Publications

weissella confusa: problems with identification of an opportunistic pathogen that has been found in fermented foods and proposed as a probiotic.	weissella confusa is found in fermented foods and has been suggested as a probiotic, but also causes sepsis and other serious infections in humans and animals. the incidence of human infections is underestimated partly due to confusion with viridans streptococci and partly due to difficulty making a definitive identification, even if the organism is recognized to belong to another genus, owing to the inability of commercial organism systems to identify it. we report our experiences identifying w ...	2014	24971076
cytotoxicity comparison of quercetin and its metabolites from in vitro fermentation of several gut bacteria.	part of quercetin is coerced into the colon after ingestion and interacts with the gut microbiota. the interaction between quercetin and gut microbiota will influence human health. the cytotoxicity of quercetin and its metabolites from human gut bacteria in vitro fermentation was investigated in this study. the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) (mtt) and agar diffusion disc methods were individually applied in vitro to examine their inhibitory effect on three cultured ...	2014	25015032
effects of ensiling fermentation and aerobic deterioration on the bacterial community in italian ryegrass, guinea grass, and whole-crop maize silages stored at high moisture content.	the effects of storage period and aerobic deterioration on the bacterial community were examined in italian ryegrass (ir), guinea grass (gg), and whole-crop maize (wm) silages. direct-cut forages were stored in a laboratory silo for 3, 7, 14, 28, 56, and 120 d without any additives; live counts, content of fermentation products, and characteristics of the bacterial community were determined. 2,3-butanediol, acetic acid, and lactic acid were the dominant fermentation products in the ir, gg, and w ...	2013	25049913
modeling and investigation of submerged fermentation process to produce extracellular polysaccharide using lactobacillus confusus.	the main objective of the present study is to investigate and optimize the submerged fermentation (smf) process parameters such as addition of coconut water, nacl dose, incubation time and temperature on the production of extracellular polysaccharide (eps) and biomass production using lactobacillus confuses. response surface methodology (rsm) coupled with four factors three level box-behnken design (bbd) was employed to model the smf process. rsm analysis indicated good correspondence between ex ...	2014	25263862
selection of functional lactic acid bacteria as starter cultures for the fermentation of korean leek (allium tuberosum rottler ex sprengel.).	the purpose of this research was to find safe and suitable starter cultures for the fermentation of korean leek (allium tuberosum rottler), also known as garlic chives or oriental garlic. this traditional herb has several functional properties and a strong flavour; its leaves are used as food material. eighteen strains of lactic acid bacteria (lab) were isolated from well-fermented leek kimchi. controlled fermentation of the leek leaves was conducted with 2 strains (weissella confusa lk4 and lac ...	2014	25279760
a confusing case - weissella confusa prosthetic joint infection: a case report and review of the literature.	the authors describe the first case of weissella confusa infection of a prosthetic joint. identification of the pathogen required 16s ribosomal rna sequencing of isolates obtained on two separate occasions during the assessment of an elderly woman with a painful, swollen knee following total knee arthroplasty. a review of reported human infections due to w confusa are summarized, and risk factors and pitfalls in the application of empirical antimicrobial therapy pending definitive microbiologica ...	2014	25285116
unpasteurised commercial boza as a source of microbial diversity.	boza is a cereal-based fermented beverage widely consumed in many countries of the balkans. the aim of this study was to investigate the microbiota of three bulgarian boza samples through a combination of culture-dependent and -independent methods with the long-term objective of formulating a multi-strain starter culture specifically destined for the manufacture of new cereal-based drinks. the isolation campaign for lactic acid bacteria (lab) allowed the identification of lactobacillus parabuchn ...	2015	25437059
cloning and heterologous expression of the ftfcnc-2(1) gene from weissella confusa mbfcnc-2(1) as an extracellular active fructansucrase in bacillus subtilis.	fructan-exopolysaccharides (fructan-eps) (inulin and levan) and their oligosaccharides (fructooligosaccharides, fos) have drawn considerable interest in the food and pharmaceutical industries. eps-producing lactic acid bacteria have been reported to produce β-fructans (inulin and levan), as well as α-glucans, by the function of sucrase enzymes, i.e., fructansucrase and glucansucrase. a fructansucrase ftfcnc-2(1) gene from weissella confusa strain mbfcnc-2(1) was previously cloned in escherichia ...	2015	25454699
characterization of exopolysaccharide and ropy capsular polysaccharide formation by weissella.	with their broad functional properties, lactic acid bacteria derived high molar mass exopolysaccharides (eps) and oligosaccharides are of great interest for food, medical and pharmaceutical industry. eps formation by 123 strains of weissella cibaria and weissella confusa, was evaluated. dextran formation from sucrose was observed for all tested strains while 18 strains produced fructan in addition to dextran. six isolates synthesized a highly ropy polymer from glucose associated with the formati ...	2015	25475311
iranian wheat flours from rural and industrial mills: exploitation of the chemical and technology features, and selection of autochthonous sourdough starters for making breads.	this study aimed at describing the main chemical and technology features of eight iranian wheat flours collected from industrial and artisanal mills. their suitability for bread making was investigated using autochthonous sourdough starters. chemical analyses showed high concentration of fibers and ash, and technology aptitude for making breads. as shown through 2-de analyses, gliadin and glutenin subunits were abundant and varied among the flours. according to the back slopping procedure, type ...	2015	25583343
pyrosequencing vs. culture-dependent approaches to analyze lactic acid bacteria associated to chicha, a traditional maize-based fermented beverage from northwestern argentina.	the diversity of lactic acid bacteria (lab) associated with chicha, a traditional maize-based fermented alcoholic beverage from northwestern argentina, was analyzed using culture-dependent and culture-independent approaches. samples corresponding to 10 production steps were obtained from two local producers at maimará (chicha m) and tumbaya (chicha t). whereas by culture-dependent approach a few number of species (lactobacillus plantarum and weissella viridescens in chicha m, and enterococcus fa ...	2015	25584777
cloning and characterization of a weissella confusa dextransucrase and its application in high fibre baking.	wheat bran offers health benefits as a baking ingredient, but is detrimental to bread textural quality. dextran production by microbial fermentation improves sourdough bread volume and freshness, but extensive acid production during fermentation may negate this effect. enzymatic production of dextran in wheat bran was tested to determine if dextran-containing bran could be used in baking without disrupting bread texture. the weissella confusa vtt e-90392 dextransucrase gene was sequenced and his ...	2015	25603169
genomics of weissella cibaria with an examination of its metabolic traits.	weissella is a genus of lactic acid bacteria (lab) consisting of species formerly included in the leuconostoc paramesenteroides group. similar to other lab, they are commonly found in fermented foods but have also been isolated from environmental and human samples. currently there are 20 recognized species. herein, three weissella cibaria genomes were sequenced using illumia mi-seq and roche 454 technologies. annotation was performed using the prokka and jgi img pipelines. a thorough analysis of ...	2015	25678547
photo quiz: confusing bacteremia in a crohn's disease patient. weissella confusa.		2015	25700525
in vitro modulation of tumor necrosis factor α production in thp-1 cells by lactic acid bacteria isolated from healthy human infants.	the human microbiota is a source of probiotics capable of modulating the host immune system. in this study, we collected fecal samples from 100 healthy infants and isolated lactic acid bacteria which were screened for immune modulating effects on tumor necrosis factor α (tnf-α) production. cell-free culture supernatants from 26 isolates were able to decrease tnf-α production in vitro and three of the isolates were selected as candidate probiotics (msmc39-1, msmc39-3, msmc57-1). these isolates we ...	2015	25759008
construction of a shuttle vector based on the small cryptic plasmid pjy33 from weissella cibaria 33.	a cryptic plasmid, pjy33, from weissella cibaria 33 was characterized. pjy33 was 2365 bp in size with a gc content of 41.27% and contained two putative open reading frames (orfs). orf1 encoded a putative hypothetical protein of 134 amino acids. orf2 was 849 bp in size, and its putative translation product exhibited 87% identity with a replication initiation factor from a plasmid from w. cibaria klc140. a weissella-escherichia coli shuttle vector, pjy33e (6.5 kb, em(r)), was constructed by ligati ...	2015	25882072
epicocconone, a sensitive and specific fluorescent dye for in situ quantification of extracellular proteins within bacterial biofilms.	biofilms are ecosystems of closely associated bacteria encapsulated in an extracellular matrix mainly composed of polysaccharides and proteins. a novel approach was developed for in situ quantification of extracellular proteins (epns) in various bacterial biofilms using epicocconone, a natural, fluorescent compound that binds amine residues of proteins. six commercial proteins were tested for their reaction with epicocconone, and bovine serum albumin (bsa) was selected for assay optimization. th ...	2015	25913004
confused by weissella confusa bacteremia.		2015	25976899
reply to "confused by weissella confusa bacteremia".		2015	25976900
the impact of fermentation with exopolysaccharide producing lactic acid bacteria on rheological, chemical and sensory properties of pureed carrots (daucus carota l.).	fermentation with lactic acid bacteria (lab) offers a natural means to modify technological and nutritional properties of foods and food ingredients. this study explored the impact of fermentation with different exopolysaccharide (eps) producing lab on rheological, chemical and sensory properties of puréed carrots in water, as a vegetable model, with the focus on texture formation. the screening of 37 lab strains for starter selection revealed 16 lactobacillus, leuconostoc and weissella strains ...	2015	26001525
distinctive intestinal lactobacillus communities in 6-month-old infants from rural malawi and southwestern finland.	our aim was to compare the composition and diversity of lactobacillus microbiota in infants living in malawi and southwestern finland.	2015	26049782
lactose- and cellobiose-derived branched trisaccharides and a sucrose-containing trisaccharide produced by acceptor reactions of weissella confusa dextransucrase.	dextran-producing weissella have received significant attention. however, except for maltose, the acceptor reactions of weissella dextransucrases with different sugars have not been investigated. the action of recombinant weissella confusa vtt e-90392 dextransucrase was tested with several potential acceptors, particularly, analogs lactose and cellobiose. the major acceptor products of both disaccharides were identified as branched trisaccharides, with a glucosyl residue α-(1 → 2)-linked to the ...	2016	26212965
exopolysaccharides from co-cultures of weissella confusa 11gu-1 and propionibacterium freudenreichii js15 act synergistically on wheat dough and bread texture.	the storage of bread is limited by both physical (staling) and microbial (mainly fungal) spoilage. exopolysaccharides (eps) produced by lactic acid bacteria (lab) and organic acids from propionibacteria (pab) have been used to enhance texture and extend shelf-life of bakery products. in this study the functionality of eps of weissella confusa a3/2-1 (dextran), w. confusa f3/2-2 (dextran and levan), w. confusa 11gu-1 (dextran and ropy capsular polysaccharide) was evaluated in wheat bread. two str ...	2015	26256717
purification and characterization of bacteriocin produced by weissella confusa a3 of dairy origin.	a dramatic increase in bacterial resistance towards currently available antibiotics has raised worldwide concerns for public health. therefore, antimicrobial peptides (amps) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. the present investigation focusses on the isolation and purification of a novel bacteriocin from an indigenous sample of cow milk and it's mode of action. the bacteriocin was isolated from weissella confusa a3 that was isolated fr ...	2015	26474074
identification of weissella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.	although some weissella species play beneficial roles in food fermentation and in probiotic products, others such as weissella confusa are emerging gram-positive pathogens in immunocompromised hosts. weissella species are difficult to identify by conventional biochemical methods and commercial automated systems and are easily misidentified as lactobacillus and leuconostoc species. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is increasingly being us ...	2015	26594208
screening for antimicrobial and proteolytic activities of lactic acid bacteria isolated from cow, buffalo and goat milk and cheeses marketed in the southeast region of brazil.	lactic acid bacteria (lab) can be isolated from different sources such as milk and cheese, and the lipolytic, proteolytic and glycolytic enzymes of lab are important in cheese preservation and in flavour production. moreover, lab produce several antimicrobial compounds which make these bacteria interesting for food biopreservation. these characteristics stimulate the search of new strains with technological potential. from 156 milk and cheese samples from cow, buffalo and goat, 815 isolates were ...	2016	26608755
rye bran as fermentation matrix boosts in situ dextran production by weissella confusa compared to wheat bran.	the consumption of fiber-rich foods such as cereal bran is highly recommended due to its beneficial health effects. pre-fermentation of bran with lactic acid bacteria can be used to improve the otherwise impaired flavor and textural qualities of bran-rich products. these positive effects are attributed to enzymatic modification of bran components and the production of functional metabolites like organic acids and exopolysaccharides such as dextrans. the aim of this study was to investigate dextr ...	2016	26649737
lactic acid bacteria in dried vegetables and spices.	spices and dried vegetable seasonings are potential sources of bacterial contamination for foods. however, little is known about lactic acid bacteria (lab) in spices and dried vegetables, even though certain lab may cause food spoilage. in this study, we enumerated lab in 104 spices and dried vegetables products aimed for the food manufacturing industry. the products were obtained from a spice wholesaler operating in finland, and were sampled during a one-year period. we picked isolates (n = 343 ...	2016	26678137
quantification of biofilm exopolysaccharides using an in situ assay with periodic acid-schiff reagent.	a novel approach to the quantification of extracellular polysaccharides in miniaturized biofilms presenting a wide variety of extracellular matrices was developed. the assay used the periodic acid-schiff reagent and was first calibrated on dextran and alginate solutions. then it was implemented on 24-h and 48-h biofilms from three strains known to produce different exopolymeric substances (pseudomonas aeruginosa, bacillus licheniformis, weissella confusa). the assay allowed quantification of the ...	2016	26800864
characterization of a novel galactan produced by weissella confusa kr780676 from an acidic fermented food.	an exopolysaccharide (eps) producing strain pufstm055 isolated from idli batter (an indian traditional cereal-legume based fermented food) was identified as weissella confusa kr780676. the strain was shown to produce 17.2g/l (dry weight) of eps in 2% sucrose supplemented mrs broth and the eps was characterized. hptlc analysis confirmed the presence of galactose monomers, indicating the homopolysaccharide nature of eps. fourier-transform infrared spectroscopy and nuclear magnetic resonance analys ...	2016	26836614
ethanol production by selected intestinal microorganisms and lactic acid bacteria growing under different nutritional conditions.	to gain some specific insight into the roles microorganisms might play in non-alcoholic fatty liver disease (nafld), some intestinal and lactic acid bacteria and one yeast (anaerostipes caccae, bacteroides thetaiotaomicron, bifidobacterium longum, enterococcus fecalis, escherichia coli, lactobacillus acidophilus, lactobacillus fermentum, lactobacillus plantarum, weissella confusa, saccharomyces cerevisiae) were characterized by high performance liquid chromatography for production of ethanol whe ...	2016	26858714
structure modeling and functional analysis of recombinant dextransucrase from weissella confusa cab3 expressed in lactococcus lactis.	the dextransucrase gene from weissella confusa cab3, having an open reading frame of 4.2 kb coding for 1,402 amino acids, was amplified, cloned, and expressed in lactococcus lactis. the recombinant dextransucrase, wccab3-rdsr was expressed as extracellular enzyme in m17 medium with a specific activity of 1.5 u/mg which after purification by peg-400 fractionation gave 6.1 u/mg resulting in 4-fold purification. wccab3-rdsr was expressed as soluble and homogeneous protein of molecular mass, approxi ...	2016	26861959
microbial shifts associated with necrotic enteritis.	an outbreak of necrotic enteritis (ne) is a complex process requiring one or a number of predisposing factors rather than just the presence of pathogenic clostridium perfringens. examples are dietary influences, such as high levels of non-starch polysaccharides and fishmeal, and factors that evoke epithelial cell damage, such as fusarium mycotoxins in feed and eimeria infections. recent studies have shown that different predisposing factors induce similar shifts in the intestinal microbiota comp ...	2016	26950294
antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens.	the addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory factor to antibiotic resistance, thus an alternative to antibiotics is needed in animal feed additives.	2015	26957978
identification and sequence analysis of pwcmbf8-1, a bacteriocin-encoding plasmid from the lactic acid bacterium weissella confusa.	members of the gram-positive lactic acid bacteria (lab) are well-known for their beneficial properties as starter cultures and probiotics. many lab species produce ribosomally synthesized proteinaceous antibiotics (bacteriocins). weissella confusa mbf8-1 is a strain isolated from a fermented soybean product that not only produces useful exopolysaccharides but also exhibits bacteriocin activity, which we call weissellicin mbf. here, we show that bacteriocin production by w. confusa mbf8-1 is spec ...	2016	26976853
optimization of isomaltooligosaccharide size distribution by acceptor reaction of weissella confusa dextransucrase and characterization of novel α-(1→2)-branched isomaltooligosaccharides.	long-chain isomaltooligosaccharides (imos) are promising prebiotics. imos were produced by a weissella confusa dextransucrase via maltose acceptor reaction. the inputs of substrates (i.e., sucrose and maltose, 0.15-1 m) and dextransucrase (1-10 u/g sucrose) were used to control imo yield and profile. according to response surface modeling, 1 m sucrose and 0.5 m maltose were optimal for the synthesis of longer imos, whereas the dextransucrase dosage showed no significant effect. in addition to th ...	2016	27050481
community dynamics and metabolite target analysis of spontaneous, backslopped barley sourdough fermentations under laboratory and bakery conditions.	barley flour is not commonly used for baking because of its negative effects on bread dough rheology and loaf volume. however, barley sourdoughs are promising ingredients to produce improved barley-based breads. spontaneous barley sourdough fermentations were performed through backslopping (every 24h, 10days) under laboratory (fermentors, controlled temperature of 30°c, high dough yield of 400) and bakery conditions (open vessels, ambient temperature of 17-22°c, low dough yield of 200), making u ...	2016	27088869
probiotic properties of lactic acid bacteria isolated from human milk.	the objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties.	2016	27159339
molecular cloning of kman coding for mannanase from klebsiella oxytoca kub-cw2-3 and its hybrid mannanase characters.	gene encoding for β-mannanase (e.c 3.2.1.78) from klebsiella oxytoca kub-cw2-3 was cloned and expressed by an e. coli system resulting in 400 times higher mannanase activities than the wild type. a 3314bp dna fragment obtained revealed an open reading frame of 1164bp, namely kman-2, which encoded for 387 amino acids with an estimated molecular weight of 43.2kda. it belonged to the glycosyl hydrolase family 26 (gh26) exhibited low similarity of 50-71% to β-mannanase produced by other microbial so ...	2016	27233126
intestinal lactobacillus community structure and its correlation with diet of southern chinese elderly subjects.	this study aimed to investigate the relationship between the intestinal lactobacillus species and diet of elderly subjects in a longevity area in southern china. healthy elderly subjects ranging from 80 to 99 years old were respectively selected from the regions of bama and nanning, guangxi, china. the nested polymerase chain reaction and denaturing gradient gel electrophoresis (pcr-dgge) technology was used to analyze the intestinal lactobacillus community structure. results showed that weissel ...	2016	27572508
physico-chemical characterization of galactan exopolysaccharide produced by weissella confusa kr780676.	physico-chemical properties of a galactan exopolysaccharide (eps) produced by weissella confusa kr780676 isolated from an idli batter were studied. in our previous paper, we had reported the isolation and characterization of a linear galactan (containing α-(1→6)-linked galactose units) isolated from an acidic fermented food. in this manuscript, we are reporting the physico-chemical properties viz., colour evaluation (l* 81.79, a* 3.15, b* 15.04), flow properties (compressibility index 38.46%), s ...	2016	27645923
natural lactic acid bacteria population of tropical grasses and their fermentation factor analysis of silage prepared with cellulase and inoculant.	natural lactic acid bacteria (lab) populations in tropical grasses and their fermentation characteristics on silage prepared with cellulase enzyme and lab inoculants were studied. a commercial inoculant lactobacillus plantarum chikuso 1 (ch), a local selected strain lactobacillus casei th14 (th14), and 2 cellulases, acremonium cellulase (ac) and maicelase (mc; meiji seika pharma co. ltd., tokyo, japan), were used as additives to silage preparation with fresh and wilted (6 h) guinea grass and nap ...	2016	27743669
draft genome sequence of weissella confusa mbf8-1, a glucansucrase- and bacteriocin-producing strain isolated from a homemade soy product.	we report here the draft genome sequence of weissella confusa mbf8-1, an isolate from a homemade fermented soybean product that produces sucrases and exhibits antibacterial (bacteriocin) activity. the draft genome of w. confusa mbf8-1 comprises a 2.2-mbp chromosome and a 17.8-kbp bacteriocin-encoding plasmid. two putative glucansucrase genes were also identified.	2017	28126938
[weissella confusa bacteremia: an underestimated opportunistic pathogen].		2017	28325577
dynamics of microbial community during ensiling direct-cut alfalfa with and without lab inoculant and sugar.	to gain deeper insights into the clostridial community dynamics and chemical transformations during the ensiling of alfalfa.	2017	28370869