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effect of guinea-pig conceptus and its secretions on endometrial prostaglandin output.the co-culture of day-15 guinea-pig conceptuses or day-15 pregnant guinea-pig endometrium with day-15 non-pregnant guinea-pig endometrium had no inhibitory effect on pgf2 alpha output from the non-pregnant endometrium. unpurified proteins secreted by the day-15 guinea-pig conceptuses, or these proteins purified by blue sepharose cl-6b and ion-exchange column chromatography also had no inhibitory effect on pgf2 alpha output from day-15 non-pregnant guinea-pig endometrium cultured in vitro. howeve ...19921492104
percutaneous placement of inferior vena cava filters.pulmonary embolism is a serious and difficult problem. many approaches for the prevention of recurrent pulmonary embolism have been tried. percutaneous placement of inferior vena cava filters is an easy, safe, available and well established procedure for the prevention of pulmonary embolism. the authors review the indications for use of ivc filters, and they review the main filters available in terms of ease of use, the physical characteristics, the technique of introduction, the efficacy and mo ...20041492602
isolation and characterization of a newly identified haemophilus species from cats: "haemophilus felis".a gram-negative coccobacillus was isolated from the lower respiratory tract of a cat with chronic obstructive pulmonary disease. the isolate required co2 and v factor for growth and was initially identified as haemophilus paraphrophilus on the basis of its nutritional requirements, colony morphology, and some biochemical tests. because of the host specificity of haemophilus species and discrepancies in catalase, oxidase, and hemolytic activities, additional testing was done. extensive biochemica ...19921500518
recent studies on regulation of gastric motility in turkeys.recent studies have been aimed at determining how gastroduodenal contractions are initiated and at regulation of the stomach during the intestinal phase of gastric regulation. intrinsic, myogenic, electrical slow waves, the principal regulators of motility in the mammalian gut, have not been found in the avian gut. instead, contractions appear to be initiated by a neurogenic pacemaker located between the gastric isthmus and the pylorus. pacemaker impulses travel over the myenteric plexus to the ...20061539009
protein kinase c activity affects glucose-induced oscillations in cytoplasmic free ca2+ in the pancreatic b-cell.acute stimulation of protein kinase c (pkc) inhibited glucose-induced slow oscillations in cytoplasmic free ca(2+)-concentration, [ca2+]i, in mouse pancreatic b-cells. in pkc-depleted cells glucose induced rapid transients in [ca2+]i, lasting for approximately 10 s, superimposed on the slow oscillations in [ca2+]i. it was demonstrated that the transients did not occur in the absence of extracellular ca2+. each transient typically was preceded by a slow increase in [ca2+]i, representing the risin ...19921592121
transforming growth factor beta induces myoblast differentiation in the presence of mitogens.transforming growth factor beta 1 (tgf-beta 1) added to l6e9 rat skeletal myoblasts in mitogen-rich medium induces a rapid decrease in c-myc expression and delays progression through the g1 phase of the cell cycle. this growth inhibitory response is followed by cell commitment to terminal differentiation with elevated expression of myogenin muscle determination genes, induction of muscle-specific proteins, and formation of multinucleated myotubes. these results suggest that tgf-beta 1 may act as ...19921594628
ventriculopleural shunting used as a temporary diversion.due to the limited absorptive capacity of the pleural cavity, infants and young children are not generally ideal candidates for ventriculopleural shunts. we report using chest cavities as alternate for temporary diversion of csf in a young child. venous access to the cervical region could not be utilized because of scarring from previous procedures, while peritoneal access was contraindicated due to repeated pseudocyst formation. pleural effusions were removed by thoracentesis when necessary, an ...20061595400
retinal vein occlusion.retinal vein occlusion is a common form of retinal vascular disease, especially in middle-aged and older individuals. the diagnosis is based on the funduscopic finding of retinal vein dilatation in association with retinal hemorrhages and cotton-wool spots. the pathology can involve the entire venous system or can be limited to a branch of the central retinal vein. retinal vein occlusion can be distinguished clinically from diabetic retinopathy and other retinal diseases. treatment for the acute ...20061595515
treatment of haemophilus aphrophilus endocarditis with ciprofloxacin.a patient with haemophilus aphrophilus endocarditis was successfully treated with ciprofloxacin. the response to treatment with cefotaxime and netilmicin for 12 days was poor but was satisfactory to a 6 weeks' course of ciprofloxacin.19921602151
sensitivity of actinobacillus actinomycetemcomitans and haemophilus aphrophilus to oxidative killing.we examined the killing of actinobacillus actinomycetemcomitans and haemophilus aphrophilus by oxygen metabolites generated by the xanthine-xanthine oxidase (x-xo) system. this system generates a mixture of oxidants, including superoxide radical, hydrogen peroxide, hydroxyl radical, and possibly singlet oxygen. differential sensitivity to the x-xo system was observed among strains of a. actinomycetemcomitans; notably, 2 catalase-deficient strains and 2 strains representative of serotypes b and c ...19911668250
identity of prostate specific antigen and the semen protein p30 purified by a rapid chromatography technique.previous investigators have reported the identity of prostate specific antigen (psa) and the semen protein p30, but data to support these claims have not been published. we report here the rapid and continuous purification of psa using a simple two column chromatography technique originally developed for purification of p30. using commercial antisera to psa and original antisera to p30 for detection, we show that the two glycoproteins have identical purification profiles by this technique which ...19701700160
increased [met]enkephalin and decreased substance p in spinal cord following thermal injury to one limb.thermal injury to one hind limb of rats was induced by immersion into water at 62 degrees c. both a mild (15 s) or severe (30 s) lesion caused inflammation of the limb when observed 24 h later; but at this time the animals used the injured limb when they walked. animals with a severe lesion of the injured limb subsequently withdrew it from use when walking. limb withdrawal did not occur following a mild lesion. at 24 h following the lesion, lumbar spinal cord levels of [met]enkephalin, as measur ...19901700332
application of multivariate analyses of enzymic data to classification of members of the actinobacillus-haemophilus-pasteurella group.outer membrane vesicles and fragments from actinobacillus actinomycetemcomitans, actinobacillus lignieresii, actinobacillus ureae, haemophilus aphrophilus, haemophilus paraphrophilus, haemophilus influenzae, haemophilus parainfluenzae, pasteurella haemolytica, and pasteurella multocida were isolated and examined semiquantitatively for 19 enzyme activities by using the api zym micromethod. the enzyme contents of vesicles and fragments were compared with the enzyme contents of whole cells of the s ...19921736960
dna-bending on ligand binding; change of dna persistence length.this paper simulates the helix-characteristic changes of apparent dna persistence length caused by randomly distributed helix bends as induced, e.g., by dna-bound ligand molecules. the parameters varied are the constant angle gamma of helix bending and the size alpha of the dna drug binding site, but also the degree of dna-ligand binding cooperativity and the helix-unwinding angle. if the size of the binding site is comparable with the helix pitch, the influence of phasing between helix bends an ...20011741965
[the surgical treatment of superficial thrombophlebitis]. 20021767155
gentamicin-collagen sponge for local applications. 10 cases of chronic osteomyelitis followed for 1 year.a bovine collagen sponge containing gentamicin was applied locally in 10 patients with chronic osteomyelitis who were then followed for 1 year postoperatively. no adverse effects or recurrence of infections was observed. the release of gentamicin from the sponge was more complete and rapid than has been reported with polymethyl methacrylate (pmma) beads. compared with previous reports on pmma beads, a much higher gentamicin concentration in the wound exudate can be expected after implantation of ...19911767654
recombinant human insulin-like growth factor i: effects in normal subjects and implications for use in patients. 19911767745
the biting rate of triatoma infestans in argentina.1. the daily proportion of fed individuals in a population of the reduviid bug triatoma infestans (klug), maintained under natural climatic conditions in experimental chicken houses in central argentina, was estimated from the proportion of bugs retaining colourless urine in the rectum. 2. from the estimates of feeding frequency throughout a 1 year period, it was shown that temperature has a dominant effect on biting rate, but density-dependent effects became apparent during the warmest months. ...19911768924
infective endocarditis with haemophilus aphrophilus. 19911794912
intracranial abscesses in odense hospital. survey of bacteriology, epidemiology, and treatment with antibiotics, 1963-1989.fifty-nine patients were operated or punctured in 60 incidents of brain abscess from 1963-1989, twice as many in men as in women. the number of cases tripled in 1980 to an incidence of 3.6 per million inhabitants per year, supposedly due to the advent of computerized tomography. simultaneously, the aetiology changed from staphylococci and gram negative rods to dominance of streptococci and haemophilus aphrophilus. apart from temporal abscesses, there was no correlation between localisation in th ...19911802627
cell surface hydrophobicity and electrokinetic potential of actinobacillus actinomycetemcomitans and haemophilus aphrophilus.laboratory strains and fresh isolates of actinobacillus actinomycetemcomitans and haemophilus aphrophilus were examined for cell surface hydrophobicity and for electrokinetic properties under different experimental conditions. fresh isolates of a. actinomycetemcomitans and h. aphrophilus were hydrophobic. laboratory strains of a. actinomycetemcomitans were 20-30% less hydrophobic than fresh isolates. no difference was observed between laboratory and fresh isolates of h. aphrophilus. the ph of th ...19911812465
peritoneal macrophage response: an in vivo model for the study of synthetic materials.this study was designed to validate an in vivo model in rat to study cell-implant interactions. pieces of dacron and goretex and polydimethylsiloxane (reference material) precoated or not were implanted in the peritoneal cavity. in some cases the peritoneal cells were stimulated before the implantation. macrophage phagocytosis and fibrinocoagulolytic activities were investigated in parallel with morphological studies 6 h after implantation. a graded cell response related to the different situati ...19911831674
peritoneal macrophage response: an in vivo model for the study of synthetic materials.this study was designed to validate an in vivo model in rat to study cell-implant interactions. pieces of dacron and goretex and polydimethylsiloxane (reference material) precoated or not were implanted in the peritoneal cavity. in some cases the peritoneal cells were stimulated before the implantation. macrophage phagocytosis and fibrinocoagulolytic activities were investigated in parallel with morphological studies 6 h after implantation. a graded cell response related to the different situati ...19911831674
use of synthetic oligonucleotide dna probes for the identification of different strains of fusobacterium nucleatum.the ability of three different oligonucleotide probes to identify different oral strains of fusobacterium nucleatum was tested. probes 119 and 214 were designed on the basis of known amino acid sequences from the n-terminal end of an outer membrane protein of f. nucleatum strain fev1. probe h 2.1, was a randomly cloned chromosomal dna fragment of 2.1 kbp. the specificities of the probes were examined by testing each of them against a panel of five other species of fusobacterium (six strains), or ...19911837056
application of quadferm+ for the identification of fastidious gram-positive and gram-negative bacilli.the 2-hr quadferm+ kit (qf) (analytab products, plainview, ny) was compared with conventional tube tests for the identification of the hacek bacteria (haemophilus aphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, kingella kingae), other haemophilus and kingella spp., capnocytophaga, corynbacterium, and moraxella spp. test results were identical for 296 comparisons with 74 isolates (74%). in the remaining 104 comparisons for 26 isolates, 50 reactions ...19911873978
[haemophilus aphrophilus--a rare endocarditis pathogen]. 19911921900
some unusual members of the family pasteurellaceae isolated from human sources--phenotypic features and genomic relationships.twelve human isolates exhibiting the properties of the family pasteurellaceae but phenotypically deviating from established species, or resembling species of animal origin that are only rarely reported to occur in human materials, were checked for their identities by dna-dna hybridization. the collection consisted of one strain of actinobacillus lignieresii, two strains of actinobacillus hominis (mannose-positive), two hitherto undescribed actinobacillus or actinobacillus-like species, [pasteure ...19911930571
[identification and typing of actinobacillus actinomycetemcomitans by restriction endonuclease analysis].the aim of the present study was to investigate the potential of restriction endonuclease analysis (rea) for the identifying and typing of actinobacillus actinomycetemcomitans (aa). bacterial chromosomal dna was extracted and digested with bamhi, hindiii, sal i and xho i respectively and separated by agarose gel electrophoresis. dna fragment patterns of aa and haemophilus aphrophilus differed strikingly from each ether. the patterns of aa fdc y4 and aa atcc 29523, belonging to two different sero ...19901965593
detection of mediastinitis after heart transplantation by gallium-67 scintigraphy.we report the findings of a patient with post-cardiac transplant mediastinitis detected by 67ga-citrate imaging. fever and leukocytosis were the first clinical signs suggesting infection. the usual diagnostic modalities, including ct and ultrasound, failed to identify the site of infection. a 67ga scan showed intense abnormal uptake behind the sternum. the site of uptake was shown by necropsy to be necrotic tissue involving cardiac sutures, pulmonary arteries, and the aorta due to infection with ...19912022995
haemophilus aphrophilus endophthalmitis associated with a filtering bleb. 19912025158
[determination of dna base composition and levels of dna-dna hybridization of actinobacillus actinomycetemcomitans].using the strains of actinobacillus actinomycetemcomitans (aa) and haemophilus aphrophilus (haph) in experiments, the mole percent quanine plus cytosine content (mol% g + c) and level of dna-dna hybridization (dna homology) were determined for the first time in oral microbiologic study in china. dna was extracted by marmur's method. the mol% g + c of dna was determined by thermal denaturation method (tm). dna-dna hybridization was carried out by de ley's optical procedure (initial renaturation r ...19912032476
clinical radiobiology of squamous cell carcinoma of the oropharynx.local tumor control is analyzed in a series of 181 patients treated with definitive megavoltage radiotherapy (rt) for histologically proven squamous cell carcinoma of the oropharynx. considerable variation in treatment time stemmed from the general use of a split-course technique in 49 patients treated from 1978 to 1985. incomplete follow-up, in those patients alive and well at the termination of the study or who have died from metastases or intercurrent disease before developing a local recurre ...20072045294
reduced incidence of bone metastases in irradiated areas after external radiation therapy of prostatic carcinoma.fourteen males, out of 380 patients, treated with radiation to the central pelvis and lumbar spine for poorly differentiated prostatic carcinoma were analyzed in retrospect. the dose of radiation to the bones of the target area was 5,000 cgy. the patients showed no signs of metastases at bone scintigraphy performed in connection with the treatment. in an average of 34 months after finishing radiotherapy, the patients developed metastases at bone scintigraphy. the pattern was similar in all patie ...20072045303
[bacterial spondylitis caused by haemophilus aphrophilus].four weeks after a slight scratch a 43-year-old man noted a severe back pain. physical examination gave suspicion of a bacterial spondylitis. from the biopsy of the involved vertebral body haemophilus aphrophilus was isolated under aerobic growth conditions.19912060962
ciprofloxacin treatment of haemophilus paraphrophilus brain abscess. 19912071920
outer membrane proteins of actinobacillus actinomycetemcomitans and haemophilus aphrophilus studied by sds-page and immunoblotting.this investigation characterized and compared outer membrane proteins (omp) of the closely related actinobacillus actinomycetemcomitans and haemophilus aphrophilus by means of sds-page patterns and reactions on immunoblots with rabbit antiserum against a. actinomycetemcomitans fdc y4. reactions with serum from a patient with papillon lefévre syndrome (pls), from whom periodontal wild strains of a. actinomycetemcomitans had been isolated, were also studied. omp were purified with selective solubi ...19902080070
[phototherapy with uv-b in vitiligo].we report on 14 patients suffering from extentise vitiligo, who underwent phototherapy with uvb. after 12 months of treatment, 8 (57.1%) of the patients showed repigmentation of more than 75%. especially the patients with facial lesions of the skin types v and vi achieved a nearly complete and cosmetically very satisfying repigmentation.20072080652
an in vitro method to study the adherence of bacteria to saliva-treated tooth enamel sections.an in vitro bacterial adherence assay which employed human tooth enamel sections precoated with saliva and an epifluorescent staining technique with acridine orange was developed. the assay was used to study the adherence properties of fresh and type strains of the following oral bacterial species: bacteroides gingivalis, bacteroides intermedius, capnocytophaga species, haemophilus aphrophilus, actinobacillus actinomycetemcomitans, peptostreptococcus species, veillonella species, actinomyces isr ...19902087341
[empyema caused by haemophilus aphrophilus with secondary leukemoid reaction]. 19902090900
[in aino's ward comprehensive care is put to the test. interview by elisabet forslind]. 20062095072
sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria.a filter paper spot test with 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid as a substrate was used to study the prevalence of sialidase activity among gram-negative anaerobic and capnophilic bacteria. a total of 567 isolates representing four genera of obligate anaerobes and four genera of capnophilic organisms was tested. sialidase activity was detected in 94% of 66 isolates from the bacteroides fragilis group, 98% of 66 b. bivius isolates, and all isolates of the following species ...19902108991
multivariate analysis of quantitative chemical and enzymic characterization data in classification of actinobacillus, haemophilus and pasteurella spp.chemotaxonomic data for strains of actinobacillus, haemophilus and pasteurella spp. were analysed using three multivariate statistical strategies: principal components, partial least squares discriminant, and soft independent modelling of class analogy. the species comprised actinobacillus actinomycetemcomitans. haemophilus aphrophilus, h. paraphrophilus, h. influenzae, pasteurella multocida, p. haemolytica and p. ureae. strains were characterized by cell sugar and fatty acid composition, lysis ...19902118166
monoclonal antibody-coated latex agglutination assay for identification of actinobacillus actinomycetemcomitans.three monoclonal antibodies (mabs) to lipopolysaccharide of actinobacillus actinomycetemcomitans strain y4 (serotype b) and eight mabs to a serotype b-specific polysaccharide antigen of strain y4 were obtained. latex particles sensitized with an mab to the y4 lipopolysaccharide produced a positive agglutination with whole cells of all three serotypes of a. actinomycetemcomitans, but not with haemophilus aphrophilus, haemophilus paraphrophilus, haemophilus influenzae, porphyromonas (bacteroides) ...19902124492
[isolation of haemophilus aphrophilus from a blood culture].the authors describe the method of isolation and identification and differential diagnosis from a morphologically, cultivation and biochemically close species h. (a.) actinomycemcomitans. they discuss the problem of the taxonomic classification of the two species.19902142621
neutron radiotherapy for high grade gliomas: the search for the elusive therapeutic window. 20072168359
interstitial irradiation of cerebral gliomas in childhood by permanently implanted 125-iodine--preliminary results.19 children with a deeply located cerebral glioma were treated with continuous interstitial irradiation (stereotactically implanted 125-iodine). the accumulated dose at the tumor surface ranged in the low grade glioma group (group a) from 55 to 100 gy and in the high grade glioma group (group b) from 50 to 65 gy. patients in group b additionally received a fractionated external beam irradiation (15-20 gy boost dose). tumor shrinkage as seen on ct-scans 6 months postoperatively could be achieved ...20072168501
preferential adsorption of human neutrophil myeloperoxidase isoform iii by oral bacteria.neutrophil myeloperoxidase (mpo) adsorbs to bacteria as a pre-requisite for killing by the mpo/hydrogen-peroxide/chloride system. three chromatographically distinct isoforms of mpo (mpo i, mpo ii, and mpo iii) have been isolated from human neutrophils. the purpose of this study was to determine whether oral bacteria--including actinobacillus actinomycetemcomitans, capnocytophaga sputigena, haemophilus aphrophilus, and eikenella corrodens--differentially adsorb the three major isoforms of mpo fro ...19902172341
comparison of the rim-h rapid identification kit with conventional tests for the identification of haemophilus spp.a commercially available system, the rim-h system (austin biological laboratories, austin, tex.), was evaluated for its ability to rapidly and accurately identify various haemophilus spp. a total of 110 clinical isolates were tested by both the rim and conventional identification procedures. the rim agreed with the standard identification for 100% of the haemophilus influenzae (76 of 76) and 92.0% of the haemophilus parainfluenzae (23 of 25) isolates tested. the identifications of haemophilus pa ...19902203817
haemophilus aphrophilus endocarditis.we report a case of haemophilus aphrophilus endocarditis involving mitral and tricupsid valves in a boy with congenital heart disease. he had received dental treatment without antibiotic cover. prolonged high-dose ampicillin with gentamicin was necessary for cure. short courses of ampicillin alone may not successfully treat h. aphrophilus.19902206842
inter- and intrafamilial similarities of rrna cistrons of the pasteurellaceae.we performed hybridizations between labeled rrnas from seven representative members of the family pasteurellaceae and from three other taxa on the one hand and dnas from 53 strains known or presumed to belong to the pasteurellaceae on the other hand. the members of the pasteurellaceae are most closely related to members of the enterobacteriaceae, the vibrionaceae, the aeromonadaceae, and the genus alteromonas. the family pasteurellaceae is very heterogeneous. there are at least seven rrna branch ...19902223605
identification of haemophilus aphrophilus and actinobacillus actinomycetemcomitans by dna-dna hybridization and genetic transformation.dna-dna hybridization was used to identify clinical isolates as haemophilus aphrophilus or actinobacillus actinomycetemcomitans. some of the isolates were naturally competent for genetic transformation and were also used as dna recipients for identification of other isolates. the results obtained by hybridization were supported by interstrain-to-intrastrain transformation ratios. distinction between the closely related species h. aphrophilus and a. actinomycetemcomitans was generally clear-cut b ...19902229383
[the immunomodulation of the natural killer activity of the splenocytes in c3ha mice during hepatoma 22a growth].a single injection of c3ha mice with various immunomodulators-ds-rna, thymogene (tm) and cyclophosphamide (cy)--performed one day before transplantation of syngeneic hepatoma 22a cells led to a decrease in the tumor growth rate. the most prominent effect was found following the cy treatment. the nk cell activity estimated per spleen of mice treated with ds-rna and tm was seen increased in comparison with the control mice not given the modulators. the rate of tumor growth was due probably to this ...20072238117
haemophilus aphrophilus isolated from blood.h. aphrophilus was isolated from 11 successively cultivated blood samples of a male (72) with a fatal atypical endocarditis. identification and differential diagnosis from actinobacillus (haemophilus) actinomycetemcomitans was done on the basis of phenotypic characters of the microorganism.19902248686
a comparative clinical trial of duodenal ulcer healing with two regimens of cimetidine: 800 mg once nightly and 400 mg twice daily.the efficacy and safety of two dosage regimens of cimetidine were compared in a single-blind study of 50 adults with endoscopically proven duodenal ulcers. patients were randomly allocated to receive 800 mg cimetidine taken once nightly or 400 mg cimetidine taken twice daily for 4 weeks. following 4 weeks' treatment patients again underwent endoscopy and, if healing was incomplete, they received a further 4 weeks' treatment. at 4 weeks, healing occurred in 21/25 (84%) patients on the once-daily ...20082257964
differentiation between actinobacillus (haemophilus) actinomycetemcomitans, haemophilus aphrophilus and haemophilus paraphrophilus by multilocus enzyme electrophoresis.genetic relationships among isolates assigned to actinobacillus actinomycetemcomitans, haemophilus aphrophilus and h. paraphrophilus were determined by analysis of electrophoretically demonstrable allelic variation in 14 structural genes encoding metabolic enzymes. among the 51 isolates analysed there were 25 electrophoretic types (ets), among which mean genetic diversity per locus was 0.753. cluster analysis of ets demonstrated one well-defined group of 11 ets representing solely the genotypes ...19902269878
multivariate analyses of carbohydrate data from lipopolysaccharides of actinobacillus (haemophilus) actinomycetemcomitans, haemophilus aphrophilus, and haemophilus paraphrophilus.the taxonomic distinction between actinobacillus (haemophilus) actinomycetemcomitans and haemophilus aphrophilus and the taxonomic distinction between h. aphrophilus and haemophilus paraphrophilus have been questioned. this study was done to determine whether multivariate statistical analyses of carbohydrate data from lipopolysaccharides could be used to distinguish between these closely related species. lipopolysaccharides were extracted with phenol-water and purified. carbohydrates were assess ...19902275855
mast cell activation in sterile bladder and prostate inflammation.sterile inflammation of the bladder has often been associated with interstitial cystitis (ic), a urologic condition of unknown etiology, predominantly affecting young and middle-aged females, for which no effective therapy is known. recent reports have indicated that ic is associated with an increased number of bladder mast cells. here we report the case of a middle-aged man with chronic sterile hematuria, dysuria and lower abdominal pain associated with a high number of bladder and prostate mas ...19902276845
species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria.oligodeoxynucleotide probes were developed for identification of the periodontal bacteria actinobacillus actinomycetemcomitans, bacteroides gingivalis, b. intermedius types i and ii, b. forsythus, eikenella corrodens, fusobacterium nucleatum, haemophilus aphrophilus, streptococcus intermedius, and wolinella recta. probes were designed by sequencing the 16s rrna for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. these probes were specific w ...19902312676
film panel cases society of pediatric radiology. haemophilus aphrophilus. 19902349027
synthetic oligodeoxynucleotide probes for the rapid detection of bacteria associated with human periodontitis.analysis of the subgingival microflora has recently implicated actinobacillus (haemophilus) actinomycetemcomitans and several black bacteroides species in the aetiology of juvenile, adult and rapidly progressing periodontitis. rapid bacteriological diagnosis has been hampered by the slow growth and fastidious nature of these bacteria. to construct diagnostic probes, dideoxy sequencing of the 16s rrna molecules from a. (h.) actinomycetemcomitans, haemophilus aphrophilus, bacteroides gingivalis, b ...19882469776
ignorance about listeria. 19892504402
[aortic and mitral endocarditis caused by haemophilus paraphrophilus with abscess of the aortic ring and cerebral embolism].we report the first case of aortic and mitral haemophilus paraphrophilus endocarditis complicated by abscess of the aortic annulus in a 30-year old man with post-rheumatic mitral regurgitation. we recall the peculiar clinical features and course of this bacterial endocarditis of uncommon origin. we insist, in particular, on the occurrence of cerebral embolism and on the two-dimensional echocardiographic diagnosis of an aortic annulus abscess confirmed at surgery. cure was obtained by aortic and ...19892512879
[the psychology of the tuberculosis patient in different historical periods and its repercussions in social life, art and customs]. 20082519493
vecuronium infusion requirements in pediatric patients during fentanyl-n2o-o2 anesthesia.eighty-one pediatric patients, ranging from neonates to adolescents, were studied during fentanyl-n2o-o2 anesthesia to determine for each of them the vecuronium infusion required to maintain 90-95% neuromuscular block (nmb). electromyographic monitoring with train-of-four stimuli was used. the steady infusion rate was 62 +/- 15 (sd) micrograms.kg-1.hr-1 in neonates and infants. this rate was 40% of that required by children 3 to 10 years old (154 +/- 49 micrograms.kg-1.hr-1; p less than 0.05). i ...20072562909
[tumors of the adrenal medulla: nosologic profile and diagnostic technics].a nosologic re-arrangement of medullo-adrenal neoplasms has been looked for in this article, with particular regard to their common relationship to the complex histogenic system descending from the neuroectoderma-neural crista. the morpho-biologic features of these clinical entities are also reviewed, as well as the up to date laboratory and imaging techniques, with a stress on the high sensibility and specificity levels afforded, e.g., for some of these tumours by selected radio-isotopic examin ...20082576573
[the treatment of vocal cord paralysis using collagen]. 20082586082
[distribution pattern and risk factors of lung cancer in qidong rural area].distribution pattern, epidemiologic trend and risk factors of lung cancer in rural area were studied and analysed based on the data of qidong cancer registry (1972-1986), of sampling survey of cigarette smoking rate among rural residents, and of consumption of cigarette and fuel in qidong. the results showed that: 1. lung cancer mortality had increased from 9.00/10(5) in 1972 to 20.03/10(5) in 1986 with annual increase rate of 5.88%; 2. lung cancer mortality in 1980s (1984-1986) was increased by ...20092620633
in vivo chemotaxis evoked by actinobacillus actinomycetemcomitans and haemophilus aphrophilus.the chemotaxis-evoking capacity of 5 actinobacillus actinomycetemcomitans and 5 haemophilus aphrophilus strains were studied in a tissue cage model in rabbits. a significant increase of the total number of polymorphonuclear leukocytes was induced in the tissue cage fluid by both viable and killed bacteria, reaching a maximum after 12-24 h. in parallel, the proportion of viable/non viable leukocytes increased. the leukocyte counts declined during the following 24-48 h in all chambers except in th ...19892628865
diabetes mellitus. control and complications. 20092648354
[haemophilus aphrophilus epidural abscess studied by nuclear magnetic resonance]. 19892660661
complete amino acid sequence of rat brain hexokinase, deduced from the cloned cdna, and proposed structure of a mammalian hexokinase.the complete amino acid sequence for the type i isozyme of hexokinase from rat brain has been deduced from the nucleotide sequence of cloned cdna. the nucleotide sequence of 91 bases in the 5' untranslated region as well as that of the entire 3' untranslated region preceding the poly(a) sequence have also been determined. the n- and c-terminal halves of brain hexokinase show extensive sequence similarity to each other and to yeast hexokinase. these results provide direct support for the proposal ...19892704734
[long-term implications of gastroduodenal resection in peptic ulcer]. 20072717017
selected low-cohesion variants of actinobacillus actinomycetemcomitans and haemophilus aphrophilus lack distinct antigens recognized by human antibodies.actinobacillus actinomycetemcomitans omz 346 a and haemophilus aphrophilus omz 384 a, isolated on a synthetic selective and differentiating agar, show the highly cohesive and wall adherent growth in liquid medium which is typical for all primary oral isolates of these species. from each of them a low cohesion variant, omz 346 f and omz 384 f, respectively, was obtained by selection for cells growing in suspension. screening of western blots of these four strains with several human sera revealed ...19892719526
differentiation of some species of neisseriaceae and other bacterial groups by dna-dna hybridization.dna-dna hybridization using total genomic dna probes may represent a way of differentiating between miscellaneous bacterial species. this was studied with type and reference strains of 20 species in moraxella, kingella, and other selected gram-negative groups. both radioactive and biotin labelling were employed. most of the species examined were easily distinguished, such as moraxella (branhamella) catarrhalis, m.(b.) ovis, m. atlantae, m. phenylpyruvica, m. osloensis, neisseria elongata, n. men ...19892730785
celiac plexus block following high-dose opiates for chronic noncancer pain in a four-year-old child.this is the first case report documenting the use of a neurolytic celiac plexus block for relieving chronic noncancer pain in a pediatric patient. the child was a 4-yr-old male with an unknown form of inflammatory bowel disease since 1 yr of age. chronic abdominal pain became a problem at 3 yr of age, following multiple bowel resections; continuous intravenous narcotic administration was implemented for pain control. the patient's pain became refractory to high-dose morphine administration (maxi ...20022732525
[advanced age and clinical staging in the prognostic evaluation of differentiated carcinoma of the thyroid gland].the prognostic significance of age in differentiated thyroid tumours is assessed via the analysis of 156 cases of differentiated thyroid carcinoma surgically treated in 1967-85. a statistical analysis was performed on a sample of 120 patients under observation since their operation. the results indicate that the negative influence of old age is due more to the higher incidence of advanced tumours among the elderly than to more aggressive behaviour by the tumour.20092733846
a smile for the möbius' syndrome patient.a major problem for the patient with möbius' syndrome is the inability to smile. with the development of vascularized muscle transplantation, the possibilities for improved facial animation were explored. muscle transplantation was carried out in 7 patients; innervation was provided by the hypoglossal nerve, the motor nerve to the masseter muscle, or in 1 patient a transfacial nerve graft. in this article the technical aspects of the procedure as well as the results are described in detail.20092735718
influence of food on relative bioavailability of fluoride in man from na2fpo3-containing tablets for the treatment of osteoporosis.aqueous solutions of naf and sodium monofluorophosphate (na2fpo3, mfp) and tablets containing mfp (caflu, tridin) were given as single oral doses to fasting healthy volunteers in an intra-individual crossover study. serum fluoride (f) levels and urinary f concentrations were measured using an f-ion-sensitive electrode; 8 h profiles of f concentrations in serum and 24 h urinary f output were determined. f availability and pharmacokinetic data were identical for all items. in a second group of exp ...19892737790
psychological and environmental determinants of relapse in crack cocaine smokers.the paper reviews approaches to relapse in the treatment of cocaine abusers. approaches reveal a common mechanism underlying relapse that involves drug craving, recall of euphoria, environmental cues, denial, myths of being able to sell or use drugs, and painful affect states necessitating use of a multifaceted clinical technique. empirical validation of a common mechanism underlying relapse establishes a typology of psychological and environmental determinants of relapse for crack cocaine smoke ...20082746717
metastatic sweat gland carcinoma: response to 5-fluorouracil infusion.a 78-year-old white male presented with metastatic sweat gland carcinoma involving regional lymph nodes, pleura, pericardium, and chest wall. a complete response of three months duration was achieved by using a 96-hour infusion of 5-fluorouracil. this is the first report of a complete response to systemic chemotherapy in metastatic sweat gland carcinoma.20092770311
[construction and testing of a strain gauge for measuring masticatory force].the main objective of this study was to establish and test a measuring system for monitoring occlusal forces and force cycles during mastication. a measuring module using strain gauge transducers was incorporated in the bridge-pontic of a three part test bridgework. a load cell was used intraorally for loading and calibration. the test bridges were incorporated in the patient's mouth and calibrated in vivo using variable loads. the recording system was a special intensifying and processing syste ...20092772595
chemical differences in lipopolysaccharides from actinobacillus (haemophilus) actinomycetemcomitans and haemophilus aphrophilus: clues to differences in periodontopathogenic potential and taxonomic distinction.while actinobacillus actinomycetemcomitans has been associated with rapidly progressive periodontal destruction in man, the closely related haemophilus aphrophilus has not been related to periodontal disease. this may be due to differences in composition and structure of the lipopolysaccharides (lps) of these dental-plaque bacteria, since lps probably exerts a series of detrimental effects on the periodontium. lps was prepared by the phenol-water procedure from the type strains of a. actinomycet ...19892777374
[intraoperative monitoring of cerebral function and carotid hemodynamics during carotid endarterectomy].the authors investigated the hemodynamics and monitored the cerebral function to perform the carotid endarterectomy (cea) safely. the hemodynamics were investigated by measurements of carotid arterial blood flow by an electromagnetic flow meter before and after cea. and a doppler flow meter applied directly to the carotid arteries to analyze the flow parameters such as peak frequencies (pf), mean frequencies (mf), mode frequencies (mo f) and % window. we used routinely our specially designed shu ...20092812257
identification of an additional sendai virus non-structural protein encoded by the p/c mrna.peptide antiserum and monoclonal antibodies have detected a previously unrecognized small non-structural protein in sendai virus-infected cells. this protein, designated x, appears to represent the c-terminal 95 amino acids of the p protein reading frame. the x protein appears to be almost as abundant as the p protein on a molar basis in vivo. no evidence of a precursor-product relationship between the x and p proteins could be found.19872821189
low-grade adenocarcinoma of the oral cavity. a comparison between the terminal duct and the papillary type.fifteen cases of a recently-described type of adenocarcinoma of salivary gland tissue are reviewed. lesions are variously designated polymorphous low-grade adenocarcinoma, low-grade papillary adenocarcinoma, terminal duct carcinoma and lobular carcinoma. tumours were divided into those with papillary structures (8 cases) and those without (7 cases), respectively low-grade papillary adenocarcinoma (lgpa) and terminal duct adenocarcinoma (tda). mean age of tda-cases was 59.1 years; of the lgpa-cas ...20102828432
high-performance liquid chromatographic separation and immunological characterization of soluble bovine viral diarrhea virus antigen.sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blot analysis of the protein extracts from bovine viral diarrhea virus (bvdv, singer strain) infected primary calf testicle cells (soluble antigen) showed the presence of four virus specific polypeptides of 105, 90, 84 and 67 kilodaltons (kd) the 84-kd being the most abundant. anion-exchange high-performance liquid chromatography (hplc) of soluble antigen separated the virus specific polypeptides in individual peaks while the ...19882832429
immune response of chickens inoculated with a recombinant avian leukosis virus.specific-pathogen-free embryos (18-day incubation) and hatched chicks were inoculated with a recombinant avian leukosis virus (alv) produced by recombinant dna techniques. enzyme-linked immunosorbent assays were used to measure the production of viral-protein-specific antibody and the viral protein, p27, in the serum at 2, 5, 8, 14, and 20 weeks of age. of the inoculated chickens surviving to 20 weeks, 64% produced viral-protein-specific antibodies and 42% transiently produced the viral protein, ...19882838018
a transcription factor from simian virus 40 chromosomes which activates the viral late promoter in vitro.we have characterized a transcription factor, obtained from simian virus 40 (sv40) chromosomes, which activates transcription from the sv40 late promoter in vitro. the late promoter-activating factor was distinct from sv40 t antigen as judged by its behavior on chromatography on hydroxylapatite; it was not recognized by anti-t antibodies, while t antigen itself was recognized. t antigen from sv40 chromosomes, on the other hand, abolished transcription in vitro from the early promoter. in dnase i ...19882845142
[a case of hepatocellular carcinoma with intratumoral gas formation detected by plain abdominal x-ray film]. 20102846919
[epidemiological news on infectious diseases]. 20102852294
neurohumoral vasoconstrictor systems in heart failure.by means of specific inhibitors of the renin-angiotensin system (captopril) and of the sympathetic nervous activity (prazosin) in dogs with congestive heart failure, and by using a specific antagonist of the pressor activity of arginine vasopressin in rats with heart failure, we studied the influence of these pressor hormone systems on peripheral vascular resistance and cardiac function. all three humoral vasoconstrictor systems were stimulated in heart failure. the experiments in dogs showed th ...19852864248
"morbakka", another cubomedusan.there is a large member of the cubomedusan family, which has not yet been formally identified, that is very similar to tamoya haplonema. it is present in indo-pacific tropical waters but is also present in colder australian waters. a case of severe envenomation in south queensland is discussed and its clinical and histological features are described. vinegar inactivates the undischarged nematocysts of this species and is recommended as the initial first-aid treatment.20052870414
"morbakka", another cubomedusan.there is a large member of the cubomedusan family, which has not yet been formally identified, that is very similar to tamoya haplonema. it is present in indo-pacific tropical waters but is also present in colder australian waters. a case of severe envenomation in south queensland is discussed and its clinical and histological features are described. vinegar inactivates the undischarged nematocysts of this species and is recommended as the initial first-aid treatment.20052870414
restriction enzyme maps of the macropodid herpesvirus 2 genome.the dna of macropodid herpesvirus 2 (dorcopsis wallaby herpesvirus) was analysed using restriction enzymes and molecular cloning techniques. sets of ecori and sali clones were prepared which represented approximately 85 per cent of the genome, and these clones were used to map the dna by double-digestion and hybridization experiments. data on uncloned regions were obtained by analysing fragments excised from agarose gels, and terminal fragments were identified by exonuclease iii digestion. the g ...19872889444
possible hemodynamic basis to urethane anesthesia-induced reductions in renal clearance.the hemodynamic basis of reported urethane anesthesia-induced reductions in the clearance of some renally eliminated compounds has been investigated in the laboratory rat. the use of urethane as an anesthetic in pharmacokinetic studies still persists, particularly in experiments of long duration. using a microsphere technique, the per cent distribution of cardiac output to the kidneys, in both ip urethane- and ip hypnorm/hypnovel-anesthetized animals was significantly (p less than 0.01) lower th ...19882903036
vertebral osteomyelitis due to haemophilus aphrophilus: treatment with ceftriaxone. 19892926178
overprediction and blocking in the mccollough aftereffect.the rescorla-wagner theory (rescorla & wagner, 1972) of associative learning offers specific predictions about the associative strength of cs-us pairs when two or more css are conditioned to the same us separately and subsequently paired in a compound with the same us. the magnitude of orientation-contingent color aftereffects (aes) was used as an index of associative strength in this study. the results of experiments using an "overprediction" (rescorla, 1970) and a "blocking" (kamin, 1969) para ...20022928072
a powerful method for the preparation of cdna libraries: isolation of cdna encoding a 100-kdal nucleolar protein.an easy and quick method to synthesize large cdna molecules and to clone them with very high efficiency in the expression vector lambda gt11 is described. the technique employs rnase h and escherichia coli dna ligase treatment during second-strand synthesis, followed by repair of the ds cdna extremities by s1 nuclease and polik (klenow fragment) treatment. this treatment allows efficient addition of suitable linkers and results in a 100-fold increase in the yield of cloned cdna, when compared wi ...19852996985
antimicrobial properties of hydrogen peroxide and sodium bicarbonate individually and in combination against selected oral, gram-negative, facultative bacteria.the topical application of hydrogen peroxide (h2o2) and sodium bicarbonate (nahco3), individually and in combination, has been used empirically in the treatment of periodontal diseases. in this study, we examined both minimum inhibitory concentrations (mic) and minimum bactericidal concentrations (mbc) of these disinfectants individually and in combination against selected facultative, gram-negative oral bacteria in a microtiter dilution assay. the bacteria studied included actinobacillus actino ...19863016051
zona glomerulosa cell responses to atrial natriuretic factor in genetically hypertensive rats.we examined whether abnormalities in target cell responsiveness to atrial natriuretic factor (anf), similar to those previously found in the kidney, could also be present in the zona glomerulosa cells of spontaneously hypertensive rats (shr) and salt-sensitive dahl rats (s rats). we found an attenuated aldosterone (aldo) response to angiotensin ii (ang ii) in zona glomerulosa cell suspensions isolated from hypertensive shr compared with those from wistar-kyoto (wky) rats, whereas cells derived f ...19863020999
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