Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| induction of suppressor cells by mycobacterium paratuberculosis antigen in inflammatory bowel disease. | we studied the m. paratuberculosis-induced proliferation and suppressor cell generation by peripheral blood lymphocytes from patients with inflammatory bowel disease. peripheral blood lymphocytes were separated from 33 patients with crohn's disease, 18 with ulcerative colitis, nine with other intestinal diseases, and five with autoimmune disorders. proliferation of peripheral blood lymphocytes from normal individuals in response to 10 micrograms/ml of m. paratuberculosis antigen was reduced by d ... | 1991 | 1825189 |
| experimental disease in young chickens induced by a mycobacterium paratuberculosis isolate from a patient with crohn's disease. | the susceptibility of young chickens to infection with an isolate of mycobacterium paratuberculosis which had been recovered from diseased tissues of a patient with crohn's disease was determined. two-week-old leghorn-cochin chicks were inoculated with strain linda. six birds received 10(7) organisms orally, six intraperitoneally, and five intracardially. six uninoculated birds served as contact controls. birds from each group were necropsied at two-week intervals. focal granulomatous lesions oc ... | 1991 | 1832080 |
| evaluation of enzyme-linked immunosorbent assay in comparison with complement fixation test for the diagnosis of subclinical paratuberculosis in cattle. | an enzyme-linked immunosorbent assay (elisa) was evaluated and compared in parallel with the standard complement fixation test (cft) for the diagnosis of bovine subclinical paratuberculosis. bovine sera preabsorbed with the mixture of mycobacterium phlei and kaolin suspension were assayed for antibody activities to the crude protoplasmic antigen of mycobacterium paratuberculosis in the elisa. elisa antibody titer was expressed as elisa antibody index (eai) value: eai = (at-an)/(ap-an), where at, ... | 1991 | 1834198 |
| lectinhistochemical study on normal and paratuberculosis-affected bovine ileum. | 1991 | 1834225 | |
| correction of antigen-specific t cell defects in aged murine gut-associated lymphoid tissues an immune intervention by combined adoptive transfer of an antigen-specific immunoregulatory cd4 t cell subset and interleukin 2 administration. | gut-associated lymphoid tissues (galt) from aged mice enterically immunized with mycobacterium paratuberculosis protoplasmic antigen show hyperreactive humoral immune responses; this hyperresponsiveness can be corrected to a considerable extent, but not entirely, by systemic administration of interleukin 2 (il 2) alone. the aim of the present study was to determine further whether the hyperreactivity in the antigen-specific humoral immune responses in aged galt could be fully restored by adoptiv ... | 1991 | 1836186 |
| suppressive effect of nonviable mycobacterium paratuberculosis on the delayed-type hypersensitivity reaction to sheep erythrocytes in mice. | the effect of nonviable mycobacterium paratuberculosis on the delayed-type hypersensitivity reaction to sheep erythrocytes (srbc) in mice was evaluated by means of delayed-type footpad swelling. intraperitoneal (i.p.) injection with nonviable m. paratuberculosis into mice from 28 days before to 1 day after immunization with srbc resulted in a significant suppression of foot-pad swelling to srbc. the suppressive effect could be transferred by i.p. injection of spleen cells or peritoneal exudate c ... | 1991 | 1836513 |
| specific detection of mycobacterium paratuberculosis by dna hybridisation with a fragment of the insertion element is900. | this paper describes the evaluation of a newly developed dna probe for mycobacterium paratuberculosis. dna probe pcr278 is a 278 bp fragment obtained by polymerase chain reaction (pcr) amplification of the 5'-region of is900, an insertion element contained in the genome of m paratuberculosis. this dna probe can specifically distinguish m paratuberculosis from a wide range of other organisms, including members of the m avium-m intracellulare complex. when used in conjunction with the pcr amplific ... | 1991 | 1851124 |
| enzyme-linked immunosorbent assay for detection of antibodies against mycobacterium paratuberculosis in goats. | using a heat and sonicated mycobacterium paratuberculosis cordoba antigen (coa1) and the commercial protoplasmic-antigen (ppa-3) as antigens, an elisa for detecting goat antibodies was standardized. when 2 reference populations, 1 positive (17 goats) and the other negative (63 goats) to disease, were used, this test showed 87.5% sensitivity and 93.6% specificity for coa1, and 88.2 and 95.2%, respectively for ppa-3. absorption with m phlei was performed; no significant differences were found for ... | 1991 | 1883088 |
| development and evaluation of a rapid absorbed enzyme immunoassay test for the diagnosis of johne's disease in cattle. | an absorbed enzyme immunoassay (eia) test for johne's disease in cattle was developed in which absorption of cross-reacting antibodies occurred as a rapid reaction in solution rather than overnight with whole organisms and a subsequent centrifugation step. total test time was reduced to less than 2 h with a minimum of manipulations. the test was evaluated in cattle herds from johne's disease-endemic and johne's disease-free regions of australia. specificity was 99.8%. calculations of sensitivity ... | 1991 | 1883290 |
| complete nucleotide sequence of a gene encoding the 70 kd heat shock protein of mycobacterium paratuberculosis. | 1991 | 1886778 | |
| an epidemiological study of paratuberculosis in dairy cattle in ontario: study design and prevalence estimates. | an observational study involving 304 dairy herds and three abattoirs was conducted between 1986 and 1989 to investigate the epidemiology of paratuberculosis in dairy cattle in ontario. the objectives of this paper were to describe the method of data collection for the study, to present descriptive production statistics and to present estimates of the prevalence of paratuberculosis among dairy cattle in ontario. mycobacterium paratuberculosis was isolated from the distal ileum and/or the ileoceca ... | 1991 | 1889036 |
| an evaluation of selected screening tests for bovine paratuberculosis. | the objective of this study was to evaluate the performance of the lipoarabinomannan antigen enzyme-linked immunosorbent assay (lam-elisa), carbohydrate antigen complement fixation (ch-cft), and protein d antigen agar gel immunodiffusion (d-agid) tests for bovine paratuberculosis, relative to histopathology, and to culture and isolation of mycobacterium paratuberculosis from tissues and feces. samples for test evaluation were collected from four sources including blood and tissues from 400 cull ... | 1991 | 1909601 |
| reactivity of fungal organisms in tissue sections using anti-mycobacteria antibodies. | sixty-four cases of deep fungal infections diagnosed using pas or silver stains and 18 control cases of sarcoidosis, m. tuberculosis and m. leprae infection were stained using commercial polyclonal antibody raised against m. paratuberculosis (mp), m. duvalii (md), and bacillus calmette-guerin (bcg). nine of 13 cases of sporotrichosis stained positively using anti-mp antibody only; 13 of 14 cases of histoplasmosis stained with anti-bcg, anti-md, and anti-mp; seven cases of cryptococcosis had only ... | 1991 | 1918507 |
| serologic enzyme-linked immunosorbent assay responses of calves vaccinated with a killed mycobacterium paratuberculosis vaccine. | the purpose of this study was to document the effect of calfhood vaccination for mycobacterium paratuberculosis on a serologic elisa. fifteen calves vaccinated with a killed paratuberculosis vaccine and 5 unvaccinated control calves were tested from the first through the fifteenth month of life. age of vaccination ranged from 5 to 40 days. blood samples were collected prior to vaccination and periodically thereafter. serum antibody was analyzed by use of the elisa. all calves were elisa-negative ... | 1991 | 1928899 |
| comparison of different media for the isolation of small ruminant strains of mycobacterium paratuberculosis. | two different egg based media, with and without the incorporation of sodium pyruvate, were used to isolate m. paratuberculosis from sheep, goat and cattle samples obtained at our laboratory for two years. both media were adequate for bovine material, with a slightly improved isolation rate for herrold's egg yolk medium incorporating sodium pyruvate; however, most of the small ruminant strains grew only on löwenstein-jensen medium without sodium pyruvate. those results point out the need to use d ... | 1991 | 1949552 |
| spontaneous murine thymocyte comitogenic activity consistent with interleukin-1 in cattle naturally infected with mycobacterium paratuberculosis. | the production of comitogenic activity consistent with interleukin-1 (il-1) activity by blood monocytes from cattle with naturally acquired paratuberculosis was examined by murine thymocyte proliferation. in addition, il-1-like activity in response to homologous and heterologous antigens was determined. activity was determined in nine cattle naturally infected with mycobacterium paratuberculosis and six non-infected cattle. comitogenic properties were measured in response to m. paratuberculosis ... | 1991 | 1949571 |
| comparison of the johne's absorbed eia and the complement-fixation test for the diagnosis of johne's disease in cattle. | a commercially available absorbed elisa for the diagnosis of johne's disease (jd) (paratuberculosis) in cattle, the johne's absorbed eia, was compared with the conventional complement-fixation test (cft) used in australia. stored plasma from 3 victorian dairy herds with a history of jd, sera from specimens submitted from animals showing clinical signs of jd and sera from the us national repository for paratuberculosis specimens were used to determine the sensitivity of each test. the eia detecte ... | 1991 | 1953547 |
| some immune responses in cattle exposed to mycobacterium paratuberculosis after injection with modified-live bovine viral diarrhea virus vaccine. | delayed-type hypersensitivity responses to mycobacterium paratuberculosis purified protein derivative (ppd) were decreased in cows experimentally exposed to m. paratuberculosis 7 days after exposure to a modified-live bovine viral diarrhea virus (ml-bvdv) vaccine. in vitro lymphocyte blastogenic responses to phytohemagglutinin were decreased in each of 3 cows 7 days after exposure to ml-bvdv vaccine. also, decreased lymphocyte blastogenic responses to m. paratuberculosis ppd were observed in cul ... | 1990 | 1965582 |
| characterization of mycobacterium paratuberculosis and organisms of the mycobacterium avium complex by restriction polymorphism of the rrna gene region. | nineteen mycobacterium paratuberculosis strains, including strains of bovine, caprine, ovine, cervid, subhuman primate, and human origins, were compared with organisms of the m. avium complex by restriction fragment length polymorphism with a 5s rrna gene probe as the reference dna. mycobacterial dna was extracted, digested with several restriction enzymes, subjected to electrophoresis and southern blotting, and then hybridized with a 5s rrna gene probe from escherichia coli. hybridizing bands w ... | 1990 | 1969866 |
| identification of restriction fragment length polymorphisms in dna from mycobacterium paratuberculosis. | dnas from 34 mycobactin-dependent isolates of mycobacterium paratuberculosis and 1 isolate of m. paratuberculosis 18 were digested with four restriction endonucleases. southern hybridization experiments were performed with a 32p-labeled oligonucleotide dna probe derived from the sequence of is900, an insertion sequence present in 15 to 20 copies per m. paratuberculosis chromosome. the probe hybridized with dna from each of the mycobactin-dependent isolates, and restriction fragment length polymo ... | 1990 | 1979332 |
| mycobacterium paratuberculosis and crohn's disease. | the possible aetiological role of mycobacterium paratuberculosis in crohn's disease was investigated. the immunological response was studied using an enzyme-linked immunosorbent assay (elisa), western blotting, and immunocytochemistry. the antibody response to two protoplasmic antigen preparations of m paratuberculosis in the sera of patients with inflammatory bowel disease was measured by elisa. igg and igm antibodies to these antigens were measured in serum samples from 52 patients with crohn' ... | 1991 | 1991637 |
| crossed immunoelectrophoretic analysis of mycobacterium paratuberculosis. | antigenic analysis of m. paratuberculosis revealed extensive cross-reactivity with m. avium; however, the number of cross-reactive antigens found was dependent on the strain of m. avium tested. one antigen was shown to be the common antigen while another appeared to be iron-regulated in its production. a commercial polyclonal antibody to m. paratuberculosis produced a cie precipitin pattern comparable to that of the antibody produced for the present study. an antigen designated no. 6 was consist ... | 1991 | 1993120 |
| evaluation of a commercial enzyme-linked immunosorbent assay for johne's disease. | a new commercial kit for diagnosis of bovine paratuberculosis (johne's disease), called the johne's absorbed eia (enzyme immunoassay; commonwealth serum laboratories, parkville, victoria, australia), was evaluated by using serum specimens from the national repository for paratuberculosis specimens. the evaluation was specifically designed to measure test sensitivity and specificity for detection of dairy cattle with subclinical paratuberculosis. the case definition of subclinical bovine paratube ... | 1991 | 2007634 |
| serodiagnosis of bovine paratuberculosis by use of a dot enzyme-linked immunosorbent assay. | a dot elisa was developed for detection of antibodies to mycobacterium paratuberculosis. the assay was evaluated by testing sera from cattle that were determined, by bacteriologic culturing of feces, to be infected with m paratuberculosis and were suspected of having clinical disease. further evaluation involved testing sera from cattle in which m paratuberculosis had not been isolated from feces on several attempts. results of the dot elisa were positive for sera from 86 of 101 infected cattle, ... | 1991 | 2012331 |
| a dna probe for the detection of mycobacterium paratuberculosis. | a genomic library of dna extracted from mycobacterium paratuberculosis was constructed in the expression vector lambda gt 11. the library was screened by plaque hybridization with labelled m. paratuberculosis genomic dna as probe. strongly hybridizing plaques were isolated and their dna extracted and characterised for m. paratuberculosis specificity by hybridization to dna from other mycobacteriaceae. a clone was obtained which was specific for m. paratuberculosis. dna from this clone could dete ... | 1991 | 2024441 |
| gen-probe rapid diagnostic system for the mycobacterium avium complex does not distinguish between mycobacterium avium and mycobacterium paratuberculosis. | three reference and 16 field strains of mycobacterium paratuberculosis were tested with the gen-probe mycobacterium avium complex dna probe (gen-probe inc., san diego, calif.). all reference strains and 12 of 16 field strains gave positive hybridization results with the probe. this study shows that the m. avium complex probe does not distinguish between m. avium and m. paratuberculosis and indicates heterogeneity in the 16s rrna gene of m. paratuberculosis. | 1991 | 2037681 |
| antibody reactivities of mycobacterium paratuberculosis infected sheep as analyzed by enzyme-linked immunosorbent assay and western blotting. | antibody reactivities in sera from mycobacterium paratuberculosis (m. ptb) infected and vaccinated sheep were analyzed by enzyme-linked immunosorbent assay (elisa) and western (immuno)blotting using a sonicate antigen from m. ptb. both methods allowed good differentiation between infected/vaccinated animals and noninfected controls. removal of nonspecific crossreactive antibodies by absorption with a m. phlei sonicate antigen coupled to sepharose reduced elisa reactivities of positive sera by 50 ... | 1991 | 2040422 |
| mycobacterium paratuberculosis monoassociated nude mice as a paratuberculosis model. | in this study, a paratuberculosis (johne's disease) model was developed by intragastrically dosing gnotobiotic athymic nude mice with mycobacterium paratuberculosis. the mice infrequently shed bacilli from their intestinal tracts during the first 4 months after inoculation. following this time, increasing numbers of m. paratuberculosis (greater than 4.0 log10 bacilli per fecal pellet by 40 weeks) were recovered from the feces of the 12 mice that remained in the isolator. a similar pattern of rec ... | 1991 | 2063515 |
| effects of colonial morphology and tween 80 on antimicrobial susceptibility of mycobacterium paratuberculosis. | smooth (sm) and rough (rg) colonial types of mycobacterium paratuberculosis atcc 19698 and two clinical isolates were tested to examine their growth responses in medium containing antimicrobial agents. susceptibility tests were done in middlebrook 7h12b medium with and without tween 80 and one of the following antimicrobial agents: streptomycin, isoniazid, rifampin, ethambutol, ciprofloxacin, and penicillin g. growth responses in the presence of antimicrobial agents led to the following observat ... | 1990 | 2088185 |
| immunohistochemical study of bovine lymph nodes with antibodies against s100 protein subunits: comparison between lymph nodes of healthy and mycobacterium paratuberculosis-infected cattle. | using immunohistochemistry, the differential distribution of the alpha subunit (s100 alpha) and beta subunit (s100 beta) of s100 protein was studied in mesenteric lymph nodes from normal or mycobacterium paratuberculosis-infected cattle. in epithelioid cell granulomas, s100 alpha-positive epithelioid cells and some giant cells were scattered among s100 alpha-negative cells, which were predominant. the s100 beta-positive and -negative cells contained acid-fast bacilli. the presence of s100 beta-p ... | 1990 | 2089532 |
| cyclophosphamide and prednisolone exacerbate the severity of intestinal paratuberculosis in mycobacterium paratuberculosis monoassociated mice. | in this study we examined the effects of continuous oral administration of the immunosuppressive agents cyclophosphamide and prednisolone on the susceptibility of gnotobiotic nu/+ balb/c mice to intestinal paratuberculosis. treatment with either cyclophosphamide or prednisolone led to fecal shedding of mycobacterium paratuberculosis, and increased the numbers of m. paratuberculosis recovered from the intestinal tract and liver, to levels similar to those recovered from untreated nu/nu mice. nume ... | 1990 | 2097495 |
| gas chromatographic characterization of the relationship between some mycobacterium avium and mycobacterium paratuberculosis strains. | mycobacterium avium strain p-55 and m. avium strain dent differ from m. avium strain 16909-338 on the basis of their fatty acid spectra (c14:0, c18:0 and tuberculostearic [tbs] acids) studied by multivariate statistical analyses. strains p-55 and dent are closer to m. paratuberculosis strain 5889 than to m. avium strain 16909-338, a finding which is in harmony with earlier immunological observations. the recently isolated m. paratuberculosis strain 385 has proved different from m. paratuberculos ... | 1990 | 2099604 |
| diagnostic value of intravenously administered johnin purified protein derivative (ppd) in bovine paratuberculosis. | the diagnostic value of intravenously administered johnin purified protein derivative (ppd) was studied in 45 cattle of different age, coming from herds infected by, or free from, mycobacterium paratuberculosis. in addition to observing the clinical symptoms, the animals' sera were assayed for specific antibodies by the complement fixation (cft) and immunodiffusion (agid) tests. the blastogenic transformation of peripheral lymphocytes was determined on the basis of 3htdr incorporation. changes i ... | 1990 | 2100937 |
| comparison of the complement-fixation and agar gel immunodiffusion tests for diagnosis of subclinical bovine paratuberculosis. | the performance of the serum complement fixation (cf) test was compared with that of a serum agar gel immunodiffusion (agid) test on 74 subclinically infected and 154 uninfected cattle in 6 commercial midwestern dairy herds with mycobacterium paratuberculosis infection and on 30 cattle in a herd that was free of infection. infection status of cattle within herds was established by performance of a series of 3 or more fecal cultures and of ileocecal lymph node cultures of culled cattle. in cattle ... | 1990 | 2107777 |
| experimental infection of calves with an apparently specific goat-pathogenic strain of mycobacterium paratuberculosis. | four calves and four goat kids were inoculated perorally with a norwegian goat-pathogenic strain of mycobacterium paratuberculosis. none of the calves developed clinical disease, pathological lesions or humoral antibody response, but the organism was reisolated from the small intestine and mesenteric lymph nodes when the calves were slaughtered after 7, 12, 14 and 18 months, respectively. as one of the goats died of non-specific causes and one did not become infected, two remained as positive co ... | 1990 | 2108999 |
| t-cellular immune reactions (in macrophage inhibition factor assay) against mycobacterium paratuberculosis, mycobacterium kansasii, mycobacterium tuberculosis, mycobacterium avium in patients with chronic inflammatory bowel disease. | a mycobacterial aetiology has been suggested for crohn's disease. a slow growing mycobacterium, biochemically and genetically identical to m paratuberculosis, the causative agent of enteritis in ruminants (johne's disease), has been isolated from gut specimens of patients affected by crohn's disease. if m paratuberculosis or other mycobacteria play a role in the pathogenesis of crohn's disease, then patients may have been sensitised to these mycobacteria or show an anergy immune reaction. we the ... | 1990 | 2112502 |
| paratuberculosis in a cattle herd: comparison of allergic, serologic and faecal microscopic tests. | in a beef herd of 230 cows kept on pasture and infected with mycobacterium paratuberculosis, faecal samples were tested microscopically and the blood serum by the complement fixation and the immunodiffusion test. intradermal tests were carried out simultaneously with bovine and avian tuberculin as well as with two dilutions (0.86 mg/ml and 0.50 mg/ml tuberculoprotein) of paratuberculin. in the tested herd 35.2% of the cows gave positive faecal tests while 10 and 9.1% were positive in the agar ge ... | 1990 | 2125397 |
| isolation of mycobacterium paratuberculosis from the uterine flush fluids of cows with clinical paratuberculosis. | mycobacterium paratuberculosis was isolated from the uterine flush fluids obtained from 3 to 4 cows with clinical paratuberculosis. four cows with clinical paratuberculosis were subjected to uterine lavage, using an established embryo recovery technique, and the recovered fluids were cultured for m paratuberculosis. mycobacterium paratuberculosis has previously been demonstrated to adhere to bovine ova. embryos within the uterus of a superovulated cow infected with m paratuberculosis could be ex ... | 1990 | 2125588 |
| identification of two groups of mycobacterium paratuberculosis strains by restriction endonuclease analysis and dna hybridization. | genomic dna was prepared from four reference strains of mycobacterium paratuberculosis and 46 isolates of this organism from new zealand, australia, canada, and norway and also from two mycobactin-dependent "wood pigeon" strains. the dna was characterized by restriction endonuclease analysis, both with and without dna hybridization, with a probe specific to a repetitive dna sequence in m. paratuberculosis. both techniques differentiated m. paratuberculosis strains into two groups, but dna hybrid ... | 1990 | 2166089 |
| molecular biology of crohn's disease mycobacteria. | a glasgow surgeon, t.k. dalziel, published a detailed description of chronic enteritis in humans in 1913. he proposed that the disease was caused by the same organisms as those responsible for chronic enteritis, johne's disease, in animals described a few years earlier (1895). dalziel's dilemma was that he could see acid-fast bacilli in the diseased animal tissues but not in the diseased human tissues. little real progress in the medical understanding of the causes of chronic enteritis in humans ... | 1990 | 2169929 |
| isolation of mycobacterium paratuberculosis from washed bovine ova after in vitro exposure. | to establish whether mycobacterium paratuberculosis could be cultured from dulbecco phosphate-buffered saline solution (dpbss) and to test 3 sampling methods, dpbss supplemented with 2% fetal bovine serum was inoculated with m paratuberculosis at concentrations of 10(4), 10(3), 10(2), 10(1), and 10(0) colony-forming units/ml. the inoculated media was sampled after mixing, after centrifugation, and after centrifugation and decontamination with 0.75% hexadecylpyridinium chloride. the samples were ... | 1990 | 2186676 |
| protein g-based enzyme-linked immunosorbent assay for anti-mpb70 antibodies in bovine tuberculosis. | mpb70 is a highly species specific protein which is secreted from mycobacterium bovis during culture. to investigate whether antibodies against mpb70 can be used as an indicator of infection with m. bovis, an enzyme-linked immunosorbent assay was developed, based on the use of biotinylated protein g, to provide a common indicator for antibody formation in different species. during experimental infection with m. bovis in cattle, a characteristic pattern of anti-mpb70 antibody production was obser ... | 1990 | 2191012 |
| preliminary observations on ovine paratuberculosis (johne's disease) in zambia. | in an imported flock of sheep from south africa, an ewe became partially anorexic and gradually losing weight and conditions. the paratuberculosis (johne's disease) was confirmed on clinical, faecal and histopathological examination. cultural examination remained doubtful. serological investigation of other sheep in the flock and at other farm reacted to antibodies of mycobacterium paratuberculosis on complement fixation test. the study suggests that the disease is actively spreading. this is th ... | 1990 | 2218037 |
| characterization of mycobacterium paratuberculosis and "wood-pigeon" mycobacteria by isoenzyme profile and selective staining of immunoprecipitates. | cell-free extracts of various strains belonging to mycobacterium paratuberculosis (ptb) and "wood-pigeon" (wp) mycobacteria were compared by polyacrylamide gel electrophoresis and the various protein bands obtained were tested for peroxidase enzyme activity. one strain of mycobacterium avium served as a control. bacterial extracts were also analysed by crossed immunoelectrophoresis (criep) and fused rocket immunoelectrophoresis (friep) using antisera raised in rabbit against m. paratuberculosis ... | 1990 | 2218060 |
| genetic regulation of mycobacterium paratuberculosis infection in recombinant inbred mice. | 1990 | 2238389 | |
| effect of polyoxyethylene sorbate compounds (tweens) on colonial morphology, growth, and ultrastructure of mycobacterium paratuberculosis. | polyoxyethylene sorbate (tween) compounds were tested to compare their growth stimulation effects on mycobacterium paratuberculosis. three low passage and three high passage clinical isolates and atcc strain 19698 were used. tween 20, 40, 60, and 80 were tested at concentrations of 0, 0.001, 0.01, 1.0, and 3.0% (w/v) in radiometric broth culture media and in middlebrook 7h9 agar plates. scanning and transmission electron microscopy were used to examine cell wall appearance and ultrastructure, re ... | 1990 | 2245009 |
| enhanced radiometric detection of mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens. | a commercial radiometric medium, bactec 12b, was modified by addition of mycobactin, egg yolk suspension, and antibiotics (vancomycin, amphotericin b, and nalidixic acid). decontaminated bovine fecal specimens were filter concentrated by using 3-microns-pore-size, 13-mm-diameter polycarbonate filters, and the entire filter was placed into the radiometric broth. comparison of the radiometric technique with conventional methods on 603 cattle from 9 mycobacterium paratuberculosis-infected herds fou ... | 1990 | 2254428 |
| the sensitivity and specificity of a modified elisa for the diagnosis of johne's disease from a field trial in cattle. | an enzyme-linked immunosorbent assay (elisa) for the detection of antibodies against mycobacterium paratuberculosis in cattle was evaluated in three herds known to have johne's disease. prior to testing, the plasma was absorbed with dried mycobacterium phlei in order to remove cross-reacting antibody specificities. the sensitivity and specificity of the elisa were calculated after repeatedly testing 327 cattle in the infected herds. of these, 53 animals had one or more positive faecal cultures o ... | 1990 | 2281604 |
| probable paratuberculosis in a sicilian ass. | a presumptive diagnosis of paratuberculosis was made in a sicilian ass on the basis of a history of chronic diarrhea and weight loss, pasture exposure to a heifer with paratuberculosis confirmed by bacterial culture of feces, postmortem identification of granulomatous inflammation of the intestine containing acid-fast organisms, the absence of acid-fast organisms in extraenteric tissues, and the absence of exposure to tuberculosis. the literature on paratuberculosis in equids is reviewed. the po ... | 1990 | 2298679 |
| bactericidal activities of various antimicrobial agents against human and animal isolates of mycobacterium paratuberculosis. | the mics and mbcs of various antimicrobial agents for strains of mycobacterium paratuberculosis isolated from animal and human sources were evaluated. the mics and mbcs of rifabutin, ciprofloxacin, ethambutol, clofazimine, streptomycin, cefazolin, and amikacin were found to be well below therapeutic levels in serum and tissue. | 1990 | 2327783 |
| use of an enzyme-linked immunosorbent assay to estimate prevalence of paratuberculosis in cattle of florida. | a survey was conducted from 1986 through 1987, for which an elisa was used to obtain information on the prevalence of mycobacterium paratuberculosis infection in cattle of florida. results revealed prevalence of 8.6% in beef cattle and 17.1% in dairy cattle. in beef and dairy cattle, prevalence increased with increasing herd size. it was concluded that elisa-detectable circulating antibodies to m paratuberculosis are widespread in cattle of florida. | 1990 | 2332370 |
| growth of mycobacterium paratuberculosis in radiometric, middlebrook and egg-based media. | the ability of bactec radiometric 7h12 broth, middlebrook 7h10 tween broth, middlebrook 7h10 agar, and herrold's egg-yolk medium to provide early detection of mycobacterium paratuberculosis was evaluated. the minimum detection times in days for the various media were: 7h12, 9; 7h10 agar, 23 (plate), 28 (slant); 7h10 tween broth; 27; and herrold's egg-yolk medium, 43 (plate), 49 (slant). the radiometric broths provided the earliest detection of m. paratuberculosis, and 3625 organisms ml-1 were re ... | 1990 | 2336785 |
| the pathology of johne's disease in sheep. | the clinical, gross and histopathological findings in 50 sheep affected with johne's disease are described. clinically 90% were emaciated and 20% showed severe diarrhoea. on necropsy there was thickening of the walls of the intestines, particularly of the ileum, caecum and less frequently the jejunum, but in 36% of sheep the changes were only mild. histologically there was a granulomatous enteritis, typhlitis and colitis, with the most severe changes in the terminal ileum. high numbers of acid-f ... | 1990 | 2344336 |
| use of highly specific dna probes and the polymerase chain reaction to detect mycobacterium paratuberculosis in johne's disease. | dna probes that hybridize to a mycobacterial insertion sequence, is900, present in multiple copies in the genome of mycobacterium paratuberculosis were found to be highly specific for m. paratuberculosis. dna sequences derived from is900 were used to prepare dna primers for detection and identification of m. paratuberculosis by the polymerase chain reaction. highly specific direct detection of m. paratuberculosis dna in feces from cattle with johne's disease was obtained. the polymerase chain re ... | 1990 | 2351737 |
| impaired humoral immune responses to mycobacterial antigen in aged murine gut-associated lymphoid tissues. | senescence-related alterations of local gut mucosal immune responses to enteric mycobacterial antigen (ag) were examined. both aged (greater than 24 months old) and young adult (4-5 months old) balb/c mice were enterically immunized with crude mycobacterium paratuberculosis (m. paratbc) protoplasmic ag, and in vitro ag- and class-specific immunoglobulin (g) production by lymphocytes from gut-associated lymphoid tissues (galt) (peyer's patches, pp; mesenteric lymph nodes, mln) and non-galt (splee ... | 1990 | 2362471 |
| in vivo immunologic intervention in age-related t cell defects in murine gut-associated lymphoid tissues by il2. | our recent studies indicate that the aging process impairs gut mucosal humoral immune responses to mycobacterial antigen (ag), largely owing to defects in t cell function--in particular, that of suppressor t cells. to correct the age-associated ag-specific t cell-mediated immune alteration recombinant il2 (50,000 units/s.c./mouse/day) was administered for 3 weeks to the aged (greater than 24 months) mice (balb/c), which were divided into 4 groups (gr) [gr. 1, fed intragastrically (i.g.) with sal ... | 1990 | 2362472 |
| granulomatous enteritis following oral inoculation of newborn rabbits with mycobacterium paratuberculosis of bovine origin. | to assess the rabbit as a model for the study of paratuberculosis infection, two groups of newborn rabbits were orally inoculated at one to two days of age with mycobacterium paratuberculosis (atcc 19698 or field strain 22206) and compared to uninoculated controls. nine of thirteen rabbits (69%) inoculated with atcc 19698, and all three rabbits inoculated with 22206, experienced episodes of intermittent diarrhea starting four months after inoculation. multifocal granulomas containing acid-fast o ... | 1990 | 2379110 |
| immunohistochemical methods for the visualization of mycobacterium paratuberculosis in bovine tissues. | the peroxidase-antiperoxidase (pap), streptavidin-biotin (sb), and avidin-biotin-complex (abc) techniques have been evaluated for the visualization of mycobacterium paratuberculosis (mp) in formalin-fixed, paraffin-embedded bovine tissues. the used immunoperoxidase techniques were comparatively better than the ziehl-neelsen stain, specially for the demonstration of small number of mycobacteria in tissue sections. | 1990 | 2382517 |
| some diagnostic features of the pathogenesis of bovine paratuberculosis (johne's disease) and serum biochemical changes after oral reinfection. | ten bull-calves were infected with 10(8) viable cells of mycobacterium paratuberculosis per os. during the 400-day period of observation faecal and blood samples were taken from animals at 30-day intervals. faecal samples were examined microscopically, blood samples by the cft, agid and lst tests. intradermal allergic tests were carried out at pi (post infection) days 92, 217, 336, using mammalian, avian and johnin ppd. in the period of study, these efficiency indices showed fluctuations charact ... | 1990 | 2385984 |
| numerical taxonomy of mycobactin-dependent mycobacteria, emended description of mycobacterium avium, and description of mycobacterium avium subsp. avium subsp. nov., mycobacterium avium subsp. paratuberculosis subsp. nov., and mycobacterium avium subsp. silvaticum subsp. nov. | we performed a numerical taxonomy analysis of 38 mycobacterium paratuberculosis and related mycobacterial strains, including wood pigeon mycobacteria; this analysis was based on 22 tests, which were selected for their potential discriminative value from a total of 51 tests studied and produced four well-defined clusters. cluster 1 contained the m. paratuberculosis strains, including two strains isolated from crohn's disease patients; cluster 2 contained mycobacterium avium and mycobacterium intr ... | 1990 | 2397193 |
| rapid lysis technique for mycobacterial species. | the lysis of mycobacterial cells typically has been difficult and time-consuming. we report a method for the physical rupture of mycobacterium paratuberculosis and several other members of the family mycobacteriaceae using a mini-beadbeater cell disrupter (biospec products; bartlesville, okla.) and zirconium beads, a process which yields dna and rna of high molecular weight and in greater quantity than that obtained by rupture in a ribi pressure cell. | 1987 | 2447121 |
| antigenic relationship between mycobacterium paratuberculosis and mycobacterium avium. | four prototype strains of mycobacterium paratuberculosis contained the type-specific glycopeptidolipid antigen of serovar 8 of the m avium complex. this glycolipid was distinguished by a 4,6-(1'-carboxyethylidene)-3-o-methyl-beta-d-glucopyranosyl terminal unit. of 59 low-passage, field isolates of m paratuberculosis, 2 contained this antigen, and these 2 isolates were indistinguishable from m avium serovar 8. however, most m paratuberculosis isolates had no characteristic surface glycopeptidolip ... | 1988 | 2459998 |
| control of johne's disease in four commercial dairy herds in iowa. | a 6-year study was conducted in 4 dairy herds in iowa in which johne's disease was diagnosed previously. fecal specimens were collected at 6-month intervals from animals 2 years of age and over for mycobacteriologic examination. serum samples were obtained at 3-month intervals and tested by enzyme-linked immunosorbent assay (elisa). the antigen used in the elisa was a potassium chloride extract of a field strain of mycobacterium paratuberculosis. the elisa reactions were observed in 87% of the c ... | 1989 | 2488348 |
| paratuberculosis in cattle: a comparison of three serologic tests with results of fecal culture. | feces and blood were collected from cattle in 13 herds known to be infected with mycobacterium paratuberculosis to evaluate a complement-fixation (cf) test, an agar gel immunodiffusion (agid) test and an enzyme-linked immunosorbent assay (elisa) for the serologic diagnosis of paratuberculosis. m. paratuberculosis was isolated from the feces of 36 of 192 cattle examined. twenty-three culture-positive animals had cf test titers regarded as suspect or positive, 10 were positive by the agid test and ... | 1989 | 2495699 |
| evaluation of the agar gel immunodiffusion test for diagnosis of subclinical paratuberculosis in cattle. | concurrent bacteriologic culture of feces and agar gel immunodiffusion (agid) testing was performed on all cows and bred heifers over 14 months old in 10 dairy herds during a 32-month period to determine the effectiveness of the agid test for the detection of subclinical paratuberculosis. herds were sampled 5 times and, when possible, culled animals were tested again at slaughter. during 5 herd-wide samplings, mycobacterium paratuberculosis was isolated from 139 fecal specimens obtained from 109 ... | 1989 | 2496629 |
| comparative intracellular growth of difficult-to-grow and other mycobacteria in a macrophage cell line. | we have recently developed a murine macrophage cell line (j-774) model which permits the growth of various mycobacteria (8). the purpose of the present investigation was to compare the intracellular growth of various difficult-to-grow mycobacteria (mycobacterium paratuberculosis, m. ulcerans), and other pathogenic (m. tuberculosis h37rv, m. kansasii, m. bovis) and nonpathogenic or avirulent (m. tuberculosis h37ra, m. bovis bcg, m. gastri) mycobacteria. electron microscopic studies were also perf ... | 1989 | 2503976 |
| genetic relatedness among mycobacterium paratuberculosis and m. avium complex. | total dna was extracted from m. paratuberculosis (atcc 19698) and from m. avium complex (atcc 25291) cultivated on rvb-10 enriched liquid media. restriction endonuclease analysis of total dna was performed with 34 enzymes and dna digestion profiles were compared. fifteen enzymes revealed important differences between the two species. two pairs of enzymes (ecorii, bstni) and (mboi, sau3ai) provide evidence for the presence of dcmi and dam methylation in dna of m. avium complex and m. paratubercul ... | 1989 | 2503996 |
| serodiagnosis of ovine paratuberculosis, using lipoarabinomannan in an enzyme-linked immunosorbent assay. | the use of lipoarabinomannan (lam; obtained from mycobacterium paratuberculosis) in and elisa (lam-elisa) to test 75 sheep sera from a paratuberculosis-infected flock resulted in an approximate threefold increase in sensitivity (from 23.5% to 70.6%), compared with the use of annau's polysaccharide in a complement fixation test (p-cft). even after manipulation of the lam-elisa cut-off value to produce a specificity of 100% to match that of the p-cft, the sensitivity still was approximately twofol ... | 1989 | 2504085 |
| detection and identification of mycobacteria by amplification of mycobacterial dna. | a 383bp segment of the gene coding for the 65kd mycobacterial antigens from mycobacterium tuberculosis, mycobacterium bovis, mycobacterium avium, mycobacterium paratuberculosis, and mycobacterium fortuitum was amplified using taq polymerase and synthetic oligonucleotide primers and the amplified dnas from four of these species were compared by nucleotide sequencing. although the gene segments from these species showed considerable similarity, oligonucleotide probes which could distinguish m. tub ... | 1989 | 2507865 |
| comparison of dot immunobinding assay, enzyme-linked immunosorbent assay and immunodiffusion for serodiagnosis of paratuberculosis. | a rapid, simple and inexpensive dot immunobinding assay (dia) was evaluated for the serodiagnosis of paratuberculosis in cattle. the assay was performed on nitrocellulose strips which were dotted with purified protoplasmic antigen of mycobacterium paratuberculosis. after incubation with test serum samples, the bound antibodies were detected using an enzyme-amplified immunostaining procedure. the efficacy of dia as a screening test for paratuberculosis was compared to that of an enzyme-linked imm ... | 1989 | 2512004 |
| insertion elements in mycobacteria. | we have isolated and characterised a repetitive element from the genome of mycobacterium paratuberculosis. this repetitive element shows many features characteristic of a bacterial insertion element. | 1989 | 2548361 |
| sequence and characteristics of is900, an insertion element identified in a human crohn's disease isolate of mycobacterium paratuberculosis. | the complete sequence of an insertion element is900 in mycobacterium paratuberculosis is reported. this is the first characterised example of a mycobacterial insertion element. is900 consists of 1451bp of which 66% is g + c. it lacks terminal inverted and direct repeats, characteristic of escherichia coli insertion elements but shows a degree of target sequence specificity. a single open reading frame (orf 1197) coding for 399 amino acids is predicted. this amino acid sequence, and to a lesser e ... | 1989 | 2555783 |
| dna probes demonstrate a single highly conserved strain of mycobacterium avium infecting aids patients. | strains of the mycobacterium avium intracellulare complex (maic) have become important colonisers of patients with acquired immunodeficiency syndrome (aids). restriction fragment length polymorphisms were used to study the dna from 88 maic isolates, including 51 derived from 47 aids patients. maic isolates from 33 of 45 aids patients were identical at the molecular level and distinct from the mycobacteria isolated from the stools of healthy subjects. the study also showed that serotyping correla ... | 1989 | 2562881 |
| the genetic relationship between mycobacterium paratuberculosis and the m. avium complex. | 1989 | 2569802 | |
| dna polymorphism in mycobacterium paratuberculosis, "wood pigeon mycobacteria," and related mycobacteria analyzed by field inversion gel electrophoresis. | mycobacterium paratuberculosis strains, mycobacteria from patients suffering from crohn's disease, "wood pigeon mycobacteria," and representatives of mycobacterium avium-mycobacterium intracellulare were compared by restriction endonuclease drai digestion and field inversion gel electrophoresis. characteristic profiles were seen for m. paratuberculosis, including isolates from patients suffering from crohn's disease, for wood pigeon mycobacteria, and for m. avium-m. intracellulare serotypes 2, 1 ... | 1989 | 2574186 |
| crohn's disease and the mycobacterioses: a review and comparison of two disease entities. | crohn's disease is a chronic granulomatous ileocolitis, of unknown etiology, which generally affects the patient during the prime of life. medical treatment is supportive at best, and patients afflicted with this disorder generally live with chronic pain, in and out of hospitals, throughout their lives. the disease bears the name of the investigator who convincingly distinguished this disease from intestinal tuberculosis in 1932. this distinction was not universally accepted, and the notion of a ... | 1989 | 2644025 |
| immunohistochemical examination for mycobacteria in intestinal tissues from patients with crohn's disease. | we conducted an immunohistochemical search for mycobacteria in the intestinal tissues of patients with crohn's disease. tissues obtained by biopsy or surgical resection and fixed by a variety of methods (formalin, periodate-lysine-paraformaldehyde, fresh-frozen) were reacted by an immunoperoxidase method with antibodies to (a) mycobacterium paratuberculosis strain linda, (b) m. tuberculosis, and (c) the common mycobacterial antigen, lipoarabinomannan. each of the antibody preparations was shown ... | 1989 | 2647572 |
| comparison of two methods for isolation of mycobacterium paratuberculosis from bovine fecal samples. | fecal samples from 131 cattle clinically suspect for paratuberculosis were cultured bacteriologically, using the traditional sedimentation processing method and a processing method that included a centrifugation step. of 16 samples that were contaminated, 6 were culture-positive on at least 1 medium and by 1 processing method. ten of 131 (7.6%) fecal samples processed by both methods were lost because of contamination. the number of culture-positive samples (using both processing methods) were 6 ... | 1989 | 2672913 |
| the specificity of antibody response in experimental and natural bovine paratuberculosis studied by crossed immunoelectrophoresis with intermediate gel. | the sonicate antigen (mps) of a local strain (ivri) of mycobacterium paratuberculosis and a commercial lysate of strain 18 were analysed using hyperimmune rabbit and calf antisera to mps in crossed immunoelectrophoresis with intermediate gel (cie-ig) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). the rabbit antiserum was more potent than the calf antiserum and it precipitated 35 and 15 antigens, respectively, among mps and lysate antigens. sds-page resolved 50 and 32 ... | 1989 | 2696199 |
| sequential bacteriological observations in relation to cell-mediated and humoral antibody responses of cattle infected with mycobacterium paratuberculosis and maintained on normal or high iron intake. | twenty calves were orally infected with mycobacterium paratuberculosis before weaning. ten of these plus 4 non-infected controls were maintained on elevated dietary iron intake from 6 to 33 months of age. during this time, in which the majority of animals were bred, the influence of increased dietary iron upon tests of cellular and humoral immune responsiveness to antigens of the organism were monitored. results were examined in relation to the organism's capacity to multiply and infect up to 7 ... | 1989 | 2712768 |
| bovine fetal infection with mycobacterium paratuberculosis. | in utero transmission of mycobacterium paratuberculosis, the causative agent of paratuberculosis in cattle, has been suggested. tissue specimens were obtained at a packing plant from pregnant dairy cows and their fetuses and from cows with clinical signs of paratuberculosis and from their fetuses. specimens were processed according to methods described for isolating m paratuberculosis from bovine tissues and were incubated on herrold egg yolk medium for 16 weeks. presumed positive specimens were ... | 1989 | 2722634 |
| effects of altered dietary iron intake in mycobacterium paratuberculosis-infected dairy cattle: sequential observations on growth, iron and copper metabolism and development of paratuberculosis. | twenty calves were orally inoculated with mycobacterium paratuberculosis at six weeks old. at six months old, 10 of these, plus four uninfected controls were maintained on limited dietary copper and supplemented iron intake for a further 27 months. during this time all these animals, together with a further four untreated controls, were bred before being killed and examined for evidence of paratuberculosis. despite significant reduction in weight gain, attributable to both iron supplementation a ... | 1989 | 2740623 |
| development of a diagnostic test for johne's disease using a dna hybridization probe. | a dna probe, m13 mphaw71, that detects mycobacterium paratuberculosis in the fecal material of infected animals was developed for use in the diagnosis of johne's disease. the probe detected as few as 10(5) m. paratuberculosis when hybridized under stringent conditions to total genomic dna purified from bovine fecal material. when the probe was used diagnostically, it did not differentiate members of the mycobacterium avium-m. intracellulare-m. paratuberculosis complex. compared with culturing, t ... | 1989 | 2768445 |
| experimental paratuberculosis (johne's disease)--studies on biochemical parameters in cattle. | ten male holstein-friesian calves naturally infected by mycobacterium paratuberculosis were experimentally re-infected orally at an average of 17 days. monthly measurements were conduced of the following activities, in the period between post infection days 160 and 400: total protein (tpr), albumin (alb), cholesterol (chol), triglycerides (trig), zn and cu concentrations as well as sorbitol dehydrogenase, lactate dehydrogenase (ldh), alpha-hydroxybutyrate dehydrogenase (alpha-hbdh), gamma-glutam ... | 1989 | 2774844 |
| identification of a repetitive dna sequence specific to mycobacterium paratuberculosis. | a 0.2-kb dna sequence specific to mycobacterium paratuberculosis, the causative organism of johne's disease, was isolated from a partial genomic library. the sequence was part of a larger repetitive dna element and was present in strains of m. paratuberculosis from cattle, sheep, goat, deer and also a woman with crohn's disease but not in m. paratuberculosis strain 18. the sequence was not present in strains of 19 other mycobacterial species including 31 reference serotype strains of the m. aviu ... | 1989 | 2777063 |
| analysis of restriction endonuclease fragment patterns of dna from mycobacterium paratuberculosis. | the dna from 31 isolates and a reference strain of mycobacterium paratuberculosis was digested individually with restriction endonucleases bste ii and pst i. dna fragments were separated by gel electrophoresis and analyzed. the isolates were from 23 american states, argentina and nova scotia. twenty-seven were isolated from cattle, two from goats and two from sheep. with the exception of one isolate from cattle, all had restriction endonuclease fragment patterns identical to the fragment pattern ... | 1989 | 2784928 |
| a rabbit model for study of mycobacterium paratuberculosis infection. | of 21 newborn rabbits inoculated orally with mycobacterium paratuberculosis atcc 19698, 13 (62%) became infected, as determined by histopathology and culture. of the 21 inoculated rabbits, 14 (67%) experienced episodes of intermittent diarrhea, sometimes as early as 5 months after inoculation. feces varied in consistency from soft-semisolid to watery. the organism was isolated from the sacculus rotundus, vermiform appendix of the cecum, ileum, mesenteric lymph node, and feces of 9 of 21 (43%) m. ... | 1989 | 2807547 |
| a modified elisa for the detection of goats infected with mycobacterium paratuberculosis. | 1989 | 2818377 | |
| cytokine regulation of the intracellular growth of mycobacterium paratuberculosis in bovine monocytes. | in this study we examined the influence of various crude and recombinant cytokines on the ingestion and intracellular survival of mycobacterium paratuberculosis within bovine monocytes and monocyte-derived macrophages. cytokine pretreatment had little effect on the ingestion of m. paratuberculosis by bovine monocytes and macrophages. monocytes that were continuously incubated with virus-induced crude bovine interferon (100 u) or recombinant bovine alpha interferon (100 u) significantly restricte ... | 1988 | 2838423 |
| characterization of total deoxyribonucleic acid of mycobacterium paratuberculosis (atcc 19698) and of m. avium complex (atcc 25291) using restriction enzymes. | total dna was extracted from m. paratuberculosis (atcc 19698) and from m. avium complex (atcc 25291) cultivated on rvb-10 enriched liquid media. restriction endonuclease analysis was conducted of total dna using 34 enzymes and dna digestion profiles were compared. fifteen enzymes revealed important differences between the two species. two pairs of enzymes (ecorii, bstni) and (mboi, sau3ai) provide evidence for the presence of dcmi and dam methylation in dna of m. avium complex and m. paratubercu ... | 1988 | 2854962 |
| crohn's disease-isolated mycobacteria are identical to mycobacterium paratuberculosis, as determined by dna probes that distinguish between mycobacterial species. | dna extracted from an unclassified crohn's disease-isolated mycobacterium strain was cloned. the recombinant clones were radiolabeled and hybridized to restriction digests of mycobacterial dna transferred to nylon membranes. restriction fragment length polymorphisms (rflps) were identified that distinguished between mycobacterial dna samples. quantitative estimates of frequencies of dna base substitution were also obtained. no rflps were detected between the dna of three unclassified crohn's dis ... | 1987 | 2884232 |
| the use of dna probes identifying restriction-fragment-length polymorphisms to examine the mycobacterium avium complex. | dna probes were used to identify restriction-fragment-length polymorphisms (rflps) in dna samples, demonstrating that the mycobacterium avium complex could be clearly divided into m. avium and mycobacterium intracellulare strains. less than 2% dna base substitution was found between m. avium strains, whereas the m. intracellulare strains had greater than 15% base substitution. the johne's disease bacillus, mycobacterium paratuberculosis (american type strain), was found to be distinguishable fro ... | 1987 | 2896288 |
| intestinal multiplication of mycobacterium paratuberculosis in athymic nude gnotobiotic mice. | in this study gnotobiotic mice were inoculated with a human isolate of mycobacterium paratuberculosis (strain linda; atcc 43015) in an attempt to investigate the pathogenesis of intestinal paratuberculosis. mycobacterium paratuberculosis-monoassociated nu/+ mice developed a persistent low-level intestinal infection but did not support progressive bacillary multiplication. in contrast, monoassociated nu/nu mice eventually harbored approximately 10(7) m. paratuberculosis per g of intestinal tissue ... | 1989 | 2909488 |
| infection experiments with mycobacterium paratuberculosis strains. | 1986 | 2953190 | |
| restriction endonuclease analysis of various strains of mycobacterium paratuberculosis isolated from cattle. | deoxyribonucleic acid (dna) preparations from 3 reference strains of mycobacterium paratuberculosis and from 23 isolates of m paratuberculosis obtained from cattle in new zealand were characterized by restriction endonuclease analysis, using the enzymes bste ii, pvu ii, and bcl i. patterns of dna fragments for strain 18 (one of the reference strains) differed markedly from patterns of other strains, indicating genetic differences between strain 18 and the other strains of m paratuberculosis eval ... | 1986 | 3022622 |
| isolation and analysis of restriction endonuclease digestive patterns of chromosomal dna from mycobacterium paratuberculosis and other mycobacterium species. | a relatively rapid and efficient method for the extraction of chromosomal dna from mycobacterium paratuberculosis and other mycobacteria was developed. approximately 25 to 50 micrograms of dna could be extracted from 100 mg (wet weight) of cells, which was sufficient to perform several restriction endonuclease analyses from a single preparation. the dna from five mycobacterium species, including four strains of m. paratuberculosis and four strains of m. avium, was analyzed by this method. digest ... | 1987 | 3040802 |
| immunodiffusion analysis shows that mycobacterium paratuberculosis and other mycobactin-dependent mycobacteria are variants of mycobacterium avium. | antigenic analysis by immunodiffusion has been applied to 74 strains of mycobactin-dependent mycobacteria. thirty-eight strains were of mycobacterium paratuberculosis from cases of johne's disease of cattle or goats. the remaining cultures were obtained from a variety of animals and included the wood pigeon bacillus. rabbit antisera were raised to some of the strains and these, together with antisera to m. avium and m. intracellulare, were used to examine sonicate preparations of all the culture ... | 1986 | 3096932 |
| evidence of paratuberculosis in ohio's white-tailed deer, as determined by an enzyme-linked immunosorbent assay. | an enzyme-linked immunosorbent assay (elisa) was developed and used to detect antibodies to mycobacterium paratuberculosis in serum samples obtained in december of 1983 from 954 hunter-killed white-tailed deer (odocoileus virginianus) in 13 ohio counties. positive or negative status was determined by calculating a signal-to-noise ratio, a ratio between the optical density of the test serum and negative reference sera; a ratio of greater than or equal to 3.0 was considered positive. twenty-four s ... | 1986 | 3099612 |
| evaluation of the serological response of sheep in one flock to mycobacterium paratuberculosis by crossed immunoelectrophoresis. | sera from 74 sheep culled from one flock on the basis of performance and response to immunological tests for paratuberculosis or maedi visna were used to evaluate the serological response to a sonicated antigen of mycobacterium paratuberculosis by crossed immunoelectrophoresis. a total of seven precipitating components was demonstrated. four components (a,c,v,w) were detected in low frequency only with sera from animals with paratuberculosis while two components (x,y) were detected in high frequ ... | 1988 | 3130975 |
| identification and control of paratuberculosis in a large goat herd. | mycobacterium paratuberculosis infection was detected in 2 goats in 1974 and in 5 goats in 1975; 5 of which were from a single herd. the magnitude of the subsequent epizootic in the goat herd was not recognized until 1977, when results of bacteriologic culture of fecal and tissue specimens, antibody determinations (agar-gel immuno-diffusion test), and histopathologic studies became available. by 1984, paratuberculosis had been diagnosed in 124 goats. nearly all the goats were being used in antis ... | 1988 | 3150253 |