Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| infection of soybean by cucumber mosaic virus as determined by viral movement protein. | to characterize the host range determinant of the soybean strain of cucumber mosaic virus (cmv) we analyzed a series of pseudorecombinants and chimeric viruses between infectious transcripts from two soybean strains (cmv-sc and cmv-sd) and an ordinary strain (cmv-y). cmv-y could not infect soybeans, even locally. systemic infection of the two soybean-adapted soybean isolates on soybean plants mapped to rna3. chimeric rna3s from between cmv-sc and cmv-y, and chimeric rna3s from between cmv-sc and ... | 2007 | 17006598 |
| genetic spatial clustering: significant implications for conservation of wild soybean (glycine soja: fabaceae). | knowledge of spatial patterns of genetic variation within populations of wild relative species has significant implications with respect to sampling strategies for ex situ and in situ conservation. to study spatial genetic structure of wild soybean (glycine soja sieb. et zucc.) at the fine scale, three natural populations in northern china were analyzed using inter-simple sequence repeat (issr) fingerprints for estimating kinship coefficients. a regression analysis of kinship coefficients agains ... | 2006 | 17028939 |
| qtl associated with horizontal resistance to soybean cyst nematode in glycine soja pi464925b. | soybean cyst nematode (heterodera glycines ichinohe; scn) is the primary disease responsible for yield loss of soybean [glycine max (l.) merr.]. resistant cultivars are an effective management tool; however, the sources currently available have common resistant genes. glycine soja sieb. and zucc., the wild ancestor of domesticated soybean, represents a diverse germplasm pool with known scn resistance. the objectives of this research were to: (1) determine the genetic variation and inheritance of ... | 2007 | 17119912 |
| highly variable patterns of linkage disequilibrium in multiple soybean populations. | prospects for utilizing whole-genome association analysis in autogamous plant populations appear promising due to the reported high levels of linkage disequilibrium (ld). to determine the optimal strategies for implementing association analysis in soybean (glycine max l. merr.), we analyzed the structure of ld in three regions of the genome varying in length from 336 to 574 kb. this analysis was conducted in four distinct groups of soybean germplasm: 26 accessions of the wild ancestor of soybean ... | 2007 | 17287533 |
| [relationship between chloride tolerance and polyamine accumulation in glycine max, glycine soja, and their hybrid seedlings]. | the seedlings of the f4 hybrid strain 'jb185' selected for salt tolerance generation by generation, their parents glycine max cv. jackson and glycine soja population 'bb52' were treated with different nacl concentrations and iso-osmotic (-0.53 mpa) peg-6000, nacl, na+ (without cl-) and cl- (without na+) solutions for 6 d. the results showed that: (1) the relative electrolyte leakage and malondialdehyde (mda) content in leaves of the above three soybean seedlings showed an increase trend when the ... | 2007 | 17287569 |
| proteomic and genetic analysis of glycinin subunits of sixteen soybean genotypes. | we investigated proteomic and genomic profiles of glycinin, a family of major storage proteins in 16 different soybean genotypes consisting of four groups including wild soybean (glycine soja), unimproved cultivated soybean landraces from asia (g. max), ancestors of n. american soybean (g. max), and modern soybean (g. max) genotypes. we observed considerable variation in all five glycinin subunits, g1, g2 g3, g4 and g5 using proteomics and genetic analysis. two-dimensional polyacrylamide gel ele ... | 2016 | 17524657 |
| cloning and functional characterization of an o-acetylserine(thiol)lyase-encoding gene in wild soybean (glycine soja). | the terminal step of soybean cysteine synthesis is catalyzed by o-acetylserine(thiol)lyase (oas-tl, ec 2.5.1.47). in this study, we isolated and characterized an oas-tl gene from a wild soybean material (designated as gsoas-tl1). gsoas-tl1 cdna sequence showed strict conservation at both nucleotide and amino acid levels compared with that from cultivated soybean. genomic structure analysis of gsoas-tl1 indicated that it contained 10 exons and 9 introns in the coding region with conserved exon si ... | 2008 | 17611817 |
| [investigation of genetically modified soybean biosafety in the center of the origin and diversity of russian far east]. | wild soybean (glycine soja sieb. & zucc.) is the nearest relative of a soybean crop (glycine max (l.) merr.). study of population genetic structure of wild-growing relatives ofgenetically modified (gm) plants in the centers of their origin is one of the main procedures before introduction of gm crops in these areas. we have studied genetic variability of nine wild growing soya populations of primorye territory using rapd analysis. the level of g. soja genetic variability was considerably higher ... | 2007 | 17649627 |
| plant community succession in modern yellow river delta, china. | data were collected in different successional stages using a simultaneous sampling method and analyzed through quantitative classification method. three large groups and 12 classes were made to represent the community patterns of three succession stages and 12 succession communities. the succession series of plant community in the study area was as follows: saline bare land-->community suaeda salsa-->community tamarix chinensis-->grassland. succession degree and succession process of 12 successi ... | 2007 | 17657854 |
| [effects of chilling stress under weak light on functions of photosystems in leaves of wild soybean and cultivatar soybean]. | by simultaneously analyzing chlorophyll a fluorescence transient and light absorbance at 820 nm, and detecting activities of superoxide dismutase (sod) and ascorbate peroxidase (apx), the effects of chilling stress under weak light on functions of photosystem i (psi) and photosystem ii (psii) in leaves of a wild type soybean variety 'zyd00019' and a cultivar soybean variety 'zaoshu no.1' were investigated. the results showed that the functions of both psi and psii were damaged by the chilling st ... | 2007 | 17675757 |
| fast-growing rhizobia isolated from root nodules of soybean. | fast-growing rhizobia have been isolated from soybean root nodules collected in china. these new isolates are physiologically distinct from slow-growing soybean rhizobia. they formed effective nitrogen-fixing associations with wild soybean and an unbred soybean cultivar from china, but were largely ineffective as nitrogen-fixing symbionts with common commercial cultivars of soybeans. | 1982 | 17788491 |
| characterization of the soybean genome using est-derived microsatellite markers. | we generated a high-density genetic linkage map of soybean using expressed sequence tag (est)-derived microsatellite markers. a total of 6920 primer pairs (10.9%) were designed to amplify simple sequence repeats (ssrs) from 63,676 publicly available non-redundant soybean ests. the polymorphism of two parent plants, the japanese cultivar 'misuzudaizu' and the chinese line 'moshidou gong 503', were examined using 10% polyacrylamide gel electrophoresis. primer pairs showing polymorphism were then u ... | 2007 | 18192281 |
| an alloantibody to a high-prevalence mns antigen in a person with a gp.jl/mk phenotype. | the low-prevalence mns blood group antigentsen is located at the junction of glycophorin a (gpa) to glycophorin b (gpb) in several hybrid glycophorin molecules. extremely rare people have rbcs with a double dose of the tsen antigen and have made an antibody to a high-prevalence mns antigen. we report the first patient who is heterozygous for gyp.jl and mk. during prenatal tests,an alloantibody to a high-prevalence antigen was detected in the serum of a 21-year-old hispanic woman. the antibody de ... | 2007 | 18284304 |
| 5s rrna genes in tribe phaseoleae: array size, number, and dynamics. | the organization of 5s rrna genes in plants belonging to tribe phaseoleae was investigated by clamped homogeneous electric field gel electrophoresis and southern blot hybridization. representatives of subtribe glycininae included the diploid species neonotonia wightii and teramnus labialis, as well as three soybean accessions: an elite glycine max (l.) merr. cultivar (bsr101), an unadapted g. max introduction (pi 437.654), and a wild glycine soja (pi 468.916). a cultivar of phaseolus vulgaris (k ... | 1996 | 18469906 |
| study of the refirming effect of a plant complex. | loss of skin elasticity is one of the main problems of ageing. this is a mechanical property influenced by elastin, a protein in the dermis which, together with collagen and glycosaminoglycans, makes up the connective tissue. this tissue is affected by a large number of events (such as cutaneous ageing, pregnancy, slimming processes and cellulitis) which eventually cause it to change. at the same time, the metabolism of the proteins of the connective tissue decreases and there is an ever greater ... | 1998 | 18505506 |
| allelic differentiation of kunitz trypsin inhibitor in wild soybean (glycine soja). | soybean kunitz trypsin inhibitor (skti) has several polymorphic types, which are controlled by co-dominant multiple alleles at a single locus. of these types, tia and tib are predominant types, and there are nine differences in amino acids between tia and tib. recently, an intermediate transitional type (tibi5) between them was detected. however, other transitional types have not been detected despite surveys of many cultivated and wild soybeans. one of the reasons why other transitional variant ... | 2008 | 18512041 |
| novel alleles among soybean bowman-birk proteinase inhibitor gene families. | trypsin inhibitors have been found in various animals, plants and microorganisms. there were two types of trypsin inhibitors in soybean including bowman-birk protease inhibitors (bbi) and kunitz inhibitors (kti). the different bbi genes from wild soybean (g. soja) and cultivated soybean (g. max) formed a multigene family. we constructed a cdna library of cultivar 'suinong 14' seed at the r7 growth stage using the smart kit. seventeen contigs or singletons were highly homologous to soybean protea ... | 2008 | 18677596 |
| identification of soybean micrornas and their targets. | the micrornas (mirnas) are a newly identified class of small non-protein-coding regulatory rna. using comparative genomics, we identified 69 mirnas belonging to 33 families in the domesticated soybean (glycine max) as well as five mirnas in the soybean wild species glycine soja and glycine clandestine. taqman((r)) microrna assay analyses demonstrated that these mirnas were differentially expressed in soybean tissues, with certain classes expressed preferentially in both a spatiotemporal and a ti ... | 2008 | 18815805 |
| effect of salt stress on seed germination and seedling growth of three salinity plants. | seeds of three halophyte species included limonium sinense kuntze, glycine soja sieb. and sorghum sudanense stapf. were used to investigate the effects of different salinity concentrations on their germination percentage, germination energy, germination index, relative germination rate, relative salt-injury rate, radicle length and hypocotyl length. results showed that seeds of g. soja can germinate well and rapidly at lower level of salt (< 200 mmol l(-1)), 50 mmol l(-1) salt concentrations is ... | 2008 | 18819537 |
| [molecular genetic analysis of wild soybean (glycine soja sieb. & zucc.) population structure in anthropogenic and natural landscapes of primorskii krai]. | the data are presented on genetic population structure of wild soybean growing in natural and anthropogenically disturbed landscapes of primorskii krai of the russian federation. comparative analysis showed that wild soybean populations exposed to anthropogenic influence exhibited lower genetic diversity than natural populations. recommendations on conservation of the wild plant gene pools using comparative data on population genetic structures are made. | 2008 | 18825957 |
| affinity purification of trypsin inhibitor with anti-aspergillus flavus activity from cultivated and wild soybean. | trypsin inhibitors (ti) from wild-type soybean (glycine soya) (wbti) and domesticated soybean (glycine max) (sbti) were purified using prepared chitosan resin-trypsin as filler on the affinity chromatography column. the sbti/wbti purification fold by affinity chromatography was 718- and 279-fold, with the activity recovery of 62% and 59%, respectively. it was found that sbti and wbti exerted a strong inhibition of aspergillus. flavus growth, with ic(50) of 1.6 and 1.0 micromol/l. this growth inh ... | 2009 | 18830687 |
| gs52 ecto-apyrase plays a critical role during soybean nodulation. | apyrases are non-energy-coupled nucleotide phosphohydrolases that hydrolyze nucleoside triphosphates and nucleoside diphosphates to nucleoside monophosphates and orthophosphates. gs52, a soybean (glycine soja) ecto-apyrase, was previously shown to be induced very early in response to inoculation with the symbiotic bacterium bradyrhizobium japonicum. overexpression of the gs52 ecto-apyrase in lotus japonicus increased the level of rhizobial infection and enhanced nodulation. these data suggest a ... | 2009 | 19036836 |
| utility of proteomics techniques for assessing protein expression. | proteomic technologies are currently used as an effective analytical tool for examining modifications in protein profiles. understanding the natural variation of soybean seed proteins is necessary to evaluate potential unintended (collateral) effects due to transgenic modifications in genetically modified (gmo) soybeans. we used two-dimensional polyacrylamide gel electrophoresis (2d-page), matrix-assisted laser desorption/ionization time of flight (maldi-tof) mass spectrometry, and liquid chroma ... | 2009 | 19133307 |
| genetic diversity and peculiarity of annual wild soybean (g. soja sieb. et zucc.) from various eco-regions in china. | annual wild soybean (glycine soja sieb. et zucc.) is believed to be a potential gene source for future soybean improvement in coping with the world climate change for food security. to evaluate the wild soybean genetic diversity and differentiation, we analyzed allelic profiles at 60 simple-sequence repeat (ssr) loci and variation of eight morph-biological traits of a representative sample with 196 accessions from the natural growing area in china. for comparison, a representative sample with 20 ... | 2009 | 19449177 |
| inheritance of salt tolerance in wild soybean (glycine soja sieb. and zucc.) accession pi483463. | tolerant soybean (glycine max [l.] merr.) cultivars aid in reducing salt damage in problem fields. new genes are important to reduce losses from salt injury. objectives of this study were to determine inheritance of salt tolerance in wild soybean (glycine soja sieb. and zucc.) pi483463 and to test allelism of tolerance genes from genotypes pi483463 and s-100, a common ancestor of southern in us cultivars. tolerant (t) pi483463 was crossed to sensitive (s) cultivar hutcheson to study inheritance. ... | 2009 | 19451208 |
| ensifer meliloti bv. lancerottense establishes nitrogen-fixing symbiosis with lotus endemic to the canary islands and shows distinctive symbiotic genotypes and host range. | eleven strains were isolated from root nodules of lotus endemic to the canary islands and they belonged to the genus ensifer, a genus never previously described as a symbiont of lotus. according to their 16s rrna and atpd gene sequences, two isolates represented minority genotypes that could belong to previously undescribed ensifer species, but most of the isolates were classified within the species ensifer meliloti. these isolates nodulated lotus lancerottensis, lotus corniculatus and lotus jap ... | 2009 | 19477097 |
| biological safety of liposome-fullerene consisting of hydrogenated lecithin, glycine soja sterols, and fullerene-c60 upon photocytotoxicity and bacterial reverse mutagenicity. | various water-soluble derivatives of fullerene-c60 (c60) have been developed as detoxifiers for reactive oxygen species (ros), whereas c60 incorporated in liposome (lpsm) has not been reported yet. we prepared the liposome-fullerene (0.2% aqueous phase, lpsm-flln) which was composed of hydrogenated lecithin, glycine soja (soybean) sterols, and c60 in the weight ratio of 89.7:10:0.3, then examined the photocytotoxicity and bacterial reverse mutagenicity, as comparing with the lpsm containing no c ... | 2009 | 19482914 |
| effects of nacl stress on antioxidative enzymes of glycine soja sieb. | the activity of anti-oxidant enzymes (superoxide dismutase (sod), peroxidase (pod), catalase (cat) and parameters of oxidative stress malondialdehyde (mda) of shoots were investigated in s. sieb naturally salt-resistant halophyte. the seedlings of s. sieb were treated with varying (0, 80, 160 and 240 mm) nacl stress. the results showed that nacl played an important role in growth of s. sieb. it made obviously promotion of certain nacl concentration to growth of s. sieb, the seeflings of s. sieb ... | 2009 | 19580000 |
| molecular and chromosomal evidence for allopolyploidy in soybean. | recent studies have documented that the soybean (glycine max) genome has undergone two rounds of large-scale genome and/or segmental duplication. to shed light on the timing and nature of these duplication events, we characterized and analyzed two subfamilies of high-copy centromeric satellite repeats, centgm-1 and centgm-2, using a combination of computational and molecular cytogenetic approaches. these two subfamilies of satellite repeats mark distinct subsets of soybean centromeres and, in at ... | 2009 | 19605552 |
| identification of micrornas in wild soybean (glycine soja). | micrornas (mirnas) play important roles in post-transcriptional gene silencing by directing target mrna cleavage or translational inhibition. currently, hundreds of mirnas have been identified in plants, but no report has been published of wild soybean (glycine soja sieb). we constructed a small-rna library consisting of 2 880 sequences with high quality, in which 1 347 were 19-24 nt in length. by utilizing the mirna, rfam and domesticated soybean expressed sequence tag database, we have analyze ... | 2009 | 20021554 |
| fullerene-c60/liposome complex: defensive effects against uva-induced damages in skin structure, nucleus and collagen type i/iv fibrils, and the permeability into human skin tissue. | we previously reported biological safety of fullerene-c60 (c60) incorporated in liposome consisting of hydrogenated lecithin and glycine soja sterol, as liposome-fullerene (0.5% aqueous phase; a particle size, 76nm; lpsm-flln), and its cytoprotective activity against uva. in the present study, lpsm-flln was administered on the surface of three-dimensional human skin tissue model, rinsed out before each uva-irradiation at 4 j/cm(2), and thereafter added again, followed by 19-cycle-repetition for ... | 2010 | 20036139 |
| [genetic variation of snp loci based on candidate gene for resistance to soybean cyst nematode]. | for clarifying the difference of genetic diversity and linkage disequilibrium (ld) level between cultivated (glycine max (l.) merr.) and annual wild soybean (glycine soja sieb. & zucc.), genetic variation pattern of 8 snp loci developed from soybean cyst nematode resistance candidate genes rhg1 and rhg4 in soybean germplasm were analyzed. the results indicated that g. max population, consisted of cultivated soybean mini-core collection and modern cultivars, had a higher ld levels (r2 value is 0. ... | 2009 | 20042394 |
| identification of a major qtl allele from wild soybean (glycine soja sieb. & zucc.) for increasing alkaline salt tolerance in soybean. | salt-affected soils are generally classified into two main categories, sodic (alkaline) and saline. our previous studies showed that the wild soybean accession jws156-1 (glycine soja) from the kinki area of japan was tolerant to nacl salt, and the quantitative trait locus (qtl) for nacl salt tolerance was located on soybean linkage group n (chromosome 3). further investigation revealed that the wild soybean accession jws156-1 also had a higher tolerance to alkaline salt stress. in the present st ... | 2010 | 20204319 |
| over-expression of a glutathione s-transferase gene, gsgst, from wild soybean (glycine soja) enhances drought and salt tolerance in transgenic tobacco. | glycine soja is a species of soybean that survives in adverse environments including high salt and drought conditions. we constructed a cdna library from g. soja seedlings treated with nacl and isolated a glutathione s-transferase gene (gsgst: gq265911) from the library. the cdna encoding gsgst contains an open reading frame of 660 bp and the predicted protein belongs to the tau class of gst family proteins. tobacco plants over-expressing the gsgst gene showed sixfold higher gst activity than wi ... | 2010 | 20383560 |
| gscbrlk, a calcium/calmodulin-binding receptor-like kinase, is a positive regulator of plant tolerance to salt and aba stress. | calcium/calmodulin-dependent kinases play vital roles in protein phosphorylation in eukaryotes, yet little is known about the phosphorylation process of calcium/calmodulin-dependent protein kinase and its role in stress signal transduction in plants. a novel plant-specific calcium-dependent calmodulin-binding receptor-like kinase (gscbrlk) has been isolated from glycine soja. a subcellular localization study using gfp fusion protein indicated that gscbrlk is localized in the plasma membrane. bin ... | 2010 | 20400529 |
| clinical efficacy comparison of anti-wrinkle cosmetics containing herbal flavonoids. | herbal anti-wrinkle cosmetics were formulated from ginkgo (ginkgo biloba), a mixture of tea and rooibos (camellia sinensis and aspalathus linearis) and soybean (glycine soja). these extracts were incorporated into the preliminary developed stable gel base with good preference. the gingko formulation was found to be more stable than the formula containing a mixture of tea and rooibos and the soybean formula. clinical efficacies of the ginkgo formula and the formula containing a mixture of tea and ... | 2010 | 20412217 |
| artificial selection for determinate growth habit in soybean. | determinacy is an agronomically important trait associated with the domestication in soybean (glycine max). most soybean cultivars are classifiable into indeterminate and determinate growth habit, whereas glycine soja, the wild progenitor of soybean, is indeterminate. indeterminate (dt1/dt1) and determinate (dt1/dt1) genotypes, when mated, produce progeny that segregate in a monogenic pattern. here, we show evidence that dt1 is a homolog (designated as gmtfl1) of arabidopsis terminal flower 1 (t ... | 2010 | 20421496 |
| a fluorescence in situ hybridization system for karyotyping soybean. | the development of a universal soybean (glycine max [l.] merr.) cytogenetic map that associates classical genetic linkage groups, molecular linkage groups, and a sequence-based physical map with the karyotype has been impeded due to the soybean chromosomes themselves, which are small and morphologically homogeneous. to overcome this obstacle, we screened soybean repetitive dna to develop a cocktail of fluorescent in situ hybridization (fish) probes that could differentially label mitotic chromos ... | 2010 | 20421607 |
| the origin and fate of morphological intermediates between wild and cultivated soybeans in their natural habitats in japan. | the spread of transgenes into the genome of wild soybean is a concern when transgenic and wild soybeans are planted sympatrically. the objectives of this study were to investigate the origin and fate of morphological intermediates between wild and cultivated soybeans in their natural habitats in japan. twenty nuclear microsatellite and two chloroplast dcaps markers were used to evaluate genetic variation of 468 wild, 17 intermediate, and 12 cultivated soybean samples collected from six sites bet ... | 2010 | 20444080 |
| a single origin and moderate bottleneck during domestication of soybean (glycine max): implications from microsatellites and nucleotide sequences. | background and aims it is essential to illuminate the evolutionary history of crop domestication in order to understand further the origin and development of modern cultivation and agronomy; however, despite being one of the most important crops, the domestication origin and bottleneck of soybean (glycine max) are poorly understood. in the present study, microsatellites and nucleotide sequences were employed to elucidate the domestication genetics of soybean. methods the genomes of 79 landrace s ... | 2010 | 20566681 |
| comparison of genetic diversity between canadian adapted genotypes and exotic germplasm of soybean. | soybean (glycine max (l.) merr.) was domesticated in china and the greatest genetic diversity for this species is found in asia. in contrast, in north america, soybean cultivars trace back to a small number of plant introductions from asia and genetic diversity is typically quite limited. the purpose of this work was to measure and compare the genetic diversity in two sets of soybean lines. the first set (termed "local") was composed of 100 lines used in a private breeding program in quebec. the ... | 2010 | 20616865 |
| genetic diversity in domesticated soybean (glycine max) and its wild progenitor (glycine soja) for simple sequence repeat and single-nucleotide polymorphism loci. | • the study of genetic diversity between a crop and its wild relatives may yield fundamental insights into evolutionary history and the process of domestication. • in this study, we genotyped a sample of 303 accessions of domesticated soybean (glycine max) and its wild progenitor glycine soja with 99 microsatellite markers and 554 single-nucleotide polymorphism (snp) markers. • the simple sequence repeat (ssr) loci averaged 21.5 alleles per locus and overall nei's gene diversity of 0.77. the snp ... | 2010 | 20618914 |
| global transcriptome profiling of wild soybean (glycine soja) roots under nahco3 treatment. | plant roots are the primary site of perception and injury for saline-alkaline stress. the current knowledge of saline-alkaline stress transcriptome is mostly focused on saline (nacl) stress and only limited information on alkaline (nahco3) stress is available. | 2010 | 20653984 |
| a new allele of flower color gene w1 encoding flavonoid 3'5'-hydroxylase is responsible for light purple flowers in wild soybean glycine soja. | glycine soja is a wild relative of soybean that has purple flowers. no flower color variant of glycine soja has been found in the natural habitat. | 2010 | 20663233 |
| natural variability in abundance of prevalent soybean proteins. | soybean is an inexpensive source of protein for humans and animals. genetic modifications (gmo) to soybean have become inevitable on two fronts, both quality and yield will need to improve to meet increasing global demand. to ensure the safety of the crop for consumers it is important to determine the natural variation in seed protein constituents as well as any unintended changes that may occur in the gmo as a result of genetic modification. understanding the natural variation of seed proteins ... | 2010 | 20709130 |
| ensifer (sinorhizobium) sojae sp. nov., isolated from root nodules of glycine max grown in saline-alkaline soils in hebei province of china. | thirteen bacterial strains isolated from root nodules of soybean grown in saline-alkaline soils in the chinese province of hebei were identified as a unique group in genus ensifer (sinorhizobium) based upon box-pcr patterns, sequencing analyses of 16s rrna and housekeeping genes, and dna-dna hybridization. phenotypically, positive acid production and negative reduction in litmus milk and sensitivity to 50 µg ml(1) of amplicillin, as well as some other features could differentiate the novel group ... | 2010 | 20851917 |
| phenolics in the seed coat of wild soybean (glycine soja) and their significance for seed hardness and seed germination. | hardseededness in annual wild soybean (glycine soja sieb. et zucc.) is a valuable trait that affects the germination, viability, and quality of stored seeds. two g. soja ecotypes native to shandong province of china have been used to identify the phenolics in the seed coat that correlate with the seed hardness and seed germination. three major phenolics from the seed coat were isolated and identified as epicatechin, cyanidin 3-o-glucoside, and delphinidin 3-o-glucoside. of the three phenolics, o ... | 2010 | 20879792 |
| alkaline-stress response in glycine soja leaf identifies specific transcription factors and aba-mediated signaling factors. | transcriptome of glycine soja leaf tissue during a detailed time course formed a foundation for examining transcriptional processes during nahco(3) stress treatment. of a total of 2,310 detected differentially expressed genes, 1,664 genes were upregulated and 1,704 genes were downregulated at various time points. the number of stress-regulated genes increased dramatically after a 6-h stress treatment. go category gene enrichment analysis revealed that most of the differentially expressed genes w ... | 2011 | 20938706 |
| resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection. | we report a large-scale analysis of the patterns of genome-wide genetic variation in soybeans. we re-sequenced a total of 17 wild and 14 cultivated soybean genomes to an average of approximately ×5 depth and >90% coverage using the illumina genome analyzer ii platform. we compared the patterns of genetic variation between wild and cultivated soybeans and identified higher allelic diversity in wild soybeans. we identified a high level of linkage disequilibrium in the soybean genome, suggesting th ... | 2010 | 21076406 |
| hybridization between gm soybean (glycine max (l.) merr.) and wild soybean (glycine soja sieb. et zucc.) under field conditions in japan. | accumulation of information about natural hybridization between gm soybean (glycine max) and wild soybean (glycine soja) is required for risk assessment evaluation and to establish biosafety regulations in japan. this is particularly important in areas where wild relatives of cultivated soybean are grown (i.e. east asia including japan). to collect information on temporal and spatial factors affecting variation in hybridization between wild and gm soybean, a two year hybridization experiment was ... | 2010 | 21122483 |
| whole-genome sequencing and intensive analysis of the undomesticated soybean (glycine soja sieb. and zucc.) genome. | the genome of soybean (glycine max), a commercially important crop, has recently been sequenced and is one of six crop species to have been sequenced. here we report the genome sequence of g. soja, the undomesticated ancestor of g. max (in particular, g. soja var. it182932). the 48.8-gb illumina genome analyzer (illumina-ga) short dna reads were aligned to the g. max reference genome and a consensus was determined for g. soja. this consensus sequence spanned 915.4 mb, representing a coverage of ... | 2010 | 21131573 |
| genetic diversity of nodulating and non-nodulating rhizobia associated with wild soybean (glycine soja sieb. & zucc.) in different ecoregions of china. | a total of 99 bacterial isolates that originated from root nodules of glycine soja were characterized with restriction analyses of amplified 16s ribosomal dna and 16s-23s rdna intergenic spacers (its), and sequence analyses of 16s rrna, rpob, atpd, reca and nodc genes. when tested for nodulation of g. soja, 72 of the isolates were effective symbionts, and these belonged to five species: bradyrhizobium japonicum, bradyrhizobium elkanii, bradyrhizobium yuanmingense, bradyrhizobium liaoningense and ... | 2011 | 21303397 |
| treatment of bromhidrosis with a glycine-soja sterocomplex topical product. | bromhidrosis is a common problem with a severe negative impact on the patient's social and emotional life. the objective of this open-label study was to evaluate the efficacy and safety of a local product with the main active ingredient of glycine-soja sterocomplex in the treatment of local bromhidrosis. | 2011 | 21332920 |
| a novel glycine soja tonoplast intrinsic protein gene responds to abiotic stress and depresses salt and dehydration tolerance in transgenic arabidopsis thaliana. | tonoplast intrinsic protein (tip) is a subfamily of the aquaporin (aqp), also known as major intrinsic protein (mip) family, and regulates water movement across vacuolar membranes. some reports have implied that tip genes are associated with plant tolerance to some abiotic stresses that cause water loss, such as drought and high salinity. in our previous work, we found that an expressed sequence tag (est) representing a tip gene in our glycine soja est library was inducible by abiotic stresses. ... | 2011 | 21397356 |
| [study of genetic diversity and spatial structure of the wild soybean (glycine soja sieb. & zucc.) population from the ekaterinovka in the south of primorskii krai]. | data are presented on the genetic diversity and spatial structure of the natural wild soybean population from the neighborhood of the settlement of ekaterinovka in primorskii krai and on the relationship between the genetic structure of this population and its spatial organization. these data are discussed in comparison with the results of studies of wild soybean populations in the far east region of the russian federation and china. recommendations are given concerning the collection of genetic ... | 2011 | 21542304 |
| a model to predict the frequency of integration of fitness-related qtls from cultivated to wild soybean. | with the proliferation of genetically modified (gm) products and the almost exponential growth of land use for gm crops, there is a growing need to develop quantitative approaches to estimating the risk of escape of transgenes into wild populations of crop relatives by natural hybridization. we assessed the risk of transgene escape by constructing a population genetic model based on information on fitness-related qtls obtained from an f (2) population of wild soybean g. soja × cultivated soybean ... | 2012 | 21544624 |
| secondary metabolites from glycine soja and their growth inhibitory effect against spodoptera litura. | the wild soybean (glycine soja sieb. et zucc) has been reported to be relatively resistant to insect and pathogenic pests. however, the responsible secondary metabolites in the aerial part of this important plant are largely unknown. from the aerial part of g. soja, 13 compounds were isolated and identified, including seven isoflavonoids (1-7), a cyclitol (8), two sterol derivatives (9 and 10), and three triterpenoids (11-13). compound 7 is a new isoflavonoid, and compounds 9 and 10 are reported ... | 2011 | 21545185 |
| fine-scale genetic structure enhances biparental inbreeding by promoting mating events between more related individuals in wild soybean (glycine soja; fabaceae) populations. | outcrossing between genetically distant individuals in a plant population enhances allelic heterozygosity-an important source for genetic diversity and adaptive evolution. fine-scale spatial genetic structure (fsgs) can interfere with outcrossing by promoting mating between more related individuals. to test the influence of fsgs on outcrossing, fsgs and outcrossing rates were analyzed with four wild soybean (glycine soja) populations from different habitats, using simple sequence repeat (ssr) fi ... | 2009 | 21628264 |
| fullerene-c60 incorporated in liposome exerts persistent hydroxyl radical-scavenging activity and cytoprotection in uva/b-irradiated keratinocytes. | the aim of this study is to examine antioxidant activity of fullerene-c60 (c60) incorporated in liposome (lpsmflln, a diameter of 75.6 nm). lpsmflln is water-soluble, and composed of hydrogenated lecithin of 89.7%, glycine soja sterol of 10% and c60 of 0.3%. hydroxyl radicals (*oh), generated from uva- or uvb-irradiated h2o2, were scavenged by lpsmflln but not by c60-lacking lpsm as assessed by esr, showing that the active principle is c60 as scanty as 1/415 weight versus lpsmflln; the *oh amoun ... | 2011 | 21780373 |
| gstify10, a novel positive regulator of plant tolerance to bicarbonate stress and a repressor of jasmonate signaling. | recent discoveries show that tify family genes are plant-specific genes involved in the response to several abiotic stresses, also acting as key regulators of jasmonate signaling in arabidopsis thaliana. however, there is limited information about this gene family in wild soybean, nor is its role in plant bicarbonate stress adaptation completely understood. here, we isolated and characterized a novel tify family gene, gstify10, from glycine soja. gstify10 could be induced by bicarbonate, salinit ... | 2011 | 21805375 |
| gsgasa1 mediated root growth inhibition in response to chronic cold stress is marked by the accumulation of dellas. | low, but not freezing temperatures are a major factor limiting plant vegetative growth and development. it is not clear what signaling mechanism is used to limit plant growth in response to chronic low temperatures, thus it is important to continue to isolate and characterize genes whose activity/expression correlate well with cold responses. in this study, a novel gasa gene was isolated from glycine soja and named gsgasa1. quantitative real-time pcr analysis indicated that gsgasa1 expression re ... | 2011 | 21855169 |
| [study of interaction of wild soybean subpopulations (glycine soja) in the valley of the tsukanovka river in the south of far east of russia]. | a comparative study of the genetic structure of natural and anthropogenic populations of g. soja gives significant information about formation of different populations, and allows developing measures for preservation of unique natural gene bank of wild soybean, the species closely related to cultivated soybean. in this study, issr markers were used to carry out a comparative analysis of genetic structure of natural and anthropogenic subpopulations of g. soja for studying possible mutual influenc ... | 2011 | 21950138 |
| fine-scale phylogenetic structure and major events in the history of the current wild soybean (glycine soja) and taxonomic assignment of semi-wild type (glycine gracilis skvortz.) within the chinese subgenus soja. | wild and cultivated species of soybeans have coexisted for 5000 years in china. despite this long history, there is very little information on the genetic relationship of glycine soja and g. max. to gain insight into the major events in the history of the subgenus soja, we examined 20 simple sequence repeat (ssr) markers of a large number of accessions (910). the results showed no significant differences between wild and semi-wild soybeans in genetic diversity but significant differences between ... | 2015 | 21984661 |
| bradyrhizobium huanghuaihaiense sp. nov., an effective symbiotic bacterium isolated from soybean (glycine max l.) nodules in northern (huang-huai-hai) plain of china. | in a survey of biodiversity and biogeography of rhizobia associated with soybean (glycine max l.) in different sites of northern (huang-huai-hai) plain of china, ten strains were defined as a novel genomic species in the genus of bradyrhizobium. they were distinguished from defined species in rflp (restriction fragment length polymorphism) of 16s rrna gene and intergenic spacer (igs) between 16s and 23s rrna genes. in box-pcr, these strains presented two patterns sharing 94% similarities, demons ... | 2011 | 22003042 |
| phylogenetic relationship of lotus uliginosus symbionts with bradyrhizobia nodulating genistoid legumes. | lotus species are legumes with potential for pastures in soils with low-fertility and environmental constraints. the aim of this work was to characterize bacteria that establish efficient nitrogen-fixing symbiosis with the forage species lotus uliginosus. a total of 39 isolates were obtained from nodules of l. uliginosus naturally growing in two different locations of portugal. molecular identification of the isolates plus the commercial inoculant strain nzp2039 was performed by rep-pcr, 16s rrn ... | 2011 | 22092879 |
| The ß-conglycinin deficiency in wild soybean is associated with the tail-to-tail inverted repeat of the a-subunit genes. | ß-Conglycinin, a major seed protein in soybean, is composed of a, a', and ß subunits sharing a high homology among them. Despite its many health benefits, ß-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the a, a', and ß subunits also contain major allergens. A wild soybean (Glycine soja Sieb et Zucc.) line, 'QT2', lacks all of the ß-conglycinin subunits, and the deficien ... | 2011 | 22193750 |
| pericentromeric effects shape the patterns of divergence, retention, and expression of duplicated genes in the paleopolyploid soybean. | the evolutionary forces that govern the divergence and retention of duplicated genes in polyploids are poorly understood. in this study, we first investigated the rates of nonsynonymous substitution (ka) and the rates of synonymous substitution (ks) for a nearly complete set of genes in the paleopolyploid soybean (glycine max) by comparing the orthologs between soybean and its progenitor species glycine soja and then compared the patterns of gene divergence and expression between pericentromeric ... | 2012 | 22227891 |
| polymorphism of dna sequences of cryptochrome genes is not associated with the photoperiodic flowering of wild soybean along a latitudinal cline. | both cultivated soybean and its wild relative glycine soja exhibit strong photoperiodic sensitivity at different latitudes. recent studies have demonstrated that the blue light-absorbing cryptochrome gene, cry1a, is involved in the photoperiodic flowering of soybeans. however, no sequence variation was found in the cdna among cultivars at different latitudinal clines. in the present study, we examined whether positive selection due to polymorphisms in the cryptochrome genes of g. soja occurs. pa ... | 2012 | 22252378 |
| [isolation and functional analysis of gstify11b relevant to salt and alkaline stress from glycine soja]. | using homologous cloning and rt-pcr technology, we isolated a novel tify family gene, gstify11b, from glycine soja l. g07256, a species that is tolerant to saline and alkaline environments. phylogenetic analysis indicated that gstify11b was closely related to attify11a with 56% similarity in amino acid identity. protein sequence analysis showed that gstify11b protein also had conserved tify domain, n-terminal domain, and a c-terminal jas motif. quantitative realtime pcr analysis indicated that t ... | 2012 | 22382065 |
| integration of the draft sequence and physical map as a framework for genomic research in soybean (glycine max (l.) merr.) and wild soybean (glycine soja sieb. and zucc.). | soybean is a model for the legume research community because of its importance as a crop, densely populated genetic maps, and the availability of a genome sequence. even though a whole-genome shotgun sequence and bacterial artificial chromosome (bac) libraries are available, a high-resolution, chromosome-based physical map linked to the sequence assemblies is still needed for whole-genome alignments and to facilitate map-based gene cloning. three independent g. max bac libraries combined with ge ... | 2012 | 22413085 |
| gsapk, an aba-activated and calcium-independent snrk2-type kinase from g. soja, mediates the regulation of plant tolerance to salinity and aba stress. | plant snf1 (sucrose non-fermenting-1) related protein kinase (snrk), a subfamily of serine/threonine kinases, has been implicated as a crucial upstream regulator of aba and osmotic signaling as in many other signaling cascades. in this paper, we have isolated a novel plant specific aba activated calcium independent protein kinase (gsapk) from a highly salt tolerant plant, glycine soja (50109), which is a member of the snrk2 family. subcellular localization studies using gfp fusion protein indica ... | 2012 | 22439004 |
| comparative genomic analysis of soybean flowering genes. | flowering is an important agronomic trait that determines crop yield. soybean is a major oilseed legume crop used for human and animal feed. legumes have unique vegetative and floral complexities. our understanding of the molecular basis of flower initiation and development in legumes is limited. here, we address this by using a computational approach to examine flowering regulatory genes in the soybean genome in comparison to the most studied model plant, arabidopsis. for this comparison, a gen ... | 2012 | 22679494 |
| [progress in genes related to seed-coat color in soybean]. | seed-coat color has changed from black to yellow during natural and artificial selection of cultivated soybean from wild soybean, and it is also an important morphological marker. therefore, discovering genes related to the soybean seed-coat color will play a very important role in breeding and evolutionary study. different seed-coat colors caused by deposition of various anthocyanin pigments. although pigmentation has been well dissected at molecular level in several plant species, the genes co ... | 2012 | 22698739 |
| over-expression of gszfp1, an aba-responsive c2h2-type zinc finger protein lacking a qalggh motif, reduces aba sensitivity and decreases stomata size. | a cdna of the gene gszfp1 was cloned from glycine soja. gszfp1 encodes a protein with one c2h2-type zinc finger motif. the qalggh motif, which exists in most plant c2h2-type zinc finger proteins (zfps), does not exist in gszfp1. real-time rt-pcr revealed that gszfp1 expression was significantly up-regulated by exogenous aba, both in leaves and roots. over-expression of this gene, in arabidopsis thaliana, resulted in a reduced sensitivity to aba during seed germination and seedling growth. transc ... | 2012 | 22705253 |
| population structure of the wild soybean (glycine soja) in china: implications from microsatellite analyses. | wild soybean (glycine soja), a native species of east asia, is the closest wild relative of the cultivated soybean (g. max) and supplies valuable genetic resources for cultivar breeding. analyses of the genetic variation and population structure of wild soybean are fundamental for effective conservation studies and utilization of this valuable genetic resource. | 2012 | 22791743 |
| recent developments of genomic research in soybean. | soybean is an important cash crop with unique and important traits such as the high seed protein and oil contents, and the ability to perform symbiotic nitrogen fixation. a reference genome of cultivated soybeans was established in 2010, followed by whole-genome re-sequencing of wild and cultivated soybean accessions. these efforts revealed unique features of the soybean genome and helped to understand its evolution. mapping of variations between wild and cultivated soybean genomes were performe ... | 2012 | 22835978 |
| dna sequence polymorphism of the rhg4 candidate gene conferring resistance to soybean cyst nematode in chinese domesticated and wild soybeans. | rhg4 is one of the major resistant genes conferring resistance to soybean cyst nematode races 1, 3 and 4. in order to better understand its sequence diversity among different chinese soybean populations and the impact of human activities on it, we designed 5 primer sets based on its sequence deposited in genbank (genbank accession no. af506518) to obtain the rhg4 sequence from 104 chinese cultivated and wild soybean genotypes, and then analyzed the dna sequence polymorphism in different chinese ... | 2012 | 22924021 |
| over-expression of a novel jaz family gene from glycine soja, increases salt and alkali stress tolerance. | salt and alkali stress are two of the main environmental factors limiting crop production. recent discoveries show that the jaz family encodes plant-specific genes involved in jasmonate signaling. however, there is only limited information about this gene family in abiotic stress response, and in wild soybean (glycine soja), which is a species noted for its tolerance to alkali and salinity. here, we isolated and characterized a novel jaz family gene, gsjaz2, from g. soja. transcript abundance of ... | 2012 | 22943855 |
| temperature interactions with transpiration response to vapor pressure deficit among cultivated and wild soybean genotypes. | a key strategy in soybean drought research is increased stomatal sensitivity to high vapor pressure deficit (vpd), which contributes to the 'slow wilting' trait observed in the field. these experiments examined whether temperature of the growth environment affected the ability of plants to respond to vpd, and thus control transpiration rate (tr). two soybean [glycine max (l.) merr.] and four wild soybean [glycine soja (sieb. and zucc.)] genotypes were studied. the tr was measured over a range of ... | 2013 | 22989317 |
| identification of wild soybean mirnas and their target genes responsive to aluminum stress. | micrornas (mirnas) play important regulatory roles in development and stress response in plants. wild soybean (glycine soja) has undergone long-term natural selection and may have evolved special mechanisms to survive stress conditions as a result. however, little information about mirnas especially mirnas responsive to aluminum (al) stress is available in wild soybean. | 2012 | 23040172 |
| identification of wild soybean (glycine soja) tify family genes and their expression profiling analysis under bicarbonate stress. | wild soybean (glycine soja l. g07256) exhibits a greater adaptability to soil bicarbonate stress than cultivated soybean, and recent discoveries show that tify family genes are involved in the response to several abiotic stresses. a genomic and transcriptomic analysis of all tify genes in g. soja, compared with g. max, will provide insight into the function of this gene family in plant bicarbonate stress response. this article identified and characterized 34 tify genes in g. soja. sequence analy ... | 2013 | 23090726 |
| tracing soybean domestication history: from nucleotide to genome. | since the genome sequences of wild species may provide key information about the genetic elements involved in speciation and domestication, the undomesticated soybean (glycine soja sieb. and zucc.), a wild relative of the current cultivated soybean (g. max), was sequenced. in contrast to the current hypothesis of soybean domestication, which holds that the current cultivated soybean was domesticated from g. soja, our previous work has suggested that soybean was domesticated from the g. soja/g. m ... | 2012 | 23136484 |
| the national bioresource project (nbrp) lotus and glycine in japan. | the objective of the national bioresource project (nbrp) in japan is to collect, conserve and distribute biological materials for life sciences research. the project consists of twenty-eight bioresources, including animal, plant, microorganism and dna resources. nbrp lotus and glycine aims to support the development of legume research through the collection, conservation, and distribution of these bioresources. lotus japonicus is a perennial legume that grows naturally throughout japan and is wi ... | 2012 | 23136485 |
| genome-wide genetic dissection of germplasm resources and implications for breeding by design in soybean. | "breeding by design" as a concept described by peleman and van der voort aims to bring together superior alleles for all genes of agronomic importance from potential genetic resources. this might be achievable through high-resolution allele detection based on precise qtl (quantitative trait locus/loci) mapping of potential parental resources. the present paper reviews the works at the chinese national center for soybean improvement (ncsi) on exploration of qtl and their superior alleles of agron ... | 2012 | 23136489 |
| mapping and use of qtls controlling pod dehiscence in soybean. | while the cultivated soybean, glycine max (l.) merr., is more recalcitrant to pod dehiscence (shattering-resistant) than wild soybean, glycine soja sieb. & zucc., there is also significant genetic variation in shattering resistance among cultivated soybean cultivars. to reveal the genetic basis and develop dna markers for pod dehiscence, several research groups have conducted quantitative trait locus (qtl) analysis using segregated populations derived from crosses between g. max accessions or be ... | 2012 | 23136494 |
| genetic studies on saline and sodic tolerances in soybean. | salt-affected soils are generally classified into two main categories: saline and sodic (alkaline). developing and using soybean (glycine max (l.) merr) cultivars with high salt tolerance is an effective way of maintaining sustainable production in areas where soybean growth is threatened by salt stress. early classical genetics studies revealed that saline tolerance was conditioned by a single dominant gene. recently, a series of studies consistently revealed a major quantitative trait locus (q ... | 2012 | 23136495 |
| evaluation of soybean germplasm conserved in nias genebank and development of mini core collections. | genetic variation and population structure among 1603 soybean accessions, consisted of 832 japanese landraces, 109 old and 57 recent japanese varieties, 341 landrace from 16 asian countries and 264 wild soybean accessions, were characterized using 191 snp markers. although gene diversity of japanese soybean germplasm was slight lower than that of exotic soybean germplasm, population differentiation and clustering analyses indicated clear genetic differentiation among japanese cultivated soybeans ... | 2012 | 23136496 |
| genetic analysis of variations in the sugar chain composition at the c-3 position of soybean seed saponins. | saponins are sterols or triterpene glycosides that are widely distributed in plants. the biosynthesis of soybean saponins is thought to involve many kinds of glycosyltransferases, which is reflected in their structural diversity. here, we performed linkage analyses of the sg-3 and sg-4 loci, which may control the sugar chain composition at the c-3 sugar moieties of the soybean saponin aglycones soyasapogenols a and b. the sg-3 locus, which controls the production of group a saponin af, was mappe ... | 2012 | 23136503 |
| genetic diversity and population structure: implications for conservation of wild soybean (glycine soja sieb. et zucc) based on nuclear and chloroplast microsatellite variation. | wild soybean (glycine soja sieb. et zucc) is the most important germplasm resource for soybean breeding, and is currently subject to habitat loss, fragmentation and population decline. in order to develop successful conservation strategies, a total of 604 wild soybean accessions from 43 locations sampled across its range in china, japan and korea were analyzed using 20 nuclear (nssrs) and five chloroplast microsatellite markers (cpssrs) to reveal its genetic diversity and population structure. r ... | 2012 | 23202917 |
| uncovering the salt response of soybean by unraveling its wild and cultivated functional genomes using tag sequencing. | soil salinity has very adverse effects on growth and yield of crop plants. several salt tolerant wild accessions and cultivars are reported in soybean. functional genomes of salt tolerant glycine soja and a salt sensitive genotype of glycine max were investigated to understand the mechanism of salt tolerance in soybean. for this purpose, four libraries were constructed for tag sequencing on illumina platform. we identify around 490 salt responsive genes which included a number of transcription f ... | 2012 | 23209559 |
| genetic and chemical analysis of a key biosynthetic step for soyasapogenol a, an aglycone of group a saponins that influence soymilk flavor. | although certain saponins in soybean seeds have been reported to have health benefits, group a acetyl saponins cause undesirable bitter and astringent tastes in soy products. therefore, reduction or elimination of group a saponins is an important target for soybean breeders. a wide survey of cultivated and wild soybean germplasm identified a mutant line that lacked group a saponins. the absence of soyasapogenol a, a group a saponin aglycone, is controlled by a single recessive allele, sg-5 that ... | 2013 | 23229125 |
| gssrk, a g-type lectin s-receptor-like serine/threonine protein kinase, is a positive regulator of plant tolerance to salt stress. | receptor-like protein kinases (rlks) play vital roles in sensing outside signals, yet little is known about rlks functions and roles in stress signal perception and transduction in plants, especially in wild soybean. through the microarray analysis, gssrk was identified as an alkaline (nahco3)-responsive gene, and was subsequently isolated from glycine soja by homologous cloning. gssrk encodes a 93.22kda protein with a highly conserved serine/threonine protein kinase catalytic domain, a g-type l ... | 2013 | 23276523 |
| diversifying selection on flavanone 3-hydroxylase and isoflavone synthase genes in cultivated soybean and its wild progenitors. | soybean isoflavone synthase (ifs) and flavanone 3-hydroxylase (f3h) are two key enzymes catalyzing the biosynthesis of isoflavonoids and flavonoids, both of which play diverse roles in stress responses. however, little is known about the evolutionary pattern of these genes in cultivated soybean and its wild progenitors. herein, we investigated the nucleotide polymorphisms in isoflavone synthase (ifs1, ifs2) and flavanone 3-hydroxylase (f3h2) genes from 33 soybean accessions, including 17 cultiva ... | 2013 | 23342093 |
| genomic differences between cultivated soybean, g. max and its wild relative g. soja. | glycine max is an economically important crop and many different varieties of soybean exist around the world. the first draft sequences and gene models of g. max (domesticated soybean) as well as g. soja (wild soybean), both became available in 2010. this opened the door for comprehensive comparative genomics studies between the two varieties. | 2013 | 23368680 |
| development and evaluation of soysnp50k, a high-density genotyping array for soybean. | the objective of this research was to identify single nucleotide polymorphisms (snps) and to develop an illumina infinium beadchip that contained over 50,000 snps from soybean (glycine max l. merr.). a total of 498,921,777 reads 35-45 bp in length were obtained from dna sequence analysis of reduced representation libraries from several soybean accessions which included six cultivated and two wild soybean (g. soja sieb. et zucc.) genotypes. these reads were mapped to the soybean whole genome sequ ... | 2013 | 23372807 |
| allele-specific marker development and selection efficiencies for both flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase genes in soybean subgenus soja. | color is one of the phenotypic markers mostly used to study soybean (glycine max l. merr.) genetic, molecular and biochemical processes. two p450-dependent mono-oxygenases, flavonoid 3'-hydroxylase (f3'h; ec1.14.3.21) and flavonoid 3',5'-hydroxylase (f3'5'h, ec1.14.13.88), both catalyzing the hydroxylation of the b-ring in flavonoids, play an important role in coloration. previous studies showed that the t locus was a gene encoding f3'h and the w1 locus co-segregated with a gene encoding f3'5'h ... | 2013 | 23463490 |
| a glycine soja aba-responsive receptor-like cytoplasmic kinase, gsrlck, positively controls plant tolerance to salt and drought stresses. | receptor such as protein kinases are proposed to work as sensors to initiate signaling cascades in higher plants. however, little is known about the precise functions of receptor such as protein kinases in abiotic stress response in plants, especially in wild soybean. here, we focused on characterization of the biological functions of a receptor-like cytoplasmic serine/threonine protein kinase gene, gsrlck, which was previously identified as a putative salt-alkali stress-related gene from the tr ... | 2013 | 23494614 |
| [identification of calcium/calmodulin-binding receptor-like kinase gscbrlk-interactive proteins using yeast two-hybrid system]. | gscbrlk (calcium/calmodulin-binding receptor-like kinase from glycine soja) links aba (abscisic acid)- and salt-induced calcium/calmodulin signal in plant cells. in order to study the molecular mechanisms of gscblrk, the salt-treated glycine soja cdna library was screened with pbt3-ste-cbrlk as bait plasmid using yeast two hybrid system. two positive clones (snare and 14-3-3 protein) were identified by constructing cdna library of wild soybean under salt treatment, membrane system yeast two hybr ... | 2013 | 23575546 |
| expression of wild soybean wrky20 in arabidopsis enhances drought tolerance and regulates aba signalling. | the wrky-type transcription factors are involved in plant development and stress responses, but how the regulation of stress tolerance is related to plant development is largely unknown. gswrky20 was initially identified as a stress response gene using large-scale glycine soja microarrays. quantitative reverse transcription-pcr (qrt-pcr) showed that the expression of this gene was induced by abscisic acid (aba), salt, cold, and drought. overexpression of gswrky20 in arabidopsis resulted in a dec ... | 2013 | 23606412 |
| a platform for soybean molecular breeding: the utilization of core collections for food security. | soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. china has the largest collection of cultivated soybeans (glycine max) and wild soybeans (glycine soja) all over the world. the platform of soybean core, mini core and integrated applied core collections has been developed in the past decade based on systematic researches which included the sampling strategies, statistical methods, phenotypic data and ssr markers. me ... | 2013 | 23708950 |