Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| pilus elisa and an anamnestic test for the diagnosis of virulent ovine footrot and its application in a disease control program in nepal. | the immunological memory (anamnestic) responses in sheep recovered from virulent footrot (vfr) can be aroused by subcutaneous injection of outer membrane protein (omp) antigens of dichelobacter nodosus. the magnitude of this response is directly correlated to the highest antibody response attained during infection and memory lasts at least a year after recovery from vfr. however, some older animals show non-specific responses to omp antigens. in this study an evaluation of d. nodosus pilus antig ... | 2001 | 11230927 |
| pseudomonas stutzeri has two closely related pila genes (type iv pilus structural protein) with opposite influences on natural genetic transformation. | pseudomonas stutzeri has type iv pili for which the pila gene (here termed pilai) provides the structural protein and which are required for dna uptake and natural genetic transformation. downstream of pilai we identified a gene, termed pilaii, coding for a deduced protein with a size similar to that of pilai with 55% amino acid sequence identity and with a typical leader peptide including a leader peptidase cleavage site. fusions to lacz revealed that pilaii is expressed only about 10% compared ... | 2001 | 11244078 |
| dichelobacter nodosus serotype m fimbrial subunit gene: implications for serological classification. | dichelobacter nodosus fimbrial subunit gene (fima) from a serotype m strain (m-spahl) was investigated in this study. a primer set targeting the relatively conserved fima regions and based on the published sequence from nepalese serogroup m isolates (nepalese m), failed to amplify the fima of m-spahl. however, when the downstream primer was substituted with a primer that is specific for other serogroups of d. nodosus, the fima was successfully amplified. cloning followed by dna sequencing, revea ... | 2001 | 11267795 |
| rapid and accurate typing of dichelobacter nodosus using pcr amplification and reverse dot-blot hybridisation. | here we describe an approach to genotyping d. nodosus, based on variation in the fimbrial subunit gene (fima), which uses polymerase chain reaction (pcr) amplification and hybridisation to immobilised oligonucleotides (pcr/oligotyping). the variable region of d. nodosus fima, amplified and labelled with digoxigenin (dig) in a single multiplex pcr amplification, was hybridised to a panel of group- and type-specific poly-dt tailed oligonucleotides that were immobilised on a nylon membrane strip. a ... | 2001 | 11295335 |
| comparison of erythromycin and oxytetracycline for the treatment of ovine footrot. | a microbiological study of 25 cases of ovine footrot was performed. cultures belonging to dichelobacter nodosus were isolated in 48% of the sampled animals. the sensitivity of the 99 strict anaerobic bacterial isolates to 5 antibiotics (penicillin g, amoxycillin, spiramycin, erythromycin and oxytetracycline) was studied. the percentage of resistant cultures was in all cases higher than 30%. the efficacy of erythromycin and oxytetracycline in the treatment of ovine footrot was studied. to conduct ... | 2001 | 11402641 |
| the type iv fimbrial subunit gene (fima) of dichelobacter nodosus is essential for virulence, protease secretion, and natural competence. | dichelobacter nodosus is the essential causative agent of footrot in sheep. the major d. nodosus-encoded virulence factors that have been implicated in the disease are type iv fimbriae and extracellular proteases. to examine the role of the fimbriae in virulence, allelic exchange was used to insertionally inactivate the fima gene, which encodes the fimbrial subunit protein, from the virulent type g d. nodosus strain vcs1703a. detailed analysis of two independently derived fima mutants revealed t ... | 2001 | 11443078 |
| eradication of ovine footrot by repeated daily footbathing in a solution of zinc sulphate with surfactant. | to investigate the effect on ovine footrot of repeated daily footbathing in a solution of zinc sulphate with surfactant. | 2001 | 11491224 |
| evaluation of equine immunoglobulin specific for rhodococcus equi virulence-associated proteins a and c for use in protecting foals against rhodococcus equi-induced pneumonia. | to determine whether purified equine immunoglobulin specific for rhodococcus equi virulence-associated proteins a and c (vapa and vapc) can confer passive protection against r. equi-induced pneumonia in foals. | 2001 | 11497456 |
| genomic islands of dichelobacter nodosus. | 2002 | 12012869 | |
| characterization and expression of a novel porphyromonas gingivalis outer membrane protein, omp28. | we report the characterization of a porphyromonas gingivalis gene, designated omp28, encoding a protein that we have previously purified and characterized as a 28-kda outer membrane protein. the deduced amino acid sequence of the omp28 open reading frame displayed an outer membrane leader sequence and lipoprotein attachment site but did not exhibit any significant overall sequence identity with protein sequences in the databases. a small stretch of amino acids (19 residues) exhibits 50% sequence ... | 2002 | 12030966 |
| diagnosis of footrot in goats: application of elisa tests for response to antigens of dichelobacter nodosus. | goats are an important natural host for footrot and are infected with dichelobacter nodosus that have virulence characteristics similar to those of sheep strains. however, the humoral response of goats to d. nodosus antigens and the possibility of a serological diagnosis of footrot in goats have not been studied. with the aim of evaluating a diagnostic elisa test, we investigated the primary immune response of goats to experimental and natural infection, the memory response in recovered animals, ... | 2002 | 12052334 |
| eradication of virulent footrot from sheep and goats in an endemic area of nepal and an evaluation of specific vaccination. | programmes based on the identification and treatment of cases and the culling of animals refractory to treatment had failed to eradicate virulent footrot from two districts in the western region of nepal. from 1993 to 1996 vaccination against two endemic virulent strains of dichelobacter nodosus was tested for its potential to contribute to the eradication of footrot from the region. only sheep and goats which had been free of signs of footrot at three inspections at monthly intervals before the ... | 2002 | 12243270 |
| serogroup specific single and multiplex pcr with pre-enrichment culture and immuno-magnetic bead capture for identifying strains of d. nodosus in sheep with footrot prior to vaccination. | the identification of dichelobacter nodosus present in a flock is a prerequisite to specific (autogenous) vaccination. current methods of identification of the serogroup present in a population requires that the organisms be isolated, identified visually in mixed culture on streak plates, subcultured to purify and subjected to antigenic analysis. this process takes at least 3 to 4 weeks. this study describes the development of a simple and rapid serogroup specific pcr test for d. nodosus. a comm ... | 2002 | 12270270 |
| transformation-mediated serogroup conversion of dichelobacter nodosus. | dichelobacter nodosus is the essential causative agent of footrot in sheep. the type iv fimbriae of d. nodosus are required for virulence, are highly immunogenic and immunoprotective, and can be divided into 10 major serogroups. fimbrial variation has been postulated to have arisen because of genetic recombination within the fimbrial gene region perhaps as a means of evading the immune response invoked by infection. to show that antigenic variation in these fimbriae could occur after natural tra ... | 2003 | 12488080 |
| aetiology of ovine footrot in the portuguese region of alto alentejo. | in this work, we found it appropriate to carry out a study directed towards isolating and identifying the entailed microorganisms which trigger off footrot in sheep, placing special emphasis on the serotipification of the different dichelobacter nodosus species. with this goal in mind four flocks from the portuguese region of 'alto alentejo' were selected, all of them had one common feature: their main health problem was ovine footrot. we also set out to determine the elastolitic capacity of iso ... | 2003 | 12667188 |
| ovine periodontitis as a potential model for periodontal studies. cross-sectional analysis of clinical, microbiological, and serum immunological parameters. | : to investigate infection and host immunity patterns in sheep with naturally occurring "broken-mouth" periodontitis. | 2003 | 12702113 |
| clinical and radiographic findings in six cattle with cervical diskospondylitis. | 2003 | 12790169 | |
| contagious ovine digital dermatitis. | 2003 | 12790246 | |
| genome sequences of two closely related vibrio parahaemolyticus phages, vp16t and vp16c. | two bacteriophages of an environmental isolate of vibrio parahaemolyticus were isolated and sequenced. the vp16t and vp16c phages were separated from a mixed lysate based on plaque morphology and exhibit 73 to 88% sequence identity over about 80% of their genomes. only about 25% of their predicted open reading frames are similar to genes with known functions in the genbank database. both phages have cos sites and open reading frames encoding proteins closely related to coliphage lambda's termina ... | 2003 | 14563879 |
| failure to eradicate ovine footrot associated with dichelobacter nodosus strain a198 by repeated daily footbathing in zinc sulphate with surfactant. | to investigate the effect of repeated daily footbathing in zinc sulphate on virulent ovine footrot associated with s1, u1 and u5 zymogram types of dichelobacter nodosus, including the highly virulent s1 strain a198. | 2003 | 15084013 |
| eradication of footrot of lesser clinical severity (intermediate footrot). | to determine if intermediate footrot (ifr) can be eradicated from a flock of sheep by inspection and culling of cases during a non-transmission period and if prior antibiotic treatment or vaccination increases the likelihood of eradication. | 2003 | 15086111 |
| molecular detection and characterization of dichelobacter nodosus in ovine footrot in india. | dichelobacter nodosus was detected in three clinical cases of ovine footrot in kashmir, india. the detection was done by pcr in three clinical specimens directly, without isolating the organism, using species-specific 16s rdna primers. positive results were indicated by amplification of a 783 bp product. all the three samples were subjected to serogrouping by multiplex pcr using group (a-i) specific primers. all the three samples revealed the presence of serogroup b of d. nodosus by yielding a s ... | 2004 | 15294315 |
| minimum inhibitory concentrations for 25 selected antimicrobial agents against dichelobacter nodosus and fusobacterium strains isolated from footrot in sheep of portugal and spain. | the agar dilution method was used to determine the inhibitory activity of 25 antimicrobial agents against 69 strains of dichelobacter nodosus and 108 strains of the genus fusobacterium, all of which were isolated from 90 clinical cases of ovine footrot between october 1998 and november 2000. in the case of the micro-organisms belonging to the genus fusobacterium, the six beta-lactams studied (benzyl penicillin, ampicillin, cloxacillin, cefadroxil, cefuroxime and cephalexine) proved to be, in gen ... | 2004 | 15330985 |
| comparative study of biochemical methods for the identification of dichelobacter nodosus. | the aim of this study was to compare four identification procedures to detect dichelobacter nodosus and develop a rapid, simple and effective method to identify d. nodosus strains isolated from cases of ovine footrot. the four methods used were: (a) the classic guidelines set down by holdeman et al. (1977) and summanen et al. (1993) which are based on gas liquid chromatography (glc) and different biochemical tests, this method was considered as landmark; (b) baron and citron's flowchart for the ... | 2004 | 15379442 |
| effectiveness of different adjuvants in stimulating dichelobacter nodosus antibody in sheep vaccinated against ovine footrot. | this research consists of an evaluation of the effectiveness of different substances administered as adjuvants in the stimulation of humoral immune response induced by the vaccine composed of strains a1, a2 and c of dichelobacter nodosus. to do this, a total of 120 merino sheep were vaccinated and revaccinated. these sheep were selected from a farm located in the region of extremadura (spain), and they were divided into 12 groups of 10 animals each. an additional group with 10 sheep was used as ... | 2004 | 15379443 |
| identification of a dichelobacter nodosus ferric uptake regulator and determination of its regulatory targets. | the expression of iron regulated genes in bacteria is typically controlled by the ferric uptake regulator (fur) protein, a global transcriptional repressor that regulates functions as diverse as iron acquisition, oxidative stress, and virulence. we have identified a fur homologue in dichelobacter nodosus, the causative agent of ovine footrot, and shown that it complements an escherichia coli fur mutant. homology modeling of the d. nodosus fur protein with the recently solved crystal structure of ... | 2005 | 15601721 |
| comparative study of three surgical treatments for two forms of the clinical presentation of bovine pododermatitis. | in this study, 1013 animals showing signs of clinical pododermatitis were examined and divided into five unevenly numbered groups. affected animals in groups i and ii showed only signs of vegetative interdigital pododermatitis. the lesions were surgically removed and either protected with bandages (in group i) or cauterized with incandescent iron (group ii). the animals in groups iii, iv, and v, showed signs of necrotic pododermatitis. these were treated with different protocols after the necrot ... | 2004 | 15604479 |
| current approaches to the management of ovine footrot. | ovine footrot remains the most important cause of lameness in sheep flocks in the uk, despite the existence of proven methods for the control of the disease. recent research suggests that sheep farmers may be unaware of these methods and may allocate greater resources to treatment of footrot rather than to its prevention. foot paring, topical treatments, vaccination and parenteral antibiotic therapy all have a role in treating sheep with advanced footrot infections, but prevention of severe infe ... | 2005 | 15683762 |
| plasmid-borne florfenicol resistance in pasteurella multocida. | a florfenicol-resistant pasteurella multocida isolate from a calf was investigated for the genetic basis of florfenicol resistance and the location of the resistance gene. | 2005 | 15814600 |
| the detection and characterisation of dichelobacter nodosus from cases of ovine footrot in england and wales. | footrot, caused by the strictly anaerobic bacterium dichelobacter nodosus, is the most common cause of lameness in sheep in great britain but problems exist in association with its diagnosis and control. the fastidious nature of d. nodosus means that complex media and several weeks are required for characterisation. an alternative method to simplify and enhance the detection of d. nodosus in clinical samples is therefore highly desirable. in terms of control, anecdotal evidence from the farming ... | 2005 | 15917134 |
| the use of an autogenous dichelobacter nodosus vaccine to eliminate clinical signs of virulent footrot in a sheep flock in bhutan. | an outbreak of virulent footrot was investigated in a flock of 605 merino cross-bred sheep in bhutan. conventional control methods in the preceding eight years had reduced its prevalence from 36-79% in different components of the flock to about 15% overall. only one serogroup (b) of dichelobacter nodosus was identified among 40 isolates cultured from affected sheep. a vaccine prepared from this strain was used in a pilot trial to compare the response of 14 treated and 14 untreated sheep. all aff ... | 2006 | 16019243 |
| the use of melatonin as a vaccine agent. | molecules with immunomodulatory properties determine the magnitude and quality of immune responses specific for the coadministered antigen. melatonin is considered a biological-response modifier of the immune system with broad application in veterinary medicine. in seasonally-breeding animals, the indolamine is able to improve reproductive performance. with the purpose of expanding new advantageous roles for melatonin, we investigated the effect of subcutaneous slow-release melatonin implants in ... | 2005 | 16055232 |
| update on ovine footrot in new zealand: isolation, identification, and characterization of dichelobacter nodosus strains [corrected]. | dichelobacter nodosus, a gram-negative strict anaerobe, is the essential causative agent of ovine footrot. despite its worldwide presence, the disease has significant economic impact in those sheep-farming countries with a temperate climate and moderate to high rainfall, such as new zealand (nz) and australia. in this study, we aimed to isolate, identify, and characterize as many d. nodosus strains as possible from nz farms by using polymerase chain reaction (pcr)-based technology. understanding ... | 2005 | 16280202 |
| the occurrence of treponemes in contagious ovine digital dermatitis and the characterisation of associated dichelobacter nodosus. | contagious ovine digital dermatitis (codd) is a recently recorded, apparently new infection of the ovine hoof, which differs clinically from footrot caused by dichelobacter nodosus and which fails to respond well to accepted treatment practices for footrot. despite the welfare implications of such an infection, very little research has been performed on codd to date and the aetiology remains confused. suggestions have been made that there is a potential role for treponemes in the pathogenesis of ... | 2005 | 16280206 |
| recent footrot outbreak in debrezeit swine farm, central ethiopia. | an outbreak of footrot has occurred in debrezeit swine farm that is located 44 km south east of the capital. among 24 pigs, 75% showed lameness, hot, painful and swollen feet, hemorrhagic bruising of the coronary band, heel erosion, sole ulcers and separation of skin from hoof on hind limbs. bacteriological examination of the specimen from the affected limbs has shown the involvement of staphylococcus, streptococcus and dichelobacter nodosus. the overt clinical lameness and inability to stand or ... | 2005 | 16294005 |
| distribution and prevalence of footrot in bhutan. | the first cases of footrot in bhutan were reported in sheep in 1990 at the national sheep breeding centre (nsbc), which supplies breeding animals to village sheep flocks throughout bhutan. despite the presence of footrot at the centre the distribution of apparently disease-free sheep continued. cases of footrot were reported in village flocks soon after the disease was diagnosed at nsbc. a national survey was designed to establish the distribution and prevalence of footrot in bhutan. this detect ... | 2006 | 16490719 |
| current understanding of the aetiology and laboratory diagnosis of footrot. | footrot is a highly contagious disease of the feet of ruminants caused by the synergistic action of certain bacterial species of which dichelobacter nodosus (d. nodosus) is the main transmitting agent. the infection is specific to sheep and goats, although it has also been reported in cattle, horses, pigs, deer and mouflon. the antigenic diversity of d. nodosus is due to variations in the dna sequence of its fimbrial subunit gene (fima) and provides the basis for classification of the organism i ... | 2006 | 16624707 |
| improved diagnosis of virulent ovine footrot using the inta gene. | footrot is a mixed bacterial infection of the hooves of sheep. the gram-negative anaerobic bacterium dichelobacter nodosus is the principal causative agent, with different strains causing diseases of different severity, ranging from benign to virulent. in australia, in the state of new south wales (nsw), only virulent footrot is subject to regulatory action, including quarantine. however, it is often difficult to distinguish benign footrot from virulent footrot in the initial stages of infection ... | 2006 | 16716540 |
| regulation of type iv fimbrial biogenesis in dichelobacter nodosus. | type iv fimbriae are expressed by several bacterial pathogens and are essential for virulence in dichelobacter nodosus, which causes ovine footrot. we have identified a two-component signal transduction system (pilr/s) and an alternative sigma factor (sigma 54) that were shown by insertional inactivation to be required for the regulation of fimbrial biogenesis in d. nodosus. western blots showed that in both pilr and rpon mutants, fimbrial subunit production was significantly reduced by a proces ... | 2006 | 16788189 |
| activity and cellular localization of amylases of rabbit cecal bacteria. | five 11-week-old rabbits, fed a commercial granulated feed, were slaughtered and cecal starch-degrading bacteria enumerated; total concentration of cultivable bacteria utilizing starch averaged 5.5 x 10(10) cfu/g. the activity and cellular localization of amylases was determined in 9 bacteria identified as actinomyces israeli (strains aa2 and ad4), bacteroides spp. (strain aa3), dichelobacter nodosus (strain aa4), mitsuokella multiacidus (strain aa6), eubacterium spp. (strains aa7 and ab2), clos ... | 2006 | 17007433 |
| aetiology of caprine footrot in extremadura region, spain. | the present study reports on the location of major foci of footrot in goats in the extremadura region of spain by the determination of locally occurring strictly anaerobic microorganisms involved in the pathogenesis and development of this disease. the most commonly isolated microorganisms belonged to the genera dichelobacter, fusobacterium, porphyromonas and prevotella; these were found in conjunction with other species of minor importance. the species most frequently isolated were fusobacteriu ... | 2006 | 17020135 |
| isolation and characterization of dichelobacter nodosus from ovine and caprine footrot in kashmir, india. | footrot is a highly contagious and economically important disease of sheep and goats, caused by dichelobacter nodosus, a slow growing anaerobic gram-negative rod. the current australian antigenic classification system, based on variation in the fimbriae, classifies d. nodosus into at least 10 serogroups (a-i and m) and 18 serotypes. this investigation was intended to determine the serological diversity of d. nodosus in this region of kashmir, india. exudates of footrot lesions were collected fro ... | 2007 | 17250860 |
| detection of dichelobacter nodosus in wild ungulates (capra ibex ibex and ovis aries musimon) and domestic sheep suffering from foot rot using a two-step polymerase chain reaction. | severe keratinous hoof afflictions have been recorded in ibex (capra ibex ibex) since 1995 and more recently in mouflon (ovis aries musimon) in switzerland. based on clinical observations and comparison with diseases known to affect domestic ungulates, it was hypothesized these wild ungulates were affected by foot rot associated with infection with dichelobacter nodosus. dichelobacter nodosus has been shown to be the essential pathogen for initiation and establishment of foot rot, a highly conta ... | 2007 | 17347396 |
| in vitro antimicrobial susceptibility of anaerobic bacteria isolated from caprine footrot. | the in vitro antimicrobial susceptibility of gram-negative anaerobic bacilli commonly isolated from footrot in goats was studied. a total of 97 isolates belonging to the genera dichelobacter, fusobacterium, prevotella, porphyromonas and bacteroides, obtained from clinical cases of footrot in south-western spain between march 2000 and may 2001, were tested against 25 antimicrobial agents comprising beta-lactams, aminoglycosides, macrolides, chloramphenicol, quinolones, lincosamides, sulphonamides ... | 2007 | 17385552 |
| assessment of current knowledge of footrot in sheep with particular reference to dichelobacter nodosus and implications for elimination or control strategies for sheep in great britain. | a brief summary of the currently accepted aetiology and pathogenesis of footrot and interdigital dermatitis in sheep in great britain is presented together with a description of the transmission dynamics of dichelobacter nodosus. approaches that may be successful for control or elimination of the disease are proposed and areas of uncertainty in current knowledge highlighted. research and practice in great britain is compared with that from other countries and the proposal made that the environme ... | 2008 | 17418598 |
| genome sequence and identification of candidate vaccine antigens from the animal pathogen dichelobacter nodosus. | dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. we sequenced its 1.4-mb genome, the smallest known genome of an anaerobe. it differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the geno ... | 2007 | 17468768 |
| type iv fimbrial biogenesis is required for protease secretion and natural transformation in dichelobacter nodosus. | the objective of this study was to develop an understanding of the molecular mechanisms by which type iv fimbrial biogenesis, natural transformation, and protease secretion are linked in the ovine foot rot pathogen, dichelobacter nodosus. we have shown that like the d. nodosus fimbrial subunit fima, the pilin-like protein pile and the fimn, fimo, and fimp proteins, which are homologs of pilb, pilc, and pild from pseudomonas aeruginosa, are essential for fimbrial biogenesis and natural transforma ... | 2007 | 17513472 |
| glycosylation of type-iv fimbriae of dichelobacter nodosus. | dichelobacter nodosus is the causative agent of ovine footrot and the type-iv fimbriae on this bacterium are essential for maintaining its virulence. in this study, we reveal that these fimbriae are glycosylated. this was demonstrated in several ways: by the detection of carbohydrate on fimbrial protein using periodic acid schiff reagent (pas) staining of sds-page gels and by demonstrating enzymatic deglycosylation and by analysis of the amino acid sequences derived from the fima gene, whereby t ... | 2008 | 17681435 |
| analyses of the vrl gene cluster in desulfococcus multivorans: homologous to the virulence-associated locus of the ovine footrot pathogen dichelobacter nodosus strain a198. | major parts of the virulence-associated vrl locus known from the gammaproteobacterium dichelobacter nodosus, the causative agent of ovine footrot, were analyzed in the genome of the sulfate-reducing deltaproteobacterium desulfococcus multivorans. in the genome of d. multivorans 13 of the 19 vrl genes described for d. nodosus are present and highly conserved with respect to gene sequence and order. the vrl locus and its flanking regions suggest a bacteriophage-mediated transfer into the genome of ... | 2007 | 17693723 |
| twitching motility is essential for virulence in dichelobacter nodosus. | type iv fimbriae are essential virulence factors of dichelobacter nodosus, the principal causative agent of ovine foot rot. the fima fimbrial subunit gene is required for virulence, but fima mutants exhibit several phenotypic changes and it is not certain if the effects on virulence result from the loss of type iv fimbria-mediated twitching motility, cell adherence, or reduced protease secretion. we showed that mutation of either the pilt or pilu gene eliminated the ability to carry out twitchin ... | 2008 | 18310333 |
| the complete sequence and functional analysis of panl, the large plasmid of the unicellular freshwater cyanobacterium synechococcus elongatus pcc 7942. | two endogenous plasmids are present in synechococcus elongatus pcc 7942, a model organism for studying photosynthesis and circadian rhythms in cyanobacteria. the large plasmid, panl, was shown previously to be involved in adaptation of s. elongatus cells to sulfur starvation, which provided the first evidence of cellular function of a cyanobacterial plasmid. here, we report the complete sequence of panl, which is 46,366 bp in length with 53% gc content and encodes 58 putative orfs. the panl plas ... | 2008 | 18353436 |
| breeding for resistance to footrot--the use of hoof lesion scoring to quantify footrot in sheep. | so that genetic studies can be undertaken on footrot in sheep, it is necessary that a reliable and repeatable method to categorise the phenotype is available. this paper summarises the methods used and results obtained from 1600 hoof lesion scores of 100 mixed-age ewes independently scored twice by two trained operators. using a 5-pont scale describing the severity of foot lesions, residual correlations were used to assess agreement between scorers and scoring occasions. data were analysed using ... | 2008 | 18478350 |
| pilot trials in australia on eradication of footrot by flock specific vaccination. | footrot is a contagious disease of ruminants requiring strains of dichelobacter nodosus that possess virulence factors including proteases and fimbriae. sheep can be immunised against footrot using vaccine-containing fimbriae, either native or recombinant. the fimbriae are responsible for the serological k-agglutination reaction, which has been used to classify field isolates into nine major serogroups. the range of protection conferred by vaccination is largely restricted to the serogroup invol ... | 2008 | 18621488 |
| proteomic profiling of ovine serum by seldi-tof ms: optimisation, reproducibility and feasibility of biomarker discovery using routinely collected samples. | the diagnosis of infectious diseases in animals may be enhanced by study of the serum proteome in which myriad components are influenced by physiological and pathological processes. surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (seldi-tof ms) has the capacity to detect known and unknown immunologically relevant molecules in the serum proteome. optimum combinations of proteinchip array surfaces, energy absorbing molecules, sample dilutions and instrument settings w ... | 2010 | 18775566 |
| variation in fusobacterium necrophorum strains present on the hooves of footrot infected sheep, goats and cattle. | footrot is a disease of sheep, goats and cattle that causes losses in production and raises welfare issues world-wide. the disease is characterised by destruction of the hard keratin of the hoof leading to lameness, and both dichelobacter nodosus (d. nodosus) and fusobacterium necrophorum (f. necrophorum) are thought to be involved in the etiology of this disease. while a lot is known about the genetic diversity of d. nodosus, very little is known about variation in f. necrophorum, especially as ... | 2009 | 19019570 |
| isolation of the bacteriophage dinohi from dichelobacter nodosus and its interactions with other integrated genetic elements. | the gram-negative anaerobic pathogen dichelobacter nodosus carries several genetic elements that integrate into the chromosome. these include the inta, intb, intc and intd elements, which integrate adjacent to csra and pnpa, two putative global regulators of virulence and the virulence-related locus, vrl, which integrates into ssra. treatment of d. nodosus strains with ultraviolet light resulted in the isolation of dinohi, a member of the siphoviridae and the first bacteriophage to be identified ... | 2008 | 19088904 |
| melatonin enhances the immune response to vaccination against a1 and c strains of dichelobacter nodosus. | melatonin has been shown to exert immunomodulatory properties with broad application in veterinary medicine. here we have investigated the effect of exogenous melatonin in the improvement of the immune response to administration of an immune-preparation of two stumps of a1 and c strains of dichelobacter nodosus in sheep. subcutaneous administration of melatonin enhanced plasma levels of melatonin from days 42 to 120. administration of melatonin to vaccinated animals enhanced both the titer of an ... | 2009 | 19166891 |
| effect of parenteral selenium administration to sheep on prevalence and recovery from footrot. | insufficient intake of selenium (se) is common in many regions, and can contribute to increased susceptibility to and prolonged recovery from infectious diseases. | 2009 | 19192142 |
| melatonin, as an adjuvant-like agent, enhances platelet responsiveness. | melatonin exerts immunomodulatory actions that enhance the magnitude and quality of immune responses specific for certain antigens; this has raised the possibility of using melatonin to design novel vaccine adjuvant systems. the present study investigated the effect of subcutaneous slow-release melatonin implants and subcutaneous melatonin injections on the responsiveness of circulating platelets in sheep after vaccination against dichelobacter nodosus (a1 and c serotypes), the bacterium that ca ... | 2009 | 19196436 |
| dichelobacter nodosus, fusobacterium necrophorum and the epidemiology of footrot. | footrot is a debilitating disease of sheep resulting in lameness, production losses and suffering. to study the basic bacteriology of the disease, a survey was initiated across commercial farms and non-commercial research flocks to compare the bacteriology of symptomatic footrot infected sheep with healthy asymptomatic sheep. of the 80 farmers initially contacted, 14 collected hoof swabs and returned the swabs by post. following dna extraction, species-specific pcr was used to identify if dichel ... | 2009 | 19239925 |
| the detection of dichelobacter nodosus and fusobacterium necrophorum from footrot lesions in new zealand goats. | 2009 | 19248838 | |
| serological diversity and virulence determination of dichelobacter nodosus from footrot in india. | one hundred and twenty-eight swab samples from footrot lesions of naturally infected sheep were examined for presence of dichelobacter nodosus (d. nodosus). the detection of d. nodosus was carried out by polymerase chain reaction (pcr), directly from swabs or after isolation, using 16s rdna specific primers. the isolation of the bacterium was carried out anaerobically on trypticase-arginine-serine (tas) agar containing 4% hoof powder. serogrouping of the d. nodosus was accomplished with multiple ... | 2009 | 19272343 |
| identification of two new dichelobacter nodosus strains in germany. | the multi-strain anaerobe dichelobacter nodosus causes footrot, a contagious disease of the digits of ruminants that occurs worldwide. eleven d. nodosus-like isolates from footrot lesions of sheep in germany were investigated by pcr amplification of the variable region of the d. nodosus fimbrial (fima) gene. in six isolates, fima pcr amplicons of a size suggestive of d. nodosus were generated. single-strand conformational polymorphism (sscp) analysis of the amplicons revealed three banding patte ... | 2010 | 19285440 |
| the intd mobile genetic element from dichelobacter nodosus, the causative agent of ovine footrot, is associated with the benign phenotype. | the gram-negative anaerobic pathogen dichelobacter nodosus is the principal causative agent of footrot in sheep. the inta, intb and intc elements are mobile genetic elements which integrate into two trna genes downstream from csra (formerly glpa) and pnpa in the d. nodosus chromosome. csra homologues act as global repressors of virulence in several bacterial pathogens, as does polynucleotide phosphorylase, the product of pnpa. we have proposed a model in which virulence in d. nodosus is controll ... | 2009 | 19327404 |
| detection of fusobacterium necrophorum and dichelobacter nodosus in lame cattle on dairy farms in new zealand. | lameness in the dairy industry in new zealand causes a problem in lost production, animal welfare and associated costs. to understand what bacteria may be present on the hooves of lame dairy cattle in this grass-fed system, samples were scraped from lame dairy cows and examined for the presence of fusobacterium necrophorum (f. necrophorum) and dichelobacter nodosus (d. nodosus) using the polymerase chain reaction (pcr). the pcr primers were designed to detect the presence of the lkta gene, which ... | 2009 | 19409584 |
| comparative genomic analysis of ten streptococcus pneumoniae temperate bacteriophages. | streptococcus pneumoniae is an important human pathogen that often carries temperate bacteriophages. as part of a program to characterize the genetic makeup of prophages associated with clinical strains and to assess the potential roles that they play in the biology and pathogenesis in their host, we performed comparative genomic analysis of 10 temperate pneumococcal phages. all of the genomes are organized into five major gene clusters: lysogeny, replication, packaging, morphogenesis, and lysis ... | 2009 | 19502408 |
| discrimination between bacteroides, dichelobacter, fusobacterium, porphyromonas and prevotella isolated from caprine footrot by pcr-rflp - short communication. | footrot is widely considered the most severe and most common foot pathology in small ruminants. this study tested the ability of a molecular typing system based on polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) assay of the 16s rrna gene to discriminate between the strict anaerobe genera most commonly isolated from footrot ( bacteroides, dichelobacter, fusobacterium, porphyromonas and prevotella ) in goats in extremadura (spain), with a view to facilitating identif ... | 2009 | 19584033 |
| evolution of oxidative/nitrosative stress biomarkers during an open-field vaccination procedure in sheep: effect of melatonin. | melatonin has been shown to exert immunomodularory properties with broad application in veterinary medicine. in previous work we have described that subcutaneous coadministration of melatonin to seeps vaccinated against two stumps of a1 and c strains of dichelobacter nodosus enhanced both the antibody titer and serum igg levels to a1 and c strains of d. nodosus compared to vaccinated animals not treated with melatonin. following a similar protocol here we have investigated the effect of a higher ... | 2010 | 19631992 |
| modulation of inter-vaccination interval to avoid antigenic competition in multivalent footrot (dichelobacter nodosus) vaccines in sheep. | virulent footrot is a significant disease of sheep in most sheep farming countries; a strain/serogroup of the anaerobic bacterium dichelobacter nodosus is the essential transmitting agent. commercial multivalent footrot vaccines containing nine fimbrial serogroups (a through i) of d. nodosus produce relatively low and short term antibody responses due to antigenic competition, in contrast to higher and longer responses provided by monovalent or bivalent vaccines. the latter were important compon ... | 2009 | 19853681 |
| deletion of the c-terminus of polynucleotide phosphorylase increases twitching motility, a virulence characteristic of the anaerobic bacterial pathogen dichelobacter nodosus. | the gram-negative anaerobe dichelobacter nodosus is the causative agent of footrot in sheep. different strains of d. nodosus cause disease of differing severities, ranging from benign to virulent. virulent strains have greater twitching motility and secrete proteases that are more thermostable than those secreted by benign strains. we have identified polynucleotide phosphorylase (pnpase) as a putative virulence regulator and have proposed that pnpase expression is modulated by the adjacent integ ... | 2010 | 19895640 |
| [structural and functional specificity of the dichelobacter nodosus pili. synthesis of the protein pilin of d. nodosus in various bacterial recombinant strains]. | the ovine foot rot is a severe infectious disease of sheep. dichelobacter nodosus is an essential pathogen of this disease. an obligatory anaerobic gram-negative rod-shaped microorganism has slow rate of accumulating bacterial density and fastidious growth requirements. this causes obstacles to vaccine production and makes it difficult to diagnose the disease. the diagnosis in this case is more expensive. fimbriae (or pili) are one of the major factors of virulence of d. nodosus. their antigenic ... | 2009 | 20050159 |
| understanding the molecular epidemiology of the footrot pathogen dichelobacter nodosus to support control and eradication programs. | the gram-negative anaerobe dichelobacter nodosus is the primary etiologic agent of ovine footrot. few studies of the genetic diversity and epidemiology of d. nodosus have been done, despite the economic cost and welfare implications of the disease. this study examined a large collection of australian isolates; 735 isolates from footrot-infected sheep from 247 farms in western australia (wa) were tested by pulsed-field gel electrophoresis (pfge), and a subset of 616 isolates was tested by infrequ ... | 2010 | 20071558 |
| crystallization of the virulent and benign subtilisin-like proteases from the ovine footrot pathogen dichelobacter nodosus. | dichelobacter nodosus is the principal causative agent of ovine footrot, a disease of significant economic importance to the sheep industry. d. nodosus secretes a number of subtilisin-like serine proteases which mediate tissue damage and presumably contribute to the pathogenesis of footrot. strains causing virulent footrot secrete the proteases aprv2, aprv5 and bprv and strains causing benign footrot secrete the closely related proteases aprb2, aprb5 and bprb. here, the cloning, purification and ... | 2010 | 20208163 |
| diagnostic sampling strategies for virulent ovine footrot: simulating detection of dichelobacter nodosus serogroups for bivalent vaccine formulation. | dichelobacter nodosus is a slow-growing anaerobic bacterium that is the causative agent of virulent ovine footrot. vaccination targeted at up to two specific serogroups can eliminate those serogroups from infected flocks, but requires identification of serogroups present in infected flocks. serogroups can be identified using slide agglutination or polymerase chain reaction (pcr) methods. the objectives of this project were to use stochastic simulation modeling to estimate the efficacy of samplin ... | 2010 | 20303191 |
| footrot and interdigital dermatitis in sheep: farmer satisfaction with current management, their ideal management and sources used to adopt new strategies. | the aims of this research were to identify management practices that sheep farmers currently use to treat and prevent footrot in sheep and whether they consider that these are successful management tools and to find out how sheep farmers would ideally like to manage footrot in their flock. over 90% of lameness in sheep in the uk is caused by dichelobacter nodosus, which presents clinically as interdigital dermatitis (id) alone or with separation of hoof horn (fr). a questionnaire was sent to 265 ... | 2010 | 20594602 |
| a within farm clinical trial to compare two treatments (parenteral antibacterials and hoof trimming) for sheep lame with footrot. | from observational studies, farmers who use parenteral antibacterials to promptly treat all sheep with footrot (fr) or interdigital dermatitis (id) have a prevalence of lameness of < 2% compared with a prevalence of 9% lameness reported by farmers who treat lame sheep by trimming affected feet. we tested the hypothesis that prompt treatment of sheep lame with naturally developing fr or id with parenteral and topical antibacterials reduces the prevalence and incidence of lameness with these condi ... | 2010 | 20627343 |
| detection and diversity of a putative novel heterogeneous polymorphic proline-glycine repeat (pgr) protein in the footrot pathogen dichelobacter nodosus. | dichelobacter nodosus, a gram-negative anaerobic bacterium, is the essential causative agent of footrot in sheep. currently, depending on the clinical presentation in the field, footrot is described as benign or virulent; d. nodosus strains have also been classified as benign or virulent, but this designation is not always consistent with clinical disease. the aim of this study was to determine the diversity of the pgr gene, which encodes a putative proline-glycine repeat protein (pgr). the pgr ... | 2011 | 20655152 |
| ovine footrot: new approaches to an old disease. | footrot is a bacterial disease that has substantial economic and welfare impacts in sheep and can be difficult to manage. research is focussed on reducing the impact that footrot has on farmers and their flocks and better understanding the aetiology of the disease. key areas of current research include, developing better vaccines, deploying tailored vaccines in a specific and targeted fashion on individual farms, analysing and developing better farm management practices to suit specific sheep fa ... | 2010 | 20926208 |
| the subtilisin-like protease aprv2 is required for virulence and uses a novel disulphide-tethered exosite to bind substrates. | many bacterial pathogens produce extracellular proteases that degrade the extracellular matrix of the host and therefore are involved in disease pathogenesis. dichelobacter nodosus is the causative agent of ovine footrot, a highly contagious disease that is characterized by the separation of the hoof from the underlying tissue. d. nodosus secretes three subtilisin-like proteases whose analysis forms the basis of diagnostic tests that differentiate between virulent and benign strains and have bee ... | 2010 | 21124876 |
| footrot on a sheep breeding farm in the himalayan state of jammu and kashmir. | in the present study ovine footrot was detected clinically on a sheep farm in the himalayan state of jammu and kashmir. dichelobacter nodosus was confirmed by culture and polymerase chain reaction (pcr) using species-specific 16s ribosomal rna primers. when cultured, the organism appeared as flat colourless colonies having a fine granulated structure with irregular margins, and showing characteristic gram-negative rods with swollen ends. detection by pcr from cultured bacteria resulted in amplif ... | 2010 | 21309465 |
| disease resistance in merino sheep. iii. genetic variation in resistance to footrot following challenge and subsequent vaccination with an homologous rdna pilus vaccine under both induced and natural conditions. | summary: eight traits representing clinical indicators of resistance to footrot were examined in 1562 merino sheep, representing the progeny from 162 sires in four major bloodlines. over a 4-year period, sheep were exposed to virulent isolates of dicbelobacter nodosus under both an experimental challenge in which footrot was induced, and a separate natural challenge involving a different isolate of d. nodosus. five footrot traits and three healing traits were each recorded on seven occasions fol ... | 1994 | 21395787 |
| determination of prevalence and economic impact of ovine footrot in central kashmir india with isolation and molecular characterization of dichelobacter nodosus. | the present study determines the prevalence, economic impact of virulent footrot in central kashmir, india, along with isolation and molecular characterization of dichelobacter nodosus (d. nodosus) where so far no such work has been carried out. over all 12.54% prevalence of footrot was recorded in central kashmir with highest (15.84%) in district srinagar, and least (10.89%) in district budgam, while it was 13.28% in district ganderbal. overall economic impact of footrot was estimated to the tu ... | 2011 | 21397709 |
| ovine pedomics: the first study of the ovine foot 16s rrna-based microbiome. | we report the first study of the bacterial microbiome of ovine interdigital skin based on 16s rrna by pyrosequencing and conventional cloning with sanger-sequencing. three flocks were selected, one a flock with no signs of footrot or interdigital dermatitis, a second flock with interdigital dermatitis alone and a third flock with both interdigital dermatitis and footrot. the sheep were classified as having either healthy interdigital skin (h) and interdigital dermatitis (id) or virulent footrot ... | 2011 | 21430786 |
| prevalence of footrot in swedish slaughter lambs. | abstract: | 2011 | 21492433 |
| implications of host genetic variation on the risk and prevalence of infectious diseases transmitted through the environment. | previous studies have shown that host genetic heterogeneity in the response to infectious challenge can affect the emergence risk and the severity of diseases transmitted through direct contact between individuals. however, there is substantial uncertainty about the degree and direction of influence owing to different definitions of genetic variation, most of which are not in line with the current understanding of the genetic architecture of disease traits. also, the relevance of previous result ... | 2011 | 21527777 |
| the pathogenesis of ovine footrot. | ovine footrot is a contagious and debilitating disease that is of major economic significance to the sheep meat and wool industries. the causative bacterium is the gram negative anaerobe dichelobacter nodosus. research that has used a classical molecular genetics approach has led to major advances in our understanding of the role of the key virulence factors of d. nodosus in the disease process. d. nodosus strains produce polar type iv fimbriae and extracellular serine proteases. mutagenesis of ... | 2011 | 21596496 |
| s1 pocket of a bacterially derived subtilisin-like protease underpins effective tissue destruction. | the ovine footrot pathogen, dichelobacter nodosus, secretes three subtilisin-like proteases that play an important role in the pathogenesis of footrot through their ability to mediate tissue destruction. virulent and benign strains of d. nodosus secrete the basic proteases bprv and bprb, respectively, with the catalytic domain of these enzymes having 96% sequence identity. at present, it is not known how sequence variation between these two putative virulence factors influences their respective ... | 2011 | 21990366 |
| plasmid-mediated florfenicol resistance in mannheimia haemolytica isolated from cattle. | the aim of this study was to analyse a florfenicol-resistant mannheimia haemolytica isolated from a calf to determine the genetic basis of its florfenicol-resistance. the antimicrobial susceptibility and plasmid content of the isolate were determined. a florfenicol resistant plasmid carrying the flor gene was identified by pcr and transformed into escherichia coli jm109 and hb101 strains. the plasmid was then mapped and sequenced completely. the isolate was resistant to chloramphenicol, florfeni ... | 2011 | 22019290 |
| ultrahigh resolution and full-length pilin structures with insights for filament assembly, pathogenic functions, and vaccine potential. | pilin proteins assemble into type iv pili (t4p), surface-displayed bacterial filaments with virulence functions including motility, attachment, transformation, immune escape, and colony formation. however, challenges in crystallizing full-length fiber-forming and membrane protein pilins leave unanswered questions regarding pilin structures, assembly, functions, and vaccine potential. here we report pilin structures of full-length dnfima from the sheep pathogen dichelobacter nodosus and ftpile fr ... | 2011 | 22027840 |
| Identification of two new serotypes within serogroup B of Dichelobacter nodosus. | The present study records the strain-specific molecular typing system for Dichelobacter nodosus (D. nodosus) based on genetic analysis of fimA locus. Based on the study two new serotypes B5 and B6 are reported within the serogroup B. Out of 200 swab samples collected randomly from foot lesions of footrot affected sheep from all the districts of Kashmir, India, 122 (61.0%) detected positive for D. nodosus. Serogroup B was predominantly prevalent in 83.60% of positive samples. Restriction fragment ... | 2011 | 22197966 |
| impact of footrot vaccination and antibiotic therapy on footrot and contagious ovine digital dermatitis. | footrot and contagious ovine digital dermatitis (codd) are common causes of foot disease of sheep in the uk. the study reported here is a split flock randomised treatment trial undertaken on a group of 748 fattening lambs on a uk sheep farm affected by codd and footrot. the sheep were randomly assigned to one of two treatment protocols. in protocol a, all sheep were given two doses of footrot vaccine (footvax, msd), plus targeted antibiotic therapy (long-acting amoxicillin, betamox la, norbrook ... | 2012 | 22266683 |
| development and comparison of a real-time pcr assay for detection of dichelobacter nodosus with culturing and conventional pcr: harmonisation between three laboratories. | ovine footrot is a contagious disease with worldwide occurrence in sheep. the main causative agent is the fastidious bacterium dichelobacter nodosus. in scandinavia, footrot was first diagnosed in sweden in 2004 and later also in norway and denmark. clinical examination of sheep feet is fundamental to diagnosis of footrot, but d. nodosus should also be detected to confirm the diagnosis. pcr-based detection using conventional pcr has been used at our institutes, but the method was laborious and t ... | 2012 | 22293440 |
| possible cross-infection of dichelobacter nodosus between co-grazing sheep and cattle. | the aim of this study was to investigate possible cross-infection of dichelobacter nodosus in norwegian farms practising co-grazing of sheep and cattle. | 2012 | 22458248 |
| bovine digital dermatitis: possible pathogenic consortium consisting of dichelobacter nodosus and multiple treponema species. | bovine digital dermatitis (dd) is a multifactorial disease involving at least one or more treponemal species. virulent phylotypes of treponema and other infectious agents contributing to disease etiology still remain to be identified. this study addressed these questions by analyzing the prevalence and distribution of seventeen phylotypes of treponema in dd lesions by fluorescent in situ hybridization (fish) applying species/phylotype-specific oligonucleotide probes. in situ hybridization for di ... | 2012 | 22698300 |
| fusobacterium necrophorum, and not dichelobacter nodosus, is associated with equine hoof thrush. | the aim of this study was to determine which of the two species, fusobacterium necrophorum or dichelobacter nodosus, are associated with hoof thrush in horses. fourteen hoof samples, collected from eight horses with thrush and 14 samples collected from eight horses with healthy hooves, were examined for the presence of f. necrophorum, fusobacterium equinum and d. nodosus. only isolates with phenotypic characteristics representing fusobacterium could be cultured. total dna extracted from the 28 h ... | 2013 | 22909990 |
| infection dynamics of digital dermatitis in first-lactation holstein cows in an infected herd. | digital dermatitis (dd) refers to painful lesions primarily affecting the skin in the interdigital region of dairy cattle. the purpose of this study was to evaluate the dynamics of dd in 39 cows, observed at approximately 3-d intervals, for the first 6 mo of lactation. specifically, the study aimed at evaluating different levels of dd susceptibility in cows, identifying the bacterial colonization of the interdigital skin, and exploring the relationship between clinical dd diagnosis and laborator ... | 2012 | 22939796 |
| comparative study of the commonly used virulence tests for laboratory diagnosis of ovine footrot caused by dichelobacter nodosus in australia. | footrot in sheep and goats is expressed as a spectrum of clinical entities ranging from benign, which is a self limiting interdigital dermatitis to highly virulent, in which severe under running of the horn of the hoof occurs. interactions between the host, the virulence of the causative strain of dichelobacter nodosus and environmental conditions determine the severity of the disease. clinical diagnosis of virulent footrot, which a notifiable disease in some states of australia, is not always s ... | 2013 | 23084506 |
| the aprv5 subtilase is required for the optimal processing of all three extracellular serine proteases from dichelobacter nodosus. | dichelobacter nodosus is the principal causative agent of ovine footrot and its extracellular proteases are major virulence factors. virulent isolates of d. nodosus secrete three subtilisin-like serine proteases: aprv2, aprv5 and bprv. these enzymes are each synthesized as precursor molecules that include a signal (pre-) peptide, a pro-peptide and a c-terminal extension, which are processed to produce the mature active forms. the function of the c-terminal regions of these proteases and the mech ... | 2012 | 23112874 |
| characterisation of dichelobacter nodosus isolates from norway. | an outbreak of ovine footrot in norway in 2008, the first reported since 1948, prompted action to investigate norwegian isolates of dichelobacter nodosus. a total of 579 isolates from 124 different farms were characterised. these included 519 isolates from sheep, 52 isolates from cattle and 8 isolates from goats. the potential virulence of the isolates was assessed by the gelatin gel test (gg-test) and the elastin agar test, that test the heat stability and elastase activity of bacterial proteas ... | 2013 | 23332560 |
| an evaluation of the ability of dichelobacter nodosus to survive in soil. | dichelobacter nodosus is the causative agent of footrot in sheep. the survival of the bacterium in soil is of importance for the epidemiology of the disease. the investigation evaluates the survival of d. nodosus in soil with and without added hoof powder stored under different temperatures. | 2013 | 23343097 |