Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| bacterial phylogenetic clusters revealed by genome structure. | current bacterial taxonomy is mostly based on phenotypic criteria, which may yield misleading interpretations in classification and identification. as a result, bacteria not closely related may be grouped together as a genus or species. for pathogenic bacteria, incorrect classification or misidentification could be disastrous. there is therefore an urgent need for appropriate methodologies to classify bacteria according to phylogeny and corresponding new approaches that permit their rapid and ac ... | 1999 | 10542177 |
| serological relationship of some v-factor dependent pasteurellaceae (haemophilus sp.) from guineapigs and rabbits. | culture of guinea pig and rabbit respiratory tracts for bacteria using x- (haemin) and v- (nad) factor in agar media detected infection by v-factor dependent pasteurellaceae (haemophilus sp.) in three colonies of guinea pigs and a group of rabbits. the 12 haemophilus strains comprised three api nh codes classed as haemophilus parainfluenzae and two codes classed as haemophilus aphrophilus/paraphrophilus. six cell wall lipid profiles were detected, but these were not related to api nh codes. both ... | 1999 | 10759399 |
| dual infection with pneumocystis carinii and pasteurella pneumotropica in b cell-deficient mice: diagnosis and therapy. | the clinical presentation, diagnosis, histopathologic findings, and elimination of dual respiratory tract infection with pasteurella pneumotropica and pneumocystis carinii were studied in 100 adult barrier-reared c.b17 and mrl- lpr mice homozygous for a targeted mutation of the jh region of the immunoglobulin heavy chain. | 2000 | 10987669 |
| antibiotic resistance in bacterial isolates from laboratory animal colonies naive to antibiotic treatment. | antiobiogrammes were made of a number of isolates of staphylococcus aureus, escherichia coli and pasteurella pneumotropica derived from rodent, rabbit or minipig colonies never treated with antibiotics. for s. aureus no differences between rats and mice were found in the percentage of resistant isolates. gentamicin and erythromycin were found to be the most efficient, while the highest percentages of resistance were found to be against penicillins and sulphonamides. in general, the results from ... | 2000 | 11072863 |
| hamster bite peritonitis: pasteurella pneumotropica peritonitis in a dialysis patient. | we report the first case of pasteurella pneumotropica peritonitis in a peritoneal dialysis patient. this rare infection was the result of contamination of the dialysis tubing by a pet hamster. we stress the importance of household pets as a source of infection in the peritoneal dialysis population. | 2000 | 11095007 |
| detection and elimination of contaminating microorganisms in transplantable tumors and cell lines. | as a quarantine of biological materials, we tested 96 transplantable tumors and cell lines for contamination with microorganisms in a mouse antibody production (map) test, enzymatic assay and microbiological culture. contamination with lactic dehydrogenase elevating virus (ldv), mycoplasmas and pasteurella pneumotropica was detected. a considerable difference in the contamination rate was observed between in vivo- and in vitro- propagated tumors. ldv in the tumors could be eliminated by both in ... | 2000 | 11109559 |
| outbreak of pasteurella pneumotropica in a closed colony of stock-cd28(tm1mak) mice. | fifteen mice with pasteurella pneumotropica orbital abscesses were noted in mice that were homozygous for a targeted cd28 gene mutation. only one mouse heterozygous for the cd28 mutation was affected. according to phenotypic reactions and 16s rdna sequencing, the isolates were most similar to biotype heyl. this article provides evidence for an immunologic basis of susceptibility to p. pneumotropica infection. fifteen mice with pasteurella pneumotropica orbital abscesses were noted in mice that w ... | 2000 | 11178314 |
| mhcii, tlr4 and nramp1 genes control host pulmonary resistance against the opportunistic bacterium pasteurella pneumotropica. | mhcii, tlr4, and nramp1 genes are each independently important in pulmonary immunity. to determine the effect of these genes on host resistance, mice carrying various combinations of functional alleles for these three genes were experimentally challenged with the opportunistic bacterium, pasteurella pneumotropica. mhcii-/-, tlr4d/d, and nramp1s/s mice were significantly more susceptible to experimental infections by p. pneumotropica after intranasal challenge compared to mice carrying functional ... | 2001 | 11261784 |
| survival and transmissibility of pasteurella pneumotropica. | survival has been determined for pasteurella pneumotropica on various surfaces found in an animal room at 23+/-1 degrees c and 50+/-10% relative humidity. longest survival (120 min) was found on mouse hair, shortest (< 30 min) on laboratory coat fabric. transmission experiments were performed using sentinel animals in order to evaluate the efficiency of their use for the detection of p. pneumotropica in quarantined mice. in sentinels exposed to infected mice by close contact, p. pneumotropica wa ... | 2001 | 11315166 |
| septicemia due to pasteurella pneumotropica: 16s rrna sequencing for diagnosis confirmation. | bacteremia due to pasteurella pneumotropica occurs infrequently. we report a case of septicemia in a 72-year-old woman who had no underlying illness. the microorganism was isolated from 10 blood cultures and identified by conventional and molecular methods. this is the first reported case of p. pneumotropica septicemia in an immunocompetent patient. the history of p. pneumotropica diseases in animals and humans and their varied clinical features are reviewed. | 2002 | 11825998 |
| bacteremia in children: etiologic agents, focal sites, and risk factors. | a prospective study was carried out on 210 cases of children under 10 years of age with fever. cases of gastroenteritis, respiratory tract infections, and suspected sepsis in children seen or admitted to the pediatric hospital were studied. clinical and microbiological data were recorded in a questionnaire or obtained from patient medical records. most of the children with septicemia (71.3 per cent) were less than 1 year old. focal source of bacteremia was gastroenteritis (40.4 per cent), pneumo ... | 2001 | 11827304 |
| prevalence of a novel capsule-associated lipoprotein among pasteurellaceae pathogenic in animals. | capsular serotype a strains of pasteurella multocida of avian origin express a 40-kda lipoprotein (plp-40) thought to attach the extracellular polysaccharide to the cell surface. the objective of the present study was to assess the prevalence of plp-40 in p. multocida strains of disparate serotypes and host origins, as well as other pathogenic members of the family pasteurellaceae. exponential-phase reference and clinical isolates were radiolabeled with [3h]-palmitate, lysed to obtain whole-cell ... | 2002 | 11910502 |
| elimination of pasteurella pneumotropica from a contaminated mouse colony by oral administration of enrofloxacin. | enrofloxacin, a fluoroquinolone bactericidal antibiotic, was administered in an attempt to eradicate pasteurella pneumotropica (p. pneumotropica) from a contaminated mouse colony. contaminated mice, maintained within 4 animal rooms, were administered enrofloxacin in drinking water at a daily dosage of 25.5 mg/kg for 2 weeks. following one week of enrofloxacin treatment, mice were selected randomly from each room and examined for p. pneumotropica. this procedure was repeated two or three times un ... | 2002 | 12221935 |
| toll-like receptor 4-positive macrophages protect mice from pasteurella pneumotropica-induced pneumonia. | this study investigates toll-like receptor 4 (tlr4)-positive macrophages in early recognition and clearance of pulmonary bacteria. tlr4 is a trans-membrane receptor that is the primary recognition molecule for lipopolysaccharide of gram-negative bacteria. the tlr4(lps-del) mouse strains c57bl10/scn (b10) and stock abb(tm1) tlr4(lps-del) slc11a1(s)(b10 x c2d) are susceptible to pulmonary infections and develop pneumonia when naturally or experimentally infected by the opportunistic bacterium past ... | 2003 | 12540543 |
| experimental evaluation of cross-contamination between cryotubes containing mouse 2-cell embryos and murine pathogens in liquid nitrogen tanks. | it has been suspected that embryos stored in liquid nitrogen tanks may become contaminated with murine pathogens, if some pathogens had been introduced to the tanks accidentally. to examine this, we stored tubes containing embryos with tubes containing mouse hepatitis virus (mhv) or pasteurella pneumotropica in liquid nitrogen tanks and examined whether progeny mice derived from the embryos were contaminated with the pathogens or not. after storing for 6 months or 1 year the frozen embryos were ... | 2003 | 12638239 |
| evaluation of a forced-air-ventilated micro-isolation system for protection of mice against pasteurella pneumotropica. | studies to date have established that the physical environment inside cages can be controlled adequately by setting the intra-cage ventilation at 60 air changes per hour in a forced-air-ventilated micro-isolation system (fvmis). in this study, the capability of fvmis to prevent inter-cage transmission of microorganisms was evaluated using pasteurella pneumotropica as a reference microorganism. one fvmis rack and a conventional rack were used, and cages with mice positive for p. pneumotropica and ... | 2003 | 12806889 |
| assessing the risk of transmission of three infectious agents among mice housed in a negatively pressurized caging system. | previous studies from our institution have shown that ventilated caging run at negative pressure to a mouse room dramatically reduced exposure of personnel to the major mouse allergen, mus m 1. the current study was designed to determine whether negative cage ventilation posed an inordinate risk for spread of infectious agents between cages and/or racks. b6;129s-tnfsf5(tm1imx)/j (tnf) mice, which were naturally and persistently infected with pneumocystis carinii, helicobacter bilis, and pasteure ... | 2003 | 14615955 |
| studies on the occurrence of gram-negative bacteria in ticks: ixodes ricinus as a potential vector of pasteurella. | a total of 372 ixodes ricinus ticks (101 females, 122 males, and 149 nymphs) collected by flagging in 6 mixed woodlands of eastern poland were examined by culture for the presence of internal gram-negative bacteria other than borrelia burgdorferi. adult ticks were examined in pools of 2 specimens each and nymphs were examined in pools of 3-5 specimens each. ticks were disinfected in 70 % ethanol and homogenized in 0.85% nacl. the diluted homogenate was inoculated onto 3 kinds of agar media: buff ... | 2004 | 15627343 |
| rapid and accurate identification of human isolates of pasteurella and related species by sequencing the soda gene. | the identification of pasteurella and related bacteria remains a challenge. here, a 449- to 473-bp fragment (soda(int)) internal to the soda gene, encoding the manganese-dependent superoxide dismutase, was amplified and sequenced with a single pair of degenerate primers from the type strains of pasteurella (18 strains), gallibacterium (1 strain), and mannheimia (5 strains) species. the soda(int)-based phylogenetic tree was in general agreement with that inferred from the analysis of the correspo ... | 2005 | 15872260 |
| identification procedure for pasteurella pneumotropica in microbiologic monitoring of laboratory animals. | discrepancies have been recognized in the identification of pasteurella pneumotropica between testing laboratories. to determine the causes of the differences and to propose a reliable identification procedure for p. pneumotropica, a working group was organized and 69 isolates identified or suspected as p. pneumotropica were collected from 8 laboratories in japan. these isolates were examined by colony morphology, gram-staining, the slide agglutination test using two antisera (atcc35149 and mar) ... | 2005 | 15897620 |
| correlation of results of pulmonary computed tomography and pathologic findings in mice with pasteurella-induced pneumonia. | to determine correlation between results of computed tomography (ct) versus pathologic examination for determining the volume percentage of affected lung in mice experimentally infected with pasteurella pneumotropica. | 2005 | 15938071 |
| mouse infection models for space flight immunology. | several immunological processes can be affected by space flight. however, there is little evidence to suggest that flight-induced immunological deficits lead to illness. therefore, one of our goals has been to define models to examine host resistance during space flight. our working hypothesis is that space flight crews will come from a heterogeneous population; the immune response gene make-up will be quite varied. it is unknown how much the immune response gene variation contributes to the pot ... | 2005 | 16101105 |
| genetic diversity on 16s rdna sequence and phylogenic tree analysis in pasteurella pneumotropica strains isolated from laboratory animals. | to reveal the genetic diversity of pasteurella pneumotropica, the 16s rdna sequence and multiple alignments were performed for 35 strains (from 17 mice, 13 rats, 3 hamsters, 1 rabbit, and 1 guinea pig) identified as p. pneumotropica using a commercial biochemical test kit or pcr test and two reference strains (atcc 35149 and cnp160). each strain showed a close similarity with one of the following organisms: p. pneumotropica (m75083), bisgaard taxon22 (ay172726), pasteurella sp. mccm00235 (af2243 ... | 2005 | 16187154 |
| rat strains differ in antibody response to natural haemophilus species infection. | antibody response to haemophilus species in rat strains was monitored by enzyme-linked immunosorbent assay (elisa) using antigens of two haemophilus strains and a pasteurella pneumotropica strain. five rat strains from a breeding colony naturally infected by haemophilus were significantly different in elisa antibody activity and in the number of seropositive animals. bn and rp rats were (relatively) high and low responders, respectively and buf, lew and wag rats were intermediate. in a second st ... | 2005 | 16197708 |
| evaluation of antigen panels for elisa monitoring of mouse colonies for antibodies to pasteurellaceae. | pasteurellaceae infection in mice may be monitored by the detection of serum antibody using enzyme-linked immunosorbent assay (elisa). we re-evaluated our standard antigen panel comprising pasteurella pneumotropica and a v-factor requiring haemophilus species (strain h21) by studying their serological relationship with actinobacillus muris and 'haemophilus influenzae-murium'. serologically, a. muris and 'h. influenzae-murium' were found to be unrelated and to differ from p. pneumotropica and hae ... | 2006 | 16600079 |
| [subacute bacterial endocarditis due to pasteurella pneumotropica. case report]. | endocarditis due to pasteurella pneumotropica are very rarely described. we report a new case of bacterial endocarditis in a 43 years-old patient with mitral stenosis. the patient was admitted to the hospital for lethargy, malaise and hemiparesis. on physical examination, a new systolic murmur was found. transthoracic echocardiography revealed a vegetation on the mitral valve. three blood culture sets were drawn and after 24 hours of incubation, the last two sets yielded pasteurella pneumotropic ... | 2005 | 16610170 |
| molecular typing of pasteurella pneumotropica isolated from rodents by amplified 16s ribosomal dna restriction analysis and pulsed-field gel electrophoresis. | a total of 52 isolates of pasteurella pneumotropica obtained from rodents were examined for their genetic heterogeneity. on the basis of dna restriction analysis, including amplified 16s ribosomal dna restriction analysis (ardra) and pulsed-field gel electrophoresis (pfge), differences were identified among the isolates. ardra typing with hae iii revealed 4 different banding patterns of the p. pneumotropica isolates. eighty-two percent of the 23 isolates identified as a-1 were derived from mice, ... | 2006 | 16625048 |
| phylogenetic relationship of pasteurella pneumotropica isolates from laboratory rodents based on 16s rdna sequence. | a 1344 bp fragment of the 16s ribosomal dna (rdna) sequence was used to determine the genetic relationship of pasteurella pneumotropica isolates from laboratory rodents. a total of 30 nucleotide sequences of p. pneumotropica, including 24 wild strains, 3 reference strains, and 3 nucleotide sequences deposited in genbank, were examined for heterogeneity of their 16s rdna sequences. phylogenetic analysis based on 16s rdna sequence discriminated 5 types of branching lineages. of these 5 types, 3 ty ... | 2006 | 16820726 |
| [pasteurella pneumotropica produces regression of human tumors transplanted in immunodeficiency mice]. | the technique of human tumor cell line transplantation in immunodeficient mice is used worldwide as a model for cancer research. in accordance with international recommendations, animals used in biomedical research should be free of microorganisms which can interfere in experimental results; including pasteurella pneumotropica. the object of this study was to evaluate the interference produced by p. pneumotropica in the human adenocarcinoma cell line a549 transplanted in n:nih(s)-nu mice. a tota ... | 2006 | 16871912 |
| phylogenetic analysis of isolates of pasteurella pneumotropica from laboratory animals based on the gyrb gene sequence. | phylogenetic analysis using the gyrb sequence was performed to investigate the genetic relevance among 49 isolates of p. pneumotropica. in the phylogeny, the isolates were clearly classified into three groups as follows: group a for the isolates of biotype jawetz derived from mice, group b for the isolates of biotype jawetz derived from rats, and group c for the isolates of biotype heyl. these results suggest that the gyrb sequence of p. pneumotropica differs between the isolates of two biotypes ... | 2006 | 17090967 |
| a specific polymerase chain reaction based on the gyrb gene sequence and subsequent restriction fragment length polymorphism analysis of pasteurella pneumotropica isolates from laboratory mice. | for a molecular identification and typing tool, we developed a specific polymerase chain reaction (pcr) based on the gyrb gene sequence and a subsequent restriction fragment length polymorphism (rflp) analysis using the products amplified from the specific pcr to facilitate discrimination of biotypes of pasteurella pneumotropica from laboratory mice. appropriate pcr products, a 1039-basepair fragment for biotype jawetz and a 1033-basepair fragment for biotype heyl, were amplified by use of the p ... | 2007 | 17343354 |
| ultrastructural characteristics of the external surfaces of pasteurella pneumotropica from mice and pasteurella multocida from rabbits. | the surface structures of the cells of pasteurella pneumotropica from mice and pasteurella multocida from rabbits were examined by transmission electron microscopy after ruthenium red staining and polycationic ferritin labelling. p. pneumotropica strains atcc 35149 and k 79114 had slight extracellular fibrous materials associated with cell walls with ruthenium red staining. ferritin labelling method revealed thick strands or sparsely ferritin-labelled materials on the cell surface of the strains ... | 2007 | 17430628 |
| cross-reactive adaptive immune response to oral commensal bacteria results in an induction of receptor activator of nuclear factor-kappab ligand (rankl)-dependent periodontal bone resorption in a mouse model. | the present study examined whether induction of an adaptive immune response to orally colonizing non-pathogenic pasteurella pneumotropica by immunization with the phylogenetically closely related bacterium, actinobacillus actinomycetemcomitans, can result in periodontal bone loss in mice. | 2007 | 17488448 |
| comparison of the in vitro susceptibility of rodent isolates of pseudomonas aeruginosa and pasteurella pneumotropica to enrofloxacin. | the objectives of this study were to determine and compare the in vitro enrofloxacin susceptibility of 94 pseudomonas aeruginosa isolates obtained from enrofloxacin-treated and untreated mice and that of 40 pasteurella pneumotropica strains and also to assess the efficacy and effects of enrofloxacin treatment of laboratory mice. the minimum inhibitory concentrations (mics) of enrofloxacin against all the ps. aeruginosa isolates were in the range of 1 to 4 microg/ml, whereas those against all the ... | 2007 | 17823403 |
| experimental infection studies of pasteurella pneumotropica and v-factor dependent pasteurellaceae for f344-rnu rats. | to investigate the pathogenicities of p. pneumotropica (pp) and v-factor dependent pasteurellaceae (vfdp) in immunodeficient rats, experimental infections of f344-rnu rats were performed using 3 strains (atcc 35149, cnp 160 and rpz) of pp and 4 strains (v6, v7, v8 and v9) of vfdp. four animals per experimental group were inoculated twice on day 0 and post-inoculation day (pid) 14 with bacterial suspension intranasally. two animals from each group were sacrificed on pid 60 and 120, and examined. ... | 2008 | 18256519 |
| co-infection of respiratory bacterium with severe acute respiratory syndrome coronavirus induces an exacerbated pneumonia in mice. | sars-cov grows in a variety of tissues that express its receptor, although the mechanism for high replication in the lungs and severe respiratory illness is not well understood. we recently showed that elastase enhances sars-cov infection in cultured cells, which suggests that sars development may be due to elastase-mediated, enhanced sars-cov infection in the lungs. to explore this possibility, we examined whether co-infection of mice with sars-cov and pp, a low-pathogenic bacterium which elici ... | 2008 | 18380809 |
| upgrading mouse health and welfare: direct benefits of a large-scale rederivation programme. | we report the outcome of a 30-month programme to rederive 310 specific pathogen-free mouse strains to populate a new individually ventilated cage barrier facility at the mary lyon centre (mlc), medical research council (mrc) harwell. the mice were rederived in a self-contained quarantine suite and embryo-recipient females were health-screened to assess microbiological status, before moving their offspring into the new facility. the mlc currently houses approximately 49,000 mice in about 9750 cag ... | 2008 | 18435871 |
| contemporary prevalence of infectious agents in laboratory mice and rats. | periodic health screening of rodents used in research is necessary due to the consequences of unwanted infections. one determinant of the risk of infection for any given agent is its prevalence; other factors being equal, a prevalent agent is more likely than a rare one to be introduced to a research facility and result in infection. as an indicator of contemporary prevalence in laboratory populations of rats and mice, the rate of positive results in the samples received at a major commercial ro ... | 2009 | 19015179 |
| comparative analysis of pasteurella pneumotropica isolates from laboratory mice and rats. | selected biochemical and genetic characteristics of the wild-type strains of pasteurella pneumotropica isolated from mice and rats were investigated and compared in order to determine the significant differences among the isolates. the isolates were divided into six groups on the basis of the patterns of carbon source utilization in the host rodents. the genome sizes were determined by electrophoretic analysis, and the mean genome size of the isolates from mice was larger than that of the isolat ... | 2009 | 19234758 |
| flexible low-cost system for small animal aerosol inhalation exposure to drugs, proteins, inflammatory agents, and infectious agents. | the design for a simple, low-cost aerosol generation system for rodent inhalation studies is described here. this system is appropriate for low biohazard-level agents. in this study, two biosafety level 2 agents, pasturella pneumotropica and pseudomonas aeruginosa, were tested successfully. this system was also used to immunize mice and guinea pigs in ovalbumin-based models of pulmonary inflammation. this design is appropriate for studies with limited budgets and lower-level biosafety containmen ... | 2009 | 19317668 |
| identification and characterization of hemolysin-like proteins similar to rtx toxin in pasteurella pneumotropica. | pasteurella pneumotropica is an opportunistic pathogen that causes lethal pneumonia in immunodeficient rodents. the virulence factors of this bacterium remain unknown. in this study, we identified the genes encoding two rtx toxins, designated as pnxi and pnxii, from the genomic dna of p. pneumotropica atcc 35149 and characterized with respect to hemolysis. the pnxi operon was organized according to the manner in which the genes encoded the structural rtx toxin (pnxia), the type i secretion syste ... | 2009 | 19363112 |
| comparison of polymerase chain reaction primer sets for amplification of rodent pasteurellaceae. | monitoring of rodents for pasteurellaceae infection may be carried out by the polymerase chain reaction (pcr). we tested which of 17 rodent pasteurellaceae strains were detected by three pcr primer sets. by phylogenetic analysis, 12 strains were assigned to the rodent cluster and five strains to other clusters, namely the somnus cluster, pasteurella sensu stricto, actinobacillus sensu stricto, the mannheimia and rossii cluster. a primer set developed to detect biotype heyl [pasteurella] pneumotr ... | 2009 | 19505934 |
| a specific polymerase chain reaction based on the gyrb gene sequence and subsequent restriction fragment length polymorphism analysis of pasteurella pneumotropica isolates from laboratory mice. | 2009 | 19653941 | |
| an outbreak of pasteurella pneumotropica in genetically modified mice: treatment and elimination. | a closed breeding colony comprising genetically engineered, wild-type, and stock mice presented with varying degrees of bilateral mucopurulent conjunctivitis and panophthalmitis. the one mouse with unilateral corneal ulceration, a knockout animal, was submitted for necropsy, and bacterial culture samples were obtained from the affected eye and uterus. in addition, ocular swabs from another 12 clinically affected animals, consisting of knockout, transgenic, wild-type, and stock mice, were submitt ... | 2003 | 19757621 |
| dog bite wound infection by pasteurella dagmatis misidentified as pasteurella pneumotropica by automated system vitek 2. | 2009 | 19765934 | |
| enterobacteriaceae and pseudomonadaceae on the dorsum of the human tongue. | the aim of this study was to correlate the presence of enterobacteriaceae, pseudomonadaceae, moraxellaceae and xanthomonadaceae on the posterior dorsum of the human tongue with the presence of tongue coating, gender, age, smoking habit and denture use. | 2009 | 19936511 |
| suppurative adenitis of preputial glands associated with corynebacterium mastitidis infection in mice. | suppuration of the preputial gland in mice occurs as a septic complication of fight wounds around the external genitalia. currently reported bacterial isolates from these lesions are limited to staphylococcus aureus, pasteurella pneumotropica, and klebsiella oxytoca. in the context of a pilot experiment aimed at defining the aging phenotype of estrogen receptor beta knockout (berko) mice, 2 male mice (1 of the berko line and the other from the age- and sex-matched wild-type control group) were d ... | 2010 | 20122320 |
| perturbations in cytokine gene expression after inoculation of c57bl/6 mice with pasteurella pneumotropica. | pasteurella pneumotropica can cause inflammation and abscess formation in a variety of tissues. most commonly, p. pneumotropica produces clinical disease in immunodeficient mice or those concurrently infected with other pathogens. because clinical disease is infrequent in immunocompetent mice harboring p. pneumotropica, some scientists consider it an opportunistic pathogen with little clinical relevance to biomedical research. however, other infectious agents, including mouse parvoviruses, mouse ... | 2010 | 20158944 |
| identification of bacteria associated with feline chronic gingivostomatitis using culture-dependent and culture-independent methods. | feline chronic gingivostomatitis (fcgs) is a chronic inflammatory disease of the oral cavity that causes severe pain and distress. there are currently no specific treatment methods available and little is known regarding its aetiology, although bacteria are thought to play a major role. the purpose of this study was to identify the oral bacterial flora in normal and diseased cats. oral swabs were obtained from the palatoglossal folds of eight cats (three normal and five fcgs) and were subjected ... | 2011 | 20828946 |
| respiratory tract colonization by pasteurella pneumotropica in a patient with an alpha1-antitrypsin deficiency unexpectedly well identified by automated system vitek 2. | 2010 | 20846595 | |
| assessment of rpob and 16s rrna genes as targets for pcr-based identification of pasteurella pneumotropica. | diagnosis of pasteurella pneumotropica in laboratory animals relies on isolation of the organism, biochemical characterization, and, more recently, dna-based diagnostic methods. 16s rrna and rpob gene sequences were examined for development of a real-time pcr assay. partial sequencing of rpob (456 bp) and 16s rrna (1368 bp) of pasteurella pneumotropica isolates identified by microbiologic and biochemical assays indicated that either gene sequence can be used to distinguish p. pneumotropica from ... | 2010 | 21262128 |
| molecular and virulence characteristics of an outer membrane-associated rtx exoprotein in pasteurella pneumotropica. | pasteurella pneumotropica is a ubiquitous bacterium that is frequently isolated from laboratory rodents and causes various clinical symptoms in immunodeficient animals. currently two rtx toxins, pnxia and pnxiia, which are similar to hemolysin-like high-molecular-weight exoproteins are known in this species. in this study, we identified and analyzed a further rtx toxin named pnxiiia and the corresponding type i secretion system. | 2011 | 21410992 |
| infectious microorganisms in mice (mus musculus) purchased from commercial pet shops in germany. | in this study, we investigated the prevalence of infectious microorganisms (viruses, bacteria, fungi and eukaryotic parasites) in mice from different pet shops in germany; such animals may compromise the hygienic integrity of laboratory animal vivaria if private pet holders act as unintended vectors of infections carried by them. house mice sold as pets or feed specimens were purchased from different pet shops and tested for a comprehensive panel of unwanted microorganisms. we found a number of ... | 2011 | 21508117 |
| [epidural abscess caused by pasteurella pneumotropica.] | 2011 | 21684046 | |
| pathogenicity of pasteurella pneumotropica in immunodeficient nod/shijic-scid/jcl and immunocompetent crlj:cd1 (icr) mice. | pasteurella pneumotropica is an opportunistic pathogen in rodents. natural infection in immunodeficient animals suggests that immunodeficiency is a major factor in p. pneumotropica pathogenesis. to understand this process, we performed clinical, pathological and bacteriological studies of immunodeficient nod/shijic-scid/jcl and immunocompetent crlj:cd1 (icr) mice experimentally infected with p. pneumotropica atcc 35149. from 14 days postinoculation, some of p. pneumotropica-infected nod/shijic-s ... | 2011 | 22041283 |
| implementation of a pcr assay of pasteurella pneumotropica to accurately screen for contaminated laboratory mice. | the authors implemented a pcr protocol to rapidly screen for pasteurella pneumotropica and to accurately identify contaminated laboratory mice in a clinical setting. this protocol was implemented in response to a severe outbreak of p. pneumotropica in their animal facility. although a sentinel program was in place to routinely screen for p. pneumotropica, it was inadequate for the identification of contaminated animals. as a result, several additional strains of mice were contaminated and develo ... | 2011 | 22358207 |
| first reported case of pasteurella pneumotropica tricuspid valve endocarditis. | 2012 | 22521380 | |
| [development and application of taqman mgb probe fluorescence quantitative pcr method for rapid detection of clostridium piliforme]. | to develop a taqman mgb probe-based, sensitive and specific fluorescence quantitative pcr assay method for rapid detection of clostridium piliforme. | 2012 | 22575149 |
| analysis of 16s-23s rrna internal transcribed spacer regions in pasteurellaceae isolated from laboratory rodents. | the internal transcribed spacer (its) regions of members of pasteurellaceae isolated from rodents, including the [pasteurella] pneumotropica biotypes jawetz and heyl, [actinobacillus] muris, "hemophilus influenzaemurium" and bisgaard taxon 17 were studied and their feasibility to discriminate these species was analyzed. the reference strains of all species analyzed showed unique species-specific its patterns which were further present in 49 clinical isolates of [p.] pneumotropica biotypes jawetz ... | 2012 | 22771472 |
| current microbiological status of laboratory mice and rats in experimental facilities in japan. | on the basis of our 2011 microbiological monitoring tests, we report here the current microbiological status of mice and rats housed in experimental facilities in japan. we tested more than 14,000 mice, 6,000 serum samples, 500 fecal or cecal samples, and 200 lung samples from 3,549 mouse facilities within japanese universities and institutes (u/i), pharmaceutical companies and contract research organizations (p/c). we also tested more than 1,500 rats, 1,600 serum samples, and 20 fecal or cecal ... | 2013 | 23357945 |
| protective anti-outer membrane protein immunity against pasteurella pneumotropica infection of mice. | the ability of recombinant outer membrane proteins of pasteurella pneumotropica to vaccinate against the infections of mice was studied. the proteins examined were the homologues of the p4, p6, p26, and d15 proteins of haemophilus influenzae. intranasal vaccination with p4 and p6 produced protection against pneumonia. p6 vaccination, which was most studied, reduced the peak bacteria load in lungs by 50-fold and caused a rapid resolution of an infection that lasted for at least 5 days in unvaccin ... | 2013 | 23624107 |
| specific detection and identification of [actinobacillus] muris by pcr using primers targeting the 16s-23s rrna internal transcribed spacer regions. | [actinobacillus] muris represents along with [pasteurella] pneumotropica the most prevalent pasteurellaceae species isolated from the laboratory mouse. despite the biological and economic importance of pasteurellaceae in relation to experimental animals, no molecular based methods for the identification of [a.] muris are available. the aim of the present investigation was to develop a pcr method allowing detection and identification of [a.] muris. in this assay, a pasteurellaceae common forward ... | 2013 | 23665068 |
| development of a multiplex pcr assay based on the 16s-23s rrna internal transcribed spacer for the detection and identification of rodent pasteurellaceae. | the rodents pasteurellaceae have to be excluded from the specified pathogen free experimental animal facilities. despite the biological and economic importance of pasteurellaceae in relation to experimental animals just a few molecular based methods are available for their detection and identification. the aim of the present investigation was to develop a multiplex pcr assay allowing detection of all rodent pasteurellaceae and identification of [pasteurella] pneumotropica biotype jawetz, [p.] pn ... | 2013 | 24055385 |
| actinobacillus equuli ssp. haemolyticus in a semi-occlusively treated horse bite wound in a 2-year-old girl. | we report on the isolation of actinobacillus equuli ssp. haemolyticus from wound smears of a 2-year-old girl who was admitted to the hospital due to partial amputation of the distal phalanx of her right middle finger caused by a horse bite. a. equuli typically causes diseases in horses and only very few reports describing human infections (mostly associated with wounds) are available in the literature. interestingly, although the bacteria could be found in consecutive samples taken at different ... | 2013 | 24068980 |
| intranasal immunization with a non-adjuvanted adhesive protein descended from pasteurella pneumotropica and its preventive efficacy against opportunistic infection in mice. | intranasal vaccination is one of the most effective means of protecting against invading and colonizing pathogens because the vaccine elicits a mucosal immune response. the exploitation of vaccine adjuvants and delivery systems for intranasal vaccines is an important way to evoke antigen immunogenicity and elicit a better immune response at the mucosal sites. in the present study, we assessed the potential of intranasal immunization using a non-adjuvanted bacterial adhesive protein toward the ho ... | 2013 | 24091313 |
| efficacy of direct detection of pathogens in naturally infected mice by using a high-density pcr array. | we used a high-density array of real-time pcr assays for commonly reported rodent infectious agents (pria) to test naturally infected index mice and sentinel mice exposed by contact and soiled-bedding transfer. pria detected 14 pathogens--including viruses, bacteria, fur mites, pinworms, and enteric protozoa--in 97.2% of 28 pooled fecal samples, fur-perianal swabs, and oral swabs from 4 cages containing a total of 10 index mice. among these pathogens, pria (like conventional health monitoring me ... | 2013 | 24351765 |
| effect of weak acid hypochlorous solution on selected viruses and bacteria of laboratory rodents. | weak acid hypochlorous solution (wahs) is known to have efficacy for inactivating pathogens and to be relatively safe with respect to the live body. based on these advantages, many animal facilities have recently been introducing wahs for daily cleaning of animal houses. in this study, we determined the effect of wahs in inactivating specific pathogens of laboratory rodents and pathogens of opportunistic infection. wahs with an actual chloride concentration of 60 ppm and a ph value of 6.0 was ge ... | 2014 | 24770639 |
| growth ability of gram negative bacteria in free-living amoebae. | when bacteria and free-living amoebae (flas) live both in natural waters and man-made aquatic systems, they constantly interact with each other. some bacteria can survive and grow within flas. therefore, it has recently been thought that flas play an important role in spreading pathogenic bacteria in aquatic systems. in this study we investigated the intracellular growing ability of 7 different gram-negative bacteria (pseudomonas fluorescens, pseudomonas putida, pasteurella pneumotropica, aeromo ... | 2014 | 24955522 |
| draft genome sequence of the rodent opportunistic pathogen pasteurella pneumotropica atcc 35149t. | pasteurella pneumotropica is an opportunistic pathogen in rodents that is commonly isolated from upper respiratory tracts in laboratory rodents. here, we report the draft genome sequence of the p. pneumotropica type strain atcc 35149, which was first isolated and characterized as biotype jawetz. | 2014 | 25103762 |
| nasal adenocarcinoma and secondary chronic sinusitis in a hyacinth macaw (anodorhynchus hyacinthinus). | an adult male hyacinth macaw (anodorhynchus hyacinthinus) that presented for acute onset nasal discharge and dyspnea had purulent discharge from the right naris and serosanguineous discharge from the left naris on physical examination. results of a complete blood count revealed severe leukocytosis with a mature heterophilia. computed tomography scans showed a large amount of soft-tissue attenuating material within the infraorbital sinus and associated diverticula. aerobic culture results of the ... | 2014 | 25115043 |
| elimination of pasteurella pneumotropica from a mouse barrier facility by using a modified enrofloxacin treatment regimen. | multiple nod. cg-prkdc(scid)il2rg(tm1wjl)tg(hla-a2.1)enge/sz (nsg/a2) transgenic mice maintained in a mouse barrier facility were submitted for necropsy to determine the cause of facial alopecia, tachypnea, dyspnea, and sudden death. pneumonia and soft-tissue abscesses were observed, and pasteurella pneumotropica biotype jawetz was consistently isolated from the upper respiratory tract, lung, and abscesses. epidemiologic investigation within the facility revealed presence of this pathogen in mic ... | 2014 | 25255075 |
| pasteurella pneumotropica evades the human complement system by acquisition of the complement regulators factor h and c4bp. | pasteurella pneumotropica is an opportunist gram negative bacterium responsible for rodent pasteurellosis that affects upper respiratory, reproductive and digestive tracts of mammals. in animal care facilities the presence of p. pneumotropica causes severe to lethal infection in immunodeficient mice, being also a potential source for human contamination. indeed, occupational exposure is one of the main causes of human infection by p. pneumotropica. the clinical presentation of the disease includ ... | 2014 | 25347183 |
| microbiological survey of mice (mus musculus) purchased from commercial pet shops in kanagawa and tokyo, japan. | information regarding the prevalence of infectious agents in mice in pet shops in japan is scarce. this information is particularly useful for minimizing the risk of potential transmission of infections to laboratory mice. therefore, we surveyed infectious agents in mice from pet shops in kanagawa and tokyo, japan. the survey was conducted in 28 mice from 5 pet shops to screen for 47 items (17 viruses, 22 bacteria and fungi, 10 parasites) using culture tests, serology, pcr, and microscopy. the m ... | 2014 | 25502736 |
| conjunctival flora of clinically normal and diseased turtles and tortoises. | in captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises. | 2015 | 25889261 |
| studies of the binding of ficolin-2 and ficolin-3 from the complement lectin pathway to leptospira biflexa, pasteurella pneumotropica and diarrheagenic escherichia coli. | ficolins recognize pathogen associated molecular patterns and activate the lectin pathway of complement system. however, our knowledge regarding pathogen recognition of human ficolins is still limited. we therefore set out to explore and investigate the possible interactions of the two main serum ficolins, ficolin-2 and ficolin-3 with different gram-negative bacteria. we used recombinant ficolin molecules and normal human serum, which were detected with anti-ficolin monoclonal antibodies. in add ... | 2015 | 26074063 |
| neonatal pulmonary macrophage depletion coupled to defective mucus clearance increases susceptibility to pneumonia and alters pulmonary immune responses. | resident immune cells (e.g., macrophages [mφs]) and airway mucus clearance both contribute to a healthy lung environment. to investigate interactions between pulmonary mφ function and defective mucus clearance, a genetic model of lysozyme m (lysm) promoter-mediated mφ depletion was generated, characterized, and crossed with the sodium channel β subunit transgenic (scnn1b-tg) mouse model of defective mucus clearance. diphtheria toxin a-mediated depletion of lysm(+) pulmonary mφs in wild-type mice ... | 2016 | 26121027 |
| spontaneous bacterial and fungal infections in genetically engineered mice: is escherichia coli an emerging pathogen in laboratory mouse? | the impact of particular microbes on genetically engineered mice depends on the genotype and the environment. infections resulting in clinical disease have an obvious impact on animal welfare and experimentation. in this study, we investigated the bacterial and fungal aetiology of spontaneous clinical disease of infectious origin among the genetically engineered mice from our institution in relation to their genotype. a total of 63 mice belonging to 33 different mice strains, from severe immunod ... | 2015 | 26281439 |
| reclassification of actinobacillus muris as muribacter muris gen. nov., comb. nov. | to reinvestigate the taxonomy of [actinobacillus] muris, 474 strains, mainly from mice and rats, were characterized by phenotype and 130 strains selected for genotypic characterization by 16s rrna and partial rpob gene sequencing. the type strain was further investigated by whole-genome sequencing. phylogenetic analysis of the dna sequences showed one monophyletic group with intragroup similarities of 96.7 and 97.2 % for the 16s rrna and rpob genes, respectively. the highest 16s rrna gene sequen ... | 2015 | 26296776 |
| characterization of biofilm formation in [pasteurella] pneumotropica and [actinobacillus] muris isolates of mouse origin. | [pasteurella] pneumotropica biotypes jawetz and heyl and [actinobacillus] muris are the most prevalent pasteurellaceae species isolated from laboratory mouse. however, mechanisms contributing to their high prevalence such as the ability to form biofilms have not been studied yet. in the present investigation we analyze if these bacterial species can produce biofilms in vitro and investigate whether proteins, extracellular dna and polysaccharides are involved in the biofilm formation and structur ... | 2015 | 26430880 |
| high-resolution melting curve analysis for identification of pasteurellaceae species in experimental animal facilities. | pasteurellaceae are among the most prevalent bacterial pathogens isolated from mice housed in experimental animal facilities. reliable detection and differentiation of pasteurellaceae are essential for high-quality health monitoring. in this study, we combined a real-time pcr assay amplifying a variable region in the 16s rrna sequence with high-resolution melting curve analysis (hrm) to identify and differentiate among the commonly isolated species pasteurella pneumotropica biotypes "jawetz" and ... | 2015 | 26556281 |
| non-oral gram-negative facultative rods in chronic periodontitis microbiota. | the subgingival prevalence of gram-negative facultative rods not usually inhabiting or indigenous to the oral cavity (non-oral gnfr), as well as selected periodontal bacterial pathogens, were evaluated by culture in untreated and treated chronic periodontitis patients. | 2016 | 26835659 |
| canine adenovirus type 1 and pasteurella pneumotropica co‑infection in a puppy. | in 2008, a 2 months-old male german shepherd was presented with fever, depression, and evident organic wasting. the puppy died within 48 hours after the onset of clinical signs. a complete necropsy was performed. bacteriological examination of samples from the brain, lung, liver, spleen, and bone marrow tested positive for pasteurella pneumotropica. histopathology demonstrated inflammatory and vascular lesions in the central nervous system and internal organs. canine adenovirus type 1 nucleic ac ... | 2017 | 27033531 |
| identification of a virulence determinant that is conserved in the jawetz and heyl biotypes of [pasteurella] pneumotropica. | [pasteurella] pneumotropica is a ubiquitous bacterium frequently isolated from laboratory rodents. although this bacterium causes various diseases in immunosuppressed animals, little is known about major virulence factors and their roles in pathogenicity. to identify virulence factors, we sequenced the genome of [p.] pneumotropica biotype heyl strain atcc 12555, and compared the resulting non-contiguous draft genome sequence with the genome of biotype jawetz strain atcc 35149. among a large numb ... | 2016 | 27402782 |
| the genomic sequence of the oral pathobiont strain ni1060 reveals unique strategies for bacterial competition and pathogenicity. | strain ni1060 is an oral bacterium responsible for periodontitis in a murine ligature-induced disease model. to better understand its pathogenicity, we have determined the complete sequence of its 2,553,982 bp genome. although closely related to pasteurella pneumotropica, a pneumonia-associated rodent commensal based on its 16s rrna, the ni1060 genomic content suggests that they are different species thriving on different energy sources via alternative metabolic pathways. genomic and phylogeneti ... | 2016 | 27409077 |
| exhaust air dust monitoring is superior to soiled bedding sentinels for the detection of pasteurella pneumotropica in individually ventilated cage systems. | reliable detection of unwanted organisms is essential for meaningful health monitoring in experimental animal facilities. currently, most rodents are housed in ivc systems, which prevent the aerogenic transmission of pathogens between cages. typically soiled-bedding sentinels (sbs) exposed to soiled bedding collected from a population of animals within an ivc rack are tested as representatives, but infectious agents often go undetected due to inefficient transmission. pasteurellaceae are among t ... | 2016 | 27931316 |
| influence of rack design and disease prevalence on detection of rodent pathogens in exhaust debris samples from individually ventilated caging systems. | sampling of bedding debris within the exhaust systems of ventilated racks may be a mechanism for detecting murine pathogens in colony animals. this study examined the effectiveness of detecting pathogens by pcr analysis of exhaust debris samples collected from ventilated racks of 2 different rack designs, one with unfiltered air flow from within the cage to the air-exhaust pathway, and the other had a filter between the cage and the air-exhaust pathway. for 12 wk, racks were populated with eithe ... | 2016 | 27931317 |
| survival of bacteria of laboratory animal origin on cage bedding and inactivation by hydrogen peroxide vapour. | this study aims to determine the ability of laboratory animal bacteria to resist desiccation and inactivation by hydrogen peroxide vapour (hpv) on paper bedding pieces. bedding pieces were saturated with bacterial suspensions in water or 2% (w/v) bovine serum albumin (bsa) in water, and held in a mouse facility. viable counts showed variable survival rates over time for the bacterial species used ([pasteurella] pneumotropica, muribacter muris, pseudomonas aeruginosa, acinetobacter redioresistens ... | 2016 | 27932683 |
| dc-stamp is an osteoclast fusogen engaged in periodontal bone resorption. | dendritic cell-specific transmembrane protein (dc-stamp) plays a key role in the induction of osteoclast (oc) cell fusion, as well as dc-mediated immune regulation. while dc-stamp gene expression is upregulated in the gingival tissue with periodontitis, its pathophysiological roles in periodontitis remain unclear. to evaluate the effects of dc-stamp in periodontitis, anti-dc-stamp-monoclonal antibody (mab) was tested in a mouse model of ligature-induced periodontitis ( n = 6-7/group) where paste ... | 2017 | 28199142 |
| actinobacillus hominis osteomyelitis: first reported case in the english language medical literature. | actinobacillus hominis is currently a rarely reported pathogen. it has previously been associated with respiratory tract infections and bacteraemia in debilitated patients. however, under-reporting may occur due to misidentification by commonly used laboratory bacterial identification systems. this case is, to the best of our knowledge, the first reported case of a. hominis osteomyelitis in the english language medical literature. | 2016 | 28348754 |
| pathology of aging in nod scid gamma female mice. | in the past decade, nod.cg- prkdc(scid) il2rg(tm1wjl)/szj (nsg, nod scid gamma) mice have become a model of choice in several areas of biomedical research; however, comprehensive data on their spontaneous age-related pathology are not currently available in the literature. the prevalence of spontaneous morbidity affecting aged nsg female breeders enrolled in a parasitology study was documented with classification of neoplastic and non-neoplastic (inflammatory, metabolic, degenerative) lesions. m ... | 2017 | 28355107 |
| clonal outbreaks of [ pasteurella] pneumotropica biovar heyl in two mouse colonies. | the aim of this study was to document the pathogenic role of biovar heyl of [ pasteurella] pneumotropica in mouse colonies. fifty-three isolates associated with mastitis and orbital, cutaneous and vaginal abscesses as well as isolates from the nose and vagina of healthy mice were investigated. according to phenotypic characteristics and rpob sequencing, the isolates were identified as [ p.] pneumotropica biovar heyl. pulsed-field gel electrophoresis (pfge) revealed five closely related profiles ... | 2017 | 28358244 |
| rodentibacter gen. nov. including rodentibacter pneumotropicus comb. nov., rodentibacter heylii sp. nov., rodentibacter myodis sp. nov., rodentibacter ratti sp. nov., rodentibacter heidelbergensis sp. nov., rodentibacter trehalosifermentans sp. nov., rodentibacter rarus sp. nov., rodentibacter mrazii and two genomospecies. | rodentibacter gen. nov. is proposed based on isolation and phenotypic characterization of strains, predominantly from rodents. the strains showed 86 % or higher rpob gene sequence similarity and indicated a genus-level relationship within pasteurellaceae. the strains compared at 16s rrna gene sequence level showed 93.8 % or higher similarity, and their genus-level relationship within pasteurellaceae was confirmed by phenotypic analysis. the type species rodentibacter pneumotropicus comb. nov. is ... | 2017 | 28629498 |
| characterization of demodex musculi infestation, associated comorbidities, and topographic distribution in a mouse strain with defective adaptive immunity. | a colony of b6.cg-rag1tm1mom tyrp1b-w tg(tcra,tcrb)9rest (trp1/tcr) mice presented with ocular lesions and ulcerative dermatitis. histopathology, skin scrapes, and fur plucks confirmed the presence of demodex spp. in all clinically affected and subclinical trp1/tcr mice examined (n = 48). pasteurella pneumotropica and corynebacterium bovis, both opportunistic pathogens, were cultured from the ocular lesions and skin, respectively, and bacteria were observed microscopically in abscesses at variou ... | 2017 | 28830578 |
| revised taxonomy and nomenclature of rodent pasteurellaceae: implications for monitoring. | pasteurellosis is a well-recognized disease with similar pathology in all laboratory rodent species. pasteurella pneumotropica is the most frequently mentioned member of the pasteurellaceae isolated from mice and rats. numerous other pasteurellaceae taxa have been obtained from mice, rats, and other rodent species. recently, rodent pasteurellaceae have been submitted to comprehensive genetic and phenotypic (polyphasic) taxonomic studies. as a result they are now classed within six validly publis ... | 2018 | 29385897 |