Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| antibacterial activity of dental implant metals. | this study was conducted to investigate the specific in vitro antibacterial effect of seven dental implant metals on oral bacteria which have often been identified in failing implants. the metals chosen for evaluation were titanium, chromium, cobalt, aluminum, iron, gold, and vanadium. these metals were selected because they are contained in many commonly used dental implants. the bacteria selected for this study included isolates of porphyromonas endodontalis, porphyromonas gingivalis, prevotel ... | 1992 | 1288798 |
| [binding of actinomyces viscosus to collagen]. | the ability of collagen adsorbed on apatitic surfaces to promote adhesion of a. viscosus was studied. treatment of hydroxyapatite (ha) with human type i or type iii collagen strongly promoted adhesion of a. viscosus ly7. tem observations revealed that a. viscosus also attached to fibrils prepared from human type i collagen. the alpha(2) polypeptide derived from type i collagen exhibited moderate activity in promoting binding. mutans of a. viscosus which possess type i fimbriae, but not type 2 fi ... | 1992 | 1303830 |
| humoral igg antibodies to oral microbiota in a population at risk for root-surface caries. | mutans streptococci have been strongly implicated in the initiation of dental caries on coronal surfaces. their role in development of root-surface caries is less clear. the etiologic agents of both types of dental caries are likely to elicit systemic immune responses. the objective of the present study, therefore, was to study the association of clinical variables of disease with humoral igg antibodies to nine oral micro-organisms in 314 adult subjects, aged 45-65 years, who were at risk for ro ... | 1992 | 1321176 |
| inhibition of actinomyces viscosus--porphyromonas gingivalis coadhesion by trypsin and other proteins. | protease activity is associated with the coadhesion of actinomyces viscosus and porphyromonas gingivalis. to try to distinguish whether the recognition/adhesion or degradative functions of proteases are more crucial for coadhesion, we determined the effect of trypsin and other purchased proteases and proteins on coadhesion when they were incorporated in the coadhesion assay buffer or when a. viscosus cells were pretreated with trypsin. coadhesion was measured by the decrease in turbidity caused ... | 1992 | 1328996 |
| role of porphyromonas gingivalis 40-kda outer membrane protein in the aggregation of p. gingivalis vesicles and actinomyces viscosus. | porphyromonas gingivalis, an important pathogen in periodontitis, produces extracellular vesicles that aggregate with actinomyces viscosus cells. a 40-kda outer membrane protein (omp)-coding gene from p. gingivalis was cloned and the protein was found to be localized in these vesicles. the recombinant 40-kda omp did not show aggregation activity. however, affinity-purified antibody against the recombinant protein significantly inhibited aggregation of p. gingivalis vesicles with a. viscosus cell ... | 1992 | 1329700 |
| conservation of an actinomyces viscosus t14v type 1 fimbrial subunit homolog among divergent groups of actinomyces spp. | the type 1 fimbrial subunit gene of the human actinomyces viscosus t14v was used as a dna probe in southern analyses to detect related dna sequences in 16 of 30 strains of actinomyces spp. under conditions of high stringency. the organisms with homology to the dna probe included two human and six nonhuman a. viscosus, three human and three nonhuman a. naeslundii, and two a. bovis isolates. homologous dna sequences were not detected in strains of a. odontolyticus and a. israelii examined in this ... | 1992 | 1347285 |
| isolation and characterization of actinomyces viscosus mutants defective in binding salivary proline-rich proteins. | recent studies have provided evidence for human salivary proline-rich proteins (prps) serving as potential receptors in the acquired pellicle for actinomyces viscosus type 1 fimbriae. we report here the isolation of mutants derived from a. viscosus t14v-j1 which are defective in binding to prps partially purified from parotid gland saliva. mutagenesis with ethyl methanesulfonate preceded enrichment for cells nonreactive with prps by successive adsorptions with prp-treated latex beads. screening ... | 1992 | 1347286 |
| reduction in adherence of actinomyces viscosus after exposure to low-frequency acoustic energy. | the ability of low-frequency (200 hz) acoustic energy to reduce the adherence of actinomyces viscosus t14v to saliva-treated hydroxyapatite (sha) disks was studied. an acoustic pressure range between 0 and 65 kpa and exposure durations between 0 and 8 min were used to study the levels necessary to significantly alter adherence. the effects of acoustic exposure on both bacteria in liquid and bacteria already adhering to sha disks were studied. a modified enzyme-linked immunosorbent assay was used ... | 1992 | 1357620 |
| use of a replica-plate assay for the rapid assessment of salivary protein-bacteria interactions. | a replica-plate assay was used to screen for the interaction of salivary molecules with dental plaque bacteria. bacterial colonies cultured from supragingival plaque on sheep-blood (sb) agar were replica-plated onto nitrocellulose membranes overlaying sb or mitis-salivarius agar. membranes with attached colonies were removed and incubated with 125i-amylase or 125i-proline-rich glycoprotein (prg). positive interactions were detected by autoradiography. only strains of streptococcus gordonii and a ... | 1992 | 1382259 |
| an augmented regenerative technique for severe osseous defects. | techniques for new attachment using the principle of guided tissue regeneration with barrier membranes have become accepted as a method for treating teeth with severe osseous defects and furcation involvement. after an evaluation of existing membranes and techniques, a procedure was developed using an expanded polytetrafluoroethylene soft tissue patch, 1 mm thick (w. l. gore and assoc., flagstaff, az), in conjunction with an alloplast hydroxylapatite bone substitute (ha-500, 40-60 mesh) (orthoma ... | 1992 | 1392503 |
| acid production by actinomyces viscosus of root surface caries and non-caries origin during glycogen synthesis and degradation at different ph levels. | actinomyces viscosus strains, freshly isolated from root surface caries lesions and intact root surfaces, were studied for their glycogen synthetic and degradative activities at ph 4.5, 5.0, and 7.0 in a ph-stat. at all three ph levels, root caries origin of a. viscosus synthesized up to three times as much glycogen compared to non-root caries origin. since root caries origin of a. viscosus strains initially synthesized large amounts of glycogen, a longer period of time was required to deplete t ... | 1992 | 1403842 |
| stimulatory effect of bicarbonate on the glycolysis of actinomyces viscosus and its biochemical mechanism. | the effects of bicarbonate on acid production by 4 human strains of actinomyces viscosus were estimated under anaerobic conditions. the rate of acid production was accelerated by bicarbonate 3-4 times as much as that without bicarbonate. the analyses of intracellular glycolytic intermediates, nad and nadh revealed a decrease in nadh:nad ratio and an increase in the level of 3-phosphoglycerate in the cells when bicarbonate was present. furthermore, when bicarbonate was available, malate dehydroge ... | 1992 | 1408353 |
| effect of slow-release chlorhexidine mouthguards on the levels of selected salivary bacteria. | this study evaluated the effect of an alternate delivery system for chlorhexidine on salivary levels of mutans streptococci (ms) and other selected oral bacteria. on the basis of salivary ms levels > or = 10(4) cfu/ml, 22 subjects were enrolled. all caries lesions were restored prior to treatment. two pretreatment paraffin-stimulated saliva samples were obtained for culturing at least 1 week apart. complete-arch vacuum-adapted mouthguards were individually fabricated and coated internally with a ... | 1992 | 1423441 |
| microbiota associated with residual clefts and neighboring teeth in patients with cleft lip, alveolus, and palate. | twenty patients with residual clefts or pronounced soft tissue grooves, treated for uni- or bilateral cleft lip, alveolus, and palate were included in this study. ten patients were recalled for dental prophylaxis at regular intervals, 10 patients were not. one microbiologic sample was obtained from the cleft area and two samples from a tooth adjacent to the cleft (sites adjacent and distant to the cleft). between the recall and the nonrecall group there were notable differences in the presence o ... | 1992 | 1472527 |
| effects of chlorhexidine-containing gel and varnish on abutment teeth in patients with overdentures. | previous experiments have shown that daily use of 1% chlorhexidine gel strongly reduced caries development in the abutment teeth under overdentures. the present experiment was designed to determine whether intermittent application of chlorhexidine, either in a varnish or in a gel, might be used as an alternative preventive method, less demanding for the patient. to this end, 31 patients were distributed among four treatment groups, with the number of mutans streptococci in plaque and saliva used ... | 1992 | 1522289 |
| multibacterial artificial plaque. a model for studying carious process. | a multibacterial artificial plaque was established in vitro on enamel slabs incubated in mixed culture of streptococcus sanguis, streptococcus mutans (serotype c), streptococcus salivarius, lactobacillus casei and actinomyces viscosus. typical structure same as natural plaque was seen under microscope. the caries-like lesion produced under the plaque was observed in enamel sections. the lesion was similar in appearance to natural ones under optic and scanning electron microscopes. the acids prod ... | 1992 | 1576866 |
| effect of initial treatment of chronic inflammatory periodontal disease on the frequency of peripheral blood t-lymphocytes specific to periodontopathic bacteria. | limit dilution analysis (lda) was used to determine the effect of initial treatment of chronic inflammatory periodontal disease on the frequency of periodontopathic bacteria-specific t-cells in peripheral blood. eleven marginal gingivitis (mg) and 8 adult periodontitis (ap) subjects took part in the study. the proliferative t-lymphocyte precursor (ptl-p) frequencies to porphyromonas gingivalis and actinomyces viscosus were determined using lda and poisson statistics both before and after treatme ... | 1991 | 1667435 |
| dominant low responsiveness in the igg response of mice to the complex protein antigen type 1 fimbriae from actinomyces viscosus t14v. | type 1 fimbriae from actinomyces viscosus t14v, composed of a complex protein of mr 65,000, mediate the adherence of a. viscosus t14v to the host, whereas type 1 fimbriae-specific antibodies inhibit adherence. genetic control of the serum igg response to type 1 fimbriae was evaluated in a series of inbred, hybrid, recombinant inbred, and back-cross mice. mice were given i.p. injections of 10(8) a. viscosus t14v cells in saline on days 0 and 14, and igg anti-type 1 fimbriae in sera obtained on da ... | 1991 | 1672336 |
| genetic control of serum antibody responses of inbred mice to type 1 and type 2 fimbriae from actinomyces viscosus t14v. | antibodies reactive with type 1 and type 2 fimbriae from actinomyces viscosus t14v specifically inhibit the adherence of a. viscosus t14v to salivary pellicle-coated tooth surfaces and other bacteria, and these antibodies are thought to modulate colonization by this microorganism. these studies were done to determine whether previously noted differences in the antibody responses of inbred mice to type 1 and type 2 fimbriae might be under genetic control. the serum immunoglobulin g (igg) and igm ... | 1991 | 1675628 |
| evidence that porphyromonas (bacteroides) gingivalis fimbriae function in adhesion to actinomyces viscosus. | porphyromonas (bacteroides) gingivalis adheres to gram-positive bacteria, such as actinomyces viscosus, when colonizing the tooth surface. however, little is known of the adhesins responsible for this interaction. a series of experiments were performed to determine whether p. gingivalis fimbriae function in its coadhesion with a. viscosus. fimbriae typical of p. gingivalis were isolated from strain 2561 (atcc 33277) by the method of yoshimura et al. (f. yoshimura, k. takahashi, y. nodasaka, and ... | 1991 | 1679428 |
| inhibitory effect of stannous fluoride and other commonly used antimicrobial agents on oral bacteria. | the purposes of this investigation were to evaluate and compare the antimicrobial effect of stannous fluoride (snf2) gel and other commonly used oral antimicrobial agents/products. the antibacterial inhibitory effect of the various agents was evaluated by their effect against oral plaque bacteria including strains of streptococcus sanguis, streptococcus sobrinus, actinomyces viscosus, actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. a lawn of the specific ... | 1991 | 1681003 |
| binding of actinomyces viscosus to collagen: association with the type 1 fimbrial adhesin. | treatment of hydroxyapatite (ha) with human type i or type iii collagen strongly promoted adhesion of actinomyces viscosus ly7 cells. treatment with human type v collagen was somewhat less effective while treatment with human type iv or rat type i collagen was significantly less effective. electron microscopic observations revealed that a. viscosus cells also attached to fibrils prepared from human type i collagen. the alpha 1 (1) polypeptide chain derived from type i collagen was ineffective in ... | 1991 | 1682868 |
| immunochemical and functional studies of actinomyces viscosus t14v type 1 fimbriae with monoclonal and polyclonal antibodies directed against the fimbrial subunit. | each of five monoclonal antibodies (mabs) prepared against the type 1 fimbriae of actinomyces viscosus t14v reacted with a 54 kda cloned protein previously identified as a fimbrial subunit. this purified protein completely inhibited the reaction of a specific anti-type-1-fimbria rabbit antibody with a. viscosus whole cells. maximum values for the number of antibody molecules bound per bacterial cell ranged from 7 x 10(3) to 1.2 x 10(4) for the different 125i-labelled mabs and was approximately 7 ... | 1991 | 1683401 |
| mechanism of gram variability in select bacteria. | gram stains were performed on strains of actinomyces bovis, actinomyces viscosus, arthrobacter globiformis, bacillus brevis, butyrivibrio fibrisolvens, clostridium tetani, clostridium thermosaccharolyticum, corynebacterium parvum, mycobacterium phlei, and propionibacterium acnes, using a modified gram regimen that allowed the staining process to be observed by electron microscopy (j. a. davies, g. k. anderson, t. j. beveridge, and h. c. clark, j. bacteriol. 156:837-845, 1983). furthermore, since ... | 1990 | 1689718 |
| glucanase-producing organisms in human dental plaques. | selective media were used to isolate a wide range of bacteria from sixty human dental plaques. glucanase activities of the isolates were determined on dextran- and starch-containing media. all sixty samples of dental plaque yielded some colonies showing amylolytic and dextranolytic activities. the glucanase-producing organisms comprised 20% of the isolates. of these 38% were gram-positive rods, 27% gram-positive cocci, 28% gram-negative rods and 7% were gram-negative cocci. the cultural groups m ... | 1990 | 1691432 |
| complement activation by individual and combinations of monoclonal antibodies to actinomyces viscosus t14v fimbriae: a probe for epitope distribution on these polymeric proteins. | the spatial requirements for igg activation of the classical complement pathway has provided a basis for utilizing complement consumption by individual and pairs of monoclonal antibodies (mabs) to compare the repeating epitope patterns of the type 1 and type 2 fimbriae of actinomyces viscosus t14v and to examine the co-operative effects of mabs against these polymeric proteins. three of five mabs specific for the type 1 fimbriae consumed complement when assayed individually. four patterns of com ... | 1990 | 1698258 |
| cariogenicity of milchschnitte (ferrero gmbh) and apple in program-fed rats. | the cariogenicity of ferrero milchschnitte and apple was tested in rats using a frequency-controlled feeding machine. twelve litters of osborne-mendel rats orally infected with streptococcus sobrinus omz-176 and actinomyces viscosus ny-1 were used. four treatment groups were tested: (1) cooked starch/sucrose 1:1 as positive control; (2) ferrero milchschnitte; (3) apple; (4) gastric intubation only as negative control. groups 1-3 were additionally fed by gastric intubation with essential nutrient ... | 1991 | 1747885 |
| isolation of a neuraminidase gene from actinomyces viscosus t14v. | a genomic library of actinomyces viscosus t14v dna in lambda gt11 was screened for expression of neuraminidase activities. four recombinant clones were detected that gave blue fluorescence upon incubation with a fluorogenic substrate, 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid. of these, two were identical, and all of the neuraminidase-positive clones shared a common 3.4-kbp dna region. expression of the enzyme activities in escherichia coli carrying the cloned dna was independent ... | 1991 | 1781674 |
| cloning, sequencing and expression of the sialidase gene from actinomyces viscosus dsm 43798. | chromosomal dna from actinomyces viscosus was digested with restriction endonucleases and the fragments ligated with puc-vectors were used to transform escherichia coli cells. clones bearing the required sialidase gene were detected by spraying the colonies with the fluorogenic sialidase substrate mu-neu5ac. the identity of the cloned sialidase was confirmed after the 5700-fold enrichment and comparison with the purified enzyme of a. viscosus. both sialidases were identical with regard to molecu ... | 1991 | 1789931 |
| tarnish of dental alloys by oral microorganisms. | five dental alloys, on exposure to blood and chocolate media with and without inoculated microorganisms, showed varying degrees of tarnish. the results indicated a composition-dependent tarnish behavior of alloys in microorganism-inoculated media, indicating a potential role for the oral microorganisms in inducing clinically observed tarnish of dental alloys. actinomyces viscosus and periodontal pocket specimens show a similarity in their activity to induce tarnish in base metal-containing denta ... | 1991 | 1805014 |
| characterization of unusual tetracycline-resistant gram-positive bacteria. | tetracycline-resistant tet m-negative isolates of actinomyces viscosus, eubacterium lentum, mobiluncus curtisii, and mobiluncus mulieris were screened with the tet k, tet l, and tet o dna probes. ten (71%) of the resistant mobiluncus strains hybridized with the tet o probe, two of the three e. lentum strains hybridized with the tet k probe, and the a. viscosus isolate hybridized with the tet l probe. | 1991 | 1810204 |
| association of proteases of porphyromonas (bacteroides) gingivalis with its adhesion to actinomyces viscosus. | p. gingivalis adheres to a. viscosus on mineral surfaces mimicking teeth. to study whether p. gingivalis proteases contribute to its binding, mutants of p. gingivalis deficient in proteases were compared with their parent strain and a p. gingivalis-type strain for their adherence to a. viscosus on saliva-coated hydroxyapatite by manipulating a radio-isotope binding assay. adherence of p. gingivalis 2561 to a. viscosus was studied by tests of the effects of incubation temperature and known inhibi ... | 1991 | 1846887 |
| root surface caries prevalence and associated factors among adult patients in an acute care hospital. | there have been many studies reporting on the prevalence of root surface caries in elderly populations, but there is little consistency in these reports concerning the associated risk factors. in the present study, intraoral examinations were carried out on adult patients (age range 22 to 91 years, mean age 57.9) on the acute care wards of a teaching hospital. of the 103 patients examined, 23.3% exhibited carious lesions on root surfaces, these lesions occurring more frequently in males than in ... | 1991 | 1860090 |
| effects of fluoride, lithium, and strontium on growth and acid production of mutants streptococci and actinomyces viscosus. | eight strains of mutants streptococci and two strains of actinomyces viscosus were studied to determine (1) their relative sensitivities to fluoride, lithium, and strontium and (2) whether lithium or strontium interact to enhance the known antimicrobial effects of fluoride. analyses of variance of the data revealed a major inhibition of growth and acid production by fluoride, but not by lithium or strontium. fluoride (5 mg/l) inhibited cell growth of all strains tested by a mean of 17% and total ... | 1991 | 1878915 |
| interactions of sanguinarine and zinc on oral streptococci and actinomyces species. | sanguinaria extract, which contains benzophenanthridine alkaloids, has been used as a folk medicine for many years. minimum inhibitory and minimum bactericidal concentrations (mic and mbc values) for sanguinarine were determined for common and etiologically important plaque bacteria. because the efficacy of sanguinarine is believed to be enhanced by zinc, isobolograms were assessed to determine their mode(s) of interaction. hydrogen ion concentration influenced the inhibitory activity of both sa ... | 1991 | 1878916 |
| complete structure of the cell surface polysaccharide of streptococcus oralis c104: a 600-mhz nmr study. | specific lectin-carbohydrate interactions between certain oral streptococci and actinomyces contribute to the microbial colonization of teeth. the receptor molecules of streptococcus oralis, 34, atcc 10557, and streptococcus mitis j22 for the galactose and n-acetylgalactosamine reactive fimbrial lectins of actinomyces viscosus and actinomyces naeslundii are antigenically distinct polysaccharides, each formed by a different phosphodiester-linked oligosaccharide repeating unit. these streptococci ... | 1991 | 1888724 |
| the comparative antimicrobial effect of calcium hydroxide. | the antimicrobial effectiveness of calcium hydroxide, camphorated paramonochlorophenol, and formocresol in root canals of extracted human teeth was compared. canals in single-rooted teeth were enlarged and inoculated with streptococcus mutans, actinomyces viscosus, and bacteroides gingivalis or bacteroides fragilis. after treatment with a test agent and sealing and incubation for 1 hour, the canal contents were analyzed for the number of viable test bacteria and compared with that of inoculated ... | 1991 | 1891227 |
| salivary-agglutinin-mediated adherence of streptococcus mutans to early plaque bacteria. | interspecies binding is important in the colonization of the oral cavity by bacteria. streptococcus mutans can adhere to other plaque bacteria, such as streptococcus sanguis and actinomyces viscosus, and this adherence is enhanced by saliva. the salivary and bacterial molecules that mediate this interaction were investigated. salivary agglutinin, a mucinlike glycoprotein known to mediate the aggregation of many oral streptococci in vitro, was found to mediate the adherence of s. mutans to s. san ... | 1991 | 1894355 |
| adhesion of actinomyces viscosus to porphyromonas (bacteroides) gingivalis-coated hexadecane droplets. | interbacterial adhesion (coadhesion) is considered a major determinant of dental plaque ecology. in this report, we studied several aspects of the adhesion of porphyromonas (bacteroides) gingivalis to hexadecane in order to use the liquid hydrocarbon as a convenient substratum for coadhesion assays. washed suspensions of hydrophobic p. gingivalis 2561 cells were vortexed with hexadecane to yield highly stable cell-coated droplets. kinetics of coadhesion between actinomyces viscosus cells and p. ... | 1991 | 1901571 |
| effect of calcium ions on cell surface electrostatics of bacteroides gingivalis and other oral bacteria. | surface electrostatics of bacteroides gingivalis and other oral bacteria were examined. a polarization circuit was employed using platinum electrodes exposed in each bacterial suspension and the number of bacteria adsorbed to the anode and cathode were then estimated. in all bacteria (b. gingivalis, streptococcus sobrinus, s. mutans, s. salivarius, s. sanguis and actinomyces viscosus), the number of cells adsorbed to the anode were much greater than the number of cells adsorbed to the cathode. t ... | 1991 | 1930564 |
| reduced bactericidal activity in neutrophils from scorbutic animals and the effect of ascorbic acid on these target bacteria in vivo and in vitro. | actinomycetes, involved in oral and periodontal diseases, cause serious infections in immunocompromised hosts. severely scorbutic guinea pig leukocytes killed only 12% of phagocytosed actinomycetes, had distorted nuclear morphology, had 16 times less ascorbate, and had no chemotactic responses in vitro. ascorbate reversed these indices and also prevented nitrosamine formation by oral organisms. degranulating leukocytes release lactoferrin and ascorbate that chelate iron, essential for microorgan ... | 1991 | 1962573 |
| [studies on immunobiological activities of periodontopathic bacteria. comparison of the activities of soluble components from bacteroides gingivalis and actinomyces viscosus]. | it has been proposed that bacteroides gingivalis and actinomyces viscosus are most important agents to pathogenesis of the periodontitis and gingivitis. in this study, the influences of sonic extracts prepared from b. gingivalis and a. viscosus for dna synthesis of murine lymphocytes, production of prostaglandin e2 (pge2), collagenase and interleukin-1 (il-1) from human peripheral monocyte were investigated. furthermore, pge2 and collagenase production by fibroblasts from human periodontal ligam ... | 1990 | 1966895 |
| sequence homology between the subunits of two immunologically and functionally distinct types of fimbriae of actinomyces spp. | nucleotide sequencing of the type 1 fimbrial subunit gene of actinomyces viscosus t14v revealed a consensus ribosome-binding site followed by an open reading frame of 1,599 nucleotides. the encoded protein of 533 amino acids (mr = 56,899) was predominantly hydrophilic except for an amino-terminal signal peptide and a carboxy-terminal region identified as a potential membrane-spanning segment. edman degradation of the cloned protein expressed in escherichia coli and the type 1 fimbriae of a. visc ... | 1990 | 1970561 |
| binding of colloidal gold-labeled salivary proline-rich proteins to actinomyces viscosus type 1 fimbriae. | salivary proline-rich proteins (prps), which were purified from parotid saliva, were adsorbed onto 15-nm-diameter gold particles to visualize specific binding of the salivary molecules to actinomyces viscosus type 1 fimbriae. negatively stained preparations incubated with prp-gold conjugates but not bovine serum albumin-gold complexes bound specifically to bacteria possessing type 1 fimbriae, a. viscosus t14v-j1 and 5519. binding of the prp-gold probes to strains deficient in type 1 fimbriae, i. ... | 1990 | 1971260 |
| cooperative complement- and bacterial lectin-initiated bactericidal activity of polymorphonuclear leukocytes. | the recognition of glycoconjugate receptors on sialidase-treated polymorphonuclear leukocytes (pmns) by the gal/galnac-reactive fimbrial lectin of actinomyces viscosus t14v has previously been shown to initiate lactose-inhibitable phagocytosis and subsequent killing of the bacteria. although a mutant lacking fimbriae, a. viscosus 147, was not destroyed by this mechanism, the present studies demonstrate that the deposition of c3 fragments on this bacterium by anti-a. viscosus 147 immunoglobulin m ... | 1991 | 1987035 |
| effects of fluoride and chlorhexidine on the microflora of dental root surfaces and progression of root-surface caries. | the effects of fluoride and chlorhexidine varnishes on the microflora of dental root surfaces and on the progression of root-surface caries were studied. forty-four patients, surgically treated for advanced periodontal disease, were distributed at random among three groups. all patients received a standardized preventive treatment. furthermore, the dentition of the patients in the two experimental groups was treated, at three-month intervals, with chlorhexidine and fluoride varnish, respectively ... | 1991 | 1991872 |
| microbiological and immunological aspects of experimental periodontal disease in rats: a review article. | animal models in which microbiological and immunological aspects of periodontal disease can be studied prospectively seem well warranted. the rat bears much resemblance to man with respect to periodontal anatomy, development and composition of dental plaque, histopathology of periodontal lesions, and basic immunobiology. furthermore, reproducible methods are available for assessment of periodontal disease in rats, and detectable periodontal destruction can be induced in a few weeks in these anim ... | 1991 | 2002433 |
| effects of chlorhexidine on proteolytic and glycosidic enzyme activities of dental plaque bacteria. | chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by treponema denticola, porphyromonas gingivalis, bacteroides intermedius, actinobacillus actinomycemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga orchracea, capnocytophaga sp., actinomyces viscosus, streptococcus mitior, streptococcus mutans, streptococcus sobrinus, streptococcus mitis, streptococcus anginosus, streptococcus oralis and strepto ... | 1991 | 2005230 |
| skin pustules and nodules caused by actinomyces viscosus in a horse. | a 12-year-old arabian stallion was evaluated for a refractory skin problem of 2 years' duration. skin nodules and pustules, which would rupture, had developed over the right lumbar muscles. anaerobic culturing from the pustules yielded actinomyces viscosus, and histologic examination of biopsy specimens revealed globular eosinophilic structures. concomitant treatment with isoniazid (8 mg/kg of body weight, q 24 h for 8 weeks), trimethoprim-sulfadiazine (30 mg/kg, q 24 h for 8 weeks), and sodium ... | 1991 | 2010343 |
| antimicrobial and anti-plaque activity of n'-alkyl-n-(2-aminoethyl)piperidine against dental plaque bacteria. | five n'-alkyl-n-(2-aminoethyl)piperidines were synthesized and their in vitro antimicrobial activities were tested against four micro-organisms related to dental caries (streptococcus mutans, s. sobrinus, actinomyces viscosus, and a. naeslundii) which are known to be implicated in dental caries. the tetradecyl and hexadecyl derivatives possessed good bacteriostatic activity. some derivatives exhibited a rapid bactericidal effect against s. mutans and s. sobrinus in aqueous solution. these compou ... | 1991 | 2013844 |
| complete structure of the cell surface polysaccharide of streptococcus oralis atcc 10557: a receptor for lectin-mediated interbacterial adherence. | lectin-carbohydrate binding is known to play an important role in a number of different cell-cell interactions including those between certain species of oral streptococci and actinomyces that colonize teeth. the cell wall polysaccharides of streptococcus oralis atcc 10557, s. oralis 34, and streptococcus mitis j22, although not identical antigenically, each function as a receptor molecule for the galactose and n-acetylgalactosamine reactive fimbrial lectins of actinomyces viscosus and actinomyc ... | 1991 | 2054351 |
| effect of nutrients on defined bacterial plaques and streptococcus mutans c67-1 implantation in a model mouth. | actinomyces viscosus wvu 627, streptococcus oralis lpa-1 and veillonella dispar omz 193 were cocultured on teeth in a model mouth for 66 h. synthetic saliva containing bovine salivary glycoprotein supported bacterial growth, although the delivery of an intermittent nutrient supplement, containing 1% (w/v) glucose or sucrose, gave greater bacterial cell and viable counts. when streptococcus mutans c67-1 was super-inoculated onto 24-hour mixed plaques, it became established under all regimens, but ... | 1991 | 2059972 |
| oxygen-dependent lactate utilization by actinomyces viscosus and actinomyces naeslundii. | actinomyces viscosus and actinomyces naeslundii were grown in chemostat cultures with glucose or lactate as the sole sources of energy. growth on lactate was dependent on oxygen. lactate was oxidised partly to acetic acid. as judged from the low carbon recoveries in acidic degradation products, it is concluded that a substantial part of the lactate was oxidised to carbon dioxide. assuming that 1 atp is generated in the transfer of an electron pair to oxygen, yatp values of 10-11 were calculated ... | 1990 | 2082247 |
| humoral immunity in root caries in an elderly population. 1. | iga, igg and igm antibody activity (elisa units/ml) to streptococcus mutans, actinomyces viscous and escherichia coli cf8 in serum, parotid saliva and whole saliva was measured using the amplified elisa (a-elisa) while the concentration (microgram/ml) of each isotype of immunoglobulin as well as albumin and lactoferrin, was determined using sandwich elisas. selection of suitable reagents from those commercially available was based on specificity tests using purified human immunoglobulin; most po ... | 1990 | 2087357 |
| role of cryptic receptors (cryptitopes) in bacterial adhesion to oral surfaces. | progress in characterizing the receptors that promote bacterial attachment to teeth and oral epithelial cells has suggested that hidden molecular segments may frequently be involved. such cryptic receptors, referred to as 'cryptitopes', may become exposed by several mechanisms. hidden segments of salivary acidic proline-rich proteins evidently become exposed when the molecules undergo a conformational change as they adsorb to apatitic mineral. adhesins of actinomyces viscosus and certain other p ... | 1990 | 2088213 |
| bactericidal effects of human neutrophils and sera on selected endodontic pathogenic bacteria in an anaerobic environment. | phagocytosis by normal human neutrophils and the bactericidal activity of normal human pooled serum were measured under anaerobic conditions on six endodontic pathogenic bacterial species from the genera actinomyces, bacteroides, fusobacterium, peptostreptococcus and streptococcus. the results revealed that actinomyces viscosus was not killed either by human neutrophils or by 20 per cent serum; peptostreptococcus anaerobius was not killed by either 20 per cent or 95 per cent serum; and bacteroid ... | 1990 | 2098335 |
| penetration of dentine by three oral bacteria in vitro and their associated cytotoxicity. | three common oral bacteria, namely streptococcus sanguis, actinomyces viscosus and corynebacterium spp. were studied with regard to their ability to penetrate etched and unetched dentine and for their effect on underlying cell cultures. the test organisms were grown in cylinders above dentine slices 100 and 500 microns thick for 72 hours. the slices were in contact with tissue culture medium covering a layer of fibroblasts. penetration of 100 microns slices was most rapid with s. sanguis, follow ... | 1990 | 2098336 |
| ph-dependent fluoride inhibition of catalase activity. | the inhibitory effects of fluoride on several catalases were examined over a range of ph conditions. preparations of bovine-liver catalase were sensitive to fluoride under acidic conditions. catalase activity associated with whole-cell preparations of actinomyces viscosus np 311a remained relatively constant between ph 3.0 and 8.0 and was inhibited by fluoride in a ph-dependent manner. fluoride was also observed to enhance hydrogen peroxide killing of a. viscosus np 311a under acidic ph conditio ... | 1990 | 2098711 |
| antimicrobial activities of thiolactomycin against gram-negative anaerobes associated with periodontal disease. f1. | thiolactomycin (tlm), (4r)-(2e,5e)-2,4,6-trimethyl-3-hydroxy-2,5, 7-octatriene-4-thiolide, purified from a culture filtrate of a strain of the nocardia species, was examined for antimicrobial activities against more than 100 strains of oral and periodontally associated bacteria. nine other commonly used antibiotics were also included for the test. we found that tlm exhibited strong and selective antimicrobial activities against bacteroides gingivalis and other oral black-pigmented bacteroides sp ... | 1990 | 2098714 |
| association of selected bacteria with the lesions of root surface caries. | plaque from the root surfaces of 165 subjects (mean age 65.5 years, 22-26 teeth/subject) was analysed for specific bacteria. five subject groups were defined: a (dmfs 16.4), b (dmfs 55.9), c1 (dmfs 55.6), c2 (dmfs 57.0) and c3 (dmfs 48.1). groups c1 and c2 had unrestored root surface lesions; group a, b and c3 were free of unrestored root caries and differed in their coronal caries experience. streptococcus mutans was isolated more frequently from the root lesions in groups c1 and c2 than from i ... | 1990 | 2098715 |
| studies of the subgingival microflora in patients with acquired immunodeficiency syndrome. | two unique forms of periodontal disease, hiv-gingivitis and hiv-periodontitis, have been described in patients with acquired immunodeficiency syndrome (aids). in order to determine the bacterial species associated with periodontitis in aids patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 aids patients with periodontitis. the presence of putative periodontal pathogens including actinobacillus actinomycetemcomitans, bacteroides intermedius, por ... | 1990 | 2123926 |
| influence of sweetening agents in solution on dental caries in desalivated rats. | sucralose (trichlorogalactosucrose), sorbitol and aspartame in drinking water induced little or no caries in desalivated rats infected with streptococcus sobrinus and actinomyces viscosus and receiving their essential nutrition by gastric gavage. in contrast, sucrose and fructose induced extensive decay. only sucrose could sustain implantation of strep. sobrinus in these animals. the populations of a. viscosus were sparse (0.3-0.5%) in the animals given fructose and sucrose. large populations of ... | 1990 | 2124801 |
| [non-cariogenicity of erythritol as a substrate]. | erythritol is a sugar alcohol which is obtained through a cultivation of glucose and aureobasidium sp. the sugar is about 70-80% as sweet as sucrose and is also non-hygroscopic. the effect of erythritol on cariogenicities of mutans streptococci (serotype a-h) and certain oral microorganisms was studies. erythritol was not utilized as a substrate for the growth, lactic acid production and plaque formation of mutans streptococci (serotype a-h). it did not serve as a substrate for cellular aggregat ... | 1990 | 2134936 |
| [lysis of the cells of propionibacterium acnes by the culture supernatant of actinomyces viscosus]. | ecologically there seemed to be antagonistic relations between actinomyces viscosus and propionibacterium acnes. the supernatant of a. viscosus was shown to possess a lytic activity on the growing cells of p. acnes. the results obtained were as follows: 1. only a small number of p. acnes was isolated in samples from which numerous a. viscosus were isolated. on the other hand, p. acnes were frequently isolated in samples from which few cells of a. viscosus were isolated. 2. the concentrate with a ... | 1990 | 2135129 |
| [studies of antigens related to metabolic and growth inhibitions of staphylococcus aureus l-form]. | metabolic and growth inhibiting activities in immunoglobulin (of anti-s. aureus l-form serum and anti-s. aureus coccal form serum) could be absorbed by cell membranes of s. aureus l-form and its coccal form, respectively. these activities could not be absorbed by cell membrane of micrococcus luteus, streptococcus pyogenes or actinomyces viscosus. these findings suggested the existence of species-specific antigens of cell membrane. the membrane antigens of l-form related to the metabolic and grow ... | 1990 | 2135412 |
| selective modulation of bacterial attachment to oral epithelial cells by enzyme activities associated with poor oral hygiene. | the present investigation explored the hypothesis that elevated levels of certain enzymes in the gingival crevicular environment of individuals with poor oral hygiene and/or gingival inflammation may modify the surfaces of epithelial cells and thereby modulate the types of bacteria which attach and colonize. buccal epithelial cells treated with neuraminidase and certain proteases were used as a model for study. bacteria studied included streptococcus sanguis and streptococcus mitis which have be ... | 1990 | 2141877 |
| the influence of sucralose on bacterial metabolism. | sucralose (1',4',6' trideoxy-trichloro-galactosucrose) is a nontoxic, intensely sweet sucrose derivative that has been shown to be non-cariogenic in experimental animals. the purpose of this study was to determine whether certain oral bacteria could utilize sucralose. sucralose, as a sole carbon source, was unable to support growth of ten strains of oral bacteria and dental plaque. when sucrolose was incorporated into a liquid medium containing glucose or sucrose, all organisms tested displayed ... | 1990 | 2143512 |
| microbial changes associated with the development of puberty gingivitis. | in this study, longitudinal changes in the composition of the subgingival microbiota of children between the ages of 11 and 14 and their association with changes of clinical parameters describing gingival health were investigated. during 4 years, subgingival microbial samples were taken in 22 boys and 20 girls 10 times. at the same time the gingival bleeding tendency was recorded by the papillary bleeding index (pbi). a total of 840 samples was evaluated using darkfield microscopy and anaerobic ... | 1990 | 2148945 |
| complete structure of the polysaccharide from streptococcus sanguis j22. | the cell wall polysaccharides of certain oral streptococci such as streptococcus sanguis strains 34 and j22, although immunologically distinct, act as receptors for the fimbrial lectins of actinomyces viscosus t14v. we report the complete covalent structure of the polysaccharide from s. sanguis j22 which is composed of a heptasaccharide subunit linked by phosphodiester bonds. the repeating subunit, which contains alpha-galnac, alpha-rhamnose, beta-rhamnose, beta-glucose, and beta-galactose all i ... | 1990 | 2157479 |
| microbiology of root surface caries in humans. | studies on the microbiology of root surface caries between 1970 and 1975 placed emphasis on gram-positive pleomorphic filamentous rods, particularly actinomyces viscosus and actinomyces naeslundii. both of these species had been shown to produce root surface caries in experimental animals. since this time, studies have placed more emphasis on streptococcus mutans, and s. mutans and lactobacillus are significant in prediction of root surface caries risk in patients. subsequent studies confirmed a ... | 1990 | 2186069 |
| effect of ph on the growth and proteolytic activity of porphyromonas gingivalis and bacteroides intermedius. | the effect of ph on the growth and proteolytic activity of the type strain and fresh isolates of porphyromonas gingivalis and bacteroides intermedius was investigated. b. intermedius strains grew with and without glucose at a ph as low as 5.0. these bacteria grew almost as well as streptococcus mutans at ph 5.0 and better than actinomyces viscosus at ph 5.5 and 5.0. some b. intermedius strains raised the culture ph when grown at a low ph without glucose. in contrast, p. gingivalis strains grew o ... | 1990 | 2191980 |
| aggregation of actinomyces strains by extracellular vesicles produced by bacteroides gingivalis. | the aggregation of actinomyces viscosus and actinomyces naeslundii with extracellular vesicles of bacteroides gingivalis was studied. factors influencing the aggregation phenomenon were examined. l-arginine was found to effectively inhibit aggregation as was an antibody preparation directed against a b. gingivalis surface hemagglutinin. aggregation occurred over a wide ph range and did not seem to be affected by high salt concentrations or the presence of carbohydrates. treatment of the vesicle ... | 1990 | 2202505 |
| [immune response during experimental periodontitis in mice]. | four groups of mice, each of a different genotype, were orally inoculated with actinomyces viscosus. the resulting periodontal bone loss was monitored and the isotype specific anti-a. viscosus serum humoral immune responses measured by elisa. maximum periodontal bone loss occurred in all genotypes with inoculation of 1.6 x 107 colony forming units of a. viscosus. igg and igm isotype levels increased with inoculation of 1.6 x 107 colony forming units of a. viscosus in three of the four groups, in ... | 1990 | 2213541 |
| effect of chlorhexidine gel treatment supplemented with chlorhexidine varnish and resin on mutans streptococci and actinomyces on root surfaces. | the supplementary effect of chlorhexidine varnish and resin on the reappearance of mutans streptococci and actinomyces spp. on root surfaces after chlorhexidine gel treatment was studied. in 8 subjects with many restored tooth and exposed root surfaces, highly colonized with mutans streptococci, chlorhexidine varnish and resin were applied to the teeth on one side of the mouth and resin alone to the other side. this treatment significantly prolonged the time period of reduced salivary levels of ... | 1990 | 2276158 |
| [a case of thoracic actinomyces viscosus infection]. | actinomyces viscosus was isolated from the pleural puncture sample of a 35-year-old patient who was admitted with the complaints of fever, cough and right side chest pain. | 1990 | 2283978 |
| cellular coaggregation of oral streptococcus milleri with actinomyces. | oral isolates of streptococcus milleri were examined for their ability to coaggregate with actinomyces. of the 68 s. milleri strains tested, including 3 reference strains, 40 strains coaggregated with actinomyces naeslundii wvu45 (actinomyces coaggregation group b) and 36 strains coaggregated with actinomyces viscosus t14v (actinomyces coaggregation group a). all s. milleri strains of serotypes b (4 strains), e (2 strains), and f (24 strains) coaggregated with both of the actinomyces. the coaggr ... | 1990 | 2294047 |
| colonization resistance of defined bacterial plaques to streptococcus mutans implantation on teeth in a model mouth. | we investigated the ability of streptococcus mutans c67-1 to colonize simple bacterial plaques and the effects of age and stability of the pre-formed plaque on colonization resistance. mixed-plaques of actinomyces viscosus wvu627, 'streptococcus mitior' lpa-1, and veillonella dispar omz193 were grown on tooth segments, mounted back to back for simulation of approximal sites in a model mouth for 66 h. s. mutans c67-1 was either included in the original inoculum or super-inoculated onto the develo ... | 1990 | 2307751 |
| surface-associated properties of streptococcus milleri group strains and their potential relation to pathogenesis. | thirty strains from the streptococcus milleri (anginosus) group (smg) obtained from various sources were tested for a range of characters that could be associated with pathogenicity and the results were compared with those for type strains of s. sanguis, s. mutans and s. pyogenes. the smg strains were heterogeneous in all tests. most (18) belonged to one of the lancefield groups with group f predominating. adherence of strains isolated from abscesses to buccal epithelial cells was greater than t ... | 1990 | 2325115 |
| microflora associated with experimental root surface caries in humans. | this study describes the microflora from actively progressing root surface caries lesions, in which mineral loss had been determined by quantitative microradiography. the caries lesions were produced experimentally in root surface specimens from human molars inserted in lower partial dentures carried for 3 months by six elderly individuals. a total of 780 bacterial isolates were identified from 13 plaque samples, collected with a punch technique, and six dentin samples. the composition of the mi ... | 1990 | 2341169 |
| adherence of mutans streptococci to other oral bacteria. | adherence of mutans streptococci to strains of actinomyces viscosus, streptococcus sanguis, and streptococcus mitis immobilized on a nitrocellulose membrane was measured. strains of streptococcus mutans, s. sobrinus, and s. rattus bound in a lactose-independent manner to a variety of the actinomyces and streptococci. most of these reactions could proceed in the presence of whole saliva although adherence of s. rattus bht to the streptococci was inhibited by salivary molecules. in contrast, adher ... | 1990 | 2341174 |
| salivary conditions and cariogenic microorganisms in 55, 65, and 75-year-old swedish individuals. | secretion rate, buffer capacity, and cariogenic microorganisms of resting and stimulated whole saliva were examined in 208 55-, 65-, and 75-yr-old swedes. the secretion rate for both resting and stimulated saliva decreased with age. when the use of drugs was taken into account the difference in terms of age group was significant for resting saliva alone (p less than 0.01). in 22% the resting saliva was less than 0.1 ml/min, and in 5% the stimulated saliva was less than 0.7 ml/min. persons with s ... | 1990 | 2349449 |
| characterization of the binding of actinomyces naeslundii (atcc 12104) and actinomyces viscosus (atcc 19246) to glycosphingolipids, using a solid-phase overlay approach. | actinomyces naeslundii (atcc 12104) and actinomyces viscosus (atcc 19246) were radiolabeled externally (125i) or metabolically (35s) and analyzed for their ability to bind glycosphingolipids separated on thin layer chromatograms or coated in microtiter wells. two binding properties were found and characterized in detail. (i) both bacteria showed binding to lactosylceramide (laccer) in a fashion similar to bacteria characterized earlier. the activity of free laccer was dependent on the ceramide s ... | 1990 | 2358461 |
| the effects of sucralose on coronal and root-surface caries. | sucralose is an intensely sweet, chlorinated carbohydrate structurally similar to sucrose; thus, its cariogenic potential is of great interest. four groups of 12 sprague-dawley rats were infected with streptococcus mutans (sobrinus), caged in a könig-höfer programmed feeding machine, and fed diet 2000 or modified diet to contain sucralose at various concentrations instead of sucrose. rats fed sucralose diet developed significantly fewer lesions than did the rats fed regular diet 2000. animals fe ... | 1990 | 2384624 |
| immunochemical studies on levans from several strains of actinomyces viscosus. | high molecular-weight levans elaborated by 8 separate strains of actinomyces viscosus were purified: the inteactions of these levans with concanavalin a and anti-fructan myeloma immunoglobulins upc-10 and j606 were examined by the quantitative precipitin method. oligosaccharides released from the levans by partial acid hydrolysis were separated by partition chromatography on paper and characterized in situ by selective spray reagents. the liberated oligomers were compared with oligomers of known ... | 1990 | 2390024 |
| antimicrobial effect of chlorhexidine in a controlled release delivery system. | chlorhexidine is widely used as a mouth rinse in the prevention and treatment of periodontal diseases and dental caries. the purpose of the present study was to evaluate the in vitro antimicrobial effect of chlorhexidine in a controlled release delivery system. the controlled release dispenser comprised a polymeric inner core matrix containing the medicament with an outer vinyl membrane controlling the drug release. the effect on the following bacteria was studied: actinobacillus actinomycetemco ... | 1990 | 2390965 |
| actinomyces georgiae sp. nov., actinomyces gerencseriae sp. nov., designation of two genospecies of actinomyces naeslundii, and inclusion of a. naeslundii serotypes ii and iii and actinomyces viscosus serotype ii in a. naeslundii genospecies 2. | dnas of type strains and representative members of actinomyces groups from the human periodontal flora and from other habitats were compared by using the s1 nuclease procedure to determine their genetic relatedness. one rather common group from the human periodontal flora, previously called "actinomyces d08," is phenotypically distinct from, and genetically unrelated to, previously described species. we propose the name of actinomyces georgiae for this organism; the type strain is strain atcc 49 ... | 1990 | 2397195 |
| [polyclonal induction of igg antibody-forming cells (afc) by the stimulation of actinomyces viscosus t 14 v]. | 1985 | 2411833 | |
| immunological study of lactate dehydrogenase from streptococcus mutans and evidence of common antigenic domains with lactate dehydrogenases from lactic bacteria. | rabbit polyclonal antibodies directed against purified streptococcus mutans l-(+)-lactate dehydrogenase reacted with the purified enzyme, giving a marked deviation of its kinetic parameters. the enzyme affinity for pyruvate or nadh decreased in the presence of antibody, the affinity for fructose 1,6-diphosphate (fdp) appeared to be slightly affected, and the cooperativity of the ligand binding was lowered. a partial protective effect was observed when the enzyme was preincubated with fdp prior t ... | 1986 | 2416689 |
| identification of bacteria in association with immune components in human carious dentin. | actinomyces viscosus, a. naselundii, streptococcus mutans serotype "c" and s. mutans serotype "d/g" were identified in human carious dentin using histological and immunofluorescent techniques. a. viscosus was most frequently found in association with patient's immunoglobulins and complement, followed by s. mutans serotype "d/g", s. mutans serotype "c", and a. naeslundii. | 1987 | 2443627 |
| membrane atpases and acid tolerance of actinomyces viscosus and lactobacillus casei. | lactobacillus casei atcc 4646 and actinomyces viscosus omz105e were found to differ markedly in acid tolerance. for example, ph profiles for glycolysis of intact cells in dense suspensions indicated that glycolysis by l. casei had an optimal ph of about 6.0 and that glucose degradation was reduced by 50% at a ph of 4.2. comparable values for a. viscosus cells were at phs of about 7.0 and 5.6. the difference in acid tolerance appeared to depend mainly on membrane physiology, and the addition of 4 ... | 1987 | 2445289 |
| the effect of ascorbic acid deficiency on leukocyte phagocytosis and killing of actinomyces viscosus. | the ascorbic acid content of guinea pig leukocytes is reduced by a factor of 16:1 between normal and scorbutic guinea pigs. ascorbic acid deficiencies do not appear to affect phagocytic activity but do change leukocyte morphology. a deficiency of this vitamin appears to significantly interfere with the in vitro bactericidal effectiveness of circulating leukocytes against ingested, cell-associated, and extracellular bacterial cells of the oral pathogen, actinomyces viscosus. leukocytes from scorb ... | 1988 | 2461911 |
| [effect of systemic oral administration of tetracycline on experimental gingivitis in golden hamsters]. | local and systemic administrations of tetracycline have been used in human periodontal treatment for conditions including juvenile periodontitis and rapidly progressive periodontitis, although microbiological effects of the treatment have not been clear. the effect of systemic oral administration of tetracycline on subgingival bacteria in experimental periodontal disease in hamsters as an animal model has not yet been reported. the aim of this study was to investigate changes in subgingival bact ... | 1989 | 2489558 |
| [experiment study of the coexistence and acid producing of streptococcus mutans and actinomyces viscosus]. | 1989 | 2503315 | |
| cariological assessment of leucrose [d-glucopyranosyl-alpha(1----5)-d-fructopyranose] as a sugar substitute. | leucrose [d-glucosyl-alpha(1----5)-d-fructopyranose] prepared by microbial-enzymatic transglycosidation from sucrose, is the first alpha(1----5)-linked disaccharide which possesses excellent nutritional properties with regard to metabolic utilization and is well tolerated. the aim of the present work was to assess its cariogenic potential. yeast invertase was shown to be inhibited by leucrose in a noncompetitive way, while hydrogenated leucrose (leucritol) acted as an activator. plaque polysacch ... | 1989 | 2504493 |
| increased periodontal bone loss in temporarily b lymphocyte-deficient rats. | in order to study the role of t lymphocytes and b lymphocytes in the development of marginal periodontitis, experiments were performed on specific-pathogen-free (spf) rats with various immunologic profiles. the study comprised nude (congenitally t lymphocyte-deficient), thymus-grafted nude (t-lymphocyte reconstituted), anti-mu treated (temporarily b lymphocyte-deficient), and normal rats. when they were 5 to 9 weeks old, some of the rats were inoculated with actinomyces viscosus. bacteroides gin ... | 1989 | 2531793 |
| [polyclonal b cell activation induced by actinomyces viscosus t14v. responsiveness of human peripheral blood b cells]. | we have previously reported that the sonicate extrast from actinomyces viscosus t 14 v (av. sup) causes polyclonal b cell activation (pba) in murine splenic b cells without any t cell assistance. however, there are some reports describing t cell dependency of pba induced by a. viscosus in human peripheral blood b cells, and it is still controversial whether or not a. viscosus has the capability of exhibiting pba without the help of t cells. in this report, we examined pba in human peripheral blo ... | 1989 | 2535156 |
| relative adherence of bacteroides species and strains to actinomyces viscosus on saliva-coated hydroxyapatite. | the study was designed to compare the adherence of several bacteroides species to a. viscosus. using 3h, we labeled 24 laboratory strains, including 13 bacteroides species and 11 fresh clinical isolates of three bacteroides species. their adherence to a. viscosus bound to a saliva-coated mineral surface was quantified by liquid scintillation. adherence relative to a standard strain, b. gingivalis 2561, was compared. among the lab bacteroides, those of b. gingivalis (eight strains) were the great ... | 1989 | 2550532 |
| actinomyces viscosus fibril antigens detected by immunogold electron microscopy. | strains representing taxonomic clusters of actinomyces viscosus and actinomyces naeslundii were studied by indirect immunogold electron microscopy with either monospecific anti-type 1 and anti-type 2 rabbit antibodies or species-specific monoclonal antibodies. the monoclonal and anti-type 2 antibodies localized on long fibrils, whereas the anti-type 1 antibodies mostly localized close to the cell body or on shorter appendages. | 1989 | 2564376 |
| analysis of the serum antibody responses to type 1 and type 2 fimbriae in mice immunized with actinomyces viscosus t14v. | the antibody responses of inbred mice immunized with actinomyces viscosus t14v cells were analyzed using enzyme-linked immunoabsorbent assays (elisas) for measuring serum antibodies reactive with a. viscosus t14v cells and type 1 and type 2 fimbriae. in a/j mice immunized intraperitoneally on days 0 and 14, the serum antibody responses approached peak levels during d 19-35, and a dose of 10(8) cells/injection elicited optimal responses. analysis of the responses of three genetically diverse stra ... | 1989 | 2566672 |
| pellicle receptors for actinomyces viscosus type 1 fimbriae in vitro. | actinomyces viscosus t14v-j1 and its fimbria-deficient mutant strain possessing type 1 fimbriae strongly aggregated with latex beads treated with acidic proline-rich protein 1, basic proline-rich proteins, and proline-rich glycoprotein and its deglycosylated derivative. these type 1+ strains did not aggregate with latex beads treated with other proteins, such as salivary amylase, salivary histidine-rich polypeptides, laminin, type 1 collagen, fibronectin, or c1q. the type 1+ strains also adsorbe ... | 1989 | 2570751 |