Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the influence of sucralose on bacterial metabolism. | sucralose (1',4',6' trideoxy-trichloro-galactosucrose) is a nontoxic, intensely sweet sucrose derivative that has been shown to be non-cariogenic in experimental animals. the purpose of this study was to determine whether certain oral bacteria could utilize sucralose. sucralose, as a sole carbon source, was unable to support growth of ten strains of oral bacteria and dental plaque. when sucrolose was incorporated into a liquid medium containing glucose or sucrose, all organisms tested displayed ... | 1990 | 2143512 |
| characterization of the product of the gtfs gene of streptococcus downei, a primer-independent enzyme synthesizing oligo-isomaltosaccharides. | the gtfs gene, coding for a glucosyltransferase which synthesizes water-soluble glucan and previously cloned from streptococcus downei strain mfe28 (mutans serotype h) into a bacteriophage vector, was subcloned into a plasmid vector. the gtfs gene products expressed in escherichia coli were compared to the primer-independent, oligo-isomaltosaccharide synthase in streptococcus sobrinus strain aht (mutans serotype g) and shown to resemble it closely in molecular mass, isoelectric point, immunologi ... | 1990 | 2148181 |
| effect of local deposition of antigen on salivary immune responses and reaccumulation of mutans streptococci. | in this study glucosyltransferase (gtf) from streptococcus sobrinus was topically administered onto the lower lips of young adults. the effect of this route of antigenic exposure on labial and parotid gland and serum antibody levels to gtf and on the reaccumulation of indigenous mutans streptococci after a dental prophylaxis was then measured. young adults between 18 and 42 years of age were screened for levels of antibody activity to gtf in parotid and labial gland salivas and levels of mutans ... | 1990 | 2148321 |
| interaction of structural isomers of sucrose in the reaction between sucrose and glucosyltransferases from mutans streptococci. | structural isomers of sucrose, i.e. disaccharides composed of glucose and fructose molecules with different glucosidic linkages, were examined for their effect on the reaction between sucrose and various glucosyltransferases (gtases) from streptococcus mutans mt8148 and streptococcus sobrinus 6715. trehalulose (alpha 1-1), turanose (alpha 1-3), maltulose (alpha 1-4), and palatinose (alpha 1-6) were used as the sucrose analogues. mutans streptococci were found not to utilize these sucrose analogu ... | 1990 | 2150553 |
| glucosyltransferase production by streptococcus sanguis challis and comparison with other oral streptococci. | glucosyltransferase (gtf) activity in batch cultures of streptococcus sanguis strain challis in defined medium was maximum at peak growth and declined rapidly on further incubation into stationary phase. activity was present in spent culture medium and was associated with 2 polypeptides of approximate mrs 170 kda (90% of activity) and 155 kda (about 10% of activity). other s. sanguis strains produced similar antigenically-related polypeptides but with varying activities, and antibodies to the s. ... | 1990 | 2150879 |
| phenytoin-induced gingival overgrowth in rats infected with streptococcus sobrinus 6715. | inbred fischer rats were fed a high sucrose diet (no. 2000) containing phenytoin (5,5-diphenyl-hydantoin) for 52 days, which produced serum and salivary pht levels of 13-14 and 2.6-2.7 micrograms/ml, respectively. gingival overgrowth was induced in the molar region of all pht-treated rats but was more severe in streptococcus sobrinus 6715-infected rats than in non-infected rats, which had lower plaque scores. no significant overgrowth was found in rats infected with strep. sobrinus and fed the s ... | 1990 | 2151111 |
| bicarbonate-based powder and paste dentifrice effects on caries. | the effects of sodium bicarbonate-based powder and paste dentifrices on dental caries and streptococcus sobrinus recoveries in rats were studied. three s. sobrinus 6715-13wt infected groups of specific pathogen-free weanling rats, consuming a diet which contains 56% sucrose, were topically treated with either demineralized water, dentifrice paste, or dentifrice powder. both the paste and powder contained 0.22% (w/w) naf. all topical treatments were given for one min daily per rat, five days a we ... | 1990 | 2165447 |
| proteolytic activity of oral streptococci. | streptococcus mutans and streptococcus sobrinus were the least proteolytic of 8 species of oral streptococci while streptococcus oralis and streptococcus sanguis were the most proteolytic. degradation of fitc-bsa was significantly correlated with the hydrolysis of synthetic endopeptidase substrates. as s. oralis strains proliferate in dental plaque in the absence of dietary food their success, in vivo, might be due partially to their greater proteolytic activity compared to other oral streptococ ... | 1990 | 2182385 |
| [demonstration of streptococcus sobrinus in human oral cavity]. | from 133 saliva samples of humans we isolated 32 strains (24%) of streptococcus sobrinus on an bycsb selective medium. the very hard colonies of s. sobrinus on this medium were characterized by a distinct chalky white halo of water insoluble glucan ("glucan-halo") produced in large amounts from the sucrose by extracellular glucosyltransferase which has diffused out into the isolation medium. all of the isolated s. sobrinus strains fermented ordinarily mannitol and sorbitol, but not raffinose and ... | 1990 | 2206607 |
| prevalence of streptococcus sobrinus in relation to dental caries in children from iceland and the netherlands. | the aim of this study was to investigate the presence of s. sobrinus and s. mutans in specimens of dental plaque and saliva of children five years of age in reykjavik, iceland (study 1) and in samples of dental plaque from children nine years of age in amsterdam, the netherlands (study 2). the immuneblotting technique (ibt) was a suitable method to evaluate the presence and numbers of s. mutans and s. sobrinus in human dental plaque and saliva. in study 1, eighty-four children were evaluated bac ... | 1990 | 2212191 |
| molecular cloning and characterization of the spab gene of streptococcus sobrinus. | a gene of streptococcus sobrinus 6715 (serotype g) designated spab and encoding a surface protein antigen was isolated from a cosmid gene bank. a 5.4 kb hindiii/avai dna fragment containing the gene was inserted into plasmid pbr322 to yield plasmid pxi404. analysis of plasmid-encoded gene products showed that the 5.4 kb fragment of pxi404 encoded a 195 kda protein. southern blot experiments revealed that the 5.4 kb chromosomal insert dna had sequence similarity with genomic dna of s. sobrinus 67 ... | 1990 | 2230719 |
| arginine catabolism by strains of oral streptococci. | arginine catabolism via the arginine deiminase pathway was found in streptococcus sanguis 903. citrulline and ornithine were released from resting cells incubated with arginine, arginine-containing peptides, or saliva. maximum arginine catabolism by resting cells of s. sanguis 903 was found in the ph range 7-8 and at 45-48 degrees c. arginine deiminase activity was found in the cytoplasm and in the cell-wall extract of this strain, while ornithine carbamoyltransferase activity was found in the c ... | 1990 | 2248769 |
| enumeration of mutans streptococci in clinical samples by using monoclonal antibodies. | mutans streptococci have been strongly associated with dental caries. two members of this group of bacteria, streptococcus mutans and streptococcus sobrinus, are often found in human dental plaque. identification of mutans streptococci on the basis of sugar fermentation is troublesome and easily leads to erroneous conclusions. furthermore, the recovery on selective media differs for different species. this causes incorrect enumeration of s. mutans and s. sobrinus in clinical samples. the aim of ... | 1990 | 2254421 |
| caries incidence in intact rats infected with streptococcus sobrinus via transmission from desalivated cagemates. | the purpose of this study was to determine the influence of caging desalivated s. sobrinus-infected rats on the caries incidence in intact, previously uninfected cagemates. weanlings were divided as follows: stage i: twenty desalivated animals paired with 20 intact animals (group i); 20 intact animals paired with 20 intact animals (group ii). animals were infected with s. sobrinus and fed diet 2000 and 10% sucrose water. after five weeks, ten paired animals from each group were killed. desalivat ... | 1990 | 2335648 |
| antiplaque activity of some antimicrobial agents using a simple in vitro method. | some antimicrobial compounds were tested for their antiplaque activity by a simple method involving measurement of the weight of streptococcus sobrinus plaque on a glass surface. chlorhexidine, cetylpyridinium chloride, and n-tetradecylamine reduced the weight of plaque produced by s. sobrinus in vitro; some n-palmityl and palmitoyl derivatives of polymethylenediamines were also found to possess antiplaque activity. the results suggest that an antiplaque effect does not necessarily depend on hig ... | 1990 | 2340540 |
| salivary conditions and cariogenic microorganisms in 55, 65, and 75-year-old swedish individuals. | secretion rate, buffer capacity, and cariogenic microorganisms of resting and stimulated whole saliva were examined in 208 55-, 65-, and 75-yr-old swedes. the secretion rate for both resting and stimulated saliva decreased with age. when the use of drugs was taken into account the difference in terms of age group was significant for resting saliva alone (p less than 0.01). in 22% the resting saliva was less than 0.1 ml/min, and in 5% the stimulated saliva was less than 0.7 ml/min. persons with s ... | 1990 | 2349449 |
| in vitro screening of chinese medicinal toothpastes: their effects on growth and plaque formation of mutans streptococci. | many plant extracts or derivatives have been incorporated into commercial toothpastes to treat oral diseases related to caries or periodontal diseases in china. however, no information is available concerning their in vitro effects on oral bacteria. thirty-one chinese medicinal toothpastes were selected for this study. their ability to inhibit growth, in vitro plaque formation and glucosyltransferase (gtf) activity of streptococcus sobrinus b13 and streptococcus mutans 3209 were examined. eighty ... | 1990 | 2364405 |
| gingival overgrowth induced by cyclosporin a in rats. | gingival overgrowth, which is one of the major side-effects of this immunosuppressive agent, was studied in specific pathogen-free fischer rats fed diet no. 2000 containing 56% sucrose. marked macroscopic overgrowth was noted in mandibular gingiva of all the rats fed this diet containing cyclosporin a. the overgrowth was more severe in buccal than in lingual gingiva. rats fed the cyclosporin-containing diet and infected with streptococcus sobrinus 6715 had the most gingival overgrowth. histopath ... | 1990 | 2372253 |
| strains of streptococcus mutans and streptococcus sobrinus attach to different pellicle receptors. | we compared the levels of adsorption of streptococcus mutans jbp and streptococcus sobrinus 6715 to experimental pellicles formed from unsupplemented and glucosyltransferase (gtf)-supplemented saliva. pellicles formed on hydroxyapatite beads from gtf or from saliva-gtf mixtures possessed detectable gtf activity. low levels of gtf activity were also detected in clarified whole human saliva, but not in samples of submandibular saliva. the adsorptive behavior of s. mutans jbp to pellicles formed fr ... | 1986 | 2422126 |
| renaturation of dextranase activity from culture supernatant fluids of streptococcus sobrinus after sodium dodecylsulfate polyacrylamide gel electrophoresis. | a rapid and reproducible method for the assay of individual fractions of the multicomponent dextranase activity of streptococcus sobrinus after reduction/denaturation and electrophoresis in acrylamide gels is described. multiple forms of dextranase, possible virulence factors in the formation of dental caries by oral streptococci (s. mutans, s. sobrinus, s. sanguis, s. cricetus, and s. rattus), have been separated in sodium dodecylsulfate-polyacrylamide gels into which an indicator substrate, bl ... | 1986 | 2433959 |
| characterization by affinity electrophoresis of an alpha-1,6-glucan-binding protein from streptococcus sobrinus. | glucan-binding protein 1 (gbp1), the most abundant glucan-binding protein isolated from culture supernatants of streptococcus sobrinus 6715-49, has been purified by affinity chromatography on sephadex g-50 followed by gel permeation chromatography with bio-gel p-10. the specificity and affinity of gbp1 for glucans were assessed by affinity electrophoresis. gbp1 did not detectably bind to glucans lacking linear arrays of alpha-1,6 linkages. the association constant for the linear alpha-1,6-glucan ... | 1987 | 2445685 |
| genetic characterization of the spaa1 determinant of the surface protein antigen gene from streptococcus sobrinus 6715. | 1. the surface protein antigen-encoding gene spaa1, previously cloned from streptococcus sobrinus 6715 (serotype g) into the plasmid pacyc184, was examined. 2. the gene product of pya726 was identified both by the minicell method and by in vitro transcription-translation system as a protein whose mol. wt was estimated to be 140,000. 3. the spaa1 gene was localized on the 3.75 kb avai-bamhi fragment of the cloned dna. 4. an internal spaa1 promoter located close to one end of the 1.25 kb avai frag ... | 1989 | 2482205 |
| [changes of antibody and splenic plaque forming cell responses to mutans streptococci in the rats infected with s. sobrinus 6715]. | the study was undertaken to examine the changes in humoral igm, igg and iga antibodies and splenic plaque forming cell responses against streptococcus sobrinus 6715 in rats infected with the same mutans streptococci. the serum samples were collected every week for 24 weeks after infection (20 day old, sprague dawley rats). humoral anti-s. sobrinus 6715 igg and iga antibodies were determined by an enzyme-linked immunosorbent assay. in the separate experiment, rat splenic igm, igg and iga anti-tri ... | 1989 | 2489371 |
| [caries-inducing activity of isomaltooligosugar (imos) in in vitro and rat experiments]. | the caries-inducing activity of isomaltooligosugar (imos: a isomaltose rich sugar mixture) was examined in in vitro and in vivo experiments. strains of streptococcus mutans mt8148r and streptococcus sobrinus 6715 fermented imos and produced acids. imos inhibited the glucan synthesis from sucrose by glucosyltransferases from both of s. mutans and s. sobrinus. furthermore, imos inhibited sucrose-dependent adherence of those mutans streptococci. imos was found to induce significant but minimal cari ... | 1989 | 2489886 |
| sucrose 6-alpha-d-glucosyltransferase from streptococcus sobrinus: characterization of a glucosyl-enzyme complex. | a covalent glucosyl-enzyme was isolated from a quenched reaction of streptococcus sobrinus sucrose 6-alpha-d-glucosyltransferase and radiolabeled sucrose. no complex was observed with heat-inactivated enzyme or when sucrose was replaced with radiolabeled maltose or glucose. the complex was stable at ph 2 in 1% sodium dodecyl sulfate, 6.0 m urea, and 4.0 m guanidine hydrochloride, but became increasingly labile with increased ph (32-min half-life at ph 7.0). d-glucose was the exclusive radiolabel ... | 1989 | 2523727 |
| use of 13c-n.m.r. spectroscopy for the quantitative estimation of 3-o- and 3,6-di-o-substituted d-glucopyranosyl residues in alpha-d-glucans formed by the d-glucosyltransferases of streptococcus sobrinus. | the 13c-n.m.r. spectra of the three alpha-d-glucans from streptococcus sobrinus and the dextran from leuconostoc mesenteroides, which differ widely in the ratios of omega (terminal, nonreducing) d-glucopyranosyl groups: 3-:6-:3,6-linked d-glucopyranosyl (glc) residues, were measured in 0.5m naoh at 22 degrees. the c-1 signals of 3-o-substituted glc in a linear sequence, 6-o-substituted glc in a linear sequence, 3,6-di-o-substituted glc in a (1----6)-linked sequence, and glc attached to o-3 of 3, ... | 1989 | 2525070 |
| glucan-binding domain of a glucosyltransferase from streptococcus sobrinus: isolation of a 55-kilodalton peptide from a trypsin digest of glucosyltransferase prebound to insoluble glucan. | we isolated a glucan-binding domain of water-insoluble glucan synthase (gtf-i) of streptococcus sobrinus b13. mild trypsin digestion of gtf-i bound to a water-insoluble glucan (ig) produced one predominant large fragment (55 kilodaltons). the fragment was easily recovered in ig precipitate. the isolated fragment had the same degree of affinity to ig as did the native gtf-i but no glucan synthesis activity. by the same method, a similar 55-kilodalton fragment was protected for gtf-sd but not for ... | 1989 | 2525114 |
| inhibition by maltose, isomaltose, and nigerose of the synthesis of high-molecular-weight d-glucans by the d-glucosyltransferases of streptococcus sobrinus. | two d-glucosyltransferases are produced by streptococcus sobrinus c211. one (gtf-s) catalyzes the conversion of sucrose into soluble alpha-(1----6)-linked alpha-(1----3)-branched d-glucans, and the other (gtf-i), of sucrose into alpha-(1----3)-linked alpha-(1----6)-branched d-glucans. these enzymes were studied by using maltose, isomaltose, and nigerose as inhibitors. maltose and isomaltose were found to be competitive inhibitors of gtf-s, whereas nigerose has no effect on gtf-s activity. the ki ... | 1989 | 2526680 |
| purification of a fourth glucosyltransferase from streptococcus sobrinus. | recently, we found a novel primer-independent, water-soluble glucan synthase as a fourth glucosyltransferase (gtf) in a culture supernatant of strain aht-k of streptococcus sobrinus (y. yamashita, n. hanada, and t. takehara, biochem. biophys. res. commun. 150:687-693, 1988). in the present study, four kinds of purified gtfs, including the novel gtf, were prepared. they were composed of two primer-dependent gtfs and two primer-independent gtfs. of the primer-dependent gtfs, one was a water-insolu ... | 1989 | 2530209 |
| effect of magnesium ions on secretion of glucosyltransferase from streptococcus sobrinus. | magnesium ions, which are known to inhibit the leader peptidase (signal peptidase) activity of escherichia coli, stimulated the activities of glucosyltransferases (gtfs) in culture medium of streptococcus sobrinus at concentrations of 1 or 5 mm. at a concentration of 50 mm, the activity of water-soluble glucan synthase was not affected and that of water-insoluble glucan synthase was slightly suppressed. the activities of both gtfs in the crude enzyme extract were totally unaffected by addition o ... | 1989 | 2533315 |
| glucosyltransferases of streptococcus sobrinus c211 are both stimulated and inhibited by hydrogen peroxide. | there are 2 glucosyltransferases (gtf) produced by streptococcus sobrinus c211. one enzyme, gtf-s, produces a water-soluble glucan that is a-1,6-linked, with short a-1,3 branches, and the other enzyme, gtf-i, produces a water-insoluble glucan that is a-1,3-linked with a-1,6 branches. hydrogen peroxide was found not only to be a potent inhibitor of gtf activity, but also a stimulator of gtf activity when employed at relatively low concentrations. at 0.88 m, h2o2 completely inhibited insoluble glu ... | 1989 | 2534763 |
| effect of several resin monomers on water insoluble glucan formation by glucosyltransferase of streptococcus sobrinus. | the cause or mechanism by which more plaque tend to accumulate on composite resins than on any other restorative materials has yet to be sufficiently explained. the purpose of this study was to investigate a possible mechanism by clarifying the effects of several monomers on the glucosyltransferase activity from streptococcus sobrinus. the following results were obtained. in the individual resin monomers, di-, tri- and tetra-ethylene glycol dimethacrylate (2g, 3g and 4g), bis-gma and udma showed ... | 1989 | 2535160 |
| inhibitory effects of humic acid on insoluble glucan synthesis and cellular adherence of streptococcus mutans (sobrinus) 6715. | 1989 | 2535262 | |
| molecular and immunochemical characterization of recombinant escherichia coli containing the spaa gene region of streptococcus sobrinus. | we identified and characterized a recombinant escherichia coli containing the entire gene for surface protein antigen a (spaa) of streptococcus sobrinus 6715. the recombinant e. coli was isolated from a cosmid gene bank of size-fractionated s. sobrinus dna fragments, and recombinants expressing the spaa protein were detected immunologically. subcloning experiments showed that the dna sequences encoding the spaa protein could be isolated on two contiguous ecori fragments, 3.7 and 3.3 kilobases (k ... | 1989 | 2543627 |
| isolation and characterization of the streptococcus mutans gtfd gene, coding for primer-dependent soluble glucan synthesis. | two glucosyltransferase genes from streptococcus mutans gs-5, gtfb and gtfc, have been previously isolated and sequenced in this laboratory. in the present communication a third gtf gene, gtfd, was isolated and characterized. isolation of the gene involved a novel procedure utilizing the integration plasmid pva891. a peptide expressed by the 1.7-kilobase dna fragment from strain nhs1 (containing deletions in both the gtfb and gtfc genes) was initially identified in a puc18 clone bank with antigl ... | 1989 | 2543630 |
| dna homology of surface protein antigen a gene in mutans streptococci. | 1. a recombinant plasmid, pya724, containing an 8.45 kb dna fragment encoding surface protein antigen a (spaa) from streptococcus sobrinus 6715 was used to examine the dna homology of the spaa gene with chromosomal dna of various mutans streptococci strains. 2. restriction endonuclease bamhi-digested pya724 dna was radio-labeled by nick-translation, and a dna-dna hybridization experiment was carried out. pya724 dna hybridized with chromosomal dna of serotypes a, c, d, e, f and g strains, but not ... | 1989 | 2591635 |
| the influence of saliva on infection of the human mouth by mutans streptococci. | the relationship between oral implantation of streptococcus mutans ib1600 (serotype c) and streptococcus sobrinus omz65 (serotype g), the aggregating activity of saliva, and its influence on the adherence of these bacterial strains in vitro was examined in seven human subjects. all the saliva samples aggregated strain ib1600 but not strain omz65 cells. whole saliva from subjects with low levels of infection by strep. mutans aggregated strain ib1600 to a greater degree than did whole saliva from ... | 1989 | 2597028 |
| cloning of the gene for cell-surface protein antigen a from streptococcus sobrinus (serotype d). | a gene library of strep. sobrinus b13n (serotype d) chromosomal dna was constructed in escherichia coli, with the bacteriophage vector lambda l47.1. a recombinant phage, lambda mdsm49, containing a 15.5 kb dna insert, directed the expression of a 210 kda antigenic protein. the recombinant 210 kda protein was shown by western blot analysis to be identical with cell-surface protein antigen a (spaa) from a serotype g strain. however, the restriction patterns of a subclone plasmid, pmd51, from lambd ... | 1989 | 2597046 |
| caries on root surfaces exposed following gingivectomy in conventional rats infected with streptococcus sobrinus and actinomyces viscosus. | to study the ability of bacteria associated with coronal caries to initiate root surface caries, a rat model was used. root surfaces were exposed by gingivectomy in rats fed a caries-promoting diet and orally inoculated with either actinomyces viscosus m-100, streptococcus sobrinus (mutans) 6715, or both. a fourth group received a diet containing antibiotics. the animals were sacrificed 64 days following the gingivectomy performed on the right molar quadrants. gingivectomy significantly increase ... | 1989 | 2600749 |
| comparative cariogenicity and dental plaque-forming ability in gnotobiotic rats of four species of mutans streptococci. | the four species, each represented by two strains, all produced high levels of fissure caries in the upper and lower jaws of gnotobiotic fisher rats. streptococcus rattus strains and streptococcus mutans nctc 10832 gave low levels of approximal caries, whereas streptococcus cricetus, streptococcus sobrinus and strep. mutans nctc 10449 gave high levels. the only strains to produce buccal surface lesions were strep. sobrinus and strep. cricetus. all strains were able to form dense plaque in the ce ... | 1989 | 2610617 |
| diversity of surface protein antigen a gene in the chromosomal dna of streptococcus sobrinus. | a recombinant plasmid, pya724, containing an 8.45-kb dna fragment encoding surface protein antigen a (spaa) from streptococcus sobrinus 6715 was used to examine the dna homology of the spaa gene with chromosomal dna of streptococcus sobrinus b13n. restriction endonuclease bamhi-digested pya724 dna was 32p radio-labeled by nick-translation, and a southern blot dna-dna hybridization experiment was carried out. pya724 dna hybridized with a bamhi fragment of b13n chromosomal dna, and the size of thi ... | 1989 | 2626165 |
| associations between salivary levels of streptococcus mutans, streptococcus sobrinus, lactobacilli, and caries experience in kenyan adolescents. | salivary levels of mutans streptococci (s. mutans and s. sobrinus) and lactobacilli were determined in a random sample of rural kenyans between 15 and 19 years of age (n = 149). it is possible for the natural history of dental caries in this population to be studied since it is characterized by a limited access to conventional dental treatment. using a short set of biochemical tests, we identified from seven to ten presumptive mutans streptococcus colonies--cultured from the saliva of each indiv ... | 1989 | 2632612 |
| uptake of 14c-xylitol by xylitol-cultured streptococcus sobrinus atcc 27352 and streptococcus mitis atcc 36249 in vitro. | the effect of three successive cultures in the presence of 6% xylitol on the uptake of 14c-xylitol was studied using resting cells of streptococcus sobrinus atcc 27352 and streptococcus mitis atcc 36249. in the case of s. mitis, the three successive cultures did not alter the growth inhibition observed in the presence of xylitol. in the case of s. sobrinus, however, growth inhibition decreased. the 14c-xylitol uptake experiments also demonstrated that uptake of xylitol by s. sobrinus was decreas ... | 1989 | 2633204 |
| characterisation of monoclonal antibodies to common protein epitopes on the cell surface of streptococcus mutans and streptococcus sobrinus. | three monoclonal antibodies (mab) were prepared against a cell surface antigen which cross-react between streptococcus mutans (serotypes c, e and f) and streptococcus sobrinus (serotypes d and g). two of the mab also recognise a determinant on the surface of streptococcus cricetus (serotype a). the common antigen shared between s. mutans and s. sobrinus was demonstrated by western blotting to be about 200 kd in size. this antigen is shared not only by the cell surfaces of serotypes a, c, d, e, f ... | 1989 | 2639299 |
| salivary levels of putative cariogenic organisms in patients with eating disorders. | the present study examined the hypothesis that women with eating disorders associated with a history of chronic vomiting can be characterized by a salivary flora with high levels of aciduric organisms, such as, mutans streptococci, lactobacilli and yeast. three groups of female subjects were studied: vomiting bulimics (g1; n = 14), and comparison groups selected for high streptococcus mutans (g2; n = 13), and low s. mutans levels (g3; n = 12). the prevalence and levels of mutans streptococci, la ... | 1989 | 2640318 |
| homology between surface protein antigen genes of streptococcus sobrinus and streptococcus mutans. | the structural gene (pag gene) for a 210 kda protein antigen of streptococcus sobrinus serotype g was cloned and compared with that (pac gene) of a 190 kda protein antigen of streptococcus mutans serotype c. immunodiffusion analysis revealed that the product of the pag gene immunologically cross-reacted with that of the pac gene. southern blot and nucleotide sequence analyses revealed that a significant homology existed between the middle regions of the two structural genes. | 1989 | 2661267 |
| effect of desalivation and age on susceptibility to infection by streptococcus sobrinus. | the influence of desalivation and age on susceptibility to infection by streptococcus sobrinus was studied using 80 sprague-dawley female rats weaned at age 21 days. forty animals (donors) were infected with s. sobrinus 6715 (atcc 27352), formed into ten groups of 4, fed diet 2000, and offered 10% sucrose water ad libitum. the remaining 40 animals were fed laboratory chow and sterile distilled water until introduced into the experiment. when the animals were aged 25 days, 10 noninfected animals ... | 1989 | 2743376 |
| molecular characterization of a surface protein antigen gene from serotype c streptococcus mutans, implicated in dental caries. | the complete nucleotide sequence of the gene for a cell-surface protein antigen (pac) of streptococcus mutans mt8148 (serotype c) was determined. the pac gene consisted of 4695 bp and coded for a 170773d protein. the pac gene product contained a putative 38 amino acid signal peptide, resulting in a 166817d mature protein. a potential promoter sequence and a putative shine-dalgarno sequence preceded the open reading frame. two internal repeating amino acid sequences were present in the pac. one r ... | 1989 | 2761390 |
| effect of a bacteriocin-producing strain of streptococcus sobrinus on infection and establishment of streptococcus mutans on tooth surfaces in rats. | the effect of bacteriocin produced by streptococcus sobrinus mt6223 on infection and establishment of streptococcus mutans mt6222 was studied in specific pathogen-free rats. these strains were isolated from a carious lesion of a single subject. s. mutans mt6222 was found to be susceptible to the growth inhibitory action of s. sobrinus mt6223. when simultaneously inoculated into the oral cavity of rats, even a small inoculum (10(5) cfu) of s. sobrinus mt6223 completely inhibited colonization of s ... | 1989 | 2762016 |
| ultrastructural changes in streptococcus sobrinus induced by xylitol, naf and zncl2. | the effect of xylitol, naf, or zncl2 on the ultrastructure of streptococcus sobrinus omz 176 was studied. the bacteria were grown statically in air for 8 h in brain-heart infusion broth supplemented with 33 mm xylitol, 2.5 mm naf, 0.5 mm zncl2, separately or in combination. the bacteria were washed briefly and prepared for scanning and transmission electron microscopy. all three agents and their combinations induced distinct ultrastructural alterations. the alterations were related to various as ... | 1989 | 2766317 |
| prevention of rat fissure caries by sodium fluoride varnish (duraphat) with different fluoride concentrations. | 125 osborne-mendel rats were weaned at 22-23 days, inoculated with streptococcus sobrinus and fed a cariogenic diet for 40 days. the control group received no treatment; the study groups received applications of duraphat containing 2.3, 1.1, or 0.6% fluoride or a placebo varnish on days 21-23. the placebo varnish had no effect on caries. fissure caries was significantly reduced by the 2.3 and 1.1% fluoride varnishes, whereas the caries reduction found after treatment with the 0.6% fluoride varni ... | 1989 | 2766323 |
| plaque inhibition by a combination of zinc citrate and sodium lauryl sulfate. | bacteriological tests demonstrated an additive inhibitory effect of zncl2 and sodium lauryl sulfate (sls) on in vitro growth of streptococcus sobrinus omz 176 and of streptococcus sanguis atcc 10556. as measured by atomic absorption spectrophotometry, the solubility of zinc citrate increased in the presence of sls. after 48 h, the concentration of solubilized zinc from aqueous solutions of 5.0 mm zinc citrate was 12.0 mm versus 14.4 mm in the presence of 34.7 mm sls. the plaque-inhibiting proper ... | 1989 | 2790864 |
| two functionally different glucose phosphotransferase transport systems in streptococcus mutans and streptococcus sobrinus. | 1987 | 2853322 | |
| acidogenesis by oral streptococci at different ph values. | streptococcus mutans is known to be one of the most cariogenic oral microorganisms. recently, organisms classified as s. mutans have been shown to be genotypically heterogeneous and to form at least six separate species. in this study, differences in acid production between the mutans streptococci and streptococcus sanguis were investigated at ph values from 5.0 to 7.0. while all of the species investigated were able to produce acids, streptococcus sobrinus was found to be significantly more aci ... | 1989 | 2920379 |
| protection by f, i, sr, and combinations against fermentation attack by streptococcus sobrinus artificial plaque on bovine enamel. | labial surfaces of 64 bovine incisors (8 teeth/treatment) were subjected to 1-min treatment with (1) 500 ppm sr, (2) 0.5% i2 plus 1% ki solution, (3) f varnish treatment (duraphat) for 24 h, or (4) combined treatments. the treated teeth were incubated under an artificial streptococcus sobrinus plaque for 10 days. the 'oral fluid' with maleate buffer (ph 5.8) partially saturated with ca3(po4)2 and with or without 3.3% sucrose or sucrose plus 25 ppm f, was replaced by a mixture containing thioglyc ... | 1989 | 2920385 |
| comparison of extracellular protein profiles of seven serotypes of mutans streptococci grown under controlled conditions. | extracellular proteins produced by the four human commensal species of mutans streptococci were analysed. the organisms used were streptococcus mutans, serotypes c, e and f, streptococcus cricetus, serotype a, streptococcus rattus, serotype b, and streptococcus sobrinus, serotypes d and g. they were grown in continuous culture at different generation times and ph values in media containing either glucose or fructose to determine the extent of variation in extracellular protein production that co ... | 1986 | 2945902 |
| monoclonal antibodies to the extracellular glucosyltransferases from streptococcus sobrinus 6715. | murine monoclonal antibodies (mabs) were raised against the glucosyltransferases (gtfs) of streptococcus sobrinus 6715. the antibody panels included mabs raised against the primer-independent, soluble product enzyme (gtf-si) which did not cross-react with other gtfs, as well as mabs raised against the primer-dependent, soluble product enzyme (gtf-sd) which recognized both gtf-si and gtf-sd, thus indicating that these catalytically distinct enzymes share epitopes. mabs raised against gtf-i recogn ... | 1987 | 2956196 |
| characterization of glucosyltransferase expressed from a streptococcus sobrinus gene cloned in escherichia coli. | the gene encoding a glucosyltransferase which synthesized water-insoluble glucan, gtfi, previously cloned from streptococcus sobrinus strain mfe28 (mutans serotype h) into a bacteriophage lambda vector, was subcloned into the plasmid pbr322. the recombinant plasmid was stable in escherichia coli and gtfi was efficiently expressed. the gtf-i expressed in e. coli was compared to the corresponding enzymes in s. sobrinus strains mfe28 (serotype h), b13 (serotype d) and 6715 (serotype g) and shown to ... | 1987 | 2958599 |
| oral immunization of humans with streptococcus sobrinus glucosyltransferase. | the effect of oral administration of glucosyltransferase (gtf) from streptococcus sobrinus 6715 on levels of immunoglobulin a (iga) antibody to gtf in parotid saliva and on the number of indigenous streptococcus mutans in the whole saliva was studied in young adult males. gtf combined with aluminum phosphate (ap) was administered in capsules to 14 subjects, while sodium phosphate buffer combined with ap was administered in the same way to 11 control subjects. thirteen administrations were given ... | 1987 | 2959623 |
| isolation of a glucan-binding domain of glucosyltransferase (1,6-alpha-glucan synthase) from streptococcus sobrinus. | a glucan-binding domain of 1,6-alpha-glucan synthase (dextransucrase) (gtf-s) was isolated from a trypsin digest of the streptococcus sobrinus enzyme. the large 60.5-kilodalton peptide had an affinity for dextran comparable to that of the native enzyme, but had no glucan synthesis activity. the domain was produced in high yield compared with other large cleavage products, which allowed easy purification by size exclusion high-pressure liquid chromatography and affinity chromatography. two other ... | 1988 | 2964413 |
| homology of glucosyltransferase gene and protein sequences from streptococcus sobrinus and streptococcus mutans. | the sequences of glucosyltransferase genes from streptococcus sobrinus (gtfi) and streptococcus mutans (gftb) were compared and show a high degree of homology. there is a 57.7% homology of nucleotides in the genes and a 56.7% homology of amino acids in the deduced protein sequences. the g + c content for the protein-coding region is 43.6% for s. sobrinus and 41.2% for s. mutans. internal repeating sequences present in both proteins exhibit some difference in sequence pattern. | 1988 | 2971696 |
| the caries inhibitory effects of gos-sugar in vitro and in rat experiments. | the caries inhibitory activity of gos-sugar (panose- and maltose-rich sugar mixture) was examined and compared with that of sucrose, maltose, or glucose in in vitro and in vivo experiments. streptococcus mutans mt8148r (serotype c) and streptococcus sobrinus 6715 (g) did ferment gos-sugar and produce acid in a similar way as with maltose and glucose. however, gos-sugar could not be a substrate for the glucosyltransferases (gtases) of these mutans streptococci to synthesize the water-insoluble gl ... | 1988 | 2975747 |
| reduction of the adherence of streptococcus sobrinus insoluble alpha-d-glucan by endo-(1----3)-alpha-d-glucanase. | insoluble alpha-d-glucan, previously formed on a glass surface from sucrose by the action of cell-free d-glucosyltransferases of streptococcus sobrinus omz176, was significantly removed by a purified preparation of endo-(1----3)-alpha-d-glucanase (mutanase) from a strain of pseudomonas sp. almost complete dissociation of adherent glucan occurred at the highest enzyme concentration (40 mu/ml) tested. synthesis and de novo adherence on glass of the glucan was markedly inhibited by the presence of ... | 1988 | 2977294 |
| effects of bicarbonate-based dental powder, fluoride, and saccharin on dental caries and on streptococcus sobrinus recoveries in rats. | the effects of nahco3-based dental powder containing naf and sodium saccharin on dental caries and streptococcus sobrinus recoveries in rats were studied. weanling specific-pathogen-free osborne-mendel (spfom) rats were inoculated with s. sobrinus 6715-13wt. one of six infected groups was topically treated with either demineralized water (dw), a dental powder suspended such that there was 1 part solid per 2 parts dw, 0.073% naf, 0.5% na-saccharin (nas), or a combination of naf and nas at the sam ... | 1987 | 3038976 |
| nucleotide sequence of a glucosyltransferase gene from streptococcus sobrinus mfe28. | the complete nucleotide sequence was determined for the streptococcus sobrinus mfe28 gtfi gene, which encodes a glucosyltransferase that produces an insoluble glucan product. a single open reading frame encodes a mature glucosyltransferase protein of 1,559 amino acids (mr, 172,983) and a signal peptide of 38 amino acids. in the c-terminal one-third of the protein there are six repeating units containing 35 amino acids of partial homology and two repeating units containing 48 amino acids of compl ... | 1987 | 3040686 |
| molecular cloning and expression of a streptococcus mutans major surface protein antigen, p1 (i/ii), in escherichia coli. | antigen p1, also called i/ii, is one of the most abundant cell wall proteins of the mutans streptococci. it has been suggested that p1 may be involved in cell adherence to tooth surfaces and in sucrose-induced cell aggregation. as a first step toward fully understanding its biological functions, the p1 gene, which has been designated spap1, from streptococcus mutans ng5 (serotype c) has been cloned into escherichia coli jm109 by a shotgun procedure with puc18 as the vector. the recombinant strai ... | 1988 | 3135272 |
| chemical and structural studies of serotype polysaccharide antigens of streptococcus sobrinus 6715. | the g antigen of streptococcus sobrinus 6715 was previously shown to consist of polysaccharides of various molecular weights. in this study, two such polysaccharides, lii and liii, were purified by gel filtration and affinity chromatography procedures. by a double immunodiffusion analysis, fraction lii was found to contain a region in the serotype-specific g site not present in the serospecific g site of fraction liii. this region was designated x. in addition to the serotype-specific g site, th ... | 1988 | 3139566 |
| suppression of streptococcus sobrinus 6715 (g) in plaques by streptococcus mutans 32k (c). | the dental plaque of 96 healthy donors was screened for the production of such antibacterial substances as mutalipocin and bacteriocin and 192 strains of mutans streptococci isolated: 28 produced mutalipocin and 22 produced bacteriocin. mutalipocin produced by these 28 s. mutans strains possessed similar biochemical and biological characteristics of well-characterized mutalipocin-producing strain s. mutans 32k (serotype c). when equal amounts of s. mutans 32k and s. sobrinus 6715 (g) were cultur ... | 1988 | 3150434 |
| synergistic antibacterial effects of copper and hexetidine against streptococcus sobrinus and streptococcus sanguis. | the aim of this study was to determine whether a combination of copper and hexetidine had a synergistic antibacterial effect against streptococcus sobrinus omz 176 and s. sanguis 10556. concentration ranges of the test agents alone and in combination were prepared by serial dilutions in microtiter trays with brain-heart infusion (bhi) broth as the bacterial growth medium. after incubation at 37 degrees c for 24 h, the minimum inhibitory concentration (mic), corresponding to the lowest concentrat ... | 1988 | 3165588 |
| in-vitro adherence of oral streptococci in the presence of sucrose and its relationship to cariogenicity in the rat. | streptococcus sanguis i gave a significantly greater percentage coverage (cell-pellicle attachment) of saliva-coated glass in the presence of sucrose than did strep. sanguis ii (p less than 0.025), and both these gave greater percentage coverages than the other species tested, between which no significant differences were noted. there was a large number of significant differences in clump size (cell-cell attachment) between species-pairs. among the mutans streptococci, there were significant dif ... | 1988 | 3166609 |
| combined effect of xylitol, naf and zncl2 on growth and metabolism of streptococcus sobrinus omz 176. | xylitol, naf and zncl2 in combination inhibited the growth of s. sobrinus omz 176 when added to brain heart infusion broth. thin-layer chromatography, followed by autoradiography of cell extracts, was used to study the inhibiting mechanism. glucose uptake was reduced, the glycolysis inhibited at the glucose 6-phosphate- and fructose- 1.6-diphosphate level and the accumulation of xylitol metabolites was increased. these effects in combination probably accounted for the inhibition of growth. | 1988 | 3166805 |
| influence of desalivation in rats on incidence of caries in intact cagemates. | the evidence that dental caries is an infectious and transmitted disease in rodents is unequivocal. however, the factors controlling the transmission of micro-organisms from one animal to another have not been extensively explored. results from previous studies in our laboratory showed that desalivated animals became infected by streptococcus sobrinus in a shorter period of time than did intact animals. furthermore, an additional study in our laboratory showed that animals with intact salivary f ... | 1988 | 3170887 |
| the effects of different levels of dietary sucrose on root caries subsequent to gingivectomy in conventional rats infected with actinomyces viscosus m-100. | three groups of weanling, sprague-dawley-derived rats were inoculated with actinomyces viscosus m-100 and fed powdered diet containing either 67%, 5%, or 0% confectioner's sugar. two further groups were fed diet containing 5% confectioner's sugar and inoculated with streptococcus sobrinus 6715 or s. sobrinus 6715 plus a. viscosus m-100. the most coronal 1 mm of gingiva was removed from maxillary and mandibular right molar quadrants (gingivectomy), and the animals re-inoculated following gingivec ... | 1988 | 3170889 |
| the in vitro dental plaque inhibitory properties of a series of n-[1-alkyl-4(1h)-pyridinylidene]alkylamines. | a series of novel n-[1-alkyl-4(1h)-pyridinylidene]alkylamine hydrohalides has been prepared and evaluated as inhibitors of dental plaque formation, in vitro. several members of the series exhibited potency ca. 9-fold greater than that of chlorhexidine vs streptococcus sobrinus 6715-13. the di-n-octyl analogue, 11 (pirtenidine), was found to be highly efficacious against several other oral plaque-forming microorganisms and is presently undergoing preclinical evaluation. | 1988 | 3172139 |
| utilization of a continuous streptococcal surface to measure interbacterial adherence in vitro and in vivo. | cell-to-cell interactions are essential for the formation of dental plaque. a continuous layer of streptococcus sanguis sa-1 cells fixed to a solid surface has been used to evaluate interactions among this bacterium, haemophilus parainfluenzae, and streptococcus sobrinus. s. sanguis cells were attached to a falcon 3001 tissue culture plates or bovine enamel chips, coated with a biological adhesive. scanning electron microscopy of the chips showed the streptococci as a contiguous surface. radiola ... | 1988 | 3198842 |
| colonization of rat molar teeth by mutans streptococci with different salivary agglutination characteristics. | the oral implantation of salivary agglutination-positive and -negative mutans streptococci was studied using streptomycin resistant (strr) organisms. strr streptococcus mutans strains ingbritt and nctc 10449 are agglutinated by rat saliva and the strr strains streptococcus sobrinus 6715-13 and strep. mutans gs5 are not. four groups of sprague-dawley rats were inoculated orally with each organism (one per group) and fed a sucrose diet. a further two groups of animals were similarly inoculated wit ... | 1988 | 3252771 |
| prevalence of mutans streptococci and lactobacilli in elderly swedish individuals. | the number of streptococcus mutans, streptococcus sobrinus, and lactobacilli in stimulated whole saliva was examined in 149 elderly persons. more than 10(6) cfu/ml of mutans streptococci were found in 57% of the subjects and 85% had more than 10(5) lactobacilli per ml of saliva. there was no difference in the prevalence of mutans streptococci between edentulous and dentate subjects whereas significantly more lactobacilli were detected in denture wearers. s. mutans was most frequent and was found ... | 1988 | 3277259 |
| avirulent salmonella typhimurium delta cya delta crp oral vaccine strains expressing a streptococcal colonization and virulence antigen. | salmonella typhimurium sr-11 strains lacking adenylate cyclase and the cyclic amp receptor protein (crp) due to deletion (delta) mutations in the cya and crp genes, respectively, are avirulent for mice and induce high level protective immunity against subsequent challenge with wild-type virulent s. typhimurium sr-11 cells. the avirulence of these delta cya delta crp mutants has been enhanced by elimination of the 100 kb virulence plasmid pstsr100 without impairing immunogenicity. the present rep ... | 1988 | 3291452 |
| effects of chlorhexidine gluconate in drinking water on dental caries and oral microorganisms in the syrian hamster. | hamsters infected with streptococcus sobrinus (formerly streptococcus mutans) strain 6715-1119 were given high-sucrose diet 2000. after 5 days, they were presented with 0.05 or 0.1 per cent w/v chlorhexidine gluconate (chx) continuously in their drinking water, which was well-tolerated. compared to infected but untreated controls, their caries scores were less by 84 and 97 per cent respectively after 42 days. coronal dental plaque deposits were reduced in a dose-related fashion. culture plates o ... | 1986 | 3307713 |
| identification of streptococcus sobrinus with monoclonal antibodies. | identification of streptococcus sobrinus is often difficult to perform because of the great resemblance of the organism to other oral streptococcal species. therefore, monoclonal antibodies were prepared which were shown to be highly specific for s. sobrinus. cross-reactivity with other oral microorganisms has not been observed in an enzyme-linked immunosorbent assay and an immunofluorescence assay. these monoclonal antibodies belonged to the subclass immunoglobulin g2b. to be certain that the s ... | 1987 | 3323224 |
| effect of nutritional constraints on the biosynthesis of the components of the phosphoenolpyruvate: sugar phosphotransferase system in a fresh isolate of streptococcus mutans. | a procedure for the purification of enzyme i (ei) and the protein hpr, the general components of the phosphoenolpyruvate:sugar phosphotransferase system, from streptococcus mutans serotype c is presented. the method was also applied successfully to the purification of ei and hpr from streptococcus salivarius, streptococcus sobrinus, and streptococcus sanguis. using specific antibodies obtained against the proteins purified from s. mutans dr0001, we determined quantitatively by rocket electrophor ... | 1988 | 3338847 |
| c31g, a new agent for oral use with potent antimicrobial and antiadherence properties. | c31g, an equimolar mixture of alkyl dimethyl glycine and alkyl dimethyl amine oxide, was evaluated for antimicrobial and antiadherence properties. the efficacy of c31g, its two components, and several commercial mouth rinses was determined in assays measuring inhibition of glycolysis, inhibition of bacterial adherence, and mics. inhibition of glycolysis was determined by using a saliva sediment model, with glycolytic activity expressed as the change in ph relative to that of a control. adherence ... | 1988 | 3364952 |
| specificity of the glucan-binding lectin of streptococcus cricetus. | the specificity of the glucan-binding lectin (gbl) of streptococcus cricetus aht was determined. examination of the kinetics of aggregation of cell suspensions with glucans containing various percentages of alpha-1,6, alpha-1,4, alpha-1,3, and alpha-1,2 anomeric linkages revealed that only glucans with at least 80% alpha-1,6 linkages promoted strong aggregation. moreover, only linear glucans with molecular weights greater than 5 x 10(5) were capable of causing rapid aggregation of the bacteria. ... | 1988 | 3397177 |
| in vitro studies of growth and competition between s. salivarius tove-r and mutans streptococci. | streptococcus salivarius tove-r has previously been reported as a successful competitor with streptococcus mutans 10449s and streptococcus sobrinus 6715-13 wt on the teeth of rats. we studied, in vitro, some possible bases for this competition, including hydrogen peroxide or catalase production, bacteriocin or enocin production, and growth rates. growth rates were measured spectrophotometrically in both complex and defined media. we studied conditions of aerobic and anaerobic incubation; glucose ... | 1986 | 3461031 |
| root surface caries subsequent to gingivectomy in rats inoculated with streptococcus sobrinus (mutans) and actinomyces viscosus. | we studied the effect of oral infection with cariogenic micro-organisms on alveolar bone loss and root surface caries subsequent to gingivectomy in rats. thirty-six rats were fed diet mit 200 (67% sucrose); one-half, the controls, had antibiotics added to the diet. at ages 18, 19, and 21 days, the experimental group was orally infected with streptomycin-resistant streptococcus sobrinus (mutans) 6715 and actinomyces viscosus m-100. at age 30 days, all animals were subjected to a gingivectomy on m ... | 1987 | 3476557 |
| effects of naf and snf2 on growth, acid and glucan production of several oral streptococci. | the effects of low concentrations of these fluorides on streptococcus sobrinus, streptococcus mutans, streptococcus salivarius, streptococcus mitior and streptococcus sanguis were investigated. without fluoride, mutans streptococci (strep. mutans and strep. sobrinus) produced more acid than the other strains; both fluorides reduced acid production in all strains. naf had little effect on growth; snf2 decreased growth in all strains, most evidently in the strep. mutans. in all growth conditions, ... | 1987 | 3482350 |
| haptenated streptococcal antigens elicit either t cell-dependent type 1 or t cell-independent type 2 immune responses. | antigens of streptococcus mutans 6715 (alternatively designated serotype g streptococcus sobrinus), including whole cells (wc g), cell walls (cw g), peptidoglycan (pg g) and serotype carbohydrate (ml g) were coupled with trinitrophenyl (tnp), and the nature of the immune response to each immunogen was determined in normal and x-linked immunodeficient (xid) murine spleen cell cultures. responses to tnp-wc g, -cw g and -pg g and to the classical type 1 antigen tnp-brucella abortus occurred in both ... | 1987 | 3496265 |
| protection of gnotobiotic rats against dental caries by passive immunization with bovine milk antibodies to streptococcus mutans. | a multivalent vaccine consisting of whole cell antigens of seven strains, representing four serotypes (b, c, d and g), of mutans streptococci was used to hyperimmunize a group of cows. serum samples from these animals contained immunoglobulin g1 (igg1) antibody activity to seven serotypes (a to g) of mutans streptococci. whey obtained from the animal with the highest serum antibody activity, which also contained high levels of igg1 antibody, was used in passive caries immunity studies. gnotobiot ... | 1987 | 3498689 |
| purification and partial characterization of the multicomponent dextranase complex of streptococcus sobrinus and cloning of the dextranase gene. | the presence of proteases in culture supernatant fluids and on the cell surface of streptococcus sobrinus and the aggregation of multicomponent enzyme complexes make the isolation and characterization of cell surface proteins difficult. we report a simple purification procedure for dextranase and the cloning of the dextranase structural gene. s. sobrinus culture supernatant fluids were precipitated with 70% ammonium sulfate, and the precipitate was dialyzed against sodium acetate buffer and load ... | 1987 | 3546141 |
| characterization of a rat salivary sialoglycoprotein complex which agglutinates streptococcus mutans. | rat saliva agglutinated streptococcus mutans ingbritt and nctc 10449 and streptococcus sanguis nctc 7864 but not s. mutans nctc 10921, gs 5, or lm 7, streptococcus sobrinus 6715-13 or omz 65, or streptococcus cricetus hs 6, as measured turbidometrically. the specificity of agglutination by rat saliva was the same as that by human saliva. agglutination was associated with a mucin complex (rat salivary agglutinin complex [rs-a]) of sulfated sialoglycoproteins, with a trace of associated lipid and ... | 1987 | 3570462 |
| effect of growth conditions on levels of components of the phosphoenolpyruvate:sugar phosphotransferase system in streptococcus mutans and streptococcus sobrinus grown in continuous culture. | the membrane-bound, sugar-specific enzyme ii (eii) component of the phosphoenolpyruvate:sugar phosphotransferase system (pts) in streptococcus mutans ingbritt is repressed by growth on glucose under various conditions in continuous culture. compared with optimal pts conditions (i.e., glucose limitation, dilution rate [d] of 0.1 h-1, and ph 7.0), eii activity for glucose (eiiglc) and mannose (eiiman) in cells grown at a d of 0.4 h-1 and ph 5.5 with the same glucose concentration was reduced 24- t ... | 1987 | 3680174 |
| characterization of immune response to oral administration of streptococcus sobrinus ribosomal preparations in liposomes. | gnotobiotic rats gastrically intubated with a total of 12.5 micrograms of streptococcus sobrinus ribosomal protein incorporated into cholesterol-based liposomes had significantly (p less than or equal to 0.01) fewer carious lesions on their molar surfaces than did nonimmunized infected controls after challenge with a virulent organism. the immunized animals had significantly (p less than or equal to 0.01) lower numbers of molar-adherent s. sobrinus cells and higher levels of salivary immunoglobu ... | 1986 | 3781625 |
| competitive displacement of mutans streptococci and inhibition of tooth decay by streptococcus salivarius tove-r. | the ability of streptococcus salivarius tove-r to displace virulent representatives of the most prevalent human mutans streptococci from the teeth of rats, and thereby to inhibit caries, was studied. streptococcus mutans 10449s- or streptococcus sobrinus 6715-13wt-infected specific-pathogen-free rats consuming a high-sucrose diet were inoculated by tove-r. the infectants were differentially recovered from swabs of the teeth over the time course of infection and from sonically treated material of ... | 1985 | 3980093 |
| structural studies of the rhamnose-glucose polysaccharide antigen from streptococcus sobrinus b13 and 6715-t2. | the rhamnose-glucose polysaccharide antigens from the cell walls of streptococcus sobrinus b13 and 6715-t2 (formerly streptococcus mutans serotypes d and g, respectively) were structurally examined by using gas chromatography-mass spectroscopy. these data confirmed earlier chemical and serological studies suggesting that these polysaccharides had identical structures. the polysaccharides appeared to have a backbone of alternating 1,2- and 1,3-linked rhamnose units. branching occurred at 1,2,3-li ... | 1985 | 4055034 |
| dextran-induced aggregation in a mutant of streptococcus sobrinus 6715-13. | a mutant of wild-type streptococcus sobrinus 6715-13 has been isolated which resists aggregation by exogenous dextran. this variant is able to form adherent plaque deposits in vitro when cultured in the presence of sucrose and has dextranase activity. in these respects it is the complement of previously described isolates which are plaque formation defective but aggregation normal. measurements of the incorporation of glucose from glucosyl-labeled sucrose into glucan by cell-associated glucosylt ... | 1983 | 6190754 |
| immunoperoxidase labeling of streptococcus mutans for scanning electron microscopy. | the application of an immunoperoxidase method to labeling of streptococcus mutans subsp. sobrinus for scanning electron microscopy is described. bacteria from four separate cultures were fixed in 2.5% buffered glutaraldehyde solution. the specimens were treated in accordance with an indirect immunolabeling procedure using a rabbit anti-s. mutans subsp. sobrinus gamma-globulin. labeled bacteria were equipped with a layer of small, distinct globules of horse-radish peroxidase reaction product. bac ... | 1983 | 6349231 |
| penicillin-induced lysis of streptococcus mutans. | treatment of streptococcus mutans gs-5 cells with concentrations of penicillin g within a relatively narrow range resulted in substantial lysis. this penicillin-induced lysis was dependent upon cell density and ph of the lysis medium. other oral streptococci (streptococcus sobrinus, streptococcus rattus, and streptococcus cricetus) also demonstrated substantial levels of penicillin-induced lysis under appropriate conditions. lesser degrees of lysis were seen in a related organism, streptococcus ... | 1984 | 6500698 |
| prevalence, distribution of serotypes, and cariogenic potential in hamsters of mutans streptococci from elderly individuals. | the prevalence of mutans streptococci (streptococcus mutans, streptococcus cricetus, streptococcus sobrinus, and streptococcus rattus) was determined in the salivas of 169 elderly individuals ranging in age from 60 to 87 years. approximately 40% of these individuals were edentulous and wore full upper and lower dentures. with the exception of a higher proportion of saliva counts below 1,000 cfu/ml in the full-denture wearers, the prevalence and the serotype and species distributions of the mutan ... | 1983 | 6874072 |
| colonization and cariogenicity of streptococcus ferus in rats. | streptococcus ferus, which is indigenous to wild rats, is a member of the mutans group of streptococci. we tested its ability to colonize and to cause caries in laboratory rats by comparing two strains of s. ferus with the very cariogenic streptococcus sobrinus strain 6715. groups of rats were fed either finely ground mouse chow or a 56% sucrose diet, or they were switched from chow to the sucrose diet. all three strains colonized the mouths of rats regardless of diet. however, the infectants re ... | 1981 | 7216496 |
| in vitro modulation of oral bacterial adhesion to saliva-coated hydroxyapatite beads by milk casein derivatives. | bovine caseinate, derivatives of its glycosylated moiety [caseinoglycomacropeptide (cgp)], and caseinophosphopeptides were evaluated as inhibitors of adhesion of oral bacteria to saliva-coated hydroxyapatite beads (s-ha). all milk casein-derived components behaved as potent inhibitors of streptococcus sanguis omz 9 and streptococcus sobrinus omz 176 adhesion to s-ha, whereas neither bovine serum albumin nor polyethyleneglycol were able to interfere with the adhesion of these strains. by contrast ... | 1994 | 7478758 |