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development and behavior: older children and adolescents. 20061574349
neonatal nurse practitioners--a view from perfidious albion? 20061586193
[b-cell chronic lymphocytic leukemia complicated by autoimmune thrombocytopenic purpura].a 74-year-old japanese male was admitted because of anemia. hepatosplenomegaly, lymphoadenopathy, and purpura were not found. the laboratory data on admission revealed that the white-cell count was 9,400/microliters, the hemoglobin 11.1 g/dl, and the platelet count 17,000/microliters. platelet-associated igg was 794.2 ng/10(7) cells. the patient was diagnosed as having autoimmune thrombocytopenic purpura (atp) at this time. he was treated with prednisolone, but his thrombocytopenia not improve. ...20061635169
enzymes associated with metabolism of xylose and other pentoses by prevotella (bacteroides) ruminicola strains, selenomonas ruminantium d, and fibrobacter succinogenes s85.prevotella (bacteroides) ruminicola strains b(1)4 and s23 and selenomonas ruminantium strain d used xylose as the sole source of carbohydrate for growth, whereas fibrobacter succinogenes was unable to metabolize xylose. prevotella ruminicola strain b(1)4 exhibited transport activity for xylose. in contrast, f. succinogenes lacked typical xylose uptake activity but did exhibit low binding potential for the sugar. prevotella ruminicola strains b(1)4 and s23 as well as s. ruminantium d showed low x ...19921643581
utilization of nucleic acids by selenomonas ruminantium and other ruminal bacteria.species of ruminal bacteria were screened for the ability to grow in media containing rna or dna as the energy source. bacteroides ruminicola d31d and selenomonas ruminantium hd4, ga192, and d effectively used rna for growth, but not dna. b. ruminicola d31d was able grow on nucleosides but not on bases or ribose. the s. ruminantium strains were able to grow when provided with either nucleosides or ribose but not bases. strains of s. ruminantium, but not b. ruminicola d31d, were also able to use ...19901707252
effect of a saccharomyces cerevisiae culture on lactate utilization by the ruminal bacterium selenomonas ruminantium.the objective of this study was to examine the effects of a saccharomyces cerevisiae culture (yea-sacc) on lactate utilization by the predominant ruminal bacterium selenomonas ruminantium. lactate uptake was stimulated by yea-sacc concentrations between 2.5 and 10 g/liter, and the 5-g/liter level increased uptake 3.8-fold. when yea-sacc concentrations were increased above the 5-g/liter level lactate uptake was decreased, but 10 g/liter still stimulated uptake more than threefold. a filter-steril ...19911752834
intracellular ph of acid-tolerant ruminal bacteria.acid-tolerant ruminal bacteria (bacteroides ruminicola b1(4), selenomonas ruminantium hd4, streptococcus bovis jb1, megasphaera elsdenii b159, and strain f) allowed their intracellular ph to decline as a function of extracellular ph and did not generate a large ph gradient across the cell membrane until the extracellular ph was low (less than 5.2). this decline in intracellular ph prevented an accumulation of volatile fatty acid anions inside the cells.19911781695
esorubicin in advanced endometrial cancer: an ineffective and potentially toxic therapy. a southwest oncology group study.the southwest oncology group conducted a phase ii study of esorubicin treatment in patients with advanced endometrial cancer who had received no prior chemotherapy. twenty of 31 patients were fully evaluable for response and toxicity. there were no clinical responses to treatment and 60% (12/20) of the patients developed severe or life threatening leukopenia on therapy. one evaluable patient was removed from study after a cumulative dose of 150 mg/m2 due to a reduction in left ventricular ejecti ...20071874602
adaptive changes in the developing brain during intrauterine stress.maturation of neurological performance in moderately to severely growth-retarded newborn infants (sga) can be accelerated by 3 to 4 weeks or more when compared to the development of appropriately grown infants (aga) of the same gestation. this is particularly the case in multiple pregnancies or pregnancies characterized by maternal hypertension. this clinical finding has been confirmed by neurophysiological studies on the maturation of brainstem auditory evoked responses (baers). the possible me ...20061892222
succinate transport by a ruminal selenomonad and its regulation by carbohydrate availability and osmotic strength.washed cells of strain h18, a newly isolated ruminal selenomonad, decarboxylated succinate 25-fold faster than selenomonas ruminantium hd4 (130 versus 5 nmol min-1 mg of protein-1, respectively). batch cultures of strain h18 which were fermenting glucose did not utilize succinate, and glucose-limited continuous cultures were only able to decarboxylate significant amounts of succinate at slow (less than 0.1 h-1) dilution rates. strain h18 grew more slowly on lactate than glucose (0.2 versus 0.4 h ...19912036012
[problem of clinico-roentgenological diagnosis of acute pneumonia in infants in their first few months of life].studies into the problems of diagnosing pneumonia in children of the first months of life carried out for many years have shown that pneumonia may be referred to the diseases with indefinite diagnostic criteria. in view of this fact the x-raying of the chest should be regarded as a method of varifying the diagnosis of pneumonia. the roentgenographic findings should be estimated bearing in mind the presence or lack of a syndrome such as bronchial obstruction and the epidemiological anamnesis data ...20072075041
site-specific rate constants for iron removal from diferric transferrin by nitrilotris(methylenephosphonic acid) and pyrophosphate.the kinetics of iron removal from human serum diferric transferrin by nitrilotris(methylenephosphonic acid) (ntp) and pyrophosphate (ppi) have been studied in 0.1 m, ph 7.4, n-(2-hydroxyethyl)piperazine-n'-2-ethanesulfonate buffer at 25 degrees c using urea/polyacrylamide gel electrophoresis. the four microscopic rate constants required for a complete description of iron removal from the two transferrin metal-binding sites have been measured at 100 mm concentrations of ntp and ppi. there is very ...19902168158
[the electromyographic analysis of trembling hyperkinesis in wilson-konovalov's hepatocerebral dystrophy].global electromyography was applied in the examination of 20 patients with wilson-konovalov's hepatocerebral dystrophy before and after stereotaxic operations. the origin of volley muscular activity is explained on the basis of segmental reflexes known in neurophysiology: indirect relief of alpha-motoneurons from the primary terminals of the spindle, autogenous, recurrent and reciprocal inhibition of alpha-motoneurons.20072168651
localization of bacterial antigens in calves inoculated orally with ruminal bacteroides succinogenes and selenomonas ruminantium.localization of bacteria and bacterial materials was investigated in calves inoculated orally with live organisms of both bacteroides succinogenes and selenomonas ruminantium by a immunohistological method using rabbit antiserum against the outer membrane of those organisms and by a scanning electron microscope. the intact organisms of both inoculated bacterial species were detected on the rumen wall and in the lamina propria of the forestomach, and s. ruminantium also in the lymph nodes associa ...19902202844
potential anti-hiv active pyranoid analogs of azt. 19902206706
cytotoxic activity against hiv-infected monocytes by recombinant interleukin 2-activated natural killer cells.natural killer (nk) cells have long been known to aid in the control of viral infections by killing virus-infected cells, including those infected with human immunodeficiency virus (hiv). among the possible nk-susceptible target cells in an infected individual, the monocyte/macrophages are of special significance since they may serve as both a reservoir of hiv and aid in dissemination of the virus throughout the body. a new technique for the enrichment and cultivation of large numbers of recombi ...19902223237
cytotoxic activity against hiv-infected monocytes by recombinant interleukin 2-activated natural killer cells.natural killer (nk) cells have long been known to aid in the control of viral infections by killing virus-infected cells, including those infected with human immunodeficiency virus (hiv). among the possible nk-susceptible target cells in an infected individual, the monocyte/macrophages are of special significance since they may serve as both a reservoir of hiv and aid in dissemination of the virus throughout the body. a new technique for the enrichment and cultivation of large numbers of recombi ...19902223237
folding and stability of trp aporepressor from escherichia coli.equilibrium and kinetic studies of the urea-induced unfolding of trp aporepressor from escherichia coli were performed to probe the folding mechanism of this intertwined, dimeric protein. the equilibrium unfolding transitions at ph 7.6 and 25 degrees c monitored by difference absorbance, fluorescence, and circular dichroism spectroscopy are coincident within experimental error. all three transitions are well described by a two-state model involving the native dimer and the unfolded monomer; the ...19902223756
folding and stability of trp aporepressor from escherichia coli.equilibrium and kinetic studies of the urea-induced unfolding of trp aporepressor from escherichia coli were performed to probe the folding mechanism of this intertwined, dimeric protein. the equilibrium unfolding transitions at ph 7.6 and 25 degrees c monitored by difference absorbance, fluorescence, and circular dichroism spectroscopy are coincident within experimental error. all three transitions are well described by a two-state model involving the native dimer and the unfolded monomer; the ...19902223756
[simplification test in the choice of impression techniques for removable partial dentures]. 20082258231
generation of a membrane potential by sodium-dependent succinate efflux in selenomonas ruminantium.when selenomonas ruminantium hd4 was grown in a chemostat, maximal succinate production and the highest molar growth yield values were both observed at a dilution rate of roughly 0.2 h-1. to determine the possible relationship between succinate efflux and high molar growth yields, the generation of a membrane potential by succinate efflux was studied in whole cells and vesicles (inside-out and right-side-out) prepared from s. ruminantium. washed whole cells took up succinate in the absence of an ...19902307654
detection of bovine antibodies to the outer membrane of ruminal bacteroides succinogenes by enzyme-linked immunosorbent assay (elisa).the enzyme-linked immunosorbent assay (elisa) was used to detect bovine serum antibodies directed to the outer membrane antigen of a ruminal bacteria, bacteroides succinogenes. the outer membrane antigen of b. succinogenes was highly reactive against homologous antiserum, compared with rabbit sera raised against b. ruminicola subsp. ruminicola, b. ruminicola subsp. brevis and selenomonas ruminantium. the titers of sera from colostrum-deprived calves were negligible level, while those of sera fro ...19902313950
xylose uptake by the ruminal bacterium selenomonas ruminantium.selenomonas ruminantium hd4 does not use the phosphoenolpyruvate phosphotransferase system to transport xylose (s. a. martin and j. b. russell, j. gen. microbiol. 134:819-827, 1988). xylose uptake by whole cells of s. ruminantium hd4 was inducible. uptake was unaffected by monensin or lasalocid, while oxygen, o-phenanthroline, and hgcl2 were potent inhibitors. menadione, antimycin a, and kcn had little effect on uptake, and acriflavine inhibited uptake by 23%. sodium fluoride decreased xylose up ...19902383009
monoclonal antibodies against the ruminal bacterium selenomonas ruminantium.monoclonal antibodies were raised against whole cells of two different strains of selenomonas ruminantium and tested for specificity and sensitivity in immunofluorescence and enzyme-linked immunosorbent assay procedures. species-specific and strain-specific antibodies were identified, and reactive antigens were demonstrated in solubilized cell wall extracts of s. ruminantium. a monoclonal antibody-based solid-phase immunoassay was established to quantify s. ruminantium in cultures or samples fro ...19902389936
white noise analysis of temporal properties in simple receptive fields of cat cortex.we studied the linear and nonlinear temporal response properties of simple cells in cat visual cortex by presenting at single positions in the receptive field an optimally oriented bar stimulus whose luminance was modulated in a random, binary fashion. by crosscorrelating a cell's response with the input it was possible to obtain the zeroth-, first-, and second-order wiener kernels at each rf location. simple cells showed pronounced nonlinear temporal properties as revealed by the presence of pr ...19902390534
distribution of immunoglobulin-containing cells in calves inoculated orally with ruminal bacteroides succinogenes and selenomonas ruminantium.distribution of immunoglobulin(ig)-containing cells was investigated in calves inoculated orally with live organisms of both bacteroides succinogenes and selenomonas ruminantium. pathological changes and many ig-containing cells were observed in calves which inoculated three times at 2, 3 and 26 days of age. follicular germinal center was increased in number and size of the lymph nodes associated with the forestomach, suggesting activation of lymph apparatus. in the associated lymph nodes, igg-c ...19902391773
interaction of carbaryl and n-nitrosocarbaryl with microsomal monooxygenase activities.the in vitro interactions of carbaryl and carcinogenic n-nitrosocarbaryl with rat liver microsomal monooxygenase activities are compared. the inhibitory effect of the nitroso-compound is demonstrated to be non-competitive on aminopyrine n-demethylase, p-nitroanisole o-demethylase and aniline hydroxylase. the nature of the inhibition induced by the parent amide is found to be competitive on aminopyrine n-demethylase and p-nitroanisole o-demethylase. correspondingly, in vitro studies of the metabo ...19892493170
characterization of a plasmid from the ruminal bacterium selenomonas ruminantium.a 4.8-kilobase-pair plasmid was isolated from the ruminal bacterium selenomonas ruminantium hd4 by using a sodium carbonate-edta washing buffer to improve cell lysis (r.g. dean, s.a. martin, and c. carver, lett. appl. microbiol. 8:45-48, 1989). this plasmid, designated psr1, appears to be quite stable. no evidence of plasmid dna was detected in s. ruminantium d or ga192. all three strains were tested for antibiotic resistance, and all were kanamycin resistant (mic, 25 to 50 micrograms/ml). only ...19892619301
[arthrographic study on temporomandibular joint sounds. the relationship between morphology of articular disk and style, timing of temporomandibular joint sounds]. 20082637367
newer agents for coronary thrombolysis. perspectives from clinical studies.myocardial infarction (mi) is a major cause of morbidity and mortality in western countries and the formation of intracoronary thrombi is recognized as a critical determinant of this ischaemic event. since streptokinase and urokinase cause in vitro lysis of clots, it was though that these drugs were also effective in vivo in dissolving coronary thrombi. clinical studies supported this concept. however, the beneficial effects of these drugs were, to some extent, offset by their inherent adverse r ...20092664750
the pectinolytic enzyme of selenomonas ruminantium.a cell-bound pectinolytic enzyme was isolated from cells of selenomonas ruminantium and purified about 360-fold. the optimum ph and temperature for enzyme activity was 7.0 and 40 degrees c. the enzyme degraded polymeric substrates by hydrolysis of digalacturonic acid units from the non-reducing end; the best substrate was nonagalacturonic acid. unsaturated trigalacturonate was also degraded, but 30% slower than the saturated analogue. the enzyme was classified as a poly (1,4-alpha-d-galactosidur ...19892708171
computereyes video image digitizer. 20092709974
[the primary care physician and the problems of aid to patients with acquired immunodeficiency syndrome]. 20092716378
the characterization and ultrastructure of two new strains of butyrivibrio.strains b-385-1 and 2-33 are numerically important components rumen bacterial populations , but they have remained (taxonomically) undefined. in spite of some resemblance to selenomonas ruminantium in their cell size and in their formation of tufts of flagella, they more closely resemble butyrivibrio fibrisolvens in the subpolar location of their flagella, in their guanine + cytosine content, and in most biochemical characteristics, including butyrate formation. cells of these strains stain gram ...19892743214
effects of methanol on the growth of gastrointestinal anaerobes.the effects of methanol on the growth of representative, predominant, anaerobic gut bacteria were studied. growth yields and rates were determined in a base medium to which methanol was added to produce media with methanol concentrations varying, in twofold steps, over a concentration range of 0.01 to 25%, by volume. the growth of many of the organisms was completely inhibited by a methanol concentration equal to, or less than, 6.2%. isolates representing cellulolytic species were completely inh ...19892743215
the dilation of arterioles in frog submaxillaris muscle caused by visible light.transillumination of submaxillaris muscle with incandenscent lamp led to arteriolar dilation if the illuminance exceeded 1-2 x 10(3) lux or reached the time-mean value of 3 x 10(4) lux when a flash lamp was used (pulse duration 10 microseconds, repetition rate 50 s-1). the temperature increase within a light spot on the muscle did not exceed 0.1 degrees c, visible light being the only reason of the dilation of arterioles. at 6.3 x 10(4) lux (incandescent lamp, green filter) the latency of dilati ...19892786511
use of antibiotic resistance mutations to track strains of obligately anaerobic bacteria introduced into the rumen of sheep.selective plating procedures were used to follow the fate of rifampicin-resistant mutant strains of the obligately anaerobic species bacteroides multiacidus and selenomonas ruminantium after their introduction at numbers around 10(7)/ml into the rumen of sheep. bacteroides multiacidus strain f100 showed an initially rapid rate of loss (49%/h) but subsequently numbers declined more gradually approaching the limits of detection (less than 10(3)/ml) after 100 h. viable cell numbers also decreased i ...19892808185
effect of dietary copper sulfate, aureo sp250, or clinoptilolite on ureolytic bacteria found in the pig large intestine.the predominant ureolytic bacteria in the pig large intestine were determined while growing pigs were fed a basal diet or basal diet plus copper sulfate, aureo sp250, or clinoptilolite. fecal samples were collected from four pigs fed each diet at 3, 9, and 14 weeks and analyzed for total colony counts and percent ureolytic bacteria. fecal urease activity, ammonia nitrogen, and identity of the ureolytic bacteria were determined at 14 weeks. copper sulfate and aureo sp250 reduced the number of ure ...19872823707
isolation and characterization of a temperate bacteriophage from the ruminal anaerobe selenomonas ruminantium.a temperate bacteriophage was obtained from an isolate of the ruminal anaerobe selenomonas ruminantium. clear plaques that became turbid on further incubation occurred on a lawn of host bacteria. cells picked from a turbid plaque produced healthy liquid cultures, but these often lysed on storage. mid-log-phase liquid cultures incubated with the bacteriophage lysed and released infectious particles with a titer of up to 3 x 10(7) pfu/ml. a laboratory strain of s. ruminantium, hd-4, was also sensi ...19882843096
[a case of hepatocellular carcinoma with intratumoral gas formation detected by plain abdominal x-ray film]. 20102846919
human pharmacokinetic and pharmacodynamic studies on ro31-1118, a new beta-adrenoceptor antagonist.the pharmacokinetic and pharmacodynamic effects of ro31-1118 were examined in groups of healthy volunteers. in three subjects given 10 mg of [14c]-ro31-1118 orally, peak levels of radioactivity (84 +/- 5 ng/ml) were 16 times those of the parent drug (approximately 5 ng/ml). very little parent drug was recovered in the urine, although recovery of total radioactivity was nearly 80% in the urine by day 5. in five subjects studied after both oral and intravenous administration of 20 mg ro31-1118 the ...19852859047
effect of phenobarbital on the gamma-glutamyltranspeptidase activity and the remodeling of nodules induced by the initiation-selection model.the effect of subsequent administration of phenobarbital on the gamma-glutamyltranspeptidase (ggt) activity and on the remodeling of nodules induced by the solt-farber procedure was examined in rats. ggt-nodules were initiated by diethylnitrosamine (dena) followed by selection with 2-acetylaminofluorene (2aaf) in the diet and a partial hepatectomy (ph). phenobarbital (500 ppm in the drinking water), administered to rats that were previously treated according to the solt-farber procedure, (1) inc ...19852860967
[management of degenerative changes in the femoro-patellar joint]. 20102863426
[clinical trials in norway]. 20102916210
treatment of the young child with postoperative central diabetes insipidus.a continuous intravenous infusion of aqueous vasopressin (dosage range, 1.0 to 3.0 mu/kg/h) was administered to two patients (respective ages, 2 weeks and 3 years 1 month) who had postoperative central diabetes insipidus to determine if this mode of therapy is helpful in the very young patient. in both patients the polyuria and serum hyperosmolality were corrected. these findings suggest that an intravenous infusion of aqueous vasopressin can provide satisfactory control of the polyuria and elec ...20102916492
[2 cases of pneumoblastoma with pulmonary metastases responsive to chemotherapy]. 20102950479
[nephro-colo-jejunal fistula. a rare complication of percutaneous nephrolithotomy]. 20102950500
plural medicine in sri lanka: do ayurvedic and western medical practices differ?in sri lanka, as in india, two formally structured systems of medicine exist side by side. while western-style biomedicine is believed to be useful, ayurvedic medicine is well established and commonly used. underlying one explanation for the persistence of such plural medical systems is a functional theory, suggesting that each system is used for different treatments, diseases, or for the ideological, linguistic or social characteristics of the physician. in part, ayurvedic and western medicine ...20102976194
chemical derivatization of ca2+-pump protein from skeletal muscle with n-substituted maleimides and 5-(2-iodoacetamidoethyl)aminonaphthalene 1-sulfonate. 20102976460
[surgery in the treatment of patients with small cell lung cancer].the data on surgical and combined treatment of 52 cases of small cell lung cancer are presented and the end results are evaluated. the findings on survival time make the case for surgery as a component of combined treatment of tumor. the best results were obtained in cases of stage i and ii tumor, absence of metastases into intrathoracic lymph nodes, tumor invasion limited to bronchopulmonary lymph nodes or the root of the lung, intermediate cell pattern of small cell lung cancer and surgery fol ...20102998088
cadaverine covalently linked to a peptidoglycan is an essential constituent of the peptidoglycan necessary for the normal growth in selenomonas ruminantium.cadaverine links covalently to the d-glutamic acid residue of the peptidoglycan in selenomonas ruminantium, a strictly anaerobic gram-negative bacterium (kamio, y., itoh, y., and terawaki, y. (1981) j. bacteriol. 146, 49-53). this report clarifies a physiological function of cadaverine in this organism by using dl-alpha-difluoromethyllysine, which had previously been shown to be a selective irreversible inhibitor of lysine decarboxylase of mycoplasma dispar (pösö, h., macann, p.p., tanskanen, r. ...19863084485
cerebral perfusion pressure: a hemodynamic mechanism of mannitol and the postmannitol hemogram.sixteen patients each received infusions of 1 g of mannitol per kg over 5 to 10 minutes, and serial determinations of intracranial pressure (icp), systemic arterial blood pressure (sabp), central venous pressure, cerebral perfusion pressure (cpp), hematocrit, hemoglobin, serum na+, k+, osmolarity, and fluid balance were carried out for 4 hours. urine output was replaced volume for volume with 5% dextrose in 0.45% nacl solution. we tested the hypothesis that patients with high (greater than or eq ...20103116451
susceptibility and resistance of ruminal bacteria to antimicrobial feed additives.susceptibility and resistance of ruminal bacterial species to avoparcin, narasin, salinomycin, thiopeptin, tylosin, virginiamycin, and two new ionophore antibiotics, ro22-6924/004 and ro21-6447/009, were determined. generally, antimicrobial compounds were inhibitory to gram-positive bacteria and those bacteria that have gram-positive-like cell wall structure. mics ranged from 0.09 to 24.0 micrograms/ml. gram-negative bacteria were resistant at the highest concentration tested (48.0 micrograms/ml ...19873116929
factors affecting the rate of breakdown of bacterial protein in rumen fluid.1. the cellular proteins of butyrivibrio fibrisolvens, lactobacillus casei, megasphaera elsdenii, selenomonas ruminantium and streptococcus bovis were labelled by growth in the presence of l-[14c]leucine, and the breakdown of labelled protein was measured in incubations of these bacteria with rumen fluid to which unlabelled 5 mm-l-leucine was added. the rate of protein breakdown was estimated from the rate of release of radioactivity into acid-soluble material. 2. protein breakdown occurred at d ...19873118940
fermentation of xylans by butyrivibrio fibrisolvens and other ruminal bacteria.the ability of butyrivibrio fibrisolvens and other ruminal bacteria (6 species, 18 strains) to ferment a crude xylan from wheat straw or to ferment xylans from larchwood or oat spelts was studied. liquid cultures were monitored for carbohydrate utilization, cell growth (protein), and fermentation acid production. b. fibrisolvens 49, h17c, actf2, and d1 grew almost as well on one or more of the xylans as they did on cellobiose-maltose. b. fibrisolvens 12, r28, a38, x10c34, ard22a, and x6c61 exhib ...19873124741
modification of arachidonic metabolism by flavonoids.the pharmacological effects of many plants depend on their flavonoid constituents. this review is concerned with the influence of flavonoids on arachidonic metabolism as the possible mechanism of action of such natural products. special attention has been paid to structure-activity relationships since this knowledge may allow the synthesis of more potent and selective analogs with therapeutic applications.20113126368
[the occurrence and properties of selenomonas ruminantium bacteria in calves during the time of milk feedings].the occurrence, morphological properties, urease and alpha-amylase activities of selenomonas ruminantium were investigated in calves in the period of milk nutrition. the average number of the organisms was found to range between 10(7) and 10(8) per 1 millilitre of rumen contents. the alpha-amylase activity of the selenomonas strains ranged from 0.1 to 8.8 ncat.ml-1 whereas their urease activity ranged from 6.3 to 261 ncat.ml-1 of nutrient medium. the presence of morphologically typical selenomon ...19883129854
steele-richardson-olszewski syndrome. brain energy metabolism, blood flow and fluorodopa uptake measured by positron emission tomography.brain function was measured in 5 patients with clinically diagnosed steele-richardson-olszewski syndrome using positron emission tomography and tracers of dopamine metabolism, blood flow and oxygen metabolism. a global decrease in blood flow and oxygen utilization compared with normal values was found but the decrease was more marked in the frontal regions. the degree of impairment in oxygen utilization in the frontal region paralleled roughly the duration of the disease. blood flow was impaired ...20113133078
intravenous immune globulin in symptomatic paediatric human immunodeficiency virus infection.seven paediatric patients with symptomatic human immunodeficiency virus (hiv) infection were prospectively studied for 6-24 months after the start of intravenous immune globulin (ivig) therapy. there was substantial clinical benefit during ivig treatment with marked reduction of febrile and infectious episodes, normalization of physical and psychomotor development, and absence of mortality. the immunologic monitoring revealed some discrete objective improvements. the results of this study favour ...19883134244
pathway and sites for energy conservation in the metabolism of glucose by selenomonas ruminantium.on the basis of enzyme activities detected in extracts of selenomonas ruminantium hd4 grown in glucose-limited continuous culture, at a slow (0.11 h-1) and a fast (0.52 h-1) dilution rate, a pathway of glucose catabolism to lactate, acetate, succinate, and propionate was constructed. glucose was catabolized to phosphoenol pyruvate (pep) via the emden-meyerhoff-parnas pathway. pep was converted to either pyruvate (via pyruvate kinase) or oxalacetate (via pep carboxykinase). pyruvate was reduced t ...19883141385
gene-specific differences in the aflatoxin b1 adduction of chicken erythrocyte chromatin.mature and immature chicken erythrocyte nuclei were treated with activated aflatoxin b1 (2,3-dichloroaflatoxin b1), producing covalently bound dna adducts. this reaction produces alkali-labile sites in the dna which can be identified by using a variation of the maxam-gilbert sequencing procedure. we determined the aflatoxin b1 accessibility of defined regions of the erythroid genome by using different specific probes and monitoring the disappearance of similar-sized fragments generated by restri ...19883142684
time-averaged concentration--time-averaged deviation: a new concept in mathematical assessment of dialysis adequacy. 20113147432
a comparison of two survey measures of health status.health service planning requires information on levels of health and illness in the population. surveys, such as the british general household survey (ghs) rely on self-reports of health, illness and restriction, but interpretation of results is problematic. multi-item measures such as the nottingham health profile (nhp) tap different aspects of health and allow respondents less freedom to define health and illness. in a survey of 1862 adults, health questions from the ghs and the nhp were used, ...20113175710
[sections in harnosand conducted a survey: we stick ourselves, get bitten and get blood on the skin]. 20113176627
esterase activities in butyrivibrio fibrisolvens strains.thirty strains of butyrivibrio fibrisolvens isolated in diverse geographical locations were examined for esterase activity by using naphthyl esters of acetate, butyrate, caprylate, laurate, and palmitate. all strains possessed some esterase activity, and high levels of activity were observed with strains 49, h17c, s2, actf2, and lm8/1b. esterase activity also was detected in other ruminal bacteria (bacteroides ruminicola, selenomonas ruminantium, ruminobacter amylophilus, and streptococcus bovis ...19883178205
regulation of carbon flow in selenomonas ruminantium grown in glucose-limited continuous culture.we have applied a model that permits the estimation of the sensitivity of flux through branch point enzymes (d. c. laporte, k. walsh, and d. e. koshland, j. biol. chem. 259:14068-14075, 1984) in order to analyze the control of flux through the lactate-acetate branch point of selenomonas ruminantium grown in glucose-limited continuous culture. at this branch point, pyruvate is the substrate of both the nad-dependent l-(+)-lactate dehydrogenase (ldh) and the pyruvate:ferredoxin oxidoreductase (pfo ...19883182729
effects of the ionophores monensin and tetronasin on simulated development of ruminal lactic acidosis in vitro.a continuous coculture of four ruminal bacteria, megasphaera elsdenii, selenomonas ruminantium, streptococcus bovis, and lactobacillus sp. strain lb17, was used to study the effects of the ionophores monensin and tetronasin on the changes in ruminal microbial ecology that occur during the onset of lactic acidosis. in control incubations, the system simulated the development of lactic acidosis in vivo, with an initial overgrowth of s. bovis when an excess of glucose was added to the fermentor. la ...19883223764
functionally polarized layers formed by epidermal cells on a permeable transparent collagen film.rat epidermal cells were cultured on a transparent collagen film, which was permeable to low mr substances. then the cells were bathed in both media (apical and basal), which were separated by the collagen membrane. the cells formed a multi-layered epidermal sheet with well-developed structures of desmosomes. this sheet on a permeable support was found to be an effective permeability barrier for glucose and amino acids. the epidermal layer showed functional polarity for the uptake and excretion ...19883255753
autoimmune mrl/lpr mice sera contain igg with interleukin 3-like activity.the spleen cells, thymocytes, and bone marrow cells of autoimmune mrl/mp-lpr/lpr (mrl/lpr) mice do not constitutively produce interleukin 3 (il-3), but these mice had il-3-like activity in their sera. mrl/lpr sera supported the growth of the il-3-dependent cell lines fdc-p2 and da-1 but not the growth of il-2-dependent t-572 cells. this il-3-like activity increased with age. biochemical analysis of the mrl/lpr sera by anion-exchange chromatography, gel filtration on a superose 12 column, the bin ...20123257242
how "fine" is incisional biopsy of melanoma. 20123273029
[hepatic and peripheral insulin resistance as a cause of hyperglycemia in non-insulin-dependent (type 2) diabetes mellitus: a review].in diabetic patients, fasting hyperglycaemia is attributed to both, reduced clearance by peripheral tissues and augmented endogenous glucose release. in normal-weight, non-insulin-dependent diabetic patients, basal hepatic glucose production (hgp) was determined by means of tracer kinetic analysis. hgp was enhanced to 3.00 +/- 0.20 mg/kg x min as compared to 1.90 +/- 0.05 in healthy, non-diabetic subjects, even though hyperglycaemia and fasting hyperinsulinaemia prevailed. hgp correlated positiv ...20113296467
the microcirculatory society eugene m. landis award lecture. capillaries and cowboys. 20123302620
further evidence that prolactin does not affect gonadotropin release at pituitary level.in a primary monolayer cell culture of the anterior pituitary from mature male rats the effects of exogenous rprl (rprl exog.) and endogenously secreted rprl (rprl endog.) on basal and lhrh stimulated lh secretion were investigated. in pilot studies basal prl- and lh secretion as well as influence of various lhrh concentrations (10(-1)-10(+3) ng/ml) on prl- and lh release were observed. the influence of exogenous rprl was studied at various concentrations (50-500 ng/ml) and with preincubation pe ...19873323020
alterations in aortic endothelial cell morphology and cytoskeletal protein synthesis during cyclic tensional deformation.previous studies have shown that bovine aortic endothelial cells (ecs) in culture respond to repetitive tensional deformation with an increase in deoxyribonucleic acid synthesis and cell proliferation. this study was designed to determine whether cyclic tensional deformation of ecs in vitro induces different morphologic or protein synthetic responses. ecs from passages 6 through 9 were seeded in 35 mm2 well silicone rubber plates at 2 x 10(5) cells/well and allowed to attach for 24 hours. the ex ...19883336119
[nosocomial infections of the urinary tract in urology patients].researches effected in different nations have revealed that 5-12% of all hospitalized patients was affected with a nosocomial infection and that almost 1/3 of these were urinary tract infections. the hospitalized urological patients have a considerable proneness to contract a nosocomial urinary tract infection, because they are subjected to transurethral or suprapubic bladder catheterisms, to transurethral surgery and to the endoscopies, that are promoting maneuvers the urinary tract infections. ...19883340313
differentiation of ruminal bacterial species by enzyme-linked immunosorbent assay using egg yolk antibodies from immunized chicken hens.cross-reactivity among four species of ruminal bacteria was examined by using egg yolk antibodies from immunized leghorn laying hens and an enzyme-linked-immunosorbent assay. the effects of the four species on the hens were compared on various days postimmunization. hens injected with the same bacterial species had similar apparent antibody levels over the entire postimmunization period, but only bacteroides ruminicola b1(4) and selenomonas ruminantium d antigens elicited early increases in appa ...19883355145
effects of potassium ion concentrations on the antimicrobial activities of ionophores against ruminal anaerobes.the antimicrobial activities of monensin and lasalocid against representative strains of ruminal bacteria were evaluated in medium containing three different concentrations of potassium (1.3, 7.9, or 23.3 mm). the growth of eubacterium ruminantium was inhibited by low concentrations of ionophores (less than or equal to 0.16 mg/liter), while the strain of streptococcus bovis tested was resistant to high concentrations of ionophores (40 mg/liter) at all potassium concentrations tested. the mics of ...19873426214
[rapid decisions in the situation of dichotic choice: an integrated approach which takes into account onset snd execution of the response]. 20123502909
fodrin is part of a filamentous cortical sheath of the detergent resistant cytoskeleton of cultured cells before and after cytochalasin treatment.cytoskeletons of cultured cells prepared under mild conditions in the presence of "stabilization' buffer contain most of the fodrin present in the cells. the fodrin in these cytoskeletons was localized by immunofluorescence microscopy and found to be present in a cortical sheath of fine filaments. in general, the filamentous distribution showed no correspondence with actin bundles as revealed by double-label fluorescence microscopy. however, in cells with large and abundant stress fibers, some c ...19863514218
patterns of drug usage in colonial america. 20113550544
t-2 toxin metabolism by ruminal bacteria and its effect on their growth.the effect of t-2 toxin on the growth rates of different bacteria was used as a measure of its toxicity. toxin levels of 10 micrograms/ml did not decrease the growth rate of selenomonas ruminantium and anaerovibrio lipolytica, whereas the growth rate of butyrivibrio fibrisolvens was uninhibited at toxin levels as high as 1 mg/ml. there was, however, a noticeable increase in the growth rate of b. fibrisolvens ce46 and ce51 and s. ruminantium in the presence of low concentrations (10 micrograms/ml ...19873579272
a novel technique for measuring hypothalamic and pituitary hormone secretion rates from collection of pituitary venous effluent in the normal horse.we have described a novel technique for collecting pituitary venous effluent in the horse by placing a cannula in the intercavernous sinus close to the outlet of the pituitary veins using a venous pathway unique to equids. cannula placement and blood collection are carried out painlessly in fully conscious, ambulatory, unstressed animals. there is no interference to hypothalamic, pituitary or target organ function. the blood collected contains readily measurable concentrations of gonadotrophin-r ...19873585231
alkaline reflux gastritis: late results on a controlled trial of diagnosis and treatment. 20133592819
[formaldehyde allergies]. 20133645758
structural specificity of diamines covalently linked to peptidoglycan for cell growth of veillonella alcalescens and selenomonas ruminantium.putrescine and cadaverine are essential constituents of the peptidoglycan of veillonella alcalescens, veillonella parvula, and selenomonas ruminantium and are necessary for the growth of these organisms (y. kamio and k. nakamura, j. bacteriol. 169:2881-2884, 1987, and y. kamio, h. pösö, y. terawaki, and l. paulin, j. biol. chem. 261:6585-6589, 1986). in this study, the structural specificity of the diamine requirement for normal cell growth of these bacteria was examined by using a series of dia ...19873654585
[cardiac autonomic neuropathy in type i diabetics]. 20133660679
facile synthesis of 2-[(3-aminopropyl)thio]adenosine 5'-diphosphate: a key intermediate for the synthesis of molecular probes of adenosine 5'-diphosphate function.adenosine 5'-diphosphate (adp) and, uniquely, its c-2 derivatized analogues are able to induce platelet activation. we here report the synthesis of 2-[(3-aminopropyl)thio]-adp from adp itself via 1,n6-etheno-adp. 2-[(3-aminopropyl)thio]-adp induced platelet aggregation with a potency about one-seventh that of adp itself and should prove a useful intermediate in the synthesis of other probes of platelet function.20133669009
serum tryptophan and cataract.total serum tryptophan concentrations were measured in 74 cataractous patients and 124 controls. no significant differences were observed in the mean values for the cataractous and normal subjects, 11.4 +/- 2.6 and 11.3 +/- 2.2 micrograms/ml respectively, or in the range of values, 7.4-17.1 and 7.3-17.4 micrograms/ml respectively. these results do not support previous reports that serum tryptophan is elevated in cataractous subjects.20133675914
purification of a nickel-containing urease from the rumen anaerobe selenomonas ruminantium.urease was purified 592-fold to homogeneity from the anaerobic rumen bacterium selenomonas ruminantium. the urease isolation procedure included a heat step and ion-exchange, hydrophobic, gel filtration, and fast protein liquid chromatography. the purified enzyme exhibited a km for urea of 2.2 +/- 0.5 mm and a vmax of 1100 mumol of urea min-1 mg-1. the molecular mass estimated for the native enzyme was 360,000 +/- 50,000 daltons, whereas a subunit value of 70,000 +/- 2,000 daltons was determined. ...19863711113
black teenage fathers: what happens when the child is born?this study explored the changes in the lives of teenage fathers, from prenatally to 9 and 18 months postpartum, and compared them to a group of nonfather peers. at 18 months postpartum, more fathers were heads of households and fewer were still in school, although there were no differences in employment status. although only 7.5% of the teenage parents were married, 37% lived together or had daily contact; only three had contact less often than weekly. in the 18 months since the first interview, ...20133725487
stoichiometry of glucose and starch splitting by strains of amylolytic bacteria from the rumen and anaerobic digester.the stoichiometry of glucose and starch splitting by the amylolytic bacteria streptococcus bovis, selenomonas ruminantium, butyrivibrio fibrisolvens, eubacterium ruminantium and clostridium sp. was followed. there were many differences in the ratios of metabolites and in growth yields, as well as in the cell composition, between the growth on glucose and starch. the bacteria employ different nutritional strategies with respect to both energy sources.19863759723
heat production by ruminal bacteria in continuous culture and its relationship to maintenance energy.selenomonas ruminantium hd4 and bacteroides ruminicola b(1)4 were grown in continuous culture with glucose as the energy source, and heat production was measured continuously with a microcalorimeter. because the bacteria were grown under steady-state conditions, it was possible to calculate complete energy balances for substrate utilization and product formation (cells, fermentation acids, and heat). as the dilution rate increased from 0.04 to 0.60 per h, the heat of fermentation declined from 1 ...19863782021
phosphoenolpyruvate-dependent phosphorylation of hexoses by ruminal bacteria: evidence for the phosphotransferase transport system.six species of ruminal bacteria were surveyed for the phosphoenolpyruvate (pep)-dependent phosphorylation of glucose. selenomonas ruminantium hd4, streptococcus bovis jb1, and megasphaera elsdenii b159 all showed significant activity, but butyrivibrio fibrisolvens 49, bacteroides succinogenes s85, and bacteroides ruminicola b1(4) showed low rates of pep-dependent phosphorylation and much higher rates in the presence of atp. s. ruminantium hd4, s. bovis jb1, and m. elsdenii b159 also used pep to ...19863789722
hemodynamic effects of sodium in uremia. 19873794516
the education debate. the shape of things to come. 20133847938
[postural exercise therapy for ventilated patients]. 20133851495
theoretical mechanisms for synthesis of carcinogen-induced embryonic proteins: xiv. mutational and non-mutational mechanisms as subsets of a more general mechanism. part c. a defined cancer mutation.the results of applying a mechanism of ethionine-induced embryonic gene derepressions to explain similar features found in hereditary tyrosinemia have been extended to a well defined cancer mutation. in all three cases, the described mechanism is compatible with the explanation for the etiology of embryonic like phenotypic expressions in cells and potentially for the carcinogenic process. the essence of the formulated process for a human bladder carcinoma mutation in the ras gene for a protein p ...19853853079
evaluation of interferon treatment of cervical intraepithelial neoplasia. 20143885665
[a case of resected renal cell carcinoma with massive pancreatic metastases].a case of resected renal cell carcinoma with massive pancreatic metastases is reported. a 72-year-old man was diagnosed as suffering from obstructive jaundice. massive pancreatic tumor and left renal tumor were shown by several kinds of radiological examination. total pancreatectomy and left nephrectomy were performed. postoperatively this case was histologically diagnosed as renal cell carcinoma with massive pancreatic metastases. very few cases of resected renal cell carcinoma with pancreatic ...19853981807
intracoronary naloxone in hemorrhagic shock: dose-dependent stereospecific effects.treatment with naloxone improves cardiovascular function and survival in a variety of shock models, and numerous sites and mechanisms for its action have been proposed. data presented in this article support the hypothesis that in hemorrhagic shock naloxone exerts its beneficial hemodynamic effects by acting primarily at cardiac opiate receptors. naloxone or its stereoisomer (d-naloxone) were administered intravenously (iv) and directly into the coronary circulation (ic) in dogs anesthetized wit ...19854025562
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