Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| comparison of sheffield media with standard media for the isolation of haemophilus ducreyi. | isolation of haemophilus ducreyi is the only method for the definitive diagnosis of chancroid. culture on supplemented gonococcal base (gchgs) or on supplemented muller-hinton agar (mhhb) has yielded the best isolation rates. sheffield media is an alternative to standard media. we compared the isolation rate of h. ducreyi on gchgs and mhhb to that on the sheffield media with and without 5% horse blood. vancomycin (3 mg/l) was added to all media. of the 87 specimens cultured from patients with ge ... | 1989 | 2662437 |
| recent developments in sexually transmitted diseases: chancroid--epidemiology, diagnosis, and treatment. | the most important recent developments concerning chancroid have been in its epidemiology (a tremendous increase in the incidence of the disease in the united states over the past several years), diagnosis (better culture media), and treatment (several newer and older drugs that have been shown to be effective against chancroid in recent studies). | 1989 | 2667898 |
| [the etiopathogenesis of ulcus molle: current status of knowledge--approaches to future research]. | chancroid is a sexually transmitted disease characterized by genital ulceration. although the pathology of chancroid lesions has been well described, we still know relatively little about the mechanism by which the causative agent, the bacterium haemophilus ducreyi, produces disease. it is hoped that a better understanding of both the pathogenicity of h. ducreyi and the pathogenesis of chancroid will provide a rational basis for the development of improved strategies for the control of this dise ... | 1989 | 2668228 |
| [diagnosis and therapy of ulcus molle today. case report and review of the literature]. | chancroid, an ulcerous disease of the genitalia caused by haemophilus ducreyi, occurs rarely but regularly in germany. exact diagnosis is based on the clinical features and a direct smear, and in particular on cultivation of the organism, showing its unique macromorphological characteristics. the sensitivity of cultivation has increased due to the development of selective media for primary isolation. resistance problems during the last decade meant that a change to new therapeutic strategies was ... | 1989 | 2668230 |
| results of treatment with quinolones of sexually transmitted diseases in the far east: a brief summary. | the emergence of penicillinase-producing neisseria gonorrhoeae (ppng) strains in 1976 and the increasing frequency of chromosomally mediated resistance to penicillin has resulted in a decline in the effectiveness of penicillin for the treatment of gonorrhea. in hong kong, the emergence of bacterial resistance to kanamycin or tetracycline has also been observed. quinolones were first introduced in hong kong in 1985, and since then the percentage of ppng-related cases of gonorrhea has dropped dram ... | 1989 | 2672256 |
| laboratory survey of fluoroquinolone activity. | fluoroquinolones are active against a wide variety of bacteria. the antibacterial spectra of fluoroquinolones encompass staphylococci, bacillus species, and corynebacterium species implicated in infections of the immunocompromised host; enterobacteriaceae; most intestinal pathogens; and many gram-negative organisms commonly causing nosocomial infections. haemophilus influenzae, haemophilus ducreyi, neisseria gonorrhoeae, neisseria meningitidis, and branhamella catarrhalis are highly susceptible ... | 1989 | 2672262 |
| chancroid and granuloma inguinale. | haemophilus ducreyi is a fastidious pathogen that can be routinely cultured with the appropriate media and incubation environment. prostituted women appear to be the usual reservoir. in africa, chancroid is emerging as the major risk factor for acquisition of hiv-1 following heterosexual intercourse. despite the emergence of resistance to a number of antimicrobial agents, h. ducreyi remains susceptible to ceftriaxone, erythromycin, and ciprofloxacin. control and eradication of outbreaks of chanc ... | 1989 | 2676322 |
| lymphogranuloma venereum: 27 cases in paris. | twenty-seven men with laboratory-confirmed lymphogranuloma venereum (lgv) were identified among 211 patients tested for lgv or chancroid during a 6-y period. the patients with lgv ranged in age from 17 to 73 y; most were from countries other than france. twenty-five sought care because of inguinal adenopathy (with spontaneous draining fistulae in two patients) and two because of proctitis. chlamydia trachomatis was isolated from nine patients; all isolates were the lgv biovar as demonstrated by ... | 1989 | 2677162 |
| clinical efficacy of antimicrobial therapy in haemophilus ducreyi infections. | ulcerative genital disease in the united states is commonly due to herpes simplex or syphilis. until recently, chancroid, an infection caused by the gram-negative streptobacillus haemophilus ducreyi, was infrequently diagnosed. continuing immigration, however, has reintroduced the disease into this country, and urban outbreaks have been associated with contact with infected prostitutes. extensive resistance has made previous antimicrobial agents ineffective. evaluations of multiple antibiotics a ... | 1989 | 2679402 |
| characterization of a multiple antibiotic resistance plasmid from haemophilus ducreyi. | plasmid pls88 from a clinical isolate of haemophilus ducreyi encoded resistance determinants for sulfonamides and streptomycin related to those of rsf1010 and for kanamycin related to tn903 but lacked the inverted repeats of the transposon. its host range included haemophilus influenzae, actinobacillus pleuropneumoniae, and escherichia coli; and it was compatible with pdm2 and rsf1010. | 1989 | 2684012 |
| biology of haemophilus ducreyi. | the etiological agent of the sexually transmitted genital ulcer disease chancroid was first described in 1889 by auguste ducrey following repeated autoinoculation of purulent ulcer material from a series of patients. the organism was isolated on artificial media a decade later but has remained difficult to isolate consistently, resulting in controversy over its characteristics and role as the causative agent of chancroid. because of its fastidious growth requirements, including unknown component ... | 1989 | 2687678 |
| in vitro activity of ofloxacin against chlamydia trachomatis. | the activity of ofloxacin against chlamydia trachomatis isolates obtained from eight metropolitan areas in the united states has been evaluated. growth of c. trachomatis in first passage was completely inhibited by less than 1 microgram/ml of ofloxacin (all 27 isolates tested). the bactericidal effect (inability to pass in the absence of antibiotics) was less than or equal to 1 microgram for 26 of the 27 isolates. other studies have shown that less than 0.1 microgram/ml of ofloxacin is the minim ... | 1989 | 2690615 |
| rapid detection of haemophilus ducreyi in clinical and experimental infections using monoclonal antibody: a preliminary evaluation. | a monoclonal antibody raised against haemophilus ducreyi was tested for its sensitivity and specificity as an immunofluorescence (if) reagent using simulated vaginal smears containing h. ducreyi, smears taken from skin lesions of mice infected with h. ducreyi and patients from south africa, thailand and malaysia with clinically diagnosed chancroid. the if test was more sensitive than culture or gram staining in the simulated smears, theoretically detecting less than 4 organisms/sample. it detect ... | 1989 | 2693334 |
| production of monoclonal antibodies specific for haemophilus ducreyi: a screening method to discriminate specific and cross-reacting antibodies. | haemophilus ducreyi is the etiological agent of chancroid. the organism shares extensive immunological cross-reactivity with other haemophilus species. this presents substantial difficulties for the production of specific monoclonal antibodies (mabs). a competition elisa was devised for hybridoma screening which allowed the detection of h. ducreyi-specific antibody-producing hybridoma cultures during the initial screening process. with this screening method, seven mabs specific for h. ducreyi we ... | 1989 | 2787274 |
| [morphologic variants of genital ulcer caused by haemophilus ducreyi]. | the typical clinical picture, common variants and frequent complications of chancroid are presented in cases proven by culture. in general, the conventional view of the clinical spectrum of chancroid is substantiated. certain types such as "chancre mou volant," however, could not be found in the present material. their existence or association with haemophilus ducreyi remains speculative. | 1989 | 2788154 |
| penicillin-binding proteins of haemophilus ducreyi. | the penicillin-binding protein (pbp) profile of haemophilus ducreyi was determined by a whole-cell-labeling assay. only two major pbps, of molecular weights 90,000 (pbp 1) and 38,500 (pbp 2), were detected in six of eight strains studied. competition binding experiments and the attendant morphological effects suggested that pbp 1 was either a functional amalgamation or a lack of resolution of two proteins equivalent to pbps 1 and 3 of escherichia coli. | 1989 | 2788389 |
| dna probes for the identification of haemophilus ducreyi. | haemophilus ducreyi atcc 33922, a virulent, well-characterized strain, was used to construct a genomic library in a bacteriophage expression vector. three dna fragments were selected for use as probes on the basis of their ability to encode h. ducreyi-specific proteins, as demonstrated by reactivity with rabbit polyclonal antiserum. with dna-dna hybridization, the three probes, labeled with 32p, reacted strongly with 16 strains of h. ducreyi obtained from a variety of sources. thirty-seven other ... | 1989 | 2788660 |
| causes and treatment of sexually acquired genital ulceration in southern africa. | the cause of sexually acquired genital ulceration was studied in 240 consecutive migrant mineworkers attending a sexually transmitted disease clinic in carletonville, transvaal. chancroid proved to be the most common cause of genital ulcer disease, with haemophilus ducreyi being isolated from 164 patients (68%). in 60% of cases the disease was locally acquired and in the remaining 40%, the infection was acquired in lesotho, botswana, natal, transkei and malawi. syphilis was diagnosed in 62 patie ... | 1989 | 2799580 |
| plasmid-mediated tet m in haemophilus ducreyi. | a tetracycline-resistant haemophilus ducreyi strain isolated in the united states was shown to carry a 34-megadalton plasmid which hybridized with the 1.8-kilobase kpni-hindiii tet m probe. the complete tn916 transposon hybridized with five different bands from this plasmid, suggesting homology throughout the length of the transposon. | 1989 | 2817857 |
| chloramphenicol-resistant haemophilus ducreyi in southern africa. | 1988 | 2899766 | |
| susceptibility of haemophilus ducreyi to spectinomycin in vitro. | using an agar dilution technique with standardised inocula prepared by ultrasonication, the minimum inhibitory concentrations (mics) of spectinomycin were determined for 66 strains of haemophilus ducreyi, eight of which were beta lactamase producers. all strains were sensitive to concentrations of spectinomycin, which were well within the therapeutic ranges attained with normal dosage. the mic50 and mic90 were 8 mg/l, (range 0.007-16 mg/l). | 1986 | 2942458 |
| in vitro activities of u-63366, a spectinomycin analog; roxithromycin (ru 28965), a new macrolide antibiotic; and five quinolone derivatives against haemophilus ducreyi. | the in vitro activities of the new spectinomycin analog u-63366, the new macrolide roxithromycin (ru 28965), and five new quinolone derivatives (pefloxacin, rosoxacin, norfloxacin, ofloxacin, and ciprofloxacin) were studied against 23 multiresistant strains of haemophilus ducreyi (beta-lactamase producers) isolated in paris and were compared with the activities of tetracycline, minocycline, chloramphenicol, streptomycin, kanamycin, gentamicin, spectinomycin, erythromycin, and nalidixic acid. all ... | 1986 | 2946262 |
| a comparison of single-dose spectinomycin with five days of trimethoprim-sulfamethoxazole for the treatment of chancroid. | in a controlled trial 32 patients with ulcers culture-positive for haemophilus ducreyi were treated with a single 2-g dose of spectinomycin, and 20 patients were treated with a five-day course of trimethoprim-sulfamethoxazole (tmp-smz; 160/800 mg) twice daily. both regimens rapidly eradicated h. ducreyi from ulcers, with subsequent healing of ulcers and buboes. the cure rates 14 days after initiation of therapy were 94% for spectinomycin and 95% for tmp-smz. patients with h. ducreyi-negative ulc ... | 1987 | 2956702 |
| in vitro activity of ceftriaxone, cefetamet (ro 15-8074), ceftetrame (ro 19-5247; t-2588), and fleroxacin (ro 23-6240; am-833) versus neisseria gonorrhoeae and haemophilus ducreyi. | we examined 300 strains of neisseria gonorrhoeae and 100 strains of haemophilus ducreyi to determine their in vitro susceptibility to two new cephalosporins, cefetamet (ro 15-8074) and ceftetrame (ro 19-5247; t-2588), and a new fluroquinolone, fleroxacin (ro 23-6240; am-833). their activity was compared with that of ceftriaxone, penicillin, spectinomycin, tetracycline, and erythromycin. cefetamet, ceftetrame, and fleroxacin had excellent in vitro activity against both groups of microorganisms. b ... | 1987 | 2959199 |
| anaerobic bacteria and herpes simplex virus in genital ulceration. | of 91 patients with genital ulceration, herpes simplex virus was isolated from 52 (57%) and haemophilus ducreyi from 12 (13%); none had syphilis. the difference in incidence of other aerobes in patients and controls was not significant. anaerobes, predominantly bacteroides spp, were isolated from a large proportion (77%) of men and women patients with ulcers but from few control men. the most common anaerobic species were b asaccharolyticus and b ureolyticus, with fewer isolates of the melaninog ... | 1985 | 2984108 |
| an outbreak of chancroid in orange county, california: descriptive epidemiology and disease-control measures. | from may 1981 through february 1983, greater than 1,700 patients were examined for genital ulcers at the orange county special diseases clinic in santa ana, california. of these patients, 923 had either confirmed chancroid or genital ulcers of unknown etiology. haemophilus ducreyi was recovered from lesions or inguinal buboes of 271 patients. in the previous year, no cases of chancroid were reported in this community. men accounted for 266 (98%) of the confirmed cases; 95% of the men were hispan ... | 1985 | 2985711 |
| the microbial aetiology of genital ulcers in black men in durban, south africa. | the microbial aetiology of genital ulcers was assessed in 100 black men attending a sexually transmitted disease (std) clinic in durban, south africa. forty patients harboured haemophilus ducreyi, one hepes simplex virus, and one neisseria gonorrhoeae. syphilis was diagnosed in 44 patients on the basis of dark field microscopy or positive syphilis serology test results, or both. of these 44 patients, eight also harboured n ducreyi, one herpes simplex virus. lymphogranuloma venereum was diagnosed ... | 1985 | 2991120 |
| characterization of a haemophilus ducreyi mobilizing plasmid. | the oriv site of haemophilus ducreyi mobilizing plasmid phd147, determined by replication in escherichia coli pola, is located close to the orit site. the orit site, located by recombination-proficient and -deficient cells, and the oriv site map in a region of phd147 homologous to the beta-lactamase-specifying plasmids of h. ducreyi and neisseria gonorrhoeae. | 1986 | 3003036 |
| plasmid-mediated aminoglycoside phosphotransferases in haemophilus ducreyi. | three clinical isolates of haemophilus ducreyi, representing at least two subtypes, were shown to be resistant to streptomycin and kanamycin. they also produced a beta-lactamase and chloramphenicol acetyltransferase and were resistant to tetracycline. in the three strains the resistance to both aminoglycoside antibiotics was encoded by a plasmid of ca. 4.7 kilobases which apparently did not carry ampicillin, chloramphenicol, or tetracycline resistance genes, as determined after transfer to esche ... | 1985 | 3010843 |
| susceptibility of haemophilus ducreyi to ampicillin and sulbactam in vitro. | the mics of ampicillin, ampicillin plus sulbactam in equal proportions, and a range of ampicillin concentrations with a constant 0.5 micrograms/ml concentration of sulbactam were determined for 66 strains of haemophilus ducreyi by an agar dilution technique with standardized inocula prepared by ultrasonication. fifty-five strains were susceptible to ampicillin alone (mic range, 0.06 to 1 microgram/ml). the mics for the 11 resistant strains were (micrograms per milliliter): range, 8 to greater th ... | 1986 | 3015016 |
| purulent penile ulcers of patients in singapore. | in 80 patients with painful purulent penile ulcers who attended the outpatient service of middle road hospital in singapore, haemophilus ducreyi was isolated from 18 (22%), herpes simplex virus type 2 from nine (11%), and neisseria gonorrhoeae from eight (10%). primary pathogens were not isolated from 45 (57%) men. painful purulent penile ulcers were more common in uncircumcised men, and patients had often acquired the disease after sexual intercourse with prostitutes in singapore. | 1986 | 3015775 |
| origin of small beta-lactamase-specifying plasmids in haemophilus species and neisseria gonorrhoeae. | fifty-nine percent of unselected strains of haemophilus parainfluenzae were found to carry small, phenotypically cryptic plasmid dna species. using filter blot hybridization, we found several plasmids which were homologous to the small beta-lactamase-specifying plasmids pjb1 and pfa7, which were originally isolated from haemophilus ducreyi and neisseria gonorrhoeae, respectively. detailed filter hybridization studies combined with electron microscope heteroduplex analysis suggested that three cr ... | 1986 | 3020002 |
| transfer of plasmid-mediated ampicillin resistance from haemophilus to neisseria gonorrhoeae requires an intervening organism. | haemophilus species have been implicated as the source of plasmid-mediated ampicillin resistance in neisseria gonorrhoeae. previous attempts to transfer conjugally the resistance plasmids from haemophilus species to n. gonorrhoeae have met with limited success. using both biparental and triparental mating systems, it was found that transfer will occur if the commensal neisseria species, neisseria cinerea, is used as a transfer intermediate. this organism stably maintains resistance plasmids of h ... | 1986 | 3020721 |
| enzyme profile of haemophilus ducreyi strains isolated on different continents. | two hundred strains of haemophilus ducreyi, isolated in different parts of the world, were investigated using the api-zym system, which included 95 different substrates. all strains produced aminopeptidase against beta-naphthylamide derivatives of l-lysine, glycine, l-arginine, l-alanine, d-l-methionine, glycyl-glycine, glycyl-l-alanine and l-leucine. all strains also produced alkaline and acid phosphatase and phosphohydrolase. nearly all strains showed esterase activity against butyrate, valera ... | 1987 | 3032608 |
| clinical and microbiological evaluation of 46 episodes of genital ulceration. | in 44 consecutive patients 46 episodes of genital ulceration were studied. the presumptive clinical diagnosis was evaluated by extensive microbiological investigations. in 15 (33%) episodes the clinical diagnosis did not accord with the microbiological one. chancroidal lesions were most commonly found to have other microbiological aetiology. secondary invasion of treponemal or viral lesions by other bacterial species (mostly anaerobes or pyogenic cocci) or genital pyodermia were the cause of con ... | 1987 | 3034762 |
| antigenic analysis of haemophilus ducreyi by western blotting. | twenty-one strains of haemophilus ducreyi were analysed by western blotting using two antisera produced in mice. common antigens of molecular weights 58, 46, 41, 28, 22 and 16 kda were detected in all the strains. the antigens were protein in nature, since they could not be detected in whole-cell lysates which had been treated with proteinase k. the h. ducreyi strains showed antigenic cross-reactivity with strains of h. influenzae and h. parainfluenzae, but showed minimal or no cross-reactivity ... | 1988 | 3042435 |
| treating chancroid with enoxacin. | increasing resistance of haemophilus ducreyi to antimicrobials necessitates further trials of new antimicrobial agents for treating chancroid. enoxacin has excellent in vitro activity against h ducreyi, and a randomised clinical trial of three doses of enoxacin 400 mg at intervals of 12 hours compared with a single dose of trimethoprim/sulphametrole (tmp/smt) 640/3200 mg was therefore conducted. of 169 men enrolled in the study, 86 received enoxacin and 83 received tmp/smt. ulcers were improved ... | 1988 | 3044978 |
| the clinical use of macrolides. | macrolides are active against streptococcus pneumoniae, legionella spp. and mycoplasma pneumoniae, the main causes of community-acquired pneumonia they may therefore be used for the empirical treatment of community-acquired pneumonia, although emergent resistance in str. pneumoniae limits their use in some parts of the world. in patients with bronchitis the use of macrolides reduces the severity and duration of symptoms. macrolides have also been used successfully in the treatment of otitis medi ... | 1988 | 3053568 |
| the structure, pathogenicity and genetics of haemophilus ducreyi. | 1988 | 3053917 | |
| in vitro activity of a-56619 (difloxacin), a-56620, and other new quinolone antimicrobial agents against genital pathogens. | the in vitro activities of two new carboxyquinolones, a-56619 (difloxacin) and a-56620, were compared with those of ciprofloxacin, norfloxacin, and ofloxacin against genital tract pathogens. all the quinolones were highly active against neisseria gonorrhoeae. a-56619 had the lowest mics against chlamydia trachomatis (mic range, 0.125 to 0.25 micrograms/ml) and haemophilus ducreyi (mic for 90% of isolates tested, 0.1 micrograms/ml). | 1986 | 3101590 |
| in vitro activity of azithromycin and erythromycin against organisms associated with bacterial vaginosis and chancroid. | the in vitro activity of the new macrolide azithromycin and erythromycin against haemophilus ducreyi, gardnerella vaginalis, bacteroides spp. and mobiluncus spp. was compared. gardnerella vaginalis, mobiluncus spp. and haemophilus ducreyi were highly susceptible to both agents but azithromycin was more effective overall against all three groups of organisms. the activity of both antibiotics against bacteroides spp. varied greatly, but azithromycin was again more active. collectively, 62% of all ... | 1988 | 3141171 |
| lectin typing of haemophilus ducreyi. | the cell wall carbohydrates of 43 strains of haemophilus ducreyi isolated in different parts of the world were subjected to lectin analysis using commercial panels containing 14 different plant lectins of known specificity. preliminary evidence indicated both intrastrain and inter-strain variation in cell wall carbohydrate composition. in addition, it was possible to group strains from different geographical areas by lectin agglutination patterns. lectin typing might thus become a useful marker ... | 1988 | 3143583 |
| urethral infection with haemophilus ducreyi in men. | carriage of haemophilus ducreyi has not been adequately investigated in men, and its role in urethritis is unknown. in this study 456 men with h. ducreyi-culture positive genital ulcers were investigated. among these men, 16 (3.5%) had urethritis. h. ducreyi was isolated from the urethra of nine men (1.9%). of 57 men who had chancroid but not urethritis, none had h. ducreyi isolated from the urethra. of 106 patients presenting with only acute urethritis, h. ducreyi was found in one. we conclude ... | 1988 | 3258676 |
| immunoglobulin m and g antibody response in rabbits after experimental haemophilus ducreyi infection. | the enzyme-linked immunosorbent assay and immunoblot technique were used to investigate the humoral immune response following intradermal infection of rabbits with haemophilus ducreyi. basically, the kinetics of igm and igg antibody synthesis was the same as in other bacterial infections. however, antibody titres in the enzyme-linked immunosorbent assay were remarkably low. in the immunoblot experiments, antibodies of both immunoglobulin classes were mainly directed against haemophilus ducreyi a ... | 1988 | 3260705 |
| antimicrobial susceptibilities of southern african isolates of haemophilus ducreyi. | we determined the antimicrobial susceptibilities of 122 recent clinical isolates of haemophilus ducreyi to 24 antimicrobial agents. all isolates produced beta-lactamase and were resistant to penicillins. the majority of strains were also resistant to tetracycline, doxycycline, and sulfamethoxazole. all isolates were susceptible to macrolides, quinolones, extended-spectrum cephalosporins, spectinomycin, rifampin, and amoxycillin-clavulanate. reduced susceptibility to minocycline, co-trimoxazole, ... | 1988 | 3264133 |
| antibiotic susceptibilities and plasmid profiles of haemophilus ducreyi isolates from southern africa. | thirty-eight strains of haemophilus ducreyi isolated in southern africa were tested in vitro against 15 antimicrobial agents including those frequently used for the treatment of chancroid. in addition, newer compounds which possess characteristics consistent with their possible use as single-dose therapy for the disease were tested. all isolates were found to be resistant to penicillin as a result of beta-lactamase production. the majority of strains were also resistant to tetracycline, sulphame ... | 1988 | 3264554 |
| laboratory diagnosis of chancroid. | 1988 | 3266100 | |
| use of an enzyme immunoassay to detect serum igg antibodies to haemophilus ducreyi. | an experimental enzyme immunoassay (eia) for detecting serum igg antibody to haemophilus ducreyi was developed using an ultrasonicated whole-cell antigen. the mean optical densities (od) for sera from men with proven chancroid from nairobi (47 patients) and bangkok (72 patients) were significantly higher than those obtained from nairobi men with genital ulcers not due to h. ducreyi, from nairobi men with urethritis, from pregnant women in nairobi, and from european men with sexually transmitted ... | 1988 | 3283255 |
| efficacy and safety of a single dose therapy of a 500 mg ciprofloxacin tablet in chancroid patients. | a single dose therapy of 500 mg ciprofloxacin was sufficient to cure 15 of 18 chancroid patients within six days and the remaining three within 7, 11 and 20 days respectively. the drug was well tolerated, and no serious side effects were observed. the mics of ciprofloxacin for the isolated haemophilus ducreyi strains ranged from 0.0015 to 0.02 mg/l (mean 0.01 mg/l). it may therefore be concluded from our study that the oral administration of ciprofloxacin to chancroid patients with or without bu ... | 1988 | 3286513 |
| evaluation of 500- and 1,000-mg doses of ciprofloxacin for the treatment of chancroid. | a randomized, double-blind study was performed comparing ciprofloxacin in a 500-mg single dose with 1,000 mg (500-mg doses given 12 h apart) for the treatment of chancroid in thailand. haemophilus ducreyi was isolated from 87 (48%) of 180 men with a clinical diagnosis of chancroid. for men with ulcers that were culture positive for h. ducreyi, rates of cure were 100% in the 500-mg group and 98% in the 1,000-mg group. for men with ulcers that were culture negative for h. ducreyi, rates of cure we ... | 1988 | 3293526 |
| [diagnosis and therapy of ulcus molle]. | in developing countries of tropical africa and south-east asia chancroid is the most important cause of genital ulzerations. in the last decade also in industrial countries of the western hemisphere sporadic epidemics of this sexually transmitted disease were observed. this surprising revival of a disease already thought to have died out induced advances in cultivation and characterization of the etiologic agent as well as new therapeutic approaches. treatment regimens based on erythromycin, cep ... | 1987 | 3318086 |
| a review of the in-vitro activity of roxithromycin against genital pathogens. | the in-vitro activity of roxithromycin against neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, treponema pallidum, gardnerella vaginalis and haemophilus ducreyi is reviewed. roxithromycin demonstrated equivalent activity to erythromycin against n. gonorrhoeae, c. trachomatis, m. hominis, u. urealyticum, g. vaginalis and h. ducreyi. in a rabbit model for syphilis, potentially useful activity against t. pallidum has been demonstrated. | 1987 | 3323169 |
| chancroid--a review. | chancroid is a sexually transmitted disease caused by haemophilus ducreyi. the infection is reported to be more common in the tropics. isolation of h. ducreyi is difficult even in the best of hands. the diagnosis of chancroid is often clinical. most chancroidal ulcers are polymicrobial in nature and this has resulted in conflicting reports of the efficacy of antibiotics used in the treatment of chancroid. in-vitro testing of clinical isolates indicated a wide variation in the prevalence of resis ... | 1987 | 3328556 |
| sexually transmitted diseases. genital ulceration with regional adenopathy. | the specific agent causing genital ulceration is difficult to identify without laboratory confirmatory testing including darkfield microscopy, syphilis and lymphogranuloma venereum serology, and culture for haemophilus ducreyi and herpes simplex virus. empiric antimicrobial therapy risks masking clinical or serologic diagnosis of syphilis. | 1987 | 3332784 |
| activity of pefloxacin and thirteen other antimicrobial agents in vitro against isolates from hospital and genitourinary infections. | the in-vitro activity of the quinolone derivative pefloxacin was compared with that of three other quinolones, five beta-lactam antibiotics and three aminoglycosides against 367 isolates from hospital patients and from out-patients with genitourinary infections. mic90 of pefloxacin and norfloxacin for each strain was the same; that of ciprofloxacin was a little lower. all strains except escherichia coli were resistant to nalidixic acid. pefloxacin was highly active against staphylococcus aureus ... | 1986 | 3460983 |
| chancroid. recent advances in treatment and control. | 1986 | 3485081 | |
| laboratory diagnosis of haemophilus ducreyi: sensitivity of culture media. | laboratory confirmation of the clinical diagnosis of chancroid requires the isolation of haemophilus ducreyi. enriched gonococcal (gchbs) and mueller-hinton agar (mhhb) both support the growth of most strains of h. ducreyi. in this study we compared the isolation rate of h. ducreyi on gchbs and mhhb media in 111 men with genital ulcer disease. a second culture was obtained in 84 men at 48 hr in order to determine the reproducibility of h. ducreyi culture. the sensitivity of a single and a sequen ... | 1986 | 3485507 |
| isolation and identification of haemophilus ducreyi in a clinical laboratory. | routine procedures used to isolate haemophilus ducreyi in a busy laboratory are reported. identification was based on colony morphology and nutritional and biochemical properties of 120 fresh isolates of h. ducreyi. these isolates grew very well on gonococcal agar and mueller-hinton agar incubated at 34 degrees c in candle extinction jars containing moistened filter paper. colonies varied in size, giving a polymorphic appearance. they were smooth, dome-shaped, and buff-yellow to grey in colour, ... | 1986 | 3489097 |
| clinical evaluation of rosoxacin for the treatment of chancroid. | one hundred seven men with haemophilus ducreyi-positive chancroid were assigned to receive 300 mg of rosoxacin as a single dose or 150 mg twice daily for 3 days. ulcers and buboes were followed clinically and bacteriologically for 1 month. of 40 evaluable males on the 3-day regimen, 38 (95%) were cured, while only 14 of 23 (61%) males on the single-dose regimen were cured; this regimen was discontinued. there was one ulcer relapse at day 21 in both groups; the one relapse in the single-dose grou ... | 1986 | 3489439 |
| immunoblot analysis of antigens associated with haemophilus ducreyi using serum from immunised rabbits. | sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting were used to characterise isolates of haemophilus ducreyi. isolates of h ducreyi were heterogeneous in protein composition, but isolates from single outbreaks appeared similar both in protein profiles and antigenic analysis. rabbits immunised with h ducreyi responded with a vigorous humoral immune response in which multiple antigenic polypeptides were detected. the most prominent antigens had molecular masses of 67, 42 ... | 1986 | 3490427 |
| field testing of modified bieling media for the isolation of haemophilus ducreyi in kenya. | 1986 | 3492376 | |
| haemophilus ducreyi infection in south florida: a rare disease on the rise? | increased numbers of patients with genital ulcers sought medical attention in the palm beach county, florida, sexually transmitted disease clinics from aug 1, 1982 to aug 31, 1983. we established that a small proportion of subsequent cases of genital ulcers were caused by haemophilus ducreyi, the etiologic agent associated with chancroid. we also set up a surveillance system to monitor this sexually transmitted infection, and have illuminated several recent cases of chancroid occurring in that a ... | 1987 | 3492766 |
| a clinico-bacteriological study of "chancroidal" ulcers treated at aiims hospital, new delhi. | 1986 | 3493217 | |
| relationship between lipopolysaccharide composition and virulence of haemophilus ducreyi. | the relationship between lipopolysaccharide (lps) composition and virulence of haemophilus ducreyi strains was investigated. glycoses identified in lps by gas-liquid chromatography were glucose, galactose, and their amino derivatives glucosamine and galactosamine. fucose was found in trace amounts but mannose and rhamnose, characteristic of the o-side chain of lps in many species, were not detected. qualitatively, the lps composition of the eight strains examined was similar and differences were ... | 1987 | 3494128 |
| etiology of nonvesicular genital ulcers in winnipeg. | twenty-eight patients with recent onset of nonvesicular ulcerative genital lesions were studied prospectively to define the microbial etiology. causative agents identified included herpes simplex virus (hsv), chlamydia trachomatis, haemophilus ducreyi, and treponema pallidum. three women and 25 men (seven of whom were homosexual) were studied. ten patients did not have an etiology identified. nine patients had syphilis, six had genital herpes, one had chancroid, one had granuloma inguinale, and ... | 1987 | 3494322 |
| comparison of antimicrobial susceptibility patterns of fifty-seven strains of haemophilus ducreyi isolated in amsterdam from 1978 to 1985. | the in-vitro activities of 16 antimicrobial agents were tested against 57 strains of haemophilus ducreyi isolated in amsterdam during an eight year period. the susceptibility patterns of the isolates from different years were compared. in the first four years more than 30% were beta-lactamase negative and showed mics for tetracycline of 2 mg/l or less. from 1982 to 1985 all strains, except one, produced beta-lactamase and were tetracycline resistant. furthermore, mics for cefotaxime, sulphametho ... | 1987 | 3494720 |
| selenium and the growth of haemophilus ducreyi. | one of the growth media in current use for haemophilus ducreyi comprises mueller hinton agar, chocolatised horse blood, serum and isovitalex (bbl). for a better understanding of growth factors, attempts were made to simplify this complex medium. the horse blood was replaced by haemin (200 micrograms/ml), the serum by albumin (0.2%), and isovitalex was substituted only by l-glutamine 0.01%. most of the strains grew, but when selenium ions were added in a concentration of 3.25 x 10(-3) micrograms/ ... | 1987 | 3500188 |
| the treatment of chancroid. | since the treatment of chancroid was reviewed in 1982, the results of subsequent treatment trials have offered the clinician additional therapeutic choices as well as shorter courses of therapy. erythromycin (500 mg four times a day for seven days) provides consistently effective treatment for cases acquired throughout the world, although erythromycin-resistant strains have been isolated in singapore. sulfamethoxazole and trimethoprim (800 mg/160 mg orally twice a day for seven days), ceftriaxon ... | 1986 | 3512872 |
| use of dot-immunobinding and immunofluorescence assays to investigate clinically suspected cases of chancroid. | in 1984 and 1985, outbreaks of genital ulcers occurred in florida and new york. initial investigations for syphilis, herpes simplex, chlamydia trachomatis, and haemophilus ducreyi did not implicate any of these organisms as etiologic agents. from the results of dot-immunobinding assays, we found that sera from the patients had higher levels of igm (30 [47.6%] of 63 patients) and igg (22 [34.9%] of 63 patients) reactivities with an outer-membrane preparation from h. ducreyi strain cip542 than wit ... | 1986 | 3517183 |
| laboratory diagnosis of chancroid in nairobi, kenya. | 1986 | 3533507 | |
| single-dose ceftriaxone for chancroid. | men with genital ulcers that were culture positive for haemophilus ducreyi were treated with intramuscular ceftriaxone and randomized to three different dose regimens. all but 1 of 50 men treated with 1 g of intramuscular ceftriaxone were cured. similarly, 0.5 and 0.25 g cured 43 of 44 men and 37 of 38 men, respectively. a single dose of 250 mg of intramuscular ceftriaxone is an effective treatment for chancroid. | 1987 | 3566241 |
| single-dose therapy with trimethoprim-sulfametrole for chancroid in females. | women with genital ulcer disease who were culture-positive for haemophilus ducreyi were treated with a single dose of the drug combination trimethoprim-sulfametrole; the dosage was 640 mg of trimethoprim and 3,200 mg of sulfametrole. all 27 women who were treated with this regimen and adequately followed were cured. thus a single dose of trimethoprim-sulfametrole appears to be an effective treatment regimen for women with chancroid in kenya. | 1986 | 3764627 |
| antimicrobial susceptibility and characterization of outer membrane proteins of haemophilus ducreyi isolated in thailand. | one hundred strains of haemophilus ducreyi isolated in thailand from patients with chancroid were tested by the agar dilution method against 10 antimicrobial agents and typed by outer membrane protein pattern by using sodium dodecyl sulfate gel electrophoresis. all strains produced beta-lactamase and were resistant to tetracycline, kanamycin, and sulfonamides. most had a decreased susceptibility to trimethoprim (mic for 50% of the strains [mic50], 0.5 micrograms/ml) and chloramphenicol (mic50, 8 ... | 1985 | 3872313 |
| chancroid and haemophilus ducreyi. | 1985 | 3872617 | |
| trimethoprim sulphamoxole in the treatment of chancroid. comparison of two single dose treatment regimens with a five day regimen. | in a prospective blinded study, 135 men with genital ulcers culture positive for haemophilus ducreyi, were randomized to one of three regimens. two single dose regimens, either the combination of sulphamoxole 3200 mg/trimethoprim 640 mg or trimethoprim 700 mg alone were compared to a five day regimen of sulphamoxole 800 mg/trimethoprim 160 mg twice daily. all 31 treated with a five day regimen of trimethoprim sulphamoxole healed without further treatment. of 27 patients treated with the single d ... | 1985 | 3876325 |
| comparative study of ceftriaxone and trimethoprim-sulfamethoxazole for the treatment of chancroid in thailand. | a single dose of ceftriaxone (250 mg) administered intramuscularly was compared with trimethoprim-sulfamethoxazole (tmp-smz; 160/800 mg) administered orally twice daily for seven days or with a single dose of tmp-smz (640/3,200 mg) administered orally for the treatment of chancroid in men in thailand. haemophilus ducreyi was isolated from 79 (48%) of 164 men with a clinical diagnosis of chancroid. for men with ulcers that were culture positive for h. ducreyi, rates of cure were 100% in 25 men tr ... | 1985 | 3876396 |
| [ulcus molle: clinical, diagnostic and therapeutic aspects]. | 1985 | 3876652 | |
| role of lipopolysaccharide and complement in susceptibility of haemophilus ducreyi to human serum. | the role of lipopolysaccharide (lps) in the susceptibility of haemophilus ducreyi to human serum and the mechanism of complement activation by serum-susceptible (sers) strains were investigated. serum treated with 2 mm mg2+ and 20 mm ethylene glycol-bis(beta-aminoethyl ether)-n,n,n',n'-tetraacetic acid was nonbactericidal, but inulin-treated serum remained bactericidal. absorption of serum with heat-killed whole cells of an sers strain removed its bactericidal activity against the absorbing stra ... | 1985 | 3876995 |
| the comparative in-vitro activity of twelve 4-quinolone antimicrobials against haemophilus ducreyi. | we have compared the in-vitro activity of 12 quinolone antibiotics against 50 recent isolates of haemophilus ducreyi from the gambia. while these isolates were relatively resistant to the parent compounds, the newly synthesized quinolones exerted significant activity against h. ducreyi. ciprofloxacin and ofloxacin were the most active compounds (mic90 0.03 mg/l). | 1985 | 3877717 |
| clinical and microbiologic studies of genital ulcers in kenyan women. | the etiology of genital ulcers in women in tropical regions is poorly understood. eighty-nine women, presenting to a sexually transmitted disease clinic in nairobi (kenya) with a primary complaint of genital ulcers, were evaluated prospectively in a clinical and laboratory study. a final etiologic diagnosis was possible for 60 (67%) of the women. culture for haemophilus ducreyi was positive for 43 women, eight had secondary syphilis with ulcerated condyloma latum, three had primary syphilis, one ... | 1985 | 3878601 |
| molecular characterization of chloramphenicol-resistant haemophilus parainfluenzae and haemophilus ducreyi. | we examined chloramphenicol-resistant haemophilus parainfluenzae and haemophilus ducreyi strains isolated in various parts of the world. the antibiotic resistance determinants were located on conjugative plasmids in h. ducreyi, but were chromosomally located in h. parainfluenzae. both species produced chloramphenicol acetyltransferases (cats) that were sensitive to 5,5'-dithiobis(2-nitrobenzoic acid) like the enteric type ii and haemophilus influenzae cat enzymes, but differed from these enzymes ... | 1985 | 3879658 |
| typing haemophilus ducreyi by indirect immunofluorescence assay. | rabbits were inoculated with untreated whole cells of 16 strains of haemophilus ducreyi. homologous titres in an indirect immunofluorescence test ranged from 1/320 to 1/1280. no cross reactions, or very few, were observed between antisera to these strains and 22 other (possible taxonomically related) species, strains, and genera. a large diversity in types of antigen was observed among the strains of h ducreyi. antiserum from two antigens (35000 and 3138), however, reacted with all 16 h ducreyi ... | 1985 | 3884483 |
| haemophilus ducreyi. | haemophilus ducreyi is the etiologic agent of chancroid, an infection which is endemic in tropical and subtropical areas of the world and which is becoming more prevalent in europe and north america. recently, several strains have demonstrated resistance to several antimicrobials by plasmid acquisition and cell envelope alteration making control of the spread of this organism a challenge. equally challenging is the growth of this organism in the clinical microbiology laboratory because of its in ... | 1985 | 3888902 |
| prostitutes are a major reservoir of sexually transmitted diseases in nairobi, kenya. | prostitutes are a major reservoir of sexually transmitted diseases in many developing nations. in nairobi we found that 16%, 28%, and 46%, respectively, of upper-, middle-, and lower-social strata prostitutes were infected with neisseria gonorrhoeae. genital ulcers and infections with haemophilus ducreyi were more prevalent among prostitutes of the middle and lower social strata. a group of 97 prostitutes of the lower social strata were followed longitudinally to determine the rate of reinfectio ... | 1985 | 4002094 |
| the management of resistant chancroid in vietnam. | 1972 | 4537134 | |
| penile ulcer. | 1972 | 4538720 | |
| [haemophilus ducreyi: various metabolic and morphological aspects studied by radioactive precursors and electron microscopy]. | 1974 | 4546438 | |
| simplified laboratory technique for diagnosis of chancroid. | 1970 | 4914265 | |
| [multiple soft chancres of the free border of the prepuce in a portuguese with ducrey's bacilli (found again in auto-inoculation)]. | 1969 | 5313789 | |
| [a ghost: soft chancre. apropos of 2 recent cases]. | 1971 | 5314761 | |
| [laboratory diagnosis of soft chancre; difficulties]. | 1971 | 5314762 | |
| [reappearance of soft chancre (5 cases)]. | 1971 | 5314763 | |
| [recurrence of soft chancre. 2 recent cases]. | 1971 | 5316060 | |
| [reappearance of soft chancre (5 cases)]. | 1971 | 5316061 | |
| [epidemy of soft chancres (9 cases)]. | 1971 | 5316062 | |
| the role of haemophilus ducreyi in penile ulcers in bangkok, thailand. | in october 1982, penile ulcers were identified in 248 (19%) of 1282 men at bangrak hospital, bangkok, thailand. the etiology of the ulcers was determined for 120 (48%) of these patients. haemophilus ducreyi was isolated from 45 (38%), herpes simplex virus from 14 (12%), and neisseria gonorrhoeae from two (2%) of 120 patients. syphilis was detected in one patient. two patients had both h. ducreyi and herpes simplex virus isolated from the same ulcer. thirty-five per cent of patients had a single ... | 1984 | 6095473 |
| cefotaxime treatment of haemophilus ducreyi infection in kenya. | the authors conducted a double-blind randomized clinical trial comparing single-dose cefotaxime (1 g im) plus daily placebo injections with cefotaxime (1 g im on each of three days). each regimen was given with probenicid (1 g orally) for the treatment of chancroid. twenty haemophilus ducreyi culture-positive men received the single-dose cefotaxime regimen; in eight patients ulcers or buboes failed to respond to therapy. nineteen h. ducreyi culture-positive men received cefotaxime on each of thr ... | 1984 | 6098034 |
| modified haemin-containing medium for isolation of haemophilus ducreyi. | 1982 | 6120368 | |
| treatment of chancroid by clavulanic acid with amoxycillin in patients with beta-lactamase-positive haemophilus ducreyi infection. | multiresistant strains of haemophilus ducreyi, the aetiological agent of chancroid, are prevalent in nairobi, kenya, where tetracyclines and sulphonamides are no longer very effective in the treatment of chancroid. the following regimens (given three times daily for seven days) were compared in a double-blind randomised trial--amoxycillin 500 mg, amoxycillin 500 mg and clavulanic acid 125 mg, and amoxycillin 500 mg and clavulanic acid 250 mg. 68 of 100 ulcers were culture-positive for h. ducreyi ... | 1982 | 6125676 |
| chancroid. | 1982 | 6125814 |