Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| isolation, molecular and biological properties of a lectin from rice embryo: relationship with wheat germ agglutinin properties. | rice lectin (oryza sativa, var. balilla 28) was purified from defatted embryos by aqueous acid extraction at ph 1.3 followed by ammonium sulfate precipitation between 2 and 4 m, affinity chromatography on agarose-p-aminophenyl-beta-d-n-acetylglucosamine, and gel filtration on aca 54. the homogeneity of the lectin was checked by polyacrylamide gel electrophoresis, gel filtration, and immunodiffusion. the amino acid analysis revealed a high half-cystine content (9%) and a low aromatic and hydropho ... | 1987 | 3688888 |
| effect of zinc deficiency on kinetics of ribulose-1,5-biphosphate carboxylase of oryza sativa l. and pennisetum americanum (l.) leeke. | 1985 | 4030003 | |
| interaction of rice (oryza sativa) lectin with n-acetylglucosaminides. fluorescence studies. | the interaction of lectin isolated from rice (oryza sativa) embryos with n-acetylglucosaminides was studied by equilibrium dialysis and fluorescence. equilibrium dialysis with 4-methylumbelliferyl-(glcnac)2 showed that rice lectin (mr 38000) contains four equivalent saccharide-binding sites. addition of the n-acetylglucosaminides glcnac, (glcnac)2 and (glcnac)3 enhanced the intrinsic fluorescence of rice lectin and this was accompanied by a 10nm blue-shift of its maximum fluorescence with (glcna ... | 1985 | 4052018 |
| [phenomenon of radioprotection by previous imbibition at low temperature observed in rice seeds (oryza sativa l.) subjected to gamma rays of co60]. | 1974 | 4210673 | |
| effect of anaerobiosis on respiratory rate, cytochrome oxidase activity and mitochondrial structures in coleoptiles of rice (oryza sativa l.). | 1973 | 4352691 | |
| correlation between the age related metabolites and photoinduction in oryza sativa. | 1974 | 4442472 | |
| complementary genic complex for anthocyanin pigmentation in the apiculus of rice (oryza sativa l.). | 1973 | 4781370 | |
| leaf surface fungi of oryza sativa linn. | 1971 | 5105623 | |
| effect of certain chemical fertilizers on the rhizosphrere mycoflora of oryza sativa linn. ii. potassium fertilizer (potassium sulphate). | 1971 | 5129428 | |
| [anaerobic phenomena in plants. ii. experimental studies on the relationships between anaerobiosis phenomena and catalase in caryopses of oryza sativa]. | 2015 | 5146120 | |
| investigations into rhizosphere microflora. i. succession of microflora on root regions of oryza sativa l. | 2000 | 5159954 | |
| phytotoxicity tests with the molluscicide n-tritylmorpholine. | n-tritylmorpholine (frescon, wl 8008) has been applied as an emulsifiable concentrate (fx 28) to cotton and rice in glasshouse experiments without any adverse effects. the compound was applied in the irrigation water at 5 and 10 times the rates used for snail control. the experiment on cotton was terminated after 60 days, when the plants became pot-bound. the treatments did not affect the leaf area or the fresh weight of plants. the rice (oryza sativa ssp. japonica) was harvested and no differen ... | 1967 | 5300052 |
| phytochrome action in oryza sativa l. 3. the separation of photoperceptive site and growing zone in coleoptiles, and auxin transport as effector system. | 1969 | 5359044 | |
| mutagen-induced variability in protein characters in oryza sativa. | 1970 | 5433772 | |
| effect of certain chemical fertilizers on the rhizosphere myco-flora of oryza sativa. i. nitrogenous fertilizers: ammonium nitrate and urea. | 1971 | 5575838 | |
| metabolism of 3,4-dichloropropionanilide in plants: the metabolic fate of the 3,4-dichloroaniline moiety. | studies to elucidate the fate of 3,4-dichloropropionanilide (propanil) in rice (oryza sativa l. var. nato) plants have shown that the propanil molecule is cleaved and the propionic acid moiety metabolized. to ascertain the fate of the 3,4-dichloroaniline moiety of propanil, rice plants were exposed to propanil in liquid culture. the roots and shoots of treated rice plants were extracted and quantitatively assayed for four aniline-containing metabolites. one of the four metabolites proved to be 3 ... | 1968 | 5635999 |
| metabolism of 3',4'-dichloropropionanilide: 3,4-dichloroaniline-lignin complex in rice plants. | rice plants (oryza sativa l. var. bluebonnet 50) metabolize the herbicide 3',4'-dichloropropionanilide to 3,4-dichloroaniline, which in turn conjugates with carbohydrates. soluble aniline-carbohydrate complexes account for only a small fraction of the hydrolyzed 3',4'-dichloropropionanilide. the major portion of the 3,4-dichloroaniline moiety is found complexed with polymeric cell constituents, mainly lignin. the aniline is lignin-bound as 3,4-dichloroaniline and not as 3',4'-dichloropropionanil ... | 1968 | 5661296 |
| mechanism of selective action of 3',4'-dichloropropionanilide. | studies have been carried out to determine the basis for the unique postemergence selective action of the rice herbicide, 3',4'-dichloropropionanilide (dpa), which controls a wide variety of weed species including barnyardgrasses. absorption studies have shown that both rice (oryza sativa, l.) and barnyardgrass (echinochloa crusgalli, l.) foliage absorb dpa readily and equally and, consequently, morphological differences cannot account for its unique selective action. through the use of uniforml ... | 1968 | 5700023 |
| [inhibitory action of ethanol on germination of rice (oryza sativa l.) and wheat (triticum vulgare) seeds]. | 1968 | 5761008 | |
| effect of water washing, lime treatment and lime and calcium carbonate supplementation on the nutritive value of paddy (oryza sativa) straw. | 1969 | 5818438 | |
| cytochrome oxidase subunit ii gene of rice has an insertion sequence within the intron. | we have isolated and sequenced the cytochrome oxidase subunit ii gene from rice (oryza sativa l. var labelle). the overall structural organization of this gene is very similar to that of the maize gene. this gene contains an intron in a position identical to the intron in the maize gene. however, the intron in the rice gene is longer than that of the maize gene largely due to a 461 bp insertion sequence, which has inverted repeats at its termini and is flanked by direct repeats, characteristic o ... | 1984 | 6093039 |
| influence of streptomycin on alpha-amylase formation isolated endosperms & protein pattern in intact seedlings of rice oryza sativa l. | 1980 | 6159319 | |
| amino acid sequence of cytochrome c from rice. | the amino acid sequence of cytochrome c purified from rice, oryza sativa l., was determined. the complete amino acid sequence of rice cytochrome c is as follows: ac-ala-8-ser-phe-ser-glu-ala-pro-pro-gly1-asn-pro-lys-ala-gly-glu-lys-ile-phe10-lys-thr-lys-cys-ala-glx-cys-his-thr-val20-asp-lys-gly-ala-gly-his-lys-glx-gly-pro30-asx-leu-asx-gly-leu-phe-gly-arg-glx-ser40-gly-thr-thr-pro-gly-tyr-ser-tyr-ser-thr50-ala-asp-lys-asn-met-ala-val-ile-trp-glx60-glx-asx-thr-leu-tyr-asp-tyr-leu-leu-asn70-pro-tm ... | 1980 | 6244261 |
| [evaluation of nutritional potential of fish in diets based on beans (phaseolus vulgaris) and a cereal/corn (zea mays) and/or rice (oryza sativa)]. | p6e complementation between corn and fish, and rice and fish was evaluated for the purpose of establishing the required levels of each constituent in the mixture to obtain the optimum value in the biological evaluations for each diet. the optimum fish levels were around 10 and 6% for the corn and rice diets, respectively. complementary levels of fish in the bean: corn and bean:rice diets were evaluated in the same manner. the best values in the biological evaluation were obtained with only 2% of ... | 1983 | 6675550 |
| active polypeptide fragments common to prokaryotic, eukaryotic, and mitochondrial dna polymerases. | with a procedure that allows the renaturation of the dna polymerase catalytic activity in situ after sds-polyacrylamide gel electrophoresis, we have compared the active polypeptides present in extracts from organisms covering a wide evolutionary range from prokaryotes to eukaryotes, namely: escherichia coli, oryza sativa, daucus carota , neurospora crassa, dictyostelium discoideum, saccharomyces cerevisiae, ceratitis capitata, leucophaea maderae , xenopus laevis, rat tissues and human lymphoblas ... | 1982 | 6765191 |
| [microbiological determination of choline in rice (oryza sativa)]. | 1981 | 6982631 | |
| lipid molecular species composition of thylakoid membranes. | lipid molecular species compositions of chloroplast thylakoid membranes of mesophyll cells from spinacia oleracea, glycine max, oryza sativa and zea mays and of bundle sheath cells from zea mays have been quantitatively determined. no significant difference in the lipid molecular species composition was found among the five membrane sources. the predominant molecular species of monogalactosyldiacylglycerol was the 1-linolenoyl parallel to 2-linolenoyl species. the 1-linolenoyl parallel to 2-lino ... | 1980 | 7188732 |
| effect of fluoride on in vivo nitrate reduction in the rive leaves (oryza sativa l.). | fluoride had no effect on in vitro nitrate reductase activity in rice leaves, but in vivo activity was strongly inhibited. it is suggested that fluoride brings about this inhibition by adversely affecting the physiological nadh generating system required for in vivo nitrate reduction. | 1980 | 7191373 |
| effects of toxic concentrations of nickel on growth & metabolism of rice (oryza sativa l.) seedlings. | 1980 | 7399618 | |
| repetitive dna in three gramineae species with low dna content. | the genomes of three gramineae species, namely finger millet (eleusine coracana), pearl millet (pennisetum americanum) and rice (oryza sativa) are characterized by studying their dna denaturation-reassociation properties. the reassociation kinetics measurement of the sonicated dna (500--700 nucleotide pairs) indicate the presence of a heterogeneous, repetitive dna fraction accounting for 49--54% of the total dna in all three species. from the cot 1/2 value of the slow reassociating dna, the geno ... | 1980 | 7409756 |
| nramp defines a family of membrane proteins. | nramp (natural resistance-associated macrophage protein) is a newly identified family of integral membrane proteins whose biochemical function is unknown. we report on the identification of nramp homologs from the fly drosophila melanogaster, the plant oryza sativa, and the yeast saccharomyces cerevisiae. optimal alignment of protein sequences required insertion of very few gaps and revealed remarkable sequence identity of 28% (yeast), 40% (plant), and 55% (fly) with the mammalian proteins (46%, ... | 1995 | 7479731 |
| identification and gene expression of anaerobically induced enolase in echinochloa phyllopogon and echinochloa crus-pavonis. | enolase (2-phospho-d-glycerate hydrolase, ec 4.2.1.11) has been identified as an anaerobic stress protein in echinochloa oryzoides based on the homology of its internal amino acid sequence with those of enolases from other organisms, by immunological reactivity, and induction of catalytic activity during anaerobic stress. enolase activity was induced 5-fold in anoxically treated seedlings of three flood-tolerant species (e. oryzoides, echinochloa phyllopogon, and rice [oryza sativa l.]) but not ... | 1995 | 7480340 |
| a comparative study of cadmium, lead, zinc, ph, and bulk density from the enyigba lead and zinc mine in two different seasons. | soil ph and bulk density, as well as the concentrations of cadmium (cd), lead (pb), and zinc (zn) in soils and these metal levels in the leaves of cassava (manihot esculenta) and rice (oryza sativa), both collected, respectively, in the dry season (december) of 1991 and the rainy season (september) of 1992 from the enyigba lead and zinc mine, nigeria, were investigated. interspecific variations in trace element levels in the plant leaves were detected and may reflect the predilection of these pl ... | 1995 | 7498063 |
| a novel lipoxygenase from rice. primary structure and specific expression upon incompatible infection with rice blast fungus. | a novel lipoxygenase cdna (3,007 base pairs) was isolated from rice leaves (oryza sativa cv. aichiasahi) which had been infected with an incompatible race of the rice blast fungus, magnaporthe grisea. a single copy of the gene is present in the rice genome and encodes a protein of 923 residues with a molecular weight of 102,714. this gene product shares the least amino acid sequence homology among plant lipoxygenases identified to date. a novel feature of this gene product is a putative transit ... | 1994 | 7508918 |
| palindromic repeated sequences (prss) in the mitochondrial genome of rice: evidence for their insertion after divergence of the genus oryza from the other gramineae. | we have identified a family of small repeated sequences (from 60 to 66 bp in length) in the mitochondrial genome of rice (oryza sativa cv. nipponbare). there are at least ten copies of these sequences and they are distributed throughout the mitochondrial genome. each is potentially capable of forming a stem-and-loop structure and we have designated them prss (palindromic repeated sequences). their features are reminiscent of the small dispersed repeats in the mitochondrial dna (mtdna) of some lo ... | 1994 | 7509206 |
| identification, cdna cloning, and gene expression of soluble starch synthase in rice (oryza sativa l.) immature seeds. | three forms of soluble starch synthase were resolved by anion-exchange chromatography of soluble extracts from immature rice (oryza sativa l.) seeds, and each of these forms was further purified by affinity chromatograph. the 55-, 57-, and 57-kd proteins in the three preparations were identified as candidates for soluble starch synthase by western blot analysis using an antiserum against rice granule-bound starch synthase. it is interesting that the amino-terminal amino acid sequence was identic ... | 1993 | 7518089 |
| an epitope of rice threonine- and hydroxyproline-rich glycoprotein is common to cell wall and hydrophobic plasma-membrane glycoproteins. | a monoclonal antibody, lm1, has been derived that has a high affinity for an epitope of hydroxyproline-rich glycoproteins (hrgps). in suspension-cultured rice (oryza sativa l.) cells the epitope is carried by three major proteins with different biochemical properties. the most abundant is the 95-kda extracellular rice extensin, a threonine- and hydroxyproline-rich glycoprotein (thrgp) occurring in the cell wall and secreted into the medium. this thrgp can be selectively oxidatively cross-linked ... | 1995 | 7544182 |
| purification and characterization of phospholipase d (pld) from rice (oryza sativa l.) and cloning of cdna for pld from rice and maize (zea mays l.). | phospholipase d (pld) was purified to high homogeneity from rice bran (oryza sativa l.). two peaks of pld activity were resolved by mono q anion-exchange chromatography. the molecular mass of pld in both peaks was 82 kda on sds-page and 78 kda in gel filtration. antibodies raised against the protein in one of the peaks precipitated the enzyme activities in both peaks. enzymatic characteristics of pld in the two peaks were identical except for a difference of 0.1 in the isoelectric points. sequen ... | 0 | 7551587 |
| polymorphism and genetic relatedness among wild and cultivated rice species determined by ap-pcr analysis. | we have applied the arbitrarily primed polymerase chain reaction (ap-pcr) technique to the analysis of the relationships among six japonica and indica cultivars, and four wild species of rice. chosen were four primers of arbitrary sequence that gave multiple amplification products when rice dna was used as template. among a total of 50 bands scored, 44 were polymorphic, which was sufficient to distinguish the species used in this study. it is apparent from the comparisons of genetic distances th ... | 1995 | 7558882 |
| neighboring base composition and transversion/transition bias in a comparison of rice and maize chloroplast noncoding regions. | the correspondence between the transversion/transition ratio and the neighboring base composition in chloroplast dna is examined. for 18 noncoding regions of the chloroplast genome, alignments between rice (oryza sativa) and maize (zea mays) were generated by two different methods. difficulties of aligning noncoding dna are discussed, and the alignments are analyzed in a manner that reduces alignment artifacts. sequence divergence is < 10%, so multiple substitutions at a site are assumed to be r ... | 1995 | 7568204 |
| rice scutellum induces agrobacterium tumefaciens vir genes and t-strand generation. | for successful transformation of a plant by agrobacterium tumefaciens it is essential that the explant used in cocultivation has the ability to induce agrobacterium tumour-inducing (ti) plasmid virulence (vir) genes. here we report a significant variation in different tissues of indica rice (oryza sativa l. cv. co43) in their ability to induce agrobacterium tumefaciens vir genes and t-strand generation, using explants preincubated in liquid murashige and skoog (ms) medium. an analysis of rice le ... | 1995 | 7579158 |
| an amino-terminal deletion of rice phytochrome a results in a dominant negative suppression of tobacco phytochrome a activity in transgenic tobacco seedlings. | overexpression of phytochrome a results in an increased inhibition of hypocotyl elongation under red and far-red light. we used this approach to assay for the function of n-terminal mutations of rice (oryza sativa l.) phytochrome a. transgenic tobacco seedlings that express the wild-type rice phytochrome a (rw), a rice phytochrome a lacking the first 80 amino acids (ntd) or a rice phytochrome a with a conversion of the first 10 serines into alanine residues (s/a) were compared with untransformed ... | 1995 | 7580859 |
| 2,3-bisphosphoglycerate-independent phosphoglycerate mutase is conserved among different phylogenic kingdoms. | we have previously demonstrated that maize (zea mays) 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (pgam-i) is not related to 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase. with the aid of specific anti-maize pgam-i antibodies, we demonstrate here the presence of a closely related pgam-i in other plants. we also describe the isolation and sequencing of a cdna-encoding almond (prunus amygdalus) pgam-i that further demonstrates this relationship among plant pgam-i. a sea ... | 1995 | 7584858 |
| the regulatory regions of the rice tungro bacilliform virus promoter and interacting nuclear factors in rice (oryza sativa l.). | the rice tungro bacilliform virus (rtbv) promoter confers phloem-specific gene expression in transgenic rice plants. a series of promoter deletion mutants were fused with the escherichia coli beta-glucuronidase a (uida) reporter gene and introduced into transgenic rice plants. the rtbv promoter confers substantially stronger expression in shoots than in roots. a fragment of the promoter comprising nucleotides -164 to +45 relative to the transcriptional start site contains sufficient information ... | 1995 | 7599653 |
| fractionation and characterization of two forms of peroxidase from oryza sativa. | peroxidase (e.c. 1.11.1.7., hydrogen donor oxidoreductase) is widely distributed and has been isolated from many higher plants (1). the wide distribution of the enzyme suggests that it could be of great biological importance. however the role that it plays in metabolism is not clear due to the large number of reactions it catalyzes and the considerable number of isozymic species (2). in tomato plants, evans and aldridge (3) separated out six isoperoxidases and in a later paper evans reported 12 ... | 1995 | 7603969 |
| vacuolar h(+)-translocating pyrophosphatase is induced by anoxia or chilling in seedlings of rice. | the present study was undertaken to determine whether vacuolar h(+)-pyrophosphatase (v-ppase) might replace vacuolar h(+)-atpase under energy stress due to anoxia or chilling in anoxia-tolerant species such as rice (oryza sativa l.) and corn (zea mays l.). the relative transcript level of v-ppase in rice seedlings, like that of alcohol dehydrogenase 1, increased greatly under anoxia and declined again when the seedlings were returned to air. however, the distribution of transcripts in root, shoo ... | 1995 | 7610161 |
| structure, organization, and chromosomal location of the gene encoding a form of rice soluble starch synthase. | a rice (oryza sativa l.) genomic clone encoding the gene for a form of soluble starch synthase (sss1) and its 5'- and 3'-flanking regions has been isolated and sequenced. the sss1 gene contained 15 exons interrupted by 14 introns. the exon/intron organization of the sss1 gene was divergent from that of the rice waxy gene coding for granule-bound starch synthase, thus suggesting that the sss1 and granule-bound starch synthase genes have evolved from an ancestral gene in a different way or that th ... | 1995 | 7610165 |
| a rice membrane calcium-dependent protein kinase is induced by gibberellin. | a rice (oryza sativa) seed plasma-membrane calcium-dependent serine/threonine protein kinase (cdpk) has been partially purified. comparing results in seeds that were treated with and without the plant hormone gibberellin (ga) for 10 min showed that rice cdpk was highly induced by ga. after separating solubilized membrane proteins by sodium dodecyl sulfate-gel electrophoresis, followed by renaturation, a radiolabeled phosphoprotein band of approximately 58 kd was detected, and it was apparently p ... | 1995 | 7610167 |
| a novel stress-inducible metallothionein-like gene from rice. | a novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from oryza sativa genomic library. this sequence, hereby designated as rgmt (rice genomic metallothionein-like gene), consists of two exons and one intron. from the coding sequence, it is predicted that rgmt encodes one protein of 74 amino acids. differential expression of rgmt in rice plants was observed as mature transcripts were more abundant in roots than in leaves and sheaths. ... | 1995 | 7632910 |
| presence of a chloroplast dna sequence in an autonomous circular dna molecule in cultured rice cells (oryza sativa l). | sequence analysis of twelve dna fragments, which had previously been found to be extensively amplified in suspension-cultured rice cells, revealed that two of them, isolated on plasmids designated pe10 and pe11, have sequences identical to distinct regions of chloroplast dna (ct-dna). both sequences are part of an extrachromosomal circular dna molecule (ecd). the molecular structure of the ecd was investigated by a combination of restriction analysis, standard and pulsed-field gel electrophoresi ... | 1995 | 7640893 |
| complete sequence of the maize chloroplast genome: gene content, hotspots of divergence and fine tuning of genetic information by transcript editing. | the nucleotide sequence of the chloroplast (cp) dna from maize (zea mays) has been completed. the circular double-stranded dna, which consists of 140,387 base-pairs, contains a pair of inverted repeat regions (ira and irb) with 22,748 base-pairs each, which are separated by a small and a large single copy region (ssc and lsc) of 12,536 and 82,355 base-pairs, respectively. the gene content and the relative positions of a total of 104 genes (70 peptide-encoding genes, 30 trna genes and four rrna g ... | 1995 | 7666415 |
| a view of plant dehydrins using antibodies specific to the carboxy terminal peptide. | dehydrins are characterized by the consensus kikeklpg amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. a synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. this range covered two families of monocots, viz. gramineae (hordeum vulgare l., triticum aestivum l., zea mays l., oryza sativa l.) and liliaceae (allium sativa l.), and five famili ... | 1993 | 7693020 |
| identification and characterization of a family of genes for the plasma membrane h(+)-atpase of oryza sativa l. | a cdna clone (cosa2) encoding a plasma membrane h(+)-atpase was isolated from rice. southern blot analysis indicated that the genes that corresponds to cosa2 was different from that to cosa1. northern blots revealed osa2 mrna in roots, calli and shoots. osa1 transcripts were detected only by rt-pcr in these tissues. | 1994 | 7697296 |
| nucleotide sequence of rice (oryza sativa l.) cdna homologous to cdc2 gene. | we isolated and determined a nucleotide sequence of a rice dna clone (ss224) denoted to rcdc2. this clone encodes an open reading frame of 302 amino acids and typical three conserved domains that exist in all cdc2 homologues. the evolutionary tree showed that rcdc2 was far from cdc2 and its homologous genes identified in various plants. | 1994 | 7703506 |
| use of rapd for the study of diversity within plant germplasm collections. | as part of the development of a molecular toolkit for the study of diversity within large plant germplasm collections, rapd technology has been applied to accessions of rice (oryza sativa) obtained from the major world collection held at irri (the international rice research institute) which supplies germplasm to breeders. methods for the speedy extraction of dna representative of a rice accession, its amplification by pcr to reveal reproducible products, and the analysis of the banding data usi ... | 1995 | 7706109 |
| [pollen of gramineae and its botanical, taxonomical, and ecological aspects]. | this is a review of tropical pollens (gramineae) which cause allergy, addressed to medical workers and with emphasis in the costa rican. the graminean family poaceae is widely distributed and has great genetic plasticity. an evolutionary tendency to floral reduction has led to anemophily, with pollens ranging from large (e.g. corn, zea mays) to very small (e.g. tribes poae and avenae). this family produces a large number of pollens per panicle. the early morning production of free pollen (before ... | 1994 | 7708965 |
| molecular cloning of the gene (sodcc1) that encodes a cytosolic copper/zinc-superoxide dismutase from rice (oryza sativa l.). | 1995 | 7724677 | |
| proline accumulates in plants exposed to uv radiation and protects them against uv induced peroxidation. | proline accumulated in the shoots of seedlings of rice (oryza sativa), mustard (brassica juncea) and mung bean (vigna radiata) exposed to uv radiations. the level of proline in the seedlings increased significantly with increase in uv exposure time. the production of malondialdehyde (an indice of lipid peroxidation) was also higher in the shoots of seedlings exposed to uv radiation as compared to controls, suggesting that uv radiations promote lipid peroxidation. the extent of uv radiation promo ... | 1995 | 7726821 |
| the atx1 gene of saccharomyces cerevisiae encodes a small metal homeostasis factor that protects cells against reactive oxygen toxicity. | in aerobic organisms, protection against oxidative damage involves the combined action of highly specialized antioxidant enzymes, such as superoxide dismutase (sod) and catalase. here we describe the isolation and characterization of another gene in the yeast saccharomyces cerevisiae that plays a critical role in detoxification of reactive oxygen species. this gene, named atx1, was originally isolated by its ability to suppress oxygen toxicity in yeast lacking sod. atx1 encodes a 8.2-kda polypep ... | 1995 | 7731983 |
| gibberellin promotes histone h1 kinase activity and the expression of cdc2 and cyclin genes during the induction of rapid growth in deepwater rice internodes. | partial submergence or treatment with either ethylene or gibberellin (ga) promotes rapid internodal growth in deepwater rice (oryza sativa l.). earlier work has shown that ga is the immediate hormonal signal for this growth response, which involves induction of the cell cycle at the g2/m phase transition and subsequent enhancement in the rate of dna synthesis. in all eukaryotes, onset of mitosis is regulated by the p34cdc2/cdc28 protein kinase, whose activity is assayed by in vitro phosphorylati ... | 1995 | 7742859 |
| the genomic organization of the gene encoding a nitrate-inducible ferredoxin-nadp+ oxidoreductase from rice roots. | a genomic clone of the gene encoding a nitrate-inducible ferredoxin-nadp+ oxidoreductase (fnr) from rice (oryza sativa l.) roots has been isolated and its nucleotide sequence determined. the clone contains 3897 nucleotides of the gene which consists of six exons interrupted by five introns. the transcription start site was determined by primer extension analysis which locates 64 bp upstream of the atg translation initiation codon. the 5'-flanking region contains canonical tata- and caat-boxes, a ... | 1995 | 7748889 |
| metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes. | fluorescence in situ hybridization (fish) is a powerful tool for physical mapping in human and other mammalian species. however, application of the fish technique has been limited in plant species, especially for mapping single- or low-copy dna sequences, due to inconsistent signal production in plant chromosome preparations. here we demonstrate that bacterial artificial chromosome (bac) clones can be mapped readily on rice (oryza sativa l.) chromosomes by fish. repetitive dna sequences in bac c ... | 1995 | 7753830 |
| six new candidate members of the alpha/beta twisted open-sheet family detected by sequence similarity to flavodoxin. | strong sequence similarity has been reported among wrba (the trp repressor-binding protein of escherichia coli); ycp4, a protein of unknown function from the budding yeast saccharomyces cerevisiae; p25, the pap1-dependent protein of the fission yeast schizosaccharomyces pombe; and the translation product of a partial cdna sequence from rice seedling root (oryza sativa, locus ricr02421a; here referred to as ricr). further homology search with the profile method indicates that all the above sequen ... | 1994 | 7756978 |
| state of the foreign gene and of the genome in transgenic rice (oryza sativa l.). | pcr with random primers (rapd analysis) performed on the dna of embryogenic and non-embryogenic suspension cultured rice and of transformed rice plants allows the evaluation of the extent of dna changes in the different biological materials. this is thus suggested as a convenient approach, in combination with restriction analysis and southerns blotting, to evaluate the integrity of the foreign gene, the stability of the insertion site and the stability of the whole genome. | 1993 | 7763739 |
| transgenic indica rice (oryza sativa l.) plants obtained by direct gene transfer to protoplasts. | we have established a reproducible procedure for transformation of protoplasts and regeneration of transgenic plants for an improved indica rice cultivar ir43. mature embryo-derived calli were placed in liquid culture medium containing maltose to establish meristematically active, embryogenic cell suspension lines. in order to obtain transgenic plants, a chimeric hygromycin phosphotransferase hph gene under the control of the cauliflower mosaic virus camv 35s promoter was introduced into protopl ... | 1994 | 7764447 |
| heat-inducible rice hsp82 and hsp70 are not always co-regulated. | we have characterized several heat-shock-induced genes in rice (oryza sativa l.) and compared their expression under a variety of conditions. three of these genes, which are analogs of the hsp82/90 family, lie within a cloned 18-kilobase (kb) region of the genome. the middle member of this cluster, designated hsp82b, has been fully sequenced. the gene uses a promoter containing six putative heat-shock elements as well as several unusual sequence motifs including a stretch of 11 thymidines altern ... | 1994 | 7764623 |
| thioredoxin h is one of the major proteins in rice phloem sap. | sieve tubes play important roles in the transfer of nutrients as well as signals. hundreds of proteins were found in pure phloem sap collected from rice (oryza sativa l. cv. kantou) plants through the cut ends of insect stylets. these proteins may be involved in nutrient transfer and signal transduction. to characterize the nature of these proteins, the partial amino-acid sequence of a 13-kda protein, named rpp13-1, that was abundant in the pure phleom sap was determined. a cdna clone of 687 bp, ... | 1995 | 7766047 |
| expression of functional proteins of cdna encoding rice nucleoside diphosphate kinase (ndk) in escherichia coli and organ-related alteration of ndk activities during rice seed germination (oryza sativa l.). | the gst (glutathione s-transferase)-ndk (nucleoside diphosphate kinase) fusion protein was expressed in escherichia coli. the gst-ndk protein was capable of transferring gamma-phosphate from atp to nucleoside diphosphates such as gdp, cdp, tdp and udp. western blot analysis using anti-ndk antibody indicated that ndk in endosperm gradually decreased during 36 h of imbibition. on the contrary, ndk in embryo increased during the same period. ndk activities in both tissues were in accord with these ... | 1995 | 7766875 |
| biochemical properties of rice adenylate kinase and subcellular location in plant cells. | previously, we characterized nucleotide sequences of two cdnas encoding adenylate kinase from rice plants (oryza sativa l.). each cdna (adk-a or adk-b) was cloned into the expression vector pet 11d-gst to produce gst-ak fusion proteins in escherichia coli. recombinant proteins were cleaved by thrombin, and gst-free adenylate kinase proteins were obtained. enzyme activity profiles of different ph and inhibition effects to the enzyme by ap5a (adenosine-5'-pentaphospho-5'-adenosine) indicates that ... | 1995 | 7766884 |
| molecular cloning and characterization of rga1 encoding a g protein alpha subunit from rice (oryza sativa l. ir-36). | a cdna clone, rga1, was isolated by using a gpa1 cdna clone of arabidopsis thaliana g protein alpha subunit as a probe from a rice (oryza sativa l. ir-36) seedling cdna library from roots and leaves. sequence analysis of genomic clone reveals that the rga1 gene has 14 exons and 13 introns, and encodes a polypeptide of 380 amino acid residues with a calculated molecular weight of 44.5 kda. the encoded protein exhibits a considerable degree of amino acid sequence similarity to all the other known ... | 1995 | 7766894 |
| molecular cloning and characterization of a cdna for the alpha subunit of a g protein from rice. | we report the isolation of a cdna for the alpha subunit of a g protein from rice (oryza sativa l. cv. nipponbare). the cdna contained an open reading frame that encoded a protein of 380 amino acid residues with a mol wt of 44,204. we designated this polypeptide rga1 (rice g protein alpha subunit 1). the amino acid sequence of rga1 was 77% and 86% identical to the sequences of alpha subunits from arabidopsis thaliana and tomato (products of gpa1 and tga1), respectively, and 42% to 69% identical t ... | 1995 | 7767602 |
| rice cationic peroxidase accumulates in xylem vessels during incompatible interactions with xanthomonas oryzae pv oryzae. | a cationic peroxidase, po-c1 (molecular mass 42 kd, isoelectric point 8.6), which is induced in incompatible interactions between the vascular pathogen xanthomonas oryzae pv oryzae and rice (oryza sativa l.), was purified. amino acid sequences from chemically cleaved fragments of po-c1 exhibited a high percentage of identity with deduced sequences of peroxidases from rice, barley, and wheat. polyclonal antibodies were raised to an 11-amino acid oligopeptide (poc1a) that was derived from a domain ... | 1995 | 7770527 |
| bamhi and hindiii repetitive dna families in the rice genome. | in this paper we describe a novel method of cloning representative members of different repetitive dna families based on the screening of a rice (oryza sativa) genomic library using dna from different c0t fractions as probes. two genomic clones, which represent two different repetitive dna families in rice, were isolated by this method and have been characterized. their nucleotide sequences, copy numbers, distributions in major rice genome types, methylation patterns, and chromosomal localizatio ... | 1995 | 7774793 |
| the human archain gene, arcn1, has highly conserved homologs in rice and drosophila. | a novel human gene, arcn1, has been identified in chromosome band 11q23.3. it maps approximately 50 kb telomeric to mll, a gene that is disrupted in a number of leukemia-associated translocation chromosomes. cdna clones representing arcn1 hybridize to 4-kb mrna species present in all tissues tested. sequencing of cdnas suggests that at least two forms of mrna with alternative 5' ends are present within the cell. the mrna with the longest open reading frame gives rise to a protein of 57 kda. alth ... | 1995 | 7782067 |
| improvement in hc1-extractability of minerals in home made weaning foods. | three weaning foods were formulated from locally available cereals and pulses such as rice (oryza sativa), kangini (setaria italica), sanwak (echinochloa frumentacea), green gram (vigna radiata) and jaggery. cereals and pulses were mixed in the proportion of 7:3. nutrient composition of developed weaning foods was within range prescribed by indian standard institute and was found to be acceptable. roasting was the processing technique employed in developing weaning foods which resulted in signif ... | 1995 | 7792266 |
| structure and differential response to abscisic acid of two promoters for the cytosolic copper/zinc-superoxide dismutase genes, sodcc1 and sodcc2, in rice protoplasts. | we determined the 5'-flanking sequences of two nuclear genes (sodcc1 and sodcc2) encoding cytosolic copper/zinc-superoxide dismutase in rice (oryza sativa l.). utilizing transient beta-glucuronidase (gus) reporter assays, functional promoter-gus analysis was performed in rice protoplasts exposed to the phytohormone abscisic acid (aba) or the antioxidant sulfhydryl reagent, dithiothreitol (dtt). transcriptional activities from both sodcc-gus fusions were stimulated by dtt, which induces the promo ... | 1995 | 7821431 |
| synthesis of an infectious full-length cdna clone of rice yellow mottle virus and mutagenesis of the coat protein. | a full-length cdna clone of rice yellow mottle sobemovirus (rymv) was synthesized and placed adjacent to a bacteriophage t7 rna polymerase promoter sequence. capped-rna transcripts produced in vitro were infectious when mechanically inoculated onto rice plants (oryza sativa l). individual full-length clones varied in their degree of infectivity but all were less infectious than native viral rna. a representative clone, designated rymv-fl5, caused a disease phenotype identical to that produced by ... | 1995 | 7831766 |
| protein quality of weaning foods based on locally available cereal and pulse combination. | locally available cereals and pulses such as rice (oryza sativa), kangini (setaria italica), sanwak (echinochloa frumentacea), green gram (vigna radiata) and jaggery were used to formulate three weaning foods. cereal, pulse and jaggery were mixed in the ratio of 70:30:25. roasting was the main processing technique used in the formulation of these weaning foods. the developed weaning foods had 5.06 to 5.68 g moisture, 10.28 to 13.71 g protein, 2.91 to 3.77 g ash, 1.08 to 1.87 g fat, 14.42 to 14.9 ... | 1994 | 7855096 |
| characterization of an inducible p450 hydroxylase involved in the rice diterpene phytoalexin biosynthetic pathway. | ent-isopimara-8(14),15-dien-3 beta-ol (1) was isolated from uv-irradiated rice (oryza sativa l.) leaves. since 1 was converted to the rice diterpene phytoalexins and oryzalexins d (ent-isopimara-8(14),15-diene-3 beta,7 alpha-diol) and e (ent-isopimara-8(14),15-diene-3 beta,9 alpha-diol) in uv-irradiated rice leaf microsome fraction in the presence of oxygen and nadph, it was concluded that 1 was the biosynthetic precursor of these oryzalexins. this enzyme reaction was inhibited by cytochrome p45 ... | 1995 | 7864625 |
| inheritance of gusa and neo genes in transgenic rice. | inheritance of foreign genes neo and gusa in rice (oryza sativa l. cv. ir54 and radon) has been investigated in three different primary (t0) transformants and their progeny plants. t0 plants were obtained by co-transforming protoplasts from two different rice suspension cultures with the neomycin phosphotransferase ii gene [neo or aph (3') ii] and the beta-glucuronidase gene (uida or gusa) residing on separate chimeric plasmid constructs. the suspension cultures were derived from callus of immat ... | 1995 | 7865799 |
| molecular and physiological responses to abscisic acid and salts in roots of salt-sensitive and salt-tolerant indica rice varieties. | the indica rice (oryza sativa l.) varieties pokkali and nona bokra are well-known salt tolerance donors in classical breeding. in an attempt to understand the molecular basis of their tolerance, physiological and gene expression studies were initiated. the effect of abscisic acid (aba) on total proteins in roots from 12-d-old seedlings of pokkali, nona bokra, and the salt-sensitive cultivar taichung n1 were analyzed on two-dimensional gels. the abundance of aba-induced proteins was highest in th ... | 1995 | 7870812 |
| characterization of a rice gene family encoding root-specific proteins. | two cdna clones (rcc2 and rcc3) corresponding to mrnas highly expressed only in root tissues of rice (oryza sativa l.) seedlings were characterized. respectively, they encode polypeptides of 146 (14.5 kda) and 133 amino acids (13.4 kda) that share high (> 70%) sequence similarity with a polypeptide encoded by a cdna (zrp3) encoding an mrna preferentially expressed in young maize roots. genomic dna blot analysis revealed that they are members of a small gene family and rcg2, the gene correspondin ... | 1995 | 7888615 |
| novel drosophila melanogaster genes encoding rrm-type rna-binding proteins identified by a degenerate pcr strategy. | we are interested in identifying drosophila melanogaster rna-binding proteins involved in important developmental decisions made at the level of mrna processing, stability, localization or translational control. a large subset of the proteins known to interact with specific rna sequences shares an evolutionarily conserved 80-90-amino-acid (aa) domain referred to as an rna-recognition motif (rrm), including two ribonucleoprotein identifier sequences known as rnp-1 and rnp-2. hence, we have herein ... | 1995 | 7890163 |
| saturated molecular map of the rice genome based on an interspecific backcross population. | a molecular map has been constructed for the rice genome comprised of 726 markers (mainly restriction fragment length polymorphisms; rflps). the mapping population was derived from a backcross between cultivated rice, oryza sativa, and its wild african relative, oryza longistaminata. the very high level of polymorphism between these species, combined with the use of polymerase chain reaction-amplified cdna libraries, contributed to mapping efficiency. a subset of the probes used in this study wa ... | 1994 | 7896104 |
| expression of functional proliferating-cell nuclear antigen from rice (oryza sativa) in escherichia coli. activity in association with human dna polymerase delta. | proliferating-cell nuclear antigen (pcna), the auxiliary protein for dna polymerase delta, is one of the key factors for both pcna-dependent dna synthesis and cell-cycle progression. plant pcna genes have previously been cloned from rice, carrot, tobacco, and soybean cells by screening the cdna libraries using similarity to the human or rat pcna genes. we subcloned the relevant gene from the rice pcna cdna into an escherichia coli expression vector pmal, and the pcna protein was expressed in the ... | 1994 | 7913441 |
| a unique sequence located downstream from the rice mitochondrial atp6 may cause male sterility. | asymmetric cell-fusion of the japonica cultivar of oryza sativa (rice) with cytoplasmic-male-sterile (cms) plants bearing cytoplasm derived from chinsurah boro ii, resulted in two classes of cytoplasmic hybrids (cybrids), fertile and cms. southern-blot analysis of the mitochondrial dna (mtdna) indicates recombination events around a number of genes; however, the appearance of the cms character is tightly correlated to reorganization around the atp6 gene, suggesting recombination downstream from ... | 1994 | 7915966 |
| efficient transformation of rice (oryza sativa l.) mediated by agrobacterium and sequence analysis of the boundaries of the t-dna. | a large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with agrobacterium tumefaciens. the efficiency of transformation was similar to that obtained by the methods used routinely for transformation of dicotyledons with the bacterium. stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transformants in the r0, r1 and r2 generations. sequence analysis revealed that the ... | 1994 | 7920717 |
| on the identification of group ii introns in nucleotide sequence data. | four different consensus sequences (gti, group ii identifiers) have been derived from domains v of known group ii introns and are used as query input sequences for sensitive database screenings with the fasta and lfasta programs. the set of four gti sequences can identify all domains v of the 96 known group ii introns in the completely sequenced chloroplast genomes of marchantia polymorpha, epifagus virginiana, oryza sativa, nicotiana tabacum and the completely sequenced mitochondrial genomes of ... | 1994 | 7932698 |
| induction of chilling resistance by water stress, and cdna sequence analysis and expression of water stress-regulated genes in rice. | exposure of seedlings of a chilling-sensitive variety of rice (oryza sativa l. cv. wasetoittu) to water stress (0.5 m mannitol, 30 min) at room temperature induced a degree of chilling resistance. no such resistance was induced by exogenous abscisic acid (aba) application (10 microm, 60 min). upon short-term water stress, new transcripts were expressed in both seedlings and suspension-cultured cells. we suggest that the genes induced by short-term water stress, and not those induced by aba, are ... | 1994 | 7948880 |
| transcribed repetitive dna sequences in telomeric regions of rice (oryza sativa). | we have isolated and characterized from rice three repetitive dna sequences, os48, osc-567, and osg3-430. our results indicate that these repetitive sequences are highly transcribed, and transcripts complementary to both strands of the os48 family of sequences account for up to 3% of the total cellular rna. pulsed-field gel electrophoresis, restriction mapping, and dna sequence analyses have revealed a complex pattern of structural organization of the three families of repetitive sequences. os48 ... | 1994 | 7948882 |
| blocking of anaerobic protein synthesis destabilizes dramatically plant mitochondrial membrane ultrastructure. | to elucidate the role of "anaerobic proteins" synthesized in plant cell under anoxia, the synthesis of these proteins was inhibited in rice (oryza sativa l.) coleoptiles and leaves by cycloheximide in the course of their anaerobic incubation. electron microscopic investigations demonstrated that the blockade of protein synthesis destabilized dramatically mitochondrial membrane fine structure. data obtained testify to significant role of anoxic stress proteins in the plant cell adaptation and sur ... | 1994 | 7951058 |
| partial purification and characterization of invertase isozymes from rice grains (oryza sativa). | four invertase isozymes have been isolated from the milky stage rice grains. according to the ph optima, they are classified as one alkaline (it7) and three acid invertases. the acid invertases are further divided into two soluble forms (it4 and it5) and one cell wall-bound (itb) form which was solubilized in 1 m nacl. the ph optima of itb, it4, it5 and it7 are 4.5, 3.5-4.0, 5.0 and 7.0, and the molecular masses are 42, 60, 64 and 260 kda, respectively. both it4 and it5 were bound to con a-sepha ... | 1994 | 7951078 |
| a likelihood approach for comparing synonymous and nonsynonymous nucleotide substitution rates, with application to the chloroplast genome. | a model of dna sequence evolution applicable to coding regions is presented. this represents the first evolutionary model that accounts for dependencies among nucleotides within a codon. the model uses the codon, as opposed to the nucleotide, as the unit of evolution, and is parameterized in terms of synonymous and nonsynonymous nucleotide substitution rates. one of the model's advantages over those used in methods for estimating synonymous and nonsynonymous substitution rates is that it complet ... | 1994 | 7968485 |
| organ-specific and hormone-dependent expression of genes for serine carboxypeptidases during development and following germination of rice grains. | several cdna clones encoding either serine carboxypeptidases or related proteins of oryza sativa l. were identified, and the abundance of the corresponding mrna in immature and germinated grains was examined. the deduced amino acid sequence of each cdna included key sequences, such as a pentapeptide (g-x-s-x-g/a) that is conserved among many serine carboxypeptidases, and the putative protein products were classified as two general and one novel type of cereal serine carboxypeptidases. two genera ... | 1994 | 7972496 |
| a group of chromosomal proteins is specifically released by spermine and loses dna-binding activity upon phosphorylation. | biologically relevant concentrations as low as 500 microm spermine led to the specific release of chromatin-associated proteins from nuclei of rice (oryza sativa) seedlings. using a southwestern technique, it was shown that several of these proteins bind dna. this affinity was lost upon in organello phosphorylation by an endogenous kinase. the effect of spermine was very specific. spermidine was far less effective and putrescine was essentially ineffective in releasing these proteins. the most a ... | 1994 | 7991684 |
| sequence of a cdna from oryza sativa (l.) encoding the pyruvate decarboxylase 1 gene. | 1994 | 7991697 | |
| protein phosphatase inhibitors enhance the expression of an alpha-amylase gene, alpha amy3, in cultured rice cells. | a rice (oryza sativa l.) gene for alpha-amylase, alpha amy3, was strongly and rapidly induced by treatment of suspension-cultured cells with okadaic acid (oa), a potent and specific inhibitor of protein serine/threonine phosphatases 1 and 2a. the massive accumulation of alpha amy3 mrna in response to oa treatment was due to the stimulation of gene transcription and a partial stabilization of this mrna. this induction of alpha amy3 message by oa occurred even though cellular protein synthesis was ... | 1994 | 7999117 |
| molecular cloning and biochemical characterization of a receptor-like serine/threonine kinase from rice. | a receptor-like protein kinase, ospk10, has been cloned from rice (oryza sativa). the 2.8 kb cdna contains an open reading frame capable of encoding a peptide sequence of 824 amino acids. the topological features of the predicted ospk10 protein include an n-terminal signal peptide, a cysteine-rich extracellular ligand-binding domain, a membrane-spanning segment, and a cytoplasmic domain possessing all the hallmarks of catalytic domains of eukaryotic protein kinases. the cytoplasmic domain was se ... | 1994 | 7999995 |
| molecular changes of organelle dna sequences in rice through dedifferentiation, long-term culture, or the morphogenesis process. | callus-specific rearranged dna in rice (oryza sativa l.) was isolated by in-gel reassociation procedure. southern hybridization experiments revealed that some clones were amplified significantly in primary callus induced from scutellum tissue. rapid amplification of these clones was observed within 2 days after plating seeds onto callus-induction medium containing 2,4-d. naa gave no significant effect on dna amplification event. colony formation process from isolated protoplasts and plant regene ... | 1994 | 8000003 |
| hydroxymethyl-phytochelatins [(gamma-glutamylcysteine)n-serine] are metal-induced peptides of the poaceae. | exposure of several species of the family poaceae to cadmium results in the formation of metal-induced peptides of the general structure (gamma-glu-cys)n-ser (n=2-4). they are assumed to be formed from hydroxymethyl-glutathione (gamma-glu-cys-ser) and are termed hydroxymethyl-phytochelatins (hm-pcs) in analogy to the homo-phytochelatins [(gamma-glu-cys)n-beta-ala], discovered in legumes, and the phytochelatins [pcs, (gamma-glu-cys)n-gly] found in most other plants and many fungi. the hm-pcs were ... | 1994 | 8016264 |