Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| laboratory mating behavior of the plum curculio. | 1969 | 5778308 | |
| the method of pollination with a pollen mixture to obtain interspecific hybrids of plum and cherry. | 1965 | 5883035 | |
| immunogenic variation among the so-called lc strains of mycoplasma mycoides subspecies mycoides. | much evidence of immunogenic heterogeneity among the lc strains of mycoplasma mycoides ssp. mycoides emerged from cross-immunization and -hyper-immunization experiments in mice in which three lc strains (vom/plum island, 74/2488, and mankefår 2833) were used for challenge purposes. all heterologous lc-strain vaccines cross-immunized against the three challenge strains, but protection was usually only 'partial', i.e. significantly less than that given by homologous vaccine. cross-hyperimmunizatio ... | 1983 | 6190898 |
| rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs. | virus particles morphologically similar to caliciviruses and rotaviruses were detected by electron microscopy (em) in the intestinal contents of a 27-day-old diarrheic nursing pig. a third small spherical 23-nm virus-like particle was also observed. calicivirus-like particles averaged 33 nm in diameter. similar to rotaviruses, rotavirus-like particles were present as single-capsid 55-nm forms or double-capsid 70-nm particles. most gnotobiotic pigs orally exposed to samples containing these three ... | 1980 | 6252238 |
| [classifications and therapeutic indications in spontaneous intracerebral hematomas in the absence of any vascular malformation. preliminary study apropos of 65 cases]. | ct scan and i.c.p. monitoring help us in management of spontaneous intracerebral haemorrhage to decide if surgical evacuation is necessary or not. the various opinions expressed in literature seem to depend on the lack of correlations between clinical state, ct scan and i.c.p. monitoring. three groups of patients were studied: group i: patients with ingravescent coma during the first three days. their natural history is death. group 2: patients with disturbances of consciousness which appear, pe ... | 1984 | 6462319 |
| plum-blossom needle therapy in concomitant strabismus. a report of 103 cases. | 1984 | 6565887 | |
| observation on 52 cases of paralysis of common peroneal nerve treated by acupuncture and plum-blossom needling. | 1984 | 6567734 | |
| neurodermatitis treated by plum-blossom needle. | 1984 | 6570497 | |
| nutrient composition of stone fruit (prunus spp.) cultivars: apricot, cherry, nectarine, peach and plum. | 1983 | 6664077 | |
| [a gouty tophus in the temporomandibular joint and on the eustachian tube]. | a 76-year old female patient was suffering from a right-sided conduction-type hearing loss due to a very painful tumour in the anterior parotid region. x-ray films showed a partial destruction of the right temporomandibular joint. since the patient had undergone a left-sided mastectomy for breast carcinoma two years previously, an operation was performed under the suspicion of a metastatic lesion. a plum-sized gouty tophus was found adjacent to the temporomandibular joint. the head of the mandib ... | 1983 | 6672499 |
| the estimation of epistasis in components of fitness in experimental populations of drosophila melanogaster i. a two-stage maximum likelihood model. | laboratory populations of drosophila melanogaster bearing the curly and plum marked second chromosome inversions were observed in selection experiments for ten discrete generations. maximum likelihood estimates of the relative fitnesses of curly, plum, curly-plum, and wild phenotypes were obtained from selection trajectories. using these estimates, measures of multiplicative and additive epistasis were calculated. these were partitioned into pre-sampling and post-sampling components, and both we ... | 1981 | 6792162 |
| the estimation of epistasis in components of fitness in experimental populations of drosophila melanogaster ii. assessment of meiotic drive, viability, fecundity and sexual selection. | an analysis of fitness components was performed on laboratory populations of drosophila melanogaster bearing curly and plum marked second chromosome inversions with two genetic backgrounds. selective differences between curly, plum, curly-plum, and wild phenotypes were partitioned into meiotic drive, viability, fecundity and mating success. although no significant meiotic drive was detected, there were significant differences between genotypes in the other three components. interlocus interactio ... | 1981 | 6792163 |
| [4 simultaneous cases of methanol poisoning caused by home-made plum brandy]. | 1983 | 6845480 | |
| regional cerebral glucose utilization measured with the 2--[ 14c] deoxyglucose technique: its use in mapping functional activity in the nervous system. | the results of studies with the [ 14c] deoxyglucose technique unequivocally establish that functional activity in specific components of the central nervous system is, as in other tissues, closely coupled to the local rate of energy metabolism. stimulation of functional activity increases the local rate of glucose utilization; reduced functional activity depresses it. these changes are so profound that they can be visualized directly in autoradiographic representations of local tissue concentrat ... | 1980 | 6945030 |
| intestinal metaplasia in japan: association with diet. | of 1,749 volunteers in a screening program for gastric cancer in japan, 206 men and 181 women received a systematic gastroscopic examination and responded to a dietary questionnaire estimating their quantitative intake of 33 food items during the past weeks. when diet was correlated with intestinal metaplasia (a precursor lesion of gastric carcinoma) found in gastroscopic biopsies, it was observed that more dried fish consumption and less vitamin a intake increased the extent of intestinal metap ... | 1982 | 6950167 |
| some considerations on the central regulation of respiration and its disturbances. | the effect of increased intracranial pressure on the respiration pattern was studied in cats. it appeared that the different patterns described by plum and brown blend into each other under certain conditions. | 1982 | 7072554 |
| [forme fruste of progressive lipodystrophy or multiple symmetrical surface lipomatosis?]. | it is reported on a 44-year-old patient who has a peculiar form of the disturbance of the distribution of fatty tissue. apart from clear superficial hypertrophies at upper arms, shoulders, thorax and hypogastrium as well as plum-sized nodes at the left femor and retroauricularly discrete atrophies are found in the face, at forearms and lower legs. in very inconspicuous paraclinical findings special forms of the progressive lipodystrophy and of the indolent symmetrical lipomatosis are discussed. | 1982 | 7080563 |
| detection of adulteration in blackberry juice concentrates and wines. | adulteration of blackberry juice concentrates and wines with juice of sorbitol-containing fruits was detected by determining carbohydrates by high performance liquid chromatography, gas-liquid chromatography, and thin layer chromatography. sorbitol is not fermented by yeast and can be detected in wines made from blackberry juice concentrates that contain sorbitol. high levels of sorbitol and quinic and malic acids suggest that an imported blackberry concentrate may have been adulterated with plu ... | 1982 | 7174584 |
| [survey on antibodies against human influenza viruses in the sera of "plum-flower" deer]. | 1982 | 7185425 | |
| free sugars and sorbitol in fruits--a complication from the literature. | the glucose, fructose, sucrose, and sorbitol content of apple, pear, plum, cherry, grape, strawberry, raspberry, blackberry, and peak fruit was compiled from the literature; their range, mean, standard deviation, and percent coefficient of variance were calculated. the individual fruits have characteristic patterns relating to their sorbitol content, glucose: fructose ratio, and sucrose content which are influenced to only a small degree by variety, season, or geographic origin. processing in ma ... | 1981 | 7204315 |
| [two cases of intraventricular meningiomas in children (author's transl)]. | for several months a 14-year-old girl had suffered from increased intracranial pressure which in spite of extensive calcification in the fourth ventricle had not been recognized. during the operation a plum-sized tumour was found free moving in the fourth ventricle. being hard as bone it had to be extracted in toto. the histological examination showed a highly calcified endotheliomatous meningioma. recovery without complications. for seven years a 12-year-old boy had a tumour in the third ventri ... | 1981 | 7343847 |
| [clinical aspects of the prognosis of skull and brain injuries. a study of 108 cases]. | an assessment is made of the extent to which age and the degree an seriousness of the initial neurological picture influenced prognosis in a series of 108 subjects with brain injuries treated in the siena hospital intensive care unit between 1974 and 1977. neurological disease was classified according to posner and plum. mortality was 46.2%. in most cases, age tended to exacerbate the neurological syndrome from the outset. it also facilitated the appearance of respiratory sepsis, which aggravate ... | 1980 | 7465086 |
| properties of the active plum pox potyvirus rna polymerase complex in defined glycerol gradient fractions. | as a first step in the study of the replication of plum pox virus (ppv) rna, an in vitro virus-specific rna polymerase activity was characterized in a crude membrane extract (martin and garcia, 1991). in this study, we report the fractionation of the crude membrane extract by centrifugation in glycerol gradients. the sedimentation properties after different treatments of the crude extract and its insensitivity to micrococcal nuclease treatment suggest that the rna polymerase activity was localiz ... | 1995 | 7483826 |
| production and characterization of monoclonal antibodies to plum pox virus and their use in differentiation of mediterranean isolates. | monoclonal antibodies (mabs) specific to plum pox virus (ppv) were prepared by fusing myeloma cell lines to spleen cells of mice immunized with purified virus, including virus prepared with protease inhibitors to preserve the integrity of the coat protein (cp). the characterized mabs could be used in elisa to differentiate several mediterranean ppv isolates differing in their geographical origin and cp size. at least seven antigenic sites could be established based on the recognition pattern and ... | 1994 | 7526822 |
| rna helicase activity of the plum pox potyvirus ci protein expressed in escherichia coli. mapping of an rna binding domain. | the plum pox potyvirus (ppv) cylindrical inclusion (ci) protein fused to the maltose binding protein (mbp) has been synthesized in escherichia coli and purified by affinity chromatography in amylose resin. in the absence of any other viral factors, the fusion product had ntpase, rna binding and rna helicase activities. these in vitro activities were not affected by removal of the last 103 amino acids of the ci protein. however, other deletions in the c-terminal part of the protein, although leav ... | 1995 | 7538661 |
| transmission by aphids of a naturally non-transmissible plum pox virus isolate with the aid of potato virus y helper component. | two spanish plum pox virus (ppv) isolates, 5.15 and 3.3, were used in transmission experiments involving the aphid vector myzus persicae, with woody and herbaceous host plants. these isolates differ in the size of their coat protein (cp) and sequence analysis revealed that isolate 3.3 has a 15 amino acid deletion near the n terminus of the cp, affecting the same positions as in a previously reported non-aphid-transmissible ppv isolate from germany. aphid transmission experiments showed that isol ... | 1995 | 7561767 |
| genetic relationships among strains of xylella fastidiosa from rapd-pcr data. | genetic relationships among 11 xylella fastidiosa strains isolated from mulberry, almond, ragweed, grape, plum, elm, and citrus were determined by random amplified polymorphic dna (rapd). twenty-two 10-base primers amplified a total of 77 discrete polymorphic bands. phenetic analysis based on a similarity matrix corresponded well with previous reports on x. fastidiosa rflp-based similarity relationships, indicating that rapd-pcr amplification products can be used as a reliable indicator of genet ... | 1995 | 7606188 |
| randomly amplified polymorphic dna analysis of xylella fastidiosa pierce's disease and oak leaf scorch pathotypes. | randomly amplified polymorphic dna analysis was conducted with 14 primers to 17 strains of xylella fastidiosa. there was a high degree of similarity among the seven pierce's disease (pd) strains (sxy > 0.93) and the seven oak leaf scorch (ols) strains (sxy > 0.96). however, the two groups were different, with a similarity index of 0.67, confirming the presence of a pd dna cluster and suggesting the presence of a new ols cluster. the control plum leaf scald strains (two strains) together with the ... | 1995 | 7646005 |
| expression of the plum pox coat protein gene in transgenic nicotiana tabacum plants. | plant expression vector pbi 121 containing the gene encoding coat protein of plum pox virus of the skierniewice isolate (cp ppv-s) was prepared (clone pcm1). the construct was used for transformation of nicotiana tabacum plants using an agrobacterium tumefaciens based system. about 82% of kanamycin resistant plant lines contained a transgene (the sequence of cp ppv-s) but only 81% of them actively expressed the ppv-s coat protein gene as measured by rt-pcr. | 1995 | 7653168 |
| [long-term survival following excision of a neck carotid body tumor]. | farmer with a jugular tumor on the right side and suffering from head and shoulder aches, and epileptic seizures during sleep was admitted to a district hospital and underwent surgery. partial anesthesia was applied and neck vascular area exposed. intraoperatively a tumor of plum size was seen which constricted carotid artery bifurcation with its branches. the pulse in the internal carotid artery was impalpable. carotid artery was clamped, and conversation with the patient begun to find out whet ... | 1994 | 7708559 |
| sensitive non-radioactive nucleic acid hybridization assay for plum pox virus detection. | a new non-radioactive sandwich hybridization assay was designed to simplify the analysis of a large number of plant samples. plant material was homogenized in 0.5% sds and added directly to the hybridization reaction, in which a pair of identifying probes were used. one of the probes was biotinylated capture rna specific for plum pox virus (ppv) strain sk-68; the other rna probe was synthesized from a plasmid bearing the adjacent sequence of this strain and was labelled with digoxigenin (dig). b ... | 1994 | 7709075 |
| [occurrence of aluminum in some foodstuffs]. | results of aluminium determinations in breads and biscuits as well as in fish preserves, vegetables, strawberries and teas including fruit-herb teas are presented. also the percent of extraction of aluminium of various kind of tea leaves into the infusions was determined. the commercially available sample of foods was used for investigation. aluminium was determined by using two methods: a spectrophotometric extraction method with-hydroxyquinoline and less sensitive aas method with nitrous oxide ... | 1994 | 7777774 |
| development and preliminary testing of the neurological assessment instrument. | the purpose of this study was to test a neurological assessment instrument (nai), used to assess neurological function in adults with acute brain lesions. instrument items were designed to reflect different aspects of neurological function and assess consciousness along the arousal/awareness continuum as described by plum and posner. content of the nai was validated by 10 neuroscience expert physicians, nurses and speech pathologists. reliability was tested with one pair of raters on 39 neurolog ... | 1994 | 7829918 |
| simple and rapid preparation of infected plant tissue extracts for pcr amplification of virus, viroid, and mlo nucleic acids. | a rapid, simple method for preparing plant tissues infected with viruses, viroids, or mlos using a commercial product known as gene releaser is described. the gene releaser polymeric matrix method produced plant extracts suitable for pcr amplification without the use of organic solvents, ethanol precipitation, or additional nucleic acid purification techniques. modification of maceration methods and/or extraction buffers resulted in the pcr amplification of potato spindle tuber, apple scar skin, ... | 1994 | 7868647 |
| nucleotide sequence of the coat protein gene of the skierniewice isolate of plum pox virus (ppv). | the coat protein (cp) gene of the skierniewice isolate of plum pox virus (ppv-s) has been amplified using the reverse transcription--polymerase chain reaction (rt-pcr), cloned and sequenced. the nucleotide sequence of the gene and the deduced amino-acid sequence of ppv-s cp were compared with those of other ppv strains. the nucleotide sequence showed very high homology to most of the published sequences. the motif: asp-ala-gly (dag), important for the aphid transmissibility, was present in the a ... | 1994 | 7913278 |
| allergenic cross-reactivity among peach, apricot, plum, and cherry in patients with oral allergy syndrome: an in vivo and in vitro study. | oral allergy syndrome in response to fruits and vegetables frequently occurs as clusters of hypersensitivity to members of the same botanical family, for which the immunologic basis lies in a number of common allergens, most of them still unidentified. | 1994 | 7930303 |
| nectar feeding by the early-spring mosquito aedes provocans. | nectar feeding by males and females of the mosquito aedes provocans was studied at a site near belleville, ontario, canada. canada plum, prunus nigra, and especially pin cherry, p. pensylvanica, bloomed contemporaneously with the emergence of ae. provocans and were important nectar sources for adult mosquitoes during their first week of life. blossoms of p. pensylvanica shielded for 24 h from foragers produced an average of 0.14 mg of sugar (approximately 2.3j). this nectar was avidly sought by ... | 1994 | 7949310 |
| fibre constituents of some foods. | some plant foods viz. bottlegourd, carrot, cauliflower, cabbage, green bengalgram, pea, apple, plum, guava, karonda, blackgram husk and lentil husk were analysed for their dietary fibre components. the total dietary fibre contents of these foods varied from 14.68 to 78.21 percent on dry matter basis. as compared to fruits and vegetables, the husks had higher amount of total dietary fibre. cellulose represented as the major fibre constituent in most of the foods whereas, husks were observed to be ... | 1994 | 7971775 |
| parkinson and the plum tree. | 1994 | 7986170 | |
| effect on lipopolysaccharide structure of aeration during growth of a plum isolate of pseudomonas syringae pv. morsprunorum. | the composition of lipopolysaccharide (lps) extracted with aqueous phenol from a virulent english plum isolate of pseudomonas syringae pv. morsprunorum varied according to the partial pressure of oxygen (po2) in the culture medium at the time of harvest. when po2 was low, the organism grew slowly and produced smooth lps bearing rhamnan sidechains. as po2 was raised, the rate of growth increased and smooth lps was replaced by a rough species deficient in rhamnose, which co-extracted with a d-gluc ... | 1994 | 8075800 |
| comparative sequence analysis of four complete primary structures of plum pox virus strains. | the complete nucleotide sequence of plum pox virus (ppv) strain sk 68 was determined from a series of overlapping cdna clones. the exact 5' terminus was determined by direct rna sequencing. the rna sequence was 9786 nucleotides in length, excluding a 3' terminal poly(a) sequence. the large open reading frame starts at nucleotide position 147 and is terminated at position 9568. comparison of cistrons from other plum pox virus strains with those predicted for the sk 68 strain indicated the same ge ... | 1993 | 8122394 |
| survey of mycoflora and mycotoxins of some dried fruits in egypt. | fifty-five species and two varieties appertaing to 23 genera were collected and identified from 4 samples of dried fig and 3 dried samples of each of apricot, plum and raisin. forty-nine species and two varieties belonging to 20 genera were isolated on 1% glucose-czapek's while 31 species and one variety belonging to 16 genera were isolated on 40% sucrose-czapek's agar medium at 28 +/- 2 degrees c. penicillium, aspergillus and cladosporium were the common genera on the two types of media used. a ... | 1993 | 8229671 |
| treatment of facial spasm by plum-blossom needle tapping and cupping therapy. | 1993 | 8246597 | |
| care allegedly provided without proper supervision. case in point: raicevich v. plum creek medical p.c. 918 f. supp. 2d 929--co (1993). | 1993 | 8265797 | |
| monoclonal antibodies suitable for plum pox virus determination. | monoclonal antibodies (mabs) to plum pox virus (ppv) were obtained after immunization of balb/c mice with purified ppv-w isolate. spleen cells from a mouse showing a high serum titer were used for fusion with sp2/0-ag14 myeloma cells. culture supernatants were screened for specific antibody production against ppv-w isolate using indirect elisa. a total of six stable hybridoma lines producing mabs of igg class were obtained. all four ppv isolates tested (w, a, d and m) can be distinguished by the ... | 1993 | 8314598 |
| systemic phaeohyphomycosis caused by exophiala dermatitidis. | we report a case of systemic phaeohyphomycosis due to exophiala dermatitidis in a 24-year-old man. at the age of 17, the patient had noticed cervical swellings. on palpation at his initial examination, more than a dozen firm lymph nodes between 1 and 5 cm in diameter were found on both sides of the neck and in both axillae. examination of biopsy specimens of lymph nodes revealed pale brown hyphae in granulomatous lesions and exophiala dermatitidis was isolated from mycological cultures of the lo ... | 1993 | 8316255 |
| inhibitory effects of human cystatin c on plum pox potyvirus proteases. | the effect of different protease inhibitors on the proteolytic processing of the plum pox potyvirus (ppv) polyprotein has been analyzed. human cystatin c, an inhibitor of cysteine proteases, interfered with the autoprocessing of the viral papain-like cysteine protease hcpro. unexpectedly, it also had an inhibitory effect on the autocatalytic cleavage of the nla protease which, although it has a cys residue in its active center, has been described as structurally related to serine proteases. othe ... | 1993 | 8343605 |
| complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus. | the complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus (aclsva) was determined. the genome is 7552 nucleotides excluding the poly(a) tail and contains three open reading frames (orfs 1, 2 and 3), encoding proteins with m(r) values of 216,503 (216.5k), 50,453 (50.4k) and 21,394 (21.4k), respectively. nucleotide sequence comparisons between aclsv-a and the previously sequenced aclsv from plum (aclsv-p) showed that the sequence identity at the nucleoti ... | 1993 | 8376968 |
| 3'-terminal sequence of the plum pox virus ps and ŏ6 isolates: evidence for rna recombination within the potyvirus group. | the sequence of the 3'-terminal 1768 nucleotides of the ps and ŏ6 isolates of plum pox virus (ppv) has been determined and compared with that of the equivalent regions of other ppv isolates sequenced previously. the sequenced region is part of the ppv open reading frame encoding the last 186 amino acids of the nib protein and the coat protein (cp, 330 amino acids), followed by a non-coding region of 220 nucleotides and a poly(a) tail. ppv-ps and ppv(-)ŏ6, just like ppv-el amar, show rather high ... | 1993 | 8445362 |
| immunodetection of the plum pox virus helper component in infected plants and expression of its gene in transgenic plants. | tobacco plants (nicotiana tabacum cv. xanthi) have been transformed via agrobacterium tumefaciens vectors, with cdnas corresponding to the plum pox virus (ppv) cistron 2 encoding helper component (hc-pro) and with the first two and half cistrons of the ppv genome. presence of the hc-pro in ppv-infected plants and transgenic plants transformed with the gene coding for this protein was investigated using specific polyclonal antibodies produced against the ppv hc-pro. the results suggest that two p ... | 1993 | 8517789 |
| berenil binding to higher ordered nucleic acid structures: complexation with a dna and rna triple helix. | berenil is an antitrypanosomal agent that binds to nucleic acid duplexes. recently, we reported that this drug can bind to both dna and rna duplexes, while exhibiting properties characteristic of both intercalation and groove binding [pilch, d. s., kirolos, m. a., liu, x., plum, g. e., & breslauer, k. j. (1995) biochemistry 34, 9962-9976]. in this work, we use spectroscopic, calorimetric, and hydrodynamic techniques to demonstrate that berenil also can bind to dna and rna triplexes. our results ... | 1995 | 8519768 |
| localization of fruit tree viruses by immuno-tissue printing in infected shoots of malus sp. and prunus sp. | immuno-tissue printing protocols for the localization of apple chlorotic leaf spot virus (aclsv), stem grooving virus (sgv) and plum pox virus (ppv) in shoots of prunus and malus in vitro have been established for routine diagnosis in a virus elimination program. since these viruses belong to different virus genera, the protocols were adapted according to the properties of the virus under investigation. accumulation of aclsv was highest in the base of the stem and decreased towards the apex of t ... | 1995 | 8537455 |
| effects of land use on the degradability of dissolved organic matter in three watersheds of the plum island sound estuary. | 1995 | 8541418 | |
| print-capture pcr: a simple and highly sensitive method for the detection of plum pox virus (ppv) in plant tissues. | 1996 | 8668554 | |
| a modified device for long term sampling of parotid saliva in various experimental conditions. | a parotid cup has been widely used as an intraoral device for the collection of parotid saliva. however, in long duration sampling, the cup tends to disconnect under circumstances such as mastication, sleep or physical exercise. we have designed a modified device of parotid cup for saliva collection to overcome these unfavorable conditions. the parotid cup was modified by fixing it to the bite-plate appliance which was adjusted individually to the occlusal plane in order to avoid disconnection o ... | 1995 | 8689745 |
| the rna helicase ci from plum pox potyvirus has two regions involved in binding to rna. | the plum pox virus (ppv) protein ci is an rna helicase, whose function in the virus replication is still unknown. recently, an rna binding domain was mapped to a region of the ci protein that includes the arginine-rich motif vi typical of rna helicases of the superfamily sf2. in the present study, a second region involved in rna binding activity of the ci protein has been identified. northwestern assays with a series of maltose-binding protein fusions that contain different ci fragments showed t ... | 1996 | 8690088 |
| [polyostotic hyperostosis in a plum-headed parakeet (psittacula cyanocephala l., 1766]. | with emphasis on a case report, the clinical and pathological findings commonly associated with polyostotic hyperostosis as well as its etiology are discussed, and the available literature is reviewed. the diagnostic specifics of the disease are detailed, and a survey of possible differential diagnoses is given. | 1996 | 8720953 |
| cold calcium signaling in arabidopsis involves two cellular pools and a change in calcium signature after acclimation. | cold shock elicits an immediate rise in cytosolic free calcium concentration ([ca2+]cyt) in both chilling-resistant arabidopsis and chilling-sensitive tobacco (nicotiana plumbaginifolia). in arabidopsis, lanthanum or egta caused a partial inhibition of both cold shock [ca2+]cyt elevation and cold-dependent kin1 gene expression. this suggested that calcium influx plays a major role in the cold shock [ca2+]cyt response and that an intracellular calcium source also might be involved. to investigate ... | 1996 | 8721751 |
| frequent occurrence of recombinant potyvirus isolates. | we have performed a systematic search for recombination in the region encoding coat protein and the 3' non-translated region in natural isolates of potyviruses, the largest group of plant rna viruses. the presence of recombination, and the localization of the cross-over points, were confirmed statistically, by three different methods. recombination was detected or suspected in 18 out of 109 potyvirus isolates tested, belonging to four out of eight virus species, and was most prevalent in potato ... | 1996 | 8760448 |
| unusual foreign bodies of upper gastrointestinal tract. | we report management of unusual foreign bodies of upper gastrointestinal tract, namely beer bottle cap, raisins and pistachu, mango peel, betelnut and plum seed at a university hospital in northern india. | 1995 | 8854958 |
| first findings of plum pox virus in walnut trees (juglans regia l.) | plum pox virus (ppv) was transmitted from infected buds and leaves of walnut tree (juglans regia l.) on the following herbaceous indicators: chenopodium foetidum schrad., nicotiana bigelovii var. quadrivalvis fuchs., n. clevelandii x n. glutinosa. a positive elisa reaction with antisera against ppv was obtained from infected buds and leaves of juglans regia l. and from attacked leaves of indicator plants. | 1996 | 8886101 |
| epidemiologic association between antibody titres against bovine virus diarrhoea virus, rinderpest disease virus and infectious bovine rhinotracheitis virus in a buffalo herd. | the association between antibody titres against bovine virus diarrhoea virus (bvdv), infectious bovine rhinotracheitis virus (ibrv) and rinderpest disease virus (rpdv) in buffaloes was investigated in a cross-sectional study. thirty-six lactating buffaloes from a herd in landhi cattle colony, karachi were bled and serum samples subjected to a micro-neutralisation test at the plum island animal disease center, usa, to categorise each buffalo either as positive or negative against each of 3 viral ... | 1996 | 8888525 |
| a nurse with anaphylaxis to stone fruits and latex sensitivity: potential diagnostic difficulties to consider. | there have been several recent reports describing a cross-reactivity between latex and antigens derived from a number of fruits, including those of the stone fruit family. the relationships between the allergic reactions to the antigens from these plant products are still being defined. | 1996 | 8970444 |
| physico-chemical and sensory properties of formulated syrup from black plum (vitex doniana) fruit. | the fresh ripe fruit pulp and syrup, produced using the open pan evaporator, of vitex doniana were analysed physico-chemically. results show that the edible pulp of the ripe fruit is fairly rich in vitamin c (18.1 mg/100 ml) but acidic (ph 4.38) and poor in protein (0.82%) and oil (0.56%). the syrup yielded a de value of 52, total dissolved solids content of 67 degrees brix and a refractive index of 1.4762 at 20 degrees c. the syrup was moderately viscous (2.1 poiseuille), acid (ph 4.45) but rel ... | 1996 | 8983056 |
| agenesis of the corpus callosum associated with paroxysmal hypothermia: shapiro's syndrome. | spontaneous recurrent hypothermia and hyperhidrosis associated with agenesis of the corpus callosum was first described by shapiro and plum in 1967. since then, several cases with similar symptoms (now known as shapiro's syndrome or spontaneous periodic hypothermia) have been described. we report another case of this syndrome in a 21-year-old-man, and discuss possible pathogenetic mechanisms and therapeutic approaches. | 1997 | 9038041 |
| processing of the plum pox virus polyprotein at the p3-6k1 junction is not required for virus viability. | proteolytic processing of the potyvirus polyprotein is mainly performed by the virus-encoded nia protease, whose cleavage sites are characterized by conserved heptapeptide sequences. partial processing at the cleavage site present between the p3 and 6k1 cistrons by the plum pox potyvirus (ppv) nia protease has been previously shown to occur in vitro. we have now studied the role of polyprotein processing at the p3-6k1 junction in vivo, using a full-length ppv cdna clone. ppv mutant transcripts c ... | 1995 | 9049341 |
| analysis of the sequence diversity of the p1, hc, p3, nib and cp genomic regions of several yam mosaic potyvirus isolates: implications for the intraspecies molecular diversity of potyviruses. | partial sequences from serologically characterized yam mosaic potyvirus (ymv) isolates were determined in conserved (helper-component proteinase, hc; nuclear inclusion b, nib) and variable (first protein, p1; third protein, p3; and coat protein, cp) regions of the potyviral genome in order to investigate the intraspecies molecular diversity of ymv. multiple sequence alignments and pairwise comparisons were used to quantify the sequence polymorphism in these regions. two levels of diversity were ... | 1997 | 9191916 |
| long sequences in the 5' noncoding region of plum pox virus are not necessary for viral infectivity but contribute to viral competitiveness and pathogenesis. | the 5'-terminal 31 nucleotides of the 146-nucleotides-long 5' noncoding region of plum pox potyvirus (ppv) are highly conserved in all the members of the potyvirus genus. to map the sequences of the 5' noncoding region that are necessary in vivo for infectivity, we have constructed a nested set of substitution and deletion mutants. while we were not able to infect nicotiana clevelandii plants with full-length ppv transcripts bearing mutations in the 5'-terminal 35 nucleotides of the viral genome ... | 1997 | 9201225 |
| fungi associated with post-harvest rot of black plum (vitex doniana) in nigeria. | the fungi associated with rot of vitex doniana fruits (blackplum) were isolated and identified. aspergillus niger, botryodiplodia theobromae, candida spp. penicillium chrysogenum, rhizopus stolonifer, fusarium pallidoroseum f. oxysporum and mucor mucedo were the primary rot causing fungi in contrast to cladosporium herbarum and mucor circinelloides which were just present as secondary colonizers. the rot fungi penetrated mainly through wounds and bruises on the surface of fruits. mature green fr ... | 1996 | 9208478 |
| serological characterization of hungarian plum pox virus isolates. | three hungarian (no. 2, 4 and 9), and a moldavian (k) plum pox virus isolates were compared with a characterized spanish isolate (5.15) by rt-pcr, elisa, dot-blot and western blot analysis. monoclonal antibodies prepared against the external, intermediate and internal sequences of the coat protein of the spanish isolate were able to differentiate the four isolates. hungarian isolate no. 2 proved to be serologically identical to the spanish isolate, while no. 4 showed appreciable differences and ... | 1997 | 9232895 |
| a case-control study of risk factors of nasopharyngeal carcinoma. | risk factors and nasopharyngeal carcinoma (npc) in residents of guangzhou city were investigated in a case-control study: 104 cases were compared with an equal number of age, sex, and neighborhood-matched controls. the results of multiple conditional regression of logistic model showed that plum vegetable (adjusted or = 1.81, 95% ci 1.01-3.33), preserved prune (adjusted or = 2.95, 95% ci 1.04-8.41), no separate kitchen (if >35 years, adjusted or = 1.98, 95% ci 1.24-3.75), kitchen range without c ... | 1997 | 9233826 |
| design of a solubilization pathway for recombinant polypeptides in vivo through processing of a bi-protein with a viral protease. | an artificial maturation pathway for increasing the solubility in vivo of recombinant proteins overproduced in escherichia coli is reported, which is based on the proteolytic processing of viral polyproteins. the gene product of interest is expressed as a fusion to a heterologous moiety (i.e. the maltose binding protein, male) in order to increase the overall solubility of the hybrid. the hinge region between the two fusion partners contains a cleavage site for the nia protein, a very specific p ... | 1997 | 9278287 |
| specific detection of d- and m-isolates of plum pox virus by immunoenzymatic determination of pcr products. | molecular techniques based on the polymerase chain reaction (pcr) can provide rapid and sensitive diagnosis of plum pox virus (ppv), the causal agent of the devastating 'sharka' disease of stone fruit trees. the present study compared routine polymerase chain reaction (pcr) procedures against a new system, pcr-elisa (boehringer mannheim), which enables immunoenzymatic detection of pcr products. the results show that this hybridisation system ensures fast and more sensitive detection of ppv assoc ... | 1997 | 9300377 |
| cap-independent leaky scanning as the mechanism of translation initiation of a plant viral genomic rna. | the genome of plum pox virus contains a single open reading frame that is translated into a large polyprotein. although the open reading frame starts at nucleotide 36 (36aug), it is translated from the second, 147aug, which is in a more favourable context for translation initiation. we have carried out in vitro translation and transient expression analysis in protoplasts of a nested set of substitution and deletion mutants, and the results show that no internal structure in the 5' noncoding regi ... | 1997 | 9349492 |
| cloning, sequencing, and expression of the mig gene of mycobacterium avium, which codes for a secreted macrophage-induced protein. | mycobacterium avium is an intracellular pathogen that has evolved to be a frequent cause of disseminated infection in immunocompromised patients. although these bacilli are readily phagocytized, they are able to survive and even multiply within human macrophages. the process whereby mycobacteria circumvent the lytic functions of the macrophages is currently not well understood, but this is a key aspect in the pathogenicity of all pathogenic mycobacteria. previously, we identified a gene in m. av ... | 1997 | 9353032 |
| the motif v of plum pox potyvirus ci rna helicase is involved in ntp hydrolysis and is essential for virus rna replication. | the plum pox potyvirus (ppv) protein ci is an rna helicase whose function in the viral life cycle is still unknown. the ci protein contains seven conserved sequence motifs typical of rna helicases of the superfamily sf2. we have introduced several individual point mutations into the region coding for motif v of the ppv ci protein and expressed these proteins in escherichia coli as maltose binding protein fusions. mutations that abolished rna helicase activity also disturbed ntp hydrolysis. no mu ... | 1997 | 9358154 |
| simultaneous detection and typing of plum pox potyvirus (ppv) isolates by heminested-pcr and pcr-elisa. | two techniques for simultaneous detection and typing of plum pox potyvirus (ppv) isolates belonging to the d or m serotypes, heminested pcr (h-pcr) and pcr-elisa, have been developed. ten ppv isolates typed using ppv-d and ppv-m specific monoclonal antibodies by elisa-dasi were used to validate these two methods. the results obtained show a complete coincidence of the nucleic acid-based techniques with the serological data. when serial dilutions of infected plant extracts were assayed, h-pcr and ... | 1997 | 9389402 |
| hop stunt viroid (hsvd) sequence variants from prunus species: evidence for recombination between hsvd isolates. | hop stunt viroid (hsvd) is able to infect a number of herbaceous and woody hosts, such as grapevine, citrus or prunus plants. previous phylogenetic analyses have suggested the existence of three major groups of hsvd isolates (plum-type, hop-type and citrus-type). the fact that these groups often contain isolates from only a limited number of isolation hosts prompted the suggestion that group-discriminating sequence variations could, in fact, represent host-specific sequence determinants which ma ... | 1997 | 9400968 |
| plum pox potyvirus resistance associated to transgene silencing that can be stabilized after different number of plant generations. | nicotiana benthamiana plants were transformed with a fragment of the plum pox potyvirus (ppv) genome that encodes the nuclear inclusion a (nia) and b (nib) proteins and the n-terminus of the capsid protein (nia-nib-cp). lines transformed with this ppv genomic fragment harboring mutations in the gdd replicase-motif were also obtained. plants of niadeltav lines that carry a gdd to vdd mutation in the ppv transgene, were immune to ppv infection. the resistance was highly specific, since it was only ... | 1998 | 9469941 |
| production of strain specific antibodies against a synthetic polypeptide corresponding to the n-terminal region of the plum pox potyvirus coat protein. | comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (ppv-swc) with the corresponding regions of several other ppv strains indicated that the main differences are in the n-terminal region. polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of the n-terminal region of ppv-swc coat protein. they specifically detected ppv-swc in different immunochemical tests. | 1997 | 9504763 |
| a sex-linked ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in culex pipiens. | an acetylcholinesterase (ache) gene, ace.x, showing 93% identity of deduced amino acid sequence to anopheles stephensi ace has been cloned from a culex pipiens strain homozygous for insensitive ache (iache) mediated insecticide resistance. dna sequence of genomic dna clones identified exons 2-5. rflp of six clones indicated four possible alleles. linkage analysis located ace.x to chromosome i, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimor ... | 1998 | 9535157 |
| specific oligonucleotide primers for the direct detection of plum pox potyvirus-cherry subgroup. | a specific polymerase chain reaction assay was developed for direct identification of the distinct subgroup of plum pox potyvirus (ppv) isolates from cherry trees (ppv-cherry, ppv-c) and its differentiation from other known subgroups of ppv. the specificity of the assay is based on using a pair of primers whose nucleotide sequences are located on the coat protein gene of ppv-sour cherry (soc) at regions of high nucleotide divergence between ppv-soc and other isolates of ppv. the technique will b ... | 1998 | 9562418 |
| effect of gamma irradiation on the production of cell wall degrading enzymes by aspergillus niger. | fungi occurring in egyptian fruits in the city of qena were studied. results from the examination of 25 replicated samples of plums, pears and apples are reported. examinations were carried out by direct plating after surface disinfection in a 0.5% (w/v) calcium hypochlorite solution on czapek's-dox agar. the dominant fungus found in the three types of fruit was aspergillus niger, which was present in 88% of plum samples, 80% of pear samples and all of the apple samples. the lowest dose of gamma ... | 1998 | 9600619 |
| susceptibility of peach gf 305 seedlings and selected herbaceous plants to plum pox virus isolates from western slovakia. | the susceptibility of peach gf 305 seedlings and herbaceous plants to five plum pox virus (ppv) isolates from orchards of western slovakia was investigated. ppv was isolated from diseased plum, apricot and peach trees, and transmitted by chip-budding to peach gf 305. the herbaceous plants were infected by mechanical inoculation. the transmission was analysed by symptomatology and double sandwich enzyme-linked immunosorbent assay (das-elisa). infected peaches developed leaf distortion, tissue cle ... | 1997 | 9607094 |
| development of an antigen presentation system based on plum pox potyvirus. | the development of an antigen presentation system based on the plum pox potyvirus (ppv) is here described. the amino-terminal part of ppv capsid protein was chosen as the site for expression of foreign antigenic peptides. modifications in this site were engineered to avoid the capability of natural transmission by aphids of this ppv vector. as a first practical attempt, different forms of an antigenic peptide (single and tandem repetition) from the vp2 capsid protein of canine parvovirus (cpv) w ... | 1998 | 9607317 |
| nicotiana benthamiana plants transformed with the plum pox virus helicase gene are resistant to virus infection. | nicotiana benthamiana domin. plants were transformed with the cytoplasmic inclusion protein (ci) gene of plum pox potyvirus (ppv) to investigate, whether this non-structural protein would be able to confer resistance. the ci protein is an rna helicase, which contains a conserved nucleotide binding motif (ntbm) and plays an important role in viral replication. two gene constructions were developed for plant transformation. the first contains the original coding sequence of the ci gene under the c ... | 1998 | 9617773 |
| use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants. | aphid transmission of a non-aphid-transmissible strain of zucchini yellow mosaic virus (zymv-nat) occurs in transgenic plants expressing the plum pox potyvirus (ppv) coat protein (cp) gene. heteroencapsidation has been shown to be responsible for this modification in the epidemiological characteristics of the infecting virus. in order to prevent this biological risk, several modified ppv cp constructs were produced that were designed to interfere with heteroencapsidation itself or to block aphid ... | 1998 | 9634095 |
| frontiers of neurology: a symposium in honor of fred plum. new york city, new york, usa. october 5, 1996. | 1997 | 9634449 | |
| mercury in water and sediments of the southern baltic sea. | total mercury concentrations [hgt] were measured in samples of water and sediments collected from the southern baltic sea from the period 1993-1995. picomolar concentrations of hgt were found in offshore surface water. higher levels were measured in samples from the plum of the vistula. an exceptionally high concentration of mercury (25 pm hgt, 5 pm methylmercury) was measured in the near bottom, quasi-anoxic water of the gdañsk deep and explained by the release of mercury from fine grained sedi ... | 1998 | 9652127 |
| multigeneration exposure test of drosophila melanogaster to elf magnetic fields. | mutations, other than dominant lethals, were accumulated on wild type second chromosomes (+) of drosophila melanogaster during exposure to 50 hz sinusoidal alternating magnetic fields of 0.5 or 5 mt (rms) for 40 generations by the curly/plum(cy/pm) accumulation method. we maintained, for 40 generations under continuous exposure, each (+) chromosome as a heterozygote with (cy) chromosome. viability of the (+) chromosome was tested by sib-mating of (cy/+) male and (cy/+) female in a culture every ... | 1998 | 9738524 |
| strain variability of plum pox virus isolates from western slovakia. | leaf tissues of stone fruit trees (plum, apricot, peach and myrobalan) carrying symptoms of plum pox virus (ppv) infection and of peach gf 305 seedlings and nicotiana benthamiana infected experimentally with ppv were assayed for ppv by polymerase chain reaction (pcr). the expected 243 bp pcr products were subjected to restriction fragment length polymorphism (rflp) analysis with restriction endonucleases alui and rsai. all of the pcr products contained the alui site. the rsai restriction profile ... | 1998 | 9770072 |
| plum stones: an unusual cause of ileostomy obstruction demonstrated by sonography. | bowel obstruction is a common cause of stomal complications of ileostomy. we report a case in which sonography revealed 2 well-defined, rounded, hyperechoic structures, measuring 1.7 cm in diameter and causing dense acoustic shadowing, in the lumen of the bowel of a 3-year-old boy with a permanent end ileostomy. endoscopy showed the structures to be freely mobile plum stones in the fluid-filled terminal ileum. this case demonstrates the usefulness of sonography in the diagnostic workup of patien ... | 1998 | 9783251 |
| properties of a virus causing mosaic and leaf curl disease of celosia argentea l. in nigeria. | a sap transmissible virus, causing mosaic and leaf curl disease of celosia argentea, was isolated at vegetable farms in amuwo odofin, tejuoso, and abule ado, lagos, nigeria. the virus had a restricted host range confined to a few species of the amaranthaceae, chenopodiaceae and solanaceae families. it failed to infect several other species of the aizoaceae, brassicaceae, cucurbitaceae, fabaceae, lamiaceae, malvaceae, poaceae and tiliaceae families. the virus was transmitted in a non-persistent m ... | 1998 | 9842442 |
| ultrastructural localization of nonstructural and coat proteins of 19 potyviruses using antisera to bacterially expressed proteins of plum pox potyvirus. | antisera to the bacterially expressed nonstructural proteins (nsp) hc-pro, ci, nia, and nib and the coat protein (cp) of plum pox potyvirus (ppv) were used for analysing the composition of virus-induced cytoplasmic and nuclear inclusions by electron microscopy. the antisera reacted with nsp and cp of ppv on immunogold-labelled ultrathin sections. antiserum to cp reacted with virions of seven out of 18 other potyviruses. cp was distributed throughout the cytoplasm of infected cells. antisera to p ... | 1998 | 9856098 |
| nucleotide sequence of the putative replicase gene of the sour cherry strain of plum pox potyvirus. | the complete nucleotide sequence of the nib coding region of the sour cherry strain of plum pox potyvirus (ppv-soc) has been determined. it consists of 1554 nucleotides and encodes a putative replicase protein of 518 amino acids. sequence identity scores between nib of ppv-soc and other isolates of ppv are significantly low (c. 78%). many of the nucleotide substitutions, however, are silent. ppv-soc differs from isolates of ppv-d, ppv-m and ppv-e1 amar at multiple amino acid positions that are c ... | 1998 | 9856106 |
| susceptibility to recombination rearrangements of a chimeric plum pox potyvirus genome after insertion of a foreign gene. | infectious rna transcripts were generated from a chimeric cdna clone of the plum pox potyvirus (ppv) genome containing the bacterial beta-glucuronidase (gus) gene inserted between the sequences coding for the p1 and hc proteins. an artificial cleavage site specific for the nia viral proteinase was engineered between the gus and hc sequences to produce free gus and hc proteins. the resulting virus ppvgus/ was stably maintained during the first round of infection, although plants remained symptoml ... | 1998 | 9870586 |
| isolation of hiv-1 rna from plasma: evaluation of seven different methods for extraction (part two). | some new commercial methods for the extraction of viral rna have been introduced recently. in addition to the study published previously (verhofstede, c., reniers, s., van wanzeele. f., plum j., 1996. aids 8, 1421-1427), seven different methods (four newly developed and three reference methods) for extraction of hiv-1 rna from plasma have been evaluated. the rna preparation method that gave the best results (acceptable reproducibility, highest sensitivity, reasonable price, fast and easy to perf ... | 1998 | 9923750 |
| mitotic stability of infection-induced resistance to plum pox potyvirus associated with transgene silencing and dna methylation. | plum pox potyvirus (ppv) infection of transgenic nicotiana benthamiana plants that expressed the ppv nib rna replicase carrying a gly to val mutation at the gdd motif (nibv lines) induced a phenotype of virus resistance and transgene silencing, which was not transmissible to the progeny after self-fertilization (h. s. guo and j. a. garcía, mol. plant-microbe interact. 10:160-170, 1997). here, we demonstrate that the induced resistance of nibv plants is mitotically stable after plant propagation ... | 1999 | 9926412 |
| anoxic and hypoxic immature rat model for measurement of monoamine using in vivo microdialysis. | the immature brain is considered relatively resistant to anoxia and ischemia. although hypoxia without ischemia has not been considered to produce brain damage in immature rats as well as in adult rats (s. levine, anoxic-ischemic encephalopathy in rats, am. j. pathol., 36 (1960) 1-17 [8]; d.e. levy, j.b. brieley, d.g. silverman, f. plum, brief hypoxia-ischemia initially damages cerebral neurons, arch. neurol., 32 (1975) 450-456 [9]; j.e. rice, r.c. vannucci, j.b., brieriey, the influence of imma ... | 1999 | 9974139 |
| pest status of american plum borer (lepidoptera: pyralidae) and fruit tree borer control with synthetic insecticides and entomopathogenic nematodes in new york state. | surveys were conducted in 1994 and 1995 to determine the pest status of the american plum borer, euzophera semifuneralis (walker), in new york state stone fruit crops. these surveys indicate that american plum borer is the most important of the wood-boring insects infesting tart cherries and also is an important pest in peaches suffering from canker diseases. it is not prevalent in plums or in healthy peaches. trials to control american plum borer were conducted in tart cherry and peach by using ... | 1999 | 10036985 |