Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effect of probiotic lactobacillus strains on acute diarrhea in a cohort of nonhospitalized children attending day-care centers. | certain strains of lactobacilli have been shown to promote recovery from rotavirus enteritis in hospitalized children. few studies have examined the effect of probiotics in nonhospitalized children with mild diarrhea. | 2002 | 12150179 |
| stimulation of butyrate production by gluconic acid in batch culture of pig cecal digesta and identification of butyrate-producing bacteria. | gluconic acid reaches the large intestine to stimulate lactic acid bacteria. however, the fermentation pattern of gluconic acid has yet to be elucidated. accordingly, we examined the fermentation properties induced by gluconic acid in the pig cecal digesta in vitro. we also tested sorbitol and glucose, substrates for which the fermentation rate and patterns are known. the gluconic acid-utilizing bacteria were further isolated from pig cecal digesta and identified to examine the effect of gluconi ... | 2002 | 12163667 |
| effect of probiotics on constipation, fecal azoreductase activity and fecal mucin content in the elderly. | constipation is a common problem in elderly subjects, probiotics have been suggested to improve intestinal motility and reduce fecal enzyme activity. | 2002 | 12169860 |
| supplementation of infant formula with the probiotic lactobacillus reuteri and zinc: impact on enteric infection and nutrition in infant rhesus monkeys. | gut colonization by may have beneficial effects on infant health or capacity to resist infectious disease. zinc supplementation has also been proposed to increase infants' resistance to disease; however, many studies have yielded conflicting results. | 2002 | 12187291 |
| molecular characterization of a novel glucosyltransferase from lactobacillus reuteri strain 121 synthesizing a unique, highly branched glucan with alpha-(1-->4) and alpha-(1-->6) glucosidic bonds. | lactobacillus reuteri strain 121 produces a unique, highly branched, soluble glucan in which the majority of the linkages are of the alpha-(1-->4) glucosidic type. the glucan also contains alpha-(1-->6)-linked glucosyl units and 4,6-disubstituted alpha-glucosyl units at the branching points. using degenerate primers, based on the amino acid sequences of conserved regions from known glucosyltransferase (gtf) genes from lactic acid bacteria, the l. reuteri strain 121 glucosyltransferase gene (gtfa ... | 2002 | 12200277 |
| characterization of a novel fructosyltransferase from lactobacillus reuteri that synthesizes high-molecular-weight inulin and inulin oligosaccharides. | fructosyltransferase (ftf) enzymes produce fructose polymers (fructans) from sucrose. here, we report the isolation and characterization of an ftf-encoding gene from lactobacillus reuteri strain 121. a c-terminally truncated version of the ftf gene was successfully expressed in escherichia coli. when incubated with sucrose, the purified recombinant ftf enzyme produced large amounts of fructo-oligosaccharides (fos) with beta-(2-->1)-linked fructosyl units, plus a high-molecular-weight fructan pol ... | 2002 | 12200292 |
| lactobacillus reuteri dsm 20016: purification and characterization of a cystathionine gamma-lyase and use as adjunct starter in cheesemaking. | a homo-tetrameric approximately 160-kda cystathionine gamma-lyase was purified to homogeneity from lactobacillus reuteri dsm 20016 by four chromatographic steps. the activity was pyridoxal-5'-phosphate dependent and the enzyme catalyzed the alpha,gamma-elimination reaction of l-cystathionine, producing l-cysteine, ammonia and alpha-ketobutyrate. the enzyme was active towards a range of amino acids and amino acid derivatives, including methionine. the ph and temperature optima were found to be 8. ... | 2002 | 12222803 |
| production and stability of 3-hydroxypropionaldehyde in lactobacillus reuteri. | 3-hydroxypropionaldehyde (3-hpa) is considered as a potent antimicrobial substance. exploration of its application as a food preservative or as a therapeutic auxiliary agent has been documented in the literature. in the present work, factors that may impact on 3-hpa accumulation by lactobacillus reuteri and on the stability of 3-hpa were investigated. three media - h(2)o, milk and mrs broth - were chosen as test systems. data indicated that 3-hpa accumulation in resting cells of l. reuteri in a ... | 2002 | 12382044 |
| preservation and fermentation: past, present and future. | preservation of food and beverages resulting from fermentation has been an effective form of extending the shelf-life of foods for millennia. traditionally, foods were preserved through naturally occurring fermentations, however, modern large scale production generally now exploits the use of defined strain starter systems to ensure consistency and quality in the final product. this review will mainly focus on the use of lactic acid bacteria (lab) for food improvement, given their extensive appl ... | 2002 | 12382680 |
| contribution of reutericyclin production to the stable persistence of lactobacillus reuteri in an industrial sourdough fermentation. | reutericyclin is a small molecular weight antibiotic produced by the sourdough isolate lactobacillus reuteri lth2584. this strain was isolated from an industrial sourdough, ser, in 1988. to determine whether reutericyclin formation contributes to the stable persistence of l. reuteri in sourdough, evaluations were made on whether reutericyclin-producing strains were among l. reuteri isolates from the ser sourdough obtained in 1994 and 1998. these strains were characterised on species and strain l ... | 2003 | 12430769 |
| purification of spirosin from lactobacillus reuteri dsm 20016 and partial characterization based on its amino acid sequences. | 1998 | 12501286 | |
| a natural compound (reuterin) produced by lactobacillus reuteri for biological-tissue fixation. | the study was undertaken to examine the degree of tissue fixation by reuterin, a natural compound produced by lactobacillus reuteri, at distinct fixation conditions (ph, temperature, and fixative concentration). additionally, the rate of tissue fixation by reuterin was investigated using glutaraldehyde as a control. it was found by the fourier transformed infrared spectroscopy and nuclear magnetic resonance spectroscopy that both mono- and di-aldehyde reuterin oligomers may be present in the aci ... | 2003 | 12527275 |
| kinetic properties of an inulosucrase from lactobacillus reuteri 121. | inulosucrases catalyze transfer of a fructose moiety from sucrose to a water molecule (hydrolysis) or to an acceptor molecule (transferase), yielding inulin. bacterial inulin production is rare and a biochemical analysis of inulosucrase enzymes has not been reported. here we report biochemical characteristics of a purified recombinant inulosucrase enzyme from lactobacillus reuteri. it displayed michaelis-menten type of kinetics with substrate inhibition for the hydrolysis reaction. kinetics of t ... | 2003 | 12527388 |
| role of microecology in chronic inflammatory bowel diseases. | inflammatory bowel diseases (ibd) are chronic conditions of unknown etiology. current therapy mitigates the severity of acute bouts of mucosal inflammation but an eradication therapy is lacking. growing incidence of ibd is associated with social development. epidemiology suggests a relationship between the establishment of the individual gut flora and the risk of developing ibd. patients show an impaired tolerance towards commensal bacteria of the resident flora. unrestrained activation of the i ... | 2002 | 12556945 |
| evaluation of the effect of malt, wheat and barley extracts on the viability of potentially probiotic lactic acid bacteria under acidic conditions. | in this work, the effect of cereal extracts, used as delivery vehicles for potentially probiotic lactic acid bacteria (lab), on the acid tolerance of the cells was evaluated under conditions that simulate the gastric tract. more specifically, the effect of malt, barley and wheat extracts on the viability of lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri during exposure for 4 h in a phosphate buffer acidified at ph 2.5 was investigated. in the absence of cereal extra ... | 2003 | 12568753 |
| in situ production of exopolysaccharides during sourdough fermentation by cereal and intestinal isolates of lactic acid bacteria. | eps formed by lactobacilli in situ during sourdough fermentation may replace hydrocolloids currently used as texturizing, antistaling, or prebiotic additives in bread production. in this study, a screening of >100 strains of cereal-associated and intestinal lactic acid bacteria was performed for the production of exopolysaccharides (eps) from sucrose. fifteen strains produced fructan, and four strains produced glucan. it was remarkable that formation of glucan and fructan was most frequently fou ... | 2003 | 12571016 |
| effect of reuterin produced by lactobacillus reuteri on the surface of sausages to inhibit the growth of listeria monocytogenes and salmonella spp. | reuterin is a bacteriocin produced by some strains of lactobacillus reuteri. the strain used in this study was isolated from raw milk from a dairy farm nearby ankara. beef sausage is a long years produced bratwurst style meat product in turkey, as well as in some other countries in the mediterranean region. sausages are produced by raw meat; sometimes lactic starter cultures are added or spontaneous fermentation is employed. the production and storage conditions of the product promotes the growt ... | 2002 | 12577589 |
| effect of probiotic lactobacillus strains in children with atopic dermatitis. | recent studies suggest that oral bacteriotherapy with probiotics might be useful in the management of atopic dermatitis (ad). | 2003 | 12589361 |
| lactobacillus growth and membrane composition in the presence of linoleic or conjugated linoleic acid. | five lactobacillus strains of intestinal and food origins were grown in mrs broth or milk containing various concentrations of linoleic acid or conjugated linoleic acid (cla). the fatty acids had bacteriostatic, bacteriocidal, or no effect depending on bacterial strain, fatty acid concentration, fatty acid type, and growth medium. both fatty acids displayed dose-dependent inhibition. all strains were inhibited to a greater extent by the fatty acids in broth than in milk. the cla isomer mixture w ... | 2003 | 12674348 |
| identification of lactobacillus reuteri genes specifically induced in the mouse gastrointestinal tract. | lactobacilli are common inhabitants of the gastrointestinal tracts of mammals and have received considerable attention due to their putative health-promoting properties. little is known about the traits that enhance the ability of these bacteria to inhabit the gastrointestinal tract. in this paper we describe the development and application of a strategy based on in vivo expression technology (ivet) that enables detection of lactobacillus reuteri genes specifically induced in the murine gut. a p ... | 2003 | 12676681 |
| purification and structural characterization of 3-hydroxypropionaldehyde and its derivatives. | the compound 3-hydroxypropionaldehyde (3-hpa), together with hpa hydrate and hpa dimer, in aqueous solution forms a system with interesting chemical properties. therefore, 3-hpa has attracted attention by the chemical industry for use as a precursor in the production of plastics, acrylic acid, and 1,3-propanediol and by the food industry, in using 3-hpa-producing lactobacillus reuteri as a probiotic. to produce 3-hpa in high yield from glycerol, l. reuteri was used as a biotransformation system. ... | 2003 | 12744656 |
| bioconversion of linoleic acid into conjugated linoleic acid by immobilized lactobacillus reuteri. | lactobacillus reuteri was immobilized on silica gel to evaluate the bioconversion of linoleic acid (la) into conjugated linoleic acid (cla), consisting of cis-9,trans-11 and trans-10,cis-12 isomers. the amount of cell to carrier, the reaction time, and the substrate concentration, ph, and temperature for cla production were optimized at 10 mg of cells/(g of carrier), 1 h, 500 mg/l la, 10.5, and 55 degrees c, respectively. in the presence of 1.0 mm cu(2+), cla production increased by 110%. under ... | 2003 | 12790685 |
| identification of heterofermentative lactobacilli isolated from pig faeces by numerical analysis of total soluble cell protein patterns and rapd-pcr. | the study deals with a number of heterofermentative lactobacillus strains isolated from pig faeces and their identification. | 2003 | 12803548 |
| bile salts and cholesterol induce changes in the lipid cell membrane of lactobacillus reuteri. | the objective of this study was to evaluate the effect of bile salts and cholesterol in the lipid profile of lactobacillus reuteri crl 1098 and to determine the relationship existing between these changes: the in vitro removal of cholesterol and the tolerance of the cells to acid and cold stress. | 2003 | 12807457 |
| bioconversion of linoleic acid into conjugated linoleic acid during fermentation and by washed cells of lactobacillus reuteri. | conjugated linoleic acid (cla) was produced at 300 mg l(-1) after 24 h culture of lactobacillus reuteri in de man-rogosa-sharpe medium containing 0.9 g linoleic acid (la) l(-1) and 1.67% (v/v) tween 80. cla was mainly located in the extracellular space of the cells. washed cells previously grown on la were less active than unadapted washed cells in converting la into cla. most of the cla transformed by washed l. reuteri cells was located in cells or associated with cells. cla production by washe ... | 2003 | 12889826 |
| reuterin production by lactobacilli isolated from pig faeces and evaluation of probiotic traits. | to determine the production of reuterin by lactobacilli isolates from pig faeces and to evaluate their potential as probiotic bacteria. | 2003 | 12904230 |
| growth and metabolism of selected strains of probiotic bacteria in milk. | growth and metabolism of five probiotic strains with well-documented health effects were studied in ultra-high temperature (uht) treated milk, supplemented with 0.5% (w/v) tryptone or 0.75% (w/v) fructose. the probiotic strains were lactobacillus acidophilus la5, lb. acidophilus 1748, lactobacillus rhamnosus gg, lactobacillus reuteri sd 2112 and bifidobacterium animalis bb12. fermentation was followed for 72 h at 37 degrees c and the samples were analysed for ph, log cfu ml(-1), volatile compoun ... | 2003 | 12927703 |
| lactobacillus reuteri crl1098 produces cobalamin. | we found that lactobacillus reuteri crl1098, a lactic acid bacterium isolated from sourdough, is able to produce cobalamin. the sugar-glycerol cofermentation in vitamin b(12)-free medium showed that this strain was able to reduce glycerol through a well-known cobalamin-dependent reaction with the formation of 1,3-propanediol as a final product. the cell extract of l. reuteri corrected the coenzyme b12 requirement of lactobacillus delbrueckii subsp. lactis atcc 7830 and allowed the growth of salm ... | 2003 | 12949118 |
| synthesis of alpha-galactooligosaccharides with alpha-galactosidase from lactobacillus reuteri of canine origin. | crude cell-free extracts from lactobacillus reuteri grown on cellobiose, maltose, lactose and raffinose were assayed for glycosidic activities. when raffinose was used as the carbon source, alpha-galactosidase was produced, showing the highest yield at the beginning of the stationary growth phase. a 64 kda enzyme was purified by ultra- and gel filtration, and characterized for its hydrolytic and synthetic activity. highest hydrolytic activity was found at ph 5.0 at 50 degrees c ( k(m) 0.55 mm, v ... | 2003 | 12955354 |
| natural antimicrobial agent (reuterin) produced by lactobacillus reuteri for sanitization of biological tissues inoculated with pseudomonas aeruginosa. | the study was done to evaluate the efficacy of using reuterin produced by lactobacillus reuteri to sanitize biological tissues. the microorganism tested in the study was pseudomonas aeruginosa, a common cause of nosocomial biomaterial-related infections. the inhibitory effect of reuterin on p. aeruginosa for an inoculated tissue was investigated at different conditions of concentration, temperature, and ph. additionally, the cellular compatibility of the reuterin-sanitized tissue was evaluated. ... | 2003 | 12966580 |
| in vitro evaluation of the fermentation properties of galactooligosaccharides synthesised by alpha-galactosidase from lactobacillus reuteri. | stirred, ph-controlled anaerobic batch cultures were used to evaluate the in vitro utilisation by canine gut microflora of novel alpha-galactooligosaccharides synthesised with an enzyme extract from a canine lactobacillus reuteri strain. fructooligosaccharides (fos), melibiose and raffinose were used as reference carbohydrates for the prebiotic properties of the synthesised oligosaccharide (galactosyl melibiose mixture-gmm). addition of lactobacillus acidophilus was used as control for the evalu ... | 2004 | 13680200 |
| peptide surface display and secretion using two lpxtg-containing surface proteins from lactobacillus fermentum br11. | a locus encoding two repetitive proteins that have lpxtg cell wall anchoring signals from lactobacillus fermentum br11 has been identified by using an antiserum raised against whole l. fermentum br11 cells. the first protein, rlp, is similar to the rib surface protein from streptococcus agalactiae, while the other protein, mlp, is similar to the mucus binding protein mub from lactobacillus reuteri. it was shown that multiple copies of mlp exist in the genome of l. fermentum br11. regions of rlp, ... | 2003 | 14532035 |
| detection and identification of lactobacillus species in crops of broilers of different ages by using pcr-denaturing gradient gel electrophoresis and amplified ribosomal dna restriction analysis. | the microflora of the crop was investigated throughout the broiler production period (0 to 42 days) using pcr combined with denaturing gradient gel electrophoresis (pcr-dgge) and selective bacteriological culture of lactobacilli followed by amplified ribosomal dna restriction analysis (ardra). the birds were raised under conditions similar to those used in commercial broiler production. lactobacilli predominated and attained populations of 10(8) to 10(9) cfu per gram of crop contents. many of th ... | 2003 | 14602636 |
| bactericidal effects of lactobacillus reuteri and allyl isothiocyanate on escherichia coli o157:h7 in refrigerated ground beef. | two naturally occurring antimicrobial agents were tested in packages of refrigerated ground beef for their ability to reduce the viability of escherichia coli o157:h7 during storage. allyl isothiocyanate (aitc) and lactobacillus reuteri were tested separately and together for their action against a cocktail of five strains of e. coli o157:h7 in ground beef held at 4 degrees c for 25 days. ground beef prepared from whole, raw inside round beef roasts was inoculated with low (3 log cfu/g) or high ... | 2003 | 14627280 |
| heat shock treatment increases the frequency of loss of an erythromycin resistance-encoding transposable element from the chromosome of lactobacillus crispatus chcc3692. | a 3,165-bp chromosomally integrated transposon, designatedtn3692, of the gram-positive strain lactobacillus crispatus chcc3692 contains an erm(b) gene conferring resistance to erythromycin at concentrations of up to 250 micrograms/ml. loss of this resistance can occur spontaneously, but the rate is substantially increased by heat shock treatment. heat shock treatment at 60 degrees c resulted in an almost 40-fold increase in the frequency of erythromycin-sensitive cells (erythromycin mic, 0.047 m ... | 2003 | 14660363 |
| phage display reveals 52 novel extracellular and transmembrane proteins from lactobacillus reuteri dsm 20016(t). | extracellular and transmembrane proteins are important for the binding of bacteria to intestinal surfaces and for their interaction with the host. the aim of this study was to identify genes encoding extracellular and transmembrane proteins from the probiotic bacterium lactobacillus reuteri by construction and screening of a phage display library. this library was constructed by insertion of randomly fragmented dna from l. reuteri into the phagemid vector pg3dss, which was previously developed f ... | 2003 | 14663082 |
| 3-hydroxypropionaldehyde: applications and perspectives of biotechnological production. | 3-hydroxypropionaldehyde (3-hpa) forms, together with hpa-hydrate and hpa-dimer, a dynamic, multi-component system (hpa system) used in food preservation, as a precursor for many modern chemicals such as acrolein, acrylic acid, and 1,3-propanediol (1,3-pdo), and for polymer production. 3-hpa can be obtained both through traditional chemistry and bacterial fermentation. to date, 3-hpa has been produced from petrochemical resources as an intermediate in 1,3-pdo production. in vivo, glycerol is con ... | 2004 | 14669058 |
| streptococcus alactolyticus is the dominating culturable lactic acid bacterium species in canine jejunum and feces of four fistulated dogs. | canine intestinal lactic acid bacterium (lab) population in four fistulated dogs was cultured and enumerated using mrs agar. lab levels ranging from 1.4x10(6) to 1.5x10(7) cfu ml(-1) were obtained in jejunal chyme. in the fecal samples 7.0x10(7) and 2.0x10(8) cfu g(-1) were detected. thirty randomly selected isolates growing in the highest sample dilutions were identified to species level using numerical analysis of 16s and 23s rdna restriction fragment length polymorphism patterns (ribotyping) ... | 2004 | 14734163 |
| reutericyclin: biological activity, mode of action, and potential applications. | reutericyclin is an inhibitory compound produced by sourdough isolates of lactobacillus reuteri that is structurally but not functionally related to naturally occurring tetramic acids. it is bacteriostatic or bactericidal to gram-positive bacteria based on its activity as a proton-ionophore, and a broad range of food-related spoilage organisms and pathogens is inhibited by reutericyclin. gram-negative bacteria are resistant to reutericyclin because of the barrier properties of their outer membra ... | 2004 | 14735324 |
| probiotics in gastroenterology: indications and future perspectives. | nowadays. there is a growing interest in probiotics as a safe way of changing the intestinal bacterial flora. probiotics may have potential in several gastroenterological conditions, especially when the intestinal flora has been disturbed. most scientific evidence is available for diarrhoea patients treated with lactobacillus gg, lactobacillus reuteri or saccharomyces boulardii. meta-analyses have shown an overall reduction in the risk of antibiotic-associated diarrhoea during treatment with pro ... | 2003 | 14743878 |
| binding of bifidobacterium bifidum and lactobacillus reuteri to the carbohydrate moieties of intestinal glycolipids recognized by peanut agglutinin. | we examined binding of bifidobacterium bifidum and lactobacillus reuteri to the carbohydrate moieties of glycolipids extracted from human enterocyte-like caco-2 cells in this study. in binding assays to reference glycolipids of different carbohydrate compositions, b. bifidum eb102 bound strongly to gangliotetraosylceramide (asialo-gm1) and less strongly to gangliotriaosylceramide (asialo-gm2), lactosylceramide and sulfatide. the binding profile of b. bifidum eb102 was almost identical to that of ... | 2004 | 14751691 |
| colonization and immunomodulation by lactobacillus reuteri atcc 55730 in the human gastrointestinal tract. | lactobacillus reuteri atcc 55730 is a probiotic (health-promoting) bacterium widely used as a dietary supplement. this study was designed to examine local colonization of the human gastrointestinal mucosa after dietary supplementation with l. reuteri atcc 55730 and to determine subsequent immune responses at the colonized sites. in this open clinical investigation, 10 healthy volunteers and 9 volunteers with ileostomy underwent gastroscopy or ileoscopy and biopsy samples were taken from the stom ... | 2004 | 14766603 |
| growth and metabolism of selected strains of probiotic bacteria, in maize porridge with added malted barley. | a fermented probiotic maize porridge with high energy density and low viscosity was prepared, using maize flour and barley malt. the porridge was fermented with four probiotic strains (grown separately): lactobacillus reuteri, lb. acidophilus (la5 and 1748) and lb. rhamnosus gg. these strains were inoculated at two levels; to obtain approx. 7 or 6 log cfu g(-1) in the porridge at 0 h. the porridge was fermented for 24 h at 37 degrees c, and analysed for viable cell count, ph, organic acids, vola ... | 2004 | 14984778 |
| site-directed mutagenesis study of the three catalytic residues of the fructosyltransferases of lactobacillus reuteri 121. | bacterial fructosyltransferases (ftfs) are retaining-type glycosidases that belong to family 68 of glycoside hydrolases. recently, the high-resolution 3d structure of the bacillus subtilis levansucrase has been solved [meng, g. and futterer, k., nat. struct. biol. 10 (2003) 935-941]. based on this structure, the catalytic nucleophile, general acid/base catalyst, and transition state stabilizer were identified. however, a detailed characterization of site-directed mutants of the catalytic nucleop ... | 2004 | 14988011 |
| biochemical and molecular characterization of a levansucrase from lactobacillus reuteri. | lactobacillus reuteri strain 121 employs a fructosyltransferase (ftf) to synthesize a fructose polymer [a fructan of the levan type, with beta(2-->6) linkages] from sucrose or raffinose. purification of this ftf (a levansucrase), and identification of peptide amino acid sequences, allowed isolation of the first lactobacillus levansucrase gene (lev), encoding a protein (lev) consisting of 804 amino acids. lev showed highest similarity with an inulosucrase of l. reuteri 121 [inu; producing an inul ... | 2004 | 14993311 |
| light sensitivity of bifidobacterium longum in bioreactor cultivations. | bifidobacteria are gaining commercial significance due to their probiotic properties. however, little is still known about the production of these bacteria and their behavior in bioreactors. two bifidobacterium longum strains were sensitive to light when grown in a transparent (glass) bioreactor under microaerophilic growth conditions (i.e. no gases added and slow mixing). the sensitivity was less clear the more anaerobic the initial conditions were. in a darkened bioreactor in microaerophilic c ... | 2004 | 15127799 |
| animal model for in vivo evaluation of cholesterol reduction by lactic acid bacteria. | for many years, it has been recognized that elevated serum cholesterol is a risk factor associated with atherosclerosis and coronary heart disease, the latter being a major cause of death in western countries. numerous drugs that lower cholesterol have been used to treat hypocholesterolemic individuals. however, the undesirable side effects of these compounds have caused concerns about their therapeutic use. ingestion of probiotic (beneficial for health) lactic acid bacteria (lab) would possibly ... | 2004 | 15156052 |
| identification and characterization of the novel lysm domain-containing surface protein sep from lactobacillus fermentum br11 and its use as a peptide fusion partner in lactobacillus and lactococcus. | examination of supernatant fractions from broth cultures of lactobacillus fermentum br11 revealed the presence of a number of proteins, including a 27-kda protein termed sep. the amino-terminal sequence of sep was determined, and the gene encoding it was cloned and sequenced. sep is a 205-amino-acid protein and contains a 30-amino-acid secretion signal and has overall homology (between 39 and 92% identity) with similarly sized proteins of lactobacillus reuteri, enterococcus faecium, streptococcu ... | 2004 | 15184172 |
| a natural compound (reuterin) produced by lactobacillus reuteri for hemoglobin polymerization as a blood substitute. | stroma-free hemoglobin (hb) has been modified by pyridoxylation and followed by polymerization with glutaraldehyde as a blood substitute. nevertheless, the reaction rate of pyridoxylated hb (plp-hb) with glutaraldehyde is too fast to control its molecular weight distribution. additionally, it was reported that glutaraldehyde is cytotoxic even at low doses. to overcome these problems, another aldehyde, beta-hydroxypropionaldehyde (beta-hpa), was used in the study to polymerize hemoglobin (plp-hb) ... | 2004 | 15211486 |
| specific response of a novel and abundant lactobacillus amylovorus-like phylotype to dietary prebiotics in the guts of weaning piglets. | using 16s rrna gene-based approaches, we analyzed the responses of ileal and colonic bacterial communities of weaning piglets to dietary addition of four fermentable carbohydrates (inulin, lactulose, wheat starch, and sugar beet pulp). an enriched diet and a control diet lacking these fermentable carbohydrates were fed to piglets for 4 days (n = 48), and 10 days (n = 48), and the lumen-associated microbiota were compared using denaturing gradient gel electrophoresis (dgge) analysis of amplified ... | 2004 | 15240251 |
| biochemical and molecular characterization of lactobacillus reuteri 121 reuteransucrase. | lactobacillus reuteri strain 121 uses sucrose for synthesis of a unique, soluble glucan ('reuteran') with mainly alpha-(1-->4) glucosidic linkages. the gene (gtfa) encoding this glucansucrase enzyme had previously been characterized. here, a detailed biochemical and molecular analysis of the gtfa enzyme is presented. this is believed to be the first report describing reuteransucrase enzyme kinetics and the oligosaccharides synthesized with various acceptors. alignments of the gtfa sequence with ... | 2004 | 15256553 |
| lactobacillus reuteri in bovine milk fermented decreases the oral carriage of mutans streptococci. | the effect of lactobacillus reuteri against one of the major cariogenic organism, streptococcus mutans, was studied. yogurt products containing l.reuteri showed a significant growth inhibitory effect against s. mutans, whilst yoghurts with lactobaccilli other than l. reuteri did not show such inhibition. further, double-blind, placebo-controlled trial demonstrated that consuming yogurt with l. reuteri significantly reduced the oral carriage of mutans streptococci, compared with the placebo yogur ... | 2004 | 15282133 |
| expression of a heterologous manganese superoxide dismutase gene in intestinal lactobacilli provides protection against hydrogen peroxide toxicity. | in living organisms, exposure to oxygen provokes oxidative stress. a widespread mechanism for protection against oxidative stress is provided by the antioxidant enzymes: superoxide dismutases (sods) and hydroperoxidases. generally, these enzymes are not present in lactobacillus spp. in this study, we examined the potential advantages of providing a heterologous sod to some of the intestinal lactobacilli. thus, the gene encoding the manganese-containing sod (soda) was cloned from streptococcus th ... | 2004 | 15294805 |
| live lactobacillus reuteri is essential for the inhibitory effect on tumor necrosis factor alpha-induced interleukin-8 expression. | the mechanism of the apparent anti-inflammatory action of probiotic organisms is unclear. lactobacillus reuteri is effective in inhibiting colitis in interleukin-10 (il-10)-deficient mice. nerve growth factor (ngf), in addition to its activity on neuronal cell growth, has significant anti-inflammatory effects in several experimental systems in vitro and in vivo, including a model of colitis. our experiments were designed to explore the mechanism of effect of l. reuteri in the human epithelial ce ... | 2004 | 15322027 |
| influence of adjunct cultures on volatile free fatty acids in reduced-fat edam cheeses. | the effects of the adjunct cultures lactococcus lactis ssp. diacetylactis, brevibacterium linens bl2, lactobacillus helveticus lh212, and lactobacillus reuteri atcc 23272 on volatile free fatty acid production in reduced-fat edam cheese were studied. lipase activity evaluation using p-nitrophenyl fatty acid ester substrates indicated that l. lactis ssp. diacetylactis showed the highest activity among the 4 adjunct cultures. full-fat and 33% reduced-fat control cheeses (no adjunct) were made alon ... | 2004 | 15377601 |
| rapid identification of probiotic lactobacillus species by multiplex pcr using species-specific primers based on the region extending from 16s rrna through 23s rrna. | this study aimed to develop a novel multiplex polymerase chain reaction (pcr) primer set for the identification of seven probiotic lactobacillus species such as lactobacillus acidophilus, lactobacillus delbrueckii, lactobacillus casei, lactobacillus gasseri, lactobacillus plantarum, lactobacillus reuteri and lactobacillus rhamnosus. the primer set, comprising of seven specific and two conserved primers, was derived from the integrated sequences of 16s and 23s rrna genes and their rrna intergenic ... | 2004 | 15476976 |
| glucan synthesis in the genus lactobacillus: isolation and characterization of glucansucrase genes, enzymes and glucan products from six different strains. | members of the genera streptococcus and leuconostoc synthesize various alpha-glucans (dextran, alternan and mutan). in lactobacillus, until now, the only glucosyltransferase (gtf) enzyme that has been characterized is gtfa of lactobacillus reuteri 121, the first gtf enzyme synthesizing a glucan (reuteran) that contains mainly alpha-(1-->4) linkages together with alpha-(1-->6) and alpha-(1-->4,6) linkages. recently, partial sequences of glucansucrase genes were detected in other members of the ge ... | 2004 | 15528655 |
| artificial cell microcapsule for oral delivery of live bacterial cells for therapy: design, preparation, and in-vitro characterization. | bacterial cells can be engineered to synthesize a wide array of disease modifying substrates such as cytokines, vaccines and antibodies; however, their use as an orally delivered therapeutic is limited by poor gastrointestinal (gi) survival and instigation of immunogenic response. artificial cell microcapsules have been well studied as a means to overcome such problems, however, presently obtainable microcapsules have limitations. this study summarizes a novel microcapsule design specifying its ... | 2004 | 15576011 |
| effect of a probiotic infant formula on infections in child care centers: comparison of two probiotic agents. | to investigate the effect of 2 different species of probiotics in preventing infections in infants attending child care centers. | 2005 | 15629974 |
| lactobacillus reuteri atcc 53608 mdh gene cloning and recombinant mannitol dehydrogenase characterization. | a gene encoding mannitol-2-dehydrogenase (e.c. 1.1.1.138) (mdh) was cloned from lactobacillus reuteri and expressed in escherichia coli. the 1,008-bp gene encodes a protein consisting of 336 amino acids, with a predicted molecular mass of 35,920 da. the deduced amino acid sequence of l. reuteri mdh (lrmdh) is 77% and 76% similar to the mdhs from leuconostoc mesenteroides and leuconostoc pseudomesenteroides, respectively. the purified recombinant enzyme appears as a single band of 40 kda in sodiu ... | 2005 | 15630578 |
| effects of the administration of lactobacilli on body growth and on the metabolic profile in growing maltese goat kids. | the aim of this study was to evaluate the effect of some lactobacilli on body growth and on the metabolic-nutritional status in growing goat kids. twenty growing maltese goat kids (10 control and 10 bios) were studied. the animals of the bios group received a concentrate including 1 g x kg(-1) of seb bovino (spray-dried), akron s.r.l., italy, with non bacterial components: gum arabic, soybean meal, silicate alum of magnesium, and with bacterial components: 10(11) cfu kg(-1) each of lactobacillus ... | 2004 | 15636163 |
| lactobacillus gastricus sp. nov., lactobacillus antri sp. nov., lactobacillus kalixensis sp. nov. and lactobacillus ultunensis sp. nov., isolated from human stomach mucosa. | in an attempt to study the composition of the lactobacillus flora from mucosa of human stomach, 16s rrna gene sequences of 129 isolates were analysed. for 15 of these, the results differed significantly from known sequences, and additional tests were performed to determine whether these isolates represented as yet unrecognized species. phenotypic and genetic characteristics revealed that these isolates represented four novel lactobacillus species. two belong to the lactobacillus reuteri and the ... | 2005 | 15653856 |
| probiotic lactobacillus spp. diminish helicobacter hepaticus-induced inflammatory bowel disease in interleukin-10-deficient mice. | clinical and experimental evidence has demonstrated the potential role of probiotics in the prevention or treatment of inflammatory bowel disease. probiotic clones with direct immunomodulatory activity may have anti-inflammatory effects in the intestine. we investigated the roles of tumor necrosis factor alpha (tnf-alpha)-inhibitory lactobacillus clones with a pathogen-induced murine colitis model. murine-derived probiotic lactobacilli were selected in vitro for their ability to inhibit tnf-alph ... | 2005 | 15664933 |
| production of 3-hydroxypropionaldehyde using a two-step process with lactobacillus reuteri. | 3-hydroxypropionaldehyde (3-hpa) produced by lactobacillus reuteri is a broad-spectrum antimicrobial substance of glycerol conversion. the aim of the present work was to optimize 3-hpa production by lb. reuteri atcc 53608 using a two-step process. the first step was the production of lb. reuteri cells in optimal conditions. cells were then harvested by centrifugation and suspended in glycerol solution, which the resting cells bioconverted to 3-hpa. the effect of biomass concentration, temperatur ... | 2005 | 15682289 |
| a high-molecular-mass surface protein (lsp) and methionine sulfoxide reductase b (msrb) contribute to the ecological performance of lactobacillus reuteri in the murine gut. | members of the genus lactobacillus are common inhabitants of the gut, yet little is known about the traits that contribute to their ecological performance in gastrointestinal ecosystems. lactobacillus reuteri 100-23 persists in the gut of the reconstituted lactobacillus-free mouse after a single oral inoculation. recently, three genes of this strain that were specifically induced (in vivo induced) in the murine gut were identified (38). we report here the detection of a gene of l. reuteri 100-23 ... | 2005 | 15691956 |
| mutational analysis of the role of calcium ions in the lactobacillus reuteri strain 121 fructosyltransferase (levansucrase and inulosucrase) enzymes. | bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (gh68) are not known to require a metal cofactor. here, we show that ca2+ ions play an important structural role in the lactobacillus reuteri 121 levansucrase (lev) and inulosucrase (inu) enzymes. analysis of the bacillus subtilis lev 3d structure [meng, g. and futterer, k. (2003) nat. struct. biol. 10, 935-941] has provided evidence for the presence of a bound metal ion, most likely ca2+. characterization of site- ... | 2005 | 15710400 |
| effects of probiotic bacteria on humoral immunity to candida albicans in immunodeficient bg/bg-nu/nu and bg/bg-nu/+ mice. | germfree beige-nude ( bg/bg-nu/nu) and beige-heterozygous ( bg/bg-nu/+) mice were colonized with a pure culture of candida albicans or with a probiotic bacterium (lactobacillus acidophilus, lactobacillus reuteri, lactobacillus casei, or bifidobacterium infantis). probiotic-colonized mice were subsequently challenged orally with c. albicans. the effect of prior colonization with probiotic bacteria on the antibody responses of the immunodeficient mice to alimentary tract colonization with c. albic ... | 2000 | 15813696 |
| influence of dietary zinc oxide and copper sulfate on the gastrointestinal ecosystem in newly weaned piglets. | dietary doses of 2,500 ppm zno-zn reduced bacterial activity (atp accumulation) in digesta from the gastrointestinal tracts of newly weaned piglets compared to that in animals receiving 100 ppm zno-zn. the amounts of lactic acid bacteria (mrs counts) and lactobacilli (rogosa counts) were reduced, whereas coliforms (macconkey counts) and enterococci (slanetz counts, red colonies) were more numerous in animals receiving the high zno dose. based on 16s rrna gene sequencing, the colonies on mrs were ... | 2005 | 15870311 |
| lactobacillus reuteri beta-galactosidase activity and low milk acidification ability. | beta-galactosidase activity was studied as a possible cause of the low milk acidification ability observed in lactobacillus reuteri nrrl 14171. enzymatic activity was determined in mrs broth supplemented with either glucose or lactose and milk at the middle and final stage of the exponential phase, as well as at the stationary phase. results were compared with beta-galactosidase activity in lactobacillus casei nrrl-b1922, a strain that shows the milk acidification ability. the effects of the typ ... | 2005 | 15920624 |
| selective probiotic bacteria induce il-10-producing regulatory t cells in vitro by modulating dendritic cell function through dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin. | lactobacilli are probiotic bacteria that are frequently tested in the management of allergic diseases or gastroenteritis. it is hypothesized that these probiotics have immunoregulatory properties and promote mucosal tolerance, which is in part mediated by regulatory t cells (treg cells). on the basis of pathogenic or tissue-specific priming, dendritic cells (dc) acquire different t cell-instructive signals and drive the differentiation of naive t h cells into either t h 1, t h 2, or regulatory e ... | 2005 | 15940144 |
| rational transformation of lactobacillus reuteri 121 reuteransucrase into a dextransucrase. | glucansucrase or glucosyltransferase (gtf) enzymes of lactic acid bacteria display high sequence similarity but catalyze synthesis of different alpha-glucans (e.g., dextran, mutan, alternan, and reuteran) from sucrose. the variations in glucosidic linkage specificity observed in products of different glucansucrase enzymes appear to be based on relatively small differences in amino acid sequences in their sugar-binding acceptor subsites. this notion was derived from mutagenesis of amino acids of ... | 2005 | 15966745 |
| growth of lactic acid bacteria and rhizopus oligosporus during barley tempeh fermentation. | the zygomycete rhizopus oligosporus is traditionally used to ferment soybean tempeh, but it is also possible to ferment other legumes and cereals to tempeh. the traditional soybean tempeh harbours a multitude of microorganisms with potentially beneficial or detrimental effects on quality. lactic acid bacteria (lab) have positive effects on the safety of soybean tempeh, but the effects of lab on r. oligosporus growth have not been investigated. we have developed a cereal grain tempeh by fermentin ... | 2005 | 15979185 |
| highly hydrolytic reuteransucrase from probiotic lactobacillus reuteri strain atcc 55730. | lactobacillus reuteri strain atcc 55730 (lb bio) was isolated as a pure culture from a reuteri tablet purchased from the biogaia company. this probiotic strain produces a soluble glucan (reuteran), in which the majority of the linkages are of the alpha-(1-->4) glucosidic type ( approximately 70%). this reuteran also contains alpha-(1-->6)- linked glucosyl units and 4,6-disubstituted alpha-glucosyl units at the branching points. the lb bio glucansucrase gene (gtfo) was cloned and expressed in esc ... | 2005 | 16000808 |
| the efficacy of lactobacillus reuteri atcc 55730 in the treatment of patients with irritable bowel syndrome--a double blind, placebo-controlled, randomized study. | it was suggested that the intestinal microflora may play a role in the pathogenesis of irritable bowel syndrome (ibs). probiotics may ease symptoms in ibs patients by changing gut microflora, reducing mucosal inflammation and exerting antibacterial effects. | 2005 | 16051399 |
| lactobacillus oligofermentans sp. nov., associated with spoilage of modified-atmosphere-packaged poultry products. | unidentified lactic acid bacterium (lab) isolates which had mainly been detected in spoiled, marinated, modified atmosphere packaged (map) broiler meat products during two previous studies, were identified and analyzed for their phenotypic properties and the capability to produce biogenic amines. to establish the taxonomic position of these isolates, 16s rrna gene sequence analysis, numerical analysis of ribopatterns, and dna-dna hybridization experiments were done. unexpectedly for a meat-spoil ... | 2005 | 16085830 |
| extracellular homopolysaccharides and oligosaccharides from intestinal lactobacilli. | to characterize lactobacilli isolated from the intestines of ducks or pigs with respect to the production of extracellular homopolysaccharides (hops) and oligosaccharides. | 2005 | 16108811 |
| colitic scid mice fed lactobacillus spp. show an ameliorated gut histopathology and an altered cytokine profile by local t cells. | scid mice transplanted with cd4 t blast cells develop colitis. we investigated if the disease was influenced in colitic mice treated with antibiotic and fed lactobacillus spp. | 2005 | 16116315 |
| direct cloning of a xylanase gene from the mixed genomic dna of rumen fungi and its expression in intestinal lactobacillus reuteri. | a relatively newly defined xylanase gene, xynr8, was obtained directly from a mixed dna sample prepared from unpurified rumen fungal cultures by pcr amplification. the dna sequence of xynr8 revealed that the gene was 884 bp in size and encoded amino acid sequences with a molecular weight of 27.9 kda. xynr8 belonged to glycosyl hydrolase family 11, and the catalytic site residues were also found in its amino acid sequence. the main hydrolysis products of xynr8 were xylobiose, xylotriose and xylot ... | 2005 | 16146673 |
| inducible gene expression in lactobacillus reuteri lth5531 during type ii sourdough fermentation. | lactobacillus reuteri lth5531 is a dominant member of the microbiota of type ii sourdough fermentations. to investigate the genetic background of the ecological performance of lth5531, in vivo expression technology was used to identify promoters that show elevated levels of expression during growth of this organism in a type ii sourdough fermentation. thirty-eight sourdough-induced fusions were detected, and 29 genes could be identified on the basis of the available sequence information. four ge ... | 2005 | 16204499 |
| the cell surface of lactobacillus reuteri atcc 55730 highlighted by identification of 126 extracellular proteins from the genome sequence. | bioinformatical analyses of a draft genome sequence of the commensal bacterium lactobacillus reuteri atcc 55730 revealed 126 genes encoding putative extracellular proteins. the function, localization and distribution in bacterial species were predicted. interestingly, few proteins possessed lpxtg motifs or c-terminal transmembrane anchors. instead eight proteins were putatively anchored by gw repeats and several secreted proteins were likely to be re-associated to the surface. the majority of th ... | 2005 | 16242863 |
| expression of rumen microbial fibrolytic enzyme genes in probiotic lactobacillus reuteri. | this study was aimed at evaluating the cloning and expression of three rumen microbial fibrolytic enzyme genes in a strain of lactobacillus reuteri and investigating the probiotic characteristics of these genetically modified lactobacilli. the neocallimastix patriciarum xylanase gene xyncdbfv, the fibrobacter succinogenes beta-glucanase (1,3-1,4-beta-d-glucan 4-glucanohydrolase [ec 3.2.1.73]) gene, and the piromyces rhizinflata cellulase gene egla were cloned in a strain of l. reuteri isolated f ... | 2005 | 16269708 |
| increasing work-place healthiness with the probiotic lactobacillus reuteri: a randomised, double-blind placebo-controlled study. | short term illnesses, usually caused by respiratory or gastrointestinal diseases are disruptive to productivity and there is relatively little focus on preventative measures. this study examined the effect of the probiotic lactobacillus reuteri protectis (atcc55730) on its ability to improve work-place healthiness by reducing short term sick-leave caused by respiratory or gastrointestinal infections. | 2005 | 16274475 |
| identification and antagonistic activity of lactic acid bacteria occurring in porcine blood from industrial slaughterhouses--a preliminary study. | ninety-seven lactic acid bacteria (lab) were isolated from slaughterhouse porcine blood in order to select autochthonous lab strains for use as biopreservatives of this by-product. they were identified by 16s rdna sequencing; and their inhibition capacity was determined against four bacterial species frequently found in contaminated blood, i.e. staphylococcus aureus, escherichia coli, pseudomonas fluorescens and bacillus spp. the taxonomic study showed an unexpected low diversity of lab in blood ... | 2006 | 16278029 |
| ecological behavior of lactobacillus reuteri 100-23 is affected by mutation of the luxs gene. | the luxs gene of lactobacillus reuteri 100-23c was amplified by pcr, cloned, and then sequenced. to define a physiological and ecological role for the luxs gene in l. reuteri 100-23c, a luxs mutant was constructed by insertional mutagenesis. the luxs mutant did not produce autoinducers ai-2 or ai-3. complementation of the luxs mutation by a plasmid construct containing luxs restored ai-2 and ai-3 synthesis. in vitro experiments revealed that neither the growth rate, nor the cell yield, nor cell ... | 2005 | 16332830 |
| conjugal transfer of broad-host-range plasmid pambeta1 into enteric species of lactic acid bacteria. | the broad-host-range plasmid pambeta1, which codes for erythromycin and lincomycin resistance, was transferred by conjugation into lactobacillus acidophilus, lactobacillus reuteri, and lactobacillus salivarius. a novel 17-megadalton plasmid molecule was detected in the transconjugants, confirming the introduction of pambeta1 into each species. | 1983 | 16346389 |
| production of succinic acid from citric acid and related acids by lactobacillus strains. | a number of lactobacillus strains produced succinic acid in de man-rogosa-sharpe broth to various extents. among 86 fresh isolates from fermented cane molasses in thailand, 30 strains (35%) produced succinic acid; namely, 23 of 39 lactobacillus reuteri strains, 6 of 18 l. cellobiosus strains, and 1 of 6 unidentified strains. all of 10 l. casei subsp. casei strains, 5 l. casei subsp. rhamnosus strains, 6 l. mali strains, and 2 l. buchneri strains did not produce succinic acid. among 58 known stra ... | 1988 | 16347795 |
| purification and characterization of glycerol dehydratase from lactobacillus reuteri. | a coenzyme b(12)-dependent glycerol dehydratase from lactobacillus reuteri has been purified and characterized. the dehydratase has a molecular weight of approximately 200,000, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded a single major band with a molecular weight of 52,000. k(m) values for substrates and coenzyme b(12) were in the millimolar and the submicromolar range, respectively. | 1990 | 16348166 |
| utilization of glycerol as a hydrogen acceptor by lactobacillus reuteri: purification of 1,3-propanediol:nad oxidoreductase. | lactobacillus reuteri utilizes exogenously added glycerol as a hydrogen acceptor during carbohydrate fermentations, resulting in higher growth rates and cell yields than those obtained during growth on carbohydrates alone. glycerol is first converted to 3-hydroxypropionaldehyde by a coenzyme b(12)-dependent glycerol dehydratase and then reduced to 1,3-propanediol by an nad -dependent oxidoreductase. the latter enzyme was purified and determined to have a molecular weight of 180,000; it is predic ... | 1990 | 16348177 |
| probiotics. some evidence of their effectiveness. | to define the term probiotics, to indicate how to identify products that have been proven beneficial, and to assess the quality of evidence regarding probiotics. | 2005 | 16353831 |
| inhibitory effects of lactobacillus reuteri on visceral pain induced by colorectal distension in sprague-dawley rats. | probiotic bacteria are being investigated as possible treatments for many intestinal disorders. the present study aimed to explore the effects of live, heat killed, or gamma irradiated lactobacillus reuteri on cardio-autonomic response and single fibre unit discharge in dorsal root ganglia to colorectal distension in healthy sprague-dawley rats housed under conventional conditions. the effects of this treatment on somatic pain were also examined. | 2006 | 16361309 |
| probiotics to prevent urinary tract infections: the rationale and evidence. | for over 30 years, urologists have recognized in females, that urinary pathogens almost always infect the host through ascension from the rectum, vagina to the urethra and bladder. likewise, the lactobacillus organisms that predominate in the vagina of healthy women, spread from the rectum and perineum and form a barrier in the vagina to bladder entry by uropathogens. the concept of artificially boosting the lactobacilli numbers through probiotic instillation has long been conceived, but only in ... | 2006 | 16389539 |
| a bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium. | a multiple vector system for the production and export of recombinant affinity-tagged proteins in bacillus megaterium was developed. up to 1 mg/liter of a his6-tagged or strep-tagged lactobacillus reuteri levansucrase was directed into the growth medium, using the b. megaterium esterase lipa signal peptide, and recovered by one-step affinity chromatography. | 2006 | 16461726 |
| effect of membrane lateral pressure on the expression of fructosyltransferases in lactobacillus reuteri. | the effect of environmental conditions on the production of homo-polysaccharides and oligosaccharides from sucrose and the regulation of glycosyltransferase genes responsible for biosynthesis of homo-polysaccharides was determined in lactobacillus reuteri tmw1.106 (reutericyclin-producer) and lth5448 (reutericyclin-negative). strain l. reuteri tmw 1.106 harbours the glycosyltransferase genes gtfa and inu, strain lth5448 harbours a fructosyltransferase, ftfa. fructan and fructose-oligosaccharide ... | 2006 | 16464690 |
| antibiotic susceptibility patterns and resistance genes of starter cultures and probiotic bacteria used in food. | a survey of starter and probiotic cultures was carried out to determine the current antibiotic resistance situation in microbial food additives in switzerland. two hundred isolates from 90 different sources were typed by molecular and other methods to belong to the genera lactobacillus (74 samples), staphylococcus (33 samples), bifidobacterium (6 samples), pediococcus (5 samples), or were categorized as lactococci or streptococci (82 samples). they were screened for phenotypic resistances to 20 ... | 2006 | 16464696 |
| new convenient defined media for [(35)s]methionine labelling and proteomic analyses of probiotic lactobacilli. | to develop experimental conditions for efficient protein radiolabelling and two-dimensional (2d) polyacrylamide gel electrophoresis for investigation of stress proteomes of probiotic lactobacillus spp. | 2006 | 16478505 |
| inhibition of expression of a staphylococcal superantigen-like protein by a soluble factor from lactobacillus reuteri. | lactobacillus reuteri rc-14 has previously been shown to inhibit staphylococcus aureus infection in a rat surgical-implant model. to investigate the basis for this, communication events between the two bacterial species were examined. l. reuteri rc-14 and staph. aureus newman were grown in a co-culture apparatus that physically separates the two species, while allowing the passage of soluble compounds. using two-dimensional gel electrophoresis (2d-e), protein expression changes in staph. aureus ... | 2006 | 16549678 |
| the levansucrase and inulosucrase enzymes of lactobacillus reuteri 121 catalyse processive and non-processive transglycosylation reactions. | bacterial fructosyltransferase (ftf) enzymes synthesize fructan polymers from sucrose. ftfs catalyse two different reactions, depending on the nature of the acceptor, resulting in: (i) transglycosylation, when the growing fructan chain (polymerization), or mono- and oligosaccharides (oligosaccharide synthesis), are used as the acceptor substrate; (ii) hydrolysis, when water is used as the acceptor. lactobacillus reuteri 121 levansucrase (lev) and inulosucrase (inu) enzymes are closely related at ... | 2006 | 16549681 |
| [a study of non-antibiotic versus antibiotic prophylaxis for recurrent urinary-tract infections in women (the napruti study)]. | patient enrolment in the 'non-antibiotic versus antibiotic prophylaxis for recurrent urinary-tract infections' (napruti) study was started in september 2005. in this study of women with recurrent urinary-tract infections we aim to investigate the effect of 12 months of non-antibiotic prophylaxis in comparison with antibiotic prophylaxis on the rate of recurrence of urinary-tract infections and the development of antibiotic resistance. the study consists of two interlinked, randomised, clinical n ... | 2006 | 16566424 |
| quantitative real-time pcr analysis of fecal lactobacillus species in infants receiving a prebiotic infant formula. | the developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. generally, bifidobacterium and lactobacillus predominate the microbiota of breast-fed infants. in intervention trials it has been shown that lactobacilli can exert beneficial effects on, for example, diarrhea and atopy. however, the lactobacillus species distribution in breast-fed or formula-fed infants has not yet been determined in gre ... | 2006 | 16597930 |
| effects of a campylobacter jejuni infection on the development of the intestinal microflora of broiler chickens. | the effect of a campylobacter jejuni colonization on the development of the microflora of the cecum and the ileum of broiler chickens was studied using molecular methods. the infection did affect the development and complexity of the microbial communities of the ceca, but we found no permanent effect of a c. jejuni infection on the ileal microflora of the broilers. in addition, denaturant gradient gel electrophoresis (dgge) profiles generated from cecal and ileal contents revealed several dgge b ... | 2006 | 16615340 |