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influence of sampling time and diet on amino acid composition of protozoal and bacterial fractions from bovine ruminal contents.four ruminally cannulated cows were used to compare amino acid (aa) composition of protozoal and bacterial fractions as affected by sampling time and diet. cows were given once a day restricted feed (80% of ad libitum intake) of 7 kg dm with two successive diets. diet hb was 65% cocksfoot hay and 35% pelleted ground barley, and diet h was 100% cocksfoot hay. samples of whole ruminal contents were taken 2, 5, 8, 11, and 23 h after feeding for diet hb and 2 h after feeding for diet h to isolate th ...19968726749
[sobemovirus genome from dactylis glomerata has a luteovirus-like organization of the replicase and protease gene modules]. 20088754004
influence of barley and buffer supplements on quantitative aspects of ruminal fiber digestion of cows.four jersey cows, fitted with ruminal and duodenal cannulas, were used to assess the specific effects of changes in ruminal ph that were induced by barley addition to a hay diet on ruminal fiber digestion. cows received successively 100% unchopped cocksfoot hay (diet h), 65% hay and 35% pelleted ground barley (diet hb), and hb plus a continuous intraruminal infusion of bicarbonate salt solution (diet hbb). cows were limit-fed once daily at 7 kg of dm day-1. the ruminal fractional passage rate of ...19968767054
size-exclusion liquid chromatography and capillary electrophoresis of pollen allergens.allergens from the pollen of phleum pratense, dactylis glomerata. arrhenatherum elatius, secale cereale, lolium perrene and festuca sp. were analysed by size-exclusion chromatography (sec) and capillary electrophoresis (ce). sec was used for the determination of the molecular masses of main allergens. a ce method, using either 150 mmol/l phosphoric acid (ph 1.8) or a micellar system consisting of 50 mmol/l sodium dodecyl sulphate-20 mmol/l borate (ph 9.35), was developed as a rapid and efficient ...19968798911
cloning, sequencing and immunological characterization of dac g 3, a major allergen from dactylis glomerata pollen.preliminary work showed that a 14-kda allergen with a pi of 9 was recognized by more than 60% of sera from dactylis glomerata (dac g) pollen-allergic individuals. the n-terminal amino acid sequence of this dac g allergen was determined by edman degradation and compared with that of lol p 3, a major allergen of lolium perenne. a sequence identity of 65% was found, suggesting that the dac g allergen could be the homologue of lol p 3 and therefore named dac g 3. we report the cloning and sequence a ...19968811075
[olive pollinosis in japan].olive (olea europaes) is the most important allergic tree in the mediterranean area. that is widely distributed at syodoshima in japan. i observed olive pollination period and studied the patients with pollinosis. the patients were examined by skin test, measuring of specific ige antibody and nasal provocation test with olive pollen extract. allergic responses to olive pollen were found to be present in 16.3% of pollinosis patients. and cross-reactivity between olive and grass pollen (dactylis g ...19958857110
determination of sensitivity of inhalant allergens in patients with allergic rhinitis in west athens.in this study we have determined the sensitivity to inhalant (pollen and non-pollen) allergens in patients with allergic rhinitis from west athens, greece. the frequency of spt positivity was highest for dactylis glomerata, parietaria, olea europea, dermatophagoides and several moulds.19958919218
isotypic analysis of grass pollen-specific antibodies in human plasma. 4. biological activity of allergen-specific and autoanti-ige antibody fractions on basophil histamine release.blocking antibodies are defined as antibodies that compete with ige for binding to allergens due to their specificity for those allergens. thus, they may inhibit allergen-induced basophil and mast cell ige-dependent mediator release both in vivo and in vitro.19968955580
characterization of dac g 4, a major basic allergen from dactylis glomerata pollen.monoclonal antibodies were produced against dac g 4, a purified major basic allergen from dactylis glomerata pollen. their ability to be used for immunopurification of dac g 4 was studied on a biacore apparatus (pharmacia). the allergen was purified by affinity chromatography with one monoclonal antibody. its precise molecular mass, 59,185 +/- 30 d, was determined by mass spectrometry. its isoelectric point is 10.4. sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting show ...19968977507
an immunoblotting analysis of cross-reactivity between melon, and plantago and grass pollens.it is known that most patients with type i allergy to pollens also suffer intolerance to fruits. recently, an epidemiological and cap-inhibition study has shown a new clustering of allergy between melon and plantago and grass pollens. the aim of the present study was to confirm these results by immunoblotting analysis and inhibition of immunoblotting. sera from 3 patients with confirmed allergy to melon, and dactylis glomerata and plantago lanceolata pollens were used for the in vitro studies. s ...20139015782
kinetics of cell-wall digestion of orchardgrass and alfalfa silages treated with cellulase and formic acid.the objectives of this study were to determine the effects of cellulase (from trichoderma longibrachiatum) combined with formic acid, applied before ensiling, on the subsequent concentration and composition of the cell wall and on the extent and rate of in situ cell-wall digestion of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.). treated and control forages of both plant species were ensiled for at least 60 d before being ruminally digested by two fistulated cows. analyse ...19969029359
efficacy of intranasally applied dimethindene maleate solution as spray in adult volunteers with symptoms of seasonal allergic rhinitis in the vienna challenge chamber.twelve asymptomatic subjects (4 female, 8 male), being allergic to grass pollen proved by positive anamnesis, positive prick-test and positive nasal provocation test, were challenged under controlled conditions with purified airborne grass pollen of dactylis glomerata in the vienna challenge chamber (vcc), located at the universitätsklinik für hals-nasen-ohrenheilkunde, allgemeines krankenhaus (akh), der stadt wien, vienna (austria) by means of a double-blind, randomised, cross-over design, with ...19969125281
cross-reactivity to olive tree pollen and orchard grass pollen in patients with pollinosis.we studied 92 patients with allergic rhinitis in syodoshima, japan, during the pollen season between april and june to evaluate the cross-reactivity to different antigens, including pollen from the olive tree (olea europaea) and orchard grass (dactylis glomerata). olive tree pollen was present in the atmosphere for 23 days, from may 19 to june 12, 1994. specific ige antibodies for olive tree pollen antigen were present in 21 (26.9%) of the 78 patients with allergic rhinitis. nine (24.3%) of the ...19979227797
gramineae pollen as trigger factors of atopic eczema: evaluation of diagnostic measures using the atopy patch test.after contact with grass pollen, seasonal exacerbations of eczematous skin lesions have been described in a subgroup of patients with atopic eczema (ae). epicutaneous patch testing with aeroallergens (atopy patch test. apt) has been used to investigate these patients. we performed comparative apt in 79 patients with ae and 20 control subjects (14 non-atopic volunteers and six patients with grass pollen allergic rhinoconjunctivitis). subjects were tested with grass pollen allergen extract in petr ...19979292067
ruminal undegradability of blood meal and effects of blood meal on ruminal and postruminal digestion in steers consuming vegetative orchardgrass hay.four crossbred steers (360 +/- 3 kg) cannulated at the rumen and duodenum were used in a 4 x 4 latin square design to examine the effects of supplemental blood meal (bm) on voluntary intake, digesta kinetics, ruminal fermentation, site and extent of digestion, and bacterial protein synthesis in steers fed vegetative orchardgrass hay (dactylis glomerata l.). the levels of bm supplementation were 0, .07, .13, and .20 kg/d. voluntary intake of om (8.35 kg/d) was not significantly affected by bm sup ...19979331885
genetic structure of an aphid studied using microsatellites: cyclic parthenogenesis, differentiated lineages and host specialization.in a previous study, samples of the grain aphid sitobion avenae (f.) were collected from wheat and adjacent cocksfoot hosts in a population thought to be primarily parthenogenetic, and dna from individual aphids was analysed with a multilocus technique. here we have applied single-locus microsatellites and a mitochondrial dna marker to a subset of the same dna extracts, and have made several additional inferences about important genetic and population processes in s. avenae. microsatellite analy ...19979394464
evaluation of a mobile computerized grain feeder for lactating cows grazing grass pasture.the objective of this study was to evaluate a mobile computerized grain feeder for use to feed individually holstein cows grazing grass pasture. thirty-two holstein cows averaging 95 d of lactation and 39.3 kg/d of milk were rotationally grazed on predominantly dactylis glomerata pastures for 9 wk starting in early may. cows were blocked according to parity, days of lactation, and milk yield. cows were randomly assigned to a control group in which cows were individually fed grain twice daily at ...19979436109
effects of dietary supplementation on nutrient digestion and the milk yield of intensively grazed lactating dairy cows.fourteen midlactation holstein cows were used in an 80-d study to examine supplementation strategies during intensive rotational grazing. factors examined were the concentration of protein in the supplement [12 or 16% crude protein (cp) on a dry matter basis] and the amount of supplement offered (6.4 or 9.6 kg/d per cow). the supplement was offered in equal portions three times daily after milking. pasture, composed of alfalfa (medicago sativa) and orchardgrass (dactylis glomerata), was divided ...19979436110
variability in the pattern of random amplified polymorphic dna.the random amplified polymorphic dna (rapd) technique is a simple method to detect dna polymorphism. it is sensitive to reaction conditions. small changes in the reactants' concentration cause variations in amplification products. using dna from asparagus officinalis, dactylis glomerata, mercurialis annua and escherichia coli, we examined variability in the amplification pattern associated with reaction constituents. an increase in the ratio of taq dna polymerase to dna in the reaction increased ...19979504820
effect of extreme walking conditions for dairy cows on milk yield, chemical composition, and somatic cell count.thirty-two cows (16 montbeliardes and 16 tarentaises) in midlactation were used in an experiment utilizing a 2 x 2 factorial arrangement of treatments. throughout the trial, cows received first-cutting cocksfoot hay for ad libitum intake supplemented with a fixed amount of concentrate that was individually adapted to the milk yield of each cow. during a 23-d experimental period, one group of cows walked 9.6 km/d; the other group of cows remained in the barn. cows that walked daily ate less hay ( ...19989594388
effect of plant species on the larvae of gastrointestinal nematodes which parasitise sheep.faeces containing trichostrongylus colubriformis and/or ostertagia circumcincta eggs were used to provide four contaminations in each of 2 years on plots of browntop, yorkshire fog, ryegrass, tall fescue, lucerne, chicory, cocksfoot, white clover, and prairie grass and in the second year a mixed sward of ryegrass/white clover. third stage larvae were recovered from faeces and from four strata of herbage, 0-2.5, 2.5-5, 5-7.5 and > 7.5 cm above the soil surface at 2, 4, 6, 8, 11, and 14 weeks afte ...19989650060
detection and quantification of group 4 allergens in grass pollen extracts using monoclonal antibodies.grass pollen extracts are complex mixtures consisting of different major allergenic and non-allergenic components. phl p 4 is an important allergen, because more than 75% of grass pollen allergic patients produce specific ige antibodies against group 4 allergens.19989720813
how far can we simplify in vitro diagnostics for grass pollen allergy?: a study with 17 whole pollen extracts and purified natural and recombinant major allergens.current diagnostics for grass pollen allergy are composed of mixtures of pollen of different grass species. their complex composition hampers accurate standardization.19989723659
supplementation of rumen-undegradable protein to the diets of early lactation holstein cows on grass pasture.the objectives were to evaluate supplements differing in rumen-undegradable protein (rup) on the performance of early lactation cows on grass pasture. twenty-four holstein cows averaging 68 d of lactation and 39.8 kg/d of milk were rotationally grazed on predominantly dactylis glomerata pasture for 8 wk. cows were blocked according to parity, milk yield, and days of lactation and were randomly assigned to a grain mixture with a low or high rup content. pasture and the low and high rup grain mixt ...19989749386
[specific skin reactions induced by individual pollen preparations in hypersensitivity persons].skin tests are the most useful single modality for demonstrating an ige-mediated mechanism underlying clinical symptoms. skin reactivity to an allergen depends on person's exposure and genetic factors influencing the ige response. however, the reproducibility of allergy skin tests has been shown to be variable depending, among other things, on the allergen extract employed and the kind of technique used. these variations have led to controversy regarding clinical relevancy of allergy skin testin ...20099863408
environmental priming influences allergen-specific nasal reactivity.preceding mucosal response to one allergen leads to the priming of the nasal mucosal response to another allergen. this study aimed to determine whether environmental allergens, especially ubiquitous animal dander, can induce nasal priming.19989930593
physicochemical and immunologic characterization of low-molecular-weight allergoids of dactylis glomerata pollen proteins.orchard grass (dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins.199910221435
[antigen specific immunoglobulin e to grass and weed pollens in the plasma of patients with seasonal allergic rhinitis].the study involved 22 patients with seasonal allergic rhinitis between 13 and 53 years of age. the level of antigen specific ige (as ige) to 5 grass and 3 weed pollens was determined with the use of cap feia (pharmacia, uppsala, sweden). the control group consisted of 20 persons. all above as ige were significantly higher in the patients with seasonal allergic rhinithis than in the control group. the most commonly present hypersensitivities were to meadow fescue (festuca elatior), meadow grass ( ...199910337158
substitution of mainly lysyl groups of the proteins in an extract of dactylis glomerata by succinic and maleic anhydrides. 199910442536
inhibition of the seasonal ige increase to dactylis glomerata by daily sodium chloride nasal-sinus irrigation during the grass pollen season. 199910482855
identification of genes encoding for the cocksfoot mottle virus proteins.cocksfoot mottle sobemovirus (cfmv) has a monopartite single-stranded positive-sense rna genome. in wheat germ extract, in vitro translation of cfmv virion rna resulted in the production of four major proteins of 100, 71, 34 and 12 kda. in this paper we show the individual genes from which these polypeptides are synthesised. polyclonal antisera against the proteins encoded by all open reading frames (orfs) of cfmv were produced. antibodies were used to immunoprecipitate the in vitro translation ...199910486110
[in process citation]the phyllochron of cocksfoot (dactylis glomerata l.) was studied over a 6-week period with two levels of nitrogen supply (0 and 150 kg ha-1) and three cutting regimes which mimic lenient (p), severe (s) and continuous (c) grazing. the phyllochron was highest for treatment with n supply and it decreased from the p to the c defoliation regimes. during the study period, the phyllochron and the sheath length throughout the successive leaves appeared to increase, except for the c treatments. these pa ...199910505244
[investigations on the fermentation quality of silage from low-nitrate green forage].in 3-year-experiments green forage from dactylis glomerata and festuca species, produced by differentiated cutting frequencies, was ensiled under laboratory conditions. the forage was almost without nitrate. because of the higher content of water-soluble carbohydrates the fermentability coefficients of festuca species were above of dactylis glomerata. the fermentability coefficients of both plant species were inferior in the second and third cut, even if the first cut was delayed until july. des ...199910548978
chloroplast dna variation in dactylis glomerata l. taxa endemic to the macaronesian islandsin the dactylis glomerata infraspecific polyploid grass complex, restriction fragment length polymorphisms (rflps) of chloroplast dna (cpdna) were studied in diploid and tetraploid populations of several taxa endemic to macaronesia (madeira and the canary islands) and in populations from the african and european continental areas closest to macaronesia. two chlorotypes, which differed by a single 290-bp length mutation, were observed in the macaronesian and the continental populations. chlorotyp ...199910620224
consequences of phenotypic plasticity vs. interspecific differences in leaf and root traits for acquisition of aboveground and belowground resources.trade-offs between acquisition capacities for aboveground and belowground resources were investigated by studying the phenotypic plasticity of leaf and root traits in response to different irradiance levels at low nutrient supply. two congeneric grasses with contrasting light requirements, dactylis glomerata and d. polygama, were used. the aim was to analyze phenotypic covariation in components of leaf area and root length in response to above- and belowground resource limitation and the consequ ...200010719001
rna-binding activities of cocksfoot mottle sobemovirus proteins.cocksfoot mottle virus (cfmv) has a positive-sense ssrna genome containing four open reading frames (orfs). orf1 encoded protein (p1) is the putative movement protein; the product of orf2a (p2a) contains vpg and the motifs characteristic of serine proteases. p2b, encoded by orf2b, is the putative rna-dependent rna polymerase. p3, the coat protein, is encoded by orf3. cfmv p1, p2a, p2b, and p3, containing a six histidine tag at the amino terminus, were expressed in escherichia coli, purified and ...200010725552
[detection and characteristics of defective interfering rna associated with cocksfoot mottle sobemovirus]. 200810779963
secondary ion mass spectrometry imaging of the fixation of 15n-labelled no in pollen grains.we used secondary ion mass spectrometry to image cellular targets of nitrogen oxides (widespread air pollutants) in pollen grains of birch (betula verrucosa ehrh.) and cockfoot (dactylis glomerata l.). the pollen samples were exposed to air supplemented with high doses of 15no. the pollen grains were then fixed, dehydrated using a newly developed 'vapour phase' preparation method and embedded in lrw resin. semithin sections were then analysed. imaging was performed in scanning mode. as usual, th ...200010810006
regulation of -1 ribosomal frameshifting directed by cocksfoot mottle sobemovirus genome.the polyprotein of cocksfoot mottle virus (cfmv) is encoded by two overlapping open reading frames (orfs). the putative replicase of cfmv is produced as a part of the polyprotein from orf2b by the -1 ribosomal frameshifting mechanism. the signals leading to -1 ribosomal frameshifting directed by cfmv rna are the slippery heptamer uuuaaac and a stem-loop structure starting seven nucleotides downstream from the heptamer. we studied the effect of different parts of the cfmv genome on the -1 ribosom ...200010848968
effect of level of whole cottonseed on intake, digestibility, and performance of growing male goats fed hay-based diets.twenty-four purebred boer (capra hircus hircus) and 12 male kids of 1/2 boer breeding (initial bw 21 +/- .5 kg) were used in a randomized complete block design to study the effects of increasing level of whole cottonseed (wcs) on adg, serum urea n, plasma gossypol, live grades, and intake of dm, cp, ndf, adf, hemicellulose, cellulose, lignin, ether extract, and free gossypol. a subgroup of 16 purebred goats was used to determine digestibility using a 5-d total fecal collection. goats were blocke ...200010875652
enzyme, bacterial inoculant, and formic acid effects on silage composition of orchardgrass and alfalfa.we evaluated the effects of cellulase (from trichoderma longibrachiatum) application rates on neutral detergent fiber (ndf) concentration and fermentation products of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silages harvested with decreasing dry matter (dm) digestibility. additionally, the impacts of inoculant (lactobacillus plantarum and pediococcus cerevisiae), pectinase (from aspergillus niger), or formic acid on silage composition were studied. forages wilted to ...200010908057
a simple method to determine leaf angles of grass species.there are several very accurate methods to determine leaf angles in closed canopies. however, these are generally very time-consuming or require special equipment. average canopy leaf angles were derived from simple height and blade length measurements. an exponential relationship between the height/length ratio and the average blade leaf angle was used. the method was tested for two grass species, dactylis glomerata and festuca arundinacea, grown under different uv-b levels. the results clearly ...200010944161
respiratory costs and rate of protein turnover in the roots of a fast-growing (dactylis glomerata l.) and a slow-growing (festuca ovina l.) grass species.protein turnover is generally regarded as one of the most important maintenance processes in plants in terms of energy requirements. in this study, the contribution of protein turnover to the respiratory costs for maintenance in the roots of two grass species, the fast-growing d. actylis glomerata l. and the slow-growing f. estuca ovina l., is evaluated. plants were grown under controlled-environment conditions in a nutrient solution to which no(3)- was added at a relative addition rate of 0.2 a ...200010948236
characterization of vpg and the polyprotein processing of cocksfoot mottle virus (genus sobemovirus).the polyprotein of cocksfoot mottle virus (cfmv; genus sobemovirus:) is translated from two overlapping open reading frames (orfs) 2a and 2b by a -1 ribosomal frameshifting mechanism. in this study, a 12 kda protein was purified from viral rna-derived samples that appears to correspond to the cfmv genome-linked protein (vpg). according to the determined n-terminal amino acid sequence, the vpg domain is located between the serine proteinase and replicase motifs and the n terminus of vpg is cleave ...200011038392
intake, digestibility, and composition of orchardgrass and alfalfa silages treated with cellulase, inoculant, and formic acid fed to lambs.the objectives of this study were to determine the effect of a cellulase (from trichoderma longibrachiatum) alone or combined with a bacterial inoculant (lactobacillus plantarum and pediococcus cerevisiae) or formic acid on composition, intake, and digestibility of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silages. orchardgrass and alfalfa were harvested at the early heading stage and at the early bloom stage of maturity and wilted to approximately 22 and 32% dm, resp ...200011063325
[skin reactivity in the histamine-equivalent skin test in hypersensitivity to grass pollen].skin prick-test has been accepted as one of diagnostic criteria for atopic diseases. in accordance with the recommendations of the eaaci (european academy of allergy and clinical immunology), the subcommittee for skin testing, allergen specific skin prick-test should be estimated in relation to positive control (histamine hydrochloride in the hep system/histamine equivalent prick/) [1-4]. the purpose of this study was to establish a recording system of skin sensitivity to allergen by using hep m ...201311089421
cell-to-cell movement of potato virus x involves distinct functions of the coat protein.complementation of movement-deficient potato virus x (pvx) coat protein (cp) mutants, namely pvx.cp-xho lacking the 18 c-terminal amino acid residues and pvx.deltacp lacking the entire cp gene, was studied by transient co-expression with heterologous proteins. these data demonstrated that the potyvirus cps and both the major and minor cps of beet yellows closterovirus could complement cell-to-cell movement of pvx.cp-xho but not pvx.deltacp. these data also indicated that the c-terminally truncat ...200111161285
soil-soiln simulations of water drainage and nitrate nitrogen transport from soil core lysimeters.water resources protection from nitrate nitrogen (no3-n) contamination is an important public concern and a major national environmental issue. the abilities of the soil-soiln model to simulate water drainage and nitrate n fluxes from orchardgrass (dactylis glomerata l.) were evaluated using data from a 3-yr field experiment. the soil is classified as a hagerstown silt loam soil (fine, mixed, semiactive, mesic typic hapludalf). nitrate losses below the 1-m depth from n-fertilized grazed orchardg ...201311285920
fitting a regression model for genotype-by-environment data on heading dates in grasses by methods for nonlinear mixed models.the analysis of agricultural crop variety trials is usually complicated by the presence of genotype-by-environment interaction. a number of methods and models have been proposed to tackle this problem. one of the most common methods is the regression approach due to yates and cochran (1938, journal of agricultural science 28, 556-580), in which performances of genotypes in the environments are regressed onto environmental means. the underlying regression model contains a multiplicative term with ...199911315057
accumulation of metals in vegetation from an alkaline artificial soil.this research was conducted to determine whether elevated concentrations of metals in biosolids would result in increased accumulation of these metals in plants growing on an alkaline, artificial soil created by addition of municipal sewage biosolids to inorganic waste materials during reclamation of a filled lime settling basin. accumulation of metals in vegetation growing on this alkaline, artificial soil was compared with accumulation in vegetation growing on a natural, reference soil that ha ...200111381785
influence of low temperature preincubation on somatic embryogenesis and ethylene emanation from orchardgrass leaves.the objectives of this study were to determine the effects of low temperature (4 degrees c) preincubation on somatic embryogenesis from orchardgrass (dactylis glomerata l.) leaf cultures and to relate these effects to ethylene emanation during the preincubation and incubation periods. experiments were also conducted with an ethylene biosynthesis inhibitor aminooxyacetic acid (aoa). segments from the innermost two leaves were cultured on sh medium with 30 micromoles dicamba at 4 degrees c for 1 t ...199411537966
growth, proline accumulation and water relations of nacl-selected and non-selected callus lines of dactylis glomerata l.sodium chloride-tolerant calli were selected from leaf-derived embryogenic calli of dactylis glomerata l. on agar solidified medium supplemented with 200 mm nacl, a concentration lethal to non-selected calli. growth characteristics, water relations and proline accumulation pattern were compared in selected and non-selected lines. the objective was to gain an understanding of the mechanism(s) of tolerance in the nacl-tolerant line. growth in the selected line, as expressed in terms of tolerance i ...199511538458
spaceflight reduces somatic embryogenesis in orchardgrass (poaceae).somatic embryos initiate and develop from single mesophyll cells in in vitro cultured leaf segments of orchard-grass (dactylis glomerata l.). segments were plated at time periods ranging from 21 to 0.9 d (21 h) prior to launch on an 11 d spaceflight (sts-64). using a paired t-test, there was no significant difference in embryogenesis from preplating periods of 14 d and 21 d. however, embryogenesis was reduced by 70% in segments plated 21 h before launch and this treatment was significant at p=0. ...199811541444
ultrastructural analyses of somatic embryo initiation, development and polarity establishment from mesophyll cells of dactylis glomerata.somatic embryos initiate and develop directly from single mesophyll cells in in vitro-cultured leaf segments of orchardgrass (dactylis glomerata l.). embryogenic cells establish themselves in the predivision stage by formation of thicker cell walls and dense cytoplasm. electron microscopy observations for embryos ranging from the pre-cell-division stage to 20-cell proembryos confirm previous light microscopy studies showing a single cell origin. they also confirm that the first division is predo ...200811681324
growth in elevated co(2) can both increase and decrease photochemistry and photoinhibition of photosynthesis in a predictable manner. dactylis glomerata grown in two levels of nitrogen nutrition.biochemically based models of c(3) photosynthesis can be used to predict that when photosynthesis is limited by the amount of rubisco, increasing atmospheric co(2) partial pressure (pco(2)) will increase light-saturated linear electron flow through photosystem ii (j(t)). this is because the stimulation of electron flow to the photosynthetic carbon reduction cycle (j(c)) will be greater than the competitive suppression of electron flow to the photorespiratory carbon oxidation cycle (j(o)). where ...200111706199
poaceae in the natural diet of the snail helix aspersa müller (gastropoda, pulmonata).the natural diet of helix aspersa was studied in two populations by analysing faeces. picris echioides, carduus tenuifloris, urtica dioica, galium molugo (dicotyledons) and poaceae (monocotyledons) were the principal resources of the studied populations. the species of poaceae ingested by helix aspersa in july were determined by analysing the phytoliths present in the faeces. festuca rubra, dactylis glomerata and bromus hordeaceus seemed to be attractive for the snails whereas elytrigia repens w ...200111725705
immunochemical characterization of two pichia pastoris-derived recombinant group 5 dactylis glomerata isoallergens.grass pollen of the poaceae grasses are known to be highly allergenic. major allergens from the species lolium, phleum, poa and holcus have been cloned and expressed as recombinant proteins, but of the important species dactylis glomerata no recombinants are available.200111752876
species and nitrogen effect on growth rate, tiller density, and botanical composition in grass hay production.numerous studies have evaluated growth, stand dynamics, and botanical composition of grass swards separately, but little information is available on the interaction of these factors. the objectives of this study were to determine the response of growth rate, tiller density, and botanical composition in orchardgrass (dactylis glomerata l.), smooth bromegrass (bromus inermis leyss.), and timothy (phleum pratense l.) to different n levels. growth rates ranged from 8.6 to 1.54, 9.14 to 0.67, and 4.1 ...200211756276
a model of lamina digestibility of orchardgrass as influenced by nitrogen and defoliation.the morphogenesis of grass-sward regrowth drives the relationship between sward management effects and herbage digestibility. our objective was to create a model of herbage digestibility for a range of n fertilizer levels and defoliation practices on the basis of changes in vegetative grass tiller structure rather than on dates of cutting or grazing. an experiment was conducted for two spring and two summer regrowths to examine the digestibility of orchardgrass (dactylis glomerata l.) whole lami ...200211756277
seedling development and field performance of prairiegrass, grazing bromegrass, and orchardgrass.seedling establishment is a critical phase in pasture management. knowledge of the seedling development of new forages is necessary to develop management practices and recommend species mixtures for pasture seedings. we compared seedling growth and development of prairiegrass (bromus willdenowii kunth = b. catharticus vahl), grazing bromegrass (b. stamineus desv.), and orchardgrass (dactylis glomerata l.) in controlled environment and field studies. seedlings were sampled weekly for 7 wk in the ...200211756278
supplementation with partially hydrogenated oil in grazing dairy cows in early lactation.effects of partially hydrogenated oil on performance, loss of body weight and body condition score, and blood metabolite and hormone concentrations were evaluated in 37 multiparous holstein cows in grazing conditions during the first 100 d of lactation. six additional holstein cows, each fitted with a ruminal cannula, were allocated to a replicated 3 x 3 latin square to evaluate effects of supplemental fat on rumen environment and pasture digestion. all cows grazed mixed pastures based on alfalf ...200211949863
the contribution of roots and shoots to whole plant nitrate reduction in fast- and slow-growing grass species.the hypothesis was tested that slow-growing grass species perform a greater proportion of total plant no3- reduction in their roots than do fast-growing grasses. eight grass species were selected that differed in maximum relative growth rate (rgr) and net no3- uptake rate (nnur). plants were grown with free access to nutrients in hydroponics under controlled-environment conditions. the site of in vivo no3- reduction was assessed by combining in vivo no3- reductase activity (nra) assays with biom ...200212096102
the complete sequence of the genome of cocksfoot streak virus (csv), a grass infecting potyvirus.the complete nucleotide sequence of cocksfoot streak virus (csv) has been determined. the viral genome comprises 9663 nucleotides, excluding a 3'-terminal poly(a) sequence. the genome of csv has a 133 nt 5'-non coding and a 260 nt 3'-non coding region. the rna of csv encodes a single polyprotein of 3089 amino acid residues and has a deduced genome organization typical for a member of the family potyviridae. csv is transmissible by aphids and has a narrow host range in the gramineae. it was compa ...200212181676
drought survival, summer dormancy and dehydrin accumulation in contrasting cultivars of dactylis glomerata.to study survival under prolonged and severe drought in the perennial grass dactylis glomerata we compared dormant, resistant and sensitive cultivars (cvs.) in both field and glasshouse experiments. water status, membrane stability and expression of dehydrins were assessed in the immature leaf bases, which are the last surviving organs. analysis of leaf elongation and senescence of aerial tissues showed that dormancy was exhibited by the potentially dormant cultivar (cv.) only in the field. this ...200212207661
inhibition of sucrose:sucrose fructosyl transferase by cations and ionic strength.fructans are storage carbohydrates found in many temperate grasses. the first enzyme in the biosynthetic pathway of most fructans is sucrose:sucrose fructosyl transferase (sst). in this report, we demonstrate that k+ and ionic strength noncompetitively inhibit the activity of sst from wheat (triticum aestivum l.) stems. the ki for this inhibition is high, 122 mm, but in the range of concentrations of k+ found in the tissue (205-314 mm). addition of kcl to the assay system had no effect on the ph ...199312231714
roles of the plasma membrane and the cell wall in the responses of plant cells to freezing.in an effort to clarify the responses of a wide range of plant cells to freezing, we examined the responses to freezing of the cells of chilling-sensitive and chilling-resistant tropical and subtropical plants. among the cells of the plants that we examined, those of african violet ( saintpaulia grotei engl.) leaves were most chilling-sensitive, those of hypocotyls in mungbean [ vigna radiata (l.) r. wilcz.] seedlings were moderately chilling-sensitive, and those of orchid [ paphiopedilum insign ...200212244442
a new attempt to study biomineralised silica bodies in dactylis glomerata l.the accumulation process of inorganic compounds in animals and plants by biomineralisation is not well understood nowadays, though it may be the key to an environmental-compatible production of modern materials in future. in this paper a new attempt will be made on the investigation of silica accumulation in grasses (especially dactylis glomerata l.). the silicic acid agglomerates in dactylis glomerata l. were studied by means of scanning electron microscopy in combination with energy dispersive ...200212397507
effects of elevated soil copper on phenology, growth and reproduction of five ruderal plant species.the repeated use of copper (cu) fungicides to control vine downy mildew, caused by plasmopara viticola, has been responsible for the heavy increase of cu concentration in the upper layers of vineyard soils. to determine the effects of elevated soil cu on plant development, we created an artificial soil gradient with cu enrichments ranging from 0 to 400 mg kg-1. on this gradient, and for five ruderal plant species commonly found in vineyards in southern france (poa annua l., dactylis glomerata l. ...200312547525
effect of smoke, charred wood, and nitrogenous compounds on seed germination of ten species from woodland in central-western spain.the effect of smoke, charred wood, and nitrogenous compounds on germination was tested on 10 species of the cistaceae, poaceae, fabaceae, and asteraceae, from fire-prone, shrubby woodlands in central-western spain. dry seeds were exposed to smoke, by watering with distilled water-charred wood suspensions, or nano2, kno3, nh4cl, and nh4no3. smoke enhanced germination in 9 of 10 of the species. in species of poaceae, germination was stimulated by 20 min of smoke exposure. in asteraceae and fabacea ...200312647865
transpiration rates and canopy conductance of pinus radiata growing with different pasture understories in agroforestry systems.we measured tree transpiration and canopy conductance in pinus radiata d. don at two low rainfall sites of differing soil fertility in canterbury, new zealand. at the more fertile lincoln site, we also assessed the effects of two common pasture grasses on tree transpiration and canopy conductance. at the less fertile eyrewell forest site, the effect of no understory, and the effects of irrigation in combination with mixtures of grass or legume species were determined. tree xylem sap flux (f(d)') ...199812651344
silica accumulation in triticum aestivum l. and dactylis glomerata l.the silica accumulation in orchard grass (dactylis glomerata l.) and wheat (triticum aestivum l.) has been studied in plant samples grown under defined conditions in a pot trial. the plant habit and the quantity of biomineralised silica within the selected gramineae depend to a remarkable extent on the soil. the plants grew with different soil ph values and silica additives. by means of atomic absorption spectrometry, the silicon enrichment in different plant parts was determined. in dried plant ...200312698227
supplemental carbohydrate sources for lactating dairy cows on pasture.two experiments were conducted to evaluate steam-flaked corn and nonforage fiber sources as supplemental carbohydrates for lactating dairy cows on pasture. cows were allotted to a new paddock of an orchardgrass (dactylis glomerata l.) pasture twice daily in one group in both trials. in experiment 1, 28 holstein cows, averaging 216 d in milk, were randomly assigned to either a cracked-corn (cc) or a steam-flaked (sfc) supplement in a split plot design. the supplement contained 66.7% of corn and a ...200312703627
oxygen deficiency affects carbohydrate reserves in overwintering forage crops.anaerobic conditions developing under an ice cover affect winter survival and spring regrowth of economically important perennial crops. the objective was to compare, during a prolonged period of low (<2%) o2 at low temperature, the concentration of carbohydrates of four plant species contrasting in their resistance to oxygen deficiency. four perennial forage species, lucerne (medicago sativa l.), red clover (trifolium pratense l.), timothy (phleum pratense l.), and cocksfoot (dactylis glomerata ...200312754268
the three-dimensional structure of cocksfoot mottle virus at 2.7 a resolution.cocksfoot mottle virus is a plant virus that belongs to the genus sobemovirus. the structure of the virus has been determined at 2.7 a resolution. the icosahedral capsid has t = 3 quasisymmetry and 180 copies of the coat protein. except for a couple of stacked bases, the viral rna is not visible in the electron density map. the coat protein has a jelly-roll beta-sandwich fold and its conformation is very similar to that of other sobemoviruses and tobacco necrosis virus. the n-terminal arm of one ...200312781716
effect of soil type and fertilization level on mineral concentration of pasture: potential relationships to ruminant performance and health.a three-year study was conducted to measure the effects of varying levels of dairy slurry application on mineral concentration of forages from three soils types. slurry was applied to orchardgrass (dactylis glomerata [l.] cv. pennlate) growing in 60-cm diameter drainage lysimeters to measure the effect of four levels of slurry (urine and feces) n application (0, 168, 336, and 672 kg of n.ha-1.yr-1) on mineral (p, k, ca, mg) concentration of the forage on three soil types (hagerstown, hartleton, ...200312817509
[in vitro effects of antiallergic eyedrops on complement activation induced by particulate matter].recent decades have been marked by an increasing number of patients suffering from ocular allergic-like symptoms without being associated with an increase in ige levels. these symptoms include heaviness of the lid, foreign body sensation, burning, stinging and photophobia. both epidemiological studies and controlled human exposure clinical studies have shown cause-effect relationships between allergic-like symptoms and environmental factors such as outdoor air pollutants or poor indoor air quali ...200312843888
soil mixing to decrease surface stratification of phosphorus in manured soils.continual applications of fertilizer and manure to permanent grassland or no-till soils can lead to an accumulation of p at the surface, which in turn increases the potential for p loss in overland flow. to investigate the feasibility of redistributing surface stratified p within the soil profile by plowing, mehlich-3 p rich surface soils (128-961 mg kg(-) in 0-5 cm) were incubated with lower-p subsoil (16-119 mg kg(-1) in 5-20 cm) for 18 manured soils from oklahoma and pennsylvania that had rec ...200312931893
vertical leaf nitrogen distribution in relation to nitrogen status in grassland plants.vertical gradients of leaf nitrogen (n) per unit leaf area (nla) are viewed as plastic responses that optimize n utilization with respect to carbon assimilation. however, it has been shown that plant species, sowing density and n availability affect the steepness of the nla gradient relative to the photon flux density (pfd) gradient. this paper tests the hypothesis that such variation is related to the n status of the plant. the n status was analysed using the concept of the critical n concentra ...200312967909
comparison of growth performance of lolium perenne l., dactylis glomerata l. and agropyron elongatum (host.) p. beauv. for erosion control in turkey.this study was carried out in plastic containers to compare growth performances of perennial ryegrass, orchardgrass and tall wheatgrass to be given priority in revegetation studies in turkey. three pre-germinated seeds of each grass species were planted separately into the soil in the black plastic containers. seedlings were harvested 2, 4 and 6 months after planting pre-germinated seeds and measured for percentage of seedling emergence, rooting depth, height growth, leaf and tiller development ...200312974411
chlorophyll lethal in natural populations of the orchard grass (dactylis glomerata l.). a case of balanced polymorphism in plants. 196113683843
grass pollen allergens. iv. the isolation of some of the principal allergens of phleum pratense and dactylis glomerata and their sensitivity spectra in patients. 196213863259
human serum ige-mediated mast cell degranulation shows poor correlation to allergen-specific ige content.although allergen-specific ige content in serum can be determined immunochemically, little is known about the relationship between this parameter and the strength of the degranulation response upon allergen triggering.200314510723
the complete nucleotide sequence of subterranean clover mottle virus.the complete nucleotide sequence of subterranean clover mottle virus (scmov) genomic rna has been determined. the scmov genome is 4,258 nucleotides in length. it shares most nucleotide and amino acid sequence identity with the genome of lucerne transient streak virus (ltsv). scmov rna encodes four overlapping open reading frames and has a genome organisation similar to that of cocksfoot mottle virus (cfmv). orf1 and orf4 are predicted to encode single proteins. orf2 is predicted to encode two pr ...200314579180
[effects of grazing, cutting and decapitating on grass populations on the artificial grassland in subtropical zone of china].studies on the effects of grazing, cutting and decapitating on grass populations on the artificial grassland in the subtropical zone of china showed that moderate grazing and cutting could increase the adaptability of dactylis glomerata and lolium prenn due to decapitating. decapitating could rescind the apical dominance and reproduction growth of grass, and hence, the ecological effects of grazing and cutting on grass could be realized by decapitating, and the population density, caloric value ...200314655369
the effect of five forage species on transport and transformation of atrazine and isoxaflutole (balance) in lysimeter leachate.a field lysimeter study with bare ground and five different ground covers was established to evaluate the effect of forage grasses on the fate and transport of two herbicides in leachate. the herbicides were atrazine (atr; 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) and isoxaflutole [ixf; 5-cyclopropyl-4-(2-methylsulfonyl-4-trifluormethyl-benzoyl)isoxazole], which has the commercial name balance (aventis crop science, strasbourg, france). the ground covers included orchardgrass (dacty ...201314674520
inferring the evolutionary history of rice yellow mottle virus from genomic, phylogenetic, and phylogeographic studies.fourteen isolates of rice yellow mottle virus (rymv) were selected as representative of the genetic variability of the virus in africa from a total set of 320 isolates serologically typed or partially sequenced. the 14 isolates were fully sequenced and analyzed together with two previously reported sequences. rymv had a genomic organization similar to that of cocksfoot mottle sobemovirus. the average nucleotide diversity among the 16 isolates of rymv was 7%, and the maximum diversity between any ...200415016846
vegetation establishment on a deposit of zinc mine wastes.field trials concerning the establishment of plant cover on a deposit of wastes from the ammeberg zinc mine in central sweden were carried out during 1976-1985. different soil conditioners and manures were applied and plant species cultivars were evaluated with regard to plant biomass, vigour, durability and content of zinc, lead and cadmium. sewage sludge and topsoil led to better establishment of grasses than did municipal waste, straw and hydraulic seeding. after 2 years, festuca rubra and po ...198915092483
effects of ambient levels of air pollution on grass swards subjected to different defoliation regimes.the urgent need to evaluate effects of air quality on plant growth in the field is discussed in relation to the difficulties which field-based studies pose in the interpretation of data. an initial study is described which aimed to overcome some of these difficulties and the data are discussed within the remaining limitations. the productivity of pure swards of lolium perenne l. and dactylis glomerata l., subjected to defoliation at different times of the year, was studied at field sites with di ...198715092760
effect of manure application timing, crop, and soil type on phosphorus leaching.phosphorus (p) leaching losses from manure applications may be of concern when artificial drainage systems allow for hydrologic short-cuts to surface waters. this study quantified p leaching losses from liquid manure applications on two soil textural extremes, a clay loam and loamy sand soil, as affected by cropping system and timing of application. for each soil type, manure was applied at an annual rate of 93 800 l ha(-1) on replicated drained plots under maize (zea mays l.) in early fall, lat ...201315224946
analysis of liquid extracts from tree and grass pollens by capillary electromigration methods.capillary electromigration methods, zone electrophoresis (cze), micellar electrokinetic chromatography (cmekc) and isotachophoresis (citp), have been used for analysis of water and water-buffer extracts from tree-common birch (betula verrucosa) and grass-orchardgrass (dactylis glomerata) pollen samples. water extracts were analyzed by cze using acetic acid as background electrolyte (bge), by cmekc in tris-phosphate bge with anionic detergent sodium dodecyl sulfate (sds) micellar pseudophase (tp- ...200415236695
lactation performance of holstein cows fed fescue, orchardgrass, or alfalfa silage.perennial grasses are increasingly being used as an integral part of nutrient management plans, but fescue (festuca arundinacea schreb.) is often overlooked because of perceived intake problems. a 30-d study was conducted to evaluate the lactation performance of cows fed a fescue silage-based total mixed ration (tmr) compared with orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silage-based tmr, when forages are harvested at recommended neutral detergent fiber (ndf) levels. ...200415328241
characterization of natural dac g 1 variants: an alternative to recombinant group 1 allergens.production of soluble correctly folded recombinant group 1 allergens has proven to be difficult. purified natural group 1 allergens could be an alternative for application in immunotherapy.200415536420
myeloperoxidase release after allergen-specific conjunctival challenge.allergen-specific conjunctival challenge is a fruitful and complete tool in evaluating pathophysiological phenomena of allergic inflammation. after challenge, a significant neutrophil infiltrate occurred in allergic subjects. the primary (azurophilic) granules of neutrophils contain a variety of enzymes that might potentiate inflammation, such as myeloperoxidase (mpo). it is not known whether allergen-specific conjunctival challenge (ascc) is able to elicit mpo release. we also investigated the ...200415584313
surfactant protein d increases phagocytosis and aggregation of pollen-allergen starch granules.recent studies have shown that surfactant components, in particular the collectins surfactant protein (sp)-a and -d, modulate the phagocytosis of various pathogens by alveolar macrophages. this interaction might be important not only for the elimination of pathogens but also for the elimination of inhaled allergens and might explain anti-inflammatory effects of sp-a and sp-d in allergic airway inflammation. we investigated the effect of surfactant components on the phagocytosis of allergen-conta ...200415591410
[ecological strategies of soil microbial communities under plants of meadow ecosystems].dominant growth strategies of soil microbial communities of mown and unmown meadows were assessed with respect to the constants of saturation and maximal specific growth rate of microorganisms. the microbial community of mown-meadow soil was characterized by a greater biomass and activity due to prevalence of microorganisms with the r strategy, compared to the microbial community of unmown-meadow soil. in contrast to nonrhizosphere soil, rhizosphere soil was dominated by rapidly growing microorg ...200415615453
the major grass pollen group 5 allergen from dactylis glomerata and its c-terminal split product both behave as dimers: implications for allergen standardization.on sds-page grass pollen group-5 allergens migrate as a doublet with an apparent molecular mass (m(r)) of 25 kda. immunoblot analysis revealed additional group 5 reactivity at double and half this m(r). the aim of this study was to investigate these group 5 molecular entities and to compare their allergenicity and behavior in quantitative immunoassays.200515650308
seasonal patterns of growth, dehydrins and water-soluble carbohydrates in genotypes of dactylis glomerata varying in summer dormancy.summer dormancy in perennial grasses has been studied inadequately, despite its potential to enhance plant survival and persistence in mediterranean areas. the aim of the present work was to characterize summer dormancy and dehydration tolerance in two cultivars of dactylis glomerata (dormant 'kasbah', non-dormant 'oasis') and their hybrid using physiological indicators associated with these traits.200515760915
sensitization to common allergens, especially pollens, among children with respiratory allergy in the trakya region of turkey.asthma and allergic rhinitis are common problems in children and the causative pollen allergens vary according to the geographical area. the aim of this study was to investigate patterns of sensitization to common inhalant allergens, especially pollens, in turkish children living in the trakya region and to determine differences between rural and urban areas. allergen skin testing was prospectively performed on 539 children aged between 4 and 17 years with respiratory allergy. the reaction was c ...200415783130
[effects of grazing on architecture and small-scale pattern of grasses on artificial grassland in subtropical zone].this study was conducted on a 5-year artificial grassland in subtropical zone of south china. the main types of established artificial grassland there were dactylis glomerata-lolium prenne-trifolium repens and d. glomerata-t. repens pastures. four grazing intensities were designed, i.e., ck (no grazing), g1 (6 adult sheep x hm(-2)), g2 (7.5 adult sheep x hm(-2)) and g3 (10 adult sheep x hm(-2)), and all the grazing plots were rotationally grazed. the architecture and small-scale pattern of grass ...200415825439
ruminal in situ disappearance kinetics of dry matter and fiber in growing steers for common crabgrass forages sampled on seven dates in northern arkansas.southern crabgrass (digitaria ciliaris [retz.] koel.) is often viewed as an undesirable weed, largely because it encroaches upon field and forage crops, gardens, and lawns. however, visual observations of livestock grazing mixed-species pastures suggest that cattle seem to prefer crabgrass to many other summer forages. the objectives of this study were to assess the nutritive value of crabgrass sampled weekly between july 11, and august 22, 2001, and then to determine ruminal in situ disappearan ...200515827259
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