Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| lung injury by furanoterpenoids isolated from fusarium solani infected sweet potato, ipomea batatas. | intraperitoneal administration (1 mg/kg body/wt./day for 21 days) of crude extract of furanoterpenoids, isolated from f. solani damaged i. batatas caused pulmonary oedema in albino rats. the elevated broncho alveolar lavage (bal) angiotensin converting enzyme (ace), lactate dehydrogenase (ldh) and protein levels indicated lung damage. the estimation of pulmonary extracellular surfactant phospholipids showed an alteration in various phospholipid fractions. | 1993 | 8359843 |
| affinity purification of beta-amylases originating from plant using cyclomaltohexaose-immobilized sepharose 6b in the presence of ammonium sulfate. | this paper reports a novel method of affinity purification of soybean and barley beta-amylase on cyclomaltohexaose-immobilized sepharose. until now, it has been shown that sweet potato beta-amylase can be purified using the above absorbent but beta-amylases from soybean and barley seeds cannot. we found that soybean and barley beta-amylase becomes adsorbed specifically on the above absorbent if it is in solution with 1 to 2 m ammonium sulfate, and the adsorbed enzyme can be easily eluted with a ... | 1993 | 8374302 |
| sensory evaluation and nutrient composition of weaning food from pregelatinized maize-sweet potato mixtures. | pregelatinized maize-sweet potato mix, fortified with soybeans and groundnut flours, was evaluated for its acceptability as a weaning food and for nutrient composition. an acceptable product had 14.3, 6.4, 2.4 and 67.4%, respectively, for its protein, fat, ash and carbohydrate contents. values for calcium, phosphorus and and iron contents were 59.6, 187.5 and 2.4 mg/100 g, respectively, while the most predominant fatty acids were linoleic, oleic and palmitic acids. the product, which had a chemi ... | 1993 | 8378272 |
| cloning of the beta-amylase gene from bacillus cereus and characteristics of the primary structure of the enzyme. | the gene encoding the beta-amylase of bacillus cereus bq10-s1 (spoii) was cloned into escherichia coli jm 109. a sequenced dna fragment of 2,001 bp contains the beta-amylase gene. the n-terminal sequences (avngkg mnpdykaylmaplkki), the c-terminal sequences (shtssw), and the amino acid sequences of the five regions in the beta-amylase molecules were determined. the mature beta-amylase contains 514 amino acid residues with a molecular mass of 57,885 da. the amino acid sequence homology with those ... | 1993 | 8434930 |
| analysis of genetic diversity in a sweetpotato (ipomoea batatas) germplasm collection using dna amplification fingerprinting. | a dna amplification fingerprinting (daf) approach was employed to develop individual-specific profiles and analyze genetic relationships among 73 plant introductions of sweetpotato (ipomoea batatas (l.) lam.) including unadapted lines from around the world and a few selected u.s.a. cultivars. reliable and informative fingerprint profiles were obtained employing single octamer primers and stoffel fragment taq polymerase in the polymerase chain reaction, polyacrylamide-based vinyl polymer for elec ... | 1995 | 8537002 |
| o-glycosylation of a precursor to a sweet potato vacuolar protein, sporamin, expressed in tobacco cells. | sporamin, a vacuolar protein of the sweet potato, is synthesized as a precursor that contains signal peptide and an n-terminal propeptide that functions as a vacuolar targeting determinant. sporamin, when expressed in tobacco cells, migrated as smeared bands on an sds-polyacrylamide gel. the smearing was due to o-glycosylation of the precursor to sporamin. the smeared bands were stained by a glycan-specific stain but no n-glycosylation site was found in the amino acid sequence of the precursor t ... | 1995 | 8580960 |
| determination of thiabendazole residues in white and sweet potatoes by liquid chromatography with fluorescence detection. | a liquid chromatographic (lc) method was developed for determination of thiabendazole (tbz) residues in or on whole, unwashed white potatoes (solanum tuberosum) and sweet potatoes (ipomoea batatas). tbz is extracted from the potato homogenate with ethyl acetate and the extract purified and concentrated on a cation-exchange, solid-phase extraction column. the extract is analyzed for tbz residues by column lc with a cation-exchange column and fluorescence detection. recoveries of tbz from whole wh ... | 2013 | 8664582 |
| bombyxin-related peptides: cdna structure and expression in the brain of the hornworm agrius convolvuli [corrected]. | we have cloned three cdnas from the sweet potato hornworm agrius convolvuli that encode precursor molecules for peptides structurally related to bombyxin, an insulin-related brain secretory peptide in bombyx mori. the agrius bombyxin-related peptide (abrp) cdnas are classified into type a and b according to their sequence similarity. the prepro-abrps deduced from the cdna sequences have the insulin-like domain organization of signal peptide/b chain/c peptide/a chain. the abrp transcripts in agri ... | 1996 | 8673077 |
| interaction of a potential vacuolar targeting receptor with amino- and carboxyl-terminal targeting determinants. | a protein of 80 kd from developing pea (pisum sativum) cotyledons has previously been shown to exhibit characteristics of a vacuolar targeting receptor by means of its affinity for the amino-terminal vacuolar targeting sequence of proaleurain from barley (hordeum vulgare). in this report we show that the same protein also binds to the amino-terminal targeting peptide of prosporamin from sweet potato (ipomoea batatas) and to the carboxyl-terminal targeting determinant of pro-2s albumin from brazi ... | 1996 | 8787025 |
| replacement of maize with sweet potato (ipomoea batatas l.) tuber meal in broiler diets. | 1. the nutrient composition of sweet potato tuber meal (spm) was similar to that of maize, with the exceptions of crude protein and calcium. the crude protein content was lower (62 v 91 g/kg) and the calcium content was higher (l.9 v 0.l g/kg) in spm. the amino acid composition, in general, favoured spm. 2. trypsin inhibitor activity, and the contents of oxalate and phytate-phosphorus in spm were too low to be of any nutritional concern. 3. the apparent metabolisable energy content of spm was de ... | 1996 | 8833531 |
| effects of oven-drying tubers of two high-protein sweet potato varieties at different temperatures on their feeding value in broilers. | 1. unpeeled tubers from 2 high-protein varieties of sweet potato (white-fleshed bosbok, and orange-fleshed carmel) were chipped and oven-dried at 40, 60, or 80 degrees c, to examine the effects on nutritive value. the dried ground chips were substituted for maize at 500 g/kg and the diets fed to day-old, ross-1 broiler chicks for 3 weeks. 2. compared with carmel, bosbok had a lower crude protein content (90 v. 138 g/kg dm) and trypsin inhibitor activity (tia) (3 v. 5 mg of trypsin inhibited per ... | 1996 | 8833537 |
| application of sweet potato fiber to skin wound in rat. | in order to introduce a suitable drug mixing base or covering for burns or decubital wounds, the usefulness of fiber extracted from the sweet potato was investigated. the healing effect of the fiber was evaluated by examining the extent of reduction in the size of wounds and changes in the quality of wounds in rats. in contrast to the control, the use of the fiber alone as a wound covering material reduced wounded areas by 21.0% at postoperative day 9, 19.5% at day 11, and 18.7% at day 13, and r ... | 1996 | 8839973 |
| isolation, characterisation and cell growth-regulatory properties of kumara (sweet potato) trypsin inhibitors. | locally grown kumara (sweet potato; ipomea batatis) was used as the starting point for the purification of a proteinase inhibitor. the purified inhibitor was highly specific for trypsin, and much less effective as an inhibitor of chymotrypsin. two 22kda variants were present, closely homologous to each other and to sporamin a, but with a single amino acid substitution (proline in place of serine, the second residue in mature sporamin a). one variant had the same n-terminus as sporamin a, whereas ... | 1996 | 8850529 |
| ultraweak luminescence generated by sweet potato and fusarium oxysporum interactions associated with a defense response. | ultraweak luminescence generated by sweet potato and nonpathogenic fusarium oxysporum interactions associated with a defense response was detected by a photoncounting method using ultrahigh-sensitive photodetectors. the time-dependent intensity variation, the spectrum and the two-dimensional imaging of the ultraweak luminescence are indicative of the defense response of the sweet potato to f. oxysporum. the production of ipomeamarone as a phytoalexin means that f. oxysporum induced the defense r ... | 1996 | 8972637 |
| two acylated anthocyanins from purple sweet potato. | two new acylated anthocyanins were isolated from the storage root of ipomoea batatas. on the basis of spectroscopic analyses, these compounds were identified as the 3-o-(6-o-trans-caffeyl-2-o-beta-glucopyranosyl-beta-glucopyranoside)-5-o - beta-glucoside of cyanidin and peonidin. | 1997 | 8983218 |
| vitellin of the sweet potato whitefly, bemisia tabaci: biochemical characterization and titer changes in the adult. | sds-page of the sweet potato whitefly (bemisia tabaci) egg extract showed one major band (approximately 190 kda) and two minor bands (approximately 75 kda and 67 kda). a distinct 190 kda band was also present in male extract. on sds gels the vitellin band of the greenhouse whitefly (trialeurodes vaporarium) was larger, about 220 kda. the native molecular mass of sweet potato whitefly vitellin was estimated to be 375 kda using 4-20% native porelimiting gel electrophoresis. its isoelectric point w ... | 1997 | 9041698 |
| functional activity of sporamin from sweet potato (ipomoea batatas lam.): a tuber storage protein with trypsin inhibitory activity. | sporamin accounts for about 60% to 80% of total soluble protein in sweet potato tubers, and the predicted protein sequence of sporamin shares significant amino acid sequence identity with some kunitz-type trypsin inhibitors. we constructed three recombinant plasmids with cdnas that encode preprosporamin, prosporamin, and sporamin, and these three were expressed in escherichia coli cells as fusion proteins. all three forms of sporamin expressed in e. coli were shown to have strong inhibitory acti ... | 1997 | 9049277 |
| a cdna encoding a putative spf1-type dna-binding protein from cucumber. | a cdna clone encoding a polypeptide with homology to the novel spf1 dna-binding protein of sweet potato has been isolated from a cdna library from rna of senescing cucumber (cucumis sativus, l.) cotyledons. comparison of the two sequences reveals similar features which may be important in the evolution and function of this protein, including a duplicated region of about 56 amino acids (aa). the first half of the duplicated region is enriched in basic aa and is very highly conserved, both within ... | 1997 | 9055825 |
| in vitro effects of an acidic by-product feed on bovine teeth. | to evaluate in vitro erosive effects of sweet potato cannery waste (spcw) on bovine incisor enamel. | 1997 | 9140558 |
| flow injection spectrophotometric determination of l-dopa and carbidopa in pharmaceutical formulations using a crude extract of sweet potato root [ipomoea batatas (l.) lam.] as enzymatic source. | a flow injection (fi) spectrophotometric method is proposed for the determination of l-dopa and carbidopa in pharmaceutical formulations. after selection of the extraction medium (e.g., buffer-to-tissue ratio, ph, buffer concentration, protective agents and/or stabilizers) and storage conditions, crude extract of sweet potato root [ipomoea batatas (l.) lam.] was used as an enzymatic source of polyphenol oxidase (tyrosinase; catechol oxidase; ec.1.14.18.1) directly in the carrier. this enzyme cat ... | 1997 | 9177077 |
| the n-terminal propeptide of the precursor to sporamin acts as a vacuole-targeting signal even at the c terminus of the mature part in tobacco cells. | an asparagine-proline-isoleucine-arginine-leucine (npirl) and its related sequences in the n-terminal propeptides (ntpp) of several plant vacuolar proteins, including that of sporamin from sweet potato (spo) function as vacuole-targeting determinants in a manner that is distinct from the vacuole-targeting determinant in the ctpps of other plant vacuolar proteins. when the mutant precursor to sporamin, spo-ntpp (in which ntpp was moved to the c terminus of the mature part), was expressed in tobac ... | 1997 | 9232873 |
| complete genome structure and phylogenetic analysis of little cherry virus, a mealybug-transmissible closterovirus. | the 5'-terminal genomic region (8597 nt) of little cherry virus (lchv), a mealybug-borne closterovirus, was cloned from double-stranded rna, and its sequence determined to complete the 16934 nt sequence of the monopartite lchv rna genome. in the 5' to 3' direction, the sequence encompasses orf 1a, encoding the conserved replicative domains of methyltransferase and helicase, and orf 1b, encoding rna polymerase. orfs 1a and 1b partially overlap (in o/+1 configuration), and the lchv replicase is pr ... | 1997 | 9267008 |
| expression of a vacuolar protein (vp24) in anthocyanin-producing cells of sweet potato in suspension culture. | vp24, an abundant protein of 24 kd, was found to accumulate in the anthocyanin-containing vacuoles of cells of sweet potato (ipomoea batatas) in suspension culture. light-induced expression of vp24 was analyzed by immunoblotting in three different cell lines that produced anthocyanins at different rates. the expression of vp24 was closely correlated with the accumulation of anthocyanin in these cell lines. immunocytochemical detection of vp24 with specific antibodies on thin sections showed that ... | 1997 | 9390439 |
| [host plants of bemisia tabaci (homoptera: aleyrodidae) in cuba]. | the sweet potato white fly, bemisia tabaci, is an important pest of tomatoes and beans, among other crops, which transmits viral diseases. since the second quarter of 1989 a significant population increase of this pest has been noted in several cultivated plants. from 1989 to 1992, a survey was done throughout the country, chiefly in vegetable and bean-producing areas. they occur in 119 species (42 families), a great increase over the previous record of four species. worldwide, this report repre ... | 1997 | 9404518 |
| proximate composition and selected functional properties of african breadfruit and sweet potato flour blends. | full-fat african breadfruit flour was used to replace 30, 40, 50, 60 and 70% of sweet potato flour. the chemical composition and functional properties of composite flours showed that they contain more protein, fat, and ash and less carbohydrate than sweet potato flour. with increasing level of supplementation of breadfruit, ash, protein and fat contents increased while carbohydrate decreased. the composite flours possessed higher water absorption than sweet potato flour. the water absorption cap ... | 1997 | 9498694 |
| activity staining on polyacrylamide gels of trypsin inhibitors from leaves of sweet potato (ipomoea batatas l. lam) varieties. | the failure of activity staining of trypsin inhibitors in crude leaf extracts of sweet potato varieties including tainong 27, tainong 34, and tainong 57 on a 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) gel was prevented by dipping the gels in solutions containing 10-40 mm hydrogen peroxide, 10 mm tris buffer (ph 7.9) for 30 min before staining. | 1998 | 9548281 |
| effects of gamma irradiation on the flavor composition of food commodities. | fifteen food products including potato, sweet potato, shallot, onion, garlic, ginger, papaya, mango, rice, tobacco, small red bean, mungbean, soybean, wheat, flour and spices have been approved for irradiation by the national health administration in taiwan. market tests (wu et al., 1996) provided strong proof that taiwanese consumers would accept irradiated foods. however, researchers in the food industry are concerned about the possibility of chemical changes, especially in volatile compositio ... | 1998 | 9598207 |
| identification and production of local carotene-rich foods to combat vitamin a malnutrition. | objectives: to address, with respect to improvement of human vitamin a status by dietary approaches, the three theoretical postulates that: 1) the most practical and economical manner to increase the amount of dietary vitamin a available to low-income persons in low-income nations is through plant sources of provitamin a carotenoids; 2) there will be constraints and limitation to the efficiency of a given intervention approach related to behavioural, cultural, biological and botanical considerat ... | 1997 | 9598793 |
| an antigenic protein gene of a phytoplasma associated with sweet potato witches' broom. | a gene encoding the major antigenic protein of phytoplasma associated with sweet potato witches' broom (spwb) was cloned and analysed by screening the genomic library of spwb phytoplasma with monoclonal antibodies for spwb phytoplasma. the entire predicted structural gene encoded an antigenic protein composed of 172 amino acids with a computed molecular mass of 19.15 kda and a pl value of 9.78. the -10 region of the promoter and the terminator region of the gene were identified and found to be s ... | 1998 | 9611800 |
| production of human lactoferrin in transgenic tobacco plants. | production and characterization of human lactoferrin (hlf) in transgenic tobacco is reported. we have engineered two constructs containing either the native signal peptide from human lactoferrin or the signal peptide from sweet potato sporamin fused to human lactoferrin encoding cdna. n-terminal sequences of rhlf purified from tobacco were identical to lf from human milk for both constructs. the tobacco rhlf presents a molecular mass closely identical to native protein. overall sugar composition ... | 1998 | 9631525 |
| complete nucleotide sequence and genome organization of sweet potato feathery mottle virus (s strain) genomic rna: the large coding region of the p1 gene. | the complete nucleotide sequence of a sweet potato feathery mottle virus severe strain (spfmv-s) genomic rna was determined from overlapping cdna clones and by directly sequencing viral rna. the viral rna genome is 10,820 nucleotides long, excluding the poly(a) tail and contains one open reading frame (orf) starting at nucleotide 118 and ending at 10,599, potentially encoding a polyprotein of 3,493 amino acids (mr 393,800). the orf was followed by a 3' untranslated region of 221 nucleotides. the ... | 1997 | 9672618 |
| sequencing and characterization of the coat protein and 3' non-coding region of a new sweet potato potyvirus. | a cdna library was constructed from viral genomic rna purified from sweet potato plants affected by "sweet potato chlorotic dwarf disease" in an attempt to clarify the etiology of this viral complex in argentina. by sequence analysis, some of the obtained clones were found to belong to sweet potato feathery mottle potyvirus (spfmv), to a closterovirus and to a new potyvirus. a cdna clone of 1,103 bp representing the coat protein cistron and 3' non-coding region of the newly identified potyvirus ... | 1997 | 9672624 |
| biochemical and spectroscopic characterization of catechol oxidase from sweet potatoes (ipomoea batatas) containing a type-3 dicopper center. | two catechol oxidases have been isolated from sweet potatoes (ipomoea batatas) and purified to homogeneity. the two isozymes have been characterized by exafs, epr-, uv/vis-spectroscopy, isoelectric focusing, and maldi-ms and have been shown to contain a dinuclear copper center. both are monomers with a molecular mass of 39 kda and 40 kda, respectively. substrate specificity and nh2-terminal sequences have been determined. exafs data for the 39 kda enzyme reveal a coordination number of four for ... | 1998 | 9781698 |
| rna extraction from virus-diseased sweet potato for reverse transcriptase polymerase chain reaction analysis. | sweet potato virus disease (spvd), which causes severe crop losses in africa, is caused by a complex of sweet potato feathery mottle potyvirus (spfmv) and sweet potato chlorotic stunt crinivirus (spcsv). extraction of pure rna (for diagnosis by reverse transcriptase polymerase chain reaction [rt-pcr] methods) from diseased sweet potato proved to be difficult using five different total-rna extraction procedures: (a) that of chomczynski and sacchi (1) (b) an adaptation of lodhi et al., (2) and thr ... | 1998 | 9819817 |
| dried green leaf powders of jew's mellow (corchorus), persimmon (diosphyros kaki) and sweet potato (ipomoea batatas poir) lower hepatic cholesterol concentration and increase fecal bile acid excretion in rats fed a cholesterol-free diet. | a cholesterol-free diet containing dried powder of jew's mellow leaves, persimmon leaves or sweet potato leaves respectively at 5% level as dietary fiber was fed to male sprague-dawley rats for about one month. the experiment was conducted twice except for sweet potato leaves. in the groups fed the diet mixed with powders of any of the three different dried green leaves, the hepatic cholesterol concentration significantly decreased. such lowering was not observed in serum cholesterol concentrati ... | 1998 | 9839835 |
| sequence analysis of the coat protein and 3'-noncoding region for korean and other strains of sweet potato feathery mottle virus. | the 3'-terminal regions of the genomic rnas of two korean isolates of sweet potato feathery mottle potyvirus (spfmv) were cloned and their nucleotide sequences of full-length coat protein (cp) gene and 3' noncoding region (ncr) were determined. the cp of the two korean isolates contained 315 amino acid residues. the cp cistron sequences of the korean isolates exhibit 72.7% to 98.7% nucleotide sequence identity and 79.9% to 99.0% amino acid identity when compared with those of 8 other known spfmv ... | 1998 | 9895134 |
| l-galactono-gamma-lactone dehydrogenase from sweet potato: purification and cdna sequence analysis. | l-galactono-gamma-lactone dehydrogenase (ec 1.3.2.3, gldhase) was partially purified from mitochondria of sweet potato tuberous roots over 600-fold on a specific activity basis, followed by purification of the enzyme protein of 56 kda by a preparative sds-page. the absorption spectrum of the hydroxylapatite column-purified gldh-ase showed peaks at 448 and 373 nm, suggesting the presence of flavin as a prosthetic group. the activity of gldh-ase was inhibited by lycorine, an alkaloid which inhibit ... | 1998 | 10050319 |
| the active site of purple acid phosphatase from sweet potatoes (ipomoea batatas) metal content and spectroscopic characterization. | purple acid phosphatase from sweet potatoes ipomoea batatas (sppap) has been purified to homogeneity and characterized using spectroscopic investigations. matrix-assisted laser desorption/ionization mass spectrometry analysis revealed a molecular mass of approximately 112 kda. the metal content was determined by x-ray fluorescence using synchrotron radiation. in contrast to previous studies it is shown that sppap contains a fe(iii)-zn(ii) center in the active site as previously determined for th ... | 1999 | 10102999 |
| entomopathogenic potential of verticillium and acremonium species (deuteromycotina: hyphomycetes). | hyphomycetes with conidia formed in slimy heads from hyaline mycelium were isolated from a range of insect hosts. isolation on artificial medium and microscopic examination revealed that these fungi in many cases were not verticillium lecanii despite a superficial resemblance to this common entomopathogen. the fungi were identified as verticillium fusisporum, verticillium psalliotae, verticillium lamellicola, and species of acremonium. isolates of these fungi were bioassayed against the sweet-po ... | 1999 | 10222186 |
| antimutagenicity of sweetpotato (ipomoea batatas) roots. | antimutagenicity of the water extracts prepared from the storage roots of four varieties of sweetpotato with different flesh colors was investigated using salmonella typhimurium ta 98. the extract from the whole roots of the purple-colored ayamurasaki variety effectively decreased the reverse mutation induced not only by trp-p-1, trp-p-2, iq, b[a]p, and 4-nqo but also by dimethyl sulfoxide extracts of grilled beef. comparison of the inhibitory activity of the extracts from the normal ayamurasaki ... | 1999 | 10227139 |
| isolation of the gene encoding an immunodominant membrane protein of the apple proliferation phytoplasma, and expression and characterization of the gene product. | an immunodominant membrane protein (imp) of the apple proliferation (ap) phytoplasma was detected in preparations from ap-diseased periwinkle plants using monoclonal and polyclonal antibodies to the ap agent. following isolation from western blots and partial sequencing, degenerate oligonucleotides derived from the imp sequence were used as probes to identify a dna fragment containing the orf encoding the imp. complete sequencing and subsequent analysis of the cloned dna fragment revealed the pr ... | 1999 | 10463160 |
| molecular characterization of cdnas for two anionic peroxidases from suspension cultures of sweet potato. | two cdnas for anionic peroxidase (pods), swpa2 and swpa3, were isolated from suspension cultures of sweet potato (ipomoea batatas), and their expression was investigated with a view to understanding the physiological function of pods in relation to environmental stresses. swpa2 (whose putative mature protein product would have a pi value of 4.1) and swpa3 (4.3) encode polypeptides of 358 and 349 amino acids, respectively. the genes from which they were derived are predominantly expressed in cult ... | 1999 | 10485284 |
| dioscorin, the major tuber storage protein of yam (dioscorea batatas decne) with carbonic anhydrase and trypsin inhibitor activities. | dioscorin, the tuber storage protein of yam (dioscorea batatas decne), was purified successively by ammonium sulfate fractionation, de-52 ion exchange chromatography, and sephadex g-75 column. two protein bands (82 and 28 kda) were found under nonreducing conditions after sds-page; but only one band (32 kda) was detected under reducing conditions. the first 21 amino acids in the n-terminal region of the 28 kda form were vedefsyiegnpngpenwgnl, which was highly homologous to deductive sequence of ... | 1999 | 10552514 |
| expression patterns of two genes for the delta-subunit of mitochondrial f1-atp synthase from sweet potato in transgenic tobacco plants and cells. | two nuclear genes, f1 delta-1 and f1 delta-2, coding for the delta-subunit of mitochondrial f1-atp synthase, which corresponds to oligomycin-sensitivity conferring protein in animal and yeast mitochondria, were isolated from sweet potato. the gene for the delta-subunit was composed of 6 exons and these two genes shared high sequence similarities to each other not only in exons but also in introns and in the 5'-upstream regions. however, the 5'-upstream regions of f1 delta-1 and f1 delta-2 were d ... | 1999 | 10555307 |
| cloning and comparative protein modeling of two purple acid phosphatase isozymes from sweet potatoes (ipomoea batatas). | the sequence of cdna fragments of two isozymes of the purple acid phosphatase from sweet potato (sppap1 and sppap2) has been determined by 5' and 3' rapid amplification of cdna ends protocols using oligonucleotide primers based on amino acid information. the encoded amino acid sequences of these two isozymes show an equidistance of 72-77% not only to each other, but also to the primary structure of the purple acid phosphatase from red kidney bean (kbpap). a three-dimensional model of the active ... | 1999 | 10556574 |
| post-translational maturation of natural and drug-induced missorted phytohemagglutinin. | the bean lectin phytohemagglutinin (pha) was expressed in transgenic suspension-cultured by-2 tobacco cells simultaneously with another recombinant vacuolar protein, the sweet potato sporamin. in contrast to previous observations in different transgenic plant systems when expressed in by-2 tobacco cells, phytohemagglutinin is mostly but not exclusively targeted to the vacuole. indeed, a small amount of recombinant phytohemagglutinin is secreted into the culture medium of tobacco cells. furthermo ... | 1999 | 10580285 |
| performance of broiler chickens fed sweet potato meal (ipomoea batatas l.) diets. | day-old lohman broiler chicks (n = 120) were fed on five starting diets for 4 weeks in groups of 24 birds. the starting diets contained 0%, 9%, 18%, 27% and 36% sweet potato tuber as a replacement for maize. from the fifth week, the 120 birds were tested in groups of 30 on four finishing diets containing 0%, 15%, 30% and 45% sweet potato tuber as a replacement for maize. the carcass quality was significantly (p < 0.05) improved due to a significant (p < 0.05) reduction of abdominal fat in the bi ... | 1999 | 10599133 |
| the n-terminal propeptide and the c terminus of the precursor to 20-kilo-dalton potato tuber protein can function as different types of vacuolar sorting signals. | two types of vacuolar sorting signals (vsss), an asparagine-proline-isoleucine-arginine-leucine (npirl)-related vss in the n-terminal propeptides (ntpps) and a c-terminal vss in the c-terminal propeptides (ctpps), function differently in plant cells. a precursor to a 20-kda protein of potato tuber (pt20) contains two npirl-related sequences, npinl in a short ntpp and npldv close to the c terminus of the precursor. we made mutant forms of sweet potato sporamin (spo), npt20-spo, in which the n-ter ... | 1999 | 10635117 |
| purification and characterization of chitin-binding proteins from the hemolymph of sweet potato hornworm, agrius convolvuli. | three chitin-binding proteins (cbps: cbp9, cbp15, cbp66) were identified from the larval hemolymph of sweet potato hornworm, agrius convolvuli. two (cbp9 and cbp15) of them have been isolated and purified by gel filtration (superdex hr 75), cation-exchange chromatography (mono s), and reverse-phase chromatography (murpc pc 2.1/3). in experiments to detect cbps in hemolymph, we examined whether ionic strength and existence of bovine serum albumin in the incubation solution influenced binding affi ... | 1999 | 10665375 |
| antidiabetic activity of white skinned sweet potato (ipomoea batatas l.) in obese zucker fatty rats. | antidiabetic effects of white skinned sweet potato (ipomoea batatas l.) (wssp) and troglitazone, an insulin sensitizer, were investigated. hyperinsulinemia in zucker fatty rats was reduced by 23%, 26%, 60% and 50%, respectively, 3, 4, 6 and 8 weeks after starting the oral administration of wssp. similar results were obtained with troglitazone. in the glucose tolerance test after 7 weeks of treatment, increases in blood glucose levels after glucose loading were inhibited by the administration of ... | 2000 | 10706405 |
| nutritional quality and the presence of anti-nutritional factors in leaf protein concentrates (lpc). | leaf protein concentrates were prepared from 25 different plant species. the concentrates contained acceptable levels of all essential amino acids; except methionine which was limiting with chemical scores ranging between 13.5-96%. lpcs from sesbania, nebergrass, lufa and sweet pepper were lethal to the rats. per ranged between 0.28 for safflower lpc up to 1.95 for rape. positive nitrogen balance was obtained with diets based on lpcs with mean values ranging between 6.4 and 22.42 mg n per day. t ... | 1999 | 10719564 |
| synergistic interactions of a potyvirus and a phloem-limited crinivirus in sweet potato plants. | when infecting alone, sweet potato feathery mottle virus (spfmv, genus potyvirus) and sweet potato chlorotic stunt virus (spcsv, genus crinivirus) cause no or only mild symptoms (slight stunting and purpling), respectively, in the sweet potato (ipomoea batatas l. ). in the spfmv-resistant cv. tanzania, spfmv is also present at extremely low titers, though plants are systemically infected. however, infection with both viruses results in the development of sweet potato virus disease (spvd) charact ... | 2000 | 10725195 |
| chromosome mapping of the sweet potato little leaf phytoplasma reveals genome heterogeneity within the phytoplasmas. | to further understand the genomic diversity and genetic architecture of phytoplasmas, a physical and genetic map of the sweet potato little leaf (spll) strain v4 phytoplasma chromosome was determined. pfge was used to determine the size of the spll-v4 genome, which was estimated to be 622 kb. a physical map was prepared by two-dimensional reciprocal digestions using the restriction endonucleases bsshii, smal, eagl and i-ceul. sixteen cleavage sites were located on the map. southern hybridization ... | 2000 | 10784048 |
| comparisons of coat protein gene sequences show that east african isolates of sweet potato feathery mottle virus form a genetically distinct group. | sweet potato feathery mottle virus (spfmv, genus potyvirus) infects sweet potatoes (ipomoea batatas) worldwide, but no sequence data on isolates from africa are available. coat protein (cp) gene sequences from eight east african isolates from madagascar and different districts of uganda (the second biggest sweet potato producer in the world) and two west african isolates from nigeria and niger were determined. they were compared by phylogenetic analysis with the previously reported sequences of ... | 2000 | 10795523 |
| sublethal effects of resin glycosides from the periderm of sweetpotato storage roots on plutella xylostella (lepidoptera: plutellidae). | resin glycoside material extracted from the periderm tissue of storage roots from sweetpotato, ipomoea batatas (l.) lam., was bioassayed for effects on survival, development, and fecundity of the diamondback moth, plutella xylostella (l.). the resin glycoside was incorporated into an artificial diet and fed to p. xylostella larvae. first instars were placed individually into snap-top centrifuge vials containing artificial diet with one of six concentrations of resin glycoside material (0.00, 0.2 ... | 2000 | 10826190 |
| differential expression of four sweet potato peroxidase genes in response to abscisic acid and ethephon. | expression of four peroxidase (pod) genes, three anionic pods (swpa1, swpa2 and swpa3), and one neutral pod (swpn1) isolated from suspension cultures of sweet potato (ipomoea batatas) were analyzed by measuring the accumulation of transcripts in suspension cultured cells and leaves of sweet potato in response to the stress-related plant hormones abscisic acid (aba) and ethephon (an ethylene generating chemical). the four genes responded differently to aba (0.1 mm) and ethephon (0.1 mm) in cultur ... | 2000 | 10846741 |
| identification of mammalian-like purple acid phosphatases in a wide range of plants. | purple acid phosphatases (paps) comprise a family of binuclear metal-containing hydrolases, members of which have been isolated from plants, mammals and fungi. polypeptide chains differ in size (animal approximately 35kda, plant approximately 55kda) and exhibit low sequence homology between kingdoms but all residues involved in co-ordination of the metal ions are invariant. a search of genomic databases was undertaken using a sequence pattern which includes the conserved residues. several novel ... | 2000 | 10854785 |
| the plant vacuolar sorting receptor atelp is involved in transport of nh(2)-terminal propeptide-containing vacuolar proteins in arabidopsis thaliana. | many soluble plant vacuolar proteins are sorted away from secreted proteins into small vesicles at the trans-golgi network by transmembrane cargo receptors. cleavable vacuolar sorting signals include the nh(2)-terminal propeptide (ntpp) present in sweet potato sporamin (spo) and the cooh-terminal propeptide (ctpp) present in barley lectin (bl). these two proteins have been found to be transported by different mechanisms to the vacuole. we examined the ability of the vacuolar cargo receptor atelp ... | 2000 | 10871276 |
| [assessment of the impact of the biological rodenticide biorat in populations of rodents settled in several crops of the republic of costa rica]. | the impact of biological rodenticide biorat on populations of rodents settled in some crops was determined; these crops are highly infested with sigmodon hispidus, a very common vector in various central american countries. the trapping technique and the appraisal of damages allowed to calculate the index of infestation which ranged from 75 to 90 percent in three crops. twenty for to 36 kg of biorat were applied to 6 ha planted with cucumis sativus (cucumber), ipomoea batata (sweet potato) and a ... | 1999 | 10887586 |
| effects of extrusion cooking on functional properties of mixtures of full-fat soy and sweet potato. | full-fat soy and sweet potato were extruded at 0:100, 25:75, 50:50, 75:25 and 100:0 ratios in an insta-pro 600 single screw extruder operated at 180, 200, 220 rpm respectively. functional properties: bulk density, expansion ratio, water absorption and solubility indices and trypsin inhibitor were assayed. results showed that addition of soybeans to sweet potatoes considerably increased the protein, fat, ash and trypsin inhibitor levels of the raw material mixtures. it also led to decreases in vi ... | 1998 | 10890756 |
| delayed activation of post-transcriptional gene silencing and de novo transgene methylation in plants with the coat protein gene of sweet potato feathery mottle potyvirus. | the relationship between post-transcriptional gene silencing (ptgs) and dna methylation was examined using nicotiana benthamiana transformed with the coat protein gene including the 3' non-translated region of sweet potato feathery mottle potyvirus. line 4.28 showed a delayed activation of the transgene silencing in comparison with the other silenced lines, and showed complete resistance against the recombinant potato virus x engineered to contain the sequence homologous to the transgene when th ... | 2000 | 10936519 |
| a complex containing both trypsin inhibitor and dehydroascorbate reductase activities isolated from mitochondria of etiolated mung bean (vigna radiata l. (wilczek) cv. tainan no. 5) seedlings. | a complex containing trypsin inhibitor (ti) activity was extracted with 0.1 m tris buffer (ph 7.9) from trypsin-treated mitochondria of etiolated mung bean seedlings, and further purified with a superdex 200 fplc column. this partially purified complex with an m(r) about 820 kda exhibited additional dehydroascorbate (dha) reductase activity with specific activities of 0.21, 1.53 and 1.54 mumol ascorbate formed min-1 mg-1 protein at ph 6.0, 6.5 and 7.0, respectively, when glutathione was added. m ... | 2000 | 10938863 |
| inoculation of somatic embryos of sweet potato with an arbuscular mycorrhizal fungus improves embryo survival and plantlet formation. | responses of somatic embryos of sweet potato (ipomoea batata (l.) poir., cv. white star) at different developmental stages to in vitro inoculation with glomus etunicatum (becker and gerdemann) (isolate invam fl329) were evaluated. somatic embryos were grown in glass tubes containing sterilized vermiculite and sand. a layer of natrosol plus white's medium was used as a carrier for arbuscular mycorrhizal (am) fungal spores. survival of embryos inoculated with am fungi was significantly (p < 0.05) ... | 2000 | 10941521 |
| molecular characterization of tobacco mitochondrial l-galactono-gamma-lactone dehydrogenase and its expression in escherichia coli. | a cdna clone encoding l-galactono-gamma-lactone (gal) dehydrogenase (ec 1.3.2.3) was isolated from tobacco leaves. the cdna clone contained an open reading frame encoding the protein of 501 amino acids with a calculated molecular mass of 56,926 da, preceded by a putative mitochondrial targeting signal consisting of 86 amino acid residues. in fact, gal dehydrogenase was localized in the mitochondria of tobacco cells. the deduced amino acid sequence of the cdna showed 77 and 82% homology to caulif ... | 2000 | 10945335 |
| cytological and molecular evidence that the whitefly-transmitted cucumber vein yellowing virus is a tentative member of the family potyviridae. | cucumber vein yellowing virus (cvyv) is widespread in cucurbits in the middle east. cvyv has filamentous particles and is transmitted by bemisia tabaci by the semi-persistent mode. it has not yet been assigned to a specific genus or family. ultramicroscopic observations revealed numerous cylindrical cytoplasmic inclusions in melon and cucumber cells infected by cvyv isolates from israel and jordan. depending on the section orientation, the inclusions appeared as pinwheels or as bundles. in addit ... | 2000 | 10950987 |
| monosaccharide composition of sweetpotato fiber and cell wall polysaccharides from sweetpotato, cassava, and potato analyzed by the high-performance anion exchange chromatography with pulsed amperometric detection method. | the cell wall materials (cwms) from sweetpotato (ipomoea batatas cv. kokei 14), cassava (manihot esculenta), and potato (solanum tuberosum cv. danshaku) and commercial sweetpotato fiber as well as their polysaccharide fractions were analyzed for sugar composition by the high-performance anion exchange chromatography with pulsed amperometric detection (hpaec-pad) method. the separation of arabinose and rhamnose, and xylose and mannose, by this method has been improved using a carbopac pa 10 colum ... | 2000 | 10956132 |
| latin american food sources of carotenoids. | latin america has a wide variety of carotenogenic foods, notable for the diversity and high levels of carotenoids. a part of this natural wealth has been analyzed. carrot, red palm oil and some cultivars of squash and pumpkin are sources of both beta-carotene and alpha-carotene. beta-carotene is the principal carotenoid of the palm fruits burití, tucumã and bocaiuva, other fruits such as loquat, marolo and west indian cherry, and sweet potato. buriti also has high amounts of alpha-carotene and g ... | 1999 | 10971848 |
| anthocyanins in callus induced from purple storage root of ipomoea batatas l. | two anthocyanins were isolated from the highly pigmented callus derived from the storage root of purple sweet potato (ipomoea batatas l.) cultivar 'ayamurasaki'. one was identified as cyanidin 3-o-sophoroside-5-o-glucoside, and the other as cyanidin 3-o-(2-o-(6-o-(e)-p-coumaroyl-beta-d-glucopyranosyl)-beta-d-glucop yranoside)-5-o-beta-d-glucopyranoside, by chemical and spectroscopic analysis. | 2000 | 11014289 |
| chemical evaluation of multimixes formulated from some local staples for use as complementary foods in nigeria. | the nutrient compositions of eight multimixes formulated for use as complementary foods from processed soybeans, cowpeas, maize, sorghum, yams, cocoyams, plantains and sweet potatoes were examined. the foods were processed by sprouting, cooking and fermentation. the samples were separately dried and milled to fine flours. a ratio of 65% cereal, 30% legume and 5% starchy staple (65:30:5) calculated on a protein basis was used to formulate the multimixes. the blends were chemically analyzed using ... | 2000 | 11030479 |
| new skeletal sesquiterpenoids, caprariolides a-d, from capraria biflora and their insecticidal activity. | four structurally novel isomeric sesquiterpenes have been isolated from the aerial parts of capraria biflora. caprariolides a (1), b (2), c (3), and d (4) have been determined by nmr spectroscopy experiments to be the (3s,5s,9r), (3r,5s,9r), (3r,5r,9r), and (3s,5r, 9r) isomers of 7-(furan-3'-yl)-3,9-dimethyl-1-oxaspiro[4. 5]dec-6-en-2-one, respectively. both 1 and 2 were found to exhibit insecticidal activity against adult cylas formicarius elegantulus, one of the most destructive insect pests o ... | 2000 | 11087595 |
| invertase proteinaceous inhibitor of cyphomandra betacea sendt fruits. | this work describes a new invertase proteinaceous inhibitor from cyphomandra betacea sendt. (tomate de arbol) fruits. the proteinaceous inhibitor was isolated and purified from a cell wall preparation. the ph stability, kinetics of the inhibition of the c. betacea invertase, inhibition of several higher plant invertases and lectin nature of the inhibitor were studied. the inhibitor structure involves a single polypeptide (mr = 19000), as shown by gel filtration and sds-page determinations. n-ter ... | 2000 | 11140613 |
| osmotic stress-induced changes of sucrose metabolism in cultured sweet potato cells. | the intra- and extracellular sugar contents, the activities of sucrose-metabolizing enzymes, and the metabolism of [u-(14)c] glucose in a pulse-chase experiment were compared between the normal and osmotically stressed (by 0.6 m sorbitol) sweet potato (ipomoea batatas) suspension cells. the stress enhanced the levels of sucrose and sucrose phosphate synthase (sps) activity. northern blot analysis also showed that prolonged osmotic stress enhanced the sps gene expression at the transcriptional le ... | 2000 | 11141173 |
| identification of the maize amyloplast stromal 112-kd protein as a plastidic starch phosphorylase. | amyloplast is the site of starch synthesis in the storage tissue of maize (zea mays). the amyloplast stroma contains an enriched group of proteins when compared with the whole endosperm. proteins with molecular masses of 76 and 85 kd have been identified as starch synthase i and starch branching enzyme iib, respectively. a 112-kd protein was isolated from the stromal fraction by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subjected to tryptic digestion and amino acid sequence a ... | 2001 | 11154342 |
| primary structure and expression of a 24-kd vacuolar protein (vp24) precursor in anthocyanin-producing cells of sweet potato in suspension culture. | a 24-kd vacuolar protein (vp24) accumulates abundantly in intravacuolar pigmented globules in anthocyanin-containing sweet potato (ipomoea batatas) cells in suspension culture. a cdna clone encoding vp24 was isolated from a cdna library constructed from light-irradiated suspension-cultured cells. sequence analysis revealed that a 2.9-kbp vp24 cdna encodes a protein of 893 amino acid residues with a molecular mass of 96.3 kd. according to the deduced amino acid sequence of vp24 cdna, vp24 is prob ... | 2001 | 11154352 |
| detection and characterization of a 36-kda peptide in c-terminal region of a 24-kda vacuolar protein (vp24) precursor in anthocyanin-producing sweet potato cells in suspension culture. | a 24-kda vacuolar protein (vp24) was found to accumulate in anthocyanin-producing sweet potato cells (ipomoea batatas lam.) in suspension culture [nozue et al., plant physiol. 115 (1997) 1065]. vp24 cdna (accession no. ab025531) encodes a 96.3-kda large precursor protein with a c-terminal propeptide which contains the eight putative transmembrane domains. the mature vp24 is probably involved in the formation of intravacuolar pigmented globules (cyanoplasts) in highly anthocyanin-containing vacuo ... | 2000 | 11164584 |
| construction and analytical application of a biosensor based on stearic acid-graphite powder modified with sweet potato tissue in organic solvents. | a biosensor based on stearic acid-graphite powder modified with sweet potato (ipomoea batatas (l.) lam.) tissue as peroxidase source was constructed and applied in organic solvents. several parameters were studied to evaluate the performance of this biosensor such as stearic acid-graphite powder and tissue composition, type and concentration of supporting electrolyte, organic solvents, water/organic solvent ratio (% v/v) and hydrogen peroxide concentration. after selection of the best conditions ... | 2000 | 11227501 |
| study on tablet binding and disintegrating properties of alternative starches prepared from taro and sweet potato tubers. | to demonstrate the potential alternative sources of starch used in tablet formulations, starches from taro (ts) and sweet potato (sps) tubers were prepared with obtained yields of 11.0 and 9.6%, respectively. both ts and sps met usp22-nf17 identification and specifications. their equilibrium moisture contents and gelatinization temperatures were comparable with those of commercial starch, whereas amylose contents of ts and sps were 21.38% w/w and 41.76% w/w, respectively. both were found to poss ... | 2001 | 11247539 |
| isolation of antidiabetic components from white-skinned sweet potato (ipomoea batatas l.). | we have already reported that white-skinned sweet potato (ipomoea batatas l.) (wssp) shows antidiabetic activity in streptozotocin (stz) induced diabetic rats and genetically diabetic models (yellow kk, db/db mice and zucker fatty rats). in this study, isolation and purification of the antidiabetic component of wssp were attempted. almost all antidiabetic activity was found in the cortex of wssp. the fractionation of the antidiabetic component in the wssp cortex was done by the following methods ... | 2001 | 11272813 |
| phase ii study of 4-ipomeanol, a naturally occurring alkylating furan, in patients with advanced hepatocellular carcinoma. | 4-ipomeanol (ipo; nsc 394438), a naturally occurring furan isolated from common sweet potatoes (ipomoea batatas) infected with the fungus fusarium solani was the first agent to be developed by the national cancer institute based on a biochemical-biological rationale as an anticancer agent targeted specifically against lung cancer. prior to clinical development, ipo was shown to induce pulmonary toxicity in the lungs of several mammalian species because the agent is metabolized to a highly reacti ... | 2001 | 11291834 |
| alpha-glucosidase inhibitory action of natural acylated anthocyanins. 1. survey of natural pigments with potent inhibitory activity. | alpha-glucosidase (agh) inhibitory study by natural anthocyanin extracts was done. as the result of a free agh assay system, 12 anthocyanin extracts were found to have a potent agh inhibitory activity; in particular, pharbitis nil (soa) extract showed the strongest maltase inhibitory activity, with an ic(50) value of 0.35 mg/ml, as great as that of ipomoea batatas (ygm) extract (ic(50) = 0.36 mg/ml). interestingly, neither extract inhibited the sucrase activity at all. for the immobilized assay ... | 2001 | 11308351 |
| alpha-glucosidase inhibitory action of natural acylated anthocyanins. 2. alpha-glucosidase inhibition by isolated acylated anthocyanins. | four diacylated pelargonidin (pg: soa-4 and soa-6), cyanidin (cy: ygm-3), and peonidin (pn: ygm-6) 3-sophoroside-5-glucosides isolated from the red flowers of the morning glory, pharbitis nil cv. scarlett o'hara (soa), and the storage roots of purple sweet potato, ipomoea batatas cv. ayamurasaki (ygm), were subjected to an alpha-glucosidase (agh) inhibitory assay, in which the assay was performed with the immobilized agh (iagh) system to mimic the membrane-bound agh at the small intestine. as a ... | 2001 | 11308352 |
| biotransformation of squamulosone by curvularia lunata atcc 12017. | squamulosone (aromadendr-1(10)-en-9-one), isolated in large quantity from the plant hyptis verticillata jacq. (labiatae), was incubated with the fungus curvularia lunata atcc 12017 in two different growth media. six metabolites were isolated from each medium. with five of the products being common to both fermentations. all seven metabolites are novel. the insecticidal activity of these aromadendranes was evaluated against the sweet potato weevil cylas formicarius elegantus. | 2001 | 11393517 |
| antioxidant activities of trypsin inhibitor, a 33 kda root storage protein of sweet potato (ipomoea batatas (l.) lam cv. tainong 57). | trypsin inhibitors (tis), root storage proteins, were purified from sweet potato (ipomoea batatas[l.] lam cv. tainong 57) roots by trypsin affinity column according to the methods of hou and lin (plant sci. 1997, 126, 11-19 and plant sci. 1997, 128, 151-158). a single band of 33 kda ti was obtained by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) gels. this purified 33 kda ti had scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (dpph) radical. there wa ... | 2001 | 11409996 |
| alpha-tocopherol content in 62 edible tropical plants. | vitamin e was determined by the high-performance liquid chromatography (hplc) method. all the plants tested showed differences in their alpha-tocopherol content and the differences were significant (p < 0.05). the highest alpha-tocopherol content was in sauropus androgynus leaves (426.8 mg/kg edible portion), followed by citrus hystrix leaves (398.3 mg/kg), calamus scipronum (193.8 mg/kg), starfruit leaves averrhoa belimbi (168.3 mg/kg), red pepper capsicum annum (155.4 mg/kg), local celery apiu ... | 2001 | 11410015 |
| biosensors based on novel peroxidases with improved properties in direct and mediated electron transfer. | native horseradish peroxidase (hrp) on graphite has revealed approximately 50% of the active enzyme molecules to be in direct electron transfer (et) contact with the electrode surface. some novel plant peroxidases from tobacco, peanut and sweet potato were kinetically characterised on graphite in order to find promising candidates for biosensor applications and to understand the nature of the direct et in the case of plant peroxidases. from measurements of the mediated and mediatorless currents ... | 2000 | 11419644 |
| chemical, sensory and rheological properties of porridges from processed sorghum (sorghum bicolor), bambara groundnut (vigna subterranea l. verdc) and sweet potato (ipomoea batatas) flours. | the chemical, sensory and rheological properties of porridges made from blends of sprouted sorghum, bambara groundnuts and fermented sweet potatoes were examined. sorghum and bambara groundnuts were sprouted for 48 h while sweet potatoes were fermented for the same period. blends were formulated from the processed ingredients in the ratio of 60:40:0, 57:42:1, 55:44:1 and 52:46:2 (protein basis) of sorghum, bambara groundnuts and sweet potatoes. porridges were prepared from the composite flours a ... | 2001 | 11442225 |
| isozymes of ipomoea batatas catechol oxidase differ in catalase-like activity. | the amino acid sequences of two isozymes of catechol oxidase from sweet potatoes (ipomoea batatas) were determined by edman degradation of brcn cleavage fragments of the native protein and by sequencing of amplified cdna fragments. sequence alignment and phylogenetic analysis of plant catechol oxidases revealed about 80% equidistance between the two i. batatas catechol oxidases and approximately 40--60% to catechol oxidases of other plants. when h(2)o(2) was applied as substrate the 39 kda isozy ... | 2001 | 11451442 |
| bovine atypical interstitial pneumonia associated with the ingestion of damaged sweet potatoes (ipomoea batatas) in northeastern brazil. | atypical interstitial pneumonia in cattle associated with the ingestion of damaged sweet potatoes is reported in the state of paraíba, northeastern brazil. the sweet potatoes were severely damaged by myzus tersicae, and had an obvious fungal infection. eighteen milking cows, 1 bull and 1 steer were fed approximately 400 kg of the sweet potatoes. six days after consumption 13 cows were affected with labored abdominal breathing, extended and lowered head, coughing, expiratory grunt, salivation and ... | 2001 | 11474732 |
| morphohistobiochemical characteristics of embryogenic and nonembryogenic callus cultures of sweet potato (ipomoea batatas l.). | ipomoea batatas callus culture raised in a medium supplemented with 2,4-d (2,4-dichlorophenoxy acetic acid) alone or 2,4-d in combination with benzyl adenine, were found to be embryogenic. supplementation of exogenous chemicals, such as 5 g/l naci or 0.7 g/l proline together with a mild dose of 0.2 mg/l 2,4-d, enhanced somatic embryogenesis significantly in all the genotypes tested. morphological, growth, physiological, histological, and biochemical characteristics of the embryogenic callus were ... | 2001 | 11503972 |
| sugar-responsible elements in the promoter of a gene for beta-amylase of sweet potato. | expression of genes coding for sporamin and beta-amylase, the two most abundant proteins in storage roots of sweet potato, is coordinately inducible in atypical vegetative tissues by sugars. a sweet potato gene for beta-amylase (beta-amy) with introns as well as a beta-amy::gus fusion gene composed of the beta-amy promoter and the gus coding sequence, both showed sugar-inducible expression in leaves of transgenic tobacco which occurred via a hexokinase-independent pathway. analyses using various ... | 2001 | 11516155 |
| food poisoning due to methamidophos-contaminated vegetables. | organophosphate poisoning is well known for its characteristic symptoms and signs, but food poisoning caused by pesticide-contaminated food is seldom reported. | 2001 | 11527224 |
| growing root, tuber and nut crops hydroponically for celss. | among the crops selected by the national aeronautics and space administration for growth in controlled ecological life support systems are four that have subsurface edible parts -- potatoes, sweet potatoes, sugar beets and peanuts. these crops have been produced in open and closed (recirculating), solid media and liquid, hydroponic systems. fluorescent , fluorescent plus incandescent and high pressure sodium plus metal halide lamps have proven to be effective light sources. continuous light with ... | 1992 | 11537058 |
| food production and nutrition in biosphere 2: results from the first mission september 1991 to september 1993. | the initial test of the biosphere 2 agricultural system was to provide a nutritionally adequate diet for eight crew members during a two year closure experiment, 1991-1993. the overall results of that trial are presented in this paper. the 2000 m2 cropping area provided about 80 percent of overall nutritional needs during the two years. adaptation of the crew to the diet which averaged 2200 calories, 73 g. of protein and 32 g. of fat per person over the course of the two years. the diet was ... | 1996 | 11538814 |
| analysis of plant harvest indices for bioregenerative life support systems. | harvest indices, which are measures of the ratio of edible to total plant weight, are redefined to include edible sugars derived from enzymatic hydrolysis of the cellulose content of inedible plant components. compositional analysis and carbohydrate contents of rapeseed, rice, soybeans, cowpea, wheat, sweet potato, white potato, and lettuce were analyzed to develop such generalized harvest indices. cellulose conversion is shown to extend considerably the food available from plants otherwise grow ... | 1995 | 11541096 |
| zinc bioavailability in young adult rats fed sweet potato greens containing phytic acid. | this study determined whether phytic acid in sweet potato greens influences the bioavailability of zinc in young adult rats used as models for adult humans. a control diet (ain-93m), two ain-93m diets with pure phytic acid (pa) at 0.2% or 0.4%, and four diets with georgia jet or tu-82-155 dried blanched greens at 7.5% or 15% were fed to seven groups of 7-week male harlan-sprague rats for 21 days. weight gains were generally not affected by pa concentration, were lower in the rats fed with sweet ... | 1999 | 11542237 |
| food production and nutrition for the crew during the first 2-year closure of biosphere 2. | biosphere 2's finite natural resources: atmosphere, plants, water, and soil, and its unique increased rate of nutrient cycling, mandated a design for the agriculture that emphasized sustainability and high productivity. the results of the initial 2-year test of the agriculture system showed that it could provide a diet that was both nutritionally adequate and pleasing to the palate of the eight-member crew from september 1991 to september 1993. the agriculture design was developed from 1985 to 1 ... | 1997 | 11542292 |
| integrating biological treatment of crop residue into a hydroponic sweetpotato culture. | residual biomass from hydroponic culture of sweetpotato [ipomoea batatas (l.) lam.] was degraded using natural bacterial soil isolates. sweetpotato was grown for 120 days in hydroponic culture with a nutrient solution comprised of a ratio of 80% modified half hoagland solution to 20% filtered effluent from an aerobic starch hydrolysis bioreactor. the phytotoxicity of the effluent was assayed with waldmann's green' lettuce (lactuca sativa l.) and the ratio selected after a 60-day bioassay using s ... | 1997 | 11542554 |
| nitrogen recycling during phenylpropanoid metabolism in sweet potato tubers. | in the first step of the phenylpropanoid metabolic pathway, l-phenylalanine (l-phe) is deaminated to form e-cinnamate, in a conversion catalyzed by phenylalanine ammonia-lyase (pal; ec 4.3.1.5). the metabolic fate of the ammonium ion (nh4+) produced in this reaction was investigated in sweet potato (ipomoea batatas) tuber discs. [15n]-labeled substrates including l-phe, in the presence or absence of specific enzyme inhibitors, were administered to sweet potato discs in light under aseptic condit ... | 1998 | 11543585 |
| effect of nitrogen on resistance of sweet potato to sweetpotato weevil (coleoptera: curculionidae) and on storage root chemistry. | the effects of nitrogen fertilizer on sweet potato, ipomoea batatas (l.) poir., resistance to the sweetpotato weevil, cylas formicarius elegantulus (summers), was studied. adult weevil feeding and oviposition preference, larval survival, and pupal weight were used as measures of sweet potato resistance. sweet potato resin glycosides and caffeic acid concentrations in the periderm tissue of storage roots also were measured. sweet potato genotypes (beauregard, excel, w-244, w-250, and sumor) with ... | 2001 | 11681695 |
| energy expenditure in relation to activity of lesser mouse deer (tragulus javanicus). | heat production (hp) of male and female mouse deer during eating, standing and sitting was determined using the open circuit respiration chamber (rc). the time taken for similar activities was also determined in an outdoor enclosure (od). the animals were fed kangkong (ipomoea aquatica), sweet potato (ipomoea batatas) and rabbit pellet ad libitum. male mouse deer consumed more dry matter (dm), organic matter (om) and gross energy (ge) than female. the time for each activity of male and female mo ... | 2001 | 11691611 |