of edta on the atpase activities of plasma membranes from winter wheat roots. | plasma membrane vesicles were purified from roots of winter wheat seedlings (triticum aestivum l. cv. marton-vásári-8) by aqueous polymer two-phase partitioning. the atpase activity of vesicles in the presence of ca was independent of but in the presence of mg depended on the edta concentration in the atpase assay. the potassium stimulation and vanadate inhibition of the mgatpase also depended on the edta concentration. consequences of these results are briefly discussed. | 1988 | 2976563 |
| the nucleotide sequence of a hmw glutenin subunit gene located on chromosome 1a of wheat (triticum aestivum l.). | a cloned 8.2 kb ecori fragment has been isolated from a genomic library of dna derived from triticum aestivum l. cv. cheyenne. this fragment contains sequences related to the high molecular weight (hmw) subunits of glutenin, proteins considered to be important in determining the elastic properties of gluten. the cloned hmw subunit gene appears to be derived from chromosome 1a. the nucleotide sequence of this gene has provided new information on the structure and evolution of the hmw subunits. ho ... | 1985 | 2997729 |
| lectin histochemistry applied to human nerves. | histologic sections of normal and pathologically altered human peripheral nerves were stained with a battery of 20 fluorescein isothiocyanate-labeled lectins to determine whether these histochemical reagents could be used to identify peripheral nerve injury. eight plant lectins--from canavalia ensiformis, lens culinaris, triticum vulgare (wheat germ), types e4 and l4 from phaseolus vulgaris, types i and ii from ricinus communis, and wistaria floribunda--were found to bind to normal and pathologi ... | 1986 | 3015069 |
| structure of wheat gamma-gliadin genes. | we have cloned and sequenced two linked members of the wheat (triticum aestivum) gliadin multigene family. one gene encodes a gamma-gliadin which is 292 amino acids (aa) long. s1 mapping indicates that this gene is transcriptionally active. the second gene, which is only marginally active by s1 mapping, is closely related to the first gene. moreover, it is probably incapable of encoding a full-length gamma-gliadin due to the presence of two premature in-frame stop codons. neither gene contains i ... | 1986 | 3017812 |
| relative nutritional availability to rats of selenium in finnish spring wheat (triticum aestivum l.) fertilized or sprayed with sodium selenate and in an american winter bread wheat naturally high in se. | a finnish national programme to fertilize crops with sodium selenate led us to compare the nutritional availability to rats of selenium in two finnish spring wheats (triticum aestivum l.), either fertilized or sprayed with sodium selenate, with that in an american winter bread wheat naturally high in se. weanling male rats were given a se-deficient torula yeast diet for 4 weeks followed by either continued depletion or repletion for 4 weeks with graded levels of se as sodium selenite (standard) ... | 1987 | 3036197 |
| temperature and the cell cycle. | during the period between successive divisions, a cell traverses three stages of interphase: g1 (pre-synthetic interphase), s-phase (dna synthetic interphase) and g2 (post-synthetic interphase). the time taken for all cells in a meristem to divide (the cell doubling time (cdt] decreases in response to an increase in temperature. for example, the cdt in root meristems of zea mays decreases 21-fold as the temperature is increased from 3 to 25 degrees c. whether all phases of the cell cycle alter p ... | 1988 | 3077857 |
| 500-mhz proton homonuclear overhauser evidence for additional base pair in the common arm of eukaryotic ribosomal 5s rna: wheat germ. | a "common-arm" fragment from wheat germ (triticum aestivum) 5s rna has been produced by enzymatic cleavage with rnase t1 and sequenced via autoradiography of electrophoresis gels for the end-labeled fragments obtained by further rnase t1 partial digestion. the existence, base pair composition, and base pair sequence of the common arm are demonstrated for the first time by means of proton 500-mhz nuclear magnetic resonance. from mg2+ titration, temperature variation, ring current calculations, se ... | 1987 | 3109468 |
| characterization and quantification of low molecular weight glutenins in durum wheats. | durum wheat proteins have been considered as a model because of the very clear-cut relationship previously evidenced between the electrophoretic type '42' or '45' of the components that are coded by the gli-b1 chromosome locus and the intrinsic quality (gluten viscoelasticity) of cultivars. the proteins from 4 cultivars were subjected to sequential extraction and separated into five groups, respectively, in: nacl, etoh (gliadins-i), etoh + mercaptoethanol (me) (gliadins-ii), acoh + me (glutenins ... | 2016 | 3120804 |
| biochemical data bearing on the relationship between the genome of triticum urartu and the a and b genomes of the polyploid wheats. | to determine whether the triticum urartu genome is more closely related to the a or b genome of the polyploid wheats, the amino acid sequence of its purothionin was compared to the amino acid sequences of the purothionins in triticum monococcum, triticum turgidum, and triticum aestivum. the residue sequence of the purothionin from t. urartu differs by five and six amino acid substitutions respectively from the alpha 1 and alpha 2 forms coded for by genes in the b and d genomes, and is identical ... | 1988 | 3209062 |
| hydrophobic-cluster analysis of plant protein sequences. a domain homology between storage and lipid-transfer proteins. | hydrophobic-cluster analysis was used to characterize a conserved domain located near the c-terminal amino acid sequence of wheat (triticum aestivum) storage proteins. this domain was transformed into a linear template for a global search for similarities in over 5200 protein sequences. in addition to proteins that had already been found to exhibit homology to wheat storage proteins, a previously unreported homology was found with non-specific lipid-transfer proteins from castor bean (ricinus co ... | 1988 | 3214430 |
| polymorphism and inheritance of gliadin components controlled by chromosome 6a of spring durum wheat. | the gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (al-lactate, ph 3.1) and two-dimensional (first dimension, al-lactate, ph 3.1; second dimension, sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. analysis of hybrids has shown that all components of the alpha zone of gliadin spectra are inherited together as blocks and are, probably, coded for by a cluster of tightly linked genes located on chromosome 6a. fourteen variants of gliadin blocks ... | 1988 | 3242490 |
| genetic control of nadh dehydrogenase-1 and aromatic alcohol dehydrogenase-2 in hexaploid wheat. | the genetic control of nadh dehydrogenase-1 (ndh-1) and aromatic alcohol dehydrogenase-2 (aadh-2) was investigated in triticum aestivum cv. chinese spring. evidence was obtained that ndh-1 is active as a monomer and is encoded by genes located in the p arms of the homoeologous group 4 chromosomes. the ndh-1 gene loci located in 4ap, 4bp, and 4dp were designated ndh-a1, ndh-b1, and ndh-d1, respectively. aadh-a2 was previously reported to be located in 6aq; in this study, aadh-b2 and aadh-d2 were ... | 1987 | 3329906 |
| scanning electron microscope studies of agrobacterium tumefaciens attachment to zea mays, gladiolus sp., and triticum aestivum. | scanning electron microscope studies demonstrated that cells of agrobacterium tumefaciens strains attach to cells on the cut surfaces of corn and wheat seedlings and to gladiolus disks. bacterial cells attached to these monocots in the same manner as they attached to the dicots tested. of the strains tested, a66 and t37 covered more of the cut surfaces of these monocots in a nonrandom fashion than did cells of other isolates. these bacteria attached to cells of intact monocotyledonous plants and ... | 1988 | 3360748 |
| prevention by mannan and other sugars of in vitro damage of rat fetal small intestine induced by cereal prolamin peptides toxic for human celiac intestine. | peptic-tryptic-cotazym and peptic-tryptic digests were obtained, simulating in vivo protein digestion, from pure "bread" wheat gliadins and from rye, barley, and oats prolamine and tested on small intestine cultures from fetal rats. when tested at a concentration of 0.1 mg of peptides/ml of culture medium the peptic-tryptic-cotazym and peptic-tryptic digests of gliadin and prolamines were very active in slowing in vitro development of fetal rat intestine and in increasing the occurrence and seve ... | 1987 | 3431955 |
| comparisons of proteins and glycoproteins in neuronal plasma membranes, axolemma, synaptic membranes, and oligodendroglial plasma membranes. | neuronal membranes are unique in that they consist of several functionally distinct segments: the perikaryal plasma membrane, the axolemma, the synaptic membrane, and the dendritic membrane. methods are now available to isolate the first three types of membranes as well as to isolate oligodendroglial plasma membranes. the protein and glycoprotein compositions for each set of membranes were analyzed by silver staining after separation by sds polyacrylamide gradient gel electrophoresis and by radi ... | 1987 | 3599099 |
| a quantitative fluorescence enzyme immunoassay for plant cytokinins. | an enzyme-linked immunosorbent assay (elisa) which used 4-methylumbelliferyl phosphate as an enzyme substrate was used to quantify two plant cytokinins. this assay detected as little as 0.03 pmol (approximately 10 pg) of cytokinin in microplate wells coated with a cytokinin-ovalbumin conjugate. the method measured competition between free cytokinin and the bound conjugate for reaction with monoclonal anticytokinin antibodies and used a standard curve prepared by use of known amounts of free cyto ... | 1987 | 3605594 |
| genetic control of the mitochondrial form of superoxide dismutase in hexaploid wheat. | extracts of mature grains of a large number of aneuploid derivatives of triticum aestivum cv. chinese spring and of the members of five wheat-alien chromosome addition series were subjected to isoelectric focusing in polyacrylamide gels in order to study the genetic control of superoxide dismutase (sod). evidence was obtained that homologous structural genes for the mitochondrial form of sod are located in the long arms of the homologous group 2 chromosomes of chinese spring and in chromosome 2r ... | 1986 | 3741368 |
| stimulation of cell division in mouse fibroblast line 3t3 by an extract derived from triticum vulgare. | the ability of an extract derived from triticum vulgare, the common wheat plant, to stimulate cellular proliferation of mouse 3t3 fibroblast cells was investigated. cellular response to triticum extract (te) was most evident in sparse cultures made quiescent by growing cells on low concentrations (0.6%) of calf serum. the growth-promoting activity in the extract was lost after dialysis but was resistant to heat treatment and digestion with trypsin or chymotrypsin, suggesting a low-molecular-weig ... | 1986 | 3744720 |
| in vitro activation of adenylate cyclase of atrophic celiac intestinal mucosa by wheat gliadin-derived peptides. | in order to demonstrate that gliadin peptides may interact with cell membranes of celiac small intestinal mucosa, the capacity of these peptides to activate the cell membrane enzyme adenylate cyclase was tested. the addition of peptides from bread wheat purified a-gliadin and whole gliadin (proteins that are toxic for celiac patients) enhanced the adenylate cyclase activity of crude cell membrane preparations obtained from atrophic small intestinal mucosa of celiac patients. no activation of ade ... | 1986 | 3945514 |
| effects of coal-fired thermal power plant discharges on agricultural soil and crop plants. | the physicochemical properties of the upstream and downstream waters from the upper ganga canal, discharged cooling tower water, machine washings, and scrubber and bottom ash effluents of a 530 mw kasimpur coal-fired thermal power plant have been determined, and their effects directly on fertile soil and indirectly on pea (pisum sativam) and wheat (triticum aestivum) crops have also been studied. the effluents were found to be alkaline in nature. the scrubber and bottom ash effluent was found to ... | 2014 | 3956466 |
| homology between legumin-like polypeptides from cereals and pea. | the presence of legumin-like constituents within the globulin fractions of wheat (triticum aestivum), rye (secale cereale) and corn (maize, zea mays) was demonstrated. two-dimensional analysis of wheat globulins in the presence and absence of a reducing agent revealed the existence of reducible approximately 60 kda polypeptides. western-blot analysis with 125i-labelled antibodies raised against the oat (avena sativa) 12s globulin holoprotein or its alpha-subunits demonstrated, firstly, the immun ... | 1985 | 3985947 |
| the amino acid sequence of wheat histone h2a(1). a core histone with a c-terminal extension. | the complete amino acid sequence (145 residues) of histone variant h2a(1) from wheat germ triticum aestivum cultivar t4 has been established from edman degradation of large overlapping fragments. the sequence of histone variant h2a(1) differs from the homologous calf histone in 61 amino acid positions. these differences include an extension of h2a(1) by 19 amino acids at its carboxyl end. | 1985 | 4018096 |
| effect of triticum vulgare extract on dna synthesis of mammalian cells. | 1985 | 4034741 | |
| microtubules and root protophloem ontogeny in wheat. | protophloem ontogeny in roots of triticum aestivum has been investigated ultrastructurally. each protophloem pole consists of three cells, a protophloem sieve element and two companion cells, all originating from a single precursor cell usually having a pentahedral shape. this protophloem mother cell (pmc) undergoes two successive asymmetrical divisions: the first one gives rise to a smaller cell that will differentiate into a companion cell, and a larger one that divides again asymmetrically yi ... | 1985 | 4044669 |
| interlocked bivalents in reconstructed metaphase i cells of bread wheat. | complete reconstructions of all the bivalents were made from electron micrographs of serial sections through six pollen mother cells at metaphase i of meiosis in triticum aestivum (hexaploid bread wheat). at least two of these metaphases contained interlocked pairs of bivalents. in one, two ring bivalents were interlocked, while in another a rod bivalent ran through the centre of a ring bivalent. two other groups of bivalents were too closely appressed to allow separation into individual bivalen ... | 1985 | 4044683 |
| cytotoxic effects of wheat gliadin-derived peptides. | the peptic-tryptic-cotazym (ptc) digest, obtained from bread wheat gliadin by simulating in vivo protein digestion, was more active than the ptc-digest of durum wheat gliadin in reversibly inhibiting hep-2 cell proliferation and in increasing cellular acid phosphatase. colony-forming ability of the cells was not affected by treatment with both bread or durum wheat gliadin peptides. the peptic-tryptic (pt) digest of bread wheat gliadin also showed agglutinating activity of hep-2 cells. | 1985 | 4071551 |
| determination of organic acids in seven wheat varieties by capillary gas chromatography. | fresh wheat tops were extracted with acidic 90% ethanol, and the ethanol was evaporated and a portion of the aqueous residue loaded onto deae-sephadex. organic acids were eluted with pyridinium formate and then lyophilized and the dried residue was derivatized with 1% trimethylchlorosilane in bis(trimethylsilyl)trifluoroacetamide. the acids were then quantitatively determined using capillary gas chromatography and identified using capillary gas chromatography-mass spectrometry. the acidic ethano ... | 1985 | 4073499 |
| further studies on a specific basic protein from bread wheat. | 2014 | 4202861 | |
| [genetic specificity of beta amylase in triticum aestivum: existence of 5 types of zymograms]. | 1974 | 4211447 | |
| [on some pecuiarities of action of 2 radioprotective substances, sodium diethyldithiocarbamate (na dedt) and 2-aminoethylisothiouronium (aet) on respiration in radicular meristems of triticum vulgare]. | 1968 | 4236641 | |
| iodine binding capacity of wheat starches & characteristics of wheat (triticum aestivum). | 2014 | 4248656 | |
| production of initial material for the breeding of short-stemmed varieties of soft wheat with enhanced technological qualities of the grain by means of crossing soft wheat (triticum aestivum l.) with hard wheat (t. durum desf.). | 1974 | 4453839 | |
| the use of double-monotelodisomics to identify translocations in triticum aestivum. | 1974 | 4463177 | |
| variation at two loci affecting homoeologous meiotic chromosome pairing in triticum aestivum x aegilops mutica hydrids. | 1972 | 4500460 | |
| differential localization of cell surface and secretory components in rat intestinal epithelium by use of lectins. | sections through various levels of small intestine from adult male rats were examined by fluorescence microscopy after treatment with fluorescein isothiocyanate-labeled lectins from dolichos biflorus, lotus tetragonolobus, ricinus communis, and triticum vulgare (wheat germ). the latter three lectins reacted with the microvillar portion of the epithelial cells lining the crypts and villi in sections of intestine adjacent to the pylorus. this pattern of reactivity was sharply altered along the fir ... | 1974 | 4609988 |
| the organization and polarity of pollen mother cells of triticum aestivum. | 1972 | 4648502 | |
| effects of patulin and method of application on growth stages of wheat. | when a single, 100-mug/ml application of patulin, produced by penicillium urticae bainier, was applied to growth stages 7, 9, 10, and 10.1 (feekes scale) of lee spring wheat (triticum aestivum l.), decreases in internodal elongation, floret number, seed weight, and seed number were observed. yields were reduced according to the proximity of application prior to heading. application of patulin to the soil in crystalline form and dissolved in aqueous solution were also investigated, and the soluti ... | 1973 | 4699215 |
| a survey of the multiple forms of invertase in the leaves of winter wheat, triticum aestivum l. emend thell. ssp. vulgare. | 1973 | 4750765 | |
| content of sr90, ca, and strontium units in the grain of wheat hybrids obtained by crossing triticum aestivum times triticum dicoccum. | 1973 | 4783216 | |
| cytogenetic study of the interspecific wheat hybrid triticum aestivum x triticum dicoccum. i. characteristics of the original form and f1 hybrids. | 1974 | 4816384 | |
| triploidy and aneuploidy in virus infected wheat, triticum aestivum. | 1974 | 4845180 | |
| the organization of the nucleolus in meristematic plant cells. a cytochemical study. | the architecture of the nucleolus in allium porum and triticum vulgare meristematic cells has been investigated by means of digestions with various enzymes. after staining with azure b at ph4, plant nucleoli exhibit lighter regions which, under electron microscopy, correspond to the fibrillar zones characterizing these organelles. evidence is presented indicating that these latter zones contain coarse convoluted filaments quite similar to the loops first demonstrated by la cour (24) and which ar ... | 1969 | 4885477 |
| factors influencing agnotobiotic cultures of the rumen ciliate, entodinium simplex. | the nutrition of entodinium simplex was studied, with foliage of bluegrass (poa pratense), perennial ryegrass (lolium perenne), and grains of wheat (triticum vulgare) as substrates in agnotobiotic cultures. entodinium grew poorly when the substrates were autoclaved; better growth was obtained when the substrates were sterilized with ethylene oxide vapor. the concentration of ethylene oxide and the amount of moisture influenced the sterility and nutritional adequacy of the treated substrate. auto ... | 1968 | 4969666 |
| the effect of chromosomes 5b, 5d, and 5a on chromosomal pairing in triticum aestivum. | 1966 | 5227663 | |
| somatic association in triticum aestivum. | 1966 | 5230145 | |
| genetic regulation of meiotic chromosome pairing by chromosome 3d of triticum aestivum. | 1971 | 5283628 | |
| [determination of sitosterol palmitate in different varieties of triticum aestivum and triticum durum]. | 1969 | 5398532 | |
| the timing of growth promotion and conversion to indole-3-acetic acid for auxin precursors. | using a high resolution continuous recording technique, the length of the latent period preceding the growth response to two suspected precursors of indole-3-acetic acid (iaa) indole-3-acetonitrile (ian), and indole-3-ethanol was studied in corn (zea mays l.), wheat (triticum aestivum l.), and peas (pisum sativum l.). the timing of the conversion in vivo of these presumed precursors to iaa was also examined. in wheat the rate of conversion of ian to iaa is rapid, and the latent period in the gro ... | 1970 | 5423464 |
| buoyant density studies of chloroplast and nuclear deoxyribonucleic acid from control and 3-amino-1,2,4-triazole-treated wheat seedlings, triticum vulgare. | the isolation of chloroplast and nuclear dna from dark- and light-grown, control- and 3-amino-1,2,4-triazole-treated wheat seedlings, triticum vulgare, is described. contrary to a previous report, we found that chloroplast and nuclear dna had similar buoyant densities (1.702 grams per cubic centimeter) and that they could not be resolved by buoyant density centrifugation in cscl. difference in renaturation behavior of the chloroplast and nuclear dna was used as the criterion for distinguishing o ... | 1970 | 5500210 |
| ethyl methanesulfonate-induced chlorophyll mutations in triticum aestivum. | 1970 | 5512562 | |
| durum-type wheat with high bread-making quality. | a durum-type wheat (2n = 4x = 28) with high bread-making quality was produced by crossing a durum-wheat variety with a common bread-wheat variety (2n = 6x = 42), backcrossing to the bread-wheat variety for three generations, and then selecting for 28-chromosome plants. the high quality is tentatively attributed to a translocation involving one of the d-genome chromosomes. | 1968 | 5634916 |
| the action of sirmate (3,4-dichlorobenzyl methylcarbamate) on chloroplast ribosomes of triticum vulgare l. seedlings. | 1968 | 5656411 | |
| the determination of tocopherols and isoprenoid quinones in the grain and seedlings of wheat (triticum vulgare). | 1. a comparison is made of several procedures for the extraction of tocopherols and isoprenoid quinones from plant tissues. 2. gradient-elution column chromatography on acid-washed alumina efficiently separates the isoprenoid quinones and tocopherols into groups that can then be assayed spectrophotometrically or, with the tocopherols, separated into their individual components and determined by gas-liquid chromatography. 3. this improved analytical procedure was used to study the distribution of ... | 1968 | 5667256 |
| the pattern and control of isoprenoid quinone and tocopherol metabolism in the germinating grain of wheat (triticum vulgare). | 1. the syntheses of ubiquinone-9 and plastoquinone-9 were used as parameters respectively of mitochondrial and proplastid development in the germinating wheat grain. 2. the changes in the amounts of the tocopherols were also studied and the possible biological significance of these changes is discussed. during germination, the dimethyl tocopherols of the resting grain are probably not utilized for the synthesis of alpha-tocopherol. 3. it was demonstrated that ubiquinone synthesis, and hence prob ... | 1968 | 5667257 |
| ethyl alcohol and mutation in the meiosis of triticum vulgare var. giza. | 1968 | 5672719 | |
| peroxidase isoenzyme associated with the aegilops umbellulata chromosome segment transferred to chinese spring (triticum aestivum). | 1968 | 5697752 | |
| [inhibitory action of ethanol on germination of rice (oryza sativa l.) and wheat (triticum vulgare) seeds]. | 1968 | 5761008 | |
| carbon dioxide compensation points in related plant species. | both high and low c0(2) compensation concentrations were found in the plant genera-panicum, cyperus, and euphorbia. within each genus, however, high and low compensations were found in different subgenera. thus, they may not be genetically closely related. no significant differences in co(2) compensation were found among 100 genetic lines of triticum aestivum l. or among 20 lines of hordeum vulgare l. | 1969 | 5774193 |
| wheat proteins. ii. changes in the protein composition of triticum vulgare during the life cycle of the plant. | 1965 | 5827911 | |
| [aneuploidy in triticum aestivum l]. | 1964 | 5899970 | |
| [content of magnesium and other elements in plants (agropyrum repens p. beauv., secale cereale l., triticum aestivum l.) influenced by magnesite exhalation products]. | 1966 | 5912899 | |
| particle arrangements in proplastids of triticum vulgare l. seedlings. | particles having ribosome-like characteristics are described in proplastids of dark-grown wheat seedlings as the membranes of the prolamellar body become transformed, under the influence of light, into grana and fret membranes. three arrangements of particles were noted: (1) a random distribution of discrete particles; (2) particles occurring in helices or parallel rows; and (3) particles arranged in rough squares with six to eight particles per side. it is possible that the third type of partic ... | 1967 | 6039369 |
| effect of 5bs in suppressing the expression of altered dosage of 5blon meiotic chromosome pairing in triticum aestivum. | 1967 | 6050671 | |
| use of a recombination-deficient phage lambda system to construct wheat genomic libraries. | the poor cloning efficiency of wheat (triticum aestivum cv. yamhill) dna in conventional cloning vectors has previously prevented the preparation of complete genomic libraries. we show here that while wheat dna does not clone efficiently using the vector ch4a, it can be cloned efficiently using ch32. ch32 clones are red- gam+ and therefore can be propagated on recombination-deficient hosts. these results suggest that instability of wheat sequences in conventional lambda vector systems has frustr ... | 1984 | 6096216 |
| [catalytic concentration, multiple forms, and lectin affinity of microsomal enzymes from human tissues: lectins as reagents, ii (author's transl)]. | we estimated the concentrations, multiple forms, and lectin binding of five microsomal enzymes in particle free extracts from human kidney, pancreas, jejunal mucosa, and normal and cancerous liver. while arylesterase markedly reacted only with concanavalin a, arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase were intensely precipitated by lectins from ricinus communis 120, canavalia ensiformis, triticum vulgare and phaseolus vulgaris s. agglutinins from glycine max ... | 1981 | 6120206 |
| celiac sprue: correlation with murine t cell responses to wheat gliadin components. | celiac sprue is a disease in humans that is characterized by small intestinal mucosal injury and malabsorption. dietary exposure to gliadin and similar proteins in rye and barley activates the disease in susceptible individuals. celiac sprue appears to be the only disease with a marked hla-association in which the proteins that activate the disease currently are well known. however, bread wheat gliadins are a complex mixture of proteins that contain at least 40 different components. in the prese ... | 1982 | 6183341 |
| cytochemical localization of small intestinal glycoconjugates by lectin histochemistry in controls and subjects with cystic fibrosis. | human mucosal glycoconjugates were examined in normal small intestinal biopsies from five control subjects using six different fluorescein-conjugated lectins: triticum vulgare agglutinin (wga), ulex europaeus agglutinin i (uea1), ricinus communis agglutinin i (rca1), glycin max-soy bean agglutinin (sba), dolichus biflorus agglutinin (dba), and arachis hypogaea peanut agglutinin (pna). these plant agglutinins bind to specific nonreducing end-terminal carbohydrate residues. only the lectins derive ... | 1983 | 6188589 |