Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| production of branched-chain volatile fatty acids by certain anaerobic bacteria. | net production of isobutyric acid, isovaleric acid, and 2-methylbutyric acid by cultures of bacteroides ruminicola and megasphaera elsdenii on media that contained trypticase or casein hydrolysate continued (up to 5 days) after growth had ceased. only trace quantities of these acids were produced in a medium that contained a mixture of amino acids that did not include the branched-chain amino acids. m. elsdenii produced increased quantities of the branched-chain fatty acids in a medium that cont ... | 1978 | 566082 |
| amino acid uptake systems in bacteroides ruminicola. | uptake of amino acids by bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. a high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. all amino acids except proline were taken up and incorporated into cold trichloroacetic acid precipitable material. different patterns of incorporation and different responses to 2,4-dinitrophenol and potassium fer ... | 1979 | 575310 |
| origins of fermentation products formed during growth of bacteroides ruminicola on glucose. | bacteriodes ruminicola grown on complex medium with glucose as carbon source gave acetate, co2, formate and succinate as main fermentation products. no evidence was found for significant glucose catabolism by pathways other than the embden-meyerhof sequence. however, [u-14c]glucose fermentation gave products whose specific radioactivities were much lower than expected. there appear to be two main causes. firstly, a rapid exchange occurred between metabolic intermediates and co2, probably due to ... | 1978 | 670931 |
| inhibitory effects of h2 on growth of clostridium cellobioparum. | hydrogen inhibits the growth of hydrogen-producing clostridium cellobioparum, but not of escherichia coli or bacteroides ruminicola. the inhibition is reversible. when hydrogen was removed either by palladium black or by gassing out the tube, glucose utilization increased as did optical density and hydrogen production of c. cellobioparum. removal of the h2 by methanogenic bacteria favors the growth of c. cellobioparum. grown with methanobacterium ruminantium in various concentrations of glucose, ... | 1976 | 779644 |
| characterization of the predominant bacteria occurring in the rumen of goats (capra hircus). | a total of 44 strains of bacteria were isolated from rumen contents of the goat. based on morphology, gram stain, anaerobiosis, motility, and fermentation end products, they were grouped into 11 different types. for each type, all or representative strains were characterized in detail. the type, number of strains characterized over total number of strains, and identification were as follows: type 1, 6/21, atypical butyrivibrio fibriosolvens; type 2, 6/9, atypical butyrivibrio fibrisolvens; type ... | 1977 | 879766 |
| urease assay and urease-producing species of anaerobes in the bovine rumen and human feces. | a growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that peptostreptococcus productus is often the most numerous urease-forming species in human feces. also, some fecal strains of ruminococcus albus, clostridium innocuum, and clostridium beijerinckii produced urease. single strains of fusobacterium prausnitzii, coprococcus catus, and streptococcus mitis th ... | 1977 | 879770 |
| tetrapyrrole utilization by bacteroids ruminocola. | reduced versus oxidized difference spectra of whole cells and pyridine hemochromogens of heme-requiring isolates of bacteroides ruminicola are altered when deuteroporphyrin or mesoporphyrin replaces protoheme as a growth factor. during growth in the presence of either deuteroporphyrin or mesoporphyrin, whole cells exhibit peaks at 545 t547, 515 to 518, and 412 to 413 nm. pyridine hemochromogen spectra confirm the presence of meso -or deuteroheme in cells grown in the presence of meso- or deutero ... | 1977 | 893342 |
| [the bacteriodaceae flora in the faeces of pigs (author's transl)[]. | a total of 180 bacteroidaceae strains from the fecal specimens of 31 clinically healthy pigs aged 120 to 180 days from 4 farms were isolated in numbers of 10(8)-10(10) per gram feces and differentiated for their morphological, cultural and biochemical properties. 161 of these strains belonged to bacteroides ruminicola and related species, 10 to b. fragilis ss. fragilis, 3 to b. fragilis ss. vulgatus, 4 to fusobacterium necrogenes, 1 to f. mortiferum and 1 to a non-motile variant of f. novum. the ... | 1976 | 936825 |
| metabolism and growth yields in bacteroides ruminicola strain b14. | metabolism of d-glucose by bacteroides ruminicola subsp. brevis, strain b14, has been examined. growth yield studies gave molar growth yields, corrected for storage polysaccharide, of approximately 66 g (dry weight)/mol of glucose fermented. the storage polysaccharide amounted to about 14% of the total dry weight, or 55% of the total cellular carbohydrate, at full growth. after correcting glucose utilization for incorporation into cellular carbohydrate, measurement of product formation showed th ... | 1976 | 970946 |
| when death necessitates selling a practice. | 2005 | 1070377 | |
| [additional methods of examination in tumors and cysts of the mediastinum]. | 2004 | 1133952 | |
| effect of actinomycin d and oxygen on the ribonucleic acid synthesis of an anaerobic gram-negative bacterium. | the synthesis of ribonucleic acid by whole cells of bacteroides ruminicola is not sensitive to actinomycin d, but it is sensitive to actinomycin d in the presence of ethylenediaminetetraacetate. ribonucleic acid synthesis by whole cells of this gram-negative anaerobic bacterium is also totally inhibited by oxygen. | 1975 | 1147598 |
| proceedings: systems connecting endoplasmic reticulum with axon and dendrites. | 2005 | 1151806 | |
| fermentation products in the rumen of a gnotobiotic lamb dosed with bacteroides ruminicola. | 1975 | 1187635 | |
| a 2-year follow-up of 72 hyperactive boys. classroom behavior and peer acceptance. | a 2-year, prospective follow-up of 72 hyperactive boys (94 per cent of the initial sample) examined classroom and home behavior, academic achievement, peer status, and depressive symptomatology for patients and a matched control group. sixty-five per cent of the sample was still on medication at follow-up. the patient group continued to manifest behavioral and academic difficulty. off-drug classroom behavior showed considerable stability from baseline to 2-year follow-up which did not appear to ... | 2005 | 1249566 |
| epidemiologic observations on suicides in virginia. | 2005 | 1266370 | |
| glucose toxicity and inability of bacteroides ruminicola to regulate glucose transport and utilization. | ammonia-limited (3.5 mm ammonia) cultures of bacteroides ruminicola b(1)4 had a high number of viable cells (greater than 10(9)/ml), but only when the concentration of glucose was not too high (10 mm or less). when the glucose concentration was increased from 10 to 50 mm, there was a marked decrease in viability (10(5)-fold or greater). because there was little decline in ph and only a small increase in succinate and acetate as the glucose concentration was increased, it did not appear that end ... | 1992 | 1320369 |
| presynaptic dopaminergic inhibition of the spinal reflex in rats. | dopaminergic influence on spinal monosynaptic transmission was examined in rats. monosynaptic mass reflex (mmr) was recorded from the ventral root l6 following supramaximal stimulation (0.2 hz; 0.1 ms) to the ipsilateral dorsal root l6 in spinalized rat under pentobarbitone sodium (40 mg/kg, i.p.) anaesthesia. mmr was inhibited by intravenous administration of the dopaminergic agonist, apomorphine (50-200 ug/kg) in a dose-dependent manner. the attenuatory effect of apomorphine (200 ug/kg i.v.) o ... | 1992 | 1352179 |
| deoxyribonuclease activity in rumen bacteria. | deoxyribonuclease activity was surveyed in 22 strains belonging to 12 species of rumen bacteria, with lambda bacteriophage dna as substrate. activity was readily detected in broken cell preparations from 15 of these strains. particularly high levels of activity were present in cells and culture supernatant of all 5 strains of bacteroides succinogenes, and 2 out of 6 strains of bacteroides ruminicola, examined. | 1990 | 1366671 |
| [gastric emptying of solids, acid secretion and tobacco in duodenal ulcer]. | we study in a group of patients with endoscopically diagnosed duodenal ulcer (19; 17 males) and controls (11; 7 males) the gastric emptying of solids through scintigraphy and gastric acid secretion by standard tests. in the same way we investigated prospectively some clinical data, specially smoking habits. as a whole, patients with duodenal ulcer showed an emptying of solids slightly faster than controls (t 1/2-minutes-: 85.4 +/- 28.6 in patients with duodenal ulcer versus 116.9 +/- 46.5 in con ... | 1992 | 1389553 |
| [the characteristics and regulation of glutamate dehydrogenase in strains of bacteroides ruminicola ssp. ruminicola isolated from the rumen of fallow deer]. | very little information about nh4+ assimilation paths in rumen anaerobic bacteria is available, and the information about wild animals is completely missing. glutamate dehydrogenase (gdh) isolated from the rumen strain b. ruminicola in fallow deer was purified and its properties were specified after crystalline ammonium sulphate precipitation and gel filtration on sephadex g-200. the properties of partly purified gdh were specified. one of the first specifications concerning gdh from various sou ... | 1992 | 1413388 |
| properties of a genetically reconstructed prevotella ruminicola endoglucanase. | a puc19-derived plasmid was constructed that coded for a hybrid cellulase with the thermomonospora fusca e2 cellulose-binding domain at its c terminus joined to the prevotella ruminicola 40.5-kda carboxymethyl cellulase (cmcase). the hybrid enzyme was purified and characterized enzymatically. it bound tightly to cellulose, and its specific activities on carboxymethyl cellulose, amorphous cellulose, and ball-milled cellulose were 1.5, 10, and 8 times that of the 40.5-kda cmcase, respectively. fur ... | 1992 | 1482181 |
| distribution of xylanase genes and enzymes among strains of prevotella (bacteroides) ruminicola from the rumen. | the distribution of two xylanase genes was examined by southern hybridization among 26 strains of the rumen anaerobic bacterium prevotella (bacteroides) ruminicola. hybridization with a xylanase/endoglucanase gene from the type strain 23 was found in six strains while hybridization with a xylanase gene from strain d31d was found in 14 strains. sequences related to both genes were present, on different restriction fragments, in six strains, whereas no hybridization to either gene was detected in ... | 1992 | 1490595 |
| selective isolation of bacteria with dipeptidyl aminopeptidase type i activity from the sheep rumen. | five-hundred-and-six fresh isolates of rumen bacteria were tested for their ability to hydrolyse the synthetic substrate for dipeptidyl aminopeptidase type i, glyarg-4-methoxy-2-naphthylamide (glyarg-mna), using a gel overlay technique. twelve positive isolates were small gram-negative rods which resembled bacteroides ruminicola in their biochemical and morphological properties. sds-page of whole cell extracts indicated that two were similar to b. ruminicola strain b14, six resembled b. ruminico ... | 1992 | 1526449 |
| interaction of ruminal bacteria in the production and utilization of maltooligosaccharides from starch. | the degradation and utilization of starch by three amylolytic and one nonamylolytic species of ruminal bacteria were studied. pure cultures of streptococcus bovis jb1, butyrivibrio fibrisolvens 49, and bacteroides ruminicola d31d rapidly hydrolyzed starch and maltooligosaccharides accumulated. the major starch hydrolytic products detected in s. bovis cultures were glucose, maltose, maltotriose, and maltotetraose. in addition to these oligosaccharides, b. fibrisolvens cultures produced maltopenta ... | 1992 | 1539992 |
| properties of ionophore-resistant bacteroides ruminicola enriched by cultivation in the presence of tetronasin. | bacteroides ruminicola m384 was grown in the presence of increasing concentrations of tetronasin, an ionophore that has been developed as a feed additive for ruminants. the resulting culture, b. ruminicola m384/tnr, was then maintained in medium containing 0.1 microgram tetronasin/ml. growth of the parent strain was eliminated by the addition of 0.1 micrograms tetronasin/ml, but the growth rate of b. ruminicola m384/tnr, which grew more slowly than the parent strain, was unaffected by adding tet ... | 1992 | 1541602 |
| evidence for natural transfer of a tetracycline resistance gene between bacteria from the human colon and bacteria from the bovine rumen. | previously, we demonstrated conjugal transfer of a specially constructed shuttle vector, prdb5, from the human colonic anaerobe bacteroides uniformis to the ruminal anaerobe prevotella (bacteroides) ruminicola b(1)4. we have now shown that naturally occurring gene transfer elements in bacteroides species and prevotella ruminicola can also be transferred between these two genera. a self-transmissible chromosomal element originally found in a clinical isolate of bacteroides fragilis (tcr emr 12256 ... | 1992 | 1599250 |
| dna sequence and transcription of an endoglucanase gene from prevotella (bacteroides) ruminicola ar20. | the endoglucanase gene was sequenced from prevotella ruminicola ar20, isolated as clone pjw4. the endoglucanase (brend) is encoded by an open reading frame (orf1) of 501 codons, corresponding to a protein of calculated molecular weight 55.7 kda. analysis of proteins on sds-page revealed a protein corresponding to the calculated molecular weight of the processed brend. the protein showed substantial homology to members of the a4 sub-family cellulases. primer extension studies revealed that transc ... | 1992 | 1603069 |
| some growth and metabolic characteristics of monensin-sensitive and monensin-resistant strains of prevotella (bacteroides) ruminicola. | new strains with enhanced resistance to monensin were developed from prevotella (bacteroides) ruminicola subsp. ruminicola 23 and p. ruminicola subsp. brevis ga33 by stepwise exposure to increasing concentrations of monensin. the resulting resistant strains (23mr2 and ga33mr) could initiate growth in concentrations of monensin which were 4 to 40 times greater than those which inhibited the parental strains. resistant strains also showed enhanced resistance to nigericin and combinations of monens ... | 1992 | 1622231 |
| vitamin b12-dependent propionate production by the ruminal bacterium prevotella ruminicola 23. | when prevotella ruminicola 23 was grown in a defined medium containing a vitamin mixture, significant amounts of propionate were formed. succinate and acetate were the major fermentation acids produced when vitamins were omitted, and further experiments demonstrated that propionate formation was dependent on vitamin b12. when the organism was grown in continuous culture at dilution rates of less than 0.20 h-1, propionate and acetate were the predominant fermentation products and little succinate ... | 1992 | 1637169 |
| enzymes associated with metabolism of xylose and other pentoses by prevotella (bacteroides) ruminicola strains, selenomonas ruminantium d, and fibrobacter succinogenes s85. | prevotella (bacteroides) ruminicola strains b(1)4 and s23 and selenomonas ruminantium strain d used xylose as the sole source of carbohydrate for growth, whereas fibrobacter succinogenes was unable to metabolize xylose. prevotella ruminicola strain b(1)4 exhibited transport activity for xylose. in contrast, f. succinogenes lacked typical xylose uptake activity but did exhibit low binding potential for the sugar. prevotella ruminicola strains b(1)4 and s23 as well as s. ruminantium d showed low x ... | 1992 | 1643581 |
| ion chromatographic methods for the detection of starch hydrolysis products in ruminal digesta. | dionex high-performance ion chromatographic methods were evaluated for separation and quantitation of plant sugars and starch digestion products in the ruminal digesta of cattle. mono- and disaccharides were eluted from a dionex carbopac pa1 column with sodium hydroxide used isocratically or as a ph gradient. maltooligosaccharides which had a degree of polymerization (dp) less than 30 glucose residues were eluted in 60 min by a sodium hydroxide eluent containing a sodium acetate gradient. carboh ... | 1991 | 1653257 |
| utilization of nucleic acids by selenomonas ruminantium and other ruminal bacteria. | species of ruminal bacteria were screened for the ability to grow in media containing rna or dna as the energy source. bacteroides ruminicola d31d and selenomonas ruminantium hd4, ga192, and d effectively used rna for growth, but not dna. b. ruminicola d31d was able grow on nucleosides but not on bases or ribose. the s. ruminantium strains were able to grow when provided with either nucleosides or ribose but not bases. strains of s. ruminantium, but not b. ruminicola d31d, were also able to use ... | 1990 | 1707252 |
| taxonomic relationships among strains of the anaerobic bacterium bacteroides ruminicola determined by dna and extracellular polysaccharide analysis. | dna and extracellular polysaccharide (eps) analyses were performed on 14 strains of bacteroides ruminicola. the guanine-plus-cytosine (g+c) base contents, determined from the buoyant densities of chromosomal dnas, showed a broad range of values, from 37.6 to 50.9 mol%. dna hybridization showed generally low dna relatedness among the strains. seven strains formed two groups of closely related bacteria consisting of five (group 1) and two (group 2) strains, and another strain, e42g, showed moderat ... | 1991 | 1746957 |
| a survey of peptidase activity in rumen bacteria. | twenty-nine strains of 14 species of rumen bacteria were screened for their ability to hydrolyse ala2, ala5, glyarg-4-methoxy-2-naphthylamide (glyarg-mna) and leu-mna. several species, notably megasphaera elsdenii, were active against ala2, and a smaller number, including bacteroides ruminicola, butyrivibrio fibrisolvens, ruminococcus flavefaciens, lachnospira multipara and ruminobacter amylophilus, broke down ala5. streptococcus bovis had an exceptionally high leucine arylamidase activity. howe ... | 1991 | 1748877 |
| conjugal transfer of a shuttle vector from the human colonic anaerobe bacteroides uniformis to the ruminal anaerobe prevotella (bacteroides) ruminicola b(1)4. | prevotella ruminicola (formerly bacteroides ruminicola) is an anaerobic, gram-negative, polysaccharide-degrading bacterium which is found in the rumina of cattle. since p. ruminicola is thought to make an important contribution to digestion of plant material in rumina, the ability to alter this strain genetically might help improve the efficiency of rumen fermentation. however, previously there has been no way to introduce foreign dna into p. ruminicola strains. in this study we transferred a sh ... | 1991 | 1768083 |
| the anti-tachycardic mechanism of a direct-acting vasodilator, budralazine, in rats. | the present study was undertaken to elucidate the anti-tachycardic effect of a direct-acting vasodilator, budralazine, using an electrophysiological technique. normotensive male wistar rats were used. rats were anesthetized intraperitoneally with urethane and alpha-chloralose. intravenous administration of budralazine (0.5-5.0 mg/kg) produced a dose-dependent reduction of mean arterial pressure in anesthetized rats. at doses of 0.5 and 1.0 mg/kg, budralazine induced bradycardia accompanied with ... | 1991 | 1773498 |
| intracellular ph of acid-tolerant ruminal bacteria. | acid-tolerant ruminal bacteria (bacteroides ruminicola b1(4), selenomonas ruminantium hd4, streptococcus bovis jb1, megasphaera elsdenii b159, and strain f) allowed their intracellular ph to decline as a function of extracellular ph and did not generate a large ph gradient across the cell membrane until the extracellular ph was low (less than 5.2). this decline in intracellular ph prevented an accumulation of volatile fatty acid anions inside the cells. | 1991 | 1781695 |
| growth arrest induced by transforming growth factor beta 1 is accompanied by protein phosphatase activation in human keratinocytes. | protein phosphorylation and dephosphorylation are involved in regulation of cell growth. we tested the hypothesis that the growth inhibitory effect of transforming growth factor beta 1 (tgf-beta 1) involves activation of protein phosphatases. exposure of human keratinocytes in culture to 400 pm tgf-beta 1 for 48 h led to 80% inhibition of dna synthesis as measured by nuclear labeling. incubation of cultured keratinocytes with 400 pm tgf-beta 1 rapidly activated (within 30 min) protein serine/thr ... | 1991 | 1847373 |
| pathologic review of consecutive radical prostatectomy specimens. nerve sparing versus nonnerve sparing. | retrospective pathologic analysis was conducted of specimens from 88 radical retropubic prostatectomy operations performed between 1982 and 1987 inclusive. the median age of patients was sixty years (range, 46 to 73 years). of the 88 radical prostatectomies performed, 51 were nerve-sparing (40 bilateral) and 37 were nonnerve-sparing (11 unilateral) procedures. preoperative clinical staging was similar in both groups. thirty-five of 37 patients (95%) in nonnerve-staging group and 51 of 51 patient ... | 2007 | 1887533 |
| investigation and treatment of a multiple limb birth. | we report a baby born with three fully developed normally functioning legs. the third leg was attached to the baby's sacrum via an iliac bone at the site of a spina bifida. a primitive perineum was present. innervation of the limb was via a sacral hiatus with a myelomeningocele. a rudimentary peritoneal sac was found at operation containing a blind loop of bowel suspended by a mesentery. the additional leg was found to have no cortical representation using somatic evoked potentials although lumb ... | 2007 | 1892571 |
| a bacteroides ruminicola 1,4-beta-d-endoglucanase is encoded in two reading frames. | escherichia coli transformed with a plasmid containing a bacteroides ruminicola endoglucanase (carboxymethyl cellulase [cmcase]) gene produced three immunologically cross-reacting cmcases which had molecular weights of 40,500, 84,000, and 88,000, while b. ruminicola produced cmcases with molecular weights of 82,000 and 88,000. the two b. ruminicola enzymes (purified from culture supernatants) had different n-terminal amino acid sequences, but each enzyme was encoded by the same gene (three indep ... | 1991 | 1938895 |
| a new manual method for assessing two-dimensional cancellous bone structure: comparison between iliac crest and lumbar vertebra. | we have developed a simple method for the direct analysis of two-dimensional trabecular structure. using this method, we have compared the structure of the left and right iliac crest and the second lumbar vertebra and studied the relationship between two-dimensional trabecular structure and the ultimate compressive stress of bone samples from the second lumbar vertebra. transverse cores were taken from the left and right iliac crests and second lumbar vertebrae of 35 subjects (12 females) who di ... | 2007 | 1950674 |
| whole-body gallium 67 scans. role in diagnosis of sarcoidosis. | early studies of 67gallium (67ga) scanning in sarcoidosis focused on the lungs as a measure of disease activity, likelihood of progression, and the advisability of corticosteroid therapy. the predictive value of pulmonary uptake proved to be limited, but there has been renewed interest in 67ga scanning as a diagnostic aid with special attention to characteristic extrapulmonary uptake patterns. review of whole-body 67ga scans in 172 patients with sarcoidosis, 21 with lymphoma, and 51 with other d ... | 2005 | 1952451 |
| the hydrolysis of lucerne cell-wall monosaccharide components by monocultures or pair combinations of defined ruminal bacteria. | the defined ruminal bacterial strains fibrobacter succinogenes s85, ruminococcus flavefaciens fd1, ruminococcus albus 7, butyrivibrio fibrisolvens d1, and bacteroides ruminicola ga33 were grown, in monocultures or as combinations of pair strains, on isolated lucerne cell-walls (cw) as the sole carbohydrate substrate. fibrobacter succinogenes s85 was the dominant strain determining extent of cw hydrolysis in all combinations with s85. the hydrolysis of cellulose, xylan, hemicellulose side-sugars, ... | 1991 | 2030098 |
| introduction of the bacteroides ruminicola xylanase gene into the bacteroides thetaiotaomicron chromosome for production of xylanase activity. | the xylanase gene from the ruminal bacterium bacteroides ruminicola 23 is highly expressed in colonic bacteroides species when carried on plasmid pval-rx. in order to stabilize xylanase expression in the absence of antibiotic selection, the xylanase gene was introduced into the chromosome of bacteroides thetaiotaomicron 5482 by using suicide vector pval-7. xylanase activity in the resulting strain, b. thetaiotaomicron btx, was about 30% of that observed in b. thetaiotaomicron 5482 containing the ... | 1991 | 2036016 |
| plasmid transformation of bacteroides spp. by electroporation. | transformation of bacteroides spp. with a variety of plasmid dnas was accomplished using electroporation. the standard transformation assay system used to deduce the optimal electroporation parameters employed a 50-to 100-fold concentrated cell suspension of mid-logarithmic phase bacteroides fragilis strain 638 and the 5.4-kb clindamycin resistance (ccr) vector, pbi191. a variety of electroporation buffers were used successfully in transformation experiments but of these, 1 mm mgcl2 in 10% glyce ... | 1990 | 2096397 |
| site-specific rate constants for iron removal from diferric transferrin by nitrilotris(methylenephosphonic acid) and pyrophosphate. | the kinetics of iron removal from human serum diferric transferrin by nitrilotris(methylenephosphonic acid) (ntp) and pyrophosphate (ppi) have been studied in 0.1 m, ph 7.4, n-(2-hydroxyethyl)piperazine-n'-2-ethanesulfonate buffer at 25 degrees c using urea/polyacrylamide gel electrophoresis. the four microscopic rate constants required for a complete description of iron removal from the two transferrin metal-binding sites have been measured at 100 mm concentrations of ntp and ppi. there is very ... | 1990 | 2168158 |
| cloning of the yeast sfl2 gene: its disruption results in pleiotropic phenotypes characteristic for tup1 mutants. | we have identified a yeast gene, sfl2 (suppressor gene for flocculation), which complemented a newly isolated sfl2 mutant. this mutation causes asexual cell aggregation. the strain bearing the sfl2 gene disruption exhibited pleiotropic phenotypes characteristic for tup1 mutants. physical mapping and complementation analysis suggested that the cloned sfl2 gene is identical to the tup1 gene. the sfl2 gene encodes a 669-amino acid protein which has domains rich in glutamine, as does the ssn6 protei ... | 1990 | 2197185 |
| [denture stomatitis. its relation to candida albicans]. | one of the most usual complications of the patients that carry a prosthesis is one called stomatitis denture. its etiology is not clear enough. in this work we study ninety people, sixty of them wear prosthesis. we take samples of different places in the mouth to plate and class c. albicans. we look for the relationship of results with the clinical parameters of the patient. | 1990 | 2203365 |
| non-immune hydrops in trisomy-18. diagnosis by vaginosonography and chorionic villus sampling in the first trimester. case report. | 1990 | 2223690 | |
| obligately anaerobic bacterial species isolated from foot-rot lesions in goats. | lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during october 1987 to november 1988. a total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. the anaerobes most frequently isolated belonged to the following genera: bacteroides (80%), peptostreptococcus (63.6%), megasphaera (40%), fusobacterium (29.2%), clostridium (22.5%), propionibacterium (12.5%), eubacterium (11.7%) and leptotrichia (10.8%). ... | 1990 | 2271912 |
| physiology and genetics of xylan degradation by gastrointestinal tract bacteria. | hemicelluloses or xylans are major components (35%) of plant materials. for ruminant animals, about 50% of the dietary xylans are degraded, but only small amounts of xylans are degraded in the lower gut of nonruminant animals and humans. in the rumen, the major xylanolytic species are butyrivibrio fibrisolvens and bacteroides ruminicola. in the human colon, bacteroides ovatus and bacteroides fragilis subspecies "a" are major xylanolytic bacteria. xylans are chemically complex, and their degradat ... | 1990 | 2283426 |
| heterologous expression of the bacteroides ruminicola xylanase gene in bacteroides fragilis and bacteroides uniformis. | a cloned xylanase gene from the ruminal bacterium bacteroides ruminicola 23 was transferred by conjugation into the colonic species bacteroides fragilis and bacteroides uniformis by using the escherichia coli-bacteroides shuttle vector pval-1. the cloned gene was expressed in both species, and xylanase specific activity in crude extracts was found to be at least 1400-fold greater than that found in the b. ruminicola strain. analysis of crude extract proteins from the recombinant b. fragilis by s ... | 1990 | 2323546 |
| effects of the ionophore tetronasin on nitrogen metabolism by ruminal microorganisms in vitro. | the effects of tetronasin on ruminal protein metabolism were investigated in vitro using ruminal fluid from cattle receiving tetronasin in the diet, ovine ruminal fluid from animals not receiving tetronasin and pure cultures of proteolytic ruminal bacteria. ruminal fluid from cattle receiving tetronasin in a predominantly barley diet had lower proteolytic (76% of control, p less than .10) and deaminative (58% of control, p less than .05) activities than controls after 42 d. the effect of deamina ... | 1990 | 2332385 |
| cloning and sequencing of a bacteroides ruminicola b(1)4 endoglucanase gene. | bacteroides ruminicola b(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [cmcase]) that is excreted into the culture supernatant. cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of cmcase (greater than 3 mmol of reducing sugar per mg of protein per min), but its synthesis was repressed by sucrose. b. rumincola did not grow on either ball-milled or acid-swollen cellulose even though the cmcase could hydro ... | 1990 | 2361940 |
| [diagnosis, incidence and clinical significance of premature lh rise/peak in patients of an in vitro fertilization and gamete transfer program]. | in this study we analysed the prevalence and the clinical relevance of premature (related to the day of ovulation induction with hcg) lh rise (lh greater than or equal to 180%, but less than 300% of the mean of all previous values) or peak (lh greater than or equal to 300% of the mean of all previous values): 12% (2/16) of the conception cycles showed a premature lh peak, 44% (7/16) a premature lh rise and 44% (7/17) showed neither a premature lh rise nor a premature lh peak. the pregnancy rate ... | 2007 | 2376305 |
| the survival of subgingival plaque bacteria in an amine fluoride-containing gel. | subgingival plaque samples from 20 patients with chronic inflammatory periodontal disease were exposed to a commercial gel formulation containing 2 amine fluorides. the mic of the gel for these samples ranged from 33 to 260 micrograms/ml with a modal value of 260 micrograms/ml. in each sample, the most resistant organisms (i.e., those organisms surviving at one doubling dilution below the mic) were identified. 33 such organisms were isolated, of which 22 (67%) were strict anaerobes, and 25 (75%) ... | 1990 | 2387913 |
| amylolytic activity of selected species of ruminal bacteria. | a variety of species of ruminal bacteria were screened for the ability to grow in starch-containing medium and produce amylase. of those tested, the highest levels of amylase were produced by streptococcus bovis jb1 and ruminobacter amylophilus h18. other strains that grew well on starch and produced amylase included butyrivibrio fibrisolvens a38 and 49 and bacteroides ruminicola 23 and b14. varying the carbohydrate source provided for growth resulted in changes in the growth rate and level of a ... | 1988 | 2454075 |
| [anaerobic bacteria in intracranial suppuration]. | in four cases of intracranial suppurative disease, studied in the laboratory of anaerobic bacteria of institute pasteur of algiers, three cases are caused by anaerobic bacteria and one remain sterile. two of them are empyema with unknown origin and the others are brain abscess with bilateral chronic ear infection to one. in the three cases, gram negative anaerobic bacilli were grown in culture: bacteroides asaccharolyticus, bacteroides vulgatus and bacteroides ruminicola brevis. in two cases, th ... | 1989 | 2489404 |
| [effect of systemic oral administration of tetracycline on experimental gingivitis in golden hamsters]. | local and systemic administrations of tetracycline have been used in human periodontal treatment for conditions including juvenile periodontitis and rapidly progressive periodontitis, although microbiological effects of the treatment have not been clear. the effect of systemic oral administration of tetracycline on subgingival bacteria in experimental periodontal disease in hamsters as an animal model has not yet been reported. the aim of this study was to investigate changes in subgingival bact ... | 1989 | 2489558 |
| morphological response of mycoplasma to fleroxacin. | quinolones exhibit partially reduced activity in vitro against genital mycoplasma. therefore, the morphological response to fleroxacin, a difluorinated quinolone, by mycoplasma hominis was studied. even at subinhibitory concentrations the ultrastructural investigations revealed distinct alterations such as cytolysis, vacuole and cell "ghost" formation. the typical dumbbell-shaped cells observed with monofluorinated compounds were not found, which may indicate a qualitatively different mode of ac ... | 1989 | 2515157 |
| specificity spillover at the hormone receptor--exploring its role in human disease. | 1989 | 2537464 | |
| electroporation induced transformation of bacteroides ruminicola and bacteroides uniformis by plasmid dna. | frequencies of greater than 10(5) transformants per microgram dna were achieved in bacteroides ruminicola f101 by electroporation of cells under anaerobic conditions, using the 19.5 kbp tetracycline resistance plasmid prri4. similar procedures gave frequences of 10(6) erythromycin resistant transformants per microgram dna with the shuttle plasmid pdp1 (19 kbp) in bacteroides uniformis. transformation of b. uniformis occurred at a far lower frequency (10(3) micrograms) when pdp1 dna was derived f ... | 1989 | 2599352 |
| isolation of an endoglucanase gene from bacteroides ruminicola subsp. brevis. | a gene coding for endo-1, 4-beta-glucanase activity has been isolated from bacteroides ruminicola subsp. brevis by cloning in escherichia coli. after restriction mapping of a 6.4 kb insert, a 2.2 kb dna fragment was sub-cloned in puc19 to produce the enzymically active clone pjw3. recloning of the gene fragment in the reverse orientation in puc18 (clone pjw4) indicated that a gene promoter was present in the cloned fragment and was able to function in e. coli. the clone pjw4 displayed increased ... | 1989 | 2628545 |
| health impact of renal disease due to nephrotoxicity. | both acute and chronic renal failure are reported in the context of nephrotoxicity. this overview of some major nephrotoxins reflects both the magnitude and the cost of this preventable health problem. aminoglycosides and other antibiotics, contrast media and nonsteroidal anti-inflammatory drugs are the best documented nephrotoxins related to acute renal failure. analgesic nephropathy is the best known drug-induced chronic renal disease. the cost of renal failure due to nephrotoxicity is not eas ... | 2009 | 2650034 |
| cloning and expression in escherichia coli of a xylanase gene from bacteroides ruminicola 23. | a gene coding for xylanase activity in the ruminal bacterial strain 23, the type strain of bacteroides ruminicola, was cloned into escherichia coli jm83 by using plasmid puc18. ab. ruminicola 23 genomic library was prepared in e. coli by using bamhi-digested dna, and transformants were screened for xylanase activity on the basis of clearing areas around colonies grown on remazol brilliant blue r-xylan plates. six clones were identified as being xylanase positive, and all six contained the same 5 ... | 1989 | 2658806 |
| urapidil-induced hemodynamic changes in humans. | in hypertensive patients as well as in normal subjects urapidil has a hypotensive action. this is mainly mediated by a peripheral alpha 1-adrenoceptor blockade with a decrease in systemic vascular resistance; in addition, during acute animal experiments a centrally mediated hypotensive action was demonstrated, possibly by 5-hydroxytryptamine1a (5-ht1a)-receptor stimulation. studies in humans showed an increase in cardiac output, which was not always significant; it did result either from an incr ... | 2008 | 2667309 |
| immunoprecipitation of cell lysates with rap-5 does not specifically detect ras oncogene product p21. | in the process of developing accurate quantitation of the ras protein (p21), we have screened available anti-ras antibodies for their utility in immunoprecipitation. immunoprecipitation with the anti-ras antibody rap-5 consistently failed to precipitate p21 present in two different cell lines (hsic-5 and mcf-7), but did precipitate numerous other proteins present in these cell lines. specificity in immunoprecipitation could not be achieved by varying the concentration of rap-5. in addition, immu ... | 2009 | 2687155 |
| [sweet's syndrome]. | the authors describe a clinical case of sweet's syndrome in a 41-year-old man. from the clinical-instrumental tests carried out a paraneoplastic syndrome is excluded. | 1989 | 2698381 |
| more monensin-sensitive, ammonia-producing bacteria from the rumen. | two monensin-sensitive bacteria which utilized carbohydrates poorly and grew rapidly on amino acids were isolated from the bovine rumen. the short rods (strain sr) fermented arginine, serine, lysine, glutamine, and threonine rapidly (greater than 158 nmol/mg of protein per h) and grew faster on casein digest containing short peptides than on free amino acids ().34 versus 0.29 h(-1)). gelatin hydrolysate, an amino acid source containing an abundance of long peptides, was unable to support growth ... | 1989 | 2757371 |
| direct measurement of basolateral membrane potentials from cells of the macula densa. | at the present time, little is known concerning the electrophysiology of the cells of the macula densa and whether or not these cells are electrically responsive to alterations in luminal fluid composition. to investigate this issue, cortical thick ascending limbs (ctal) containing macula densa and attached glomeruli were dissected from rabbit kidney and the ctal perfused in vitro. basolateral membrane potential (vbl) was measured with microelectrodes in macula densa cells and, for comparison, i ... | 1989 | 2782426 |
| cocaine use by senior medical students. | the authors studied 589 senior medical students' attitudes about and use of cocaine. reported use was 36% in the students' lifetimes, 17% in the past year, and 6% in the past month. overall, these rates were lower than those of an age-matched cohort. | 2009 | 2784037 |
| the dilation of arterioles in frog submaxillaris muscle caused by visible light. | transillumination of submaxillaris muscle with incandenscent lamp led to arteriolar dilation if the illuminance exceeded 1-2 x 10(3) lux or reached the time-mean value of 3 x 10(4) lux when a flash lamp was used (pulse duration 10 microseconds, repetition rate 50 s-1). the temperature increase within a light spot on the muscle did not exceed 0.1 degrees c, visible light being the only reason of the dilation of arterioles. at 6.3 x 10(4) lux (incandescent lamp, green filter) the latency of dilati ... | 1989 | 2786511 |
| spontaneous tumors in f-344/jcl rats. | the age-related incidence of spontaneously occurring neoplastic and non-neoplastic lesions in untreated f-344/jcl rats, used as controls in carcinogenicity testing, were studied from the histological examination of tissues from 469 males and 354 females. the incidence of spontaneous tumors was 83.2% in the males and 71.2% in the females. the most common neoplasms were leukemia (males: 24.3%, females: 24.0%), pituitary adenoma (males: 16.0%, females: 45.2%), pheochromocytoma (males: 14.7%, female ... | 1989 | 2810400 |
| the versatility of hydroxyapatite blocks in maxillofacial surgery. | there is considerable interest at present in the potential role of calcium hydroxyapatite as a biomaterial for bone augmentation in oral and maxillofacial surgery. most of the published work has been on the particulate form, mainly in alveolar ridge procedures. however, hydroxyapatite is also available as solid or porous blocks. the blocks are more predictable in their use than are the particles, and are adaptable and versatile. this paper reviews the biological behaviour of the material and dis ... | 2010 | 2825758 |
| plasmid-associated transfer of tetracycline resistance in bacteroides ruminicola. | tetracycline resistance was transferred at frequencies between 10(-7) and 10(-6) per recipient cell in anaerobic matings between two strains of the strictly anaerobic rumen bacterium bacteroides ruminicola. the donor strain, 223/m2/7, was a multiple-plasmid-bearing tetracycline-resistant strain from the ovine rumen, and the recipient, f101, was a rifampin-resistant mutant of b14, a bovine strain belonging to b. ruminicola subsp. brevis. resistance transfer could occur in the presence of dnase, b ... | 1988 | 2837147 |
| monoclonal-antibody-mediated enzyme-linked immunosorbent assay for detection of reticuloendotheliosis viruses. | an enzyme-linked immunosorbent assay (elisa) is described for the detection of reticuloendotheliosis viruses (revs). the assay uses a mixture of monoclonal antibodies (mcas) prepared against a 62-kilodalton rev envelope glycoprotein (gp62) to capture antigen, rabbit anti-rev serum as detection antibody, and peroxidase-conjugated anti-rabbit igg as indicator antibody. the mcas were reactive with rev strain t, chick syncytial virus, and duck infectious anemia virus but unreactive against marek's d ... | 1988 | 2838016 |
| [therapy of aids]. | therapeutic strategies in the management of aids should aim at an inhibition of the replication and spread of hiv; the reconstitution of immune functions; an effective chemotherapy of opportunistic infections and tumors; and the prevention of new opportunistic infections. at present, these goals can be only partially achieved and where beneficial therapeutic results are obtained they are only transient in nature. antiretroviral therapy and chemotherapy of infections have, however, resulted in so ... | 1988 | 2849247 |
| human pharmacokinetic and pharmacodynamic studies on ro31-1118, a new beta-adrenoceptor antagonist. | the pharmacokinetic and pharmacodynamic effects of ro31-1118 were examined in groups of healthy volunteers. in three subjects given 10 mg of [14c]-ro31-1118 orally, peak levels of radioactivity (84 +/- 5 ng/ml) were 16 times those of the parent drug (approximately 5 ng/ml). very little parent drug was recovered in the urine, although recovery of total radioactivity was nearly 80% in the urine by day 5. in five subjects studied after both oral and intravenous administration of 20 mg ro31-1118 the ... | 1985 | 2859047 |
| problem-oriented article summary. | 2010 | 2863481 | |
| interleukin-2 receptor-inducing factor(s) in adult t cell leukemia. | the expression of the interleukin-2 receptor (il-2-r) is regulated by transcriptional and post-transcriptional mechanisms. il-2-r gene expression is induced by pharmacological agents including calcium ions, phorbol esters such as phorbol myristate acetate (pma) and forskolin (fk), a direct activator of adenylate cyclase. htlv-i(+) leukemic t cells and t cell lines from patients with adult t cell leukemia (atl) continuously expressed il-2-r without production of il-2. however, there was no abnorm ... | 1987 | 2893506 |
| use of reca protein to enrich for homologous genes in a genomic library. | reca protein-coated probe has been utilized to enrich genomic digests for desired genes in order to facilitate cloning from genomic libraries. using a previously cloned hla-b27 gene as the reca-coated enrichment probe, we obtained a mean 108x increase in the ratio of specific to nonspecific plaques in lambda libraries screened for b27 variant alleles of estimated 99% homology to the probe. class i genes of lesser homology were less enriched: 6.7x for non-b27 genes of estimated greater than 95% h ... | 2010 | 2901713 |
| a new rflp at the human vitamin-d binding protein (hdbp) locus. | 1988 | 2901718 | |
| oophoropexy and the management of hodgkin's disease. a reevaluation of the risks and benefits. | female patients with hodgkin's disease who undergo staging laparotomy frequently have oophoropexy performed to preserve both fertility and hormone production. because of recent changes in therapy favoring systemic chemotherapy rather than total nodal irradiation for patients with stage iii hodgkin's disease, the need for oophoropexy may be less than previously described. thirty-nine women of childbearing age underwent laparotomy at the university of north carolina, chapel hill, from 1970 to 1984 ... | 2010 | 2943253 |
| [2 cases of pneumoblastoma with pulmonary metastases responsive to chemotherapy]. | 2010 | 2950479 | |
| clinical and metabolic study of a new pill containing 20 mcg ethinylestradiol plus 0.150 mg desogestrel. | the clinical and metabolic effects of a short-term treatment with a combination contraceptive pill containing 0.150 mg desogestrel and 20 mcg ethinylestradiol were evaluated in a group of 17 healthy women. in spite of the low estrogen content, the pill exerted a good cycle control and the incidence of irregular bleedings was low. the minor side effects commonly associated with oral contraceptive (oc) use rarely occurred, and an improvement of premenstrual symptoms was reported during pill intake ... | 2010 | 2956055 |
| [clinical use of pantethine by parenteral route in the treatment of hyperlipidemia]. | recent investigations have confirmed the effectiveness and the excellent tolerability of pantethine, a derivative of pantetheine, an essential part of the acetylation coenzyme coa, administered p.o., in normalizing the blood lipid concentrations of patients with hyperlipidemias. a group of 18 patients with hyperlipidemias (9 m, 9 f), with an average age of 52.6 years, was submitted to pantethine parenteral treatment. after a 20 days wash-out, pantethine (400 mg/day; bid) was administered intramu ... | 2010 | 2970754 |
| fermentation of peptides and amino acids by a monensin-sensitive ruminal peptostreptococcus. | a monensin-sensitive ruminal peptostreptococcus was able to grow rapidly (growth rate of 0.5/h) on an enzymatic hydrolysate of casein, but less than 23% of the amino acid nitrogen was ever utilized. when an acid hydrolysate was substituted for the enzymatic digest, more than 31% of the nitrogen was converted to ammonia and cell protein. coculture experiments and synergisms with peptide-degrading strains of bacteroides ruminicola and streptococcus bovis indicated that the peptostreptococcus was u ... | 1988 | 2975156 |
| a gene expressed in undifferentiated vegetative dictyostelium is repressed by developmental pulses of camp and reinduced during dedifferentiation. | we describe the gene m4-1, whose unique pattern of developmental expression will allow us to study the molecular mechanisms controlling expression in undifferentiated cells in addition to repression in response to camp during development and reinduction during dedifferentiation. m4-1 is a dictyostelium gene expressed in the undifferentiated cell. we have shown that m4-1 continues to be expressed very early during the developmental cycle but is repressed at a later stage of development, at a time ... | 2010 | 3010312 |
| [analysis of the spectrum of acth-immunoreactive peptides in the brain of inbred mice]. | the acth-immunoreactive peptides (ip) distribution in the acid extract of brain and plasma fractions of c57bl/6, balb/c and f1 (c57bl/6 x balb/c) mice was investigated. ip were isolated by high-performance liquid chromatography. interstrain differences in the ip spectrum were found in intact mice and mice after exposure to stress in the "open-field" test. | 2010 | 3017474 |
| increased gastric cholinergic activity evoked by 5-hydroxy-l-tryptophan in the rat. | in gastrointestinal tissues such as rat stomach, exogenous 5-hydroxytryptamine (5ht) has little or no ability to affect nerve activity. however, endogenous 5ht might act differently, and this was investigated by stimulating 5ht synthesis using 5-hydroxy-l-tryptophan (5htp). in longitudinal strips of rat forestomach, 5htp (50 and 500 microm) increased cholinergically mediated contractions evoked by electrical field stimulation, probably by facilitating acetylcholine release; contractions evoked b ... | 1986 | 3019729 |
| increased gastric cholinergic activity evoked by 5-hydroxy-l-tryptophan in the rat. | in gastrointestinal tissues such as rat stomach, exogenous 5-hydroxytryptamine (5ht) has little or no ability to affect nerve activity. however, endogenous 5ht might act differently, and this was investigated by stimulating 5ht synthesis using 5-hydroxy-l-tryptophan (5htp). in longitudinal strips of rat forestomach, 5htp (50 and 500 microm) increased cholinergically mediated contractions evoked by electrical field stimulation, probably by facilitating acetylcholine release; contractions evoked b ... | 1986 | 3019729 |
| seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus). | the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ... | 1987 | 3030193 |
| relationship of histologic grade of hepatocellular carcinoma (hcc) to tumor size, and demonstration of tumor cells of multiple different grades in single small hcc. | the histologic features of 65 surgically resected cases of hepatocellular carcinoma (hcc) were studied, and the cellular differentiation was graded from i to iv according to edmondson-steiner's classification. hcc cells of 2 or more grades were seen in 31 (47.7%) of the 65 cases. there was no significant difference in the proportion of hcc composed of tumor cells of more than one histologic grade between hccs with association with liver cirrhosis and without cirrhosis. among these 31 cases, extr ... | 2011 | 3033422 |
| estradiol-binding proteins from mycelial and yeast-form cultures of paracoccidioides brasiliensis. | paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis, causes disease much more frequently in men than it does in women, suggesting that the hormonal milieu of the host might influence p. brasiliensis pathogenicity. we recently demonstrated that cytosol from yeast cultures of p. brasiliensis contains a high-affinity, low-capacity, tritiated 17 beta-estradiol [( 3h]estradiol)-binding protein. estradiol and, to a lesser degree, diethylstilbestrol (des), inhibited the transfo ... | 1986 | 3079729 |
| [occupational health program for the workers of health centers]. | 2011 | 3105075 | |
| oesophageal pain exacerbated by propranolol. | 2011 | 3113570 | |
| susceptibility and resistance of ruminal bacteria to antimicrobial feed additives. | susceptibility and resistance of ruminal bacterial species to avoparcin, narasin, salinomycin, thiopeptin, tylosin, virginiamycin, and two new ionophore antibiotics, ro22-6924/004 and ro21-6447/009, were determined. generally, antimicrobial compounds were inhibitory to gram-positive bacteria and those bacteria that have gram-positive-like cell wall structure. mics ranged from 0.09 to 24.0 micrograms/ml. gram-negative bacteria were resistant at the highest concentration tested (48.0 micrograms/ml ... | 1987 | 3116929 |
| regulation of beta-glucosidase in bacteroides ruminicola by a different mechanism: growth rate-dependent derepression. | bacteroides ruminicola b(1)4, a predominant ruminal and cecal bacterium, was grown in batch and continuous cultures, and beta-glucosidase activity was measured by following the hydrolysis of p-nitrophenyl-beta-glucopyranoside. specific activity was high when the bacterium was grown in batch cultures containing cellobiose, mannose, or lactose (greater than 286 u/g of protein). activity was reduced approximately 90% when the organism was grown on glucose, sucrose, fructose, maltose, or arabinose. ... | 1987 | 3122655 |